CN107881828A - A kind of methods of beating using biology enzyme - Google Patents
A kind of methods of beating using biology enzyme Download PDFInfo
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- CN107881828A CN107881828A CN201710966761.6A CN201710966761A CN107881828A CN 107881828 A CN107881828 A CN 107881828A CN 201710966761 A CN201710966761 A CN 201710966761A CN 107881828 A CN107881828 A CN 107881828A
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
The present invention relates to papermaking manufacture field, a kind of methods of beating using biology enzyme is provided, solve the defects of energy height of the methods of beating consumption of prior art, production cost is high, obtained paper physical property is low, enzyme activity site is limited, enzymatic activity is poor, more unstable, including following processing step：(1) it is with slurry：Needle is starched and broad-leaved slurry presses 6~7：4~3 weight obtains mixing slurry than mixing；(2) mashing biology enzyme is prepared：Using modified biology enzyme；(3) mixing slurry pre-processes：The biology enzyme that the modification of the step of 0.2~0.4kg/ tons are starched (2) is added in pulper pre-processes mixing slurry, and at 40~50 DEG C, reaction is re-fed into fiberizer and is beaten temperature control after 2 hours；(4) it is beaten：The deduction degree for being beaten disposed slurry is 75 ° of SR.
Description
Technical field
The present invention relates to papermaking manufacture field, more particularly to a kind of methods of beating using biology enzyme.
Background technology
Mashing need to consume substantial amounts of energy, account for producing the 15%~18% of total energy consumption from timber into paper.Reduce paper pulp
Beating energy consumption, be paper industry energy-saving and emission-reduction a strategic issues.Paper industry energy-conservation has two kinds of approach：1. from management
Aspect reduces energy consumption of unit product；2. reduce energy consumption of unit product using new technology.Wherein, biotechnology is more closed
Note, because it can reduce destruction to environment, save the energy, improves product quality.Compared with general abiotic catalyst, enzyme
There is mildness, selectivity, high efficiency and adjustability.
Enzymatic beating refers to use biology enzyme pretreatment slurry under certain condition, can promote the water suction swollen of fiber and thin
Fibrosis, improved beatability, play a part of reducing beating energy consumption.Have with enzyme reduction beating energy consumption wide
Application prospect, but extensive industrialization of the enzyme to the action rule and enzymatic beating of paper pulp need further to study.
China Patent No. 201410113637.1 discloses a kind of high-strength modified energy-conservation paper pulp of biology enzyme, by following component group
Into：0.5~5 part of antiseptic, 1~3 part of toughener, 1~5 part of intensifier, 40~60 parts of regenerated paper pulp, 10~20 parts of filler, disappear
5~20 parts of 1~5 part of infusion, 2~8 parts of stabilizer, 1~3 part of retention agent, 10~30 parts of fiber and biology enzyme.The patent is using wood
Starch biology enzyme, reduce energy consumption, but the mechanical performance of fiber is poor, the enzyme activity site of biology enzyme is limited, enzymatic activity is poor, compared with
To be unstable.
The content of the invention
Therefore, a kind of methods of beating using biology enzyme is provided, solves beating for prior art for above content, the present invention
The energy of paste-making method consumption is high, production cost is high, obtained paper physical property is low, enzyme activity site is limited, enzymatic activity is poor,
The defects of more unstable.
To reach above-mentioned purpose, the present invention is achieved by the following technical solutions：A kind of mashing side using biology enzyme
Method, including following processing step：
(1) it is with slurry：Needle is starched and broad-leaved slurry presses 6~7：4~3 weight obtains mixing slurry than mixing；
(2) mashing biology enzyme is prepared：Using modified biology enzyme, the biology enzyme of the modification by following parts by weight each original
Material composition：40~45 parts of cellulase, 40~45 parts of hemicellulase, 10~15 parts of laccase, 50~65 parts of histidine, L- phenylpropyl alcohols
40~55 parts of propylhomoserin methyl ester hydrochloride, 1- ethyls -70~80 parts of (3- dimethylaminopropyls) carbodiimide hydrochloride, activated carbon
10~15 parts；
(3) mixing slurry pre-processes：The biology enzyme that the modification of the step of 0.2~0.4kg/ tons are starched (2) is added in pulper is pre-
Mixing slurry is handled, at 40~50 DEG C, reaction is re-fed into fiberizer and is beaten temperature control after 2 hours；
(4) it is beaten：The deduction degree for being beaten disposed slurry is 75 ° of SR.
