CN107937260A - A kind of method that immobilization fermentation reduces monascorubin citrinin - Google Patents

A kind of method that immobilization fermentation reduces monascorubin citrinin Download PDF

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CN107937260A
CN107937260A CN201711319707.9A CN201711319707A CN107937260A CN 107937260 A CN107937260 A CN 107937260A CN 201711319707 A CN201711319707 A CN 201711319707A CN 107937260 A CN107937260 A CN 107937260A
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fermentation
tray
pipe
air intlet
immobilization
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CN107937260B (en
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薛栋升
周方谱
曾徐浩
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Hangzhou Tianqu Biotechnology Co ltd
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Hubei University of Technology
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Abstract

The invention discloses a kind of method that immobilization fermentation reduces monascorubin citrinin, belong to field of fermentation engineering.The method of the present invention includes the following steps：Monascus bacterium solution is added in the polyvinyl alcohol containing sodium alginate, is added containing CaCl2Boric acid fixer, soak more than 4h, monascus immobilization particle is obtained by filtration；Monascus immobilization particle is added in the tray of Zymolysis Equipment tray tank, is passed through culture medium, by controlling the air mass flow of air intlet pipe A, B, C to promote the dissolving of oxygen in culture medium, fermented to obtain fermentation liquid according to certain technique；Repeat to ferment multiple batches of, citrinin content is reduced in different batches fermentation liquid.The present invention reduces the content of monascorubin citrinin in immobilization fermentation technique by designing new Zymolysis Equipment.

Description

A kind of method that immobilization fermentation reduces monascorubin citrinin
Technical field
The present invention relates to field of fermentation engineering, and in particular to a kind of immobilization fermentation reduces the side of monascorubin citrinin Method.
Background technology
Monascorubin is the natural pigment that monascus produces during growth metabolism, due to its with color and luster it is scarlet, The advantages that color power is strong, stability is good, taste is natural, the coloring of food is widely used in China.Monascorubin also has a variety of lifes Thing activity, such as inducing carcinogenic effect to some conditions has obvious inhibitory action.Nineteen ninety-five France scholar BLANC P J etc. is confirmed Monascus (Monascus purpureus) CBS 109.07 and (Monascus ruber van Tieghem) are in metabolism of pigment While also produce Monascidin A, and confirm that the material is mycotoxin-citrinin, so as to cause people to red yeast rice color The concern of plain security.Citrinin is also known as citrinin, initially finds to be produced by Penicillium notatum the 1930s, is taken as anti- Raw element is paid much attention to, after because finding that it, with renal toxicity, eliminates the possibility as treatment antibiotic, and regard as true Verticillium toxin.The target organ of citrinin is kidney, the Toxicity of Kidney that can cause various experimental animals into citrinin is taken, it is characterized in that kidney It is dirty to become larger, ultimately result in kidney failure.Also studies have found that, citrinin has teratogenesis, damages the metabolism of liver, has carcinogenicity and lures Mutation effect is sent out, and may the generation suppression of Central nervous system.
The production of China red yeast rice and applicating history are long, scale constantly expands, but due to citrinin in monascus product not It can be effectively controlled, cause monascus product there are potential safety issue, and the export abroad trade to China's red yeast rice is made Into great limitation.Therefore, seeking to reduce the method for citrinin content in monascus product becomes the key to solve the above problems.
The content of the invention
The object of the present invention is to overcome the problems of the prior art, there is provided a kind of immobilization fermentation reduces monascorubin The method and Zymolysis Equipment of citrinin.
