CN107963916A - The purposes of endogenetic fungal bacterial strain NYN8C05 - Google Patents
The purposes of endogenetic fungal bacterial strain NYN8C05 Download PDFInfo
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- CN107963916A CN107963916A CN201711240063.4A CN201711240063A CN107963916A CN 107963916 A CN107963916 A CN 107963916A CN 201711240063 A CN201711240063 A CN 201711240063A CN 107963916 A CN107963916 A CN 107963916A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
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- Pest Control & Pesticides (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of purposes of endogenetic fungus root cup fungi bacterial strain Rhizopycnis sp.NYN8C05 in terms of soil nutrient is promoted, belong to microbe application field.Specifically, the invention discloses a kind of purposes of endogenetic fungal bacterial strain NYN8C05：For increasing the soil organism；It can increase the content of water-soluble zwitterion in soil；It can also promote rice absorbing nutrition, so as to promote paddy growth.
Description
Technical field
The present invention relates to a kind of endogenetic fungus root cup fungi bacterial strain Rhizopycnis sp.NYN8C05 to promote soil nutrient
The purposes of aspect, belongs to microbe application field.
Background technology
Endogenetic fungus (endophytic fungi) is that the part of at least history of life can be infected and colonized in health plant group
In knitting, host without obvious illness a kind of fungi (Petrini, 1991；Wilson, 1995), plant is existed only in mycorrhizal fungi
Thing root system is different, endogenetic fungus can exist in any histoorgan of the underground and aboveground of plant (Faeth＆Fagan,
2002).Endogenetic fungus is widely present in plant tissue, free from the influence of the external environment, to growth and development of plants, resistance disease pest
Evil etc. has extensive biological action, while the extensive use to some plants and crops is of great significance.With ring
Border problem it is continuous worsening, plant resources undergos acid test, and influence of the endogenetic fungus to plant be it is many,
Through as one of hot issue studied outside Now Domestic.In agricultural production, ecological protection, natural resources of Chinese medicinal materials exploitation, medical and health
Development have important realistic meaning.In recent years, it has been found that existing beneficial endogenetic bacteria and Nei Sheng in plant
Fungi, the preventive and therapeutic effect of endogenous strains on plant disease and biological development and application have caused the extensive concern of scientist.Plant
Endogenetic fungus is distributed mainly in seed, flower, stem, leaf and the root system of plant, and the relation with plant is mutualistic symbiosis, Er Qiezhi
Thing endogenetic fungus can provide photosynthate and mineral matter, and endogenous fungus metabolite can stimulate the growth of host plant to send out
Educate, the biology for improving host plant stress be with abiotic stress resistance ability, i.e., there are the circulation of material and energy between the two
Exchange, plant endogenesis epiphyte are partly the activity secreted by plant endogenesis epiphyte to the energetically growgh promoting effects of host plant
The result of material or secondary metabolite to the growth of plant.It is that root nodule homobium and mycorhiza are total to study at present more
The homobium of raw body, endogenetic fungus and plant is another form of expression of higher plant and microbe symbiotic relation.
Number of patent application is the invention of CN201510518527.8《Endogenetic fungal bacterial strain NYN8C05 and application thereof》Provide
A kind of fungal bacterial strain, the bacterial strain are endogenetic fungal bacterial strain (Rhizopycnis sp.) NYN8C05, deposit number：CCTCC NO:M
2015219.The purposes of the fungal bacterial strain is：Reduce cadmium, arsenic, the content of lead in tobacco leaf.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of new application of endogenetic fungal bacterial strain NYN8C05.
In order to solve the above technical problem, the present invention provides a kind of purposes of endogenetic fungal bacterial strain NYN8C05：For increasing
The soil organism.
Improvement as the purposes of the endogenetic fungal bacterial strain NYN8C05 of the present invention：Increase water-soluble zwitterion in soil
Content.
The further of purposes as the endogenetic fungal bacterial strain NYN8C05 of the present invention is improved：
The water soluble anion is
The water-soluble cationic is
The further of purposes as the endogenetic fungal bacterial strain NYN8C05 of the present invention is improved：Promote rice absorbing nutrition, from
And promote paddy growth.
The endogenetic fungal bacterial strain NYN8C05 of the present invention, i.e. endogenetic fungus root cup fungi bacterial strain Rhizopycnis
Sp.NYN8C05, its occupation mode are：The endogenetic fungus microbial inoculum NYN8C05 of about 5kg is added in the soil of each mu of area.
Using increasing by 21.99% soil organism after 20 days, endogenetic fungus NYN8C05 makes endogenetic fungus NYN8C05
Increase by 31.62% soil organism with after 40 days.
