CN107950581A - A kind of composite microbial composition formula for preventing sweep stem nematode - Google Patents
A kind of composite microbial composition formula for preventing sweep stem nematode Download PDFInfo
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- CN107950581A CN107950581A CN201711339996.9A CN201711339996A CN107950581A CN 107950581 A CN107950581 A CN 107950581A CN 201711339996 A CN201711339996 A CN 201711339996A CN 107950581 A CN107950581 A CN 107950581A
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- dipel
- paecilomyces lilacinus
- brevibacillus laterosporus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The present invention provides it is a kind of prevent sweep stem nematode composite microbial composition formula, by weight, including：3 ~ 5 parts of Dipel, 2 ~ 4 parts of Brevibacillus laterosporus, 4 ~ 6 parts of Paecilomyces lilacinus.The present invention by Dipel, Brevibacillus laterosporus and Paecilomyces lilacinus bacterium powder compounding together with after, three shows significant synergistic function, compared to single Dipel, Brevibacillus laterosporus and Paecilomyces lilacinus bacterium powder, compounding microbial germ powder has for the preventive effect of sweet potato root knot nematode and very significantly improves；The problems such as solving the chemical pesticide longevity of residure length and biological agent slowly effect and unstable preventive effect of existing prevention sweep stem nematode at the same time.
Description
Technical field
The invention belongs to plant pest technical field of biological control, and in particular to a kind of to prevent answering for sweep stem nematode
Close anti-microbial formulation.
Background technology
Sweep stem nematode (Ditylenchus destructor Thorne) is a kind of destructive disease, is state's interplantation
One of thing quarantine object, after occurrence injury, influences sweet potato quality and yield huge.The underproduction 10%~50% can generally be caused,
It is serious to cause to have no harvest, it is one of three big Major Diseases of sweet potato.
Prevention for root-knot nematode, crop rotation are most important farming measures, effect also highly significant.But for more than people
Few area just seems especially difficult, has larger limitation.Second, the disease-resistant quality of plantation can resist stem to greatest extent
The harm of nematode, but due to being limited by various factors, Variety resistant to SCN selection and breeding and to promote difficulty larger, as conventional breeding due to
The characteristics of restriction of anti-source material scarcity, breeding cycle is long in addition, progress is very slow；And resisted using modern biotechnology
The work of nematodiasis breeding is still in the exploratory stage, wishes bright, Biological Control of Root-Knot Nematodes overview and progress, and 2003.Third, change
Learn nematicidal medicament to be prevented, the using effect " upright bar takes effect " of traditional fumigant and non-fumigant chemical agent, to line
The harm of parasitosis plays very big control action, and long-time service brings a series of problems, if pesticide residue is to people and other
The harm of biology, the pollution rampant again, to environment of Minor diseases etc. are increasingly prominent etc., and such medicament be mostly high poison, it is high residual
Pesticide is stayed, many of which is disabled successively.Paecilomyces lilacinus (Paecilomyces lilacinus (Thom) Samson) is made
For a kind of biological nematicidal bacterium, commercially produce and promoted and applied in sweet potato production, which has less toxic, pollution-free
The advantages that, but due to being influenced be subject to complicated soil variables in soil, there are slowly effect, preventive effect is unstable the deficiencies of it
Place, directly restricts large-scale promotion and application.
The content of the invention
To overcome the chemical pesticide longevity of residure length of existing prevention sweep stem nematode and biological agent slowly effect and preventive effect not
The problems such as stablizing, the present invention provides the composite microbial composition formula that a kind of quick longevity of residure is short and preventive effect is stablized, sweet to prevent
Potato haulm characters.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of composite microbial composition formula for preventing sweep stem nematode, by weight, including：3~5 parts of Dipel,
2~4 parts of Brevibacillus laterosporus, 4~6 parts of Paecilomyces lilacinus.
Preferably, the composite microbial composition formula, by weight, including：4~5 parts of Dipel, Brevibacillus laterosporus
3~4 parts, 5~6 parts of Paecilomyces lilacinus.
Compared with prior art, beneficial effects of the present invention are：
1st, the present invention by Dipel, Brevibacillus laterosporus and Paecilomyces lilacinus bacterium powder compounding together with after, three
Significant synergistic function is showed, compared to single Dipel, Brevibacillus laterosporus and Paecilomyces lilacinus
Powder, compounding microbial germ powder have for the preventive effect of sweet potato root knot nematode and very significantly improve.
