CN107970143A - Application of the Fusarium oxysporum A654 extracts in skin-lightening cosmetic is prepared - Google Patents
Application of the Fusarium oxysporum A654 extracts in skin-lightening cosmetic is prepared Download PDFInfo
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- CN107970143A CN107970143A CN201711447118.9A CN201711447118A CN107970143A CN 107970143 A CN107970143 A CN 107970143A CN 201711447118 A CN201711447118 A CN 201711447118A CN 107970143 A CN107970143 A CN 107970143A
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- Prior art keywords
- fusarium oxysporum
- fermentation
- extracts
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- tyrosinase
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Animal Behavior & Ethology (AREA)
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- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
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- Birds (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of application of Fusarium oxysporum A654 extracts in skin-lightening cosmetic is prepared.The Fusarium oxysporum A654 extracts of the present invention are prepared by the following method：Using Fusarium oxysporum Fusarium oxysporum A654 as fermentation strain, liquid fermentation obtains fermentation culture medium, separates mycelium and zymotic fluid, takes zymotic fluid to be extracted with ethyl acetate, up to Fusarium oxysporum A654 extracts after extract is concentrated.The present invention is found through experiments that, the IC that Fusarium oxysporum A654 extracts suppress tyrosinase50It is worth for 160.35 μ g/mL, it is suitable with vitamin C to tyrosinase inhibition.Therefore, Fusarium oxysporum A654 extracts of the invention have obvious inhibiting effect to tyrosinase, available for tyrosinase inhibitor is prepared, applied to skin-lightening cosmetic.
Description
Technical field：
The invention belongs to Chinese medicine, natural cosmetics field, and in particular to prepared by a kind of Fusarium oxysporum A654 extracts
Application in skin-lightening cosmetic.
Background technology：
Plant endogenesis epiphyte has abundant species diversity and genetic diversity, is sent out from plant endogenesis epiphyte so far
Many new or rare fungal species are showed, it has also become research hotspot both domestic and external.Research is found, plants what endogenetic fungus produced
Reactive compound can produce various structure types uniquenesses, with before potential application far beyond the scope of its plant metabolites
The active material of scape, it has also become find the valuable source of new natural active matter.
The colour of skin of human skin is related with the type and quantity of pigment present in skin, and wherein melanocyte is to skin color shadow
Sound is maximum, and the tyrosine in melanocyte generates DOPA under the action of tyrosinase, DOPA quinone ultimately forms melanocyte.Tyrosine
Enzyme is a kind of metalloenzyme for being widely present in cupric in animal and plant body and microorganism, is the key enzyme of organism synthesis melanocyte, it
The forming process of melanocyte is controlled, its level of activity plays a major role the deposition of pigment, can suppress by suppressing its activity
The generation of melanin, thus tyrosinase inhibitor is widely used in cosmetics and pharmaceuticals industry as skin whitener.Mesh
The preceding whitening agent both at home and abroad for cosmetics is mainly quinhydrones, kojic acid, tartaric acid, ursin and Vc derivatives etc., but due to these
Whitening agent is there are the shortcomings of cytotoxicity is strong, percutaneous permeability is weak and stability is poor or expensive, therefore, from natural plants
Safe and effective tyrosinase inhibitor is found in extract has become the heat of insider's research applied to skin-lightening cosmetic
Point.
Fusarium oxysporum Fusarium oxysporum according to the present invention are separated from medicinal plant Pogostemon cablin stem
Obtain.Fusarium oxysporum Fusarium oxysporum A654 are disclosed in document " Pogostemon cablin endogenetic fungus Analysis on Groups and its anti-
Bacterium activity research " (Wang Ying etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2017 (4)：657-662), the Fusarium oxysporum is also reported in text
Fusarium oxysporum A654 extracts are to bacillus subtilis Bacillus subtilis, Peronophythora Litchii
Peronophthora litchic have obvious inhibitory action；Document " grind by the bioactivity of medicinal plant Pogostemon cablin endogenetic fungus
Study carefully " (horse is operated, China National folk medicine, and 2017, (13)：28-32) report Fusarium oxysporum Fusarium
Oxysporum A654 extracts have tumour cell SF-268, MCF-7, NCI-H460, HepG-2 certain cytotoxic activity,
And the research in terms of suppressing tyrosinase activity in relation to Fusarium oxysporum Fusarium oxysporum A654 extracts does not appear in the newspapers
Road.
