CN107976439B - A kind of optical detection apparatus detecting blood and urine sample - Google Patents
A kind of optical detection apparatus detecting blood and urine sample Download PDFInfo
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- CN107976439B CN107976439B CN201711272800.9A CN201711272800A CN107976439B CN 107976439 B CN107976439 B CN 107976439B CN 201711272800 A CN201711272800 A CN 201711272800A CN 107976439 B CN107976439 B CN 107976439B
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- 230000003287 optical effect Effects 0.000 title claims abstract description 66
- 238000001514 detection method Methods 0.000 title claims abstract description 41
- 239000008280 blood Substances 0.000 title claims abstract description 29
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- 210000002700 urine Anatomy 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000012360 testing method Methods 0.000 claims abstract description 12
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 claims description 40
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 claims description 32
- 229940109239 creatinine Drugs 0.000 claims description 20
- 239000006046 creatine Substances 0.000 claims description 16
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- 239000008363 phosphate buffer Substances 0.000 claims description 6
- 229940075930 picrate Drugs 0.000 claims description 6
- OXNIZHLAWKMVMX-UHFFFAOYSA-M picrate anion Chemical compound [O-]C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-M 0.000 claims description 6
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- 239000004328 sodium tetraborate Substances 0.000 description 5
- 235000010339 sodium tetraborate Nutrition 0.000 description 5
- 210000003734 kidney Anatomy 0.000 description 4
- QOMNQGZXFYNBNG-UHFFFAOYSA-N acetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-n-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate Chemical compound CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=CC=C(C)C=C1OCCOC1=CC(C2=C3C=C(F)C(=O)C=C3OC3=CC(OCOC(C)=O)=C(F)C=C32)=CC=C1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O QOMNQGZXFYNBNG-UHFFFAOYSA-N 0.000 description 3
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses a kind of detection blood and urine sample optical detection apparatus, belongs to field of optical detection, including shade guide teeth, sample injector, detector and detection terminal.Blood or urine sample can be tested using a variety of methods simultaneously, design has double light path, avoids the unstability using single method measurement, avoids in the prior art using the defect of the detection methods detectable concentration result inaccuracy such as test paper；It can choose using colorimetric method or Fluorometric assay blood or urine sample；Present invention design is portable to make patient be in Determination of The Proper Motions, then judges whether to need to see a doctor.
Description
Technical field
The invention belongs to field of optical detection, are related to medicinal blood and urine optical detection apparatus, and in particular to Yi Zhongjian
Survey blood and urine sample optical detection apparatus.
Background technique
Chronic renal failure refers to that various kidney troubles lead to the gradual irreversibility decline of renal function, until function loses institute
Clinical syndrome composed by a series of symptoms of appearance and metabolic disorder, abbreviation chronic kidney hypofunction.The terminal phase of chronic kidney hypofunction is
For it has often been said that uremia.A uremia not instead of independent disease, the clinic that the kidney trouble in various advanced stages is shared are comprehensive
Simulator sickness is that chronic renal failure enters syndrome composed by a series of clinical manifestations occurred when the terminal stage.
In the treatment of practical person having renal failure, most important method is haemodialysis.It is needed before dialysing to patient
Measure the blood creatinine content of patient, it is considered that dialyse when blood creatinine content is more than 700 μm of ol/L.Disease
People carries out that blood creatinine content will be measured before dialysing every time, very inconvenient.There are no one kind to allow in the prior art
Patient, which is in, oneself measures the detector of blood and urine creatinine content.In addition, the measuring method of creatinine content has bitter taste at present
Hydrochlorate method, hydrolyzes enzyme process and enzymatic measurement, and every kind of measuring method has certain detection error, such as patient is currently in use
Drug may impact certain detection method.Creatinine content determination device on the market can only use a kind of mode at present
Creatinine content is measured, creatinine content cannot be measured with a variety of methods simultaneously.
Summary of the invention
The present invention is directed to the disadvantages described above of the prior art, and devising one kind can be simultaneously using a variety of methods to blood and urine
The device that liquid sample is measured, it is specific as follows
A kind of detection blood and urine sample optical detection apparatus, it is characterised in that including shade guide teeth, sample injector, detector
And detection terminal；
The shade guide teeth is layer structure, can accommodate sample to be tested in layer structure；The sample injector and detector can
Shade guide teeth to be inserted into, sample is added in comparison color chips after shade guide teeth is inserted by sample injector and shade guide teeth is inserted by reagent, detector
Sample is detected afterwards；Wherein detector is that multiple light courcess, multi-pass are arranged, and when use can choose monochromatic light source module or more
Light source mode；Wherein monochromatic light source module is used for fluorescence detection method, and multiple light courcess mode is used for delustring colorimetric determination；The detection
Terminal acquisition testing image calculates gray value of image and obtains the concentration of sample to be tested.
