CN107980509B - Method for improving yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin - Google Patents
Method for improving yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin Download PDFInfo
- Publication number
- CN107980509B CN107980509B CN201711299842.1A CN201711299842A CN107980509B CN 107980509 B CN107980509 B CN 107980509B CN 201711299842 A CN201711299842 A CN 201711299842A CN 107980509 B CN107980509 B CN 107980509B
- Authority
- CN
- China
- Prior art keywords
- gibberellin
- indigenous bacteria
- producing
- rape
- cabbage type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Forests & Forestry (AREA)
- Virology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Ecology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a method for improving the yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin, which is to check local weather forecast and ensure that the method is implemented without raining within 48 hours. And spraying flowers and carob fruits of the cabbage type yellow seed rape 3-15 days after the first flowering by using a mixed solution of indigenous bacteria capable of producing gibberellin. After the seeds are ripe and harvested, the contents of anthocyanidin and proanthocyanidin in the seed coats of the seeds are respectively reduced by 25-45% and 50-60%, and the yellow seed rate can be improved by 30-45%.
Description
Technical Field
The invention relates to the technical field of agricultural application biology, in particular to a method for improving the yellow seed rate of brassica napus by utilizing indigenous bacteria producing gibberellin.
Background
Research shows that, compared with black-seed rape, yellow-seed rape has the advantages of thin seed coat, high oil content, high protein content, less pigment in oil, transparency, etc. under the same genetic background. Because the phenomenon that yellow seeds are unstable in character and the yellow seed rate is reduced, and some yellow seeds can be reversed to black seeds, and the like exists in the cabbage type rape, a certain cultivation management measure is adopted to obtain a rape product with less pigments in seed coats and high yellow seed rate, and the rape product is one of the problems concerned in rape cultivation.
From the development and composition of rapeseeds, the embryo of the yellow-seed rape or black-seed rape is yellow, so that the yellow-seed rate of the rape depends on the color of the seed coat. Previous studies reported the timing of changes in seed coat color and the spatiotemporal differences in seed coat color in oilseed rape, but did not find the underlying cause of seed coat color differences. Research reports that the difference of seed coat color during seed development is caused by polyphenols; we found that anthocyanidin, proanthocyanidin and the like in the seed coat are the main causes of the color difference of the seed coat of rape. Pigments in plants are formed through a series of biosynthetic pathways, the synthesis of pigments in seed coats is mutually related to metabolic processes of other substances, each metabolic process needs catalysis of enzyme, each metabolism generates specific metabolites, and the related metabolites can influence the phenotype of the seed coat color of rape. The metabolic processes of different substances have certain space-time characteristics, and the metabolic products at corresponding stages can be regulated and controlled by biochemical reagents at different development times.
In plants, studies have shown that phytohormones can regulate the color of plants, and that the effects of the same hormone on color at different developmental stages of plants are different. When studying the biological pathways for synthesizing the seed coat anthocyanidin, proanthocyanidin and the like of rape, the inventor finds that if the upstream products of the pathways are reduced, the downstream synthesized products are correspondingly reduced. We have found that during the early development of rape seeds, lower concentrations of gibberellin (GA 3) can inhibit the expression of some genes in the anthocyanidin and proanthocyanidin synthesis pathways, thereby reducing the anthocyanidin, proanthocyanidin content in the seed coat. Researches show that the color of seed coats can be lightened or even transparent by spraying the indigenous bacteria mixed solution for producing gibberellin at the early flowering stage of the rape, so that the yellow seed rate of the rape is improved.
Disclosure of Invention
The invention aims to provide a method for improving the yellow seed rate of cabbage type rape, which can regulate the expression of genes related to seed coat color formation, reduce the synthesis of pigments, reduce the contents of anthocyanins and proanthocyanidins in the seed coats and increase the transparency of the seed coats, thereby promoting the formation of the yellow seeds of the rape and improving the quality of rapeseeds.
The technical scheme of the invention is as follows: a method for improving the yellow seed rate of Brassica napus by using indigenous bacteria producing gibberellin comprises the following steps:
1) obtaining of gibberellin-producing indigenous bacteria: separating and identifying the well-grown brassica plant body and the surrounding soil by adopting a conventional method to obtain the indigenous bacteria for producing gibberellin;
2) and (3) expanding culture of the gibberellin-producing indigenous bacteria: liquid culture is carried out on the indigenous bacteria producing gibberellin; the components of the culture medium are as follows: 1% of glucose, 1.5% of corn flour, 1.1% of KH2PO40.1%, 0.1% of MgSO40.1%, 0.02% of boric acid, 0.2% of glycerol, 60.05% of vitamin B and 0.1% of peptone, adjusting the pH value to 4.0, and sterilizing for later use; inoculating indigenous bacteria producing gibberellin, and culturing at 28 deg.C for 2-3 days;
3) filtering the culture solution, diluting by 5-10 times according to the concentration of bacteria, and adjusting pH to 5.5-6.5 for later use, referred to as a stock solution;
4) selecting proper climatic conditions, spraying the standby liquid under the condition of ensuring that no rain falls within 48 hours after the method is implemented, uniformly spraying flowers and pods of the cabbage type rape 3-15 days after the plants bloom for the first time, and just seeing the water drops formed on the flowers and the pods.
