CN107960455A - A kind of new lotus rhizome prevent-browning preservation method - Google Patents
A kind of new lotus rhizome prevent-browning preservation method Download PDFInfo
- Publication number
- CN107960455A CN107960455A CN201710858863.6A CN201710858863A CN107960455A CN 107960455 A CN107960455 A CN 107960455A CN 201710858863 A CN201710858863 A CN 201710858863A CN 107960455 A CN107960455 A CN 107960455A
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- lotus rhizome
- parts
- browning
- plasma
- prevent
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/015—Preserving by irradiation or electric treatment without heating effect
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B9/00—Preservation of edible seeds, e.g. cereals
- A23B9/16—Preserving with chemicals
- A23B9/24—Preserving with chemicals in the form of liquids or solids
- A23B9/30—Inorganic compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D1/00—Coating compositions, e.g. paints, varnishes or lacquers, based on inorganic substances
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/08—Anti-corrosive paints
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention discloses a kind of new lotus rhizome prevent-browning preservation method, including：The preparation of plasma, handle, prepare antistaling agent, post processing.Have the beneficial effect that：The active material of low-temperature plasma weight can react with group with the amino acid group of the polyphenol oxidase on lotus rhizome surface, so as to cause the forfeiture of enzymatic activity, hinder polyphenol oxidase to prevent the brown stain of lotus rhizome to the oxidation deterioration of aldehydes matter；It can make microbial cell plasma membrane and phosphatide cpd biosynthesis block with useful effect in microbial cell plasma membrane, cause cell membrane content to leak, seriously cause cell cracking, kill microorganism, play the role of preservation and antisepsis in antistaling agent；The method of the present invention is easy to operate; scale operation is can be applied to, prevent-browning fresh-keeping effect is notable, and antistaling agent is easily leached elution for water-soluble substances; absorbed even if can also be degraded in into human body by human internal environment, be a kind of new lotus rhizome prevent-browning preservation method efficiently, safe.
Description
Technical field
The present invention relates to field of food preservation, more particularly, to a kind of new lotus rhizome prevent-browning preservation method.
Technical background
Lotus rhizome originates in India, is just passed to China very early.Lotus rhizome belongs to Magnoliidae, the wild mesh of mountain dragon.Happiness is warm, not shade tolerant, no
Suitable water shortage, strong wind, lotus rhizome is slightly sweet and crisp, can eat raw and also cook, and medical value is at a relatively high, its Gen Genyeye, stamen or pistil
Fruit, fixed-point-free power automorphism is precious, can all nourish and be used as medicine.Powder is made with lotus rhizome, can help digestion antidiarrheal, and heat-clearing of whetting the appetite, nourishing is nourished one's nature, in prevention
Bleeding, is the first-class supping of women and children child old woman, valetudinarian and the good treasure of nourishing.The nutritional ingredient of lotus rhizome is extremely abundant, often
It is 77.9 grams of moisture content in hectogram lotus rhizome, 1.0 grams of protein, 0.1 gram of fat, 19.8 grams of carbohydrate, 84 kilocalories of heat, thick
0.5 gram of fiber, 0.7 gram of ash content, 19 milligrams of calcium, 51 milligrams of phosphorus, 0.5 milligram of iron, 0.02 milligram of carrotene, thiamine 0.11
Milligram, 0.04 milligram of riboflavin, 0.4 milligram of niacin, 25 milligrams of ascorbic acid.Lotus rhizome also has very high medical value, raw
Lotus root is cold in nature sweet in flavor, can dissolving stasis, heat-clearing, promote the production of body fluid, preventing or arresting vomiting, cough-relieving；Ripe lotus root is warm-natured, there is spleen benefiting and stimulating the appetite, beneficial blood myogenic, hemostasis stasis
The effect of, therefore, lotus rhizome is not only quality vegetables, but also have the characteristic of superior fruit concurrently, and union nutrition and medicine are used for one, can be rated as
Fruit and vegetable, medicine three are all good, are a kind of food of food and medicine consangunity.
