CN107904149A - A kind of microcarrier reclaimer and method - Google Patents
A kind of microcarrier reclaimer and method Download PDFInfo
- Publication number
- CN107904149A CN107904149A CN201711211146.0A CN201711211146A CN107904149A CN 107904149 A CN107904149 A CN 107904149A CN 201711211146 A CN201711211146 A CN 201711211146A CN 107904149 A CN107904149 A CN 107904149A
- Authority
- CN
- China
- Prior art keywords
- cleaning solution
- filter
- microcarrier
- solution bucket
- retracting device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of microcarrier reclaimer, retracting device, filter, cleaning solution bucket, pump;The filter outlet connects retracting device bottom inlet, and filter inlet connection cleaning solution bucket, cleaning solution bucket connection retracting device upper outlet, pumps and be arranged between filter and cleaning solution bucket;The retracting device is sealing structure, and centre sets filter core, and the aperture of filter core is less than microcarrier diameter.The present invention also provides a kind of recovery method using the reclaimer, beneficial effects of the present invention:The method of the present invention is simple, and recovery time is short, and organic efficiency is high, reuses often, effectively reduces production cost.
Description
Technical field
Suspend to cultivate the present invention relates to one kind and use carrier recovering apparatus and recovery method, more particularly to a kind of micro- load
Body reclaimer and recovery method.
Background technology
Microcarrier is used for cell suspension cultures, and since microcarrier price is very high, the recycling and recycling of microcarrier are just
An important link into production, efficient retracting device invention, in production very important effect.
Existing microcarrier recycling carries out all in container, and recovery process is cumbersome, and recovery time is very long, organic efficiency
It is low.
The content of the invention
The technical problems to be solved by the invention are the provision of that a kind of recovery process is simple, and recovery time is short, and recycling is high
The retracting device of effect and the recovery method realized using the equipment.
The present invention is to solve above-mentioned technical problem by the following technical programs:The present invention include retracting device, filter,
Cleaning solution bucket, pump;The filter outlet connects retracting device bottom inlet, filter inlet connection cleaning solution bucket, cleaning solution
Bucket connection retracting device upper outlet, pump are arranged between filter and cleaning solution bucket;
The retracting device is sealing structure, and centre sets filter core, and the aperture of filter core is less than microcarrier diameter.
Preferably, the retracting device includes end socket, shell, filter core, base, and top and the end socket sealing of the shell connect
Connecing, lower part is tightly connected with base, and filter core is arranged on housing center portion, and the top of the filter core is tightly connected with end socket, under
Portion is tightly connected with base.
Preferably, the end socket middle part sets microcarrier to import and export, and the first valve is set on the pipeline of inlet and outlet
Door.
Preferably, the shell is hollow cylinder, and upper side opens up a rinse liquid outlet, and in cleaning solution
Second valve is set on outlet conduit.
Preferably, the filter core is cylinder, including micropore portion, solid plate portion, filter screen, micropore filter plate, micropore portion and reality
Plate portion is integrally formed, and filter screen is fixed on micropore portion inner surface, and micropore filter plate is spaced for two, one of micropore filter plate
Installed in solid plate portion end, another is installed on micropore portion and solid plate portion intersection.
Preferably, the micropore portion is the stainless steel plate that aperture is 10 millimeters, and solid plate portion is solid stainless steel plate, filtering
Net is 75 microns of stainless (steel) wire, and micropore filter plate is 75 microns of filter plate.
Preferably, the base includes three support legs, bottom plates, and support leg is uniformly installed on bottom base plate, bottom plate middle part
Cleaning solution import is set, and the 3rd valve is set on cleaning solution inlet pipeline.
Preferably, the filter is 1 micron filter.
