CN105344057A - New application of caragana microphylla charcoal and mediate chlorophenasic acid anaerobic reductive dechlorination process thereof - Google Patents

New application of caragana microphylla charcoal and mediate chlorophenasic acid anaerobic reductive dechlorination process thereof Download PDF

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CN105344057A
CN105344057A CN201510710894.8A CN201510710894A CN105344057A CN 105344057 A CN105344057 A CN 105344057A CN 201510710894 A CN201510710894 A CN 201510710894A CN 105344057 A CN105344057 A CN 105344057A
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caragana microphylla
biological charcoal
charcoal
microphylla biological
pentachlorophenol
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CN105344057B (en
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章春芳
杨生茂
钟哲科
刘玉学
李智灵
张冬冬
吕豪豪
汪玉瑛
张宁
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/22—Organic substances containing halogen
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen

Abstract

The invention discloses a new application of caragana microphylla charcoal and a mediate chlorophenasic acid anaerobic reductive dechlorination process thereof. The caragana microphylla charcoal serves as a solid electronic medium for mediate chlorophenasic acid anaerobic reductive dechlorination. The caragana microphylla charcoal serves as the solid electronic medium, so that biological anaerobic reductive dechlorination of chlorophenasic acid is promoted; and the caragana microphylla charcoal is naturally made, is environment-friendly and widely exists in the natural environment.

Description

A kind of novelty teabag of Caragana Microphylla biological charcoal and the technique of mediation pentachlorophenol anaerobic reductive dechlorination thereof
Technical field
The present invention relates to organic matter biodegradation field, particularly a kind of novelty teabag of Caragana Microphylla biological charcoal and the technique of mediation pentachlorophenol anaerobic reductive dechlorination thereof.
Background technology
Halogenated aromatic compound and derivative thereof are the industrial chemicals of the industry extensive uses such as chemical industry, medicine, process hides, electronics, organic synthesis intermediate and organic solvent.Because its pollution is wide, toxicity is degraded greatly, not easily, and mostly can produce teratogenesis, carcinogenic, mutagenesis " three cause " effect to human body, thus cause and pay close attention to widely.Wherein, pentachlorophenol (Pentachlorophenol, PCP) is a kind of typical halogenated aromatic compound, and Zeng Zuowei pesticide, timber preservative and mould inhibitor etc. are widely used, thus cause worldwide soil and groundwater to pollute.Because PCP brings serious threat to human health (to liver and kidney tool high toxicity and have cause cancer equivalent risk) and ecological environment, therefore in last century the eighties be prohibited from using.At present, PCP is still identified as persistence organic pollutant, and China, the U.S. and world health organization are all classified as priority pollutants.
The structural stability of chloro organic cpd molecule and refractory organics are determined by its chlorination degree, and in general chlorination degree is higher, and molecular structure is more stable, are also more difficultly degraded.For most of chlorinated aromatic organic compound, the distich R-Cl/R-H of its standard oxidationreduction potential is between 250mV ~ 600mV.Therefore, these compounds not easily pass through Aerobic Process for Treatment and are degraded, and but under anaerobic can carry out reduction and transform.Therefore, microbiological anaerobic reduction dechlorination is the first step of PCP degraded is also a most important step.
Inventor studies discovery, under must having soil or deposit existent condition, just can carry out anaerobic reductive dechlorination reaction (Zhang, the C. of pentachlorophenol in microorganism cultivating system; Suzuki, D.; Li, Z.; Ye, L.; Katayama, A., Polyphasiccharacterizationoftwomicrobialconsortiawithwid edechlorinationspectraforchlorophenols. journalOfBioscienceAndBioengineering2012, 114, (5), 512-517.Zhang, C.; Katayama, A., HuminasanElectronMediatorforMicrobialReductiveDehalogena tion. environmentalScience & Technology2012, 46, (12), 6575-6583.).By By consulting literatures, we find that this situation is not special case.A lot of some dehalogenate flora of research report has been had to need to depend on soil or sedimental existence, to keep their degrading activity at present.This phenomenon shows particularly typical case in the microbial degradation of the halo organic pollutions such as Polychlorinated biphenyls, dioxin and tetrabromobisphenol A.Under anaerobic, the reduction dechlorination reaction of most of halo organic pollution is all had an effect in soil or deposit.For a long time, the concrete effect that soil or deposit play in micro-reduction dehalogenate process never has breakthrough.In nearest research, the humin that we report in soils and sediments is the necessary factor (Zhang, the C. that promote that micro-reduction dehalogenation (pentachlorophenol dechlorination and tetrabromobisphenol A debrominate) occurs; Li, Z.; Suzuki, D.; Ye, L.; Yoshida, N.; Katayama, A., Ahumin-dependent dehalobacterspeciesisinvolvedinreductivedebrominationoftetrabromobis phenolA. chemosphere2013).In this course of reaction, humin serves the effect of electron mediator.That is, organic pollution, why anaerobic biodegradation activity as PCP can only react in soil or deposit, and this is because functional microorganism needs to depend on humin in soils and sediments to carry out electron transmission, thus reaches the effect of dechlorination.
Humin had both been insoluble to the solid organic matters that acid is also insoluble to alkali in soil or deposit humus.At present, the electron mediator that scholars generally study comprises humic acid and medelling compound is all water miscible material, pollutant biological prosthetic on the spot in be easy to run off.And humin is as solid electronic mediator, do not dissolve in any pH environment, and its chemical constitution is extremely stable, can stay active for long periods.Meanwhile, humin or natural existence, environmental friendliness, avirulent material, have very strong application advantage on the spot in pollution amelioration.
But the very loaded down with trivial details and length consuming time of the extracting method of humin, to extract and purifying needs one or two month at every turn.Further, the electronic media effect of humin is often subject to the restriction of soil or sediment source.
Summary of the invention
The object of the present invention is to provide a kind of novelty teabag of Caragana Microphylla biological charcoal, promote the bio anaerobic reduction dechlorination of pentachlorophenol with Caragana Microphylla biological charcoal as solid electronic mediator, Caragana Microphylla biological charcoal be naturally to make, environmental friendliness and extensively existing in natural environment.
Present invention also offers the technique of a kind of Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination, raw material sources are easy to get, and can effectively mediate pentachlorophenol anaerobic reductive dechlorination, the degraded for pentachlorophenol provides new approach.
The technical solution adopted for the present invention to solve the technical problems is:
A novelty teabag for Caragana Microphylla biological charcoal, Caragana Microphylla biological charcoal is as the purposes of the solid electronic mediator of mediation pentachlorophenol anaerobic reductive dechlorination.
Through studying for a long period of time, inventor finds a kind of can comparing favourably the even better solid electronic mediator-Caragana Microphylla biological charcoal of effect in other words with the electronic media effect of humin.Reported first Caragana Microphylla biological charcoal of the present invention plays a part indispensable as solid electronic mediator in the process of microbial degradation organo-chlorine pollutant (pentachlorophenol).In view of Caragana Microphylla biological charcoal be naturally to make, environmental friendliness and extensively existing in natural environment, the discovery of this solid born of the same parents exoelectron transport system, it is organic pollution, (some organic pollutants is degraded than being easier in the environment, as being degraded under the effect of aerobic bacteria or only needing the very short time to be degraded in the important breakthrough of particularly persistence organic pollutant biology in situ recovery technique research.And the picture such as organic matter such as PCP, Polychlorinated biphenyls is difficult to be degraded, can exist lastingly in the environment, therefore the microbial degradation of these materials utilizes and needs specific condition, need by specific carrier as the Caragana Microphylla biological charcoal in this patent just can be done).
The present invention promotes the bio anaerobic reduction dechlorination of pentachlorophenol as solid electronic mediator with Caragana Microphylla biological charcoal.Caragana microphylla, large shrub forage plant of falling leaves, root system is very flourishing, and main root buries deeply, and plant height is 40-70 centimetre, reaches as high as about 2 meters.Drought-enduring, cold-resistant, high temperature resistant, be the raw shrubbery of drought in aridity grass land, desert steppe area.Be one of the important species of NW China, North China, West of The Northeast China water and soil conservation and dune-fixating forestation, belong to excellent and fix the sand and planting trees on barren hills plant, good forage grass and feed.
As preferably, the preparation method of described Caragana Microphylla biological charcoal is: caragana microphylla is cut into the branch that 5-10cm is long, and in retort, under oxygen free condition, temperature 400-500 DEG C, charing 4-6h, takes out, Mechanical Crushing, and grinding, can obtain Caragana Microphylla biological charcoal.
A technique for Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination, is placed in Caragana Microphylla biological charcoal degraded system, cultivates more than 10 days at 30 ± 1 DEG C under dark surrounds by pentachlorophenol.
