CN107966567A - A kind of haptoglobin assay kit - Google Patents
A kind of haptoglobin assay kit Download PDFInfo
- Publication number
- CN107966567A CN107966567A CN201711161909.5A CN201711161909A CN107966567A CN 107966567 A CN107966567 A CN 107966567A CN 201711161909 A CN201711161909 A CN 201711161909A CN 107966567 A CN107966567 A CN 107966567A
- Authority
- CN
- China
- Prior art keywords
- reagent
- haptoglobin
- formula
- bsa
- assay kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to biological technical field, and in particular to Protein Assay Kit, is more particularly to a kind of haptoglobin measure reagent.The haptoglobin assay kit stated includes the reagent 1 and reagent 2 of independent packaging.The formula of mentioned reagent 1 includes：50 150mM TRIS buffers, 3 12g/L polyoxyethylene laurel ethers 35,3 8g/L bovine serum albumin(BSA)s, 4 8g/L sodium chloride and 0.1 0.3mL/L liquid bio preservatives.Reinforcing agent is further included in mentioned reagent 1 and reagent 2, after adding reinforcing agent, the stability of reagent greatly improves.
Description
Technical field
The invention belongs to biological technical field, and in particular to Protein Assay Kit, is more particularly to a kind of tactile pearl egg
White measure reagent.
Background technology
Haptoglobin is also known as hoptoglobin, is a kind of acute phase protein, is synthesized in liver cell, is by two light chains (α
Chain) and two heavy chains (β chains) composition glycoprotein.Haptoglobin can the free hemoglobin with reference to caused by haemolysis, and be transported to
Liver cell is degraded.When haemolysis occurs, since its removing speed haptoglobin speed newer than liver synthesis is faster, so
Any situation that erythroclasis can be made to cause hemoglobin to increase, all can reduce haptoglobin concentration in serum.Hemolytic
Concentration significantly reduces when anaemia, hepatocellular damage；Concentration is significantly raised when inflammation, wound and coronary heart disease.Therefore haptoglobin
Measure has important biology and medical significance.Haptoglobin antibody can produce agglutinating reaction, shape with the haptoglobin in serum
Into antigen antibody complex, its turbidity height is directly proportional to haptoglobin in serum in the presence of a certain amount of antibody.Pass through measure
The absorbance of specific wavelength, the concentration of haptoglobin in serum can be calculated with reference to calibration curve.
In one aspect, appear on the market at present or disclosed haptoglobin detection reagent or the species of kit and few.One side
Face make it that the scope that user can select is narrow；On the other hand, the product that appears on the market means that market competition is small less, and price is high,
And then customer using cost remains high；Another aspect, also so that this type product be difficult to it is competing by active market
Strive to form effective technology promotion, so that the type product does not have new development technically for a long time.
In another aspect, just at present from the point of view of published haptoglobin measure product, although product category is insufficient, its
Serious technical problem has been exposed in practical applications.For example, State Food and Drug Administration is in November, 2012
Issue within 26th：Ortho-Clinical Diagnostics, Inc companies are to haptoglobin assay kit (immunoturbidimetry)
Recalled, due to user using when frequently occur sample treatment and the problem of measurement result can not be obtained, it is given birth to by the said firm
Haptoglobin assay kit (immunoturbidimetry) (number of registration of the part model batch of production：State's food medicine prison tool (into) word 2010
No. 2402877) actively recall.Although specifically recalling without reference to Chinese market, but still tactile pearl at present is reflected
There are certain technological deficiency and unstability for protein assay reagent (box).
The country of China also has some haptoglobins measure product to appear on the market at present.Such as Zhejiang Kangte Biotech Co., Ltd.
(commercially available 1) or Ningbo Ruiyuan Biotechnology Co., Ltd. (the haptoglobin assay kit (immunoturbidimetry) of commercially available 2) production,
Both kits are for the content of haptoglobin in Quantitative in vitro detection human serum.The product condition of storage of both kits
And the term of validity is：(drying, lucifuge) stores under the conditions of 2-8 DEG C, the term of validity 12 months.Through actual verification, both kits
After unlatching, reagent therein is kept in dark place at 2-8 DEG C at most to be stablized 30 days, and calibration object therein is kept in dark place most at 2-8 DEG C
Stablize 7 days more.Have commercially available haptoglobin measure product the sealing term of validity even only 6 months less than.And a product from
Dispatch from the factory, to undergoing dealers at different levels, then to hospital or other use mechanism, many situations of circulation or haulage time undergone
The several months long are up to down.That is mentioned reagent box its actual term of validity when reaching in user's hand has greatly shortened,
Along with reagent is once opened only a couple of days its stationary phase, most tens of days.Above reason can cause postmature delivery before largely Kaifeng
Fail product after product or Kaifeng, these expired or failure products should be discarded or recycled in theory, but one actually therein
Divide and be still mingled in circulating pathway.This aspect causes the significant wastage of resource, on the other hand causes the confusion in market, more
Notably these expired failure products are likely to cause the measurement result of mistake so as to cause serious medical thing once being used for tester
Therefore.
