CN107962770A - A kind of biological 3D printing system based on Coulter principle - Google Patents
A kind of biological 3D printing system based on Coulter principle Download PDFInfo
- Publication number
- CN107962770A CN107962770A CN201711369736.6A CN201711369736A CN107962770A CN 107962770 A CN107962770 A CN 107962770A CN 201711369736 A CN201711369736 A CN 201711369736A CN 107962770 A CN107962770 A CN 107962770A
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- nozzle
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- particle
- printing system
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y40/00—Auxiliary operations or equipment, e.g. for material handling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y50/00—Data acquisition or data processing for additive manufacturing
- B33Y50/02—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
Abstract
The invention belongs to 3D printing technique field; biological 3D printing system more particularly to based on Coulter principle; including nozzle, perturbation mechanism, testing agency, sorting mechanism, accumulation platform and motion, wherein nozzle is used to spray droplet-like or cylindrical particle mixed liquor；Perturbation mechanism is used to disturb particle mixed liquor, makes the period profile of the particle mixed liquor that nozzle sprays in the time and spatially；Testing agency is used to detect the particle in the particle mixed liquor of nozzle ejection；Sorting mechanism is used for the particle information detected according to testing agency, by satisfactory drop into line displacement, so as to obtain required drop；By sorting mechanism sub-elect satisfactory drop accumulation platform on be piled into required shape；Motion can make nozzle carry out relative motion with accumulation platform, so that particle can carry out the accumulation of setting shape according to space.The present invention can be achieved to be precisely controlled cell or other granular materials, and it is big, it can be achieved that high density printing to print flux.
Description
Technical field
The invention belongs to 3D printing technique field, more particularly to a kind of biological 3D printing system based on Coulter principle,
For stent 3D printing, cell 3D printing, tissue 3D printing or organ 3D printing.
Background technology
Biological 3D printing technique is a kind of new technology with reference to 3D printing technique and Biotechnology, at present biometric print
Technology focuses primarily upon the Method of printing of extruded type, and for this method when printing cell, the shearing force major injury that cell is subject to is big, nothing
Method is precisely controlled cell arrangement, and can not realize the high density printing of cell.And actual biologic-organ is how complicated fine,
Extensive style Method of printing will certainly be unworkable in the development of Biotechnology.Therefore, being badly in need of one kind at present can realize pair
Cell is precisely controlled, and can realize the printing technique and equipment of high throughput 3D printing.
The content of the invention
In view of the above-mentioned problems, it is an object of the invention to provide a kind of biological 3D printing system based on Coulter principle,
Cell is precisely controlled to realize, and can realize high throughput 3D printing.
To achieve these goals, the present invention uses following technical scheme：
A kind of biological 3D printing system based on Coulter principle, including nozzle, perturbation mechanism, testing agency, sorting machine
Structure, accumulation platform and motion, wherein,
The nozzle is used to spray droplet-like or cylindrical particle mixed liquor；
The perturbation mechanism is arranged on the nozzle；
The testing agency is arranged at the end of the nozzle, and the testing agency is used to detect that the nozzle sprays
The particle of grain mixed liquor；
The sorting mechanism is arranged at the lower section of the testing agency, and the sorting mechanism is used for according to the testing agency
The particle information of detection, by satisfactory drop into line displacement, so as to obtain required drop；
The accumulation platform is arranged at the lower section of the sorting mechanism, is sub-elected by the sorting mechanism satisfactory
Drop is piled into required shape on the accumulation platform；
The motion can make nozzle carry out relative motion with the accumulation platform, so that particle can be according to space
Carry out the accumulation of setting shape.
The testing agency includes first electrode, second electrode and is arranged between the first electrode and second electrode
Insulator, the first electrode are an integral structure with the nozzle, and the first electrode is drawn with second electrode by conducting wire
Detect interface.
The nozzle is equipped with runner, and the upper end of the runner is connected with sample inlet, and lower end is connected with nozzle.
The perturbation mechanism is piezoelectric ceramics, and the piezoelectric ceramics is embedded at the annular groove that the nozzle outer surface is equipped with
It is interior.
The sorting mechanism includes the charging electrode and deflecting electrode from top to bottom set, and the charging electrode is used for liquid
Drop charges, and the deflecting electrode is used for electrically charged drop into horizontal deflection.
The charging electrode is loop configuration.
The motion is triaxial movement platform, and the triaxial movement platform connects with the accumulation platform or the nozzle
Connect.
The biological 3D printing system based on Coulter principle, further includes and is arranged at returning below the deflecting electrode
Receiving apparatus, the retracting device are used to recycle undesirable drop after the sorting mechanism sorts.
The retracting device includes accumulator tank and recovery pump, and the recovery pump is used to recycle the mixing in the accumulator tank
Liquid.
Advantages of the present invention and beneficial effect are：The accurate control to cell or other granular materials can be achieved in the present invention
System, and it is big, it can be achieved that high density printing to print flux.
Brief description of the drawings
Fig. 1 is the structure diagram of the present invention；
Fig. 2 is the structure diagram of testing agency in the present invention.
In figure：1 is nozzle, and 2 be sample inlet, and 3 be perturbation mechanism, and 4 be testing agency, and 5 be detection interface, and 6 be nozzle,
7 be charging electrode, and 8 be deflecting electrode cathode, and 9 be deflecting electrode anode, and 10 be accumulation platform, and 11 be retracting device, and 12 be the
One electrode, 13 be insulator, and 14 be second electrode.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the accompanying drawings with specific embodiment pair
The present invention is described in detail.
As shown in Figure 1, a kind of biological 3D printing system based on Coulter principle provided by the invention, including nozzle 1, disturb
Motivation structure 3, testing agency 4, sorting mechanism, accumulation platform 10 and motion, wherein nozzle 1 are used to spray droplet-like or column
Particle mixed liquor；Perturbation mechanism 3 is arranged on nozzle 1, and perturbation mechanism 3 is used to disturb particle mixed liquor, for spraying nozzle 1
Period profile of the grain mixed liquor in the time and spatially；Testing agency 4 is arranged at the end of nozzle 1, and testing agency 4 is used to detect
Whether contain particle in the particle mixed liquor that nozzle 1 sprays or contain which kind of particle；Sorting mechanism is arranged under testing agency
Side, sorting mechanism is used for the particle information detected according to testing agency, by satisfactory drop into line displacement, so as to obtain institute
The drop needed；Accumulation platform 10 is arranged at the lower section of sorting mechanism, sub-elects satisfactory drop by sorting mechanism and exists
Required shape is piled on accumulation platform 10；Motion can make nozzle 1 carry out relative motion with accumulation platform 10, so that
Particle can carry out the accumulation of setting shape according to space.
Nozzle 1 is equipped with the runner of up/down perforation, and the upper end of runner is sample inlet 2, and lower end is nozzle 6.In nozzle 1
Cavity containing particle mixed liquor, there is provided pressure entrance and nozzle, make particle mixed liquor be sprayed from nozzle 6, and droplet-like or column is presented
Shape, ultimately forms droplet-like in dropping process.
In one embodiment of the invention, perturbation mechanism 3 is piezoelectric ceramics, and piezoelectric ceramics is embedded on 1 outer surface of nozzle and sets
In some annular grooves.
As shown in Fig. 2, testing agency 4 is detected based on Coulter principle, testing agency 4 includes first electrode 12, the
Two electrodes 14 and the insulator 13 being arranged between first electrode 12 and second electrode 14, first electrode 12 are integrated with nozzle 1
Formula structure, first electrode 12 draw detection interface 5 with second electrode 14 by conducting wire.
Testing agency 4 is a kind of detection device using Coulter principle, for detecting the particle mixed liquor of the ejection of nozzle 1
In particle.
The particle information that sorting mechanism is detected according to testing agency so that mixed liquor from nozzle 6 spray form droplet-like after,
As desired to certain droplet into line displacement, so as to obtain required drop.The sorting mechanism can be inclined using electric field
Turn the sorting mechanism of principle, or use the sorting mechanism of ultrasound, vibrations or air pressure principle.
In one embodiment of the invention, sorting mechanism includes the charging electrode 7 and deflecting electrode from top to bottom set, deflection
Electrode includes, deflecting electrode cathode 8 and deflecting electrode anode 9.Charging electrode 7 is used to charge to drop, and deflecting electrode is used
In to electrically charged drop into horizontal deflection.
Further, charging electrode 5 is loop configuration, so as to the deflection of drop.
Motion is triaxial movement platform, and triaxial movement platform is connected with accumulation platform 8, with 8 edge of driving accumulation platform
Three-axis moving；Or triaxial movement platform is connected with nozzle 1, to drive nozzle along three-axis moving.
Further, the biological 3D printing system further includes the retracting device 11 for being arranged at the lower section of deflecting electrode 6, recycling
Device 11 is used to recycle undesirable drop after sorting mechanism sorts.
Retracting device 7 includes accumulator tank and recovery pump, and the recovery pump is used to recycle the mixed liquor in accumulator tank.
Particle diameter is generally 0.1 μm -1000 μm.The particle includes but not limited to cell, cell mass, boiomacromolecule
Group, cell embedding group, cell mass embedding group, boiomacromolecule embedding group.
The present invention operation principle be：
Particle is mixed in liquid, is sent into by sample inlet 2 in runner, then sample liquid is sprayed by nozzle 6.When
When grain flows through Kurt testing agency 4, the impedance between first electrode 12 and second electrode 14 can be changed, by detecting interface 5
The change of the impedance can be measured, is with information, controls such as sizes at the time of obtaining particle by the impedance variations information
System carries out ensuing sorting according to these information.
The piezoelectric ceramics that nozzle 1 is installed forms disturbance as perturbation mechanism at liquid, when the liquid is formed after ejection
Between and period profile spatially, at the drop separation moment, control system can load certain electricity on annular shaped charge electrode 7
Pressure, the liquid at this moment sprayed can induce a certain amount of electric charge, droplet formation and after separating, and Portable belt is a certain amount of on drop
Electric charge.When drop with electric charge is via deflecting electrode, due to the effect of electric field force, deviation can occur for drop route, a part
Drop is recycled by retracting device 11, and another part is fallen on accumulation platform 10.Retracting device 11 possesses a liquid
Groove, drop are recycled after injecting liquid tank by vacuum pump or peristaltic pump.Specific shape can be formed by the movement drop of motion
The accumulation of shape.
In conclusion the liquid for being mixed with cell is ejected via cavity, Perturbed systems disturb to form space, time
The drop of upper stabilization.Detecting system allows measurement by the cell or position and the speed of other particles in detection zone.Sorting
System can be directed to specific drop, be allowed to deviate original track by specific demand.Kinematic system allows the knot of cell accumulation
Structure is moved.The present invention can be achieved to be precisely controlled cell or other granular materials, and it is big, it can be achieved that high to print flux
Density prints.
The foregoing is merely embodiments of the present invention, are not intended to limit the scope of the present invention.It is all in the present invention
Spirit and principle within any modification, equivalent replacement, improvement, extension etc., be all contained in protection scope of the present invention
It is interior.
Claims (9)
- A kind of 1. biological 3D printing system based on Coulter principle, it is characterised in that including nozzle (1), perturbation mechanism (3), Testing agency (4), sorting mechanism, accumulation platform (10) and motion, wherein,The nozzle (1) is used to spray droplet-like or cylindrical particle mixed liquor；The perturbation mechanism (3) is arranged on the nozzle (1)；The testing agency (4) is arranged at the end of the nozzle (1), and the testing agency (4) is used to detect the nozzle (1) The particle of the particle mixed liquor of ejection；The sorting mechanism is arranged at the lower section of the testing agency (4), and the sorting mechanism is used for according to the testing agency (4) particle information of detection, by satisfactory drop into line displacement, so as to obtain required drop；The accumulation platform (10) is arranged at the lower section of the sorting mechanism, is sub-elected by the sorting mechanism satisfactory Drop is piled into required shape on the accumulation platform (10)；The motion can make nozzle (1) carry out relative motion with the accumulation platform (10), so that particle can be according to Space carries out the accumulation of setting shape.
- 2. the biological 3D printing system according to claim 1 based on Coulter principle, it is characterised in that the detection machine Structure (4) includes first electrode (12), second electrode (14) and is arranged between the first electrode (12) and second electrode (14) Insulator (13), the first electrode (12) is an integral structure with the nozzle (1), the first electrode (12) and second Electrode (14) draws detection interface (5) by conducting wire.
- 3. the biological 3D printing system according to claim 1 based on Coulter principle, it is characterised in that the nozzle (1) runner is equipped with, the upper end of the runner is connected with sample inlet (2), and lower end is connected with nozzle (6).
