CN107981837A - A kind of comparative study method that lighting source develops Macaque of Macaca axis oculi - Google Patents
A kind of comparative study method that lighting source develops Macaque of Macaca axis oculi Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000000052 comparative effect Effects 0.000 title claims abstract description 14
- 238000010171 animal model Methods 0.000 claims abstract description 51
- 230000007246 mechanism Effects 0.000 claims abstract description 49
- 241001465754 Metazoa Species 0.000 claims abstract description 36
- 230000000007 visual effect Effects 0.000 claims abstract description 26
- 230000004304 visual acuity Effects 0.000 claims abstract description 13
- 210000003128 head Anatomy 0.000 claims description 31
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- 230000004323 axial length Effects 0.000 claims description 9
- 210000002159 anterior chamber Anatomy 0.000 claims description 8
- 238000001228 spectrum Methods 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 5
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 239000005864 Sulphur Substances 0.000 claims description 3
- 230000037005 anaesthesia Effects 0.000 claims description 3
- 238000001949 anaesthesia Methods 0.000 claims description 3
- 230000036592 analgesia Effects 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
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- 230000006641 stabilisation Effects 0.000 claims description 2
- 238000011105 stabilization Methods 0.000 claims description 2
- 230000004515 progressive myopia Effects 0.000 abstract description 5
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- 241000282326 Felis catus Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
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- 238000013461 design Methods 0.000 description 2
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- A—HUMAN NECESSITIES
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- A61B3/00—Apparatus for testing the eyes; Instruments for examining the eyes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
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Abstract
The present invention relates to a kind of comparative study method that lighting source develops Macaque of Macaca axis oculi, this method are as follows：Multiple satisfactory Macaque of Macaca of visual indicator selective mechanisms value are chosen as experimental animal, and it is assigned at random and is positioned in N number of rearging cage of different lighting sources；Raising is after a week, detect each experimental animal ocular vision index initial detecting value, the experimental animal of initial detecting value and selective mechanisms value there are notable difference is excluded, detected value and continues to raise based on remaining experimental animal initial detecting value is averaged with selective mechanisms value；After raising 6 24 months, the final detected value of visual indicator of each experimental animal is detected；Compared and analyzed according to basic detected value and final detected value, obtain the analysis data that different lighting sources influence experimental animal visual acuity.There is no nocuity processing in experimentation of the present invention to animal, therefore be closer to the research method under natural myopia progression state.
Description
Technical field
Different illuminate to its axis oculi certainly is detected under the unartificial interference state of childhood Macaque of Macaca the present invention relates to one kind
The method of right development impact, belongs to photo-biological safety and photo-biological effect field.
Background technology
1. the excessive most important physiological performance for increasing the generation for being myopia of axis oculi.When the length of axis oculi is more than normal value
When, in the state of adjusting is loosened, parallel rays can not be focused on retina after the refraction of the dioptric system of eyeball, and
It is before converging at retina, causes not seeing Chu's distant place scenery, so as to produce myopia.This myopia is also referred to as axial myopia,
It is the myopic most important near-sighted type of students in middle and primary schools institute.It is generally believed that axis oculi development it is affected by many factors, wherein with light
According to relation it is the closest.
2. axis oculi is developed the most rapidly on the teenage individual of vertebrate, also it is easiest to be influenced by extraneous factor.Adult
Afterwards, organism axis oculi substantially no longer changes.It recent studies have shown that, the illumination that when people 3-8 Sui is received is different, it will greatly
Whether influencing the development of axis oculi and subsequently near-sighted generation.
3. in the zoopery of myopia studies, involved experimental animal mainly has chicken, cavy, big mouse, rabbit, macaque
Deng.Due to difference between species, the experimental result obtained on experimental animal not of the same race is often not consistent, it is also difficult to promotes
Onto people.Among these, since the affiliation of macaque and people are nearest, its vision system is similar with the height of people with ocellar structure.
Therefore, the experimental result obtained in macaque can be expanded to preferably on people.