2nd, the present invention compounds Dipel, Brevibacillus laterosporus and Paecilomyces lilacinus bacterium powder according to different numbers
Obtain the microbial germ powder of different ratio afterwards together, to the preventive effect of root-knot nematode there is also more significantly difference between them,
Wherein, 6 parts of 4 parts of Dipel, 3 parts of Brevibacillus laterosporus, Paecilomyces lilacinus combinations are compared to other combinations, and preventive effect is more
High and preventive effect is also more stablized.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and shortcoming will be with description and
It is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.People in the art
Member is it should be understood that without departing from the spirit and scope of the present invention can carry out the details and form of technical solution of the present invention
Modifications or substitutions, but these modifications and replacement are each fallen within protection scope of the present invention.
Dipel (Baecillus thuringiensis) bacterium powder of the present invention is purchased from Hubei agriculture section paddy Eco Science Technology
Co., Ltd, containing spore >=10,000,000,000/g living；Brevibacillus laterosporus (Brevibacillus laterosporu) bacterium powder is purchased from
Guangzhou agriculture hat (Taiwan) bio tech ltd production, containing spore >=10,000,000,000/g living；Paecilomyces lilacinus
(Paecilomyces lilacinus) bacterium powder is produced purchased from Zhengzhou Le Beifeng bio tech ltd, containing spore >=100 living
Hundred million/g.
Embodiment 1
Mix, obtain compound by 3 parts of Dipel, 2 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 2
Mix, obtain compound by 3 parts of Dipel, 2 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 3
Mix, obtain compound by 3 parts of Dipel, 2 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 4
Mix, obtain compound by 3 parts of Dipel, 3 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 5
Mix, obtain compound by 3 parts of Dipel, 3 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 6
Mix, obtain compound by 3 parts of Dipel, 3 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 7
Mix, obtain compound by 3 parts of Dipel, 4 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 8
Mix, obtain compound by 3 parts of Dipel, 4 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 9
Mix, obtain compound by 3 parts of Dipel, 4 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 10
Mix, obtain compound by 4 parts of Dipel, 2 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 11
Mix, obtain compound by 4 parts of Dipel, 2 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 12
Mix, obtain compound by 4 parts of Dipel, 2 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 13
Mix, obtain compound by 4 parts of Dipel, 3 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 14
Mix, obtain compound by 4 parts of Dipel, 3 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 15
Mix, obtain compound by 4 parts of Dipel, 3 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 16
Mix, obtain compound by 4 parts of Dipel, 4 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 17
Mix, obtain compound by 4 parts of Dipel, 4 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 18
Mix, obtain compound by 4 parts of Dipel, 4 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 19
Mix, obtain compound by 5 parts of Dipel, 2 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 20
Mix, obtain compound by 5 parts of Dipel, 2 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 21
Mix, obtain compound by 5 parts of Dipel, 2 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 22
Mix, obtain compound by 5 parts of Dipel, 3 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 23
Mix, obtain compound by 5 parts of Dipel, 3 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 24
Mix, obtain compound by 5 parts of Dipel, 3 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 25
Mix, obtain compound by 5 parts of Dipel, 4 parts of Brevibacillus laterosporus, 4 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 26
Mix, obtain compound by 5 parts of Dipel, 4 parts of Brevibacillus laterosporus, 5 parts of Paecilomyces lilacinus
Microbial bacterial agent.
Embodiment 27
Mix, obtain compound by 5 parts of Dipel, 4 parts of Brevibacillus laterosporus, 6 parts of Paecilomyces lilacinus
Microbial bacterial agent.
The field trial of composite microbial composition formula prevention sweep stem nematode of the present invention
1st, materials and methods
1.1 experimental field select：Experimental field it is selected in Dengfeng City to accomplish three Zu Lixiao armies farmland covered by contract of town tea-booth ditch village, area 7.5
Mu；Sweet potato continuous cropping is planted 8 years, and field seriously occurs for sweep stem nematode.
1.2 experimental cultivar：Beijing 553 (for the high sense kind of sweep stem nematode).
1.3 for examination microbial product：Bacillus thuringiensispowder, containing spore >=10,000,000,000/g living；Brevibacillus laterosporus bacterium
Powder, containing spore >=10,000,000,000/g living；Paecilomyces lilacinus bacterium powder, spore >=10,000,000,000/g living.