The present invention also provides a kind of microcarrier recovery method, the step of recovery method, is as follows:
(1) microcarrier after is added in 0.05-0.2mol/L sodium hydroxide solutions, opens the first valve, will
Microcarrier is poured into filter core from microcarrier inlet and outlet, closes the first valve;
(2) prepare suitable 0.05-0.2mol/L sodium hydroxide solutions in cleaning solution bucket, and open the second valve, the
Three valves, open pump, allow sodium hydroxide solution to enter filter element cleaning microcarrier by 1 micron filter, and at the second valve
Cleaning solution bucket is back to, this process wash cycles 30 minutes, then the sodium hydroxide in drained retracting device and cleaning solution bucket is molten
Liquid;
(3) physiological saline (EDTA for containing 0.02%) of suitable PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to
Cleaning solution in retracting device and cleaning solution bucket is completely exhausted out by step (2) after wash cycles 10-15 minutes;
(4) suitable 0.04%-0.07% trypsin solutions are prepared, and are heated to 37 DEG C, are poured into cleaning solution bucket, according to step
Cleaning solution in retracting device and cleaning solution bucket is completely exhausted out by rapid two wash cycles after 30-40 minutes;
(5) new 0.05-0.2mol/L sodium hydroxide solutions are prepared, are poured into cleaning solution bucket, according to step (2) circulation cleaning
After washing 15-20 minutes, the cleaning solution in retracting device and cleaning solution bucket is completely exhausted out;
(6) physiological saline of new PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to step (2) wash cycles 5-
After ten minutes, the cleaning solution in retracting device and cleaning solution bucket is completely exhausted out, the cleaning operation of repeat step six, until PH is
Untill 7.2-7.5;
(7) microcarrier recycling is got up with clean-cloth filtering after cleaning, and places 80 DEG C of baking ovens, drying.
The present invention has the following advantages compared with prior art:Apparatus of the present invention are simple, and filter screen is 75 microns of stainless steel
Net, micropore filter plate are 75 microns of filter plate, can effectively realize the filtering of microcarrier, and the method for the present invention is simple, and recovery time is short,
Organic efficiency is high, reuses often, effectively reduces production cost.
Brief description of the drawings
Fig. 1 is a kind of structure diagram of microcarrier reclaimer of the present invention;
Fig. 2 is the structure diagram of retracting device in Fig. 1;
Fig. 3 is the structure diagram of filter core in Fig. 2;
Fig. 4 is Fig. 3 filter core outer layer expanded schematic diagrams.
Retracting device 1, end socket 11, microcarrier import and export the 111, first valve 112, shell 12, rinse liquid outlet 121, the
Two valves 122, filter core 13, micropore portion 131, solid plate portion 132, filter screen 133, micropore filter plate 134, base 14, support leg 141,
Bottom plate 142, rinse liquid outlet 143,144 filter 2 of the 3rd valve, cleaning solution bucket 3, pump 4
Embodiment
Elaborate below to the embodiment of the present invention, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
As shown in Figure 1, a kind of microcarrier reclaimer of the present embodiment, including retracting device 1, filter 2, cleaning solution bucket 3,
Pump 4;The outlet of filter 2 connection 1 bottom inlet of retracting device, 2 import of filter connection cleaning solution bucket 3, cleaning solution bucket 3 connects
1 upper outlet of receiving apparatus is taken back, pump 4 is arranged between filter 2 and cleaning solution bucket 3;The retracting device 1 is sealing structure,
Centre sets filter core 13, and the aperture of filter core 13 is less than microcarrier diameter.
As shown in Fig. 2, the retracting device 1 include end socket 11, shell 12, filter core 13, base 14, the shell 12 it is upper
Portion is tightly connected with end socket 11, and lower part is tightly connected with base 14, and filter core 13 is arranged on 12 central part of shell, and the filter core 13
Top be tightly connected with end socket 11, lower part is tightly connected with base 14.
11 middle part of end socket sets microcarrier to import and export 111, and the first valve is set on the pipeline of inlet and outlet
112.The shell 12 is hollow cylinder, and upper side opens up a rinse liquid outlet 121, and in rinse liquid outlet pipe
Second valve 122 is set on road.The base 14 includes three support legs 141, bottom plate 142, and support leg is uniformly installed on bottom plate
Bottom, bottom plate middle part sets cleaning solution import 143, and the 3rd valve 144 is set on cleaning solution inlet pipeline.
