CN107941772A - Illuminate monitoring system and vegetation monitoring device - Google Patents
Illuminate monitoring system and vegetation monitoring device Download PDFInfo
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- CN107941772A CN107941772A CN201711368482.6A CN201711368482A CN107941772A CN 107941772 A CN107941772 A CN 107941772A CN 201711368482 A CN201711368482 A CN 201711368482A CN 107941772 A CN107941772 A CN 107941772A
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- sample
- led light
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- light ring
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- 238000012544 monitoring process Methods 0.000 title claims abstract description 62
- 238000012806 monitoring device Methods 0.000 title claims abstract description 15
- 238000003384 imaging method Methods 0.000 claims abstract description 47
- 238000005286 illumination Methods 0.000 claims abstract description 35
- 230000013011 mating Effects 0.000 claims abstract description 27
- 239000000523 sample Substances 0.000 claims description 106
- 230000008859 change Effects 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 1
- 241000252212 Danio rerio Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Studio Devices (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to one kind to illuminate monitoring system, including：Lighting device, mating plate imaging device, sample position monitoring camera and computer；The lighting device includes a LED light ring and light source controller, and the LED light ring is placed in the side of sample cell, and the lighting device provides illumination and temperature for sample grown and imaging unit imaging；The mating plate imaging device is located at the sample cell side opposite with the LED light ring, and the mating plate imaging device includes an image-forming objective lens；Sample position monitoring camera is located in the ring of the LED light ring, for monitoring the position of sample；The computer monitors phase mechatronics with the lighting device, mating plate imaging device and sample position.A kind of vegetation monitoring device is also provided.Above-mentioned illumination monitoring system and vegetation monitoring device can provide illumination, convenient operation, and improve efficiency and precision.
Description
Technical field
The invention belongs to mating plate to illuminate imaging technique, more particularly to a kind of illumination monitoring system and vegetation monitoring dress
Put.
Background technology
Mating plate illumination imaging (light sheet illumination) technology, is also named selection flat illumination imaging
(selective plan illumination) is a kind of in life science, carrying out larger tissue block and pattern life
Thing (such as zebra fish, nematode) carries out the mode of fluorescence imaging.Mating plate imaging be exactly pass through will excite illumination light with perpendicular into
As direction (rather than wide field or co-focusing imaging excite like that be imaged it is coaxial) layer of irradiating sample as thin slice
Face, and the fluorescence that the aspect is excited is imaged.Mating plate be imaged on progress it is big/thick tissue, embryo, model animal imaging when,
There are a variety of advantages compared to more traditional co-focusing imaging：1. lower fluorescent bleach；2. lower sample damage；3. can be into
Row high speed high s/n ratio is imaged；4. without pin hole, therefore the sensitivity with higher, and can realize the sample of multi-angle into
Picture.
Main target is positioned as model animal by current mating plate imaging product, while carries out 37 degree to sample area
Temperature control, maintains the normal activity of model animal and is observed.For model plant (or other need the biology of illumination),
Illumination in growth course is indispensable.
The content of the invention
Based on this, it is necessary to provide a kind of illumination monitoring system that can be provided illumination and monitor biological growth and plant life
Long monitoring device.
One kind illumination monitoring system, including：Lighting device, mating plate imaging device, sample position monitoring camera and computer；
The lighting device includes a LED light ring and light source controller, and the LED light ring is placed in the side of sample cell, institute
State lighting device and provide illumination for sample grown and mating plate imaging device imaging；
The mating plate imaging device is located at the sample cell side opposite with the LED light ring, the mating plate imaging device bag
Include an image-forming objective lens；
The sample position monitoring camera is located in the ring of the LED light ring, for monitoring the position of sample；
The computer and the lighting device, the mating plate imaging device, sample position monitoring phase mechatronics.
In one of the embodiments, the LED light ring is full-color LED lamp ring, and the light source control is controlled by computer
Device processed regulates and controls sunlit relative luminance change and colour temperature change during the LED light ring moulds are intended one day.
In one of the embodiments, the sample position monitoring camera includes sample position monitoring probe, the sample
Position monitoring probe is positioned at the center of the LED light ring.
In one of the embodiments, the camera lens of the sample position monitoring camera is wide-angle lens.
In one of the embodiments, the sample position monitor camera in the range of 0.5cmx0.5cm~5cmx5cm into
Picture, observes according to the areas imaging of sample position monitoring camera and adjusts the position of the sample.
A kind of vegetation monitoring device, including monitoring system and sample cell are illuminated as described above, in the sample cell
Sample be plant.
In one of the embodiments, the LED light ring is full-color LED lamp ring, and the light source control is controlled by computer
Device processed regulates and controls sunlit relative luminance change and colour temperature change during the LED light ring moulds are intended one day.
In one of the embodiments, the sample position monitoring camera includes sample position monitoring probe, the sample
Position monitoring probe is positioned at the center of the LED light ring.
In one of the embodiments, the camera lens of the sample position monitoring camera is wide-angle lens.
In one of the embodiments, the sample position monitor camera in the range of 0.5cmx0.5cm~5cmx5cm into
Picture, observes according to the areas imaging of sample position monitoring camera and adjusts the position of the sample.
In above-mentioned illumination monitors system and vegetation monitoring device, LED light ring is set in the side of sample cell, is mould
Formula plant or other need the biological long-time growth of illumination and mating plate illumination imaging to provide support.Set among annular LED
The sample position detection camera put can be imaged in the range of Centimeter Level, and camera, observer are detected by the sample position
It is capable of the general placement location of close-ups sample, is moved to the object for needing to observe by the operation in computer software
Before image-forming objective lens camera lens.It is not required observer to lie prone in sample trough rim, is observed with eyes and judge small sample, convenient operation,
And improve efficiency and precision.
Brief description of the drawings
Fig. 1 is the structure diagram of the illumination monitoring system of an embodiment；
Fig. 2 is the structure diagram of the vegetation monitoring device of an embodiment.
Embodiment
Referring to Fig. 1, the illumination monitoring system 100 of an embodiment, including：Lighting device 10, mating plate imaging device 20,
Sample position monitors camera 30 and computer (not shown).
In one embodiment, lighting device 10 includes a LED light ring 110 and light source controller (not shown), LED light ring
110 are placed in the side of sample cell, and LED light ring 110 includes multiple LED lamp sources, and lighting device 10 is sample grown and the imaging
Cell imaging provides illumination and temperature, and light source controller is used for the setting for controlling LED light ring.Mating plate imaging device 20 is located at sample
The groove side opposite with LED light ring 10, including an image-forming objective lens 210.Sample position monitoring camera 30 is located at the ring of LED light ring 10
It is interior, for monitoring the position of sample；Computer monitors camera with lighting device 10, mating plate imaging device 20, sample position respectively
30 are electrically connected, and collect the data message of sample position monitoring camera 30, and control lighting device 10 and mating plate imaging device 20
Parameter setting and regulation and control.
In above-mentioned illumination monitoring system, the setting LED light ring 110 in the side of sample cell, is model plant or other are needed
The biological long-time growth and mating plate illumination imaging for wanting illumination provide support.The sample position set among annular LED 110
Detection camera 30 can be imaged in the range of Centimeter Level, detect camera 30 by the sample position, observer being capable of low coverage
From the general placement location of observation sample, the object for needing to observe is moved to by image-forming objective lens by the operation in computer software
Before 210 camera lenses.It is not required observer to lie prone in sample trough rim, is observed with eyes and judge small sample, convenient operation, and improve
Efficiency and precision.
In one embodiment, LED light ring 110 is full-color LED lamp ring, regulates and controls control light source controller from computer terminal,
And then LED light ring 110 is set to simulate sunlit relative luminance change and colour temperature change in one day.
In one embodiment, sample position monitoring camera 30 includes sample position monitoring probe 310, sample position monitoring
Probe is positioned at the center of LED light ring 110.In one embodiment, the camera lens of sample position monitoring camera 30 is wide-angle lens
Head.Sample position monitoring camera 30 can be imaged in the range of 0.5cmx0.5cm~5cmx5cm, according to the areas imaging, be calculated
Machine is observed according to the areas imaging of sample position monitoring camera 30 and adjusts the position of sample.Compared in mating plate imaging process,
Since the image-forming objective lens of use are different, its areas imaging is different, and areas imaging is 0.22mmx0.22mm to 2.2mmx2.2mm, because
This must accurately adjust sample and scope very small before hard constraints to imaging lens, is only possible to successfully be imaged.According to upper
State description to understand, since image-forming objective lens areas imaging is minimum, be only capable of carrying out in the areas imaging of its acquisition fine being adjusted to picture
Scope.Camera 30 is monitored by above-mentioned sample position, observer is capable of the general placement location of close-ups sample, Neng Goufang
Just the object observed will be needed to be moved to image-forming objective lens camera lens by the operation in computer software before.So there is no need to see
Survey person lies prone in sample trough rim, is observed with eyes and judges small sample, adjusted sample position, improve efficiency and precision.
Referring to Fig. 2, the vegetation monitoring device 200 of an embodiment, including illumination monitoring system as described above
100 and sample cell 120, specifically, the sample in sample cell 120 is plant, such as arabidopsis.In one embodiment, sample cell
120 include sample holder 122, are used to support sample.
The concrete structure of illumination monitoring system 100 is identical with foregoing teachings, and details are not described herein.
In above-mentioned vegetation monitoring device, LED light ring is set in the side of sample cell, is model plant or other need
The biological long-time growth and mating plate illumination imaging for wanting illumination provide support.The sample position inspection set among annular LED
Surveying camera can be imaged in the range of Centimeter Level, detect camera by the sample position, observer being capable of close-ups
The general placement location of sample, image-forming objective lens camera lens is moved to by the operation in computer software by the object for needing to observe
Before.Be not required observer to lie prone in sample trough rim, observed with eyes and judge small sample, convenient operation, and improve efficiency and
Precision.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope that this specification is recorded all is considered to be.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Cannot therefore it be construed as limiting the scope of the patent.It should be pointed out that come for those of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. one kind illumination monitoring system, it is characterised in that including：Lighting device, mating plate imaging device, sample position monitoring camera
And computer；
The lighting device includes a LED light ring and light source controller, and the LED light ring is placed in the side of sample cell, the photograph
Bright device provides illumination for sample grown and mating plate imaging device imaging；
The mating plate imaging device is located at the sample cell side opposite with the LED light ring, and the mating plate imaging device includes one
Image-forming objective lens；
The sample position monitoring camera is located in the ring of the LED light ring, for monitoring the position of sample；
The computer and the lighting device, the mating plate imaging device, sample position monitoring phase mechatronics.
2. illumination monitoring system according to claim 1, it is characterised in that the LED light ring is full-color LED lamp ring, is led to
Crossing computer controls the light source controller to regulate and control sunlit relative luminance change and color during the LED light ring moulds are intended one day
Temperature change.
3. illumination monitoring system according to claim 1, it is characterised in that the sample position monitoring camera includes sample
Position monitoring is popped one's head in, and the sample position monitoring probe is positioned at the center of the LED light ring.
4. illumination monitoring system according to claim 1, it is characterised in that the camera lens of sample position monitoring camera is
Wide-angle lens.
5. illumination monitoring system according to claim 1, it is characterised in that the sample position monitoring camera exists
It is imaged in the range of 0.5cmx0.5cm~5cmx5cm, is observed according to the areas imaging of sample position monitoring camera and adjust institute
State the position of sample.
6. a kind of vegetation monitoring device, it is characterised in that including the illumination monitoring system and sample described in claim 1
Groove, the sample in the sample cell is plant.
7. vegetation monitoring device according to claim 6, it is characterised in that the LED light ring is full-color LED lamp
Ring, controls the light source controller to regulate and control sunlit relative luminance during the LED light ring moulds are intended one day and becomes by computer
Change and colour temperature changes.
8. vegetation monitoring device according to claim 6, it is characterised in that the sample position monitoring camera includes
Sample position monitoring probe, the sample position monitoring probe is positioned at the center of the LED light ring.
9. vegetation monitoring device according to claim 6, it is characterised in that the mirror of the sample position monitoring camera
Head is wide-angle lens.
10. vegetation monitoring device according to claim 6, it is characterised in that the sample position monitoring camera exists
It is imaged in the range of 0.5cmx0.5cm~5cmx5cm, is observed according to the areas imaging of sample position monitoring camera and adjust institute
State the position of sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711368482.6A CN107941772A (en) | 2017-12-18 | 2017-12-18 | Illuminate monitoring system and vegetation monitoring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711368482.6A CN107941772A (en) | 2017-12-18 | 2017-12-18 | Illuminate monitoring system and vegetation monitoring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107941772A true CN107941772A (en) | 2018-04-20 |
Family
ID=61940681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711368482.6A Pending CN107941772A (en) | 2017-12-18 | 2017-12-18 | Illuminate monitoring system and vegetation monitoring device |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101285564A (en) * | 2008-06-13 | 2008-10-15 | 中国农业科学院农业环境与可持发展研究所 | Solar energy driven plant growth lamp |
| CN103091296A (en) * | 2013-01-23 | 2013-05-08 | 浙江大学 | Plant physical condition detection method based on spectral imaging technology, and device for same |
| CN103826437A (en) * | 2011-07-27 | 2014-05-28 | 陶氏益农公司 | Plant growth kinetics captured by motion tracking |
| CN204026228U (en) * | 2014-07-09 | 2014-12-17 | 宁波米德兰电子制造有限公司 | LED plant growth lamp |
| CN104807761A (en) * | 2015-05-08 | 2015-07-29 | 南开大学 | Design method of spectrograph for realizing micro-area spectral measurement |
| CN105651785A (en) * | 2015-12-31 | 2016-06-08 | 中国科学院半导体研究所 | Microscopic imaging device and method for measuring microstructure defects on surface of semiconductor material |
| CN106922414A (en) * | 2017-02-27 | 2017-07-07 | 广东工业大学 | A kind of intelligent controlling device and method that plant growth lighting is carried out with LED |
| CN207650107U (en) * | 2017-12-18 | 2018-07-24 | 北京赛尔怡美科技有限公司 | Illuminate monitoring system and vegetation monitoring device |
-
2017
- 2017-12-18 CN CN201711368482.6A patent/CN107941772A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101285564A (en) * | 2008-06-13 | 2008-10-15 | 中国农业科学院农业环境与可持发展研究所 | Solar energy driven plant growth lamp |
| CN103826437A (en) * | 2011-07-27 | 2014-05-28 | 陶氏益农公司 | Plant growth kinetics captured by motion tracking |
| CN103091296A (en) * | 2013-01-23 | 2013-05-08 | 浙江大学 | Plant physical condition detection method based on spectral imaging technology, and device for same |
| CN204026228U (en) * | 2014-07-09 | 2014-12-17 | 宁波米德兰电子制造有限公司 | LED plant growth lamp |
| CN104807761A (en) * | 2015-05-08 | 2015-07-29 | 南开大学 | Design method of spectrograph for realizing micro-area spectral measurement |
| CN105651785A (en) * | 2015-12-31 | 2016-06-08 | 中国科学院半导体研究所 | Microscopic imaging device and method for measuring microstructure defects on surface of semiconductor material |
| CN106922414A (en) * | 2017-02-27 | 2017-07-07 | 广东工业大学 | A kind of intelligent controlling device and method that plant growth lighting is carried out with LED |
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| Title |
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Application publication date: 20180420 |