CN107960527A - It is a kind of to be spray-dried the method for preparing Bradley yeast microcapsules - Google Patents
It is a kind of to be spray-dried the method for preparing Bradley yeast microcapsules Download PDFInfo
- Publication number
- CN107960527A CN107960527A CN201711216278.2A CN201711216278A CN107960527A CN 107960527 A CN107960527 A CN 107960527A CN 201711216278 A CN201711216278 A CN 201711216278A CN 107960527 A CN107960527 A CN 107960527A
- Authority
- CN
- China
- Prior art keywords
- bradley yeast
- bradley
- wall material
- spray drying
- microcapsules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
Abstract
The invention discloses a kind of method for being spray-dried and preparing Bradley yeast microcapsules, the Bradley yeast microcapsules are using Bradley yeast as core, using modified tapioca starch, beta cyclodextrin, gelatin as wall material, polysorbate60 is emulsifying agent, is prepared using drying process with atomizing technology.The preparation method technique is simple, of low cost, easily controllable and storage, stability are strong, safe and reliable, easily controllable and large-scale production, relative to traditional desivac, extrusion, this method equipment requirement and of low cost, raw material easily obtains, it is simple to operate, it is more advantageous to reducing the loss of saccharomyces boulardii in production process；The Bradley yeast microcapsules of gained are prepared at the same time, uniform particle sizes, with stronger acid resistance and stability, bacterium activity is high, survival period is grown, the effect of saccharomyces boulardii in vivo is substantially increased, can be widely used for the fields such as biological medicine, food processing, especially there is potential application in terms of domestic fowl farming feed addictive.
Description
【Technical field】
The invention belongs to microbial technology field, and in particular to a kind of to be spray-dried the method for preparing Bradley yeast microcapsules.
【Background technology】
Saccharomyces boulardii belongs to a kind of unique subspecies in saccharomyces cerevisiae, and fast with reproduction speed, vitality is strong, 24h, that is, reachable
The advantages that to the stage of stable development.It contains the nutriments such as abundant protein, amino acid, vitamin and various digestive ferments, frequently as
Human body probiotics is widely applied to biomedicine field, especially treats acute infectious diarrhea, irritable bowel syndrome, prevention
With using the relevant colitis of broad spectrum antibiotic and diarrhea etc..It is well known that saccharomyces boulardii must lead in use
Cross gastric environment and reach the effect of enteron aisle competence exertion, and hydrochloric acid in gastric juice has bactericidal effect in human body, this results in it when reaching enteron aisle
Viable bacteria number is greatly reduced, and this not only lowers the medicinal properties of saccharomyces boulardii, and greatly limit its application space.
Therefore, how to ensure that Bradley yeast viable bacteria can resist hydrochloric acid in gastric juice and smoothly reach enteron aisle and play a role becomes the weight of people's research
Point.
In recent years, probiotic microcapsule is turned to the pass that a kind of maximally efficient and development potentiality method is increasingly subject to people
Note, it can effectively obstruct the influence of external environment, greatly improve probiotics survival rate, acid resistance and enteric characteristics.It is but vertical
The microcapsule method for seeing current widespread reports mainly has desivac and extrusion, this generally requires special equipment and of high cost
High, low production efficiency and the probiotic granulate prepared are uneven, this had both been unfavorable for industrial operation and large-scale production, also pole
Its application in the field such as biological medicine and food processing is limited greatly.Therefore, a kind of technique of exploitation design is simple, cost is low
The strong Bradley yeast microcapsule preparation method of honest and clean, easily controllable and storage, stability is of great practical significance.
【The content of the invention】
The problem to be solved in the present invention be for more than deficiency, there is provided one kind using Bradley yeast as core, with modified tapioca starch,
Beta-cyclodextrin, gelatin are wall material, and polysorbate60 is emulsifying agent, technique is simple, of low cost, easily controllable and storage, stability are strong,
The method that safe and reliable spray drying prepares Bradley yeast microcapsules.