As shown in Figure 3-4, the filter core 13 is cylinder, including micropore portion 131, solid plate portion 132, filter screen 133 are (in figure
Be not shown), micropore filter plate 134, micropore portion 131 is integrally formed with solid plate portion 132, and filter screen 133 is fixed on table in micropore portion 131
Face, micropore filter plate 134 are spaced for two, and one of micropore filter plate 134 is installed on 132 end of solid plate portion, another peace
Mounted in micropore portion 131 and 132 intersection of solid plate portion.The micropore portion 131 is the stainless steel plate that aperture is 10 millimeters, solid plate portion
132 be solid stainless steel plate, and filter screen 133 is 75 microns of stainless (steel) wire, and micropore filter plate 134 is 75 microns of filter plate.
The filter is 1 micron filter.
The present invention also provides a kind of microcarrier recovery method, the step of recovery method embodiment, is as follows:
Embodiment one:
(1) microcarrier after is added in 0.1mol/L sodium hydroxide solutions, opens the first valve 112, will be micro-
Carrier is poured into filter core from microcarrier inlet and outlet, closes the first valve 112;
(2) suitable 0.1mol/L sodium hydroxide solutions are prepared in cleaning solution bucket 3, and open the second valve the 122, the 3rd
Valve 144, opens pump, allows sodium hydroxide solution to enter filter element cleaning microcarrier by 1 micron filter, and from the second valve
Cleaning solution bucket 3 is back at 122, will wash out dirt by this process returns to cleaning solution bucket 3, and retains and 1 micron membrane filter, guarantee
The sodium hydroxide solution being recycled into is clean, this process wash cycles 30 minutes, then drained retracting device 1 and cleaning solution bucket 3
Interior sodium hydroxide solution;
(3) physiological saline (EDTA for containing 0.02%) of suitable PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to
After ten minutes, the cleaning solution in retracting device 1 and cleaning solution bucket 3 is completely exhausted out for step (2) wash cycles;
(4) suitable 0.05% trypsin solution is prepared, and is heated to 37 DEG C, pours into cleaning solution bucket, is followed according to step 2
After ring cleans 30 minutes, the cleaning solution in retracting device 1 and cleaning solution bucket 3 is completely exhausted out;
(5) new 0.1mol/L sodium hydroxide solutions are prepared, are poured into cleaning solution bucket 3, according to step (2) wash cycles
After 15-20 minutes, the cleaning solution in retracting device 1 and cleaning solution bucket 3 is completely exhausted out;
(6) physiological saline of new PBS or 0.9% is prepared, is poured into cleaning solution bucket 3, according to step (2) wash cycles 5
After minute, the cleaning solution in 3 barrels of retracting device 1 and cleaning solution is completely exhausted out, the cleaning operation of repeat step (6), until PH
Untill 7.4;
(7) microcarrier recycling is got up with clean-cloth filtering after cleaning, and places 80 DEG C of baking ovens, drying.
Embodiment two:
(1) microcarrier after is added in 0.05mol/L sodium hydroxide solutions, opens the first valve 112, will be micro-
Carrier is poured into filter core from microcarrier inlet and outlet, closes the first valve 112;
(2) suitable 0.05mol/L sodium hydroxide solutions are prepared in cleaning solution bucket, and open the second valve the 122, the 3rd
Valve 144, opens pump, allows sodium hydroxide solution to enter filter element cleaning microcarrier by 1 micron filter, and from the second valve
Cleaning solution bucket 3 is back at 122, will wash out dirt by this process returns to cleaning solution bucket 3, and retains and 1 micron membrane filter, guarantee
The sodium hydroxide solution being recycled into is clean, this process wash cycles 30 minutes, then drained retracting device 1 and cleaning solution bucket 3
Interior sodium hydroxide solution;
(3) physiological saline (EDTA for containing 0.02%) of suitable PBS or 0.9% is prepared, pours into cleaning solution bucket 3, presses
After step (2) wash cycles 15 minutes, the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out;
(4) suitable 0.04% trypsin solution is prepared, and is heated to 37 DEG C, pours into cleaning solution bucket, is followed according to step 2
Ring cleans after forty minutes, and the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out;
(5) new 0.05mol/L sodium hydroxide solutions are prepared, are poured into cleaning solution bucket, according to step (2) wash cycles 20
After minute, the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out;
(6) physiological saline of new PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to step (2) wash cycles 10
After minute, the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out, the cleaning operation of repeat step (6), until PH
Untill 7.4;
(7) microcarrier recycling is got up with clean-cloth filtering after cleaning, and places 80 DEG C of baking ovens, drying.