The enzymatic browning as caused by polyphenol oxidase is to cause the main original of lotus rhizome quality decline during lotus rhizome postharvest storage
Cause.It is generally believed that enzymatic browning refers to that polyphenol oxidase (PPO) in the presence of oxygen, phenolic compound is oxidized to face benzene
Quinone and cutting surface slight brown stain is occurred, then led again by complicated quinone polymerisation to form substantial amounts of brown material
Cause brown stain.The technology of lotus rhizome prevent-browning mainly has physical method such as low temperature, ozone, controlled atmospheric packing, vacuum packaging, control at present
Humidity, ultrasonic wave, ultraviolet etc., chemical method is such as：Chemical preservative, crude antistaling agent etc., biological method such as zymotechnic, base
The methods of because of engineering technology, but existing method generally existing is of high cost, fresh-keeping effect is undesirable, Consumer acceptance is poor etc. asks
Topic.Therefore, it is necessary to further develop new efficient, convenient, environmentally friendly new method.
Technology on lotus rhizome prevent-browning has many methods, and the prior art is as authorized public platform
The Chinese invention patent of 101341904 B of CN, discloses a kind of method for suppressing brown stain of cut lotus root, the inventive method
By fresh lotus rhizome is selected, cleaning, after cutting and disinfection, after temperature is 48-55 DEG C of hot water treatment 4-10 minute, cools down,
Surface moisture is removed, then packs, refrigerate or direct circulation sale, has the advantages that easy to operate, environmental protection is cheap, but its behaviour
Make that method is excessively simple and unadorned, simply with 48-55 DEG C of the 4-10 minutes polyphenol oxidases that can not inactivate lotus rhizome section completely of hot water treatment
Enzyme, oxidation aldehydes matter still can be oxidized to quinones substance by polyphenol oxidase, and then quinone polymerisation, which occurs, causes lotus rhizome to be cut
Face brown stain, therefore the actual application value of the inventive method is little.
The content of the invention
The purpose of this method is to provide a kind of new lotus rhizome prevent-browning using lower temperature plasma technology processing lotus rhizome
Preservation method.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：A kind of new lotus rhizome prevent-browning is protected
Fresh method, including：The preparation of plasma, handle, prepare antistaling agent, post processing, specifically includes following steps：
The preparation of plasma：Using dielectric barrier discharge configuration device as plasma source, its frequency is 2.45-
2.55GHz, power 1.2-1.25kW, its sparking mode are igniting/pause：4-5s/6-7s, time cycle 20-25
A cycle, the humidified device of plasma are stand-by after being humidified with water；In plasma containing substantial amounts of active oxygen, ultraviolet photon,
OH、O2 -, the high active substance such as HOO, NO free radical, prepare for further Preservation Treatment；
Processing：Not damaged, disease-free, without brown stain, without shrink fresh lotus rhizome are taken, and keeps rhizoma nelumbinis complete, with 2- after cleaning
40ppm sterilizes water sterilization, and lotus rhizome surface liquid is drained and is placed on metallic plate with holes, surface on a metal plate has one
Layer antioxidant anticorrosive coating, its component are：80-100 parts of distilled water, 50-60 parts of aluminium dihydrogen phosphate, ethyl orthosilicate 0.5-0.6
Part, 0.8-1.0 parts of magnesia, 0.6-0.7 parts of zinc oxide, 2.4-2.5 parts of magnesium chromate, 4.5-4.8 parts of micro-nano aluminum powder, 3- (4-
Anisyl) 0.05-0.06 parts of -3- ethyl 3-oxopropanoates, metallic plate and lotus rhizome are immediately placed in sealable processing at room temperature
In room, the pressure of process chamber is evacuated to by 1-10Pa with vacuum pump, is then injected into cooling plasma, after handling 7.5-10 minutes
Take out lotus rhizome；After plasma is contacted with lotus rhizome surface, active oxygen, ultraviolet photon, OH, O in plasma2 -、HOO、
Amino acid such as cysteine with active side chain in NO free yl induction polyphenol oxidase, the phenylalanine with aromatic rings,
Tyrosine, tryptophan react, and the forfeiture of enzymatic activity is caused with this, hinder oxidation of the polyphenol oxidase to aldehydes matter to become
Matter, so as to prevent the generation of lotus root browning；The antioxidant anticorrosive coating of metal sheet surface can hinder low temperature plasma active
Oxidation corrosion of the component to metal, extends the service life of metallic plate, and is also prevented from corrosion product to lotus rhizome component
It may influence；3- (4- anisyls) -3- ethyl 3-oxopropanoates can promote active ingredient and polyphenol oxidase in plasma
Enzyme reacts, and improves enzyme deactivation efficiency, the time of contact of plasma and lotus rhizome is reduced, so as to reduce cost.