The endogenetic fungus NYN8C05 of the present invention can promote the increase of water soluble ion in soil, and promote soil organic
The increase of matter, increase soil fertility (that is, improves soil quality, improve soil nutrient), and then promotes rice absorbing nutrition, promotes
Paddy growth；Its popularization and application in agriculture field is of great value.
Embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Plant variety in following case：Rice (Oryza sativa) show water 134, commercially available (agriculture section of Zhejiang Province kind industry is limited
Company).
Remarks explanation：Following all culture mediums are used at the sterilizing for being preceding required to carry out high temperature and pressure in a conventional manner
Reason (121 DEG C, 0.24MPa, 20 minutes).
The effect of embodiment 1, endogenetic fungal bacterial strain Rhizopycnis sp.NYN8C05：
1st, the preparation of Rhizopycnis sp.NYN8C05 microbial inoculums
First, the Rhizopycnis sp.NYN8C05 bacterium being stored in the toothpick picking of sterilizing in Normal Atmospheric Temperature Liquid paraffin
Silk, is inoculated on PDA culture medium tablet, sealed membrane sealing, and it is spare to be positioned over the dark 25 DEG C of cultures of constant incubator.Then cultivate
Primary seed solution culture, 10 pieces of the bacteria cake beaten with the card punch of diameter 5cm is inoculated in 200ml PDB culture mediums, placement is shaken
Bed 150r/min, 25 DEG C of culture 3d.Then secondary seed solution culture is prepared, by cultured first order seed nutrient solution blender
Smash and shake up, draw 20ml and be inoculated in new PDB culture mediums 200ml, place shaking table 150r/min, 25 DEG C of culture 3d.Microbial inoculum
Matrix formulations：Wheat bran 550g, beancake powder 100g, rice bran 150g, corn particle 200g, calcium sulfate 10g, peptone 13g, KH2PO4
1.5g, MgSO40.5g, NaNO34g, sucrose 13g, add 500ml pure water, uniformly mixing, by microbial inoculum after above-mentioned material mixing
Matrix is dispensed into heat safe bacterium bag, every bag of 100g, and 121 DEG C of sterilizing 40min, after overnight, go out again under similarity condition after sealing
Once, cool down spare.Every bag of microbial inoculum matrix adds secondary seed nutrient solution 20ml, is placed in the dark static training of 25 DEG C of constant incubators
Support, cultivate 15-20 days until Rhizopycnis sp.NYN8C05 are covered with microbial inoculum matrix.
2nd, seedling cultivation of rice and potted plant experiment
Choose completely, without rice grain 1% hypochlorite disinfectant 10min, the aseptic water washing 6-7 of any scab
Secondary to no sodium hypochlorite, is put into culture dish, is sprouted in 37 DEG C of incubators, and seed can transplant when showing money or valuables one carries unintentionally (about 3 days).Transplanting
Container is the plastic tub of 20 centimetres of diameter.Cultivation matrix is according to peat：Vermiculite：Perlite=4：2：1 ratio is mixed thoroughly.Weigh life
The endogenetic fungus microbial inoculum (obtained by step 1) grown, is mixed by each potted plant 10g microbial inoculums, 150g cultivation matrixes and inserts seedling-growing container.Educate
Seedling basin, which is put into the pallet for fill with water, to absorb water, and after moisture is inhaled thoroughly, moves into each 15 of the vegetable seeds of sprouting.In this, as experiment
Group.
Also it is respectively provided with CK groups, BLANK groups；
CK (inactivation microbial inoculum) is to substitute microbial inoculum to inactivate microbial inoculum (endogenetic fungus microbial inoculum is handled through autoclave sterilization), remaining
Same experimental group；
BLANK (blank control) is to cancel microbial inoculum, there was only the treatment group of cultivation matrix.
Rhizopycnis sp.NYN8C05 microbial inoculums (experimental group), CK, BLANK distinguish 3 repetitions, paddy growth 50d days
After measure index of correlation.It is specific as follows：
(1), plant height and the measurement of root long tape measure or ruler；The increment of plant electronics balance measurement, plant water
Rinse well, aerial part is separated with ground portion, weighs fresh weight, after put 65 DEG C of baking ovens and dry to constant weight, weigh dry weight.
The biomass of table 1, rice under different endogenetic fungus microbial inoculums
Note：Blank is blank control
After endogenetic fungus NYN8C05 processing, the biomass of rice each several part all increased, wherein plant height increase
6.97%, root long increase by 3.85%, aerial part fresh weight increase by 12.55%, aerial part dry weight increase by 6.73%, under ground portion
Fresh weight increase by 1.21%, under ground portion dry weight adds 16.67%.