Further improving is：Be additionally added biological enzyme stabilizers when being beaten in fiberizer, the biological enzyme stabilizers by
Each raw material composition of following parts by weight：30~40 parts of sodium azide, 15~20 parts of sodium benzoate, AEO 40~
55 parts.
Further improving is：The dosage of the biological enzyme stabilizers is 1~3wt% of mixing slurry.
Further improving is：The preparation method of the biology enzyme of the modification is：By laccase and 1- ethyls-(3- dimethyl
Aminopropyl) carbodiimide hydrochloride and L-phenylalanine methyl ester hydrochloride 1~2h of hybrid reaction at room temperature, it is added followed by
Histidine mix, add pH adjusting agent, make pH=4.8~6.0, at room temperature react 0.3~0.5h, add cellulase,
Hemicellulase and activated carbon, are mixed evenly, that is, obtain modified biology enzyme.
By using preceding solution, the beneficial effects of the invention are as follows：The needle slurry and broad-leaved slurry of the present invention presses 6~7：
4~3 weight than mixing, starch and broad-leaved slurry mashing is all relatively difficult, need to consume substantial amounts of energy, the present invention is using modification by needle
Biology enzyme as mashing enzyme add pulper in pretreating paper pulp fiber, addition be 0.2~0.4kg/ tons slurry, temperature control
At 40~50 DEG C, reaction is re-fed into fiberizer after 2 hours and is beaten, and reaches identical beating degree, can reduce mashing power consumption
30%, improve paper physical property；A kind of methods of beating using biology enzyme of the present invention, it uses modified biology enzyme, changed
Property biology enzyme the mechanism of action be the cellulase of particle size very little or hemicellulase preparations enter through cell membrane it is thin
In cell, the appropriateness of cellulose in cell membrane or hemicellulose is degraded by itself, promotes cell wall structure to relax, increases
The permeability of hydrone, promote fibre swelling, drop low-fiber cohesive force, be advantageous to fibrous fracture and wire-dividing broom purification, make fiber
Between have more binding sites, improve paper strength and shaping evenness, reduce the effect of mashing mechanical force；On the other hand, originally
In 1- ethyls of invention-(3- dimethylaminopropyls) carbodiimide hydrochloride activation laccase amino acid residue end carboxyl (-
COOH), the laccase after activation crosslinks reaction with the amino in L-phenylalanine methyl ester hydrochloride and forms peptide bond so that laccase
Active group carboxyl on chain is protected, and laccase native conformation produces certain rigidity, is not easy stretching, extension inactivation, and reduce paint
The thermal vibration of enzyme molecule internal group so that laccase activity, heat endurance are improved；It is added followed by histidine mixing, profit
Be grafted to small-molecule substance on laccase, by increase the active site position of laccase lifted its activity and stability, select laccase/
Histidine system carries out deinking to fiber and is modified the strength character that can greatly promote mixing slurry, because the carboxylic group in histidine
It is remarkably improved fiber electric charge, increases the swollen performance of fiber.
Embodiment
Embodiments of the present invention are described in detail below with reference to specific embodiment, and how skill is applied to the present invention whereby
Art means solve technical problem, and the implementation process for reaching technique effect can fully understand and implement according to this.
Unless otherwise specified, the conventional hand that the technological means employed in embodiment is well known to those skilled in the art
Section, used reagent and product are also available commercial.The source of agents useful for same, trade name and it is necessary to list it
Constituent person, indicate on the first appearance.
