CN107926702A - A kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method - Google Patents
A kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method Download PDFInfo
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- CN107926702A CN107926702A CN201711227923.0A CN201711227923A CN107926702A CN 107926702 A CN107926702 A CN 107926702A CN 201711227923 A CN201711227923 A CN 201711227923A CN 107926702 A CN107926702 A CN 107926702A
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- dendrobium candidum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
Abstract
The present invention relates to dendrobium candidum tissue culture technique field, is specially a kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method.Main technical schemes are as follows：Take dendrobium candidum appearance just to switch to the capsule of brown, carry out sterilization processing, it is spare for explant to obtain sterile dendrobium candidum capsule；Disinfection explant is inoculated on solid medium, controlled at 18 22 DEG C, is cultivated 30 days, capsule starts to sprout greening；Dendrobium candidum protocorm fast breeding is carried out by fluid nutrient medium is added in dendrobium candidum protocorm；Make dendrobium candidum protocorm quick proliferation method with plant tissue culture technique the present invention provides one kind, substituting dendrobium candidum medicinal material for dendrobium candidum protocorm large-scale industrial production provides approach.
Description
Technical field
The invention belongs to dendrobium candidum tissue culture technique field, and in particular to a kind of dendrobium candidum protocorm fast breeding
Solid-liquid mixed culturing method.
Background technology
Dendrobium candidum is a kind of traditional rare traditional Chinese medicine (Dendrobium officinale Kimura et Migo),
Also known as：Ribbed hedyotis herb, Yunnan iron sheet.Belong to micro- specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family.Stem is upright, cylinder, 9-35 lis long
Rice, 2-4 millimeters thick, sepal and petal are in yellow green, and near similar, long round shape lanceolar, is about 1.8cm, wide 4-5mm, florescence 3-6
Month.It is distributed mainly on the ground such as micro- middle Guoan, Zhejiang, Fujian, Yunnan.Its stem is used as medicine, sweet in flavor, cold nature, belongs to the benefit in tonic
Cloudy medicine, reinforcing stomach reg fluid, nourishing Yin and clearing heat, the strong waist of improving eyesight.Its pharmacological action, which is mainly shown as, promotes glandular secretion and internal organs movement, tool
There is hypoglycemic effect.Dendrobium candidum particle (TPSH) can promote the phagocytic function of tumor animal macrophage, strengthen T lymphocytes
Propagation, the activity of differentiation and NK cells, and the serum hemolysin value of tumor animal can be significantly improved, TPSH is either to non-spy
Specific immunological function, or specific cellular immunity, humoral immune function, improve effect.But traditional use
Prescription formula is all directly to gather wild dendrobium candidum, and due to dendrobium candidum, breeding potential is extremely low under field conditions (factors), although its into
Containing the seed of up to ten thousand, upper 100,000 in ripe each pod, but because seed it is too tiny, without enough nutrition into
Divide and supply its germination and growth, so in contrast, breeding coefficient is very low under dendrobium candidum natural conditions, along with Growth of Dendrobium candidum
Cycle is longer, generally requires the growth cycle of 2 to three years and can just fetch medication.Data show the market demand of dendrobium candidum
Sustainable growth year after year is measured, for excessively picking and destruction causes dendrobium officinale endangered for wild resource.
In conclusion dendrobium candidum has very big medical value.But due to breeding coefficient under dendrobium candidum natural conditions
It is very low, along with the Growth of Dendrobium candidum cycle is longer and excessively picking and destruction causes wild iron sheet stone for wild resource
Dry measure used in former times is endangered.These drawbacks make dendrobium candidum industrialization to considerable restraint, it is impossible to meet the growing market demand.