- 4. the biological 3D printing system according to claim 1 based on Coulter principle, it is characterised in that the disturbance machine Structure (3) is piezoelectric ceramics, and the piezoelectric ceramics is embedded in the annular groove that the nozzle (1) outer surface is equipped with.
- 5. the biological 3D printing system according to claim 1 based on Coulter principle, it is characterised in that the sorting machine Structure includes the charging electrode (7) and deflecting electrode from top to bottom set, and the charging electrode (7) is used to charge to drop, The deflecting electrode is used for electrically charged drop into horizontal deflection.
- 6. the biological 3D printing system according to claim 5 based on Coulter principle, it is characterised in that the charging electricity Pole (7) is loop configuration.
- 7. the biological 3D printing system according to claim 1 based on Coulter principle, it is characterised in that the fitness machine Structure is triaxial movement platform, and the triaxial movement platform is connected with the accumulation platform (10) or the nozzle (1).
- 8. the biological 3D printing system according to claim 1 based on Coulter principle, it is characterised in that further include setting Retracting device (11) below the deflecting electrode, the retracting device (11) are used to recycle after the sorting mechanism sorts not Satisfactory drop.
- 9. the biological 3D printing system according to claim 8 based on Coulter principle, it is characterised in that the recycling dress Putting (7) includes accumulator tank and recovery pump, and the recovery pump is used to recycle the mixed liquor in the accumulator tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711369736.6A CN107962770A (en) | 2017-12-18 | 2017-12-18 | A kind of biological 3D printing system based on Coulter principle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711369736.6A CN107962770A (en) | 2017-12-18 | 2017-12-18 | A kind of biological 3D printing system based on Coulter principle |
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| CN107962770A true CN107962770A (en) | 2018-04-27 |
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| CN201711369736.6A Pending CN107962770A (en) | 2017-12-18 | 2017-12-18 | A kind of biological 3D printing system based on Coulter principle |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109049674A (en) * | 2018-10-18 | 2018-12-21 | 吉林大学 | A kind of increasing material manufacturing device and method for micro-system three-dimensional structure |
| CN109228305A (en) * | 2018-09-28 | 2019-01-18 | 大连理工大学 | A kind of 3 D-printing method of electric field induction auxiliary electrojet |
| CN109228304A (en) * | 2018-09-28 | 2019-01-18 | 大连理工大学 | A kind of 3 D-printing device of electric field induction auxiliary electrojet |
| CN112916288A (en) * | 2021-01-19 | 2021-06-08 | 华中科技大学 | Piezoelectric type monodisperse continuous droplet generator |
| EP3868544A1 (en) * | 2020-02-19 | 2021-08-25 | Ricoh Company, Ltd. | Three-dimensional modeling apparatus and three-dimensional modeling method |
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| CN107245430A (en) * | 2017-06-23 | 2017-10-13 | 广州迈普再生医学科技有限公司 | A kind of cell 3D printing system and Method of printing |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107245430A (en) * | 2017-06-23 | 2017-10-13 | 广州迈普再生医学科技有限公司 | A kind of cell 3D printing system and Method of printing |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109228305A (en) * | 2018-09-28 | 2019-01-18 | 大连理工大学 | A kind of 3 D-printing method of electric field induction auxiliary electrojet |
| CN109228304A (en) * | 2018-09-28 | 2019-01-18 | 大连理工大学 | A kind of 3 D-printing device of electric field induction auxiliary electrojet |
| CN109228305B (en) * | 2018-09-28 | 2020-04-28 | 大连理工大学 | Three-dimensional printing method for electric field induced auxiliary electrospray |
| CN109049674A (en) * | 2018-10-18 | 2018-12-21 | 吉林大学 | A kind of increasing material manufacturing device and method for micro-system three-dimensional structure |
| CN109049674B (en) * | 2018-10-18 | 2023-08-01 | 吉林大学 | Additive manufacturing device and method for microsystem three-dimensional structure |
| EP3868544A1 (en) * | 2020-02-19 | 2021-08-25 | Ricoh Company, Ltd. | Three-dimensional modeling apparatus and three-dimensional modeling method |
| US11958242B2 (en) | 2020-02-19 | 2024-04-16 | Ricoh Company, Ltd. | Three-dimensional modeling apparatus and three-dimensional modeling method |
| CN112916288A (en) * | 2021-01-19 | 2021-06-08 | 华中科技大学 | Piezoelectric type monodisperse continuous droplet generator |
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Application publication date: 20180427 |
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