The shade guide teeth is made of trilaminate material, respectively transparent underlayer, comb shape interlayer, transparent overlay, the clear bottom
Layer and transparent overlay are transparent fused silica glass material, and comb shape sandwich material is PMMA, and whole is in comb shape；The comb shape interlayer
One end has comb shape tooth, has the sample cavity that can accommodate sample when detecting between comb shape tooth；Sample cavity is connect with the external world
Inlet is sealed using container is blocked；The closure container is made of thin-film material, and inside accommodating can be with example reaction
Reagent or normal concentration standard sample；The closure container of the inlet setting of the sample cavity can be one or two；
The sample injector appearance is a cuboid, and top is equipped with the addition pool that sample to be tested can be added, and side wall offers
The sample injector socket that shade guide teeth can be inserted into, sets sample injector channel in sample injector socket, sample injector channel has with horizontal plane
Angle obliquely, sample injector channel end and addition pool connection, sample-adding bottom of pond portion are set as downward tapered hole, the tapered hole
For puncturing the closure container after shade guide teeth is inserted into sample injector socket, so that the sample cavity of shade guide teeth and addition pool connection
Sample and the reagent blocked in container is set to flow into sample cavity simultaneously；
The detector appearance is a cuboid, and side wall is equipped with the detector socket that can be inserted into shade guide teeth, detection
Detector channel is set in device socket, detector channel and horizontal plane have angle obliquely；
Another side wall opposite with detector socket is provided with first light source socket, the first light source on the detector
First light source can be inserted in socket, and the first light source is made of multiple photophores arranged side by side, the wavelength of multiple photophores
It is different；It is provided with first level optical channel in first light source socket, multiple optical waveguides are provided in first level optical channel,
Optical waveguide quantity is identical as photophore quantity, and the light level that optical waveguide is used to issue photophore is propagated, and is independent of each other；First water
Zero diopter channel end is located at below detector channel, and first level optical channel end is provided with semi-transparent semi-reflecting lens and vertical light is logical
Road, vertical optical channel pass through detector channel, and semi-transparent semi-reflecting lens are used to straight up reflect the light that the first photophore issues, the
The light that one photophore issues is emitted after passing through sample cavity from the exit portal of detector upper surface；
Second light source socket is provided on the detector on the side wall vertical with first level optical channel, second light source is inserted
Second light source can be inserted in mouth, and the second light source is single wavelength light source；Second horizontal optical channel is set in second light source socket,
Second horizontal optical channel is vertical with first level optical channel；The level height of second horizontal optical channel is logical lower than first level light
Road, the second horizon light channel end are located at immediately below vertical optical channel and are connected to vertical optical channel, the second horizontal optical channel end
End is provided with N number of transflection mirror, and the reflectivity of the transflection mirror farthest apart from second light source is 1, penultimate transflection mirror
Reflectivity is 1/2, and so on, the reflectivity of the transflection mirror nearest apart from second light source is 1/N；N number of transflection mirror is used for the
It is reflected straight up after the light N equal part that two light sources issue, the light that the second photophore issues table from detector after sample cavity
The exit portal in face is emitted；
The exit portal is set as multiple, and individual optical filter can be inserted in each exit portal.
The detection terminal setting has image collecting device and image processing apparatus, when image acquisition device detects
Exit portal image, image processing apparatus carry out image segmentation to exit portal image, are divided into standard items image and product to be tested image,
The standard items image and product to be tested image are converted into gray value, the gray value of standard of comparison product image and product to be tested image,
The concentration of product to be tested is determined according to gray value ratio.
The reagent stored in the closure container includes: creatine concentration is that Standard blood sample, the creatinine of 700 μm of ol/L is dense
Degree is that Standard blood sample, the creatine concentration that standard urine sample, the creatine concentration of 700 μm of ol/L is 0 μm of ol/L are 0 μm of ol/L
Standard urine sample, picrate, SDS, borax, sarcosine oxidase, phosphate buffer, 4-AA, anti-
Bad hematic acid oxidizing ferment, potassium ferrocyanide, Creatininase, kreatinase, peroxidase, Sodium azide, creatinine hydrase, creatine kinase,
Or mixtures thereof one of pyruvate kinase, lactic dehydrogenase.