As preferably, described Caragana Microphylla biological charcoal degraded system is mixed by following component: minimal medium 1L, anaerobe 0.5-1mg, sodium formate 0.6-0.8g, vitamin solution 1-2mL, Caragana Microphylla biological charcoal 4-6g.
Promote the effect of the bio anaerobic reduction dechlorination of pentachlorophenol as solid electronic mediator based on Caragana Microphylla biological charcoal, based on Caragana Microphylla biological charcoal, specific development of the present invention can mediate the technique of pentachlorophenol anaerobic reductive dechlorination, degraded system comprises minimal medium, anaerobe, sodium formate and vitamin solution and Caragana Microphylla biological charcoal, especially can promote that the specific anaerobe of the bio anaerobic reduction dechlorination of pentachlorophenol is selected.The anaerobic reductive dechlorination of PCP is an electronics consumption process, microorganism by respiration with organic acid (as formic acid) for electron donor, PCP is the process that chlorine element is removed by electron acceptor from organic pollution, so the sodium formate in the present invention's formula adds as electron donor.
As preferably, consisting of of described minimal medium: NH 4cl1.0g/L, CaCl 22H 2o0.05g/L, MgCl 26H 2o0.1g/L, K 2hPO 40.4g/L, SL-10 trace element solution 1mL/L, the MOPs buffer solution 15mmol/L of Se/W solution 1mL/L, pH7.2, water surplus.
As preferably, consisting of of often liter of SL-10 trace element solution: HCl10.0mL, FeCl 24H 2o1.5g, ZnCl 270.0mg, MnCl 24H 2o100.0mg, H 3bO 36.0mg, CoCl 26H 2o196.0mg, CuCl 22H 2o2.0mg, NiCl 26H 2o24.0mg, Na 2moO 42H 2o36.0mg, water surplus.
As preferably, consisting of of often liter of Se/W solution: NaOH0.5g, NaSeO 35H 2o3mg, Na 2wO 42H 2o4mg, water surplus.
As preferably, by mass percentage, described anaerobe is mixed by following bacterial classification: sulphur monad 38-40%, bacteroid 31-32%, Clostridium bifermentans 19-20%, dehalogenate bacillus 4-6%, desulfovibrio 4-6%.Specific anaerobe composition of the present invention effectively can promote the bio anaerobic reduction dechlorination of pentachlorophenol.
As preferably, consisting of of often liter of vitamin solution: biotin 20mg, folic acid 20mg, VB 6100mg, VB 150mg, VB 250mg, VB 350mg, D-VB5 calcium 50mg, VB 1250mg, p-aminobenzoic acid 50mg, lipoic acid 50mg, VB 550mg, heme 50mg, 1,2-naphthoquinones 50mg, water surplus.
The invention has the beneficial effects as follows:
1, promote the bio anaerobic reduction dechlorination of pentachlorophenol as solid electronic mediator with Caragana Microphylla biological charcoal, Caragana Microphylla biological charcoal be naturally to make, environmental friendliness and extensively existing in natural environment.
2, the technique of Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination of the present invention, raw material sources are easy to get, and can effectively mediate pentachlorophenol anaerobic reductive dechlorination, the degraded for pentachlorophenol provides new approach.
Accompanying drawing explanation
Fig. 1 is the degradation results figure that Caragana Microphylla biological charcoal and anaerobe add jointly.
Fig. 2 is the degradation results figure that Caragana Microphylla biological charcoal or anaerobe add separately.
Detailed description of the invention
Below by specific embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.
In the present invention, if not refer in particular to, the raw material adopted and equipment etc. all can be buied from market or this area is conventional.Method in following embodiment, if no special instructions, is the conventional method of this area.
Anaerobe of the present invention is commercially available bacterial classification, sulphur monad ( sulfurospirillumhalorespiransdSM13726), bacteroid ( bacteroidesgraminisolventsdSM19988), Clostridium bifermentans ( clostridiumbifermentansaTCC638), dehalogenate bacillus ( dehalobacterrestrictusdSM9455), desulfovibrio ( desulfovibriomarrakechensisdSM19337).Wherein DSM13726, DSM19988, DSM9455 etc. are the strain numbers of German Culture Collection (DSMZ); ATCC638 is the numbering at american strain preservation center.
The composition of minimal medium of the present invention, the composition of SL-10 trace element solution, the composition of Se/W solution, the composition of vitamin solution is all commercially available prod.