2nd, test method design
2.1 experimental design
(1) test organisms product Dipel, Brevibacillus laterosporus, Paecilomyces lilacinus different ratio prevention Sweet Potato line
Worm effect test：Using comprehensive test method (consideration interaction), if Dipel, Brevibacillus laterosporus, Paecilomyces lilacinus 3
A factor, per factor set Dipel 3,4,5 parts, Brevibacillus laterosporus 2,3,4 parts, Paecilomyces lilacinus 4,5,6 part each 3
A level, is shown in Table 1.Utilize comprehensive test (33) be designed, totally 27 handle the (microorganism with 1~27 compound of embodiment
Bacterium product are completely the same, and numbering is for trying bacterium product 1~27 respectively in order).
The experimental design table of the present invention of table 1
(2) Dipel, Brevibacillus laterosporus, Paecilomyces lilacinus optimal proportion and Dipel, side spore are short
Bacillus, Paecilomyces lilacinus single dose prevention sweet potato stem nematode effect test, experiment set 5 processing.
Processing 1：Preferable Dipel, Brevibacillus laterosporus, Paecilomyces lilacinus optimal proportion compound bacterium product；
Processing 2：Compare bacterium product 1：Bacillus thuringiensispowder, containing spore >=10,000,000,000/g living；
Processing 3：Compare bacterium product 2：Brevibacillus laterosporus bacterium powder, containing spore >=10,000,000,000/g living；
Processing 4：Compare bacterium product 3：Paecilomyces lilacinus bacterium powder, containing spore >=10,000,000,000/g living；
Processing 5：Blank control：The medicament of any prevention nematode is not applied.
Design 2.2 experimental plots：Cell length and width each 10m, area 100m2, line-spacing 50cm, spacing in the rows 30cm；Repeat three times.
3rd, method for planting
Potato seedling growth is handled before 3.1 cultivations：To ensure plant potato seedling growth not disease carrying germ, sweet potato seedling is used on sweet potato seedbed from bed surface 5
Cutting seedlings at~10cm, by potato seedling growth lower semisection 5cm immerse 46~48 DEG C of hot water in 10 minutes, after be put into cold water rinse after plant.
3.2 application method
By every 667m2Mixed with composite bacteria agent 5kg refinement soil 18kg, when sweet potato is transplanted, by mixed bacterium soil after pit digging
Apply in cave per cave 10g, and mixed with the soil in cave, then sweet potato seedling is loaded into cave.
4th, effect analysis method
4.1 morbidity potato wedge grade scales
During harvest in units of cell, potato wedge is classified by morbidity weight, is weighed respectively.Severity Scaling standard is：
0 grade：Potato wedge disease-free spot；1 grade：Potato wedge lesion accounts for full potato wedge below 1/4；2 grades：Potato wedge lesion account for full potato wedge 1/4~
1/2；3 grades：Potato wedge lesion accounts for full potato wedge 1/2~3/4；4 grades：Potato wedge lesion accounts for full potato wedge more than 3/4.
4.2 drug effect computational methods
Disease index (%)=(∑ (each disease level block number × disease level value)/(investigation total block data × highest disease level value)) ×
100
Prevention effect (%)=((check plot disease refers to-prevents area's disease and refers to)/check plot disease refers to) × 100
Rate of growth (%)=((check plot yield-prevention area yield)/check plot yield) × 100
5th, result and analysis
5.1 Dipels, Brevibacillus laterosporus, Paecilomyces lilacinus different ratio microbial inoculum prevention sweet potato stem nematode effect
Fruit (is shown in Table 1).
1 different ratio microbial inoculum of table prevents sweet potato stem nematode effect
As it can be seen from table 1 to each factor varying level yield (i.e. K values) be compared understand, Dipel because
In element, 2 (K of Dipel2=149071) 3 (K of ＞ Dipels3=148813) 1 (K of ＞ Dipels1=
113543)；In Brevibacillus laterosporus, 2 (K of Brevibacillus laterosporus2=151244) 3 (K of ＞ Brevibacillus laterosporus3=
146978) 1 (K of ＞ Brevibacillus laterosporus1=145833)；In Paecilomyces lilacinus, 3 (K of Paecilomyces lilacinus3=
152923) 2 (K of ＞ Paecilomyces lilacinus2=147185) 1 (K of ＞ Paecilomyces lilacinus1=143947).
Drawn from three kinds of Dipel, Brevibacillus laterosporus, Paecilomyces lilacinus element interaction comparative results
Optimum combination is：Dipel 2 (4 parts), Brevibacillus laterosporus 2 (3 parts), Paecilomyces lilacinus 3 (6 parts), and the combination
The 15th processing combination (i.e. embodiment 15) exactly in comprehensive test, yield also highest, is 1771kg/hm2.It is therefore seen that point
Analyse result and result of the test is completely the same.Show under this experimental condition, 4 parts of Dipel, 3 parts of Brevibacillus laterosporus,
6 parts of Paecilomyces lilacinus combines for optimal proportion.
5.2 Dipels, Brevibacillus laterosporus, Paecilomyces lilacinus optimal proportion and Dipel, side spore are short
Bacillus, Paecilomyces lilacinus single dose prevention sweet potato stem nematode effect test (being shown in Table 2).
The preferred tri compound bacterium product of table 2 and Dipel, Brevibacillus laterosporus, the prevention of Paecilomyces lilacinus single dose are sweet
Potato Ditylenchus dipsaci effect
From table 2 it can be seen that tri compound bacterium product are to prevention sweet potato stem nematode effect, to be above Dipel, side spore short
Bacillus and Paecilomyces lilacinus single dose, respectively it is high by 27.87%, 19.58% and 7.99%.
According to the test data in table 2, using average disease index as parameter, test to result of the test, row variance point
Analyse table (table 3).
3 result of the test analysis of variance table of table
By table 3 as it can be seen that in 4 kinds of microbial bacterias used, to the prevention effect of sweet potato stem nematode, between microbial inoculum, difference is very
Significantly.
Claims (2)
- A kind of 1. composite microbial composition formula for preventing sweep stem nematode, it is characterised in that by weight, including：Su Yun gold bars 3 ~ 5 parts of bacterium, 2 ~ 4 parts of Brevibacillus laterosporus, 4 ~ 6 parts of Paecilomyces lilacinus.
- 2. a kind of composite microbial composition formula for preventing sweep stem nematode according to claim 1, it is characterised in that described Composite microbial composition formula, by weight, including：4 ~ 5 parts of Dipel, 3 ~ 4 parts of Brevibacillus laterosporus, Paecilomyces lilacinus 5 ~ 6 parts.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717725A (en) * | 2021-10-13 | 2021-11-30 | 辽宁省农业科学院 | Microbial agent |
| CN114455985A (en) * | 2022-02-14 | 2022-05-10 | 云南省农业科学院农业环境资源研究所 | Preparation method of compound biological agent for preventing and treating aucklandia root rot and nematode disease |
| WO2024082036A1 (en) * | 2022-10-21 | 2024-04-25 | Total Biotecnologia Indústria E Comercio S.A. | Agricultural bioinsecticide composition containing brevibacillus laterosporus, different subspecies of bacillus thuringiensis and botanical extracts of citronella and melaleuca, industrial process and use of same on crops of agricultural importance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103918718A (en) * | 2013-01-14 | 2014-07-16 | 陕西汤普森生物科技有限公司 | Composition containing brevibacillus laterosporus |
| CN105076217A (en) * | 2014-05-20 | 2015-11-25 | 华中农业大学 | Method and device for preparing Paecilomyces lilacinus fungicide |
| CN105163590A (en) * | 2012-12-03 | 2015-12-16 | 拜耳作物科学股份公司 | Composition comprising biological control agents |
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2017
- 2017-12-14 CN CN201711339996.9A patent/CN107950581A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105163590A (en) * | 2012-12-03 | 2015-12-16 | 拜耳作物科学股份公司 | Composition comprising biological control agents |
| CN103918718A (en) * | 2013-01-14 | 2014-07-16 | 陕西汤普森生物科技有限公司 | Composition containing brevibacillus laterosporus |
| CN105076217A (en) * | 2014-05-20 | 2015-11-25 | 华中农业大学 | Method and device for preparing Paecilomyces lilacinus fungicide |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717725A (en) * | 2021-10-13 | 2021-11-30 | 辽宁省农业科学院 | Microbial agent |
| CN114455985A (en) * | 2022-02-14 | 2022-05-10 | 云南省农业科学院农业环境资源研究所 | Preparation method of compound biological agent for preventing and treating aucklandia root rot and nematode disease |
| WO2024082036A1 (en) * | 2022-10-21 | 2024-04-25 | Total Biotecnologia Indústria E Comercio S.A. | Agricultural bioinsecticide composition containing brevibacillus laterosporus, different subspecies of bacillus thuringiensis and botanical extracts of citronella and melaleuca, industrial process and use of same on crops of agricultural importance |
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Application publication date: 20180424 |