Prepare antistaling agent：Weigh 0.15-0.2 parts of nisin, 0.3-0.5 parts of sodium phytate, sodium sorbate 0.3-
0.5 part, 3-6 parts of plant extracts, 30-50 parts of distilled water at a temperature of 30-35 DEG C uniformly mixing 10-15 minute to obtain the final product it is fresh-keeping
Agent；Sterilization component in antistaling agent can also useful effect in microbial cell plasma membrane, make microbial cell plasma membrane and phosphatide
Compound biosynthesis block, causes cell membrane content to leak, and seriously causes cell cracking, kills microorganism；Antistaling agent enters people
It can be degraded and absorbed after body, safe without toxic side effect；
Post processing：The lotus rhizome of plasma-treated mistake is immersed in antistaling agent, is 30-32kHz, power density in frequency
For 0.45-0.55W/cm2Ultrasound condition under soak 2-3 minutes after take out, lotus rhizome is immediately placed in 5-7 DEG C of preservation；Ultrasonic wave
" cavitation effect " accelerate microbial cell film film potential inside lotus rhizome and change and the increase of membrane permeability, effectively destroy
The membrane structure of microorganism so that the active ingredient in antistaling agent can be more rapidly introduced into inside microbial cell, destroyed
Eucaryotic cell structure, is killed, and effectively enhances bactericidal effect, can effectively extend the fresh-keeping time limit of lotus rhizome.
Preferably, disinfectant used is liquor natrii hypochloritis or sodium chloride solution or hydrogen peroxide solution or titanium dioxide
Solutions of chlorine sodium chloride solution weak acid electrolyte；Disinfectant can effectively kill harmful microorganism existing for lotus rhizome surface, and can
To be easily eluted removing.
Preferably, plant extracts used is flavone of hawthorn fruit or insults jujube flavones or Rosmarinus officinalis extract or konjaku flour
Or grape seed extract；Plant extracts contributes to preservation, and harmless.
Compared with prior art, the advantage of the invention is that：1) with rich in active oxygen, ultraviolet photon, OH, O2 -、HOO、NO
The Low Temperature Plasma Treating lotus rhizome of free radical isoreactivity material and group, active material can be with lotus rhizome surfaces with group
The amino acid group of polyphenol oxidase reacts, so as to cause the forfeiture of enzymatic activity, hinders polyphenol oxidase to aldehydes matter
Oxidation deterioration, prevent the brown stain of lotus rhizome；2) 3- (4- anisyls) in the antioxidant anticorrosive coating of metallic plate upper surface-
3- ethyl 3-oxopropanoates can promote the active ingredient in plasma to react with polyphenol oxidase, improve enzyme deactivation efficiency,
The time of contact of plasma and lotus rhizome is reduced, so as to reduce cost；3) ultrasonic wave can destroy the cell membrane of lotus rhizome microorganism
Structure, sterilization component in antistaling agent can also useful effect in microbial cell plasma membrane, make microbial cell plasma membrane and phosphatide
Compound synthesis is obstructed, and causes cell membrane content to leak, and seriously causes cell cracking, kills microorganism, plays fresh-keeping
Corrosion-resistant acts on；4) the method for the present invention is easy to operate, can be applied to scale operation, prevent-browning fresh-keeping effect is notable, fresh-keeping
Agent is easily leached elution for water-soluble substances, even if into can also be degraded to amino acid in human internal environment in human body
Absorption of human body is supplied etc. small molecule, safe without toxic side effect, therefore be a kind of new fresh-keeping side of lotus rhizome prevent-browning efficiently, safe
Method.
