CN107897186B - Application of mycophenolic acid in inhibition of litchi downy blight - Google Patents
Application of mycophenolic acid in inhibition of litchi downy blight Download PDFInfo
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- CN107897186B CN107897186B CN201711160719.1A CN201711160719A CN107897186B CN 107897186 B CN107897186 B CN 107897186B CN 201711160719 A CN201711160719 A CN 201711160719A CN 107897186 B CN107897186 B CN 107897186B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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Abstract
The invention discloses application of mycophenolic acid in inhibition of litchi downy blight. According to the invention, the mycophenolic acid is researched for the first time and has important application in preventing and treating the peronophythora litchi, and particularly can inhibit the growth of peronophythora litchi hyphae and inhibit the germination of peronophythora litchi sporangia, so that the occurrence of peronophythora litchi is effectively inhibited. Moreover, mycophenolic acid can not completely kill pathogenic bacteria while inhibiting the growth of the pathogenic bacteria, which shows that the mycophenolic acid has low toxicity and is more environment-friendly. Therefore, the method has good application prospect in the aspect of preventing and treating the litchi frost blight.
Description
Technical Field
The invention relates to the technical field of plant diseases, and in particular relates to application of mycophenolic acid in inhibition of litchi downy blight.
Background
Litchi is a good fruit in Lingnan of China, is popular among China due to its beautiful color, fragrance and taste, and is called "fruit king". The litchi is an important raw material for developing food industry, the litchi industry plays a role in promoting the economic trade development in south China, and the litchi has high economic value in international and domestic markets. In production, because of being infected by some diseases, the quality and the yield of the litchi are seriously affected, wherein the litchi frost blight is the most prominent. The litchi frost blight is caused by Phytophthora litchi Fr (Lepidogrypus litchi) (Peronospora litchi)Peronophythora litchii) Infection by infectionThereby, the effect is achieved. The occurrence and prevalence of litchi frost blight are deeply influenced by environmental conditions, and are seriously damaged in warm and rainy spring and summer, which causes a great amount of flower falling, fruit cracking and rotten fruits. Investigation shows that litchi frost blight occurs in litchi producing areas all over the country, is seriously harmful and occupies an absolute position. The litchi gardens investigated in production areas such as Guangdong, Guangxi, Fujian and Sichuan occur. Peronophythora litchi (A. litchi.), (B. litchi.), (A. litchi.), (B. litchi)P. litchii) Belonging to the kingdom of algae (Chromista), Oomycota (Oomycota), Oomycota (Oomycotes), Peronospora (Peronosporas), Peronothoraceae (Peronothoraceae), and Phytophthora (Peronospora)Peronophythora). The life history of the phytophthora parasitica has two stages, namely, the asexual stage comprises sporangium peduncles and sporangium, the sporocyst peduncles are highly differentiated, are different in length and are similar to tree shapes, and the sporocyst peduncles belong to multi-stage limited growth. The sexual stage is oospore.
Peronophythora litchi overwintering in diseased leaves and diseased fruits by oospores or mycelia and becoming a primary infection source in the next year, sporangium can be generated and spread along with wind and rain, a germ tube can germinate when meeting water, or a germ tube can grow after the zoospores are released, so that the sporocysts invade leaves and fruits to cause diseases. The diseased leaves and fruits regenerate sporangia and are infected again, thereby completing the infection cycle. Therefore, the method for preventing and treating the peronophythora litchi mainly inhibits the growth of peronophythora litchi hyphae and the germination of sporangium.
At present, the main control measures of litchi frost blight are agricultural control and chemical control. Because the agricultural control operation is complex and takes effect slowly, chemical control with high control effect and faster effect is mostly adopted in the production area, but part of chemical pesticides have higher toxicity and greater harm to the environment, and a chemical inhibitor which has low toxicity and is environment-friendly and can effectively inhibit phytophthora litchi is absent.
Disclosure of Invention
The first purpose of the invention is to overcome the defects of the prior art and provide the application of mycophenolic acid in preventing/treating litchi downy blight.