When test object is creatinine, multiple photophores of the first light source issue light wavelength include 510nm,
545nm、340nm。
The invention has the benefit that
Blood or urine sample can be tested using a variety of methods simultaneously, be avoided using single method measurement
Unstability；Test object of the present invention is liquid, is avoided in the prior art using detection methods detectable concentration results such as test paper
The defect of inaccuracy；Present invention design has double light path, can choose using colorimetric method or Fluorometric assay blood or urine
Sample；Present invention design is portable can to make patient be in Determination of The Proper Motions, then judges whether to need to see a doctor.
Detailed description of the invention
Fig. 1 shade guide teeth schematic device of the present invention；
Fig. 2 sample injector schematic device of the present invention；
Fig. 3 detector means schematic top plan view of the present invention；
Fig. 4 detector first level optical channel schematic diagram of the present invention；
Fig. 5 the second horizon light of detector access diagram of the present invention.
Specific embodiment
Embodiment 1:
A kind of detection blood and urine sample optical detection apparatus, it is characterised in that including shade guide teeth 1, sample injector 2, detection
Device 3 and detection terminal；
The shade guide teeth is layer structure, can accommodate sample to be tested in layer structure；The sample injector and detector can
Shade guide teeth to be inserted into, sample is added in comparison color chips after shade guide teeth is inserted by sample injector and shade guide teeth is inserted by reagent, detector
Sample is detected afterwards；Wherein detector is that multiple light courcess, multi-pass are arranged, and when use can choose monochromatic light source module or more
Light source mode；Wherein monochromatic light source module is used for fluorescence detection method, and multiple light courcess mode is used for delustring colorimetric determination；The detection
Terminal acquisition testing image calculates gray value of image and obtains the concentration of sample to be tested.
The shade guide teeth is made of trilaminate material, respectively transparent underlayer 103, comb shape interlayer 102, transparent overlay 101, institute
It states transparent underlayer 103 and transparent overlay 101 is transparent fused silica glass material, 102 material of comb shape interlayer is PMMA, comb shape folder
Whole layer is in comb shape；Described 102 one end of comb shape interlayer has comb shape tooth, and sample can be accommodated when detecting by having between comb shape tooth
Sample cavity 105；The inlet that sample cavity is connect with the external world is sealed using container 104 is blocked；The closure container uses film
Material is made, and inside accommodating can be with the reagent of example reaction or the standard sample of normal concentration；The entrance of the sample cavity
The closure container of place's setting can be one or two；
2 appearance of sample injector is a cuboid, and top is equipped with the addition pool 201 that sample to be tested can be added, and side wall is opened
Equipped with the sample injector socket 202 that can be inserted into shade guide teeth, sample injector channel, sample injector channel and level are set in sample injector socket
Face has angle obliquely, sample injector channel end and addition pool connection, and sample-adding bottom of pond portion is set as downward tapered hole, described
Tapered hole is used to puncture the closure container after shade guide teeth is inserted into sample injector socket, so that the sample cavity and addition pool of shade guide teeth
Sample and the reagent blocked in container is set to flow into sample cavity while connection；
The detector appearance is a cuboid, and side wall is equipped with the detector socket 320 that can be inserted into shade guide teeth, inspection
It surveys in device socket and sets detector channel, detector channel and horizontal plane have angle obliquely；
Another side wall opposite with detector socket is provided with first light source socket, the first light source on the detector
First light source 301 can be inserted in socket, and the first light source is made of multiple photophores arranged side by side, the wave of multiple photophores
Length is different；It is provided with first level optical channel 302 in first light source socket, multiple light are provided in first level optical channel
Waveguide 303, optical waveguide quantity is identical as photophore quantity, the light level propagation that optical waveguide is used to issue photophore, mutually not shadow
It rings；First level optical channel end is located at below detector channel, and first level optical channel end is provided with semi-transparent semi-reflecting lens 304
With vertical optical channel, vertical optical channel passes through detector channel, and the light that semi-transparent semi-reflecting lens are used to issue the first photophore is vertical
Reflection upwards, the light that the first photophore issues are emitted after passing through sample cavity from the exit portal 305 of detector upper surface；
Second light source socket is provided on the detector on the side wall vertical with first level optical channel, second light source is inserted
Second light source 311 can be inserted in mouth, and the second light source is single wavelength light source；The second horizon light of setting is logical in second light source socket
Road 312, the second horizontal optical channel are vertical with first level optical channel；The level height of second horizontal optical channel is lower than first level
Optical channel, the second horizon light channel end are located at immediately below vertical optical channel and are connected to vertical optical channel, and the second horizon light is logical
Road end is provided with N number of transflection mirror 313, and the reflectivity of the transflection mirror farthest apart from second light source is 1, and penultimate is saturating
The reflectivity of anti-mirror is 1/2, and so on, the reflectivity of the transflection mirror nearest apart from second light source is 1/N；N number of transflection mirror is used
It is reflected straight up after the light N equal part for issuing second light source, the light that the second photophore issues is after sample cavity from detection
The exit portal of device upper surface is emitted；
The exit portal 305 is set as multiple, and individual optical filter can be inserted in each exit portal.