Although technical staff can not participate in the management of commodity circulation, however can technological layer reduce as far as possible
State the possibility of situation generation.Therefore, it should be the neck to improve the stability of haptoglobin measure product, simplify its preservation condition
Research staff endeavours an important directions of research.
The newest technology innovation that can be retrieved at present is a kind of disclosed in Chinese patent application 201510407469 touches
Globin assay kit, but the emphasis of the invention is the mechanical realization of kit, it disclose only haptoglobin inspection
Part conventional reagent in survey, such as polyethylene glycol, phosphate buffer, sheep anti-human haptoglobin antibody, bovine serum albumin(BSA)
Deng.And any new approaches are not provided to the stability for how improving detection kit.
In conclusion how to further improve the stability of haptoglobin measure reagent (box), sensitivity, accuracy
It can be the research direction that this area merits attention.
The content of the invention
To make up the deficiencies in the prior art, the present invention provides a kind of haptoglobin assay kit.With prior art phase
The performances such as accuracy, sensitivity than, the kit are improved to some extent, while the stability of the kit obtains significantly
Improve, its preservation condition is simplified.
The technical solution adopted by the present invention is：
A kind of haptoglobin assay kit, it is characterised in that：The haptoglobin assay kit includes independent wrap
The reagent 1 and reagent 2 of dress.
The formula of mentioned reagent 1 includes：50-150mM TRIS buffers, the 3-12g/L polyoxyethylene moons
Osmanthus ether -35,3-8g/L bovine serum albumin(BSA)s, 4-8g/L sodium chloride and 0.1-0.3mL/L liquid bio preservatives.
Preferably, the formula of mentioned reagent 1 includes：70-130mM TRIS buffers, 5-11g/L polyoxies
Ethene bay ether -35,4-7g/L bovine serum albumin(BSA)s, 5-7g/L sodium chloride and 0.1-0.3mL/L liquid bio preservatives.
It is further preferred that the formula of mentioned reagent 1 includes：80-110mM TRIS buffers, 7-
10g/L polyoxyethylene laurel ethers -35,4-6g/L bovine serum albumin(BSA)s, 5-6g/L sodium chloride and 0.1-0.3mL/L liquid bios are prevented
Rotten agent.
In a preferred embodiment, the formula of mentioned reagent 1 includes：100mM trishydroxymethylaminomethanes buffer
Liquid, 10g/L polyoxyethylene laurel ethers -35,5g/L bovine serum albumin(BSA)s, 5.85g/L sodium chloride and 0.2mL/L liquid bio anti-corrosions
Agent.
In another preferred embodiment, the formula of mentioned reagent 1 includes：90mM trishydroxymethylaminomethanes buffer
Liquid, 8.5g/L polyoxyethylene laurel ethers -35,4.5g/L bovine serum albumin(BSA)s, 5.35g/L sodium chloride and 0.1mL/L liquid bios
Preservative.
The formula of mentioned reagent 2 includes：50-150mM TRIS buffers, 10-30mL/L goat-anti people touch
Globin antibodies, 3-8g/L bovine serum albumin(BSA)s and 0.1-0.3mL/L liquid bio preservatives.
Preferably, the formula of mentioned reagent 2 includes：70-130mM TRIS buffers, 15-25mL/L sheep
Anti-human haptoglobin antibody, 4-7g/L bovine serum albumin(BSA)s and 0.1-0.3mL/L liquid bio preservatives.
It is further preferred that the formula of mentioned reagent 2 includes：80-110mM TRIS buffers, 18-
20mL/L sheep anti-human haptoglobin antibody, 4-6g/L bovine serum albumin(BSA)s and 0.1-0.3mL/L liquid bio preservatives.
In a preferred embodiment, the formula of mentioned reagent 2 includes：100mM trishydroxymethylaminomethanes buffer
Liquid, 20mL/L sheep anti-human haptoglobin antibody, 5g/L bovine serum albumin(BSA)s and 0.2mL/L liquid bio preservatives.
In another preferred embodiment, the formula of mentioned reagent 2 includes：80mM trishydroxymethylaminomethanes buffer
Liquid, 20mL/L sheep anti-human haptoglobin antibody, 4g/L bovine serum albumin(BSA)s and 0.2mL/L liquid bio preservatives.