The second purpose of the invention is to overcome the defects of the prior art and provide the application of mycophenolic acid in preparing the medicament for preventing/treating litchi downy blight.
The third purpose of the invention is to overcome the existing problemsThe deficiency of the prior art provides mycophenolic acid for preventing/treating phytophthora litchi: (Peronophythora litchii) The use of (1).
The fourth purpose of the invention is to overcome the defects of the prior art and provide mycophenolic acid for preparing a medicine for preventing/treating phytophthora litchi (A), (B) and (C)Peronophythora litchii) The use of the pharmaceutical composition of (1).
The fifth purpose of the invention is to overcome the defects of the prior art and provide a method for preventing/treating Peronophythora litchi (R) ((R))Peronophythora litchii) The medicament of (1), wherein the medicament contains mycophenolic acid.
The sixth purpose of the invention is to overcome the defects of the prior art and provide a method for preventing/treating Peronophythora litchi (R) ((R))Peronophythora litchii) The medicine for treating litchi downy blight contains mycophenolic acid.
In order to achieve the purpose, the invention is realized by the following technical scheme:
application of mycophenolic acid in preventing/treating litchi downy blight.
Application of mycophenolic acid in preparation of a medicament for preventing/treating litchi downy blight.
Application of mycophenolic acid in preventing/treating phytophthora litchi (A) and BPeronophythora litchii) The use of (1).
Preparation of mycophenolic acid for preventing/treating peronophythora litchi: (A)Peronophythora litchii) The use of the pharmaceutical composition of (1).
Preferably, the minimum effective inhibitory concentration of mycophenolic acid is 0.1 μ g/m L.
Preferably, the litchi downy blight is caused by Phytophthora litchi.
Preferably, the application is inhibition of phytophthora litchi hypha growth.
Preferably, the application is inhibition of phytophthora litchi sporangium germination.
Peronophythora litchi (A) and Peronophythora litchi (B)Peronophythora litchii) The medicament of (1), the formulation contains mycophenolic acid.
Peronophythora litchi (A) and Peronophythora litchi (B)Peronophythora litchii) The formulation of the medicine for treating litchi downy blight contains mycophenolic acid.
Wherein, mycophenolic acid [ E-4-methyl-6- (1, 3-dihydro-7-methyl-4-hydroxy-6-methoxy-3-oxo-5-isobenzofuranyl) -4-hexenoic acid ] has a molecular formula as follows: C17H20O 6; the chemical formula structure is:
。
compared with the prior art, the invention has the following beneficial effects:
the invention discovers for the first time that: the mycophenolic acid can effectively inhibit the growth of pathogenic bacteria of the peronophythora litchi, has important application in preventing and treating the peronophythora litchi, and particularly can inhibit the growth of peronophythora litchi hyphae and inhibit the germination of peronophythora litchi sporangia, thereby effectively inhibiting the occurrence of the peronophythora litchi.
Moreover, mycophenolic acid can inhibit the growth of pathogenic bacteria but can not completely kill the pathogenic bacteria, which shows that the mycophenolic acid has low toxicity and is more environment-friendly. Therefore, the method has good application prospect in the aspect of preventing and treating the litchi frost blight.
Drawings
FIG. 1 shows the inhibition of mycophenolic acid in the plate on the growth of phytophthora litchi hyphae, and the colony growth of phytophthora litchi in the control group at different concentrations and without medicine.
FIG. 2 shows the growth inhibition rate of Phytophthora litchi hyphae in mycophenolic acid treatment groups with different concentrations and in control groups without drug.
FIG. 3 shows the hypha morphology of Phytophthora litchi in mycophenolic acid-treated group and control group.
FIG. 4 shows the germination of phytophthora litchi sporangia in the mycophenolic acid-treated group and the control group at different concentrations.
FIG. 5 shows the morphology of the germinating germ tubes of Phytophthora litchi sporangia in the mycophenolic acid-treated group and the control group at different concentrations.