4. in the conventional animal experiment of myopia studies, the age of experimental animal is mainly new born animal (chicken：Broken shell
Start to test within 7 days or so afterwards；Big mouse：Start to test after opening to eyes after birth；Macaque：Start within 30 days or so after birth real
Test).The method for the induction myopia taken mainly has：1) there is the eyelid of the one eye eyeball of suture experimental animal；2) in experimental animal
One eye eyeball on place concavees lens (optical defocus model)；3) diverging lens (shape is placed on the one eye eyeball of experimental animal
Feel deprivation model).The advantages of these conventional methods be can quick (several days to 30 days) Induction experiments animal axis oculi it is excessive
Increase, therefore can greatly accelerate experiment progress.But these traditional methods also have the shortcomings that obvious, predominantly：1)
The age bracket of animal is significantly less than the age bracket of people's myopia morbidity.These animals are in newborn after-vision system also in Rapid development
In the stage, starting myopia experiment at this time may be influenced by a variety of developmental factors；2) induced animal will be during producing myopia
Animal head either places concavees lens or diverging lens outside eyes, the process that this produces myopia with people is inconsistent.
5. the utility model patent of Patent No. 200620062570.4 discloses a kind of for inducing the inhuman spirit such as monkey long
The eyeshade of the myopia model of class.The principle of the eyeshade is to be based on optical defocus and form deprivation method.It is designed as circular helmet sample,
Each several part component is screwed, eyeglass is located at the front of eyeshade.Can be by lens rapid induction although the design is adapted to
The myopia of monkey, but this is different with the myopia progression process of monkey under natural conditions or people.And the patent is not to by lens
Light after deviation, blurring is measured and corrected, and the explanation of this mechanism that will be produced to myopia produces interference.
6. the patent of invention of Patent No. 201310238070.6 disclose it is a kind of for rabbit, cat, dog induction myopia
Wear mirror system and methods for using them.This, which wears mirror system, includes：Truncated cone-shaped mask, mirror support, induction eyeglass, fixed band and buckle realization,
Quick-replaceable can be carried out to eyeglass.It induces the principle of myopia to be also based on optical defocus and form deprivation method.The design is adapted to
Although can by lens rapid induction rabbit, cat, dog myopia, the myopia progression process of this and nature servant differs
Sample.And the patent is not also measured and corrected to the light after lens deviation, blurring, this will produce myopia
Mechanism explanation produce interference.
The content of the invention
The contrast developed the technical problem to be solved in the present invention is to provide a kind of lighting source to Macaque of Macaca axis oculi is ground
Study carefully method, this method need not place lens outside the eyes of experimental animal, not have nocuity processing to animal in experimentation,
The closer natural myopia progression state of experimental result.
In order to solve the above-mentioned technical problem, the comparative study side that lighting source of the invention develops Macaque of Macaca axis oculi
Method comprises the following steps：
Step 1: it is the healthy Macaque of Macaca of 3 months to 24 months to choose multiple ages, to its visual acuity state into
Row detection, therefrom filters out visual indicator and meets the Macaque of Macaca of requirement of experiment as experimental animal, while records each experiment
Animal vision index screening detected value Si, i is experimental animal numbering；
It is respectively placed in Step 2: the experimental animal filtered out is randomly divided into N groups in N number of rearging cage, N >=2, every group real
Size of animal is tested not less than 6；Then N-1 rearging cage is respectively placed in the raising room of different lighting sources to experiment
Animal is raised；One raising room uses natural lighting；
Step 3: raising is after a week, the visual acuity state of each experimental animal eyes is detected, it is dynamic to obtain each experiment
The visual indicator initial detecting value S of thing at this timei‘；Exclude initial detecting value Si' with selective mechanisms value SiThere are the reality of notable difference
Animal is tested, by remaining experimental animal initial detecting value Si' with selective mechanisms value SiIt is averaged the experimental animal for being i as numbering
Visual indicator basis detected value Zi, and continue to raise；
Step 4: raising 6-24 is detected the visual acuity state of each experimental animal eyes, obtains again after a month
The final detected value Z of visual indicator of each experimental animali‘；
Step 5: according to each laboratory animal visual index basis detected value ZiWith final detected value Zi' compare and analyze,
So as to obtain the analysis data that different lighting sources influence experimental animal visual acuity.
The lighting source include LED light, fluorescent lamp, incandescent lamp, halogen lamp, in electrodeless sulphur lamp two kinds and more than.