3rd, soil nutrient measures
The root soil of paddy growth 20d, 30d and 40d in Example one respectively.
The content of organic matter measures：Dichromate titration (Bao Shidan, 2000).Weigh by 0.149mm (100 mesh) sieve aperture
Air-dried soil sample 0.1-1G (is accurate to 0.0001g), is put into the hard test tube of a drying and is accurately added with pipette
0.8000mol·L-1(1/6K2Cr2O7) standard solution 5ml is (if need to first plus Ag containing chloride in soil2SO40.1g), use
Syringe adds dense H2SO45ml, fully shakes up, the curved small funnel of neck in mouthpiece cover, to condense the steam steamed.By 8~10 examinations
Pipe is put into temperature automatically controlled aluminium block tube socket (invisible spectro liquid temperature control is at about 170 DEG C), or 8~10 test tubes are contained in iron wire
(there is 1~2 blank cuvette in per cage) in cage, be put into the paraffin oil bath pan that temperature is 185~190 DEG C, it is desirable to after being put into
Oil bath pan temperature drops to 170~180 DEG C or so, must control electric furnace later, make oil bath kettle temperature maintain 170 all the time~
180 DEG C, start timing when liquid boiling gassing in test tube, boil 5 minutes, take out test tube and (use bath oiling, slightly cold, wiping
Fluid outside net test tube).After cooling, tube contents are poured into 250ml triangular flasks, test tube interior and small leakage is eluted with water
Struggle against, overall solution volume should keep 1/2H in mixed liquor in 60-70ml in this triangular flask2SO4) concentration is 2~3molL-1, so
Add 2- carboxyls afterwards to drip for diphenylamines indicator 12~15, solution is in brownish red at this time.With the 0.2molL of standard-1Sulfuric acid is sub-
Ferrimetry, content is constantly shaken in titration process, until the color of solution is changed into dark green (grey blue-green) from reddish brown through purple,
As titration end-point.Such as use o-phenanthrolin indicator, add indicator 2-3 drops, in the colourshifting process of solution by it is orange → bluish-green →
Brick-red is terminal.Keep FeSO in mind4Titrate ml numbers (V).Per a collection of (in i.e. above-mentioned every wire mesh cage or aluminium block) sample measure
Meanwhile carry out 2~3 blank tests, that is, take 0.500g powdered silicas to replace soil sample, other formalities and Specimen Determination phase
Together.Keep FeSO in mind4Titrate mL numbers (V0), take its average value.
Soil organic matter (gkg-1)=(c × 5/V0)×(V0-V)×10-3× 3.0 × 1.1/ formula of (m × k) × 1000
In：C=0.8000molL-1(1/6K2Cr2O7) standard solution concentration；The volume that 5=potassium bichromates standard solution adds, it is single
Position mL；V0=blank titrations spend FeSO4Volume, mL；The titration of V=samples spends FeSO4Volume, mL；3.0=1/4 carbon atoms
Molal weight, gmol-1；10-3=mL is scaled L；1.1=oxidation adjusting coefficient；M=air-dries quality of soil sample, g；K=will
Wind desiceted soil is converted into the coefficient of drying soil.The soil organism (gkg-1)=soil organic matter (gkg-1× 1.724) in formula：
1.724 change the average conversion coefficient of the soil organism into for soil organic matter.