Preferably, in step (3), the culture solution is diluted 8-fold after filtration and is adjusted to pH 6 for use.
Has the advantages that:
(1) after the seeds of the cabbage type yellow seed rape material treated by the method are ripe and harvested, the contents of anthocyanidin and proanthocyanidin in the seed coat are respectively reduced by 25-45% and 50-60%, and the yellow seed rate can be improved by 30-45%.
(2) The invention is found based on the previous research results of the inventor, has simple operation, small used reagent amount and low cost, has obvious effect of reducing the pigment content of rape seed skin, and can improve the yellow seed rate of rape so as to improve the quality of rape oil.
Detailed Description
Example 1
Obtaining of gibberellin-producing indigenous bacteria: separating and identifying the well-grown Brassica Chinese cabbage and the surrounding soil thereof by using a conventional method to obtain the indigenous bacteria for producing gibberellin.
And (3) expanding culture of the gibberellin-producing indigenous bacteria: the above-mentioned indigenous bacteria producing gibberellin are subjected to liquid culture. The components of the culture medium are as follows: 1% of glucose, 1.5% of corn flour, 1.1% of KH2PO40.1%, 0.1% of MgSO40.1%, 0.02% of boric acid, 0.2% of glycerol, 60.05% of vitamin B and 0.1% of peptone, adjusting the pH value to 4.0, and sterilizing for later use. Inoculating indigenous bacteria producing gibberellin, shaking slowly with a shaker, and culturing at 28 deg.C for 3 days. Filtering the culture solution with sterile gauze, diluting by 7 times according to the concentration of bacteria, and adjusting pH to 5.5-6.5 for use, referred to as stock solution.
Example 2
The cabbage type yellow seed rape plants potted under natural conditions are taken, the standby liquid in the embodiment 1 is taken when the plants bloom for the first time for 3 days, rape flowers and carob fruits are sprayed, and the local weather condition is no rain within 48 hours. After the seeds are ripe and harvested, the content of anthocyanidin and proanthocyanidin in the seed coat is reduced by 30% and 53% respectively through detection, and the yellow seed rate is improved by 42%.
Example 3
The cabbage type yellow seed rape plants potted under natural conditions are taken, the standby liquid in the embodiment 1 is taken when the plants bloom for the first time for 5 days, rape flowers and carob fruits are sprayed, and the local weather condition is no rain within 48 hours. After the seeds are ripe and harvested, the content of anthocyanidin and proanthocyanidin in the seed coat is reduced by 41 percent and 60 percent respectively through detection, and the yellow seed rate is improved by 45 percent.
Example 4
0.1 mu of cabbage type yellow seed rape field block is taken, the standby liquid in the embodiment 1 is taken when the plants bloom for 15 days for the first time, rape flowers and hornberries are sprayed, and the local weather condition is no rain within 48 hours. After the seeds are ripe and harvested, the content of anthocyanidin and proanthocyanidin in the seed coat is respectively reduced by 27% and 50% through detection, and the yellow seed rate is improved by 33%.
Example 5
0.1 mu of cabbage type yellow seed rape field block is taken, the standby liquid in the embodiment 1 is taken when the plants bloom for the first time for 7 days, rape flowers and hornberries are sprayed, and the local weather condition is no rain within 48 hours. After the seeds are ripe and harvested, the content of anthocyanidin and proanthocyanidin in the seed coat is respectively reduced by 45% and 58% through detection, and the yellow seed rate is improved by 41%.