Brief description of the drawings
Fig. 1 be the polyphenol oxidase of lotus rhizome section of the present invention remaining enzymatic activity and 2- hydroxyls -3- tert-butyl benzenes formaldehyde with
The relation schematic diagram of the mass ratio of 3- (4- anisyls) -3- ethyl 3-oxopropanoates.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of new lotus rhizome prevent-browning preservation method, comprises the following steps：
1) low temperature plasma mixed gas is prepared；Contain substantial amounts of active oxygen, ultraviolet photon, OH, O in plasma2 -、
The high active substances such as HOO, NO free radical, prepare for further Preservation Treatment；2) take not damaged, it is disease-free, without brown stain,
Fresh lotus rhizome without shrink, and keep rhizoma nelumbinis complete, sterilized after cleaning with 2ppm hydrogen peroxide solutions, lotus rhizome surface liquid is dripped
It is dry to be placed on metallic plate with holes, metallic plate and lotus rhizome are immediately placed in sealable process chamber at room temperature, with vacuum pump
The pressure of process chamber is evacuated to 1Pa, is then injected into cooling plasma, lotus rhizome is taken out in processing after ten minutes；3) will be through plasma
The processed lotus rhizome of body is immersed in antistaling agent, and antistaling agent is 0.15 part of nisin, 0.3 part of sodium phytate, sodium sorbate
0.3 part, 3 parts of grape seed extract, the mixture of 30 parts of distilled water, frequency be 30kHz, power density 0.45W/cm2's
Taken out after being soaked 2 minutes under ultrasound condition, lotus rhizome is immediately placed in 5 DEG C of preservations；The fresh-keeping time limit of lotus rhizome can effectively be extended.
Embodiment 2：
A kind of new lotus rhizome prevent-browning preservation method, specifically includes following steps：
1) preparation of plasma：Using dielectric barrier discharge configuration device as plasma source, its frequency is 2.55GHz, electricity
Source power is 1.25kW, its sparking mode is igniting/pause：5s/7s, time cycle are 20 cycles, and plasma is humidified
Device is stand-by after being humidified with water；Contain substantial amounts of active oxygen, ultraviolet photon, OH, O in plasma2 -, HOO, NO free radical it is contour
Active material, prepares for further Preservation Treatment；
2) handle：Take not damaged, disease-free, without brown stain, without shrink fresh lotus rhizome, and keep rhizoma nelumbinis complete, after cleaning with
40ppm sodium chloride solutions sterilize, and lotus rhizome surface liquid is drained and is placed on metallic plate with holes, surface on a metal plate
There is one layer of antioxidant anticorrosive coating, its component is：Distilled water 80g, aluminium dihydrogen phosphate 50g, ethyl orthosilicate 0.5g, magnesia
0.8g, zinc oxide 0.6g, magnesium chromate 2.4g, micro-nano aluminum powder 4.5g, 3- (4- anisyls) -3- ethyl 3-oxopropanoates
Metallic plate and lotus rhizome, are immediately placed in sealable process chamber by 0.05g at room temperature, are taken out the pressure of process chamber with vacuum pump
To 10Pa, cooling plasma is then injected into, lotus rhizome is taken out in processing after ten minutes；After plasma is contacted with lotus rhizome surface,
Active oxygen, ultraviolet photon, OH, O in plasma2 -, in HOO, NO free yl induction polyphenol oxidase with active side chain
Amino acid such as cysteine, the phenylalanine with aromatic rings, tyrosine, tryptophan react, and cause enzymatic activity with this
Forfeiture, polyphenol oxidase is hindered to the oxidation deterioration of aldehydes matter, so as to prevent the generation of lotus root browning；3) prepare fresh-keeping
Agent：Nisin 0.2g, sodium phytate 0.3g, sodium sorbate 0.5g, konjaku flour 6g, distilled water 50g are weighed in 35 DEG C of temperature
It is lower uniformly to mix 15 minutes up to antistaling agent；Sterilization component in antistaling agent can also useful effect in microbial cell plasma membrane,
Make microbial cell plasma membrane and phosphatide cpd biosynthesis block, cause cell membrane content to leak, seriously cause cell to split
Solution, kills microorganism；Antistaling agent can be degraded and absorbed after entering human body, safe without toxic side effect；
4) post-process：The lotus rhizome of plasma-treated mistake is immersed in antistaling agent, frequency be 32kHz, power density be
0.55W/cm2Ultrasound condition under soak 3 minutes after take out, lotus rhizome is immediately placed in 5 DEG C of preservations；In antistaling agent it is effective into
Dividing to be rapidly introduced into inside microbial cell, destroy eucaryotic cell structure, killed, effectively enhance bactericidal effect, Ke Yiyou
Effect extends the fresh-keeping time limit of lotus rhizome.