Table 2, the lower soil with organic matter content of endogenetic fungus Rhizopycnis sp.NYN8C05 processing
As the important indicator for weighing soil fertility, the soil organism is the important component of soil, is the life of plant
The activity of long and edaphon and soil animal provides nutrition.The content of the soil organism affects the sustainable development of soil
Exhibition.In the ecosystem, plant fixes the carbon dioxide in air by photosynthesis, is stored in plant, has part then
Transport plant root.Organic matter into soil mainly exists in the form of three kinds：The plant and animal residues not decomposed by plant, it is right
It is then Leaf Litter or root residue for plant；Decomposed by edaphon, organic matter decomposition into it is simple
Inorganic compound；The colloidal substance to be formed is remerged by microorganism decomposition, can be combined closely with soil mineral particulate, this
It is that organic carbon is primarily present form in soil.Therefore, edaphon also plays during Organic Matter Transformation and focuses on
Act on, be the executor of its conversion.As shown in table 1：The content of organic matter is higher than control in the paddy soil of endogenetic fungus processing,
NYN8C05 processing 20d the content of organic matter, with NYN8C05CK (inactivation microbial inoculum) compared with increase 2.18g/kg (18.14%),
Increase 3.70g/kg (21.99%) compared with BLANK.The content of the organic matter of NYN8C05 processing is with the rice cultivating time
The trend risen is presented in passage, high in the content of organic matter of 40d, is 14.90g/kg, compared with NYN8C05CK (inactivation microbial inoculum)
Increase 2.65g/kg (21.63%), increase 3.70g/kg (31.62%) compared with BLANK.C contained by the soil organism is soil
Earth organic carbon, about 81% participates in the organic carbon storage of carbon cycle in the soil.Soil Microbial Biomass Carbon be then organic carbon instruction because
Son.There is multiple-microorganism in soil, endogenetic fungus NYN8C05 can improve the content of organic matter in paddy soil, in soil carbon sequestration
During whether play an important role, in farmland ecosystem, using endogenetic fungus NYN8C05 and crop relation, improve solid
Carbon acts on, and reduces carbon dioxide content in air, to solving the problems, such as greenhouse effects, is of great significance, in addition, endogenetic fungus
The addition of NYN8C05 can improve organic carbon content in soil environment, while reduce carbon in soil mineralising, to harmful microorganism
Produce certain inhibitory action.
4th, the water-soluble zwitterion content of the root soil of paddy growth 20d and 40d, soil ratio 1 are measured:1 extraction, from
The heart takes supernatant to cross column, ion chromatograph measure (anion：DIONEX ICS-2000；Cation：DIONEX ICS-2000).
Water-soluble zwitterion content in table 3, the lower soil of endogenetic fungus Rhizopycnis sp.NYN8C05 processing
According to table 3, can learn：By 20 days paddy growths, the soil of endogenetic fungus processing, in its soilDisappear
Blank control BLANK is consumed,【Blank(40d)-Blank(20d)】The ratio of/Blank (20d) is 20%,【NYN8C05(40d)-
NYN8C05(20d)】The ratio of/NYN8C05 (20d) illustrates that endogenetic fungus can be soil for 63%, NYN8C05 CK (20d)
In component be dissociated into the available zwitterion of rice, and promote absorption of the plant to its zwitterion.
The endogenetic fungus NYN8C05 of the present invention is promoting paddy growth, promotes rice absorbing nutrition, it is especially water-soluble from
Son, and promote the increase of the soil organism, increase soil fertility, aspect has significant outstanding feature, is led as in agricultural
Popularization and application in domain are of great value.
Finally, it should also be noted that it is listed above be only the present invention several specific embodiments.Obviously, this hair
It is bright to be not limited to above example, there can also be many deformations.Those of ordinary skill in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (4)
1. the purposes of endogenetic fungal bacterial strain NYN8C05, it is characterized in that：For increasing the soil organism.
2. the purposes of endogenetic fungal bacterial strain NYN8C05 according to claim 1, it is characterized in that：Increase water-soluble in soil
The content of zwitterion.
3. the purposes of endogenetic fungal bacterial strain NYN8C05 according to claim 2, it is characterized in that：
The water soluble anion is NO3 -、PO4 3-；
The water-soluble cationic is NH4 +、K+。
4. according to the purposes of any endogenetic fungal bacterial strain NYN8C05 of claims 1 to 3, it is characterized in that：Rice is promoted to inhale
Nutrition is received, so as to promote paddy growth.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112852643A (en) * | 2021-02-24 | 2021-05-28 | 贵州医科大学 | Application of paris polyphylla endophytic fungi in preparation of antitumor drugs |
| CN115287194A (en) * | 2022-01-30 | 2022-11-04 | 浙江中医药大学 | Medicinal wild rice endophytic fungus YYA21 and application thereof |
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| US8975489B2 (en) * | 2010-12-02 | 2015-03-10 | The Samuel Roberts Noble Foundation | Grass fungal endophytes and uses thereof |
| CN105316241A (en) * | 2015-08-21 | 2016-02-10 | 浙江中烟工业有限责任公司 | Rhizopycnis sp. NYN8C05 and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112852643A (en) * | 2021-02-24 | 2021-05-28 | 贵州医科大学 | Application of paris polyphylla endophytic fungi in preparation of antitumor drugs |
| CN115287194A (en) * | 2022-01-30 | 2022-11-04 | 浙江中医药大学 | Medicinal wild rice endophytic fungus YYA21 and application thereof |
| CN115287194B (en) * | 2022-01-30 | 2023-07-14 | 浙江中医药大学 | Medicinal wild rice endophytic fungi YYA21 and application thereof |
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