CN107929997A - A kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated - Google Patents

A kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated Download PDF

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CN107929997A
CN107929997A CN201710955059.XA CN201710955059A CN107929997A CN 107929997 A CN107929997 A CN 107929997A CN 201710955059 A CN201710955059 A CN 201710955059A CN 107929997 A CN107929997 A CN 107929997A
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rape straw
straw charcoal
parts
polychlorinated biphenyls
solution
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CN107929997B (en
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章春芳
张宁
张冬冬
杨生茂
周航海
张志超
余新威
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/22—Organic substances containing halogen

Abstract

The invention discloses a kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated, and is specially：Polychlorinated biphenyls is placed in rape straw charcoal degraded system with 25mg/L, is cultivated at 30 ± 1 DEG C under dark surrounds more than 30 days, wherein rape straw charcoal degraded system includes following components：Minimal medium 1L, 1.5 2.5ml of sodium lactate, 1 2mL of vitamin solution, 1 2mg of anaerobe, 4 6g of rape straw charcoal.The present invention with rape straw charcoal as solid electronic mediator promotion Polychlorinated biphenyls bio anaerobic reduction dechlorination, rape straw charcoal be naturally be made, be environmental-friendly, raw material sources are easy to get, provide new approach for the degraded of Polychlorinated biphenyls.

Description

A kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated
Technical field
Present invention relates particularly to a kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated, it is related to Organic matter biodegradation field.
Background technology
Polychlorinated biphenyls (Polychlorinated biphenyls, PCBs) is a kind of different degrees of by the hydrogen on biphenyl The general name of chlorinated aromatic compound after being substituted by a chlorine atom both, includes 209 kinds of homologues.Due to its good chemical stabilization Property, anti-flammability and insulating properties, be once widely used in the dielectric of power equipment (such as transformer, variodenser), carbon paper, The PCBs such as ink, coating, lubricant, plasticizer have high toxicity, environmental persistence, bioconcentration, long-distance migration Feature, is difficult to be degraded in the environment since PCBs structures are highly stable, while water-soluble low, fat-soluble height, can be from surrounding It is enriched in media material in organism, and biological accumulation is carried out by the amplification of food chain.It also has half volatile, Can water body or soil by evaporate into atmospheric environment or by adsorbing on particulate matter by way of, pass through atmospheric circulation Remote migration.There is scientist to detect PCBs in the organism of Arctic, toxicological experiment proves that PCBs can be led Cause organism endocrine disturbance, reproduction and immunity function imbalance, development is disorderly or even causes the serious consequences such as cancer, therefore PCBs Pollution causes grave danger to health and ecological environment.In China, once arbitrarily dismounting was useless by someone for Wenzhou District of Zhejiang Province, Taizhou Prefecture Abandon capacitor and cause a large amount of PCBs to be lost in, local air, farmland, river and fish etc. is received serious pollution.In addition, I PCBs is above the 30ngL of U.S. EPA formulation in the water body of state's major part offshore sea waters-1Standard, damage ratio is more serious, and PCBs can be detected in most of marine organisms body.PCBs contents in some deposits of the Huanghai Sea and East Sea nearshore waters Also it is higher.These facts all illustrate that the PCBs pollutions in China have reached certain degree, to ecological environment, animals and humans health Constitute potential threat.Therefore the recovery technique for urgently developing practicality solves the environmental pollution that PCBs is brought.
The method of the degraded of PCBs pollutions at present generally has physical treatment (sealing up for safekeeping, high-temperature process, solvent extraction etc.), chemistry The methods of handling (oxidation-reduction method) and biodegradation, physical or chemical treatment method more or less there are problems that, such as Processing is not thorough, processing cost is too high or produces secondary pollution, these problems limit promoting the use of for physico-chemical process, raw Thing restorative procedure shows its big advantage and potentiality, and biological prosthetic reached by adding contaminant degradation bacterium or relevant enzyme To the effect of pollution administration, such as pollution by polychlorinated biphenyles regional inputs degradation flora biological agent, promote its decomposition and inversion, make it Toxicity is reduced or disappeared.Biodegradation processing currently as it is a kind of most cleaning, most economical processing mode and be considered as most to have Prospect, and most potential method.The technology will not produce secondary pollution, and financial cost is low, easy to implement.
The structural stability and refractory organics of pcb molecule are determined by its chlorination degree, in general chlorination journey Degree is higher, and molecular structure is more stable, is also just more difficult to be degraded.For PCBs, when it is containing more than six chlorine, microorganism can only Chlorion is sloughed by anaerobic reduction process, or converts it into after the PCBs of low chloro and is degraded again by aerobic approach.Therefore, Microbiological anaerobic reduction dechlorination is PCBs degraded committed steps.And the toxicity bigger of the actual high chloro PCBs of points of contamination, it more difficult to It is degraded.
There are many researchs to report that some dehalogenate floras need to rely on the presence of soil or deposit at present, to protect Hold their degrading activity.This phenomenon is dropped in the microorganism of the halo organic pollution such as pentachlorophenol, dioxin and tetrabromobisphenol A Particularly typical case (Zhang, C. are showed in solution；Suzuki,D.；Li,Z.；Ye,L.；Katayama,A.,Polyphasic characterization of two microbial consortia with wide dechlorination spectra for chlorophenols.Journal Of Bioscience And Bioengineering 2012,114,(5),512- 517.Zhang,C.；Katayama,A.,Humin as an Electron Mediator for Microbial Reductive Dehalogenation.Environmental Science&Technology 2012,46,(12),6575- 6583.).Under anaerobic, the reduction dechlorination reaction of most of halo organic pollution is all in soil or deposit Have an effect.It is to promote micro-reduction dehalogenation (more chlorine connection to have been reported that the humin shown in soils and sediments Benzene dechlorination and tetrabromobisphenol A debrominate) occur necessary factor (Zhang, C.；Li,Z.；Suzuki,D.；Ye,L.；Yoshida, N.；Katayama,A.,A humin-dependent Dehalobacter species is involved in reductive debrominationof tetrabromobisphenol A.Chemosphere 2013).In this reaction During humin play the role of electron mediator.We also report humin recently can mediate chlordene biphenyl PCB153 Degraded (Zhang, D., Zhang, N., Yu, X., Zhang, Z., Yang, the S.and of (2,2,4,4,5,5- chlordenes biphenyl) Zhang,C.(2017)Effect of humins from different sediments on microbial degradation of 2,2′,4,4′,5,5′-hexachlorobiphenyl(PCB 153),and their Polyphasic characterization.RSC Advances 7 (12), 6849-6855.), that is to say, that organic contamination Thing, as why the anaerobic biodegradation activity of PCB can only be reacted in soil or deposit, this is because the micro- life of function The humin that thing need to rely in soils and sediments carries out electron transmission, so as to reach the effect of dechlorination.
Humin is the solid organic matters for being both also insoluble in alkali in soil or deposit humus insoluble in acid.At present, learn It is all water miscible material that the electron mediator that persons generally study, which includes humic acid and its pattern compound, in the reality of pollutant It is easy to be lost in during ground is biological prosthetic.It is all insoluble in any pH environment and humin is as solid electronic mediator, and its Chemical constitution is extremely stable, can stay active for long periods.Meanwhile humin or naturally occurring, environmental-friendly, avirulent thing Matter, has very strong application advantage in pollution amelioration on the spot.
But the extracting method of humin is troublesome and time-consuming, extraction and purifying every time needs one or two month. Also, the electronic media effect of humin is often limited by soil or sediment source.
The content of the invention
The present invention provides a kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated, and uses rape straw Charcoal promotes the bio anaerobic reduction dechlorination of Polychlorinated biphenyls as solid electronic mediator, and rape straw charcoal is naturally to make Into, it is environmental-friendly and be widely present in natural environment.
The technical solution adopted by the present invention is：A kind of rape straw charcoal is in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated Using.The application is：Polychlorinated biphenyls is placed in rape straw charcoal degraded system with 25mg/L, dark surrounds at 30 ± 1 DEG C Lower culture more than 30 days.
The rape straw charcoal degraded system includes following components：Minimal medium 1L, sodium lactate 1.5-2.5ml, Vitamin solution 1-2mL, anaerobe 1-2mg, rape straw charcoal 4-6g.
Further, the minimal medium formula is：NH4Cl 1.0g/L, CaCl2·2H2O 0.05g/L, MgCl2·6H2O 0.1g/L, K2HPO40.4g/L, Se/W solution 1mL/L, SL-10 trace element solution 1mL/L, pH7.2's MOPS buffer solution 10mmol/L, vitamin solution 0.02ml/L, water surplus.
Wherein, the composition of every liter of Se/W solution is：NaOH 0.5g, NaSeO3·5H2O 3mg, Na2WO4·2H2O 4mg, Water surplus.
The composition of every liter of SL-10 trace element solution is：HCl 10.0mL, FeCl2·4H2O 1.5g, CoCl2·6H2O 196.0mg, MnCl2·4H2O 100.0mg, ZnCl270.0mg H3BO36.0mg, CuCl2·2H2O 2.0mg, NiCl2· 6H2O 24.0mg, Na2MoO4·2H2O 36.0mg, water surplus.
The composition of every liter of vitamin solution is：Biotin 20mg, folic acid 20mg, VB6100mg, VB150mg, VB2 50mg, VB350mg, D-VB5 calcium 50mg, V B1250mg, p-aminobenzoic acid 50mg, lipoic acid 50mg, VB550mg, it is sub- Iron ferroheme 50mg, 1,2- naphthoquinones 50mg, water surplus.
Further, the anaerobe is mixed by the strain of following mass fraction：Desulfomonas (Desulfuromonassp.) 43.5-45.5 parts, 21.6-25.8 parts of desulfurization bacterium (Desulfitobacteriumsp.) is micro- 3.8-5.3 parts of dialister bacterium (Exiguobacteriumsp.), 6.2-8.1 parts of citric acid bacillus (Citrobactersp.), basophilic 4.7-5.1 parts of bacterium (Alkaliphilussp.), 1.8-2.3 parts of pseudomonad (Pseudomonassp.), dehalogenate bacillus (Dehalobactersp.) 3.5-6.5 parts, sp.3.4-4.1 parts of Bellilinea, Dehalogenimonas sp.2.8-5.9 Part.
Further, the preparation method of the rape straw charcoal is：Rape straw is cut into the branch of 1-2cm long, in charcoal Change in stove, under oxygen free condition, 450 DEG C of temperature, carbonizes 4-6h, takes out, Mechanical Crushing, and grinding, crosses 60 mesh sieves, you can obtains rape Bar charcoal.
The beneficial effects of the invention are as follows：Promote the biology of Polychlorinated biphenyls by the use of rape straw charcoal as solid electronic mediator Anaerobic reductive dechlorination, rape straw charcoal are naturally to be made, is environmental-friendly and be widely present in natural environment, are joined for more chlorine The degraded of benzene provides new approach.
Brief description of the drawings
Fig. 1 is the degradation results figure of embodiment 1.
Fig. 2 is the degradation results figure of embodiment 2.
Fig. 3 is the degradation results figure of embodiment 3.
Fig. 4 is the degradation results figure of comparative example 1.
Fig. 5 is the degradation results figure of comparative example 2.
Embodiment
By studying for a long period of time, inventor is found that a kind of can compare favourably with the effect of the electronic media of humin is imitated in other words The even better solid electronic mediator-rape straw charcoal of fruit.The present invention reports rape straw charcoal as solid electricity first Sub- mediator plays indispensable effect during microbial degradation organo-chlorine pollutant (Polychlorinated biphenyls).It is in view of oily Dish bar charcoal is naturally to be made, is environmental-friendly and be widely present in natural environment, the extracellular electron transport system of this solid Discovery, be organic pollution, particularly persistence organic pollutant (some organic pollutants are easier to be dropped in the environment Solution, can be degraded such as under the action of aerobic bacteria or only need the very short time to be degraded.And as such as pentachlorophenol, more chlorine The organic matters such as biphenyl are difficult to be degraded, and understand and persistently exist in the environment, therefore the microbial degradation utilization of these materials needs spy Fixed condition, need to can just be done by the rape straw charcoal in specific carrier such as this patent) biology in situ recovery technique The important breakthrough of research.
The present invention promotes the bio anaerobic reduction dechlorination of Polychlorinated biphenyls by the use of rape straw charcoal as solid electronic mediator. Rape, originates in China, and Cruciferae, brassica plant, its stem color is dark green, and side such as Chinese cabbage, flower is yellow.Generally it is grown in Place of the weather with respect to moistening.It is mainly distributed on the ground such as Zhejiang, Anhui, Henan, Sichuan.
Promote the work of the bio anaerobic reduction dechlorination of Polychlorinated biphenyls as solid electronic mediator based on rape straw charcoal With, based on rape straw charcoal, the present invention is specific to have prepared the biological agent that can mediate Polychlorinated biphenyls anaerobic reductive dechlorination, Including minimal medium, anaerobe, sodium lactate and vitamin solution, it can especially promote the bio anaerobic of Polychlorinated biphenyls The specific anaerobe selection of reduction dechlorination.The anaerobic reductive dechlorination of Polychlorinated biphenyls is an electronics consumption process, and microorganism is led to Respiration is crossed with acylate (such as sodium lactate) as electron donor, Polychlorinated biphenyls is for electron acceptor by chlorine element from organic pollution The process of middle removal, so the sodium lactate in inventive formulation is added as electron donor.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art. Method in following embodiments, is the conventional method of this area unless otherwise instructed.
Below by specific embodiment, and with reference to attached drawing, technical scheme is described in further detail.
The anaerobe of the present invention is screened from East Sea immediate offshore area bottom sediment sample (122 ° of E, 29 ° of N), through bacterium colony Diversity analysis, microorganism species composition are：Desulfomonas (Desulfuromonassp.), desulfurization bacterium (Desulfitobacteriumsp.) Exiguobacterium sp category (Exiguobacteriumsp.), citric acid bacillus (Citrobactersp.), basophilic bacterium (Alkaliphilussp.), pseudomonad (Pseudomonassp.), dehalogenate bacillus (Dehalobactersp.), Bellilineasp., Dehalogenimonassp..
The component of minimal medium of the present invention, the component of SL-10 trace element solutions, the component of Se/W solution, dimension life The component of plain solution is commercial product.
The MOPS buffers of pH7.2 are：41.8g MOPS are dissolved in 1L distilled water, add the NaOH water of 0.5~1M Solution adjusts pH to 7.2, is then settled to 1L with distilled water, in 4 degrees Celsius of preservations.
The preparation method of rape straw charcoal of the present invention is：Rape straw is cut into the branch of 1cm long, in Muffle furnace, nothing Under the conditions of oxygen, 450 DEG C of temperature, carbonizes 4-6h, takes out, Mechanical Crushing, and grinding, crosses 60 mesh sieves, you can obtains rape straw charcoal.
Embodiment 1：A kind of rape straw charcoal degraded system, is mixed by following components：Minimal medium 1L, detests Oxygen animalcule 1mg, sodium lactate 1.5ml, vitamin solution 1mL, rape straw charcoal 4g.
By mass percentage, the anaerobe is made of following strain：Desulfomonas (Desulfuromonassp.) 43.5%, 23.6% Exiguobacterium sp of desulfurization bacterium (Desulfitobacteriumsp.) (Exiguobacteriumsp.) 3.8%, citric acid bacillus (Citrobactersp.) 6.2%, basophilic bacterium (Alkaliphilussp.) 5.1%, pseudomonad (Pseudomonassp.) 1.8%, dehalogenate bacillus (Dehalobactersp.) 6.5%, Bellilineasp.3.6%, Dehalogenimonassp.5.9%.
The minimal medium formula is：NH4Cl 1.0g/L, CaCl2·2H2O 0.05g/L, MgCl2·6H2O 0.1g/L, K2HPO4The MOPS buffer solutions of 0.4g/L, Se/W solution 1mL/L, SL-10 trace element solution 1mL/L, pH7.2 10mmol/L, vitamin solution 0.02ml/L, water surplus.
The composition of every liter of Se/W solution is：NaOH 0.5g, NaSeO3·5H2O 3mg, Na2WO4·2H2O 4mg, more than water Amount.
The composition of every liter of SL-10 trace element solution is：HCl 10.0mL, FeCl24H2O 1.5g, CoCl2·6H2O 196.0mg, MnCl2·4H2O 100.0mg, ZnCl270.0mg H3BO36.0mg, CuCl22H2O 2.0mg, NiCl2· 6H2O 24.0mg, Na2MoO4·2H2O 36.0mg, water surplus.
The composition of every liter of vitamin solution is：Biotin 20mg, folic acid 20mg, VB6 100mg, VB1 50mg, VB2 50mg, VB3 50mg, D-VB5 calcium 50mg, V B12 50mg, p-aminobenzoic acid 50mg, lipoic acid 50mg, VB5 50mg, it is sub- Iron ferroheme 50mg, 1,2- naphthoquinones 50mg, water surplus.
Embodiment 2：A kind of rape straw charcoal degraded system, is mixed by following components：Minimal medium 1L, detests Oxygen animalcule 2mg, sodium lactate 2.5ml, vitamin solution 2mL, rape straw charcoal 6g.
By mass percentage, the anaerobe is made of following strain：Desulfomonas (Desulfuromonassp.) 45.2%, 25.8% Exiguobacterium sp category of desulfurization bacterium (Desulfitobacteriumsp.) (Exiguobacteriumsp.) 5.3%, citric acid bacillus (Citrobactersp.) 6.5%, basophilic bacterium (Alkaliphilussp.) 4.9%, pseudomonad (Pseudomonassp.) 2.3%, dehalogenate bacillus (Dehalobactersp.) 3.8%, Bellilinea sp.3.4%, Dehalogenimonas sp.2.8%.
The other the same as in Example 1.
Embodiment 3：A kind of rape straw charcoal degraded system, is mixed by following components：Minimal medium 1L, detests Oxygen animalcule 1.5mg, sodium lactate 2ml, vitamin solution 1.5mL, rape straw charcoal 5g.
By mass percentage, the anaerobe is made of following strain：Desulfomonas (Desulfuromonassp.) 45.5%, 21.6% Exiguobacterium sp of desulfurization bacterium (Desulfitobacteriumsp.) (Exiguobacteriumsp.) 5.2%, citric acid bacillus (Citrobactersp.) 8.1%, basophilic bacterium (Alkaliphilussp.) 4.7%, pseudomonad (Pseudomonassp.) 1.8%, dehalogenate bacillus (Dehalobactersp.) 3.5%, Bellilinea sp.4.1%, Dehalogenimonas sp.5.5%.
The other the same as in Example 1.
Comparative example 1：Anaerobe is not added with, remaining is the same as embodiment 1.
Comparative example 2：Rape straw charcoal is not added with, remaining is the same as embodiment 1.
Polychlorinated biphenyls is placed in the degraded system that embodiment 1-3 and comparative example 1-2 are prepared with 25mg/L, 30 ± Degrading experiment is carried out at 1 DEG C under dark surrounds, after culture at least 30 days, is taken with disposable syringe from cultivating system 1ml samples, and (ratio is 1 using n-hexane and acetone:9) PCB and its metabolite are therefrom extracted for extractant, then with outfit 7000 Series gas chromatographs of Agilent-mass spectrometry-mass spectrometry of HP-5MS capillary columns (J＆W Scientific, Folsom, CA) Combined instrument (GC-MS-MS) (U.S., Agilent) carries out sample analysis.
Agilent 7000GC-MS-MS program technics are：80 DEG C of initial temperature, keeps 2min, 196 is risen to 8 DEG C/min DEG C, then the speed of 2 DEG C/min rises to 228 DEG C, then rises to 250 DEG C with the speed of 8 DEG C/min, 12min is kept, finally with 10 DEG C/speed of min rises to 290 DEG C, 3min is kept, amounts to 54.25min.
Quantified by using PCB standard items, this buys the Aroclor1260 used (PCB-3) containing trichloro biphenyl respectively 1.98mol%, tetrachloro biphenyl (PCB-4) 4.23mol%, pentachlorodiphenyl (PCB-5) 32.71mol%, chlordene biphenyl (PCB-6) 32.67mol%, heptachlor biphenyl (PCB-7) 25.46mol%, eight chlordiphenyls (PCB-8) 2.95mol%.
Fig. 1 shows the degradation results of embodiment 1, detest system culture 30 days by above-mentioned culture, after 60 days, 75 days, The high chloro Polychlorinated biphenyls PCB-5 of main component, PCB-6, PCB-7 are substantially completely degraded in Aroclor, are progressively converted into low toxicity PCB-3 and PCB-4, wherein, from the 0th day to the 60th day, PCB-8 from 2.95mol% to 2.62mol%, PCB-7 from 25.46mol% to 13.2mol%, PCB-6 from 32.67mol% to 13.13mol%, PCB-5 from 32.71mol% to 10.28mol%, PCB-4 are from 4.23mol% to 46.46mol%, and PCB-3 is from 1.98mol% to 15.06mol%, 60-75 Its degradation effect is basicly stable.
Fig. 2 represents the degradation results of embodiment 2, detests system culture 30 days by above-mentioned culture, after 60 days, 75 days, The high chloro Polychlorinated biphenyls PCB-5 of main component, PCB-6, PCB-7 are substantially completely degraded in Aroclor, are progressively converted into low toxicity PCB-3 and PCB-4, wherein, from the 0th day to the 60th day, PCB-8 from 2.95mol% to 2.48mol%, PCB-7 from 25.46mol% to 5.15mol%, PCB-6 from 32.67mol% to 3.89mol%, PCB-5 from 32.71mol% to 5.39mol%, PCB-4 are from 4.23mol% to 62.62mol%, and PCB-3 is from 1.98mol% to 20.97mol%, the 60-75 days Degradation effect is basicly stable.
Fig. 3 represents the degradation results of embodiment 3, detests system culture 30 days by above-mentioned culture, after 60 days, 75 days, The high chloro Polychlorinated biphenyls PCB-5 of main component, PCB-6, PCB-7 are substantially completely degraded in Aroclor, are progressively converted into low toxicity PCB-3 and PCB-4, wherein, from the 0th day to the 60th day, PCB-8 from 2.95mol% to 2.63mol%, PCB-7 from 25.46mol% to 8.35mol%, PCB-6 from 32.67mol% to 9.87mol%, PCB-5 from 32.71mol% to 8.25mol%, PCB-4 are from 4.23mol% to 58.08mol%, and PCB-3 is from 1.98mol% to 18.82mol%, the 60-75 days Degradation effect is basicly stable.
Fig. 4 represents the degradation results of comparative example 1, adds rape straw charcoal, is not added with anaerobe, Polychlorinated biphenyls is compared Do not have degraded substantially in standard sample, illustrate the participation of the necessary anaerobe of anaerobic degradation of Polychlorinated biphenyls.
Fig. 5 represents the degradation results of comparative example 2, is not added with rape straw charcoal, and only plus anaerobe, Polychlorinated biphenyls are basic And do not degrade, illustrate that the degraded of Polychlorinated biphenyls is necessarily required to the participation of rape straw charcoal, rape straw charcoal can replace yet The deoxidization, degradation experiment of soil or humin as electron mediator mediation Polychlorinated biphenyls.
Experiment shows that this biological agent can be most of high chloro Polychlorinated biphenyls (PCB-5, PCB-6, PCB-7, PCB-8) Dechlorination becomes low chloro Polychlorinated biphenyls (PCB-3, PCB-4), can significantly reduce its toxicity and degraded difficulty, make it easier into one Step is degraded by aerobic approach.
In addition, the present invention is also using other different charcoal (rubbish biological charcoal, peanut hull biologic charcoal, hickory nut biologies Charcoal etc.) replace rape straw charcoal to carry out degradation experiment, almost do not have degradation effect to Polychlorinated biphenyls Aroclor1260.Speculate, oil The component of dish bar charcoal may the active group containing the unexistent tool electron transfer capacity of other biological charcoal, which can be The electronics produced in oxidation-reduction process in culture systems is transferred to Polychlorinated biphenyls under the action of microorganism, so as to mediate more chlorine The anaerobic organism reduction dechlorination of biphenyl.
Embodiment described above is a kind of preferable scheme of the present invention, and not the present invention is made in any form Limitation, also has other variations and remodeling on the premise of without departing from the technical solution described in claim.

Claims (5)

  1. A kind of 1. application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated.
  2. 2. application according to claim 1, it is characterised in that the application is：Polychlorinated biphenyls is placed in oil with 25mg/L In dish bar charcoal degraded system, cultivated more than 30 days under dark surrounds at 30 ± 1 DEG C.
    The rape straw charcoal degraded system includes following components：Minimal medium 1L, sodium lactate 1.5-2.5ml, dimension life Plain solution 1-2mL, anaerobe 1-2mg, rape straw charcoal 4-6g.
  3. 3. application according to claim 2, it is characterised in that the minimal medium formula is：NH4Cl 1.0g/L, CaCl2·2H2O 0.05g/L, MgCl2·6H2O 0.1g/L, K2HPO4The micro members of 0.4g/L, Se/W solution 1mL/L, SL-10 The MOPS buffer solution 10mmol/L of plain solution 1mL/L, pH7.2, vitamin solution 0.02ml/L, water surplus.
    Wherein, the composition of every liter of Se/W solution is：NaOH 0.5g, NaSeO3·5H2O 3mg, Na2WO4·2H2O 4mg, more than water Amount.
    The composition of every liter of SL-10 trace element solution is：HCl 10.0mL, FeCl2·4H2O 1.5g, CoCl2·6H2O 196.0mg, MnCl2·4H2O 100.0mg, ZnCl270.0mg H3BO36.0mg, CuCl2·2H2O 2.0mg, NiCl2· 6H2O 24.0mg, Na2MoO4·2H2O 36.0mg, water surplus.
    The composition of every liter of vitamin solution is：Biotin 20mg, folic acid 20mg, VB6100mg, VB150mg, VB250mg, VB3 50mg, D-VB5 calcium 50mg, V B1250mg, p-aminobenzoic acid 50mg, lipoic acid 50mg, VB550mg, heme 50mg, 1,2- naphthoquinones 50mg, water surplus.
  4. 4. application according to claim 2, it is characterised in that the anaerobe is mixed by the strain of following mass fraction Conjunction is made：43.5-45.5 parts of Desulfomonas (Desulfuromonassp.), desulfurization bacterium (Desulfitobacteriumsp.) 21.6-25.8 parts, 3.8-5.3 parts of Exiguobacterium sp (Exiguobacteriumsp.), lemon 6.2-8.1 parts of acidfast bacilli (Citrobactersp.), 4.7-5.1 parts of basophilic bacterium (Alkaliphilussp.), pseudomonad (Pseudomonassp.) 1.8-2.3 parts, 3.5-6.5 parts of dehalogenate bacillus (Dehalobactersp.), Bellilinea Sp.3.4-4.1 parts, sp.2.8-5.9 parts of Dehalogenimonas.
  5. 5. application according to claim 2, it is characterised in that the preparation method of the rape straw charcoal is：By rape Bar is cut into the branch of 1-2cm long, and in retort, under oxygen free condition, 450 DEG C of temperature, carbonizes 4-6h, takes out, Mechanical Crushing, grinds Mill, crosses 60 mesh sieves, you can obtains rape straw charcoal.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113582328A (en) * 2021-08-20 2021-11-02 浙江大学 Method for strengthening anaerobic dechlorination of polychlorinated biphenyl microorganisms

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101428171A (en) * 2008-07-25 2009-05-13 东华大学 Degradation method for polychlorinated biphenyl with staphylococcus epidermidis
CN102512783A (en) * 2011-10-19 2012-06-27 中国科学院烟台海岸带研究所 Method for high-efficiency degradation of persistent organic chloridized pollutant
US20130060076A1 (en) * 2011-09-01 2013-03-07 Keith E. Forrester Method to reduce pcb content and/or lead tclp solubility
CN103436460A (en) * 2013-06-24 2013-12-11 浙江大学 Method for enriching efficient biphenyl degradation flora by using accelerator SRpf
CN103740593A (en) * 2013-12-28 2014-04-23 杭州师范大学 Anabaena capable of efficiently degrading polychlorinated biphenyl and application thereof
CN105344057A (en) * 2015-10-28 2016-02-24 浙江大学 New application of caragana microphylla charcoal and mediate chlorophenasic acid anaerobic reductive dechlorination process thereof
CN105413094A (en) * 2015-10-28 2016-03-23 浙江省农业科学院 Biochar preparation capable of mediating in anaerobic reductive dechlorination of chlorophenasic acid
CN105689386A (en) * 2016-02-29 2016-06-22 华南理工大学 Reinforcing method for degrading polychlorinated biphenyl in soil or sediment

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101428171A (en) * 2008-07-25 2009-05-13 东华大学 Degradation method for polychlorinated biphenyl with staphylococcus epidermidis
US20130060076A1 (en) * 2011-09-01 2013-03-07 Keith E. Forrester Method to reduce pcb content and/or lead tclp solubility
CN102512783A (en) * 2011-10-19 2012-06-27 中国科学院烟台海岸带研究所 Method for high-efficiency degradation of persistent organic chloridized pollutant
CN103436460A (en) * 2013-06-24 2013-12-11 浙江大学 Method for enriching efficient biphenyl degradation flora by using accelerator SRpf
CN103740593A (en) * 2013-12-28 2014-04-23 杭州师范大学 Anabaena capable of efficiently degrading polychlorinated biphenyl and application thereof
CN105344057A (en) * 2015-10-28 2016-02-24 浙江大学 New application of caragana microphylla charcoal and mediate chlorophenasic acid anaerobic reductive dechlorination process thereof
CN105413094A (en) * 2015-10-28 2016-03-23 浙江省农业科学院 Biochar preparation capable of mediating in anaerobic reductive dechlorination of chlorophenasic acid
CN105689386A (en) * 2016-02-29 2016-06-22 华南理工大学 Reinforcing method for degrading polychlorinated biphenyl in soil or sediment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113582328A (en) * 2021-08-20 2021-11-02 浙江大学 Method for strengthening anaerobic dechlorination of polychlorinated biphenyl microorganisms

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