The technical solution adopted by the present invention is as follows：A kind of method for being spray-dried preparation Bradley yeast microcapsules, including with
Lower step：
（1）Bradley yeast activation, propagation and fermentation liquor treatment
The saccharomyces boulardii strain activated is linked into fermentation medium with 2% inoculum concentration, using cellar culture mode culture
24h obtains high density fermentation liquid to exponential phase；Zymotic fluid is put into 4 DEG C of centrifuges and is centrifuged, is utilized after the completion of centrifugation
PBS buffer washs to obtain Bradley yeast thalline core, and it is spare that gained Bradley yeast thalline core then is placed in 4 DEG C of refrigerators；
（2）The preparation of microcapsule wall material
Using modified tapioca starch, beta-cyclodextrin, gelatin as wall material, dissolved in deionized water, at room temperature for 14 ~ 18% by concentration
The wall material solution that stabilization is made is mixed evenly, obtained wall material solution is then put into sterilization treatment in 115 DEG C of autoclaves；
（3）Prepare plastc ring
Bradley yeast thalline core obtained above is added in microcapsule wall material solution, is mixed evenly, adds tween
60 are used as emulsifying agent to obtain emulsion, and emulsion is put into progress emulsifying in homogenizer obtains bacteria suspension；
（4）Spray drying
Bacteria suspension obtained above is subjected to spray drying treatment under 200 kpa atomizing pressures Bradley yeast microcapsules are made；
（5）Bradley yeast microcapsules obtained by above-mentioned spray drying are collected, scattered packaging prepares microcapsule feed additive.
Specifically, the step（1）Middle centrifuge speed is 4500-5000r/min, centrifugation time 5-8min, PBS
Buffer solution washing times are 2-3 times.
Specifically, the step（2）Middle modified tapioca starch, beta-cyclodextrin, gelatin mass ratio are 6:3:5, mixing speed
For 3000-3500r/min, mixing time 5-6min, sterilization time 15-20min.
Specifically, the step（3）The middle mass fraction for adding saccharomyces boulardii is 30-35%, mixing speed 3000-
3500r/min, the addition of emulsifying agent account for the 0.5-0.8% of wall material solution quality, and the homogenization speed of homogenizer is 8000-
10000r/min。
Specifically, the step（4）75-80 DEG C of intake air temperature during middle spray drying treatment, air outlet temperature 65-70
DEG C, feed rate 15-18mL/min.
It is an advantage of the invention that：
1. the present invention using saccharomyces boulardii as core, using modified tapioca starch, beta-cyclodextrin, gelatin as wall material, using polysorbate60 as
Emulsifying agent, using conventional drying process with atomizing and microcapsules technology during preparation, the preparation method technique is simple, it is of low cost,
Stability is strong, safe and reliable, easily controllable and large-scale production.
2. it is wall material that the present invention, which selects modified tapioca starch, beta-cyclodextrin, gelatin, of low cost, it is easily obtained, safety is strong
Health, it is applied widely, it can be widely used for the fields such as biological medicine, food processing.
3. the Bradley yeast microcapsules that the present invention is prepared using spray drying technology, relative to traditional desivac, extruding
Method, equipment requirement and cost are cheaper, simple to operate, advantageously reduce the loss of saccharomyces boulardii in production process.
4. the present invention prepares the Bradley yeast microcapsules of gained, uniform particle sizes, have stronger acid resistance and stability,
Bacterium activity is high, and survival period length, more helps to improve the effect of saccharomyces boulardii in vivo, while in domestic fowl farming feed addictive
Aspect has potential application.
【Brief description of the drawings】
Fig. 1 is the process flow chart of the present invention.
【Embodiment】
In order to more fully understand the present invention technology contents, below by specific embodiment to technical solution of the present invention into advance one
Step is introduced and explanation.Following embodiments are descriptive, are not limited, it is impossible to the protection model of the present invention is limited with this
Enclose.