The content of the invention
In order to solve the problems, such as dendrobium candidum, supply falls short of demand and alleviates environmental pressure, and the present invention cultivates iron using plant tissue
Skin stem of noble dendrobium protocorm, making its large-scale industrial production, concrete technical scheme is as follows to substitute dendrobium candidum medicinal material：A kind of iron sheet
Stem of noble dendrobium protocorm fast breeding solid-liquid mixed culturing method, has just switched to the capsule of brown as explant using dendrobium candidum appearance, Gu
Cultivated to obtain protocorm on body culture medium, then protocorm is put into fluid nutrient medium and carries out fast breeding；
Comprise the following steps that：
(1) disinfection of explant
Explant is cultivated and obtained as explant by the capsule for just having switched to brown using dendrobium candidum appearance on solid medium
Protocorm, then protocorm is put into fluid nutrient medium and carries out fast breeding；
Comprise the following steps that：
(1) disinfection of explant
Take dendrobium candidum appearance just to switch to the capsule of brown, carry out sterilization processing, be outer using sterile dendrobium candidum capsule
Implant is spare；
The aseptic process step is：Dendrobium candidum capsule 3min is soaked with cleaning powder, then pulls out and is rushed with tap water
Wash clean, aseptically with 70vt% alcohol surface sterilization 1.5min, sterile water wash 1 minute, then the secondary chlorine with 2wt%
Acid sodium solution soaks 10-15min, finally with sterile water wash 4-5 times, cleans 1min every time, sterilizing filter paper is used after flushing
Suck the moisture on dendrobium candidum capsule surface；
(2) dendrobium candidum protocorm solid medium culture
Explant obtained by step (1) is inoculated on solid medium, controlled at 18-22 DEG C, is cultivated 30 days, capsule
Fruit starts to sprout greening, is dendrobium candidum protocorm；
(3) dendrobium candidum protocorm liquid proliferated culture medium culture
Fast breeding is carried out by fluid nutrient medium is added in the dendrobium candidum protocorm obtained by step (2).
Further, the solid medium includes following component with every liter of metering：The solid medium is with every
Rising metering includes following component：1896mg KNO3、7.5mg MnSO4·H2O、125mg CaCl2·2H2O、28mg
Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg(NH4)2SO4、2mg ZnSO4·
7H2O, 0.2mg methyl α-naphthyl acetates, 22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·2H2O, 3.8mg indoles -3- second
Acid, 3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、1.3mg KI、310mg Vc、360mg MgSO4·
7H2O、1.85mg VB6, 288.56mg inositols, 5mg-6mg heteroauxins, 0.6mg-1.2mg 6- benzyls aminoadenine, 25g-
35g sucrose, 6g-7.5g agar, 0.5mg-1.2mg lactoalbumin hydrolysates, 3g-5g activated carbons, surplus are water.
Further, the solid medium includes following component with every liter of metering：1896mg KNO3、7.5mg
MnSO4·H2O、125mg CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg
CuSO4·5H2O、101mg (NH4)2SO4、2mg ZnSO4·7H2O, 0.2mg methyl α-naphthyl acetates, 22.8mg FeSO4·7H2O、14mg
VB1、0.4mg NaMoO4·2H2O, 3.8mg indole-3-acetic acids, 3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·
H2O、1.3mg KI、310mg Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 5mg heteroauxins,
0.6mg 6- benzyls aminoadenine, 25g sucrose, 6g agar, 0.5mg lactoalbumin hydrolysates, 3g activated carbons, surplus are water.
Further, the fluid nutrient medium includes following component with every liter of metering：1896mg KNO3、7.5mg
MnSO4·H2O、125mg CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg
CuSO4·5H2O、101mg(NH4)2SO4、2mg ZnSO4·7H2O、22.8mg FeSO4·7H2O、14mg VB1、0.4mg
NaMoO4·2H2O、3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、310mg Vc、360mg MgSO4·
7H2O、1.85mg VB6, 288.56mg inositols, 0.6mg-1.2mg 6-BA, 0.2mg-0.6mg NAA, 25g-35g sucrose,
0.5mg-1.2mg lactoalbumin hydrolysates, 10g-30g banana purees, 15g-30g mashed potatoess, surplus are water.
Further, the fluid nutrient medium includes following component with every liter of metering：1896mg KNO3、7.5mg
MnSO4·H2O、125mg CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg
CuSO4·5H2O、101mg(NH4)2SO4、2mg ZnSO4·7H2O、22.8mg FeSO4·7H2O、14mg VB1、0.4mg
NaMoO4·2H2O、3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、310mg Vc、360mg MgSO4·
7H2O、1.85mg VB6, 288.56mg inositols, 0.6mg 6-BA, 0.2mg NAA, 25g sucrose, 0.5mg lactoalbumin hydrolysates, 10g
Banana puree, 15g mashed potatoess, surplus are water.
The lactoalbumin hydrolysate is the conventional reagent of this area, commercially available；The murphy juice is peeled by potato squeezes the juice
Obtain, banana puree is smashed to pieces to obtain for peeling banana.