The illumination of center is 200-750lx in the rearging cage.
Between 20-25 degree, relative humidity controls between 40-70% temperature control in the raising room, ventilation
Number is not less than 8 times/hour.
The visual indicator is mainly axiallength.
The visual indicator can also include refraction of eye degree, anterior chamber depth, corneal curvature.
It is described that periodically the illumination in the spectrum and rearging cage of lighting source can be detected Step 3: in four, ensure
The stabilization of spectrum and illumination in experimentation；Meanwhile periodically retain the lighting source replaced in experiment as light source sample
Preserved.
Further, when being detected to Macaque of Macaca visual indicator, general anaesthesia is carried out to it first, treats that Macaca moves
After the reaction of thing analgesia, its head is fixed on position indicator and starts to detect towards detecting instrument.
If if Macaque of Macaca eyes close in detection process, the upper palpebra inferior of monkey is strutted using eye speculum, it is rear to carry out respectively
The inspection of index, periodically instills artificial tear to its ocular surface in checking process, ensures the moistening of its eyeball surface.
The position indicator includes head fixed mechanism, three-dimensional rotation fixing bracket, fixed collet；With solid on fixed collet
Rationed marketing and azimuth scale；Three-dimensional rotation fixing bracket is connected by the cylindrical aperture of bottom with fixed pin, can be made around fixed pin
Orientation is rotated and is locked；Three-dimensional rotation fixing bracket degree of rotation can be read by azimuth scale；Head fixed mechanism
Bottom is connected with the support bracket fastened top of three-dimensional rotation, can be made pitch relative to three-dimensional rotation fixing bracket and be rotated and lock
It is fixed；The degree of rotation of head fixed mechanism can be read by the pitching scale on three-dimensional rotation fixing bracket；Two ear bars and
Tooth support is installed on the fixed mechanism of head；In use, one end end of two ear bars can push against the porus acusticus externus and lock of animal ears
Fasten, tooth can be locked after propping up the upper front tooth of abutting in animal mouthful.
Two ear bars and tooth support are first removed during use, postanesthetic animal is crouched down on fixed collet, its head is put
It is placed in the centre of head fixed mechanism；Then two ear bars are put into the left frame and right frame of head fixed mechanism respectively, and
It is locked after the tapering point of two ear bars is pushed against the porus acusticus externus of animal ears.Tooth support stent is penetrated into head fixed mechanism
Right frame, while tooth is propped up in animal mouthful, by being locked after front tooth in abutting.Control head fixed mechanism makees orientation
And pitch rotation so that animal eyes are directed at the testing lens of inspection apparatus, and head fixed mechanism is locked at this time, passes through
The orientation degree of rotation and the pitch rotation number of degrees of azimuth scale and pitching scale reading head fixed mechanism simultaneously record；Pass through level
Scale reads the horizontal level and record of two ear bars, thus can complete to realize and the accurate adjustment of animal eyes and three-dimensional are determined
Position.
The present invention by detect raising standard environment under monkey axiallength change, react different lighting sources to axis oculi
The influence of development.Artificial light light source has perhaps many features, mainly has：Spatial distribution, colour temperature, brightness, colour rendering index, frequency
Dodge index etc..The various combination of these features is the key point that a kind of light source is different from other light sources.Since eyes are
Experience the organ of light, illumination of its growth course also in by environment is adjusted, therefore detects hair of any light source to eyeball
Educating the growth of especially axis oculi has the adjustment effect just very necessary.
The present invention utilization experimental animal Macaque of Macaca highly close with the vision system of people (such as：Rhesus macaque, food
Crab monkey etc.), research artificial light light source is developed by its axis oculi and the influence of myopia.This method is during experimental implementation, not specially
Door places concavees lens (negative lenses) or diverging lens (diffuser lenses) outside the eyes of experimental animal,
There is no nocuity processing in experimentation to animal, therefore be closer to the research method under natural myopia progression state.
Its head is fixed using position indicator when being detected to laboratory animal visual index, the position indicator is simple for structure,
Easy to use, registration, can reduce interference caused by eye position changes, realize the multiple checks number to same animal
According to stability.
Brief description of the drawings
The present invention is described in further detail with reference to the accompanying drawings and detailed description.