Embodiment 1
（1）Bradley yeast activation, propagation and fermentation liquor treatment
The saccharomyces boulardii strain activated is linked into fermentation tank with 2% inoculum concentration, using cellar culture mode culture 24h
To exponential phase, high density fermentation liquid is obtained；By zymotic fluid be put into 4 DEG C of centrifuges under 4500r/min centrifugal speeds from
Heart 5min, is washed 2 times using PBS buffer after the completion of centrifugation and obtains Bradley yeast thalline core, then by gained Bradley yeast
It is spare that thalline core is placed in 4 DEG C of refrigerators；
（2）Mix the preparation of wall material
Using mass ratio as 6:3:5 modified tapioca starch, beta-cyclodextrin, gelatin are dissolved in as wall material by concentration for 14%
In ionized water, 5min is mixed with 3000r/min mixing speeds at ambient temperature, it is molten to stir evenly the wall material for being made stable
Liquid, is then put into 115 DEG C of autoclaves the 15min that sterilizes by obtained wall material solution；
（3）Prepare plastc ring
By step（1）Obtained in Bradley yeast thalline core be added to step according to the ratio of mass fraction 30%（2）It is made
Microcapsule wall material solution in, be mixed evenly under 3000r/min mixing speeds, then add account for wall material solution quality
Emulsion is made as emulsifying agent in 0.5% polysorbate60, emulsion is put into emulsifying in 8000r/min homogenizers obtains bacterium and hang
Liquid；
（4）Spray drying
By step（3）In the bacteria suspension for preparing carry out spray drying treatment, 75 DEG C of intake air temperature, 65 DEG C of air outlet temperature,
Feed rate is 15mL/min, nebulizer pressure 200kpa.
（5）Bradley yeast microcapsules obtained by above-mentioned spray drying are collected, scattered packaging prepares microcapsule feed additive.
（6）Detect Bradley yeast microcapsules viable count and Bradley yeast microcapsules obtained above respectively in simulate the gastric juice and
Viable count is shown in Table 1 after simulated intestinal fluid processing.
Embodiment two
（1）Bradley yeast activation, propagation and fermentation liquor treatment
The saccharomyces boulardii strain activated is linked into fermentation tank with 2% inoculum concentration, using cellar culture mode culture 24h
To exponential phase, high density fermentation liquid is obtained；By zymotic fluid be put into 4 DEG C of centrifuges under 4800r/min centrifugal speeds from
Heart 6min, is washed 2 times using PBS buffer after the completion of centrifugation and obtains Bradley yeast thalline core, then by gained Bradley yeast
It is spare that thalline core is placed in 4 DEG C of refrigerators；
（2）Mix the preparation of wall material
Using mass ratio as 6:3:5 modified tapioca starch, beta-cyclodextrin, gelatin are dissolved in as wall material by concentration for 15%
In ionized water, 6min is mixed with 3200r/min mixing speeds at ambient temperature, it is molten to stir evenly the wall material for being made stable
Liquid, is then put into 115 DEG C of autoclaves the 18min that sterilizes by obtained wall material solution；
（3）Prepare plastc ring
By step（1）Obtained in Bradley yeast thalline core be added to step according to the ratio of mass fraction 32%（2）It is made
Microcapsule wall material solution in, be mixed evenly under 3200r/min mixing speeds, then add account for wall material solution quality
Emulsion is made as emulsifying agent in 0.6% polysorbate60, emulsion is put into emulsifying in 9000r/min homogenizers obtains bacterium and hang
Liquid；
（4）Spray drying
By step（3）In the bacteria suspension for preparing carry out spray drying treatment, 77 DEG C of intake air temperature, 67 DEG C of air outlet temperature,
Feed rate is 16mL/min, nebulizer pressure 200kpa.
（5）Bradley yeast microcapsules obtained by above-mentioned spray drying are collected, scattered packaging prepares microcapsule feed additive.
（6）Detect Bradley yeast microcapsules viable count and Bradley yeast microcapsules obtained above respectively in simulate the gastric juice and
Viable count is shown in Table 1 after simulated intestinal fluid processing.