The content of the invention：
The object of the present invention is to provide application of the Fusarium oxysporum A654 extracts in skin-lightening cosmetic is prepared.
First purpose of the present invention is to provide Fusarium oxysporum Fusarium oxysporum A654 extracts and is preparing
Application in tyrosinase inhibitor, the Fusarium oxysporum Fusarium oxysporum A654 extracts be by with
Prepared by lower section method：Fermented using Fusarium oxysporum Fusarium oxysporum A654 as fermentation strain, liquid fermentation
Culture, separates mycelium and zymotic fluid, takes zymotic fluid to be extracted with ethyl acetate, up to Fusarium oxysporum after extract is concentrated
Fusarium oxysporum A654 extracts.
It is preferred that it is by Fusarium oxysporum Fusarium oxysporum that the liquid fermentation, which obtains fermentation culture medium,
A654 is inoculated into fermentation medium, in 28 DEG C, 120r/min liquid fermentation and cultures, obtains fermentation culture medium, the fermentation
Every liter of culture medium is to be prepared by the following method：200g potato 20min are boiled with 500mL pure water, juice is filtered to obtain, adds grape
Sugared 20g, KH2PO4 3g、MgSO40.75g, vitamin B110mg, complements to 1L, pH is naturally, obtain fermentation medium with water.
The tyrosinase inhibitor is preferably the skin-lightening cosmetic for having to tyrosinase activity inhibitory action.
Second object of the present invention is to provide a kind of skin-lightening cosmetic for suppressing tyrosinase, which includes
A effective amount of Fusarium oxysporum Fusarium oxysporum A654 extracts are as active ingredient, the Fusarium oxysporum
Fusarium oxysporum A654 extracts are prepared by the following method：With Fusarium oxysporum Fusarium
Oxysporum A654 obtain fermentation culture medium as fermentation strain, liquid fermentation, separate mycelium and zymotic fluid, take zymotic fluid
It is extracted with ethyl acetate, up to Fusarium oxysporum Fusarium oxysporum A654 extracts after extract is concentrated.
It is preferred that it is by Fusarium oxysporum Fusarium oxysporum that the liquid fermentation, which obtains fermentation culture medium,
A654 is inoculated into fermentation medium, in 28 DEG C, 120r/min liquid fermentation and cultures, obtains fermentation culture medium, the fermentation
Every liter of culture medium is to be prepared by the following method：200g potato 20min are boiled with 500mL pure water, juice is filtered to obtain, adds grape
Sugared 20g, KH2PO4 3g、MgSO40.75g, vitamin B110mg, complements to 1L, pH is naturally, obtain fermentation medium with water.
The present invention is found through experiments that, the IC that Fusarium oxysporum A654 extracts suppress tyrosinase50It is worth for 160.35 μ
G/mL, the IC that positive control medicine vitamin C suppresses tyrosinase50It is worth and is extracted for 153.13 μ g/mL, Fusarium oxysporum A654
Thing is suitable with vitamin C to tyrosinase inhibition.Therefore, Fusarium oxysporum A654 extracts of the invention have obvious suppression
Tyrosinase processed effect, available for preparing skin-lightening cosmetic.
The Fusarium oxysporum Fusarium oxysporum A654 of the present invention are separated from medicinal plant Pogostemon cablin stem
Arrive, it is disclosed in document：Wang Ying etc., Pogostemon cablin endogenetic fungus Analysis on Groups and its antibacterial activity research CHINA JOURNAL OF CHINESE MATERIA MEDICAs,
2017(4)：657-662 (i.e. strains A 654) and horse are operated, in the bioactivity research of medicinal plant Pogostemon cablin endogenetic fungus
Nationality of state folk medicine, 2017, (13)：28-32, bacterial strain the present inventor also hold, and ensure from the applying date in 20 years to public affairs
Crowd provides.
Embodiment：
Below by way of specific embodiment, the present invention is further described, but not limits the present invention.
The preparation of Fusarium oxysporum Fusarium oxysporum A654 extracts：
Fusarium oxysporum Fusarium oxysporum A654 are inoculated into fermentation medium, in 28 DEG C, 120r/min
Liquid fermentation and culture, obtains fermentation culture medium, and filtering, separates mycelium and zymotic fluid, take zymotic fluid to be extracted with ethyl acetate, and extracts
Up to Fusarium oxysporum A654 extracts after taking liquid concentrated, in following embodiments.