The detection terminal setting has image collecting device and image processing apparatus, when image acquisition device detects
The image of exit portal, image processing apparatus carry out image segmentation to the image of exit portal, are divided into standard items image and product to be tested
The standard items image and product to be tested image are converted to gray value, the ash of standard of comparison product image and product to be tested image by image
Angle value determines the concentration of product to be tested according to gray value ratio.
The reagent stored in the closure container are as follows: creatine concentration is Standard blood sample, the creatine concentration of 700 μm of ol/L
Standard urine sample, creatine concentration for 700 μm of ol/L are that Standard blood sample, the creatine concentration of 0 μm of ol/L is 0 μm of ol/L
It is standard urine sample, picrate, SDS, borax, sarcosine oxidase, phosphate buffer, 4-AA, anti-bad
Hematic acid oxidizing ferment, potassium ferrocyanide, Creatininase, kreatinase, peroxidase, Sodium azide, creatinine hydrase, creatine kinase, third
Or mixtures thereof one of pyruvate kinase, lactic dehydrogenase.
When test object is creatinine, multiple photophores of the first light source issue light wavelength include 510nm,
545nm、340nm。
Embodiment 2:
The present embodiment is detected using the device in embodiment 1, is further illustrated to the device.
The present embodiment is detected using colorimetric method.Sample cavity number in specific shade guide teeth is 9, from the sample of 1-9
The closure container of product chamber is 2 and contains respectively:
1) creatine concentration is the Standard blood sample of 700 μm of ol/L, picrate, SDS, borax mixture；
2) creatine concentration is the Standard blood sample of 0 μm of ol/L, picrate, SDS, borax mixture；
3) deionized water, picrate, SDS, borax mixture；
4) creatine concentration is the Standard blood sample of 700 μm of ol/L, sarcosine oxidase, phosphate buffer, 4- amino
Antipyrine, ascorbic acid oxidase, potassium ferrocyanide, Creatininase, kreatinase, peroxidase, Sodium azide mixture；
5) creatine concentration is the Standard blood sample of 0 μm of ol/L, sarcosine oxidase, phosphate buffer, 4- amino peace
For than woods, ascorbic acid oxidase, potassium ferrocyanide, Creatininase, kreatinase, peroxidase, Sodium azide mixture；
6) deionized water, sarcosine oxidase, phosphate buffer, 4-AA, ascorbic acid oxidase, Asia
The potassium ferricyanide, Creatininase, kreatinase, peroxidase, Sodium azide mixture；
7) creatine concentration is the Standard blood sample of 700 μm of ol/L, creatinine hydrase, creatine kinase, pyruvate kinase, cream
Acidohydrogenase；
8) creatine concentration is the Standard blood sample of 0 μm of ol/L, creatinine hydrase, creatine kinase, pyruvate kinase, lactic acid
Dehydrogenase；
9) deionized water, creatinine hydrase, creatine kinase, pyruvate kinase, lactic dehydrogenase.
First light source has 9 photophores, is small semiconductor laser, and launch wavelength 1-3 is 510nm, and 4-6 is
545nm, 7-9 340nm.
The light that 9 photophores issue passes through optical waveguide back reflection to shade guide teeth, and light is emitted after passing through shade guide teeth from exit portal,
Exit portal is set as 9, and 9 exit portals install optical filter respectively, and optical filter is 510nm, 4-6 545nm through wavelength 1-3,
7-9 is 340nm.
Detection terminal is the smart phone for being equipped with detection APP software.
Utilize the image of exit portal when smart phone acquisition testing.The image of exit portal is carried out automatically using cell phone application
Image segmentation is divided into standard items image and product to be tested image, and the standard items image and product to be tested image are converted to gray scale
Value, the gray value of standard of comparison product image and product to be tested image determine the concentration of product to be tested according to gray value ratio.