The purpose of the present invention is achieved through the following technical solutions：
A kind of Zymolysis Equipment tray tank, including tank body.The tank interior side is installed with multiple trays, the tank Internal portion's opposite side is not contacted with tray.The tray is internally provided with strainer close to the edge of tank body opposite side.The tank body bottom Portion is equipped with steam inlet pipe, top is equipped with steam outlet pipe and is used to sterilize, and the steam inlet pipe, steam outlet pipe are equipped with list To valve.The tank base side is connected with outlet valve, turbine pump two, discharge port in turn by hose one, and the discharge port is set There is check valve.The end connection mixing jetting structure of the hose one, the mixing jetting structure are mixed by being connected by pipeline Close injection structure A, B, C composition.The mixing jetting structure A includes the big expanding chamber A of spray chamber, the small expanding chamber A of spray chamber；It is described The big expanding chamber A of spray chamber is connected with air intlet pipe A and is used to be passed through filtrated air；The big expanding chamber A of spray chamber is also associated with Liquid inlet tube is used to be passed through culture medium or other liquid (such as acid ＆ alkali liquid, aqua sterilisa), liquid inlet tube be equipped with turbine pump one, Inlet valve.The mixing jetting structure B includes the big expanding chamber B of spray chamber, the small expanding chamber B of spray chamber, the big expansion of spray chamber Air intlet pipe B is connected with the B of room to be used to be passed through filtrated air.The mixing jetting structure C includes the big expanding chamber C of spray chamber, Air intlet pipe C is connected with the big expanding chamber C of spray chamber to be used to be passed through filtrated air.The air intlet pipe A, air intlet pipe B, air intlet pipe C is equipped with air filter, air flow meter and air flow rate adjustment valve.The mixing jetting structure C The port of export is each passed through tank body by many flexible pipes two and is connected with tray, and the hose two is equipped with check valve.On the tank body also Pipe is added through the solid material that tank body is connected with tray equipped with more, the solid material adds pipe and is equipped with diaphragm valve, adding Diaphragm valve is closed after entering solid material.
Preferably, the tray in the vertical direction is equidistantly installed.
Preferably, pipe sleeve is cased with the hose one, the pipe sleeve has equipped with hot water inlet, hot water outlet, by being passed through The water of certain temperature is to maintain the temperature of nutrient solution in hose one.
Preferably, the hose one, hose two are stainless steel flexible hose, and diameter is respectively 7cm, 2cm；The solid material adds Enter the stainless steel tube that pipe is diameter 3cm.The tank body, tray are process with the A3 type stainless steels of 3cm thickness.
The tank interior is normal line apart from tank bottom 7-10cm.
The marker method of the Zymolysis Equipment is：After fermentation, turbine pump two is opened, discharge port is opened, when tank body Bottom liquid empties, and closes turbine pump two, closes discharge port.The angled zymotic fluid made in tray of tank body is tilted to flow out, Turbine pump two is again turned on, discharge port is opened, until tank liquid is all given off.
A kind of method that immobilization fermentation reduces monascorubin citrinin, includes the following steps：
(1) preparation of monascus immobilization particle
Monascus bacterium solution is added in the polyvinyl alcohol containing sodium alginate, is added containing CaCl2Boric acid fixer, immersion More than 4h, is obtained by filtration monascus immobilization particle.
(2) fermented using above-mentioned Zymolysis Equipment
First batch is fermented：Pipe is added by solid material monascus immobilization particle is added in tray, open turbine Pump one, open inlet valve, add fermentation medium into each tray, when after the culture medium of tray tank reaches normal line when Wait, open outlet valve, open turbine pump two, allow all pipelines are interior to be full of fermentation medium, close inlet valve.Turbine pump two is always Open, allow the flow velocity of fermentation medium be 30-35mL/min, while open the valve of air intlet pipe A, B, C, adjusting air stream Amount, is adjusted to 30-36mL/min, air intlet pipe B throughputs are adjusted to 7-8mL/min, air intlet by air intlet pipe A throughputs Pipe C throughputs are adjusted to 2-3mL/min, and ferment 5-6d；Air intlet pipe A throughputs are adjusted to 12-15mL/min, air intlet pipe B throughputs are adjusted to 6-8mL/min, air intlet pipe C throughputs are adjusted to 1-2mL/min, and ferment 10-15d；By air intlet pipe A Throughput is adjusted to 8-10mL/min, air intlet pipe B throughputs are adjusted to 4-6mL/min, air intlet pipe C throughputs are adjusted to 0.3- 0.5mL/min, ferment 2-3d, obtains fermentation liquid, opens discharge port, releases first batch fermentation liquid, monascus immobilization Particle is intercepted the fermentation for being used for next batch in tray by strainer.During fermentation, temperature control is 25-30 DEG C.
Operation according to first batch fermentation carries out second lot, the 3rd batch, the 4th Batch fermentation.
Step (1) is preferably：300-350mL monascuses bacterium solution and 900-1000mL is poly- containing 0.6% sodium alginate 600 solution of vinyl alcohol is uniformly mixed, and is instilled by syringe and is contained 10%CaCl25% boric acid fixer in, particle diameter 3- is made The particle of 4mm, soaks more than 4h, filters out immobilization particle, and monascus immobilization particle is obtained afterwards three times with physiology salt washing.
The formula of fermentation medium described in step (2) is preferably：Rice meal 5%, wheat bran 0.1%, KNO3 0.15%, NH4NO30.1%, KH2PO40.3%, K2HPO40.3%, MgSO4·7H2O 0.8%, KCl 0.05%, FeSO4·7H2O0.001%, ZnSO4·7H2O 0.001%, pH 5.5.
The present invention reduces containing for monascorubin citrinin in immobilization fermentation technique by designing new Zymolysis Equipment Amount.
Brief description of the drawings
Fig. 1 is the structure diagram of Zymolysis Equipment of the present invention；
Fig. 2 is the top view of tray in Zymolysis Equipment of the present invention；
In figure, 1- tank bodies, 2- trays, 3- steam inlet pipes, 4- steam outlet pipes, 5- strainers, 6- solid materials, which add, manages, 7- outlet valves, 8- turbine pumps two, 9- discharge ports, 10- hoses one, the big expanding chamber A of 11- spray chambers, the big expanding chamber B of 12- spray chambers, The big expanding chamber C of 13- spray chambers, 14- liquid inlet tubes, 15- turbine pumps one, 16- inlet valves, 17- air intlet pipes A, 18- injection The small expanding chamber A of chamber, 19- spray chambers small expanding chamber B, 20- air intlet pipe B, 21- air intlet pipe C, 22- hose two, 23- pipes Set, 24- hot water inlets, 25- hot water outlets, 26- normal lines.
Embodiment
Following embodiments are used to further illustrate the present invention, but should not be construed as limiting the invention.If do not refer in particular to Conventional means bright, that technological means used is well known to those skilled in the art in embodiment.
Embodiment 1
A kind of Zymolysis Equipment tray tank, structure as shown in Figure 1, 2, including tank body 1.1 interior side of tank body fixes peace Equipped with multiple trays 2, the 1 inside opposite side of tank body is not contacted with tray 2.2 in the vertical direction of tray is equidistantly pacified Dress；The tray 2 is internally provided with strainer 5 close to the edge of 1 opposite side of tank body.1 bottom of tank body be equipped with steam inlet pipe 3, Top is equipped with steam outlet pipe 4 and is used to sterilize, and the steam inlet pipe 3, steam outlet pipe 4 are equipped with check valve.The tank body 1 Bottom side is connected with outlet valve 7, turbine pump 28, discharge port 9 in turn by hose 1, and the discharge port 9 is equipped with unidirectional Valve.Pipe sleeve 23 is cased with the hose 1, the pipe sleeve 23 has equipped with hot water inlet 24, hot water outlet 25, by being passed through one The water of constant temperature degree is to maintain the temperature of nutrient solution in hose 1.The end connection mixing jetting structure of the hose 1, institute Mixing jetting structure is stated to be made of mixing jetting structure A, B, the C connected by pipeline.The mixing jetting structure A includes injection The big expanding chamber A11 of chamber, the small expanding chamber A18 of spray chamber；The big expanding chamber A11 of spray chamber is connected with air intlet pipe A17 and is used for It is passed through filtrated air；The big expanding chamber A11 of spray chamber is also associated with liquid inlet tube 14 and is used to be passed through culture medium or other liquid Body (such as acid ＆ alkali liquid, aqua sterilisa), liquid inlet tube 14 are equipped with turbine pump 1, inlet valve 16.The mixing jetting structure B bags Include the big expanding chamber B12 of spray chamber, be connected with air intlet pipe on spray chamber small expanding chamber B19, the big expanding chamber B12 of spray chamber B20 is used to be passed through filtrated air.The mixing jetting structure C includes the big expanding chamber C13 of spray chamber, the big expanding chamber C13 of spray chamber On be connected with air intlet pipe C21 be used for be passed through filtrated air.The air intlet pipe A17, air intlet pipe B20, air into Mouth pipe C21 is equipped with air filter, air flow meter and air flow rate adjustment valve.The port of export of the mixing jetting structure C Tank body 1 is each passed through by many flexible pipes 2 22 to connect with tray 2, the hose 2 22 is equipped with check valve.On the tank body 1 also Pipe 6 is added through the solid material that tank body 1 is connected with tray 2 equipped with more, the solid material adds pipe 6 and is equipped with diaphragm valve, Diaphragm valve is closed after solid material is added.
Specifically, the hose 1, hose 2 22 are stainless steel flexible hose, diameter is respectively 7cm, 2cm；The solids Material adds the stainless steel tube that pipe 6 is diameter 3cm.The tank body 1, tray 2 are process with the A3 type stainless steels of 3cm thickness.It is described Tank interior is normal line 26 apart from tank bottom 7-10cm.
The marker method of the Zymolysis Equipment is：After fermentation, turbine pump 28 is opened, discharge port 9 is opened, when tank Body bottom liquid empties, and closes turbine pump 28, closes discharge port 9.Tilt the angled fermentation broth stream made in tray of tank body Go out, be again turned on turbine pump 28, discharge port 9 is opened, until tank liquid is all given off.
Embodiment 2
1st, material
Monascus：Monascus CCTCCAF93208.PVAC polyvinylalcohol -124：Japanese import packing.Sodium alginate： MerckCO.Inc。
2nd, the preparation of culture medium
Wort agar inclined-plane culture medium (concentration is mass fraction)：Glucose 1%, yeast extract 0.3%, brewer's wort powder 0.2%, peptone 0.3%, agar 2%, pH6.0,121 DEG C sterilizing 30min.
Seed culture medium (concentration is mass fraction)：Glucose 1%, yeast extract 0.3%, brewer's wort powder 0.2%, albumen Peptone 0.3%, pH6.0.The bottled 75mL seed culture mediums of 500mL triangles, 121 DEG C of sterilizing 30min.
Fermentation Condition of Monascus spp culture medium (concentration is mass fraction)：Rice meal (after rice is crushed with pulverizer, crosses 40 mesh Sieve) 5%, wheat bran (40 mesh sieves are crossed after crushing) 0.1%, KNO30.15%, NH4NO30.1%, KH2PO40.3%, K2HPO40.3%, MgSO4·7H2O0.8%, KCl0.05%, FeSO4·7H2O0.001%, ZnSO4·7H2O0.001%, PH5.5,121 DEG C of sterilizing 30min.
3rd, method
(1) after monascus CCTCC AF93208 are inoculated on wort agar inclined-plane culture medium 7- is cultivated in 30 DEG C of dark 8d, obtains monascus slant pore.
Lower inclined plane spore is washed with Sterile Salines of the 10mL containing 0.9%NaCl, then toward equipped with 75mL seed culture mediums 2mL spore suspensions are accessed in 500mL triangular flasks, and (concentration microscopy is 2 × 105A/mL), 180r/min is in rotary shaker at 30 DEG C Upper lucifuge culture 36h, obtains monascus seed culture fluid.
(2) preparation of monascus immobilization particle
300mL monascuses seed culture fluid and 900mL are aseptically contained into 0.6% (mass fraction, similarly hereinafter) seaweed 600 solution of polyvinyl alcohol of sour sodium is uniformly mixed, and is instilled by syringe and is contained 10%CaCl25% boric acid fixer in, be made The particle of particle diameter 3-4mm, soaks more than 4h, filters out immobilization particle, and monascus immobilization is obtained afterwards three times with physiology salt washing Particle.The amount for obtaining monascus immobilization particle is the monascus immobilization particle of a tray amount.
(3) immobilization fermentation
1) equipment sterilizes
Vapor is passed through in Zymolysis Equipment tray tank into embodiment 1, sterilize 30min at 121 DEG C, cooling down to room Temperature.The tray tank a diameter of 1.0m, the high 2.0m of jar；There are 12 trays, a diameter of 0.8m of tray, tray is highly 0.15m, the capacity of tray is 23L.
2) monascus immobilization particle loads tray tank
The monascus immobilization particle of one tray amount is added pipe from solid material to be added separately in each tray.
3) ferment
First batch is fermented：Into pipe sleeve, circulation is passed through 30 DEG C of warm water, opens turbine pump one, opens inlet valve, to every Fermentation medium is added in a tray, (tray pot bottom reaches the distance of normal line when the culture medium of tray tank reaches normal line It is 7cm) when, outlet valve is opened, opens turbine pump two, allows all pipelines are interior to be full of fermentation medium, closes inlet valve, add hair The total amount of ferment culture medium is 340L.Turbine pump two is always on, allows the flow velocity of fermentation medium to be 30mL/min, while open sky The valve of gas inlet tube A, B, C, adjust air mass flow, air intlet pipe A throughputs are adjusted to 30mL/min, air intlet pipe B Throughput is adjusted to 7mL/min, air intlet pipe C throughputs are adjusted to the 3mL/min (differences of the sub- air velocitys of air intlet pipe A, B, C Different is to increase the turbulent extent of liquid to greatest extent, promotes the dissolving of oxygen), ferment 5d；By air intlet pipe A throughputs It is adjusted to 15mL/min, air intlet pipe B throughputs are adjusted to 6mL/min, air intlet pipe C throughputs are adjusted to 1mL/min, fermentation 15d；Air intlet pipe A throughputs are adjusted to 8mL/min, air intlet pipe B throughputs are adjusted to 4mL/min, air intlet pipe C leads to Tolerance is adjusted to 0.5mL/min, and ferment 2d, obtains the fermentation liquid of maturation, opens discharge port, releases first batch fermentation liquid. Release fermentation liquid operation be specially：Turbine pump two is opened, opens discharge port, when tank base emptying rate of liquid, closes whirlpool Wheel pump two, closes discharge port, inclination tank body is angled, flows out the fermentation liquid in tray, and monascus immobilization Grain is intercepted the fermentation for being used for next batch in tray by strainer, is again turned on turbine pump two, discharge port is opened, until tank liquid All given off.During fermentation, controlled at 28-30 DEG C.
Operation according to first batch fermentation carries out second lot, the 3rd batch, the 4th Batch fermentation, obtains second batch Secondary, the 3rd batch, the fermentation liquid of the 4th batch.
(4) Fermentation Condition of Monascus spp mash color value and citrinin content measure
1) measure of pigment color value
Alcohol-soluble pigment color value assay method：Monascus fermentation broth centrifuges 10min in 5000r/min, takes supernatant, uses After 70% ethanol dilution certain multiple, 60 DEG C of water-bath 1h, filtering；After filtrate dilutes certain multiple with 70% ethanol again, survey respectively Determine OD505、OD465、OD410, according to color value=OD × extension rate/acquired fermentating liquid volume, be calculated alcohol-soluble it is red, Orange, xanthein color value.
Water colo(u)r color value assay method：Monascus fermentation broth centrifuges 10min in 5000r/min, supernatant is taken, with steaming After distilled water dilution certain multiple, 60 DEG C of water-bath 1h, filtering；After filtrate dilutes certain multiple with distilled water again, OD is used respectively505、 OD465、OD410, according to color value=OD × extension rate/acquired fermentating liquid volume, it is calculated water-soluble red, orange, yellow The color value of color pigment.
Total pigment concentration for alcohol-soluble pigment color value and water colo(u)r color value and.
2) measure of citrinin content
Take Monascus fermentation broth 10mL to centrifuge 10min in 5000r/min, it is molten that 70% ethanol of 20mL is added into supernatant Liquid mixes, and high performance liquid chromatography inspection is carried out according to standard GB/T/T 5009.222-2008 with after 0.22 μm of filtering with microporous membrane Survey.
After measured, first batch, second lot, the 3rd batch, the pigment concentration of the 4th Batch fermentation mash are 1.38U/ mL、1.45U/mL、1.28U/mL、1.20U/mL；Tangerine in first batch, second lot, the 3rd batch, the 4th Batch fermentation mash Mycin content is 0.0176mg/mL, 0.0178mg/mL, 0.0179mg/mL, 0.0196mg/mL.
Contrast test 1
262L fermentations are added in 400L air blow tank with machinery agitation (Zhenjiang east bioengineering equipment Co., Ltd) Culture medium, accesses the monascus immobilization particle of 12 disk amounts, adjusts air mass flow, and air vent amount is 40mL/min, hair Ferment 5d；It is 22mL/min to adjust air vent amount, and ferment 15d；It is 12.5mL/min to adjust air vent amount, and ferment 2d, opens Discharge port, the mash of discharge are first fermentation liquid with 20 mesh screens, filtrate, and monascus immobilization particle is retained by sieve Ferment for next batch.
Operation according to first batch fermentation carries out second lot, the 3rd batch, the 4th Batch fermentation, obtains second batch Secondary, the 3rd batch, the fermentation liquid of the 4th batch.After measured, first batch, second lot, the 3rd batch, the 4th Batch fermentation The pigment concentration of mash is 1.36U/mL, 1.40U/mL, 1.20U/mL, 1.19U/mL；First batch, second lot, the 3rd batch In secondary, the 4th Batch fermentation mash citrinin content for 0.0216mg/mL, 0.0221mg/mL, 0.0226mg/mL, 0.0230mg/mL。
Contrast test 2
In addition to air mass flow is adjusted, other operations obtain the fermentation liquid of four batches with embodiment 2.Air stream measurer Body is adjusted to：Air intlet pipe A throughputs are 33mL/min, and air intlet pipe B throughputs are 7mL/min, and air intlet pipe C leads to Tolerance is 0mL/min, and ferment 5d；It is 16mL/min by air intlet pipe A throughputs, air intlet pipe B throughputs are 6mL/ Min, air intlet pipe C throughput are 0mL/min, and ferment 15d；Be 8.5mL/min by air intlet pipe A throughputs, air into Mouth pipe B throughputs are 4mL/min, and air intlet pipe C throughputs are 0mL/min, and ferment 2d, obtains the fermentation liquid of maturation.
After measured, first batch, second lot, the 3rd batch, the pigment concentration of the 4th Batch fermentation mash are 1.31U/ mL、1.34U/mL、1.18U/mL、1.10U/mL；Tangerine in first batch, second lot, the 3rd batch, the 4th Batch fermentation mash Mycin content is 0.0183mg/mL, 0.0181mg/mL, 0.0192mg/mL, 0.0199mg/mL.
Contrast test 3
Remove the tray in tray tank, add the monascus immobilization particle identical with 2 dosage of embodiment and fermented and cultured Base, ferments according to the throughput set in embodiment 2, obtains the fermentation liquid of four batches.
After measured, first batch, second lot, the 3rd batch, the pigment concentration of the 4th Batch fermentation mash are 1.21U/ mL、1.26U/mL、1.11U/mL、1.05U/mL；Tangerine in first batch, second lot, the 3rd batch, the 4th Batch fermentation mash Mycin content is 0.0193mg/mL, 0.0191mg/mL, 0.0199mg/mL, 0.0205mg/mL.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (8)

1. a kind of Zymolysis Equipment tray tank, including tank body, it is characterised in that：The tank interior side is installed with multiple shallow Disk, the tank interior opposite side are not contacted with tray；The tray is internally provided with strainer close to the edge of tank body opposite side；Institute State tank base and be equipped with steam (vapor) outlet equipped with steam inlet pipe, top；The tank base side is sequentially connected by hose one There are outlet valve, turbine pump two, discharge port；The hose one end connection mixing jetting structure, the mixing jetting structure by Mixing jetting structure A, B, the C connected by pipeline is formed；The mixing jetting structure A includes the big expanding chamber A of spray chamber, injection The small expanding chamber A of chamber；The big expanding chamber A of spray chamber is connected with air intlet pipe A, and the big expanding chamber A of spray chamber is also associated with Liquid inlet tube, liquid inlet tube are equipped with turbine pump one, inlet valve；The mixing jetting structure B includes the big expansion of spray chamber Air intlet pipe B is connected with room B, spray chamber small expanding chamber B, the big expanding chamber B of spray chamber；The mixing jetting structure C Including the big expanding chamber C of spray chamber, air intlet pipe C is connected with the big expanding chamber C of spray chamber；The air intlet pipe A, air into Mouth pipe B, air intlet pipe C are equipped with air filter, air flow meter and air flow rate adjustment valve；The mixing jetting structure The port of export of C is each passed through tank body by many flexible pipes two and is connected with tray；Be additionally provided with the tank body more through tank body with The solid material of tray connection adds pipe.
2. Zymolysis Equipment tray tank according to claim 1, it is characterised in that：The tray in the vertical direction is equidistant Installation.
3. Zymolysis Equipment tray tank according to claim 1, it is characterised in that：Pipe sleeve is cased with the hose one, it is described Pipe sleeve has equipped with hot water inlet, hot water outlet.
4. Zymolysis Equipment tray tank according to claim 1, it is characterised in that：The tank interior is apart from tank bottom 7-10cm For normal line.
5. a kind of method that immobilization fermentation reduces monascorubin citrinin, it is characterised in that：Include the following steps：
(1) preparation of monascus immobilization particle
Monascus bacterium solution is added in the polyvinyl alcohol containing sodium alginate, is added containing CaCl2Boric acid fixer, immersion 4h with On, monascus immobilization particle is obtained by filtration；
(2) usage right requires 1-4 any one of them Zymolysis Equipment to ferment
First batch is fermented：Pipe is added by solid material monascus immobilization particle is added in tray, open turbine pump one, Inlet valve is opened, fermentation medium is added into each tray, when after the culture medium of tray tank reaches normal line, is opened Outlet valve, opens turbine pump two, allows all pipelines are interior to be full of fermentation medium, closes inlet valve；Turbine pump two is always on, allows The flow velocity of fermentation medium is 30-35mL/min, while opens the valve of air intlet pipe A, B, C, air mass flow is adjusted, by sky Gas inlet tube A throughputs are adjusted to 30-36mL/min, air intlet pipe B throughputs are adjusted to 7-8mL/min, air intlet pipe C ventilation Amount is adjusted to 2-3mL/min, and ferment 5-6d；Air intlet pipe A throughputs are adjusted to 12-15mL/min, air intlet pipe B throughputs It is adjusted to 6-8mL/min, air intlet pipe C throughputs are adjusted to 1-2mL/min, fermentation 10-15d；By air intlet pipe A throughput tune 4-6mL/min is adjusted to for 8-10mL/min, air intlet pipe B throughputs, air intlet pipe C throughputs are adjusted to 0.3-0.5mL/ Min, ferment 2-3d, obtains fermentation liquid, opens discharge port, releases first batch fermentation liquid, monascus immobilization particle quilt Strainer intercepts the fermentation for being used for next batch in tray；
Operation according to first batch fermentation carries out second lot, the 3rd batch, the 4th Batch fermentation.
6. the method that immobilization fermentation according to claim 5 reduces monascorubin citrinin, it is characterised in that：Step (1) be：300-350mL monascuses bacterium solution is mixed with 600 solution of polyvinyl alcohol of the 900-1000mL containing 0.6% sodium alginate Uniformly, instilled by syringe and contain 10%CaCl25% boric acid fixer in, be made the particle of particle diameter 3-4mm, immersion 4h with On, immobilization particle is filtered out, monascus immobilization particle is obtained afterwards three times with physiology salt washing.
7. the method that immobilization fermentation according to claim 5 reduces monascorubin citrinin, it is characterised in that：Step (2) formula of the fermentation medium described in is：Rice meal 5%, wheat bran 0.1%, KNO30.15%, NH4NO30.1%, KH2PO40.3%, K2HPO40.3%, MgSO4·7H2O 0.8%, KCl 0.05%, FeSO4·7H2O 0.001%, ZnSO4·7H2O 0.001%, pH 5.5.
8. the method that immobilization fermentation according to claim 5 reduces monascorubin citrinin, it is characterised in that：Step (2) temperature of fermentation is 25-30 DEG C.
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