The liquid bio preservative arrived involved in the present invention can be selected from ProClin150, ProClin200 or
Any one in ProClin300；It preferably is selected from any one in ProClin200 or ProClin300.
Outside it is expected that, after reinforcing agent is added in mentioned reagent 1 or reagent 2, not only so that provided by the invention touch
The detection accuracy of globin assay kit and sensitivity slightly improve, it is often more important that substantially increase its stability, make
Kit preservation condition is obtained to simplify.
Above-mentioned reinforcing agent can be carbohydrate；The carbohydrate is trisaccharide；The trisaccharide is preferably gossypose or its derivative
Thing.
Gossypose (Raffinose) be widely present with nature, it is fragrant such as in the vegetables such as beet, potato, cabbage
In the fruit such as any of several broadleaf plants, grape, the cereal such as rice, wheat is medium.Gossypose is combined into by galactolipin, fructose and glucose, is
A kind of functional oligose.
Gossypose has effects that to adjust or strengthen immunity, moisturizing beauty, adjust the function such as blood pressure or, therefore extensively should
Field for food, medicine, cosmetics etc..
Present invention discovery also unexpected in implementation process, when gossypose be present in certain content range it is above-mentioned
When in reagent 1 or reagent 2, relatively more preferable enhancing effect can be played, this content range is 0.5-3g/L, is preferably 0.7-
1.9g/L, more preferably 0.9-1.3g/L；When its content is less than 0.5g/L, do not observe to reagent 1 or reagent 2
Have obvious enhancing effect, thus it is speculated that it is a kind of possible the reason for be when its content is less than 0.5g/L, gossypose in reagent dividing
Quantum count is not enough to form effective intermolecular contacts, so that effective enhancing effect can not be played；When its content is more than 3g/L
When, have obvious enhancing effect to reagent 1 or reagent 2 also without observing, thus it is speculated that it is a kind of possible the reason for be when its content
During more than 3g/L, its molecular amounts in reagent is excessive, influences other formula (such as liquid bio preservative) molecules and exists
Interaction in reagent, so as to can not also play effective enhancing effect.
It may occur to persons skilled in the art that other have the trisaccharide of same or similar molecular structure should with gossypose
For can also play above-mentioned technique effect in the present invention, therefore should be contained within the scope of protection of present invention.
Therefore, as a preferred embodiment, haptoglobin assay kit of the present invention includes independent wrap
The reagent 1 and reagent 2 of dress；The formula of the reagent 1 includes：50-150mM TRIS buffers, 3-12g/L
Polyoxyethylene laurel ether -35,3-8g/L bovine serum albumin(BSA)s, 4-8g/L sodium chloride, 0.1-0.3mL/L liquid bios preservative and
0.5-3g/L gossyposes；The formula of the reagent 2 includes：50-150mM TRIS buffers, 10-30mL/L
Sheep anti-human haptoglobin antibody, 3-8g/L bovine serum albumin(BSA)s, 0.1-0.3mL/L liquid bios preservative and 0.5-3g/L cotton seeds
Sugar.
Further, haptoglobin assay kit provided by the invention further includes calibration object, and the calibration object includes
Independent packaging loads in mixture haptoglobin, sodium chloride, bovine serum albumin(BSA), sucrose and liquid biological preservative together.
Therefore, include independent as another preferred embodiment, haptoglobin assay kit of the present invention
Reagent 1, reagent 2 and the calibration object of packaging；The formula of the reagent 1 includes：50-150mM trishydroxymethylaminomethanes buffer
Liquid, 3-12g/L polyoxyethylene laurel ethers -35,3-8g/L bovine serum albumin(BSA)s, 4-8g/L sodium chloride, the life of 0.1-0.3mL/L liquid
Thing preservative and 0.5-3g/L gossyposes；The formula of the reagent 2 includes：50-150mM trishydroxymethylaminomethanes buffer
Liquid, 10-30mL/L sheep anti-human haptoglobin antibody, 3-8g/L bovine serum albumin(BSA)s, 0.1-0.3mL/L liquid bios preservative and
0.5-3g/L gossyposes；Haptoglobin, sodium chloride, the ox blood that the calibration object includes independent packaging or loads in mixture together are pure
Albumen, sucrose and liquid biological preservative.
The application method of haptoglobin assay kit provided by the invention is preferably：
(1) basic parameter：
1) reaction type：End-point method；2) reaction temperature：37℃；3) dominant wavelength：340nm；4) secondary wavelength：700nm；5) sample
This amount：3μl；6) reagent 1 (R1) is measured：240μl；7) reagent 2 (R2) is measured：60μl；8) the Direction of Reaction：Rise reaction；
(2) step：Reagent 1 is added into sample, reaction measures absorbance and is calculated as A1 after five minutes；Reagent 2 is added,
Reaction measures absorbance and is calculated as A2 after five minutes；Absorbance change value is calculated as follows：△ A=A2-A1；With the suction of calibration object
Light varience value △ A are ordinate, its corresponding concentration C draws calibration curve for abscissa, bent in calibration according to the △ A of sample
Haptoglobin concentration in corresponding sample is found out on line.