Protocorm (Protocorm) is that proembryo, which is sprouted, to be expanded in Seeds of Dendrobium Candidum germination process, dashes forward and is formed after breaking in the seed coat
Light yellow roundlet taper embryonal connective tissue.The explant blade tip of dendrobium candidum, stem apex, base point etc. can also form class by induction
It is similar to the embryonal connective tissue of protocorm.Protocorm can further bud into pseudobulb, then send out roots and leaf, formation are complete
Plant.But protocorm forms ripe plant and is limited by several factors, and required time is also long.The protocorm Stem nematode of dendrobium candidum
Quickly, self is mitogenetic vigorous, and vigor is lasting, is a kind of preferably dendrobium candidum medicinal material substitute.
Therefore the dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method of the present invention not only can be simple and efficient
Dendrobium candidum protocorm is obtained, and solves in the past that simple solid medium Protocorm Multiplication speed is slow, switching is numerous and diverse time-consuming
The problems such as laborious efficiency is low, make dendrobium candidum protocorm fast breeding the present invention provides one kind with plant tissue culture technique
Method, for dendrobium candidum protocorm can large-scale industrial production substitute dendrobium candidum medicinal material provide optimal path.
Beneficial effects of the present invention are as follows：
(1) present invention is mixed using dendrobium officinale capsule as explant using solid, liquid specific to the present invention
Base, a step obtain dendrobium candidum protocorm, and yield reaches 100%, and growth rate 1.0g/Ld, 30d can grow in good condition
The micro- yellow protocorm of light green color band point, can constantly breed after being placed in fluid nutrient medium, and solve transfer numerous and diverse, expense
When, the problem such as laborious efficiency is low；
(2) dendrobium candidum protocorm fast breeding can at utmost be realized using the method for the invention, in operation without mistake
In the case of by mistake, it can monthly breed 20 times, solve the problems, such as that those skilled in the art's growth rate urgently to be resolved hurrily is slow；
(3) cultural method provided by the invention has that simple, Growth of Dendrobium candidum state is good, the time required to Protocorm Multiplication
Short, the features such as speed is fast, sustainable growth, easy to mass liquid culture dendrobium candidum protocorm, solves dendrobium candidum medicine
The problem of with resource scarcity.
Brief description of the drawings
Fig. 1 is the protocorm figure that dendrobium candidum capsule explant derives；
Fig. 2 is primary growth situation map of the dendrobium candidum protocorm in solid medium；
Fig. 3 is that dendrobium candidum protocorm adds the fast breeding situation map after fluid nutrient medium.
Embodiment
With reference to specific embodiment, the invention will be further described.Following embodiments are descriptive, are not limits
Qualitatively, it is impossible to which protection scope of the present invention is limited with this.The chemical reagent and instrument that the present invention is applied are such as without special
Illustrate, can be bought from commercial channel.
Embodiment 1
Dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method, comprises the following steps that：
(1) disinfection of explant
Take dendrobium candidum appearance just to switch to the capsule of brown, carry out sterilization processing, obtaining sterile dendrobium candidum capsule is
Explant is spare：
The aseptic process step is：Dendrobium candidum capsule 3min is soaked with cleaning powder, then pulls out and is rushed with tap water
Wash clean, aseptically with 70vt% alcohol surface sterilization 1.5min, sterile water wash 1 minute, then the secondary chlorine with 2wt%
Acid sodium solution soaks 10min, finally with sterile water wash 4 times, cleans 1min every time, iron is sucked with sterilizing filter paper after flushing
The moisture on skin stem of noble dendrobium capsule surface；
(2) dendrobium candidum protocorm solid medium culture
Disinfection explant obtained by step (1) is inoculated on solid medium, controlled at 18 DEG C, is cultivated 30 days,
Capsule starts to sprout greening；The formula of the solid medium (1L) is as follows：1896mg KNO3、7.5mg MnSO4·H2O、
125mg CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、
101mg(NH4)2SO4、2mg ZnSO4·7H2O、0.2mg NAA、22.8mg FeSO4·7H2O、14mg VB1、0.4mg
NaMoO4·2H2O、3.8mg IAA、3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、1.3mg KI、310mg
Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 5mg IBA, 0.6mg 6-BA, 25g sucrose, 6g fine jades
Fat, 0.5mg lactoalbumin hydrolysates, the water of 3g activated carbons and surplus.