Embodiment 3：
A kind of new lotus rhizome prevent-browning preservation method, including：The preparation of plasma, handle, prepare antistaling agent, post processing, tool
Body comprises the following steps：
The preparation of plasma：Using dielectric barrier discharge configuration device as plasma source, its frequency is 2.55GHz, power supply
Power is 1.25kW, its sparking mode is igniting/pause：4s/6s, time cycle are 25 cycles, the humidified device of plasma
It is stand-by after being humidified with water；Contain substantial amounts of active oxygen, ultraviolet photon, OH, O in plasma2 -, the contour work of HOO, NO free radical
Property material, prepare for further Preservation Treatment；
Processing：Take not damaged, disease-free, without brown stain, without shrink fresh lotus rhizome, and keep rhizoma nelumbinis complete, after cleaning with
20ppm ClO 2 solutions sterilize, and lotus rhizome surface liquid is drained and is placed on metallic plate with holes, table on a metal plate
There is one layer of antioxidant anticorrosive coating in face, its component is：80 parts of distilled water, 60 parts of aluminium dihydrogen phosphate, 0.6 part of ethyl orthosilicate, oxygen
Change 1.0 parts of magnesium, 0.7 part of zinc oxide, 2.5 parts of magnesium chromate, 4.8 parts of micro-nano aluminum powder, 3- (4- anisyls) -3- oxopropanoic acids
Metallic plate, is immediately placed in sealable by 0.06 part of ethyl ester, 0.12 part of 2- hydroxyl -3- tert-butyl benzenes formaldehyde with lotus rhizome at room temperature
In process chamber, the pressure of process chamber is evacuated to by 5Pa with vacuum pump, is then injected into cooling plasma, processing takes after 7.5 minutes
Go out lotus rhizome；After plasma is contacted with lotus rhizome surface, active oxygen, ultraviolet photon, OH, O in plasma2 -、HOO、 NO
Amino acid such as cysteine, the phenylalanine with aromatic rings, junket with active side chain in free yl induction polyphenol oxidase
Propylhomoserin, tryptophan react, and the forfeiture of enzymatic activity is caused with this, hinder oxidation deterioration of the polyphenol oxidase to aldehydes matter,
So as to prevent the generation of lotus root browning；The antioxidant anticorrosive coating of metal sheet surface can hinder low temperature plasma activity into
Oxidation corrosion point to metal, extends the service life of metallic plate, and be also prevented from corrosion product to lotus rhizome component can
It can influence；
Prepare antistaling agent：Weigh 0.15 part of nisin, 0.4 part of sodium phytate, 0.4 part of sodium sorbate, 5 parts of flavone of hawthorn fruit,
40 parts of distilled water uniformly mixes 15 minutes up to antistaling agent at a temperature of 30 DEG C；Sterilization component in antistaling agent can also be effective
Microbial cell plasma membrane is acted on, makes microbial cell plasma membrane and phosphatide cpd biosynthesis block, is caused outside cell membrane content
Let out, seriously cause cell cracking, kill microorganism；Antistaling agent can be degraded and absorbed after entering human body, safe and non-toxic secondary work
With；
Post processing：The lotus rhizome of plasma-treated mistake is immersed in antistaling agent, frequency be 32kHz, power density be
0.55W/cm2Ultrasound condition under soak 3 minutes after take out, lotus rhizome is immediately placed in 5 DEG C of preservations；" the cavitation effect of ultrasonic wave
Should " change of microbial cell film film potential and the increase of membrane permeability inside lotus rhizome are accelerated, effectively destroy the thin of microorganism
After birth structure so that the active ingredient in antistaling agent can be more rapidly introduced into inside microbial cell, destroy eucaryotic cell structure,
Killed, effectively enhance bactericidal effect, can effectively extend the fresh-keeping time limit of lotus rhizome.