In above-mentioned application method, the type of sample is serum, is preferably Diagnostic Value of Fasting Serum.
All formula ranges arrived involved in the present invention include all integers and decimal within the range, such as 3-12g/
L polyoxyethylene laurel ethers -35 include all decimal g/L polyoxyethylene moons between 3,4,5,6,7,8,9,10,11,12 or 3-12
Osmanthus ether -35.
Embodiment
The explanation of following embodiments is only intended to help the method and its core concept for understanding the present invention.It should be pointed out that pair
For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out
Some improvement and modification, these are improved and modification is also fallen into the protection domain of the claims in the present invention.To disclosed implementation
The description below of example, enables professional and technical personnel in the field to realize or use the present invention.A variety of modifications to these embodiments
It will be apparent for those skilled in the art, the general principles defined herein can not depart from this
In the case of the spirit or scope of invention, realize in other embodiments.Therefore, the present invention is not intended to be limited to illustrated herein
These embodiments in, but can be applied to meet the broader model consistent with the principles and novel features disclosed herein
Enclose.
Unless otherwise defined, all technical and scientific terms used herein have and the technical field of the invention
The normally understood identical meaning of those of ordinary skill.
Such as it is not particularly illustrated, the material (including organic substance and inorganic substances) involved in the present invention is to pass through business
Approach obtains.
A kind of haptoglobin assay kit of embodiment 1
Reagent 1, reagent 2 and calibration object comprising independent packaging.
Wherein, calibration object include the haptoglobin of independent packaging, sodium chloride, bovine serum albumin(BSA), sucrose and
Proclin300。
Wherein, the formula of reagent 1 and reagent 2 is as shown in the table：
A kind of haptoglobin assay kit of embodiment 2
Reagent 1, reagent 2 and calibration object comprising independent packaging.
Wherein, calibration object include the haptoglobin of independent packaging, sodium chloride, bovine serum albumin(BSA), sucrose and
Proclin300。
Wherein, the formula of reagent 1 and reagent 2 is as shown in the table：
A kind of haptoglobin assay kit of embodiment 3
Reagent 1, reagent 2 and calibration object comprising independent packaging.
Wherein, calibration object include the haptoglobin of independent packaging, sodium chloride, bovine serum albumin(BSA), sucrose and
Proclin300。
Wherein, the formula of reagent 1 and reagent 2 is as shown in the table：
Such as be not particularly illustrated, involved in embodiment of the present invention to embodiment or comparative example formula in, addition
The mother liquid concentration of sheep anti-human haptoglobin antibody is 20mg/mL.
A kind of haptoglobin assay kit of embodiment 4
The difference of the present embodiment and embodiment 3 is：0.5g/ is further included in the reagent 1 of the present embodiment and the formula of reagent 2
The gossypose of L, remaining is the same as embodiment 3.
A kind of haptoglobin assay kit of embodiment 5
The difference of the present embodiment and embodiment 3 is：3g/L is further included in the reagent 1 of the present embodiment and the formula of reagent 2
Gossypose, remaining is the same as embodiment 3.
A kind of haptoglobin assay kit of embodiment 6
The difference of the present embodiment and embodiment 3 is：1g/L is further included in the reagent 1 of the present embodiment and the formula of reagent 2
Gossypose, remaining is the same as embodiment 3.
A kind of haptoglobin assay kit of embodiment 7
The difference of the present embodiment and embodiment 6 is：By in the reagent 1, reagent 2 and calibration object of embodiment 6
Proclin300 replaces with Proclin200, remaining is the same as embodiment 6.
A kind of haptoglobin assay kit of embodiment 8
The difference of the present embodiment and embodiment 6 is：By in the reagent 1, reagent 2 and calibration object of embodiment 6
Proclin300 replaces with Proclin150, remaining is the same as embodiment 6.
A kind of haptoglobin assay kit of embodiment 9
The difference of the present embodiment and embodiment 6 is：By the trihydroxy methyl amino first in the reagent 1 and reagent 2 of embodiment 6
The concentration of alkane buffer solution is changed to 70mM, remaining is the same as embodiment 6.
A kind of haptoglobin assay kit of comparative example 1
The difference of the present embodiment and embodiment 6 is：Gossypose is dense in the reagent 1 of the present embodiment and the formula of reagent 2
Spend for 0.4g/L, remaining is the same as embodiment 6.