(3) protocorm liquid proliferated culture medium culture
Fluid nutrient medium progress dendrobium candidum protocorm will be added in dendrobium candidum protocorm obtained by step (2) quickly to increase
Grow；The formula of the fluid nutrient medium (1L) is as follows：1896mg KNO3、7.5mg MnSO4·H2O、125mg CaCl2·2H2O、
28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg(NH4)2SO4、2mg
ZnSO4·7H2O、22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·2H2O、3.5mg H3BO3, 1.0mg cigarettes
Acid, 120mg NaH2PO4·H2O、310mg Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 0.6mg
6-BA, 0.2mg NAA, 25g sucrose, 0.5mg lactoalbumin hydrolysates, 10g banana purees, the water of 15g mashed potatoess and surplus.
Embodiment 2
Dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method, comprises the following steps that：
(1) disinfection of explant
Take dendrobium candidum appearance just to switch to the capsule of brown, carry out sterilization processing, obtaining sterile dendrobium candidum capsule is
Explant is spare：
The aseptic process step is：Dendrobium candidum capsule 3min is soaked with cleaning powder, then pulls out and is rushed with tap water
Wash clean, aseptically with 70vt% alcohol surface sterilization 1.5min, sterile water wash 1 minute, then the secondary chlorine with 2wt%
Acid sodium solution soaks 10min, finally with sterile water wash 5 times, cleans 1min every time, iron is sucked with sterilizing filter paper after flushing
The moisture on skin stem of noble dendrobium capsule surface；
(2) dendrobium candidum protocorm solid medium culture
Disinfection explant obtained by step (1) is inoculated on solid medium, controlled at 18 DEG C, is cultivated 30 days,
Capsule starts to sprout greening；The formula of the solid medium (1L) is as follows：1896mg KNO3、7.5mg MnSO4·H2O、
125mg CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、
101mg(NH4)2SO4、2mg ZnSO4·7H2O、0.6mg NAA、22.8mg FeSO4·7H2O、14mg VB1、0.4mg
NaMoO4·2H2O、3.8mg IAA、3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、1.3mg KI、310mg
Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 6mg IBA, 1.2mg 6-BA, 35g sucrose, 7.5g fine jades
Fat, 1.2mg lactoalbumin hydrolysates, 5g activated carbons, the water of surplus.
(3) protocorm liquid proliferated culture medium culture
Fluid nutrient medium progress dendrobium candidum protocorm will be added in dendrobium candidum protocorm obtained by step (2) quickly to increase
Grow；The formula of the fluid nutrient medium (1L) is as follows：1896mg KNO3、7.5mg MnSO4·H2O、125mg CaCl2·2H2O、
28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg(NH4)2SO4、2mg
ZnSO4·7H2O、22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·2H2O、3.5mg H3BO3, 1.0mg cigarettes
Acid, 120mg NaH2PO4·H2O、310mg Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 1.2mg
6-BA, 0.6mg NAA, 35g sucrose, 1.2mg lactoalbumin hydrolysates, 30g banana purees, 30g mashed potatoess, surplus are water.
Cultivate obtained dendrobium candidum protocorm and see attached drawing 1-3, it can be seen that：Dendrobium candidum protocorm is made using the present invention
Proliferation rate it is higher, growth coefficient can reach 20 times, and protocorm shows faint yellow, and full grains are mellow and full, on surface with fine hair
Shape thing indicates that dendrobium candidum protocorm rapid multiplication long status is good, and multiplication rate is fast, is that dendrobium candidum protocorm can be extensive
Industrialized production substitutes dendrobium candidum medicinal material and provides new method.
Claims (5)
1. a kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method, it is characterised in that firm with dendrobium candidum appearance
The capsule for switching to brown is explant, and explant is cultivated on solid medium and obtains protocorm, then protocorm is put into liquid
Body culture medium carries out fast breeding；
Comprise the following steps that：
(1) disinfection of explant
Take dendrobium candidum appearance just to switch to the capsule of brown, sterilization processing is carried out, using sterile dendrobium candidum capsule as explant
It is spare；
The aseptic process step is：Dendrobium candidum capsule 3min is soaked with cleaning powder, then pulls out to be rinsed with tap water and does
Only, aseptically with 70vt% alcohol surface sterilization 1.5min, sterile water wash 1 minute, then the sodium hypochlorite with 2wt%
Solution soaks 10-15min, finally with sterile water wash 4-5 times, cleans 1min every time, is sucked after flushing with sterilizing filter paper
The moisture on dendrobium candidum capsule surface；
(2) dendrobium candidum protocorm solid medium culture
Explant obtained by step (1) is inoculated on solid medium, controlled at 18-22 DEG C, is cultivated 30 days, capsule is opened
Begin to sprout greening, be dendrobium candidum protocorm；
(3) dendrobium candidum protocorm liquid proliferated culture medium culture
Fast breeding is carried out by fluid nutrient medium is added in the dendrobium candidum protocorm obtained by step (2).