Embodiment 4：
A kind of new lotus rhizome prevent-browning preservation method, including：Preparation, metal sheet surface processing, processing, the preparation of plasma
Antistaling agent, post processing, specifically include following steps：
1st, the preparation of plasma：Using dielectric barrier discharge configuration device as plasma source, its frequency is 2.50GHz, electricity
Source power is 1.25kW, its sparking mode is igniting/pause：5s/6s, time cycle are 20 cycles, and plasma is humidified
Device is stand-by after being humidified with water；Contain substantial amounts of active oxygen, ultraviolet photon, OH, O in plasma2 -, HOO, NO free radical it is contour
Active material, prepares for further Preservation Treatment；
2nd, metal sheet surface is handled：Weigh 10 parts of following components and be configured to coating solution respectively：100 parts of distilled water, biphosphate
60 parts of aluminium, 0.6 part of ethyl orthosilicate, 0.9 part of magnesia, 0.6 part of zinc oxide, 2.5 parts of magnesium chromate, 4.8 parts of micro-nano aluminum powder,
0.05 part of 3- (4- anisyls) -3- ethyl 3-oxopropanoates, be separately added into successively 0.05 part of 2- hydroxyl -3- tert-butyl benzenes formaldehyde,
0.10 part, 0.15 part ..., 0.40 part, 0.45 part, 0.50 part, be made containing 2- hydroxyls -3- tert-butyl benzenes formaldehyde and 3- (4-
Anisyl) -3- ethyl 3-oxopropanoates mass ratio be 1:1、2:1、3:1、……、9:1、10:1 mixture coating, will apply
Layer be coated in metal sheet surface after air-dry 48 it is small when, be subsequently placed in pH be 6.3 subacidity NaCl electrolysis liquid in 25
Second, it is put into togerther together with lotus rhizome rapidly pending in process chamber；
3rd, handle：Take not damaged, disease-free, without brown stain, without shrink fresh lotus rhizome, and keep rhizoma nelumbinis complete, after cleaning with
30ppm liquor natrii hypochloritises sterilize, and lotus rhizome surface liquid is drained and is placed on metallic plate with holes, at room temperature by metallic plate
It is immediately placed in lotus rhizome in sealable process chamber, the pressure of process chamber is evacuated to by 2Pa with vacuum pump, is then injected into cooling etc.
Gas ions, processing take out lotus rhizome after 7.5 minutes；After plasma is contacted with lotus rhizome surface, active oxygen in plasma,
Ultraviolet photon, OH, O2 -, amino acid such as cysteine, band with active side chain in HOO, NO free yl induction polyphenol oxidase
The phenylalanine, tyrosine, tryptophan for having aromatic rings react, and the forfeiture of enzymatic activity is caused with this, hinder polyphenol oxidase
To the oxidation deterioration of aldehydes matter, so as to prevent the generation of lotus root browning；The antioxidant anticorrosive coating of metal sheet surface can
Oxidation corrosion of the low temperature plasma active ingredient to metal is hindered, extends the service life of metallic plate, and be also prevented from
Corrosion product influences the possibility of lotus rhizome component；
4th, antistaling agent is prepared：Weigh 0.18 part of nisin, 0.4 part of sodium phytate, 0.4 part of sodium sorbate, plant extracts
4 parts, 35 parts of distilled water at a temperature of 32 DEG C uniformly mixing 10 minutes up to antistaling agent；Sterilization component in antistaling agent can also
Useful effect makes microbial cell plasma membrane and phosphatide cpd biosynthesis block, causes cell membrane content in microbial cell plasma membrane
Beyond the region of objective existence is let out, and seriously causes cell cracking, kills microorganism；Antistaling agent can be degraded and absorbed after entering human body, safe and non-toxic pair
Effect；
5th, post-process：The lotus rhizome of plasma-treated mistake is immersed in antistaling agent, frequency be 32kHz, power density be
0.55W/cm2Ultrasound condition under soak 3 minutes after take out, lotus rhizome is immediately placed in 5 DEG C of preservations；" the cavitation effect of ultrasonic wave
Should " change of microbial cell film film potential and the increase of membrane permeability inside lotus rhizome are accelerated, effectively destroy the thin of microorganism
After birth structure so that the active ingredient in antistaling agent can be more rapidly introduced into inside microbial cell, destroy eucaryotic cell structure,
Killed, effectively enhance bactericidal effect, can effectively extend the fresh-keeping time limit of lotus rhizome.
Take and be immediately placed in 5 DEG C of preservations after ten minutes as a control group with 50 DEG C of hot-water soaks after fresh-cut lotus root cleaning and sterilizing,
The elasticity modulus of crush test detection lotus rhizome section is utilized respectively to embodiment 1-4 and control group, utilizes spectrophotometry
The enzymatic activity of section polyphenol oxidase, by the fresh-keeping number of days of the lotus rhizome of embodiment 1-3 and control group arrange shown in table 1：
The fresh-keeping number of days of 1. embodiment 1-3 of table and control group
| Project | Control | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Non- brown stain number of days/day | 8 | 15 | 16 | 16 |
| 10% number of days of brown stain/day | 9 | 17 | 19 | 20 |
| 30% number of days of brown stain/day | 10 | 20 | 25 | 24 |
| 60% number of days of brown stain/day | 12 | 28 | 30 | 32 |
As shown in Table 1, compared to the hot-water soak of control group, the lotus root browning time after Low Temperature Plasma Treating is big
It is big to extend, and its brown stain speed substantially slows down, and illustrates that Low Temperature Plasma Treating has significantly attenuated lotus rhizome section polyphenol oxidase
The activity of enzyme, has delayed the brown stain process of lotus rhizome, has higher fresh-keeping meaning.