Claims (1)
1. A method for improving the yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin is characterized by comprising the following steps:
(1) obtaining of gibberellin-producing indigenous bacteria: separating and identifying the well-grown brassica plant body and the surrounding soil by adopting a conventional method to obtain the indigenous bacteria for producing gibberellin;
(2) and (3) expanding culture of the gibberellin-producing indigenous bacteria: liquid culture is carried out on the indigenous bacteria producing gibberellin; the components of the culture medium are as follows: 1% of glucose, 1.5% of corn flour, 1.1% of KH2PO40.1%, 0.1% of MgSO40.1%, 0.02% of boric acid, 0.2% of glycerol, 60.05% of vitamin B and 0.1% of peptone, adjusting the pH value to 4.0, and sterilizing for later use; inoculating indigenous bacteria producing gibberellin, and culturing at 28 deg.C for 2-3 days;
(3) filtering the culture solution, diluting by 8 times according to the concentration of bacteria, and adjusting the pH value to 6 for later use, referred to as a stock solution;
(4) selecting proper climatic conditions, spraying the standby liquid under the condition of ensuring that no rain falls within 48 hours after the method is implemented, uniformly spraying flowers and pods of the cabbage type rape 3-15 days after the plants bloom for the first time, and just seeing the water drops formed on the flowers and the pods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711299842.1A CN107980509B (en) | 2017-12-09 | 2017-12-09 | Method for improving yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711299842.1A CN107980509B (en) | 2017-12-09 | 2017-12-09 | Method for improving yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107980509A CN107980509A (en) | 2018-05-04 |
| CN107980509B true CN107980509B (en) | 2020-10-02 |
Family
ID=62035900
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711299842.1A Active CN107980509B (en) | 2017-12-09 | 2017-12-09 | Method for improving yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107980509B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1282413C (en) * | 2003-11-05 | 2006-11-01 | 西南农业大学 | Cabbage type yellow seed rape grain color controlling method |
| CN1644029A (en) * | 2005-01-17 | 2005-07-27 | 西南农业大学 | Complete dominant rape seed screening and parent selecting and matching method |
| CN101731273B (en) * | 2009-12-21 | 2012-11-21 | 华中农业大学 | Seed treatment agent suitable for quick sprouting of rapes |
| CN103804096B (en) * | 2014-02-26 | 2015-12-02 | 扬州大学 | A kind of promote rape machine transplanting of rice blanket seedling early to live treatment agent and application method |
-
2017
- 2017-12-09 CN CN201711299842.1A patent/CN107980509B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107980509A (en) | 2018-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Feng et al. | Influence of cluster zone leaf removal on Pinot noir grape chemical and volatile composition | |
| Zarrouk et al. | Impact of irrigation regime on berry development and flavonoids composition in Aragonez (Syn. Tempranillo) grapevine | |
| Pavoković et al. | Complex biochemistry and biotechnological production of betalains | |
| Kolayli et al. | Comparative study of chemical and biochemical properties of different melon cultivars: Standard, hybrid, and grafted melons | |
| Ruedell et al. | Pre and post-severance effects of light quality on carbohydrate dynamics and microcutting adventitious rooting of two Eucalyptus species of contrasting recalcitrance | |
| Deng et al. | SUNRED, a natural extract-based biostimulant, application stimulates anthocyanin production in the skins of grapes | |
| Babalık et al. | Brassinosteroids modify yield, quality, and antioxidant components in grapes (Vitis vinifera cv. Alphonse Lavallée) | |
| Theodorou et al. | Anthocyanin content and composition in four red winegrape cultivars (Vitis vinifera L.) under variable irrigation: Anthocyanin content and composition under variable irrigation | |
| Ghanem et al. | Effect of root colonization with Piriformospora indica and phosphate availability on the growth and reproductive biology of a Cyclamen persicum cultivar | |
| CN105379571B (en) | A kind of method of anthocyanin accumulation in promotion Roselle calyx | |
| Belal et al. | Using brassinolide and girdling combined application as an alternative to ethephon for improving color and quality of'Crimson Seedless' grapevines | |
| CN107980509B (en) | Method for improving yellow seed rate of cabbage type rape by utilizing indigenous bacteria producing gibberellin | |
| Al-Mohammad et al. | Effect of phenylalanine, jasmonic acid and biofertilizer on growth, yield and anthocyanin pigments of roselle calyces | |
| Pi et al. | Transient expression assay in strawberry fruits | |
| Kinsou et al. | Effet de la salinité sur la floraison, la fructification et la qualité nutritionnelle des fruits du cultivar local Akikon de tomate (Lycopersicon esculentum Mill.) du Bénin | |
| Makhoul et al. | Some chemical characteristics of white (Morus alba L.) and black (Morus nigra L) mulberry phenotypes in Tartus Syria | |
| Cüce et al. | Phenolic constituents of Vaccinium species from both natural resources and micropropagated plantlets | |
| CN105340753A (en) | High-starch-yield potato tissue culture method | |
| Medina et al. | Induction of Anthocyanin Production in Established Callus Cultures of Roselle (Hibiscus sabdariffa L.) Using Yeast Extract | |
| ANTOCE et al. | The effect of green harvest on the quality of organic grapes cultivated in Murfatlar viticultural centre. | |
| Posaluk et al. | The Effects of Bio-extract from water hyacinth (Eichhornia crassipes (C. Mart.) Solms) and Golden Apple Snail (Pomacea canaliculate Lamarck) on photosynthetic Pigment and Ascorbic Acid Contents of Chinese Cabbage (Brassica chinensis var. pekinensis Rupr.) Grown in Hydroponic Culture. NU | |
| CN109006261A (en) | A method of improving violet cabbage anthocyanidin content | |
| CN102318512B (en) | Rape seed husk color regulation and control method | |
| KR100292291B1 (en) | How to produce plant tissues containing large amounts of natural anthocyanins using white plants | |
| Korkutal et al. | Effects of early water stress levels on berry set and berry development in Merlot cv.(Vitis vinifera L.) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |