CN107966530A - A kind of high temperature ageing method of rice paddy seed - Google Patents
A kind of high temperature ageing method of rice paddy seed Download PDFInfo
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- CN107966530A CN107966530A CN201711159696.2A CN201711159696A CN107966530A CN 107966530 A CN107966530 A CN 107966530A CN 201711159696 A CN201711159696 A CN 201711159696A CN 107966530 A CN107966530 A CN 107966530A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The present invention provides a kind of high temperature ageing method of rice paddy seed, the rice material of storage characteristics to be measured is placed in closed high temperature ageing box, place into baking oven, handled under conditions of baking oven high temperature drying after a certain period of time, measure its germination percentage and germination percentage declines percentage change, and the changing rule of percentage is declined to judge the storage endurance characteristic of rice, so as to filter out storage endurance seed material with germination percentage and germination percentage.The present invention utilizes closed high-temperature oxidation process under the conditions of high temperature drying, you can obtains the result of the test repeated with the basically identical stabilization of actual high temperature drying anaerobic storage condition.This method is quick, easy, easy, it is not necessary to expensive laboratory apparatus.Thus, seed production, storage and processing evaluation and scientific research etc. can be widely used in.
Description
Technical field
The invention belongs to crop germplasm resource field, and in particular to a kind of high temperature ageing method of rice paddy seed.
Background technology
Rice is the most important cereal crops of China, and there is more than 60% population in the whole nation using rice as staple food.Rice storage endurance
Characteristic research is the major issue for being related to national economy.Jiang Jiayue etc. (2007) discloses a kind of artificial ageing screening storage endurance crop
The method of germplasm, its principle are by under the conditions of the hot and humid artificial ageing of reference substance seed and crop germplasm to be screened progress
Artificial ageing, screening storage endurance crop germplasm (ZL200710019675.0).However, beautiful jade etc. (2010) research is found, it is new to receive
The hot and humid artificial accelerated aging test result of rice paddy seed obtained is there are significant difference and unstability, its influence factor is not only
It is and related with the growth cabinet difference number of plies, Aged Seeds quantity with temperature, humidity.
It is, in principle, that hot and humid artificial accelerated aging method be mainly seed in the case where water content is higher,
Its amylolytic enzyme and fat hydrolase etc. are activated, what the reserve substance such as starch, fat then occurred under the action of hydrolase
Hydrolysis, generates glucose and free fatty, both materials particularly the latter further oxygenolysis, then can produce
The harmful substances such as substantial amounts of free radical, cause Seed senescence dead.
The storage condition of closed high temperature drying is then different, and seed moisture content is low, then mainly happens is that high-temperature oxidation process.
Grain is in long-term real storage is hidden, and with the extension of storage time, its moisture gradually scatters and disappears, and water content gradually reduces, and
Due to the bulk deposition of grain, make between most of seed in storehouse oxygen thin or in oxygen free condition, thus summer high temperature is special
It is not that the north in China is usually more conform with the storage condition of closed high temperature drying.
The content of the invention
In order to overcome existing tropical deterioration technology and the difference of the storage condition of actual high temperature drying, the present invention provides
A kind of high temperature ageing method of rice paddy seed.
The technical solution adopted by the present invention is as follows：
A kind of high temperature ageing method of rice paddy seed, comprises the following steps：
(1) selection of seed material：
By the rice material that the growing and cultivation for treating resistant storage properties identification is consistent with harvest, maturity is consistent, it is dried to
After moisture to less than 12.00% safe moisture, remove weeds therein, dust, ghost, without shell rice and injured kernel etc., ensure
Material uniformity, with reference to the initial germination percentage of the method measure seed material of national standard GB/T3543.4-1995；
(2) high temperature ageing box is made by oneself：
Make a closed high temperature ageing box by oneself, easy to storage of the seed material in artificial accelerated aging case and anaerobic bar is set
Part；
(3) oven temperature is set：
Using the standing baking oven in laboratory, its temperature is first preheated to 50-60 DEG C；
(4) high temperature ageing is handled：
The seed material to be measured of above-mentioned selection is put into the closed aging box of self-control, then is placed in the baking oven for reaching preheating temperature
In, certain time is handled respectively, is sampled, and germination percentage is measured with reference to the method for national standard GB/T3543.4-1995；
(5) evaluation of storage characteristics：
Germination percentage after statistical disposition, calculates germination percentage and declines percentage according to the following equation：
Germination percentage declines percentage=(germination percentage of initial germination percentage-processing after a certain period of time) × 100/ and initially germinates
Rate,
Decline the changing rule of percentage according to germination percentage, judge different disposal time rice under the conditions of closed high temperature ageing
The resistant storage properties of material.
The closed high temperature ageing box is cubical aluminum box, and aging box is equipped with rectangle box lid, the both sides of box lid
Equipped with hasp, box lid can be made to play fixed and sealing function, aging box volume is 100-200 milliliters.
Further, the long 10cm of the closed high temperature ageing box, width 5cm, high 3cm.
Further, the preheating temperature of the baking oven is at 57 DEG C.
Further, when the high temperature ageing processing time is respectively 24,48,72 small.
Beneficial effects of the present invention：
The present invention utilizes closed high-temperature oxidation process under the conditions of closed high temperature drying, you can obtains and actual storage bar
The result of the test that the basically identical stabilization of part repeats, overcomes the hot and humid artificial accelerated aging of existing seed and is done with high temperature
The shortcomings that dry anaerobic storage physical condition difference, and the changing rule of percentage is declined to judge rice with germination percentage and germination percentage
Storage endurance characteristic.This method is quick, easy, easy, it is not necessary to expensive laboratory apparatus.Thus, seed can be widely used in
Production, storage and processing evaluation and scientific research etc..
Brief description of the drawings
The accompanying drawings which form a part of this application are used for providing a further understanding of the present invention, and of the invention shows
Meaning property embodiment and its explanation are used to explain the present invention, do not form inappropriate limitation of the present invention.
The block diagram of the germination percentage variation of Fig. 1 different temperatures different time processing material；
The structure diagram of Fig. 2 high temperature ageing boxes.
Embodiment
Embodiment 1
1st, the selection of seed material：
Utilize four rice varieties：Bamboo is blue or green, rich two excellent No. 4, II excellent 838, japonica rice 755.
2nd, process is tested：
By above-mentioned rice varieties bamboo it is blue or green, rich two excellent No. 4, II excellent 838,755 growing and cultivation of japonica rice and harvest it is consistent, into
After the consistent harvest of ripe degree, after being dried to moisture to less than 12.00% safe moisture, and weeds therein, dust, sky are removed
Shell, without shell rice and injured kernel etc., ensure material uniformity, with reference to national standard GB/T3543.4-1995 method measure just originate
Bud rate；
Using the closed aging box of homemade aluminum, as shown in Figure 1, then by each 100g of four kinds of test materials to be measured be put into from
In aging box processed, oven temperature is respectively set to 50 DEG C, 55 DEG C, 57 DEG C, 60 DEG C, then aging box is placed in and has adjusted temperature
In baking oven, when processing 24,48,72 is small, periodically sampling, germination percentage is measured with reference to the method for national standard GB/T3543.4-1995.
Germination percentage after statistical disposition, calculates germination percentage and declines percentage according to the following equation：
Germination percentage declines percentage=(germination percentage of initial germination percentage-processing after a certain period of time) × 100/ and initially germinates
Rate.
The results are shown in Table 1 for statistics：
The germination percentage of 1 rice material different temperatures different time of table processing declines percentage (%)
3rd, test results and analysis：
As shown in figure 1 and table 1, it is known that：During 50 DEG C of processing, except 755 germination percentage of japonica rice and germination percentage decline percentage have it is aobvious
Write and become outside the pale of civilization, bamboo is blue or green, rich two excellent No. 4, II excellent 838 material process 24,48,72 it is small when, germination percentage and germination percentage decline percentage
Change unobvious, thus be difficult to differentiate between the resistant storage properties of this 3 materials；
During 55 DEG C of processing, bamboo green grass or young crops storage endurance, 7,550 points of round-grained rice not storage endurance, but rich two excellent No. 4, II excellent 838 materials can be distinguished
When handling 24,72 hours, germination percentage decline percentage is almost identical, only variant when 48 is small, thus is difficult to differentiate between its resistance to storage
Hide sex differernce；
During 60 DEG C of processing, in addition to bamboo is blue or green, other materials germination percentage is reduced to zero, thus is also difficult to differentiate between；And 57 DEG C of processing
Then different, 24 rich two excellent No. 4 germination percentages when small decline percentage 34.04%, can be substantially distinguished from bamboo green grass or young crops and II excellent 838,48 is small
When II excellent 838 material germination percentage decline percentage 43.70%, can be substantially distinguished from bamboo green grass or young crops, 72 it is small when bamboo green grass or young crops germination percentage decline hundred
It is still 15.24 to divide rate, then is substantially distinguished from other 3 materials, has preferable resistant storage properties.
4th, conclusion：57 DEG C are the suitable treatment temperatures of rice high temperature ageing method.