Fig. 1 is the animal eye examination stereotaxic instrument structure diagram of the present invention.
Fig. 2 is the side schematic view of the present invention.
Fig. 3 is the fixed pallet schematic diagram of the present invention.
In figure：1. head fixed mechanism, 11. bottom frames；12. left frame；13. right frame；2. three-dimensional rotation fixing bracket；
21. circular hole；22. pitching scale；23. cylindrical aperture；24th, 25 bolt；3. fix collet；31. fixed pin；32. azimuth scale；
41. ear bar；411. horizontal scale；42. bolt；43. ear bar；431. horizontal scale；44. bolt；45. tooth support；46. tooth support branch
Frame；47. bolt.
Embodiment
By taking macaque as an example, the comparative study method that lighting source of the invention develops Macaque of Macaca axis oculi is specifically such as
Under：
First, choose the healthy male rhesus macaque that the age is 24 months or so and carry out visual acuity state-detection, therefrom filter out
32 visual indicators meet the Macaque of Macaca of requirement of experiment as experimental animal, while record each laboratory animal visual index sieve
Selected Inspection measured value, including axiallength selective mechanisms value SALi, refraction of eye degree selective mechanisms value SDi, anterior chamber depth selective mechanisms value
SCDi, corneal curvature selective mechanisms value SKi；After statistical analysis, axiallength selective mechanisms value, refraction of eye degree selective mechanisms value,
Anterior chamber depth selective mechanisms value, corneal curvature selective mechanisms value think when the p value between standard value is more than or equal to 0.05
Meet requirement of experiment；Wherein i numbers for experimental animal.
2nd, 32 macaques are randomly divided into 4 groups, every group of 8 macaques, at this time can control disturbing factor to minimum.So
4 groups of macaques are respectively placed in 4 rearging cages afterwards, 4 rearging cages are respectively placed in 4 raising rooms to experimental animal
Raised；One of raising room uses natural lighting, in addition 3 raising rooms be respectively adopted LED light, fluorescent lamp,
Incandescent lamp.The illumination of center is 200-750lx in rearging cage, raises the temperature control in room between 20-25 degree, phase
To humid control between 40-70%, rate of ventilation is not less than 8 times/hour.Raising room-size is 5m × 3m × 2.6 meter.Room
Between four walls and ceiling be purification color steel, floor is light grey epoxide terrace paint ground.Activity space is 80cm in rearging cage
× 80cm × 80cm, total a height of 140cm, material is 304 brush finished stainless steels.
3rd, experimental animal can usually produce stress reaction under new illumination condition, according to different experiments animal to new ring
The difference of border adaptability, its stress reaction also have different, so as to influence the accuracy of experimental result；Therefore, one is raised
Zhou Hou, is detected the visual acuity state of each experimental animal eyes again, obtains the visual indicator of each experimental animal at this time
Initial detecting value；Including axiallength initial detecting value SALi', refraction of eye degree initial detecting value SDi', anterior chamber depth initial detecting
Value SCDi', corneal curvature initial detecting value SKi’；Wherein there are notable difference with corresponding selective mechanisms value for initial detecting value for exclusion
Experimental animal, detected value based on remaining experimental animal initial detecting value and selective mechanisms value are averaged, and continuing
Raised；Basic detected value includes axiallength basis detected value ZALi, refraction of eye degree basis detected value ZDi, anterior chamber depth base
Plinth detected value ZCDi, corneal curvature basis detected value ZKi；It is generally believed that the axiallength initial detecting value after statistical analysis
SALi' and selective mechanisms value SALiBetween, refraction of eye degree initial detecting value ZDiWith selective mechanisms value SDiBetween, anterior chamber depth it is initial
Detected value SCDi' and selective mechanisms value SCDiBetween or corneal curvature initial detecting value SKi' and selective mechanisms value SKiBetween p value
During less than 0.05, it is believed that there were significant differences between the two.