Embodiment three:
(1) microcarrier after is added in 0.2mol/L sodium hydroxide solutions, opens the first valve 112, will be micro-
Carrier is poured into filter core from microcarrier inlet and outlet, closes the first valve 112;
(2) suitable 0.2mol/L sodium hydroxide solutions are prepared in cleaning solution bucket, and open the second valve the 122, the 3rd
Valve 144, opens pump, allows sodium hydroxide solution to enter filter element cleaning microcarrier by 1 micron filter, and from the second valve
Cleaning solution bucket is back at 122, will wash out dirt by this process returns to cleaning solution bucket, and retention and 1 micron membrane filter, ensures to follow
The sodium hydroxide solution that ring enters is clean, this process wash cycles 30 minutes, then in drained reclaimer 1 and cleaning solution bucket 3
Sodium hydroxide solution;
(3) physiological saline (EDTA for containing 0.02%) of suitable PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to
Cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out by step (2) wash cycles after 15 minutes;
(4) suitable 0.07% trypsin solution is prepared, and is heated to 37 DEG C, pours into cleaning solution bucket, is followed according to step 2
After ring cleans 35 minutes, the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out;
(5) new 0.2mol/L sodium hydroxide solutions are prepared, are poured into cleaning solution bucket 3, according to step (2) wash cycles 15
After minute, the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out;
(6) physiological saline of new PBS or 0.9% is prepared, is poured into cleaning solution bucket 3, according to step (2) wash cycles 7
After minute, the cleaning solution in reclaimer 1 and cleaning solution bucket 3 is completely exhausted out, the cleaning operation of repeat step (6), until PH
Untill 7.6;
(7) microcarrier recycling is got up with clean-cloth filtering after cleaning, and places 80 DEG C of baking ovens, drying.
In order to verify the effect of practical operation, actual production is carried out, for micro- after the not synantigen production of inequality
Carrier recovery is operated.
Operation one:The culture that suspends produces the microcarrier processing after 5L rabies antigens:According to the operation instruction of retracting device,
Microcarrier after use is recycled, from starting to drying recycling terminate, amount to 135 minutes
Operation two:The culture that suspends produces the microcarrier processing after 30L rabies antigens:Said according to the use of retracting device
It is bright, the microcarrier after use is recycled, from starting to drying recycling terminate, amount to 140 minutes.
Operation three:The culture that suspends produces the microcarrier processing after 5L transmissible gastroenteritis of swine antigens:According to retracting device
Operation instruction, the microcarrier after use is recycled, from starting to drying recycling terminate, amount to 135 minutes.
Operation four:The culture that suspends produces the microcarrier processing after 30L transmissible gastroenteritis of swine antigens:According to retracting device
Operation instruction, the microcarrier after use is recycled, from starting to drying recycling terminate, amount to 140 minutes.
In whole process, cleaning solution is prepared in advance, in use, be added directly into cleaning solution bucket, it is non-
Often convenient, the microcarrier character after recycling is good, and one people of whole operation process can be easily accomplished.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of microcarrier reclaimer, it is characterised in that including retracting device, filter, cleaning solution bucket, pump;The filtering
Device outlet connection retracting device bottom inlet, filter inlet connection cleaning solution bucket, cleaning solution bucket connection retracting device top go out
Mouthful, pump is arranged between filter and cleaning solution bucket;
The retracting device is sealing structure, and centre sets filter core, and the aperture of filter core is less than microcarrier diameter.
A kind of 2. microcarrier reclaimer according to claim 1, it is characterised in that the retracting device include end socket,
Shell, filter core, base, top and the end socket of the shell are tightly connected, and lower part is tightly connected with base, and filter core is arranged on shell
Central part, and the top of the filter core is tightly connected with end socket, lower part is tightly connected with base.
3. a kind of microcarrier reclaimer according to claim 2, it is characterised in that the end socket middle part sets micro-
Carrier is imported and exported, and the first valve is set on the pipeline of inlet and outlet.
4. a kind of microcarrier reclaimer according to claim 2, it is characterised in that the shell is hollow cylinder
Body, and upper side opens up a rinse liquid outlet, and the second valve is set on rinse liquid outlet pipeline.
A kind of 5. microcarrier reclaimer according to claim 2, it is characterised in that the filter core is cylinder, including
Micropore portion, solid plate portion, filter screen, micropore filter plate, micropore portion are integrally formed with solid plate portion, and filter screen is fixed on table in micropore portion
Face, micropore filter plate are spaced for two, and one of micropore filter plate is installed on solid plate portion end, another is installed on micropore portion
With solid plate portion intersection.
6. a kind of microcarrier reclaimer according to claim 5, it is characterised in that the micropore portion is that aperture is 10 millis
The stainless steel plate of rice, solid plate portion are solid stainless steel plate, and filter screen is 75 microns of stainless (steel) wire, and micropore filter plate is 75 microns
Filter plate.
7. a kind of microcarrier reclaimer according to claim 2, it is characterised in that the base includes three supports
Leg, bottom plate, support leg are uniformly installed on bottom base plate, cleaning solution import are set in the middle part of bottom plate, and on cleaning solution inlet pipeline
3rd valve is set.
8. a kind of microcarrier reclaimer according to claim 1, it is characterised in that the filter is 1 Mm filter
Device.
A kind of 9. recovery method using any microcarrier reclaimers of claim 1-8, it is characterised in that the recycling
The step of method, is as follows:
(1) microcarrier after is added in 0.05-0.2mol/L sodium hydroxide solutions, the first valve is opened, by micro- load
Body is poured into filter core from microcarrier inlet and outlet, closes the first valve;
(2) suitable 0.05-0.2mol/L sodium hydroxide solutions are prepared in cleaning solution bucket, and open the second valve, the 3rd valve
Door, opens pump, allows sodium hydroxide solution to enter filter element cleaning microcarrier by 1 micron filter, and flow back at the second valve
To cleaning solution bucket, this process wash cycles 30-40 minutes, then the sodium hydroxide in drained retracting device and cleaning solution bucket is molten
Liquid.
(3) physiological saline (EDTA for containing 0.02%) of suitable PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to step
(2) after wash cycles 10-15 minutes, the cleaning solution in retracting device and cleaning solution bucket is completely exhausted out;
(4) suitable 0.04%-0.07% trypsin solutions are prepared, and are heated to 37 DEG C, are poured into cleaning solution bucket, according to step
(2) after wash cycles 30-40 minutes, the cleaning solution in retracting device and cleaning solution bucket is completely exhausted out;
(5) new 0.05-0.2mol/L sodium hydroxide solutions are prepared, are poured into cleaning solution bucket, according to step (2) wash cycles
After 15-20 minutes, the cleaning solution in retracting device and cleaning solution bucket is completely exhausted out;
(6) physiological saline of new PBS or 0.9% is prepared, is poured into cleaning solution bucket, according to step (2) wash cycles 5-10 points
Zhong Hou, the cleaning solution in retracting device and cleaning solution bucket is completely exhausted out, repeat step
(6) cleaning operation, untill PH is 7.2-7.5;
(7) microcarrier recycling is got up with clean-cloth filtering after cleaning, and places 80 DEG C of baking ovens, drying.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711211146.0A CN107904149A (en) | 2017-11-28 | 2017-11-28 | A kind of microcarrier reclaimer and method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711211146.0A CN107904149A (en) | 2017-11-28 | 2017-11-28 | A kind of microcarrier reclaimer and method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107904149A true CN107904149A (en) | 2018-04-13 |