The step is specially to set A1, A2, A3, B1, B2, B3, C1, C2, C3 for loophole gray scale.Calculate separately A=A3/
(A1-A2)* 700 μmol/L、B= B3/( B1- B2)* 700 μmol/L、C= C3/( C1- C2)* 700 μmol/L。
The value for comparing ABC, taking numerical values recited in ABC is the result that intermediate numerical value is creatinine content.
Embodiment 3:
The present embodiment is detected using the device in embodiment 1, is further illustrated to the device.
The present embodiment is detected using fluorescence method, and test object is the urine sample containing Ca ion.Specific colorimetric
Sample cavity number in piece is 6, and the closure container from the sample cavity of 1-6 is 2 and contains respectively:
1) Ca ion concentration is the standard urine sample of 700 μm of ol/L, Fluo-3 AM fluorescence probe；
2) Ca ion concentration is the standard urine sample of 0 μm of ol/L, Fluo-3 AM fluorescence probe；
3) deionized water, Fluo-3 AM fluorescence probe；
4) Ca ion concentration is the standard urine sample of 700 μm of ol/L, Fluo-4 AM fluorescence probe；
5) Ca ion concentration is the standard urine sample of 0 μm of ol/L, Fluo-4 AM fluorescence probe；
6) deionized water, Fluo-4 AM fluorescence probe；
Second light source is small semiconductor laser, launch wavelength 495nm.Second horizontal optical channel is provided with 6 transflections
Mirror, by being respectively 1/6,1/5,1/4,1/3,1/2,1 apart from the proximal and distal reflectivity of second light source.
Light passes through optical waveguide back reflection to shade guide teeth, and light is emitted after passing through shade guide teeth from exit portal, and exit portal is set as 6
A, 6 exit portals install optical filter respectively.
Detection terminal is the smart phone for being equipped with detection APP software.
Utilize the image of exit portal when smart phone acquisition testing.The image of exit portal is carried out automatically using cell phone application
Image segmentation is divided into standard items image and product to be tested image, and the standard items image and product to be tested image are converted to gray scale
Value, the gray value of standard of comparison product image and product to be tested image determine the concentration of product to be tested according to gray value ratio.
The step is specially to set A1, A2, A3, B1, B2, B3 for loophole gray scale.Calculate separately A=A3/ (A1-A2) *
700 μmol/L、B= B3/( B1- B2)* 700 μmol/L。
The value for comparing AB, taking (A+B)/2 is the result of measurement.
Claims (7)
1. a kind of optical detection apparatus for detecting blood and urine sample, it is characterised in that including shade guide teeth, sample injector, detector
And detection terminal；
The shade guide teeth is layer structure, accommodates sample to be tested in layer structure；The sample injector and detector insert shade guide teeth
Enter, sample and reagent is added in comparison color chips after shade guide teeth is inserted by sample injector, and detector carries out sample after being inserted into shade guide teeth
Detection；Wherein detector is that multiple light courcess, multi-pass are arranged, selection monochromatic light source module or multiple light courcess mode when use；It is wherein single
Light source mode is used for fluorescence detection method, and multiple light courcess mode is used for delustring colorimetric determination；The detection terminal acquisition testing figure
Picture calculates gray value of image and obtains the concentration of sample to be tested；
The shade guide teeth is made of trilaminate material, respectively transparent underlayer, comb shape interlayer, transparent overlay, the transparent underlayer and
Transparent overlay is transparent fused silica glass material, and comb shape sandwich material is PMMA, and whole is in comb shape；Comb shape interlayer one end
With comb shape tooth, there is the sample cavity for accommodating sample when detecting between comb shape tooth；It adopts the inlet that sample cavity is connect with the external world
It is sealed with container is blocked；The closure container is made of thin-film material, inside accommodates the reagent or standard with example reaction
The standard sample of concentration；The closure container of the inlet setting of the sample cavity is one or two.
2. detection device according to claim 1, it is characterised in that:
The sample injector appearance is a cuboid, and top is equipped with the addition pool that sample to be tested is added, and side wall is offered shade guide teeth
The sample injector socket of insertion sets sample injector channel in sample injector socket, and sample injector channel and horizontal plane have angle obliquely,
Sample injector channel end is connected to addition pool, and sample-adding bottom of pond portion is set as downward tapered hole, and the tapered hole is used in shade guide teeth
The closure container is punctured after insertion sample injector socket, so as to make sample while the sample cavity of shade guide teeth is connected to addition pool
Sample cavity is flowed into the reagent blocked in container.