Detailed Description
The invention is described in further detail below with reference to the drawings and specific examples, which are provided for illustration only and are not intended to limit the scope of the invention. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1 Activity test of mycophenolic acid for inhibiting the growth of Peronospora litchi hyphae
1. Culture medium: carrot juice agar culture medium CA and potato glucose agar culture medium PDA are used for culturing phytophthora litchi.
2. The inhibitory action of compound mycophenolic acid with different concentrations on the hyphal growth of pathogenic bacteria is measured by a hyphal growth rate method.
The method comprises the following specific operations:
(1) dissolving a pure product of the compound mycophenolic acid in DMSO, and preparing solutions with different gradient concentrations as test solutions.
(2) Adding a certain amount of prepared test solution into a CA culture medium with the temperature reduced to 50 ℃ after melting, uniformly mixing to ensure that the final concentrations of compounds in the culture medium are respectively 0.1, 0.5, 1,3, 5, 10, 20, 30, 100 and 200 mug/m L, setting 3 times for each concentration treatment, adding a blank solvent with the same volume as a control, and pouring the mixture into a culture dish with the diameter of 9 cm by the amount of 10 m L/dish.
(3) And taking Phytophthora litchi which is cultured on a CA culture medium for 5 days, using a sterilized puncher to punch a bacterial cake (d =5 mm) at the edge of a bacterial colony, keeping the bacterial age consistent, and inversely placing the bacterial cake in the center of a culture plate containing compounds with different concentrations, and placing the culture plate in a constant-temperature incubator at 25 ℃ for culture.
(4) The colony diameter is recorded by a cross method through regular observation, and the inhibition rate is calculated.
3. As a result:
FIG. 1 shows that the compound has a significant inhibition effect on pathogenic bacteria of peronophythora litchi, when the concentration of the compound reaches 200 [ mu ] g/m L, the inhibition rate reaches 85%, the inhibition effect on the growth of peronophythora litchi hyphae under different concentrations of mycophenolic acid is shown in FIG. 1, compared with a blank control, the hyphae grows slowly under 20 [ mu ] g/m L, when the concentration is reduced again for screening, the inhibition effect is found to exist under 0.1 [ mu ] g/m L, the inhibition rate reaches 16%, the colony of peronophythora litchi in a treatment group is small, the edge of the colony is irregular, and the hyphae is picked and observed under a microscope, so that the peronophythora litchi is found to be abnormal in shape, thick and short in shape, wrinkled and twisted, the top hyphae is blunt and cannot normally branch, and the malformation is increased as the concentration of the.
Example 2 Activity test of mycophenolic acid for inhibiting Peronospora litchi sporangia Germination
1. Preparation of pathogen sporangia suspension:
culturing Peronophythora litchi in a PDA culture medium for 5 days, adding 5 m L sterile water on a flat plate for washing and shaking to prepare a sporangium suspension mother solution, adding sterile water into the sporangium suspension mother solution to obtain diluted Peronophythora litchi sporangia with the concentration of 105And each m L.
2. The spore germination method is used for determining the inhibition effect of the compound mycophenolic acid with different concentrations on the germination of phytophthora litchi sporangium.
The method comprises the following specific operations:
(1) the compounds were dissolved in DMSO to prepare test solutions having concentrations of 1 mg/m L and 100. mu.g/m L, respectively.
(2) Accurately adding a certain volume of test solution into the diluted sporangia suspension, and fully mixing to ensure that the final concentrations of the compounds in the sporangia suspension are respectively 30, 40 and 50 mu g/m L, and taking the sporangia suspension added with the same volume of solvent as a control.
(3) The sporangium suspension of each treatment group was cultured in a constant temperature incubator at 28 ℃.
(4) Periodically observing the sporangial germination under a microscope, setting 3 replicates for each treatment group, counting about 100 sporangia in three visual field areas for each replicate, and counting the sporangial germination rate.
3. As a result:
as shown in figure 4, the inhibitory effect of the compound mycophenolic acid on the germination of phytophthora litchi sporangia is obviously higher than that of the control group, the inhibitory rates on the germination of the phytophthora litchi sporangia respectively reach 79.8%, 79.9% and 83.1% under the compound concentrations of 30 [ mu ] g/m L, 40 [ mu ] g/m L and 50 [ mu ] g/m L, and the compound mycophenolic acid shows extremely strong antibacterial activity.