Embodiment three
（1）Bradley yeast activation, propagation and fermentation liquor treatment
The saccharomyces boulardii strain activated is linked into fermentation tank with 2% inoculum concentration, using cellar culture mode culture 24h
To exponential phase, high density fermentation liquid is obtained；By zymotic fluid be put into 4 DEG C of centrifuges under 5000r/min centrifugal speeds from
Heart 8min, is washed 3 times using PBS buffer after the completion of centrifugation and obtains Bradley yeast thalline core, then by gained Bradley yeast
It is spare that thalline core is placed in 4 DEG C of refrigerators；
（2）Mix the preparation of wall material
Using mass ratio as 6:3:5 modified tapioca starch, beta-cyclodextrin, gelatin are dissolved in as wall material by concentration for 18%
In ionized water, 6min is mixed with 3500r/min mixing speeds at ambient temperature, it is molten to stir evenly the wall material for being made stable
Liquid, is then put into 115 DEG C of autoclaves the 20min that sterilizes by obtained wall material solution；
（3）Prepare plastc ring
By step（1）Obtained in Bradley yeast thalline core be added to step according to the ratio of mass fraction 35%（2）It is made
Microcapsule wall material solution in, be mixed evenly under 3500r/min mixing speeds, then add account for wall material solution quality
Emulsion is made as emulsifying agent in 0.8% polysorbate60, emulsion is put into emulsifying in 10000r/min homogenizers obtains bacterium and hang
Liquid；
（4）Spray drying
By step（3）In the bacteria suspension for preparing carry out spray drying treatment, 80 DEG C of intake air temperature, 70 DEG C of air outlet temperature,
Feed rate is 18mL/min, nebulizer pressure 200kpa.
（5）Bradley yeast microcapsules obtained by above-mentioned spray drying are collected, scattered packaging prepares microcapsule feed additive.
（6）Detect Bradley yeast microcapsules viable count and Bradley yeast microcapsules obtained above respectively in simulate the gastric juice and
Viable count is shown in Table 1 after simulated intestinal fluid processing.
Table 1 for the Bradley yeast microcapsules viable count for preparing of the present invention and Bradley yeast microcapsules respectively in simulate the gastric juice and
Viable count after simulated intestinal fluid processing.
Viable count mesh assay method is as follows：1g Bradley yeast microcapsule formulations are taken to be dissolved in 100 mL sterile PBS buffers,
Fully vibration mixing, 10 times of gradient dilution, then take 0.1mL dilutions spread plate to be trained to YPD culture mediums, all tablets at 30 DEG C
48h is supported with to be counted.
As shown in Table 1, the Bradley yeast microcapsule bacterial activity that prepared by the present invention is high, while it is respectively in simulate the gastric juice and mould
Still there is higher viable count after intending intestinal juice processing.
Embodiment described above only expresses embodiments of the present invention, its description is more specific and detailed, but can not
Therefore it is interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that for those of ordinary skill in the art,
On the premise of this design is not departed from, some improvement can also be made, these belong to protection scope of the present invention.Therefore, originally
The protection domain of patent of invention should be determined by the appended claims.
Claims (6)
1. a kind of be spray-dried the method for preparing Bradley yeast microcapsules, it is characterised in that comprises the following steps：
（1）Bradley yeast activation, propagation and fermentation liquor treatment
The saccharomyces boulardii strain activated is linked into fermentation medium with 2% inoculum concentration, using cellar culture mode culture
24h obtains high density fermentation liquid to exponential phase；Zymotic fluid is put into 4 DEG C of centrifuges and is centrifuged, is utilized after the completion of centrifugation
PBS buffer washs to obtain Bradley yeast thalline core, and it is spare that gained Bradley yeast thalline core then is placed in 4 DEG C of refrigerators；
（2）The preparation of microcapsule wall material
Using modified tapioca starch, beta-cyclodextrin, gelatin as wall material, dissolved in deionized water, at room temperature for 14 ~ 18% by concentration
Obtained wall material solution is mixed evenly, obtained wall material solution is then put into sterilization treatment in 115 DEG C of autoclaves；
（3）Prepare plastc ring
Bradley yeast thalline core obtained above is added in microcapsule wall material solution, is mixed evenly, adds tween
60 are used as emulsifying agent to obtain emulsion, and emulsion is put into progress emulsifying in homogenizer obtains bacteria suspension；
（4）Spray drying
Bacteria suspension obtained above is subjected to spray drying treatment under 200 kpa atomizing pressures Bradley yeast microcapsules are made；
（5）Bradley yeast microcapsules obtained by above-mentioned spray drying are collected, scattered packaging prepares microcapsule feed additive.
2. the method that spray drying as claimed in claim 1 prepares Bradley yeast microcapsules, it is characterised in that：The step
（1）Middle centrifuge speed is 4500-5000r/min, and centrifugation time 5-8min, PBS buffer washing times are 2-3 times.