3. detection device according to claim 2, it is characterised in that:
The detector appearance is a cuboid, and side wall is equipped with the detector socket that is inserted into shade guide teeth, in detector socket
If detector channel, detector channel and horizontal plane have angle obliquely；
Another side wall opposite with detector socket is provided with first light source socket, the first light source socket on the detector
It is inserted into first light source, the first light source is made of multiple photophores arranged side by side, and the wavelength of multiple photophores is different；
It is provided with first level optical channel in first light source socket, multiple optical waveguides, optical waveguide count are provided in first level optical channel
The light level propagation identical as photophore quantity, optical waveguide is used to issue photophore is measured, is independent of each other；First level optical channel
End is located at below detector channel, and first level optical channel end is provided with semi-transparent semi-reflecting lens and vertical optical channel, vertical light
Channel passes through detector channel, and semi-transparent semi-reflecting lens for reflecting the light that the first photophore issues straight up, the first photophore
The light of sending is emitted after passing through sample cavity from the exit portal of detector upper surface.
4. detection device according to claim 3, it is characterised in that:
Second light source socket is provided on the detector on the side wall vertical with first level optical channel, second light source socket is inserted
Enter second light source, the second light source is single wavelength light source；Second horizontal optical channel is set in second light source socket, and second is horizontal
Optical channel is vertical with first level optical channel；The level height of second horizontal optical channel is lower than first level optical channel, the second water
Zero diopter channel end is located at immediately below vertical optical channel and is connected to vertical optical channel, and the second horizon light channel end is provided with N
A transflection mirror, the reflectivity of the transflection mirror farthest apart from second light source are 1, and the reflectivity of penultimate transflection mirror is 1/2, with
This analogizes, and the reflectivity of the transflection mirror nearest apart from second light source is 1/N；N number of transflection mirror is used for the light N for issuing second light source
It is reflected straight up after equal part, the light that the second photophore issues is emitted after passing through sample cavity from the exit portal of detector upper surface；
The exit portal is set as multiple, each exit portal is inserted into individual optical filter.
5. detection device according to claim 4, it is characterised in that:
The detection terminal setting has image collecting device and image processing apparatus, and image acquisition device is emitted when detecting
Mouth image, image processing apparatus carry out image segmentation to exit portal image, standard items image and product to be tested image are divided into, by institute
It states standard items image and product to be tested image is converted to gray value, the gray value of standard of comparison product image and product to be tested image, according to
Gray value ratio determines the concentration of product to be tested.
6. according to the described in any item detection devices of claim 3-5, it is characterised in that: described to block the reagent stored in container
Include: creatine concentration is standard urine sample, the creatinine that Standard blood sample, the creatine concentration of 700 μm of ol/L is 700 μm of ol/L
Concentration is standard urine sample, picrate, SDS, the boron that Standard blood sample, the creatine concentration of 0 μm of ol/L is 0 μm of ol/L
Sand, sarcosine oxidase, phosphate buffer, 4-AA, ascorbic acid oxidase, potassium ferrocyanide, Creatininase,
One of kreatinase, peroxidase, Sodium azide, creatinine hydrase, creatine kinase, pyruvate kinase, lactic dehydrogenase or it is mixed
Close object.
7. according to the described in any item detection devices of claim 3-5, which is characterized in that described when test object is creatinine
The wavelength that multiple photophores of first light source issue light includes 510nm, 545nm, 340nm.
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| CN109567827B (en) * | 2018-11-20 | 2019-12-13 | 陈欣 | clinical laboratory's intelligence indicates blood inspection device |
| CN111562237A (en) * | 2020-05-26 | 2020-08-21 | 中国科学院合肥物质科学研究院 | CO based on double-beam cavity enhanced spectroscopy technology2、N2O stable isotope simultaneous detection device and method |
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| CN205229055U (en) * | 2015-12-15 | 2016-05-11 | 重庆理工大学 | Urine mummification analytical equipment based on many monochromatic light and optic fibre |
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| CN107091804A (en) * | 2017-04-25 | 2017-08-25 | 天津众科创谱科技有限公司 | A kind of multi parameter analysis colorimetric measuring device |
| CN107389674A (en) * | 2017-07-28 | 2017-11-24 | 康泰医学系统（秦皇岛）股份有限公司 | A kind of urinalysis device |
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