The MOPS buffer of pH7.2 is: 41.8gMOPS is dissolved in 1L distilled water, and the NaOH aqueous solution adding 0.5 ~ 1M regulates pH to 7.2, is then settled to 1L with distilled water, in 4 degrees Celsius of preservations.
The preparation method of Caragana Microphylla biological charcoal of the present invention is: caragana microphylla is cut into the branch that 5-10cm is long, and in retort, under oxygen free condition, temperature 400-500 DEG C, charing 4-6h, takes out, Mechanical Crushing, and grinding, can obtain Caragana Microphylla biological charcoal.
A novelty teabag for Caragana Microphylla biological charcoal, Caragana Microphylla biological charcoal is as the purposes of the solid electronic mediator of mediation pentachlorophenol anaerobic reductive dechlorination.
Embodiment 1:
A kind of technique of Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination, pentachlorophenol (PCP) is placed in Caragana Microphylla biological charcoal degraded system (general degradable PCP be add degraded system final concentration be 20-50 μm of ol/L), cultivates more than 10 days under dark surrounds at 30 ± 1 DEG C.Caragana Microphylla biological charcoal degraded system is mixed by following component: minimal medium 1L, anaerobe 0.5mg, sodium formate 0.6g, vitamin solution 1mL, Caragana Microphylla biological charcoal 4g.
By mass percentage, described anaerobe is mixed by following bacterial classification: sulphur monad 38%, bacteroid 32%, Clostridium bifermentans 20%, dehalogenate bacillus 4%, desulfovibrio 6%.
Consisting of of minimal medium: NH 4cl1.0g/L, CaCl 22H 2o0.05g/L, MgCl 26H 2o0.1g/L, K 2hPO 40.4g/L, SL-10 trace element solution 1mL/L, the MOPs buffer solution 15mmol/L of Se/W solution 1mL/L, pH7.2, water surplus.
Consisting of of often liter of SL-10 trace element solution: HCl(7.7M) 10.0mL, FeCl 24H 2o1.5g, ZnCl 270.0mg, MnCl 24H 2o100.0mg, H 3bO 36.0mg, CoCl 26H 2o196.0mg, CuCl 22H 2o2.0mg, NiCl 26H 2o24.0mg, Na 2moO 42H 2o36.0mg, water surplus.
Consisting of of often liter of Se/W solution: NaOH0.5g, NaSeO 35H 2o3mg, Na 2wO 42H 2o4mg, water surplus.
Consisting of of often liter of vitamin solution: biotin 20mg, folic acid 20mg, VB 6100mg, VB 150mg, VB 250mg, VB 350mg, D-VB5 calcium 50mg, VB 1250mg, p-aminobenzoic acid 50mg, lipoic acid 50mg, VB 550mg, heme 50mg, 1,2-naphthoquinones 50mg, water surplus.
Embodiment 2:
A technique for Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination, is placed in Caragana Microphylla biological charcoal degraded system, cultivates more than 10 days at 30 ± 1 DEG C under dark surrounds by pentachlorophenol (PCP).Caragana Microphylla biological charcoal degraded system is mixed by following component: minimal medium 1L, anaerobe 1mg, sodium formate 0.8g, vitamin solution 2mL, Caragana Microphylla biological charcoal 6g.
By mass percentage, described anaerobe is mixed by following bacterial classification: sulphur monad 40%, bacteroid 31%, Clostridium bifermentans 19%, dehalogenate bacillus 6%, desulfovibrio 4%.
The other the same as in Example 1.
Embodiment 3:
A technique for Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination, is placed in Caragana Microphylla biological charcoal degraded system, cultivates more than 10 days at 30 ± 1 DEG C under dark surrounds by pentachlorophenol (PCP).Caragana Microphylla biological charcoal degraded system is mixed by following component: minimal medium 1L, anaerobe 0.8mg, sodium formate 0.69g, vitamin solution 1mL, Caragana Microphylla biological charcoal 5g.
By mass percentage, described anaerobe is mixed by following bacterial classification: sulphur monad 39.2%, bacteroid 31.7%, Clostridium bifermentans 19.5%, dehalogenate bacillus 4.8%, desulfovibrio 4.8%.
The other the same as in Example 1.