The remaining enzymatic activity of lotus rhizome section, the dry matter content of lotus rhizome, lotus rhizome in embodiment 1-3 after when measurement processing 24 is small
The elasticity modulus of block, arranges as shown in table 2：
Remaining enzymatic activity, dry matter content and the elasticity modulus of lotus rhizome in 2. embodiment 1-3 of table
| Project | Initial value | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Lotus rhizome section residue enzymatic activity | 100% | 32.5% | 40.6% | 18.5% |
| The dry matter content of lotus rhizome | 22.1% | 19.6% | 19.9% | 22.8% |
| Elasticity modulus/MPa of lotus rhizome block | 1.70 | 1.60 | 1.58 | 1.72 |
As can be seen from Table 2, after Low Temperature Plasma Treating, the remaining enzymatic activity of lotus rhizome section substantially reduces, explanation
Active ingredient in low temperature plasma is reacted with polyphenol oxidase, has brought it about inactivation, so as to delay polyphenol
Degraded of the oxidizing ferment to substrate aldehydes matter, has delayed the brown stain speed of lotus rhizome；The dry matter content of lotus rhizome does not have with elasticity modulus
Occur and be greatly lowered, illustrate that low temperature plasma does not have heavy damage lotus rhizome while preventing brown stain, prolonging storage period
Nutritional ingredient, this is probably since some amino acid in the active ingredient in low temperature plasma and lotus rhizome protein are sent out
Reaction is given birth to, especially, the dry matter content of lotus rhizome is not reduced with elasticity modulus in embodiment 3, illustrates the tertiary fourths of 2- hydroxyls -3-
The active ingredient accelerated in low temperature plasma of benzaldehyde and 3- (4- anisyls) -3- ethyl 3-oxopropanoates
With the reaction speed of polyphenol oxidase, so as to reduce degraded of the active ingredient to lotus rhizome protein.
By the lotus rhizome in embodiment 4 in 2- hydroxyls -3- tert-butyl benzenes formaldehyde and 3- (4- anisyls) -3- oxopropanoic acids
The processing of low temperature plasma is carried out under conditions of the different quality ratio of ethyl ester, by the ratio of both quality and lotus rhizome section
The remaining enzymatic activity of polyphenol oxidase is arranged to obtain Fig. 1.
By as shown in Figure 1, the remaining enzymatic activity and 2- hydroxyl -3- tert-butyl benzene formaldehyde of the polyphenol oxidase of lotus rhizome section
Have with the mass values of 3- (4- anisyls) -3- ethyl 3-oxopropanoates compared with Important Relations but linear dependence is not presented, work as 2-
The ratio of hydroxyl -3- tert-butyl benzenes formaldehyde and 3- (4- anisyls) -3- ethyl 3-oxopropanoates is between 3:1-9:When 1, lotus rhizome is cut
The remaining enzymatic activity of the polyphenol oxidase in face can as low as less than 17%, especially, when its ratio be 7:1 or between 4：1-5:1
When, the remaining enzymatic activity of polyphenol oxidase is down to less than 10%, can greatly strengthen degraded of the polyphenol oxidase to substrate,
So as to delay the brown stain of lotus rhizome.As seen from Figure 1, when 2- hydroxyls -3- tert-butyl benzenes formaldehyde and 3- (4- anisyls) -3-
Ethyl 3-oxopropanoate is in specific ratio, and the metallic plate coated with antioxidant anticorrosive coating passes through subacidity sodium chloride
After solution electrolyte soaks, for the reaction mechanism that certain is still not clear, low temperature plasma daughter is to the anti-of polyphenol oxidase
Answer speed to greatly improve, so as to reduce processing action time of the low temperature plasma daughter to lotus rhizome, also just reduce low temperature
Plasmon body to the palliating degradation degree of the nutriment such as protein of lotus rhizome, prevent-browning, it is fresh-keeping while, can be effective
Prevent the loss of lotus rhizome nutriment, therefore be a kind of new lotus rhizome prevent-browning preservation method efficiently, safe.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that described above
Specific embodiment only of the invention, is not intended to limit the invention, it is all done in the spirit of the present invention any repair
Change, supplement or similar fashion substitute etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of new lotus rhizome prevent-browning preservation method, including：The preparation of plasma, handle, prepare antistaling agent, post processing,
It is characterized in that：The processing step has on the metallic plate of one layer of antioxidant anticorrosive coating for lotus rhizome is placed in surface, room temperature
It is lower that metallic plate and lotus rhizome are immediately placed in sealable process chamber, the pressure of process chamber is evacuated to by 1-10Pa with vacuum pump, so
Injection cooling plasma, processing take out lotus rhizome after 7.5-10 minutes afterwards.