Claims (8)
1. Application of mycophenolic acid in preventing/treating litchi downy blight.
2. Application of mycophenolic acid in preparation of a medicament for preventing/treating litchi downy blight.
3. Application of mycophenolic acid in preventing/treating phytophthora litchi (Peronophythora litchii) is provided.
4. Application of mycophenolic acid in preparation of medicament for preventing/treating Peronophythora litchii (Peronophythora litchii).
5. The use of any one of claims 1 to 4, wherein the minimum effective inhibitory concentration of mycophenolic acid is 0.1 μ g/m L.
6. The use of claim 1 or 2, wherein the peronophythora litchi is caused by peronophythora litchi.
7. The use according to claim 3 or 4, wherein the use is for inhibiting the growth of phytophthora litchi hyphae.
8. The use according to claim 3 or 4, wherein the use is for inhibiting phytophthora litchi sporangia germination.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711160719.1A CN107897186B (en) | 2017-11-20 | 2017-11-20 | Application of mycophenolic acid in inhibition of litchi downy blight |
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| CN201711160719.1A CN107897186B (en) | 2017-11-20 | 2017-11-20 | Application of mycophenolic acid in inhibition of litchi downy blight |
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| CN107897186B true CN107897186B (en) | 2020-08-07 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111973600B (en) * | 2020-08-20 | 2021-11-16 | 华中农业大学 | Application of mycophenolic acid or derivative thereof in preparation of canine distemper virus inhibitor |
| CN114686558A (en) * | 2022-04-14 | 2022-07-01 | 广东省农业科学院果树研究所 | Method for detecting litchi downy mildew resistance of litchi and application of method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3880995A (en) * | 1973-05-14 | 1975-04-29 | Lilly Co Eli | Treatment of arthritis with mycophenolic acid and derivatives |
| CN105494378A (en) * | 2015-12-29 | 2016-04-20 | 中国科学院华南植物园 | Application of 6-benaylaminopurine to preparation of pesticide for preventing peronophythora litchi |
| CN106942230A (en) * | 2017-04-21 | 2017-07-14 | 华南农业大学 | Purposes of the mycophenolic acid class compound in smut of sugarcane is prevented and treated |
| CN106962337A (en) * | 2017-04-12 | 2017-07-21 | 江苏金凤凰农化有限公司 | A kind of composition and application process that Peronophythora Litchii is treated based on guaiacol |
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- 2017-11-20 CN CN201711160719.1A patent/CN107897186B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3880995A (en) * | 1973-05-14 | 1975-04-29 | Lilly Co Eli | Treatment of arthritis with mycophenolic acid and derivatives |
| CN105494378A (en) * | 2015-12-29 | 2016-04-20 | 中国科学院华南植物园 | Application of 6-benaylaminopurine to preparation of pesticide for preventing peronophythora litchi |
| CN106962337A (en) * | 2017-04-12 | 2017-07-21 | 江苏金凤凰农化有限公司 | A kind of composition and application process that Peronophythora Litchii is treated based on guaiacol |
| CN106942230A (en) * | 2017-04-21 | 2017-07-14 | 华南农业大学 | Purposes of the mycophenolic acid class compound in smut of sugarcane is prevented and treated |
Non-Patent Citations (3)
| Title |
|---|
| Antiviral activity of mycophenolic acid derivatives in plants;Panattoni A. etal;《Acta Virologica》;20141231;第58卷(第1期);99-102 * |
| Antiviral activity of tiazofurin and mycophenolic acid against Grapevine Leafroll-associated Virus 3 in Vitis vinifera explants;Panattoni A. etal;《Antiviral Research》;20071231;第73卷(第3期);206-211 * |
| Effect of mycophenolic acid on trans-plasma membrane electron transport and electric potential in virus-infected plant tissue;Enrico Rinaldelli etal;《Plant Physiology and Biochemistry》;20121231;第60卷;137-140 * |
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