2. a kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method according to claim 1, its feature exist
In the solid medium includes following component with every liter of metering：1896mg KNO3、7.5mg MnSO4·H2O、125mg
CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg
(NH4)2SO4、2mg ZnSO4·7H2O, 0.2mg methyl α-naphthyl acetates, 22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·
2H2O, 3.8mg indole-3-acetic acids, 3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、1.3mg KI、310mg
Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 5mg-6mg heteroauxins, 0.6mg-1.2mg 6- benzyls
Aminoadenine, 25g-35g sucrose, 6g-7.5g agar, 0.5mg-1.2mg lactoalbumin hydrolysates, 3g-5g activated carbons, surplus are
Water.
3. a kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method according to claim 1, its feature exist
In the solid medium includes following component with every liter of metering：1896mg KNO3、7.5mg MnSO4·H2O、125mg
CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg
(NH4)2SO4、2mg ZnSO4·7H2O, 0.2mg methyl α-naphthyl acetates, 22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·
2H2O, 3.8mg indole-3-acetic acids, 3.5mg H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、1.3mg KI、310mg
Vc、360mg MgSO4·7H2O、1.85mg VB6, 288.56mg inositols, 5mg heteroauxins, 0.6mg 6- benzyls aminoadenine,
25g sucrose, 6g agar, 0.5mg lactoalbumin hydrolysates, 3g activated carbons, surplus are water.
4. a kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method according to claim 1, its feature exist
In the fluid nutrient medium includes following component with every liter of metering：1896mg KNO3、7.5mg MnSO4·H2O、125mg
CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg
(NH4)2SO4、2mg ZnSO4·7H2O、22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·2H2O、3.5mg
H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、310mg Vc、360mg MgSO4·7H2O、1.85mg VB6、
288.56mg inositols, 0.6mg-1.2mg 6-BA, 0.2mg-0.6mg NAA, 25g-35g sucrose, the newborn egg of 0.5mg-1.2mg hydrolysis
In vain, 10g-30g banana purees, 15g-30g mashed potatoess, surplus are water.
5. a kind of dendrobium candidum protocorm fast breeding solid-liquid mixed culturing method according to claim 1, its feature exist
In：The fluid nutrient medium includes following component with every liter of metering：1896mg KNO3、7.5mg MnSO4·H2O、125mg
CaCl2·2H2O、28mg Na2EDTA·2H2O、0.187mg CoCl2·6H2O、0.18mg CuSO4·5H2O、101mg
(NH4)2SO4、2mg ZnSO4·7H2O、22.8mg FeSO4·7H2O、14mg VB1、0.4mg NaMoO4·2H2O、3.5mg
H3BO3, 1.0mg nicotinic acid, 120mg NaH2PO4·H2O、310mg Vc、360mg MgSO4·7H2O、1.85mg VB6、
288.56mg inositols, 0.6mg 6-BA, 0.2mg NAA, 25g sucrose, 0.5mg lactoalbumin hydrolysates, 10g banana purees, 15g potatoes
Mud, surplus are water.
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| CN113331053A (en) * | 2021-05-26 | 2021-09-03 | 海南大学 | Separation breeding method of dendrobium pure-color flowers |
| CN114831027A (en) * | 2022-05-25 | 2022-08-02 | 广西壮族自治区中国科学院广西植物研究所 | Dendrobium devonianum tissue culture rapid propagation culture medium combination and application thereof in dendrobium devonianum seedling cultivation |
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| CN113331053B (en) * | 2021-05-26 | 2022-05-24 | 海南大学 | Separation breeding method of dendrobium pure-color flowers |
| CN114831027A (en) * | 2022-05-25 | 2022-08-02 | 广西壮族自治区中国科学院广西植物研究所 | Dendrobium devonianum tissue culture rapid propagation culture medium combination and application thereof in dendrobium devonianum seedling cultivation |
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