A kind of haptoglobin assay kit of comparative example 2
The difference of the present embodiment and embodiment 6 is：Gossypose is dense in the reagent 1 of the present embodiment and the formula of reagent 2
Spend for 3.5g/L, remaining is the same as embodiment 6.
Performance test：Make a service test to the reagent 1 in above-described embodiment and comparative example and reagent 2
1st, the range of linearity：Utilize haptoglobin pattern detection above-described embodiment of concentration known and the linear model of comparative example
Enclose, it is as a result as follows：
| Example | The range of linearity | Linearly dependent coefficient (r) |
| Embodiment 1 | 0.5-2.7g/L | ≥0.9900 |
| Embodiment 2 | 0.3-2.8g/L | ≥0.9900 |
| Embodiment 3 | 0.2-3.0g/L | ≥0.9900 |
| Embodiment 4 | 0.1-3.0g/L | ≥0.9900 |
| Embodiment 5 | 0.2-3.3g/L | ≥0.9900 |
| Embodiment 6 | 0.05-4.0g/L | ≥0.9999 |
| Embodiment 7 | 0.2-3.5g/L | ≥0.9900 |
| Embodiment 8 | 0.1-3.4g/L | ≥0.9900 |
| Embodiment 9 | 0.2-3.5g/L | ≥0.9900 |
| Comparative example 1 | 0.1-3.5g/L | ≥0.9900 |
| Comparative example 2 | 0.1-3.0g/L | ≥0.9900 |
2nd, stability
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention god.
Claims (10)
- A kind of 1. haptoglobin assay kit, it is characterised in that：The haptoglobin assay kit includes independent packaging Reagent 1 and reagent 2；The formula of the reagent 1 includes：50-150mM TRIS buffers, 3-12g/L gather Ethylene oxide bay ether -35,3-8g/L bovine serum albumin(BSA)s, 4-8g/L sodium chloride and 0.1-0.3mL/L liquid bio preservatives；Institute The formula for the reagent 2 stated includes：50-150mM TRIS buffers, 10-30mL/L sheep anti-human haptoglobins resist Body, 3-8g/L bovine serum albumin(BSA)s and 0.1-0.3mL/L liquid bio preservatives.
- 2. haptoglobin assay kit as claimed in claim 1, it is characterised in that：The formula of the reagent 1 includes： 70-130mM TRIS buffers, 5-11g/L polyoxyethylene laurel ethers -35,4-7g/L bovine serum albumin(BSA)s, 5- 7g/L sodium chloride and 0.1-0.3mL/L liquid bio preservatives；The formula of the reagent 2 includes：70-130mM trihydroxy methyls Aminomethane buffer solution, 15-25mL/L sheep anti-human haptoglobin antibody, 4-7g/L bovine serum albumin(BSA)s and 0.1-0.3mL/L liquid Body biological preservative.
- 3. haptoglobin assay kit as claimed in claim 2, it is characterised in that：The formula of the reagent 1 includes： 80-110mM TRIS buffers, 7-10g/L polyoxyethylene laurel ethers -35,4-6g/L bovine serum albumin(BSA)s, 5- 6g/L sodium chloride and 0.1-0.3mL/L liquid bio preservatives；The formula of the reagent 2 includes：80-110mM trihydroxy methyls Aminomethane buffer solution, 18-20mL/L sheep anti-human haptoglobin antibody, 4-6g/L bovine serum albumin(BSA)s and 0.1-0.3mL/L liquid Body biological preservative.
- 4. haptoglobin assay kit as claimed in claim 3, it is characterised in that：The formula of the reagent 1 includes： 100mM TRIS buffers, 10g/L polyoxyethylene laurel ethers -35,5g/L bovine serum albumin(BSA)s, 5.85g/L chlorine Change sodium and 0.2mL/L liquid bio preservatives；The formula of the reagent 2 includes：100mM trishydroxymethylaminomethanes buffer Liquid, 20mL/L sheep anti-human haptoglobin antibody, 5g/L bovine serum albumin(BSA)s and 0.2mL/L liquid bio preservatives.
- 5. the haptoglobin assay kit as described in claim 1-4 any one, it is characterised in that：The liquid bio Any one of preservative in ProClin150, ProClin200 or ProClin300.
- 6. haptoglobin assay kit as claimed in claim 5, it is characterised in that：The liquid bio preservative is selected from Any one in ProClin200 or ProClin300.