3. the method that spray drying as claimed in claim 1 prepares Bradley yeast microcapsules, it is characterised in that：The step
（2）Middle modified tapioca starch, beta-cyclodextrin, gelatin mass ratio are 6:3:5, mixing speed 3000-3500r/min, stirring
Time is 5-6min, sterilization time 15-20min.
4. the method that spray drying as claimed in claim 1 prepares Bradley yeast microcapsules, it is characterised in that：The step
（3）The middle mass fraction for adding saccharomyces boulardii is 30-35%, mixing speed 3000-3500r/min, the addition of emulsifying agent
The 0.5-0.8% of wall material solution quality is accounted for, the homogenization speed of homogenizer is 8000-10000r/min.
5. the method that spray drying as claimed in claim 1 prepares Bradley yeast microcapsules, it is characterised in that：The step
（4）75-80 DEG C of intake air temperature during middle spray drying treatment, 65-70 DEG C of air outlet temperature, feed rate 15-18mL/min.
6. such as claim 1-5 any one of them Bradley yeast microcapsules answering in livestock and poultry cultivation as feed addictive
With.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711216278.2A CN107960527A (en) | 2017-11-28 | 2017-11-28 | It is a kind of to be spray-dried the method for preparing Bradley yeast microcapsules |
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| CN201711216278.2A CN107960527A (en) | 2017-11-28 | 2017-11-28 | It is a kind of to be spray-dried the method for preparing Bradley yeast microcapsules |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112603A (en) * | 1993-07-12 | 1995-11-29 | 圭马斯食品股份有限公司 | Food ingredients obtained by fermentation with S. boulardii and foods containing them |
| CN101766670A (en) * | 2010-02-23 | 2010-07-07 | 上海应用技术学院 | Rhodiola rosea polyphenol microcapsule and preparation method |
| US20110129518A1 (en) * | 2004-07-08 | 2011-06-02 | Imagilin Technology Llc | Probiotic products for pet applications |
| CN102550826A (en) * | 2012-02-07 | 2012-07-11 | 山东盛泰生物科技有限公司 | Active dry saccharomyces boulardii feed additive and its preparation method and use |
| CN104263716A (en) * | 2014-09-26 | 2015-01-07 | 天津生机集团股份有限公司 | Method for preparing butyric acid bacteria microcapsule bacteria powder |
| WO2017093148A1 (en) * | 2015-12-01 | 2017-06-08 | Ingwermat Limited | Molluscicidal composition containing encapsulated aliphatic aldehyde |
| US20170296598A1 (en) * | 2016-04-19 | 2017-10-19 | Akay Flavours and Aromatics Pvt, Ltd | Method of preparing stable, water soluble probiotic compositions based on millets and similar cereals |
-
2017
- 2017-11-28 CN CN201711216278.2A patent/CN107960527A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112603A (en) * | 1993-07-12 | 1995-11-29 | 圭马斯食品股份有限公司 | Food ingredients obtained by fermentation with S. boulardii and foods containing them |
| US20110129518A1 (en) * | 2004-07-08 | 2011-06-02 | Imagilin Technology Llc | Probiotic products for pet applications |
| CN101766670A (en) * | 2010-02-23 | 2010-07-07 | 上海应用技术学院 | Rhodiola rosea polyphenol microcapsule and preparation method |
| CN102550826A (en) * | 2012-02-07 | 2012-07-11 | 山东盛泰生物科技有限公司 | Active dry saccharomyces boulardii feed additive and its preparation method and use |
| CN104263716A (en) * | 2014-09-26 | 2015-01-07 | 天津生机集团股份有限公司 | Method for preparing butyric acid bacteria microcapsule bacteria powder |
| WO2017093148A1 (en) * | 2015-12-01 | 2017-06-08 | Ingwermat Limited | Molluscicidal composition containing encapsulated aliphatic aldehyde |
| US20170296598A1 (en) * | 2016-04-19 | 2017-10-19 | Akay Flavours and Aromatics Pvt, Ltd | Method of preparing stable, water soluble probiotic compositions based on millets and similar cereals |
Non-Patent Citations (1)
| Title |
|---|
| SULTAN ARSLAN ET AL: "Microencapsulation of probiotic Saccharomyces cerevisiae var. boulardii with different wall materials by spray drying", 《FOOD SCIENCE AND TECHNOLOGY》 * |
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