Family
ID=61849062
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711211146.0A Pending CN107904149A (en) | 2017-11-28 | 2017-11-28 | A kind of microcarrier reclaimer and method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107904149A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111847584A (en) * | 2020-08-18 | 2020-10-30 | 昆山三一环保科技有限公司 | Device and method for movably cleaning filter element of security filter on line |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5139953A (en) * | 1989-08-29 | 1992-08-18 | Snow Brand Milk Products Co., Ltd. | Rotary column reactor |
CN102586173A (en) * | 2012-03-20 | 2012-07-18 | 广东大华农动物保健品股份有限公司 | Recovery processing method for increasing reuse times of ultra-microcarrier |
CN206408211U (en) * | 2017-01-03 | 2017-08-15 | 北京首钢朗泽新能源科技有限公司 | A kind of regenerating unit of vulcanized sodium |
CN206500394U (en) * | 2016-08-03 | 2017-09-19 | 广州诺诚生物制品股份有限公司 | A kind of bioreactor chip carrier cleaning device |
CN206535839U (en) * | 2017-01-24 | 2017-10-03 | 杭州凡腾科技股份有限公司 | A kind of CIP on-line cleaning systems |
-
2017
- 2017-11-28 CN CN201711211146.0A patent/CN107904149A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5139953A (en) * | 1989-08-29 | 1992-08-18 | Snow Brand Milk Products Co., Ltd. | Rotary column reactor |
CN102586173A (en) * | 2012-03-20 | 2012-07-18 | 广东大华农动物保健品股份有限公司 | Recovery processing method for increasing reuse times of ultra-microcarrier |
CN206500394U (en) * | 2016-08-03 | 2017-09-19 | 广州诺诚生物制品股份有限公司 | A kind of bioreactor chip carrier cleaning device |
CN206408211U (en) * | 2017-01-03 | 2017-08-15 | 北京首钢朗泽新能源科技有限公司 | A kind of regenerating unit of vulcanized sodium |
CN206535839U (en) * | 2017-01-24 | 2017-10-03 | 杭州凡腾科技股份有限公司 | A kind of CIP on-line cleaning systems |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111847584A (en) * | 2020-08-18 | 2020-10-30 | 昆山三一环保科技有限公司 | Device and method for movably cleaning filter element of security filter on line |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104664311B (en) | Soy sauce return pipe equipment | |
CN107904149A (en) | A kind of microcarrier reclaimer and method | |
CN109554288A (en) | A kind of MSC is separately cultured device and its separation method | |
CN207950815U (en) | Magnetic-type filter | |
CN202893015U (en) | Cleaning device for fiber ball filter | |
CN208628014U (en) | A kind of nuclear magnetic tube automatic flushing device | |
CN207845641U (en) | A kind of horizontal Flat photobioreactor | |
CN213051794U (en) | Production of wisdom building circuit board is with soaking device | |
CN205386458U (en) | Frame drips in laboratory | |
CN211328413U (en) | Novel pumpkin food preparation is with filter | |
CN207457021U (en) | A kind of water-permeable brick water permeability simulation test device | |
CN210278683U (en) | Steam utilization device suitable for energy saving and consumption reduction of oil refinery | |
CN203220822U (en) | Biogas slurry filter | |
CN208877131U (en) | A kind of gynecological nursing is removable to rinse vehicle | |
CN207727071U (en) | A kind of ethyl alcohol remove device for nucleic acid extraction experiment | |
CN204183353U (en) | A kind of optical glass material block grinding table | |
CN205995872U (en) | A kind of stainless steel disc can washing apparatus | |
CN209174481U (en) | A kind of citric-acid washing working-procedure washing-water utilizing device | |
CN206356315U (en) | A kind of multiple-grooved ultrasonic cleaning equipment | |
CN212640113U (en) | Sewage circulation is applied mechanically processing apparatus | |
CN107551651A (en) | A kind of intelligently filters device | |
CN209006356U (en) | A kind of recycling vial cleaning device | |
CN104630128B (en) | A kind of method for regeneration treatment cell and Virus culture microcarrier | |
CN208583052U (en) | A kind of backwashing water processing unit | |
CN213518886U (en) | Device for preventing water leakage in human abdominal cavity imitation product processing |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180413 |