- 7. haptoglobin assay kit as claimed in claim 6, it is characterised in that：The liquid bio preservative is ProClin300。
- 8. the haptoglobin assay kit as described in claim 1-4 any one, it is characterised in that：The reagent 1 Formula includes：50-150mM TRIS buffers, 3-12g/L polyoxyethylene laurel ethers -35,3-8g/L cow's serums Albumin, 4-8g/L sodium chloride, 0.1-0.3mL/L liquid bios preservative and 0.5-3g/L gossyposes；The reagent 2 is matched somebody with somebody Side includes：50-150mM TRIS buffers, 10-30mL/L sheep anti-human haptoglobin antibody, 3-8g/L ox bloods Pure albumen, 0.1-0.3mL/L liquid bios preservative and 0.5-3g/L gossyposes.
- 9. haptoglobin assay kit as claimed in claim 8, it is characterised in that：The formula of the reagent 1 includes： 100mM TRIS buffers, 10g/L polyoxyethylene laurel ethers -35,5g/L bovine serum albumin(BSA)s, 5.85g/L chlorine Change sodium, 0.2mL/L liquid bios preservative and 1g/L gossyposes；The formula of the reagent 2 includes：100mM trihydroxy methyl ammonia Methylmethane buffer solution, 20mL/L sheep anti-human haptoglobin antibody, 5g/L bovine serum albumin(BSA)s, 0.2mL/L liquid bio preservatives With 1g/L gossyposes.
- 10. the haptoglobin assay kit as described in claim 1-4 any one, it is characterised in that：The tactile pearl egg White assay kit includes reagent 1, reagent 2 and the calibration object of independent packaging；The formula of the reagent 1 includes：50-150mM TRIS buffer, 3-12g/L polyoxyethylene laurel ethers -35,3-8g/L bovine serum albumin(BSA)s, 4-8g/L chlorinations Sodium, 0.1-0.3mL/L liquid bios preservative and 0.5-3g/L gossyposes；The formula of the reagent 2 includes：50-150mM tri- Hydroxymethyl aminomethane buffer solution, 10-30mL/L sheep anti-human haptoglobin antibody, 3-8g/L bovine serum albumin(BSA)s, 0.1- 0.3mL/L liquid bios preservative and 0.5-3g/L gossyposes；The calibration object includes independent packaging or loads in mixture together Haptoglobin, sodium chloride, bovine serum albumin(BSA), sucrose and liquid biological preservative.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711161909.5A CN107966567B (en) | 2017-11-21 | 2017-11-21 | A kind of haptoglobin assay kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711161909.5A CN107966567B (en) | 2017-11-21 | 2017-11-21 | A kind of haptoglobin assay kit |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107966567A true CN107966567A (en) | 2018-04-27 |
| CN107966567B CN107966567B (en) | 2018-12-18 |
Family
ID=61999706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711161909.5A Active CN107966567B (en) | 2017-11-21 | 2017-11-21 | A kind of haptoglobin assay kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107966567B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116298317A (en) * | 2023-03-14 | 2023-06-23 | 浙江夸克生物科技有限公司 | Alpha fetoprotein determination kit based on latex immunoturbidimetry |
| CN117368466A (en) * | 2023-12-08 | 2024-01-09 | 安徽惠邦生物工程有限公司 | Kit for measuring urine binding globin by using biochemical analyzer and preparation method thereof |
Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52125623A (en) * | 1976-04-13 | 1977-10-21 | Chugai Pharmaceut Co Ltd | Quantitative determination of antigenic substances and reagent kit for the same |
| WO2001027631A1 (en) * | 1999-10-12 | 2001-04-19 | The University Court Of The University Of Glasgow | Assays for mastitis detecting haptoglobin in milk |
| CN101066450A (en) * | 2002-02-14 | 2007-11-07 | 中外制药株式会社 | Antibody-containing solution preparation |
| CN101368948A (en) * | 2008-09-24 | 2009-02-18 | 王贤理 | Immune globulin M detection reagent |
| WO2009086400A2 (en) * | 2007-12-28 | 2009-07-09 | Baxter International Inc. | Recombinant vwf formulations |
| CN101688170A (en) * | 2007-04-24 | 2010-03-31 | 生物马特里卡公司 | Sample storage for life science |
| CN101981450A (en) * | 2008-03-26 | 2011-02-23 | 默克专利有限公司 | Method of long term storage of substrate-coupled beads |
| WO2011058087A1 (en) * | 2009-11-11 | 2011-05-19 | Gentian As | Immunoassay for assessing related analytes of different origin |