Degrading experiment:
In the serum bottle of 60mL, add 20mL minimal medium and 0.1g Caragana Microphylla biological charcoal (final concentration is exactly 5g/L), in bottle, rushing nitrogen afterwards, (purity is 99.99%.The degraded of PCP under anaerobic completes.Rushing nitrogen is in order to the oxygen in culture medium is driven away, preparation anaerobic culture medium), there are rubber stopper (Teflon-coatedbutylrubberstoppers, internal diameter 13mm, the production of wheaton company of the U.S. of polytetrafluorethylecoatings coatings after about 30 minutes beyond the Great Wall.Specially use and have the rubber stopper of polytetrafluorethylecoatings coatings to be because General Purpose Rubber plug can adsorb the organic matter of such as PCP etc., rubber stopper scribbles the absorption that polytetrafluoroethylene (PTFE) can prevent PCP, experimental result is better explained), then cover aluminium lid and seal, finally moist heat sterilization 30 minutes under 121 DEG C of conditions.
When carrying out degradation experiment, 0.02mL vitamin solution and 2mmol/LNa is added by 0.2 μm of molecular filter (application target: just in case have growth of microorganism in vitamin solution and reductant solution, filter membrane can prevent it from entering in cultivating system) in the bottle of above-mentioned bacterium of having gone out 2s9H 2o(reducing agent, guarantees the anaerobic condition in culture medium.The Na of prior preparation 200mmol/L 2s9H 2o storing solution, is stored in 4 degree of refrigerators after autoclaving.Get in 200 μ l to 20ml culture medium systems during degradation experiment from storing solution, to make its final concentration for 2mmol/L), then add 20 μMs of PCP(PCP and be dissolved in acetone with the form of PCP-Na salt, what join is the storing solution of 5mmol/L.Get in 80 μ l to 20mL culture medium systems when doing degradation experiment from storing solution, be 20 μm of ol/L to make its final concentration) and 10mmol/L sodium formate (sodium formate solution is dissolved in water with the form of sodium formate, what join is the storing solution of 1mol/L, prior autoclaving.Get in 200 μ l to 20ml culture medium systems during degradation experiment from storing solution, to make its final concentration for 10mmol/L), finally access the anaerobe of 0.02mg.
Above-mentioned culture to be placed in incubator at 30 DEG C quiescent culture in dark surrounds.After cultivation at least 10 days, from cultivating system, 1ml sample is taked with disposable syringe, and with acetonitrile and ethyl acetate for extractant therefrom extracts PCP and metabolite thereof, then with being equipped with DB-5MS capillary column (J & WScientific, Folsom, CA) QP5050 GC-MS (Shimadzu, capital of a country, Japan) carries out sample analysis.
Degrading experiment is divided into three groups:
1st group: add Caragana Microphylla biological charcoal+anaerobe (minimal medium+Caragana Microphylla biological charcoal+anaerobe+vitamin solution+sodium formate+PCP); 2nd group: add Caragana Microphylla biological charcoal, do not add anaerobe (minimal medium+Caragana Microphylla biological charcoal+vitamin solution+sodium formate+PCP); 3rd group: do not add Caragana Microphylla biological charcoal, add anaerobe (minimal medium+vitamin solution+sodium formate+PCP).All experimental group are all provided with Duplicate Samples, and often group is tested at least in triplicate.
Experimental result:
Fig. 1 represents the 1st group of situation of testing, and cultivate 15 days by above-mentioned cultivating system, pentachlorophenol all realizes anaerobic reductive dechlorination, and generating phenol is final metabolite, and namely phenol content reaches maximum.In the cultivating system of 20ml, again add the PCP of 20 μMs when the 15th day, continue cultivation after 30 days, pentachlorophenol all realizes anaerobic reductive dechlorination, and its content reduces to 0 μM, and end-product phenol content reaches maximum simultaneously.Continued to add pentachlorophenol in cultivating system at the 45th day, experimental result display two weeks rear PCP are all reduced dechlorination again, show that the anaerobic reductive dechlorination activity of this microorganism system is very stable.Fig. 2 represents the 2nd group, the control experiment result of the 3rd group, and finding has separately Caragana Microphylla biological charcoal or anaerobe to deposit in case, does not almost have degradation effect to pentachlorophenol, and cultivate 15 days respectively, 25 days, after 38 days, the content of pentachlorophenol was substantially constant.This description of test Caragana Microphylla biological charcoal can mediate the anaerobic reductive dechlorination effect of pentachlorophenol really, and under not having Caragana Microphylla biological charcoal existent condition, the dechlorination reaction of pentachlorophenol does not carry out.