A kind of 2. new lotus rhizome prevent-browning preservation method according to claim 1, it is characterised in that：The plasma
Preparation process be：Using dielectric barrier discharge configuration device as plasma source, its frequency is 2.45-2.55GHz, power supply work(
Rate is 1.2-1.25kW, its sparking mode is igniting/pause：4-5s/6-7s, time cycle are 20-25 cycle, plasma
Humidified device is stand-by after being humidified with water.
A kind of 3. new lotus rhizome prevent-browning preservation method according to claim 1, it is characterised in that：The processing step
For：Not damaged, disease-free, without brown stain, without shrink fresh lotus rhizome are taken, and keeps rhizoma nelumbinis complete, is sterilized after cleaning with 2-40ppm
Water sterilization, lotus rhizome surface liquid is drained and is placed on metallic plate with holes, and there is one layer of antioxidant anticorrosive on the surface on metallic plate
Metallic plate and lotus rhizome, are immediately placed in sealable process chamber, are evacuated to the pressure of process chamber with vacuum pump by coating at room temperature
1-10Pa, is then injected into cooling plasma, and processing takes out lotus rhizome after 7.5-10 minutes.
A kind of 4. new lotus rhizome prevent-browning preservation method according to claim 3, it is characterised in that：The processing step
In antioxidant anticorrosive coating composition and its parts by weight be：80-100 parts of distilled water, 50-60 parts of aluminium dihydrogen phosphate, positive silicic acid
0.5-0.6 parts of ethyl ester, 0.8-1.0 parts of magnesia, 0.6-0.7 parts of zinc oxide, 2.4-2.5 parts of magnesium chromate, micro-nano aluminum powder 4.5-
4.8 parts, 0.05-0.06 parts of 3- (4- anisyls) -3- ethyl 3-oxopropanoates.
A kind of 5. new lotus rhizome prevent-browning preservation method according to claim 3, it is characterised in that：The processing step
In disinfectant be liquor natrii hypochloritis or sodium chloride solution or hydrogen peroxide solution or ClO 2 solution sodium chloride solution weak acid
Electrolyte.
A kind of 6. new lotus rhizome prevent-browning preservation method according to claim 1, it is characterised in that：Described preparing is fresh-keeping
Agent step is：0.15-0.2 parts of nisin, 0.3-0.5 parts of sodium phytate, 0.3-0.5 parts of sodium sorbate, plant is weighed to carry
3-6 parts of thing, 30-50 parts of distilled water is taken uniformly to mix 10-15 minutes at a temperature of 30-35 DEG C up to antistaling agent.
A kind of 7. new lotus rhizome prevent-browning preservation method according to claim 6, it is characterised in that：Described preparing is fresh-keeping
Plant extracts in agent step is：Flavone of hawthorn fruit insults jujube flavones or Rosmarinus officinalis extract or konjaku flour or grape pip extraction
Thing.
A kind of 8. new lotus rhizome prevent-browning preservation method according to claim 1, it is characterised in that：The post processing step
Suddenly it is：The submergence of the lotus rhizome of plasma-treated mistake is had in antistaling agent, frequency be 30-32kHz, power density 0.45-
0.55W/cm2Ultrasound condition under soak 2-3 minutes after take out, lotus rhizome is immediately placed in 5-7 DEG C of preservation.
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| CN110082301A (en) * | 2019-05-27 | 2019-08-02 | 武汉轻工大学 | A method of Different Harvesting Time lotus rhizome quality discrepancy is evaluated based on functional activity substance |
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