Step 4: after raising 6 months, the visual acuity state of each experimental animal eyes is detected again, is obtained each
The final detected value of visual indicator of experimental animal；Including axiallength basis detected value ZALI', refraction of eye degree basis detected value
ZDI', anterior chamber depth basis detected value ZCDI', corneal curvature basis detected value ZKI’；In feeding process, periodically to lighting source
Illumination in spectrum and rearging cage is detected, to ensure the stability of spectrum and illumination condition in experimentation；Meanwhile periodically
Retain the lighting source replaced in experiment to be preserved as light source sample, for experiment after, to experimental light sources
The optical parameters such as spectrum, brightness are remeasured, it is ensured that the uniformity of used lighting parameter in experiment.
Step 5: being compared and analyzed according to each group laboratory animal visual index basis detected value with final detected value, obtain
Obtain the analysis data that different lighting sources influence experimental animal visual acuity.
When being detected to macaque visual indicator, general anaesthesia is carried out to it first, after the reaction of macaque analgesia, by it
Head is fixed on position indicator and starts to detect towards detecting instrument.If if macaque eyes close in detection process, using opening
Eyelid device struts the upper palpebra inferior of macaque, carries out the inspection of indices afterwards, is periodically instilled in checking process to its ocular surface artificial
Tears, ensure the moistening of its eyeball surface.
As shown in the figure, the position indicator
Each rearging cage lighting source illumination parameter is shown in Table 1.
Table 1
Lighting source can also have halogen lamp, electrodeless sulphur lamp etc..
As shown in Figure 1, 2, 3, the position indicator includes head fixed mechanism 1, three-dimensional rotation fixing bracket 2, fixed collet
3。
Fixed pin 31 and azimuth scale 31 are carried on the fixed collet 3.
The three-dimensional rotation fixing bracket 2 is connected by the cylindrical aperture 23 of bottom with fixed pin 31, and bolt 25 is located at
In screw hole on three-dimensional rotation fixing bracket 2, three-dimensional rotation fixing bracket 2 and fixed collet 3 can be locked admittedly by bolt 25
It is fixed；After bolt 25 are unclamped, three-dimensional rotation fixing bracket 2 can make orientation rotation around fixed pin 31, and the number of degrees of rotation can the side of passing through
Position scale 31 is read.
Head fixed mechanism 1 can use rectangular frame；The bottom frame 11 of head fixed mechanism 1 is as pitch axis by three-dimensional
The circular hole 21 at the top of rotary fixed bracket 2 passes through；Bolt 24 is arranged in the screw hole on 2 top of three-dimensional rotation fixing bracket, can be incited somebody to action
Head fixed mechanism 1 is locked.When unclamping bolt 24, head fixed mechanism 1 can realize that pitch rotates, the angle of rotation
Degree can be read by the pitching scale 22 in the outside of circular hole 21.
The square two ear bars 41,43 in section are respectively by the left frame 12 and right frame 13 of head fixed mechanism 1
Square hole pass through, be locked by the bolt 42,44 being arranged in 13 threaded hole of left frame 12 and right frame；Two ear bars
41st, the 43 one end ends for pushing against animal ears porus acusticus externus are taper.Two ear bars 41,43 can left and right shifting when unclamping bolt 42,44
It is dynamic；Tooth support stent 46 is made of orthogonal two sections of straight-bars, and tooth support 45 is fixed therein the end of one section of straight-bar and towards rear
Side, can be put into animal mouthful and push against upper front tooth, and another section of straight-bar is used in combination by being passed through in the square hole of 1 right frame 13 of head fixed mechanism
Bolt 47 is fixed.
The head fixed mechanism 1 can also use other shapes that can fix animal head；Two ear bars 41,43 also may be used
To be connected using other modes with head fixed mechanism 1, as long as left and right horizontal movement can be made on head fixed mechanism 1 and locked
Fasten；Tooth support stent 46 can also be connected on bottom frame 11, and tooth support 45 is put into animal mouthful as long as can move back and forth
In middle abutting then front tooth is locked.