| CN102128935A (en) * | 2010-11-23 | 2011-07-20 | 中生北控生物科技股份有限公司 | Method for detecting hoptoglobin in serum and detecting kit thereof |
| WO2012085497A1 (en) * | 2010-12-20 | 2012-06-28 | Reactivlab Limited | Assay method |
| CN103018464A (en) * | 2012-12-12 | 2013-04-03 | 元升生物科技（上海）有限公司 | Reagent for determining procalcitonin and preparation method of reagent |
| CN103562720A (en) * | 2011-03-28 | 2014-02-05 | 三菱化学美迪恩斯株式会社 | Method and kit for measuring immunity in whole blood sample |
| CN103926405A (en) * | 2014-05-08 | 2014-07-16 | 北京玖佳宜科技有限公司 | Creatine kinase isozyme detection kit and preparation thereof |
| CN104330575A (en) * | 2014-11-17 | 2015-02-04 | 中国人民解放军第三0五医院 | Troponin I detection reagent and preparation method thereof |
| CN105044358A (en) * | 2015-07-13 | 2015-11-11 | 绍兴圣康生物科技有限公司 | Haptoglobin determination kit |
| CN105067615A (en) * | 2015-08-28 | 2015-11-18 | 深圳市汇松科技发展有限公司 | Detection kit and detection method for detecting animal C-reactive protein based on amide group nanometer latex enhanced turbidimetry |
| CN105223356A (en) * | 2015-10-20 | 2016-01-06 | 常州英赞美科生物科技有限公司 | GPI latex enhancing immune turbidimetry Quantitative in vitro measures diagnostic kit |
| CN105548573A (en) * | 2016-02-02 | 2016-05-04 | 潍坊三维生物工程集团有限公司 | Kit and method for detecting content of KAPPA light chain and application |
| CN106053839A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining haptoglobin and preparation method thereof |
-
2017
- 2017-11-21 CN CN201711161909.5A patent/CN107966567B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52125623A (en) * | 1976-04-13 | 1977-10-21 | Chugai Pharmaceut Co Ltd | Quantitative determination of antigenic substances and reagent kit for the same |
| WO2001027631A1 (en) * | 1999-10-12 | 2001-04-19 | The University Court Of The University Of Glasgow | Assays for mastitis detecting haptoglobin in milk |
| CN101066450A (en) * | 2002-02-14 | 2007-11-07 | 中外制药株式会社 | Antibody-containing solution preparation |
| CN101688170A (en) * | 2007-04-24 | 2010-03-31 | 生物马特里卡公司 | Sample storage for life science |
| WO2009086400A2 (en) * | 2007-12-28 | 2009-07-09 | Baxter International Inc. | Recombinant vwf formulations |
| CN101981450A (en) * | 2008-03-26 | 2011-02-23 | 默克专利有限公司 | Method of long term storage of substrate-coupled beads |
| CN101368948A (en) * | 2008-09-24 | 2009-02-18 | 王贤理 | Immune globulin M detection reagent |
| WO2011058087A1 (en) * | 2009-11-11 | 2011-05-19 | Gentian As | Immunoassay for assessing related analytes of different origin |
| CN102128935A (en) * | 2010-11-23 | 2011-07-20 | 中生北控生物科技股份有限公司 | Method for detecting hoptoglobin in serum and detecting kit thereof |
| WO2012085497A1 (en) * | 2010-12-20 | 2012-06-28 | Reactivlab Limited | Assay method |
| CN103562720A (en) * | 2011-03-28 | 2014-02-05 | 三菱化学美迪恩斯株式会社 | Method and kit for measuring immunity in whole blood sample |
| CN103018464A (en) * | 2012-12-12 | 2013-04-03 | 元升生物科技（上海）有限公司 | Reagent for determining procalcitonin and preparation method of reagent |
| CN103926405A (en) * | 2014-05-08 | 2014-07-16 | 北京玖佳宜科技有限公司 | Creatine kinase isozyme detection kit and preparation thereof |
| CN104330575A (en) * | 2014-11-17 | 2015-02-04 | 中国人民解放军第三0五医院 | Troponin I detection reagent and preparation method thereof |
| CN105044358A (en) * | 2015-07-13 | 2015-11-11 | 绍兴圣康生物科技有限公司 | Haptoglobin determination kit |
| CN105067615A (en) * | 2015-08-28 | 2015-11-18 | 深圳市汇松科技发展有限公司 | Detection kit and detection method for detecting animal C-reactive protein based on amide group nanometer latex enhanced turbidimetry |
| CN105223356A (en) * | 2015-10-20 | 2016-01-06 | 常州英赞美科生物科技有限公司 | GPI latex enhancing immune turbidimetry Quantitative in vitro measures diagnostic kit |
| CN105548573A (en) * | 2016-02-02 | 2016-05-04 | 潍坊三维生物工程集团有限公司 | Kit and method for detecting content of KAPPA light chain and application |
| CN106053839A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining haptoglobin and preparation method thereof |
Non-Patent Citations (9)
| Title |
|---|
| C. LIPPERHEIDE等: "Nephelometric determination of haptoglobin plasma concentrations in fattening pigs", 《J. VET. MED.》 * |
