CN1079430C

CN1079430C - High-pressure gas gene gun

Info

Publication number: CN1079430C
Application number: CN99124246A
Authority: CN
Inventors: 周芳
Original assignee: Individual
Current assignee: NINGBO SCIENTZ BIOTECHNOLOGY CO Ltd
Priority date: 1999-12-11
Filing date: 1999-12-11
Publication date: 2002-02-20
Anticipated expiration: 2019-12-11
Also published as: CN1262323A

Abstract

The utility model provides a high-pressure gas gene rifle, including shell and the compressed gas intake pipe (2) that is located it, launch the chamber, the bombardment chamber, launch the intracavity from last to including gas storage bin (3) down, but rupture membrane (7), bullet seat (6), bullet (8) and bullet dog (11), when the inner chamber of gas storage bin (3) is aerifyd to the burst pressure of rupture membrane 7, but rupture membrane 7 breaks, produce a gas shock wave, push bullet (8) to bullet dog (11) and be blocked by it with higher speed, the particle 24 that scribbles on bullet (8) surface this moment is through bullet dog (11) centre bore high-speed flight and jet into in the sample to accomplish the gene and lead in the experiment. The invention has the advantages of no impurity, good stability, high efficiency and high initial speed of the DNA bullet.

Description

A kind of high pressure gas particle gun

The present invention relates to a kind ofly genetic material or other materials be injected the instrument of cell, tissue or organoid, particularly a kind of high pressure gas particle gun in bio-genetics.

In the bio-genetics field, particle gun is a kind of requisite instrument, initial particle gun is to adopt the gunpowder bullet to bombard, as the patent No. is that 89109334 Chinese invention patent " a kind of method of metastatic gene and the particle gun of metastatic gene thereof " discloses the particle gun so a kind of, it utilizes " speed and the method for the proportion speed ground control bombardment of gunpowder; also can operate under the closed state He under the non-closed state with task equipment; both can bombard also and can bombard the importing that realizes foreign gene to the substance biomass cells the biomass cells that exsomatizes, thereby change biological proterties." this genoid rifle can damage to some extent to sample, the impurity in the gunpowder also can be injected in the sample simultaneously, and its precision often is difficult to control.

Purpose of the present invention utilizes gas percussion ripple that pressurized gas converts to as propulsion source and the high desk-top particle gun of DNA muzzle velocity with regard to being to provide at above-mentioned prior art present situation a kind of.

The object of the present invention is achieved like this: the high pressure gas particle gun comprises shell and is positioned at its pressurized gas inlet pipe, emission cavity, bombardment chamber; Emission cavity is by loam cake, pillar buoy I and chassis I surround, include the gas storage storehouse in it from top to bottom, but fracturing diaphragm, the bullet seat, bullet and bullet block, having ventilating pit on the I of chassis is connected with the bombardment chamber, gas storage storehouse and loam cake fix, its inner chamber is connected with inlet pipe, but the bottom is then sealed by fracturing diaphragm, the bullet seat is fixed on the bottom in gas storage storehouse, its centre hole is relative with the inner chamber in gas storage storehouse, the bullet that section is the font of falling T is filled in the centre hole bottom at the bullet seat from bottom to top, and the bullet block that can hold the bullet after the emission is positioned under the bullet and embeds on the I of chassis; The bombardment chamber is then surrounded by chassis I, the pillar buoy II and the chassis II that send out the chamber other, is provided with a specimen holder in the chamber.

During use, planchet is placed on the specimen holder of bombardment in the chamber, just is placed on the central authorities of planchet and aims at the centre hole of bullet block, then be coated on the font of falling T bullet lower surface as the particulate that is coated with DNA of bullet as the sample of target.

Use for convenience, also unexpectedly excessive in order to prevent to bombard cavity pressure, under described bombardment chamber, also can be provided with the snubber assembly that constitutes by supporting plate, support spring and housing.

Compare with existing, the present invention adopts the gas percussion ripple that is converted to by pressurized gas as propulsion source, thereby do not have doping impurity wherein, good stability, efficient height, and because the bump of bullet and bullet block is an inflexible, thereby the DNA bullet can obtain more energy, thereby obtains higher initial velocity.

Description of drawings:

Fig. 1 is the structure sectional view of the embodiment of the invention.

Emission cavity when Fig. 2 does not launch as yet for bullet and the partial enlarged drawing in bombardment chamber

Fig. 3 is the partial enlarged drawing of the emission cavity after the bullet emission with the bombardment chamber

Embodiment is described in further detail the present invention below in conjunction with accompanying drawing.

As Fig. 1～shown in Figure 3, the high pressure gas particle gun comprises shell and is positioned at its pressurized gas inlet pipe 2, emission cavity, bombardment chamber;

Shell is made of upper housing 23 and lower housing 21 and base 20, and the front of lower housing 21 has window or wicket with the convenient (not shown) of using;

Pressurized gas inlet pipe 2 one ends are connected with source of the gas, and gas storage storehouse 3 intracavity inter-connections of an end and emission cavity, source of the gas can adopt the compressed nitrogen or the compressed helium of steel cylinder dress, because of the expense economy of nitrogen and can meet the demands, generally adopt nitrogen;

Emission cavity is surrounded by loam cake 4, pillar buoy I5 and chassis I9, one extraction pipe 22 is fixed on the loam cake 4 and with cavity and communicates, having ventilating pit 10 on the I9 of chassis is connected with the bombardment chamber, but include gas storage storehouse 3 fracturing diaphragms 7, bullet seat 6, bullet 8 and bullet block 11 in the emission cavity from top to bottom: gas storage storehouse 3 is fixed on the loam cake 4, its inner chamber is connected with inlet pipe 2, but the bottom is then sealed by fracturing diaphragm 7, be more convenient use, the present invention also connects by a tensimeter 1 with its intracavity inter-connection in the upper end in gas storage storehouse, so that the pressure in the Real Time Observation gas storage storehouse 3; Bullet seat 6 is the bottom that a nut is fixed on gas storage storehouse 3, the inner chamber in its centre hole and gas storage storehouse 3 is to directly, section is the bullet 8 of the font of falling T and fills in from bottom to top bottom the centre hole of bullet seat 6, bullet block 11 is positioned under the bullet 8 and embeds middle position at chassis I9, can hold and stop bullet 8 after bullet 8 emissions;

The bombardment chamber is then surrounded by chassis I9, pillar buoy II12 and the chassis II16 of emission cavity, planchet 15 is placed on the specimen holder 14 that bombards in the chamber, 14 of specimen holders are put on the convex tendon 13 that is placed on pillar buoy II12, and just be placed in the planchet 15, and aim at bullet block 11 centre holes of emission cavity as the sample of target; According to difference highly multiple tracks convex tendon 13 can be set on pillar buoy II12, so that select the placement height of planchet 15;

In the present embodiment, under the bombardment chamber, also be provided with the snubber assembly that constitutes by supporting plate 17, support spring 18 and housing 19 thereof, the chassis II16 in bombardment chamber promptly is placed on the supporting plate 17, the effect of snubber assembly has two: one, can depress supporting plate 17 in use, to make things convenient for the installation of pillar buoy II12, the 2nd, when the pressure in the bombardment chamber is unexpectedly excessive, spring 18 is compressed and pillar buoy 12 is moved down and breaks away from the chassis I9 of emission cavity, thereby the gas in the bombardment chamber is overflowed and pressure release, to guarantee safety.

The present invention in use, but at first select the fracturing diaphragm 7 of required pressure intensity, it is inserted on the step of bullet seat 6 endoporus, bullet seat 6 is rotated with in 3 bottoms, gas storage storehouse; At the particulate 24 of the font of falling T bullet 8 lower surfaces coating coating by DNA, and in the centre hole with its insertion bullet seat 6; Select suitable height print frame 14 to be rested on the convex tendon 13 of pillar buoy 12, put on the specimen holder 14 in bombardment chamber with being equipped with by the planchet 15 of bombardment sample and aim at bullet block 11;

Start vacuum pump and be evacuated to suitable pressure (being generally 0.085-0.095Mpa) by 22 pairs of emission cavities of extraction pipe and bombardment chamber, inflate to the inner chamber in gas storage storehouse 3 through inlet pipe 2 by high-pressure air source again, but when reaching the burstpressures of fracturing diaphragm 7, but fracturing diaphragm 7 breaks, produce a gas percussion ripple, bullet 8 is quickened to push bullet block 11 to and blocked by it, be coated in bullet 8 lip-deep particulates 24 this moment promptly by the 11 centre hole high-speed flights of bullet block and inject in the sample, a gene imports test and finishes.

Claims

1. high pressure gas particle gun, comprise shell and be positioned at its pressurized gas inlet pipe (2), emission cavity, the bombardment chamber, it is characterized in that emission cavity is by loam cake (4), pillar buoy I (5) and chassis I (9) surround, include gas storage storehouse (3) in it from top to bottom, but fracturing diaphragm (7), bullet seat (6), bullet (8) and bullet block (11), having ventilating pit (10) on the chassis I (9) is connected with the bombardment chamber, gas storage storehouse (3) fixes with loam cake (4), its inner chamber is connected with inlet pipe (2), but the bottom is then sealed by fracturing diaphragm (7), bullet seat (6) is fixed on the bottom of gas storage storehouse (3), its centre hole is relative with the inner chamber of gas storage storehouse (3), section is the bullet (8) of the font of falling T to be filled in from bottom to top bottom the centre hole of bullet seat (6), and the bullet block (11) of the bullet after can holding and launch is positioned under the bullet (8) and embeds on chassis I (9); The bombardment chamber is then surrounded by chassis I (9), pillar buoy II (12) and the chassis II (16) of emission cavity, is provided with a specimen holder (14) in the chamber.

2. high pressure gas particle gun as claimed in claim 1, it is characterized in that also being provided with under described bombardment chamber the snubber assembly by supporting plate (17) and support spring (18) and housing (19) formation, the chassis II (16) in bombardment chamber is placed on the supporting plate (17).

3. high pressure gas particle gun as claimed in claim 1 or 2 is characterized in that described specimen holder (14) puts on the convex tendon (13) that is placed on pillar buoy II (12).

4. high pressure gas particle gun as claimed in claim 1 or 2, the upper end that it is characterized in that gas storage storehouse (3) is connected with the tensimeter (1) with its intracavity inter-connection.