Every liter of fermentation medium is to be prepared by the following method：200g potato 20min are boiled with 500mL pure water, are filtered
Juice, adds glucose 20g, KH2PO4 3g、MgSO40.75g, vitamin B110mg, 1L is complemented to water, and pH is naturally, obtain
To fermentation medium.
Embodiment 1：Fusarium oxysporum A654 extracts suppress tyrosinase activity measure
The preparation of pH6.8, the phosphate buffer solution (PBS buffer) that concentration is 0.05moL/L：Weigh 0.3549g
Na2HPO450mL is settled to distilled water；Weigh 0.3402g KH2PO450mL is settled to distilled water.It is by concentration
The Na of 0.05moL/L2HPO4Solution and 0.05moL/L KH2PO4Equivalent solution mixes, and obtains pH6.8, concentration 0.05moL/L
Phosphate buffer solution (PBS buffer).
The preparation of Fusarium oxysporum A654 extract solutions：Fusarium oxysporum A654 extracts are dissolved and prepared with DMSO
Into 100mg/mL concentration, the prepare liquid of 20mg/mL concentration is diluted to PBS buffer.
The preparation of tyrosinase solution：The tyrosinase 6.8mg that unit of activity is 3933U/mg is weighed, adds PBS bufferings
Liquid 2.67mL, is made into 10KU/mL concentration.
The preparation of L-dopa substrate solutions：L-dopa 33.5mg are weighed, PBS buffer 17mL is added, is configured to
10mmoL/L concentration.
By above-mentioned Fusarium oxysporum A654 extracts prepare liquid with PBS buffer be diluted to 600 μ g/mL, 400 μ g/mL,
200 μ g/mL, 100 μ g/mL, 80 μ g/mL, 60 μ g/mL, tyrosinase solution are diluted to 10U/mL concentration with PBS buffer, with
The L-dopa solution of 10mmoL/L concentration is substrate.Draw 160 μ L concentration be 10mmoL/L L-dopa solution in 96 orifice plates,
Add 20 μ L Fusarium oxysporums A654 extracts dilutions and 20 μ L-Tyr enzyme liquid dilutions make sample sets, with PBS buffer
Make reference, sample background group is made instead of tyrosinase dilution with PBS buffer, Fusarium oxysporum is replaced with PBS buffer
A654 extract dilutions make negative control group, replace Fusarium oxysporum A654 extracts dilution to make with vitamin C positive right
According to group, 37 DEG C of incubator arrest reaction 30min are put, measure the light absorption value (OD) at per hole 475nm with microplate reader, experiment repeats 3
It is secondary, inhibiting rate of the Fusarium oxysporum A654 extracts to tyrosinase activity is calculated as follows, using SigmaPolot
10.0 softwares calculate IC50Value.
Inhibiting rate (%)={ 1- (ODSample sets or positive controls/negative control group-ODBackground group)/ODNegative control group} × 100%
The result shows that：The IC that Fusarium oxysporum A654 extracts suppress tyrosinase50It is worth for 160.35 μ g/mL, the positive
The IC that control drug vitamin C suppresses tyrosinase50It is worth for 153.13 μ g/mL, Fusarium oxysporum A654 extracts are to junket ammonia
Sour enzyme inhibition is suitable with vitamin C.Therefore, Fusarium oxysporum A654 extracts of the invention have tyrosinase obvious
Inhibitory action, available for tyrosinase inhibitor is prepared, applied to skin-lightening cosmetic.
Claims (5)
1. application of the Fusarium oxysporum Fusarium oxysporum A654 extracts in tyrosinase inhibitor is prepared, institute
The Fusarium oxysporum Fusarium oxysporum A654 extracts stated are prepared by the following method：With Fusarium oxysporum
Fusarium oxysporum A654 obtain fermentation culture medium as fermentation strain, liquid fermentation, separate mycelium and fermentation
Liquid, takes zymotic fluid to be extracted with ethyl acetate, up to Fusarium oxysporum Fusarium oxysporum A654 after extract is concentrated
Extract.