Claims (5)
1. a kind of high temperature ageing method of rice paddy seed, it is characterised in that comprise the following steps：
（1）The selection of seed material：
By the rice material that the growing and cultivation for treating resistant storage properties identification is consistent with harvest, maturity is consistent, moisture is dried to
To less than 12.00% safe moisture, remove weeds therein, dust, ghost, without shell rice and injured kernel etc., ensure that material is equal
It is even consistent, with reference to the initial germination percentage of the method measure seed material of national standard GB/T3543.4-1995；
（2）Make high temperature ageing box by oneself：
Make a closed high temperature ageing box by oneself, easy to storage of the seed material in artificial accelerated aging case and oxygen free condition is set；
（3）Oven temperature is set：
Using the standing baking oven in laboratory, its temperature is first preheated to 50-60 DEG C；
（4）High temperature ageing processing：
The seed material to be measured of above-mentioned selection is put into the closed aging box of self-control, then is placed in the baking oven for reaching preheating temperature,
Certain time is handled respectively, is sampled, and germination percentage is measured with reference to the method for national standard GB/T3543.4-1995；
（5）The evaluation of storage characteristics：
Germination percentage after statistical disposition, calculates germination percentage and declines percentage according to the following equation：
Germination percentage declines percentage=initial germination percentage in (germination percentage of initial germination percentage-processing after a certain period of time) × 100/,
Decline the changing rule of percentage according to germination percentage, judge different disposal time rice material under the conditions of closed high temperature ageing
Resistant storage properties.
2. the high temperature ageing method of a kind of rice paddy seed according to claim 1, it is characterised in that the closed high temperature is old
Change box is cubical aluminum box, and aging box is equipped with rectangle box lid, and the both sides of box lid are equipped with hasp, can play box lid solid
Fixed and sealing function, aging box volume are 100-200 milliliters.
3. the high temperature ageing method of a kind of rice paddy seed according to claim 2, it is characterised in that the closed high temperature is old
Change the long 10cm of box, width 5cm, high 3cm.
A kind of 4. high temperature ageing method of rice paddy seed according to claim 1, it is characterised in that the preheating of the baking oven
Temperature is at 57 DEG C.
5. the high temperature ageing method of a kind of rice paddy seed according to claim 1, it is characterised in that at the high temperature ageing
When the reason time is respectively 24,48,72 small.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109192244A (en) * | 2018-09-07 | 2019-01-11 | 福建省农业科学院水稻研究所 | A kind of fast appraisement method of rice storage endurance characteristic |
| CN111492745A (en) * | 2020-04-08 | 2020-08-07 | 吉林省农业科学院 | Method for detecting corn storage tolerance |
| CN112098451A (en) * | 2020-09-16 | 2020-12-18 | 沈阳农业大学 | Method for detecting activity of rice seeds based on low-field nuclear magnetic resonance technology |
| CN112098450A (en) * | 2020-09-16 | 2020-12-18 | 沈阳农业大学 | Method for detecting influence of physical and chemical characteristics in rice seed aging process |
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| CN101011025A (en) * | 2007-01-29 | 2007-08-08 | 中国科学院等离子体物理研究所 | Artificial burn-in screen method for preservation endurable crop |
| CN101273680A (en) * | 2008-05-06 | 2008-10-01 | 中国农业大学 | Method of Kentucky bluegrass seeds accelerated aging test |
| CN105409520A (en) * | 2015-11-26 | 2016-03-23 | 安徽省农业科学院水稻研究所 | Evaluation and authentication method for storage resistance characteristic of rice variety or germ plasm resources |
| CN106358504A (en) * | 2016-09-05 | 2017-02-01 | 山东省农作物种质资源中心 | Artificial aging method for simulating soybean seed natural aging |
| CN106385864A (en) * | 2016-09-05 | 2017-02-15 | 山东省农作物种质资源中心 | Artificial aging method for simulation of natural aging of wild soybean seeds |
-
2017
- 2017-11-20 CN CN201711159696.2A patent/CN107966530A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011025A (en) * | 2007-01-29 | 2007-08-08 | 中国科学院等离子体物理研究所 | Artificial burn-in screen method for preservation endurable crop |
| CN101273680A (en) * | 2008-05-06 | 2008-10-01 | 中国农业大学 | Method of Kentucky bluegrass seeds accelerated aging test |
| CN105409520A (en) * | 2015-11-26 | 2016-03-23 | 安徽省农业科学院水稻研究所 | Evaluation and authentication method for storage resistance characteristic of rice variety or germ plasm resources |
| CN106358504A (en) * | 2016-09-05 | 2017-02-01 | 山东省农作物种质资源中心 | Artificial aging method for simulating soybean seed natural aging |
| CN106385864A (en) * | 2016-09-05 | 2017-02-15 | 山东省农作物种质资源中心 | Artificial aging method for simulation of natural aging of wild soybean seeds |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109192244A (en) * | 2018-09-07 | 2019-01-11 | 福建省农业科学院水稻研究所 | A kind of fast appraisement method of rice storage endurance characteristic |
| CN111492745A (en) * | 2020-04-08 | 2020-08-07 | 吉林省农业科学院 | Method for detecting corn storage tolerance |
| CN112098451A (en) * | 2020-09-16 | 2020-12-18 | 沈阳农业大学 | Method for detecting activity of rice seeds based on low-field nuclear magnetic resonance technology |
| CN112098450A (en) * | 2020-09-16 | 2020-12-18 | 沈阳农业大学 | Method for detecting influence of physical and chemical characteristics in rice seed aging process |
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