Claims (9)
1. a kind of comparative study method that lighting source develops Macaque of Macaca axis oculi, it is characterised in that comprise the following steps：
Step 1: choosing the healthy Macaque of Macaca that multiple ages are 3 months to 24 months, its visual acuity state is examined
Survey, therefrom filter out visual indicator and meet the Macaque of Macaca of requirement of experiment as experimental animal, while record each experimental animal
Visual indicator selective mechanisms value Si, i is experimental animal numbering；
It is respectively placed in Step 2: the experimental animal filtered out is randomly divided into N groups in N number of rearging cage, N >=2, every group of experiment is moved
Thing quantity is not less than 6；Then N-1 rearging cage is respectively placed in the raising room of different lighting sources to experimental animal
Raised；One raising room uses natural lighting；
Step 3: raising after a week, the visual acuity state of each experimental animal eyes is detected, obtain each experimental animal this
When visual indicator initial detecting value Si‘；Exclude initial detecting value Si' with selective mechanisms value SiExperiment there are notable difference is moved
Thing, by remaining experimental animal initial detecting value Si' with selective mechanisms value SiIt is averaged the laboratory animal visual for being i as numbering
Index basis detected value Zi, and continue to raise；
Step 4: raising 6-24 is detected the visual acuity state of each experimental animal eyes, obtains each reality again after a month
Test the final detected value Z of visual indicator of animali‘；
Step 5: according to each laboratory animal visual index basis detected value ZiWith final detected value Zi' compare and analyze, so that
Obtain the analysis data that different lighting sources influence experimental animal visual acuity.
2. the comparative study method that lighting source according to claim 1 develops Macaque of Macaca axis oculi, its feature exist
In the lighting source include LED light, fluorescent lamp, incandescent lamp, halogen lamp, in electrodeless sulphur lamp two kinds and more than.
3. the comparative study method that lighting source according to claim 1 develops Macaque of Macaca axis oculi, its feature exist
Illumination in center in the rearging cage is 200-750lx.
4. the comparative study method that lighting source according to claim 1 develops Macaque of Macaca axis oculi, its feature exist
In the temperature control in the raising room between 20-25 degree, relative humidity is controlled between 40-70%, and rate of ventilation is not
Less than 8 times/hour.
5. the comparative study method that lighting source according to claim 1 develops Macaque of Macaca axis oculi, its feature exist
In the visual indicator be mainly axiallength.
6. the comparative study method that lighting source according to claim 5 develops Macaque of Macaca axis oculi, its feature exist
Refraction of eye degree, anterior chamber depth, corneal curvature are further included in the visual indicator.
7. the comparative study method that lighting source according to claim 1 develops Macaque of Macaca axis oculi, its feature exist
Periodically the illumination in the spectrum and rearging cage of lighting source is detected Step 3: in four in described, is ensured in experimentation
The stabilization of spectrum and illumination；Meanwhile periodically retain the lighting source replaced in experiment and preserved as light source sample.
8. the comparative study method that lighting source according to claim 1 develops Macaque of Macaca axis oculi, its feature exist
When being detected to Macaque of Macaca visual indicator, general anaesthesia is carried out to it first, treats that Macaque of Macaca analgesia reacts
Afterwards, its head is fixed on position indicator and starts to detect towards detecting instrument.
9. the comparative study method that lighting source according to claim 8 develops Macaque of Macaca axis oculi, its feature exist
Include head fixed mechanism (1), three-dimensional rotation fixing bracket (2), fixed collet (3) in the position indicator；On fixed collet (3)
With fixed pin (31) and azimuth scale (32)；Three-dimensional rotation fixing bracket (2) is by the cylindrical aperture (23) of bottom with consolidating
Rationed marketing (31) is connected, and can make orientation around fixed pin (31) and rotate and be locked；Three-dimensional rotation fixing bracket (2) degree of rotation can
Read by azimuth scale (32)；The bottom of head fixed mechanism (1) is connected with the top of three-dimensional rotation fixing bracket (2), can
Make pitch relative to three-dimensional rotation fixing bracket (2) to rotate and be locked；The degree of rotation of head fixed mechanism (1) can
Read by the pitching scale (22) on three-dimensional rotation fixing bracket (2)；Two ear bars (41), (43) and tooth support (45) are installed on
On head fixed mechanism (1)；In use, two ear bars (41), one end end of (43) can push against the porus acusticus externus of animal ears simultaneously
It is locked, tooth support (45) can be locked after being put into the upper front tooth of abutting in animal mouthful.
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| CN113615641A (en) * | 2021-08-23 | 2021-11-09 | 浙江大学 | Method for artificially feeding newborn marmosets |
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| CN107981837B (en) | 2020-04-28 |
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