In addition, the present invention also adopts other different charcoal (rubbish biological charcoal, bamboo charcoal, corncob charcoal etc.) to replace Caragana Microphylla biological charcoal to carry out degradation experiment, does not almost have degradation effect to pentachlorophenol.Infer, Caragana Microphylla biological charcoal may contain the active group of the unexistent tool electron transfer capacity of other biological charcoal, this group can under the effect of microorganism by the electro transfer that produces in oxidation-reduction process in culture systems to pentachlorophenol, thus the anaerobic organism reduction dechlorination of mediation pentachlorophenol.
Above-described embodiment is one of the present invention preferably scheme, not does any pro forma restriction to the present invention, also has other variant and remodeling under the prerequisite not exceeding the technical scheme described in claim.

Claims (9)

1. a novelty teabag for Caragana Microphylla biological charcoal, is characterized in that: Caragana Microphylla biological charcoal is as the purposes of the solid electronic mediator of mediation pentachlorophenol anaerobic reductive dechlorination.
2. the novelty teabag of a kind of Caragana Microphylla biological charcoal according to claim 1, it is characterized in that: the preparation method of described Caragana Microphylla biological charcoal is: caragana microphylla is cut into the branch that 5-10cm is long, in retort, under oxygen free condition, temperature 400-500 DEG C, charing 4-6h, take out, Mechanical Crushing, grinding, can obtain Caragana Microphylla biological charcoal.
3. a technique for Caragana Microphylla biological charcoal mediation pentachlorophenol anaerobic reductive dechlorination, is characterized in that: pentachlorophenol is placed in Caragana Microphylla biological charcoal degraded system, cultivates more than 10 days at 30 ± 1 DEG C under dark surrounds.
4. technique according to claim 3, it is characterized in that: described Caragana Microphylla biological charcoal degraded system is mixed by following component: minimal medium 1L, anaerobe 0.5-1mg, sodium formate 0.6-0.8g, vitamin solution 1-2mL, Caragana Microphylla biological charcoal 4-6g.
5. technique according to claim 4, is characterized in that: consisting of of described minimal medium: NH 4cl1.0g/L, CaCl 22H 2o0.05g/L, MgCl 26H 2o0.1g/L, K 2hPO 40.4g/L, SL-10 trace element solution 1mL/L, the MOPs buffer solution 15mmol/L of Se/W solution 1mL/L, pH7.2, water surplus.
6. technique according to claim 5, is characterized in that: consisting of of often liter of SL-10 trace element solution: HCl10.0mL, FeCl 24H 2o1.5g, ZnCl 270.0mg, MnCl 24H 2o100.0mg, H 3bO 36.0mg, CoCl 26H 2o196.0mg, CuCl 22H 2o2.0mg, NiCl 26H 2o24.0mg, Na 2moO 42H 2o36.0mg, water surplus.
7. technique according to claim 5, is characterized in that: consisting of of often liter of Se/W solution: NaOH0.5g, NaSeO 35H 2o3mg, Na 2wO 42H 2o4mg, water surplus.
8. technique according to claim 4, is characterized in that: by mass percentage, and described anaerobe is mixed by following bacterial classification: sulphur monad 38-40%, bacteroid 31-32%, Clostridium bifermentans 19-20%, dehalogenate bacillus 4-6%, desulfovibrio 4-6%.
9. technique according to claim 4, is characterized in that: consisting of of often liter of vitamin solution: biotin 20mg, folic acid 20mg, VB 6100mg, VB 150mg, VB 250mg, VB 350mg, D-VB5 calcium 50mg, VB 1250mg, p-aminobenzoic acid 50mg, lipoic acid 50mg, VB 550mg, heme 50mg, 1,2-naphthoquinones 50mg, water surplus.
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Cited By (4)

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CN107929998A (en) * 2017-10-13 2018-04-20 浙江大学 A kind of charcoal preparation for mediating Polychlorinated biphenyls anaerobic reductive dechlorination
CN107929997A (en) * 2017-10-13 2018-04-20 浙江大学 A kind of application of rape straw charcoal in Polychlorinated biphenyls anaerobic reductive dechlorination is mediated
CN110951492A (en) * 2019-11-19 2020-04-03 西北农林科技大学 Preparation method and application of soil remediation medicament humin based on modified peat
CN112624330A (en) * 2020-12-30 2021-04-09 西安建筑科技大学 Method for promoting anaerobic degradation of phenolic wastewater by adding biochar

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