2. application according to claim 1, it is characterised in that it is by sharp spore that the liquid fermentation, which obtains fermentation culture medium,
Sickle-like bacteria Fusarium oxysporum A654 are inoculated into fermentation medium, in 28 DEG C, 120r/min liquid fermentation and cultures,
Fermentation culture medium is obtained, every liter of the fermentation medium is to be prepared by the following method：200g potatos are boiled with 500mL pure water
20min, filters to obtain juice, adds glucose 20g, KH2PO4 3g、MgSO40.75g, vitamin B110mg, is complemented to water
1L, pH are naturally, obtain fermentation medium.
3. application according to claim 1 or 2, it is characterised in that the tyrosinase inhibitor is to tyrosinase
Activity has the skin-lightening cosmetic of inhibitory action.
4. a kind of skin-lightening cosmetic for suppressing tyrosinase, it is characterised in that the skin-lightening cosmetic includes a effective amount of sharp spore sickle
Knife bacterium Fusarium oxysporum A654 extracts are as active ingredient, the Fusarium oxysporum Fusarium
Oxysporum A654 extracts are prepared by the following method：With Fusarium oxysporum Fusarium oxysporum A654
As fermentation strain, liquid fermentation obtains fermentation culture medium, separates mycelium and zymotic fluid, takes zymotic fluid to be extracted with ethyl acetate
Take, up to Fusarium oxysporum Fusarium oxysporum A654 extracts after extract is concentrated.
5. the skin-lightening cosmetic according to claim 4 for suppressing tyrosinase, it is characterised in that the liquid fermentation obtains
Fermentation culture medium be that Fusarium oxysporum Fusarium oxysporum A654 are inoculated into fermentation medium, in 28 DEG C,
120r/min liquid fermentation and cultures, obtain fermentation culture medium, every liter of the fermentation medium is to be prepared by the following method：With
500mL pure water boils 200g potato 20min, filters to obtain juice, adds glucose 20g, KH2PO4 3g、MgSO40.75g, dimension life
Plain B110mg, complements to 1L, pH is naturally, obtain fermentation medium with water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711447118.9A CN107970143B (en) | 2017-12-27 | 2017-12-27 | Application of fusarium oxysporum A654 extract in preparing whitening cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711447118.9A CN107970143B (en) | 2017-12-27 | 2017-12-27 | Application of fusarium oxysporum A654 extract in preparing whitening cosmetics |
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| CN107970143A true CN107970143A (en) | 2018-05-01 |
| CN107970143B CN107970143B (en) | 2020-11-20 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101037704A (en) * | 2006-12-30 | 2007-09-19 | 福建省农业科学院生物技术研究所 | Detecting method for fusarium oxysporum pathogenicless strain |
| CN102755354A (en) * | 2012-03-13 | 2012-10-31 | 李绍平 | Fusarium oxysporum extract and application thereof |
| CN104203936A (en) * | 2011-12-11 | 2014-12-10 | 威尔金制药有限公司 | Metalloenzyme inhibitor compounds |
| CN104498372A (en) * | 2015-01-06 | 2015-04-08 | 邵阳学院 | Fusarium oxysporum BM201, compound pectinase produced by fusarium oxysporum BM201 and preparation method and application of compound pectinase |
-
2017
- 2017-12-27 CN CN201711447118.9A patent/CN107970143B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101037704A (en) * | 2006-12-30 | 2007-09-19 | 福建省农业科学院生物技术研究所 | Detecting method for fusarium oxysporum pathogenicless strain |
| CN104203936A (en) * | 2011-12-11 | 2014-12-10 | 威尔金制药有限公司 | Metalloenzyme inhibitor compounds |
| CN102755354A (en) * | 2012-03-13 | 2012-10-31 | 李绍平 | Fusarium oxysporum extract and application thereof |
| CN104498372A (en) * | 2015-01-06 | 2015-04-08 | 邵阳学院 | Fusarium oxysporum BM201, compound pectinase produced by fusarium oxysporum BM201 and preparation method and application of compound pectinase |
Non-Patent Citations (3)
| Title |
|---|
| 李雯珊等: "白木香内生真菌Fusarium sp.A2固体发酵产物的化学成分研究", 《广东药学院学报》 * |
| 王昌涛: "《化妆品植物添加剂的开发与应用》", 30 April 2013 * |
| 王营等: "广藿香内生真菌类群分析及其抗菌活性研究", 《中国中药杂志》 * |
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