| FREDERICK VAN LENTE等: "Evaluation of a nephelometric assay for haptoglobin and its clinical usefulness", 《CLINICAL CHEMISTRY》 * |
| H. J. M. VAN RIJN等: "Is the turbidimetric immunoassay of haptoglobin phenotype-dependent", 《CLINICAL BIOCHEMISTRY》 * |
| J. M. RAMAKERS等: "Turbidimetric determination of haptoglobin", 《J. CLIN. CHEM. CLIN. BIOCHEM.》 * |
| 吴惠毅等: "免疫比浊法测定血清结合珠蛋白", 《上海免疫学杂志》 * |
| 张茜: "建立定量试验分析性能和临床性能的评价方案及系统评价免疫透射比浊原理测定结合珠蛋白的定量试验", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 贺兰湘等: "国产结合珠蛋白抗血清在免疫化学分析仪上的应用", 《上海医学检验杂志》 * |
| 邹雄等: "《临床检验仪器》", 31 August 2015, 中国医药科技出版社 * |
| 韩成莲等: "比浊法测定结合珠蛋白试剂盒的性能评价", 《中国医学装备》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116298317A (en) * | 2023-03-14 | 2023-06-23 | 浙江夸克生物科技有限公司 | Alpha fetoprotein determination kit based on latex immunoturbidimetry |
| CN117368466A (en) * | 2023-12-08 | 2024-01-09 | 安徽惠邦生物工程有限公司 | Kit for measuring urine binding globin by using biochemical analyzer and preparation method thereof |
| CN117368466B (en) * | 2023-12-08 | 2024-03-12 | 安徽惠邦生物工程有限公司 | Kit for measuring urine binding globin by using biochemical analyzer and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107966567B (en) | 2018-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nougier et al. | Hypofibrinolytic state and high thrombin generation may play a major role in SARS‐COV2 associated thrombosis | |
| Robinson et al. | Erythrocytes: a new cell type for the evaluation of insulin receptor defects in diabetic humans | |
| Henke et al. | MUC5AC and MUC5B mucins are decreased in cystic fibrosis airway secretions | |
| Nagai et al. | Inhibition of AGEs formation by natural products | |
| Tamakoshi et al. | White blood cell count and risk of all-cause and cardiovascular mortality in nationwide sample of japanese results from the NIPPON DATA90 | |
| CN109613264A (en) | A kind of Apolipoprotein C2 assay kit of high sensitivity | |
| CN103076455A (en) | Kit for quickly and quantificationally detecting serum amyloid A, and preparation and application thereof | |
| CN107966567A (en) | A kind of haptoglobin assay kit | |
| El-Sayed et al. | ABO blood groups in correlation with hyperlipidemia, diabetes mellitus type II, and essential hypertension | |
| CN110646624A (en) | ELISA reagent diagnosis kit of matrin 2 and use method thereof | |
| CN108872606A (en) | A kind of highly stable, sensitive hoptoglobin（HP）Detection kit | |
| Thamer et al. | Relationship between moderate alcohol consumption and adiponectin and insulin sensitivity in a large heterogeneous population | |
| US11067579B2 (en) | Target marker GP73 for detecting steatohepatitis and detection application method | |
| CN107153121B (en) | Glycosylated hemoglobin, hemoglobin detection kit and its detection method | |
| Sone et al. | Type 2 Diabetes Prevalence in Asian Subjects: Response to McNeely and Boyko | |
| Groene et al. | Comparison of two different fibrinogen concentrates in an in vitro model of dilutional coagulopathy | |
| CN106556705B (en) | A kind of enzyme-linked immunologic detecting kit and preparation method thereof of solubility ST2 | |
| CN102964428A (en) | Kit for detecting soluble leukocyte differentiation antigen 40 ligand and polypeptide ligand | |
| Vinicor et al. | The metabolic syndrome: the emperor needs some consistent clothes: response to Davidson and Alexander | |
| CN108445218A (en) | The kit and preparation method thereof of joint-detection CRP, PCT and SAA | |
| CN105158463A (en) | Colloidal gold test paper bar for antibody detection of sheep Brucella | |
| RU2463610C1 (en) | METHOD FOR MAKING PANEL OF HBsAg SUBTYPES AD AND AY SERUMS FOR QUALITY CONTROL OF DIAGNOSING HEPATITIS B | |
| Nayeri et al. | Clinical impact of real-time evaluation of the biological activity and degradation of hepatocyte growth factor | |
| Mahmood et al. | Implication of serum and salivary albumin tests in the recent oral health related epidemiological studies: A narrative review | |
| Drzewoski et al. | Is aspirin resistance a real problem in people with type 2 diabetes? Response to Sacco et al. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |