CN107937536A - 胶质瘤预后标志物circ19:47362476|47362693及应用 - Google Patents
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Abstract
本发明公开了一种胶质瘤预后标志物circ 19:47362476|47362693及应用，即检测胶质瘤组织中circRNA circ 19:47362476|47362693的试剂用于制备胶质瘤患者的预后制剂。通过研究证实胶质瘤中circRNA circ 19:47362476|47362693表达量较高的患者，术后生存率更高。通过检测胶质瘤患者胶质瘤组织中circRNA circ 19:47362476|47362693的表达水平，从而对胶质瘤患者做出预后判断。
Description
技术领域
本发明属于生物技术领域，涉及一种用于胶质瘤预后的circRNA标志物、以及检测该标志物的试剂用于制备胶质瘤预后制剂的应用、还有试剂盒。
背景技术
脑胶质瘤是成年人最为频发的脑肿瘤疾病，占颅内肿瘤的40.49％。从确诊开始，脑胶质瘤患者平均生存寿命不超过五年。为了诊治胶质瘤这种与遗传物质相关性极高的恶性疾病，必须在分子生物学水平，从遗传信息表达的方面来探索其发病机理。目前而言胶质瘤的诊断和治疗方法虽处于不断改进阶段，但是胶质瘤患者生存率并没有明显提高。胶质瘤诊断仍然处于以临床、病理学和影像学信息为基础的经验性阶段，而且一经诊断，绝大多数均为中晚期，手术后的生存率不容乐观。因此，寻找胶质瘤预后标志物对患者进行预后分析，以提高胶质瘤患者的术后生活质量，并相应地选择合理的后续治疗方案，提高生存率，是神经科学领域亟待解决研究任务。
circRNA是一类广泛且多样地存在于哺乳动物细胞中、具有调控基因表达作用的内源性非编码RNA分子，具有共价闭合的环形结构，广泛存在于各种细胞中，也是继microRNA(miRNA)后RNA家族的最新研究热点。近年来，随着深度测序技术的广泛应用和生物物理和信息学技术的快速发展，人们发现人类许多外显子的转录本可被非线性地反向剪接或通过基因重排而形成circRNA，且它们在所有剪接转录本中占了相当大的比例，并具有丰富性、稳定性、高保守性和时空特异性等特征，有作为诸多疾病分子标志物的潜力。
发明内容
本发明的第一个目的是：提供一种用于胶质瘤患者预后的胶质瘤组织来源的circRNA标志物circ 19:47362476|47362693，其序列如SEQ NO:1所示。
本发明的第二个目的是，提供检测所述的circRNA标志物在胶质瘤组织中表达量的试剂在制备胶质瘤预后制剂中的应用。
本发明的第三个目的是，提供一种胶质瘤预后试剂盒，能够测定胶质瘤组织中的circ 19:47362476|47362693的含量。
所述的胶质瘤预后试剂盒，含有检测circ 19:47362476|47362693含量的PCR引物。优选引物的序列如SEQ NO:2和3所示。
所述的胶质瘤预后试剂盒，除circ 19:47362476|47362693的引物外，还含有从胶质瘤组织中提取RNA并进行逆转录及荧光定量PCR的所有试剂。包括：
(1)从胶质瘤组织中抽提总RNA所用试剂，包括RNA稳定溶液、Trizol试剂、三氯甲烷、异丙醇、无酶水；
(2)以总RNA为模板将circ 19:47362476|47362693逆转录为cDNA所用试剂，包括逆转录缓冲液、三磷酸碱基脱氧核苷酸、RNA酶抑制剂、MMLV逆转录酶以及circ 19:47362476|47362693所用随机引物；
(3)将cDNA实时定量PCR所用试剂，包括circRNA circ 19:47362476|47362693实时荧光定量PCR特异性引物、GAPDH内参特异性PCR引物、实时荧光定量SYBR染料、无酶水。
申请人通过荧光定量PCR与生存曲线分析发现胶质瘤组织来源的circRNA circ19:47362476|47362693与患者的生存率相关，含量越高，生存率越高。此法为胶质瘤的预后分析提供了强有力的技术支持，有助于提高胶质瘤患者的术后生活质量，制订术后治疗方案，提高生存率，具有深远的临床意义和推广性。
附图说明
图1为实时荧光定量PCR分析circ 19:47362476|47362693在正常脑组织(12例)与胶质瘤 (42例)中的表达差异；
病例组胶质瘤组织中circ 19:47362476|47362693表达水平较对照组显著降低(p＝0.0002)；
图2为生存曲线分析胶质瘤组织来源的circ 19:47362476|47362693表达高低对胶质瘤患者的预后影响。
生存曲线分析显示，胶质瘤组织来源的circRNA circ 19:47362476|47362693与患者的生存率相关(P＝0.047)，含量越高，生存率越高。
具体实施方式
以下结合实施例旨在进一步说明本发明，而非限制本发明。
实施例1制备检测circRNA circ 19:47362476|47362693表达量的试剂用于制备胶质瘤患者预后的试剂盒(50次反应)
1.RNA稳定溶液50ml
2.异丙醇100ml
3.三氯甲烷100ml
4.Trizol 50ml
5.无酶水10ml
6.1μM随机逆转录引物50μl
7.5×逆转录缓冲液200ml
8.10mM三磷酸碱基脱氧核苷酸100μl
9.40U/μl RNA 酶抑制剂500μl
10.200U/μl MMLV逆转录酶50μl
11.Premix Ex Taq 50μl
12.10μM circRNA circ 19:47362476|47362693实时荧光定量PCR特异性引物30μl
circRNA circ 19:47362476|47362693正向引物：5'-TCTGCTTCCGCCTCTATGC-3'，
circRNA circ 19:47362476|47362693反向引物5'-CTGCGCTGGCCTGACTAA-3'；
13.10μM GAPDH特异性引物30μl
正向引物为5′-ATCATCAGCAATGCCTCCT-3′，
反向引物为5′-CATCACGCCACAGTTTCC-3′。
实施例2 circRNA circ 19:47362476|47362693在胶质瘤组织的表达量与预后的关系
1、收集待测胶质瘤组织放入盛有RNA稳定溶液的冻存管中，放至-80℃冰箱备用。
2、组织中RNA 的抽提：取适量标本于经180℃烘烤6-8h后的研钵中加入液氮研磨标本，研磨至粉末状后于研钵中加入1ml Trizol研钵标本，研磨成液体状后移至tube管，于冰上静止裂解15分钟。裂解结束后4℃，12000rpm离心10min，上清液移至新的tube管。加氯仿200μl于Tube中，用手震荡15-30s，冰上放置5min，4℃，12000rpm离心15min；小心取上层水相入新tube中，加入预冷的异丙醇0.5ml混匀，冰上静置20min，4℃， 12000rpm离心10min；弃上清，加入75％DEPC水稀释的乙醇1-2ml混匀，4℃， 7500rpm离心5min，尽量弃上清，室温干燥5-10min，加入DEPC水20μl溶解RNA，-80℃保存。
3、circRNA circ 19:47362476|47362693逆转录：使用Thermo公司的逆转录试剂盒。20μl 逆转录反应的体系如下：
| 成分 | 剂量/管 |
| 随机逆转录引物(1μM) | 1μl |
| RNA样本 | 2μg |
| 无酶水 | To 12μl |
逆转录第一步条件：65℃5分钟
| 成分 | 剂量/管 |
| 5×逆转录缓冲液 | 4μl |
| 三磷酸碱基脱氧核苷酸(10mM) | 2μl |
| RNA酶抑制剂(40U/μl) | 1μl |
| MMLV逆转录酶(200U/μl) | 1μl |
| 第一步逆转录的产物 | 12μl |
| 总体积 | 20μl |
逆转录第二步程序：25℃5分钟，42℃60分钟，70℃5分钟。
4、汉恒生物科技(上海)有限公司合成的circ 19:47362476|47362693特异性引物进行实时定量PCR：先将逆转录产物稀释10倍，混匀。20μl反应体系如下：
PCR的特异性引物：
正向引物：5'-TCTGCTTCCGCCTCTATGC-3'，
反向引物：5'-CTGCGCTGGCCTGACTAA-3'。
GAPDH内参特异性PCR引物：
正向引物为5′-ATCATCAGCAATGCCTCCT-3′，
反向引物为5′-CATCACGCCACAGTTTCC-3′。
实时荧光定量PCR反应程序：95℃3分钟,40个循环，95℃10秒，60℃30秒。
5、2-ΔΔCT指标的测定：本实验数据采用相对定量的分析方法，△CT＝CT样本–CT内参，△△CT＝△CT–△CT正常，GAPDH作为内参基因，ΔCT正常为正常血清外泌体的ΔCT平均值。数据利用软件GraphPad Prism和SPSS17.0进行分析。一共分析了54例组织样本，分析发现，与胶质瘤组织中circRNA circ 19:47362476|47362693低表达(低于平均值)的患者相比，胶质瘤组织中circRNA circ 19:47362476|47362693高表达(高于平均值)的患者存活率更高，差异有显著性(P＝0.047)，见图2。
以上研究表明，circ 19:47362476|47362693可作为胶质瘤患者预后的特异性分子标志物。
序列表
<110> 中南大学湘雅医院
<120> 胶质瘤预后标志物circ 19:47362476|47362693及应用
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 218
<212> RNA
<213> 智人(Homo sapiens)
<400> 1
gaaaggugaa ggagaugagc uacgauccgc aggccaagcu gcaacggcug caggaguucu 60
ggauuaguca ggccagcgca gagcagcgga agggccgggc gggccgcacg ggccccggag 120
ucugcuuccg ccucuaugcc gaaucggacu augaugccuu cgcccccuac cccgucccag 180
aaauucggag gguggcccug gacucguugg ugcugcag 218
<210> 2
<211> 19
<212> DNA
<213> 未知(Unknown)
<400> 2
tctgcttccg cctctatgc 19
<210> 3
<211> 18
<212> DNA
<213> 未知(Unknown)
<400> 3
ctgcgctggc ctgactaa 18
<210> 4
<211> 19
<212> DNA
<213> 未知(Unknown)
<400> 4
atcatcagca atgcctcct 19
<210> 5
<211> 18
<212> DNA
<213> 未知(Unknown)
<400> 5
catcacgcca cagtttcc 18
Claims (6)
1.一种胶质瘤预后标志物circ 19:47362476|47362693，其序列如SEQ NO:1所示。
2.检测权利要求1所述的标志物在胶质瘤组织中表达量的试剂在制备胶质瘤预后制剂中的应用。
3.一种胶质瘤预后试剂盒，其特征在于，能够测定胶质瘤组织中的circ 19:47362476|47362693的含量。
4.根据权利要求3所述的胶质瘤预后试剂盒，其特征在于，含有检测circ 19:47362476|47362693含量的PCR引物。
5.根据权利要求4所述的胶质瘤预后试剂盒，其特征在于，引物的序列如SEQ NO:2和3所示。
6.根据权利要求3或4或5所述的胶质瘤预后试剂盒，其特征在于，除circ 19:47362476|47362693的引物外，还含有从胶质瘤组织中提取RNA并进行逆转录及荧光定量PCR的所有试剂。
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| CN107937536A true CN107937536A (zh) | 2018-04-20 |
| CN107937536B CN107937536B (zh) | 2020-07-17 |
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Non-Patent Citations (3)
| Title |
|---|
| RYBAK-WOLF ET AL.: "Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed.", 《MOLECULAR CELL》 * |
| SALZMAN ET AL.: "Cell-Type Specific Features of Circular RNA Expression.", 《PLOS GENETICS》 * |
| XIAOFENG SONG ET AL.: "Circular RNA profile in gliomas revealed by identification tool UROBORUS.", 《NUCLEIC ACIDS RESEARCH》 * |
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