Embodiment one
A kind of methods of beating using biology enzyme, including following processing step：
(1) it is with slurry：Needle is starched and broad-leaved slurry presses 6：4 weight obtains mixing slurry than mixing；
(2) mashing biology enzyme is prepared：Using modified biology enzyme, the biology enzyme of the modification by following parts by weight each original
Material composition：40 parts of cellulase, 40 parts of hemicellulase, 10 parts of laccase, 50 parts of histidine, L-phenylalanine methyl ester hydrochloride 40
Part, 1- ethyls -70 parts of (3- dimethylaminopropyls) carbodiimide hydrochloride, 10 parts of activated carbon；The biology enzyme of the modification
Preparation method is：By laccase and 1- ethyls-(3- dimethylaminopropyls) carbodiimide hydrochloride and L-phenylalanine methyl esters salt
Hydrochlorate hybrid reaction 1h at room temperature, histidine mixing is added followed by, pH adjusting agent is added, makes pH=4.8, at room temperature instead
0.3h is answered, cellulase, hemicellulase and activated carbon is added, is mixed evenly, that is, obtains modified biology enzyme.
(3) mixing slurry pre-processes：The biology enzyme pretreatment that the modification of the step of 0.2kg/ tons are starched (2) is added in pulper is mixed
Slurry is closed, at 40 DEG C, reaction is re-fed into fiberizer and is beaten temperature control after 2 hours；
(4) it is beaten：The deduction degree of mashing disposed slurry is 75 ° of SR, is additionally added biological enzyme stabilizers, the biology enzyme during mashing
Stabilizer is made up of each raw material of following parts by weight：30 parts of sodium azide, 15 parts of sodium benzoate, AEO 55
Part.The dosage of the biological enzyme stabilizers is the 1wt% of mixing slurry.
Embodiment two
A kind of methods of beating using biology enzyme, including following processing step：
(1) it is with slurry：Needle is starched and broad-leaved slurry presses 6.5：3.5 weight obtains mixing slurry than mixing；
(2) mashing biology enzyme is prepared：Using modified biology enzyme, the biology enzyme of the modification by following parts by weight each original
Material composition：43 parts of cellulase, 42 parts of hemicellulase, 13 parts of laccase, 62 parts of histidine, L-phenylalanine methyl ester hydrochloride 50
Part, 1- ethyls -75 parts of (3- dimethylaminopropyls) carbodiimide hydrochloride, 12 parts of activated carbon；The biology enzyme of the modification
Preparation method is：By laccase and 1- ethyls-(3- dimethylaminopropyls) carbodiimide hydrochloride and L-phenylalanine methyl esters salt
Hydrochlorate hybrid reaction 1.5h at room temperature, histidine mixing is added followed by, pH adjusting agent is added, makes pH=5.2, at room temperature
0.4h is reacted, cellulase, hemicellulase and activated carbon is added, is mixed evenly, that is, obtain modified biology enzyme.
(3) mixing slurry pre-processes：The biology enzyme pretreatment that the modification of the step of 0.3kg/ tons are starched (2) is added in pulper is mixed
Slurry is closed, at 45 DEG C, reaction is re-fed into fiberizer and is beaten temperature control after 2 hours；
(4) it is beaten：The deduction degree of mashing disposed slurry is 75 ° of SR, is additionally added biological enzyme stabilizers, the biology enzyme during mashing
Stabilizer is made up of each raw material of following parts by weight：35 parts of sodium azide, 17 parts of sodium benzoate, AEO 50
Part.The dosage of the biological enzyme stabilizers is the 2wt% of mixing slurry.
Embodiment three
A kind of methods of beating using biology enzyme, including following processing step：
(1) it is with slurry：Needle is starched and broad-leaved slurry presses 7：3 weight obtains mixing slurry than mixing；
(2) mashing biology enzyme is prepared：Using modified biology enzyme, the biology enzyme of the modification by following parts by weight each original
Material composition：45 parts of cellulase, 45 parts of hemicellulase, 15 parts of laccase, 65 parts of histidine, L-phenylalanine methyl ester hydrochloride 55
Part, 1- ethyls -80 parts of (3- dimethylaminopropyls) carbodiimide hydrochloride, 15 parts of activated carbon；The biology enzyme of the modification
Preparation method is：By laccase and 1- ethyls-(3- dimethylaminopropyls) carbodiimide hydrochloride and L-phenylalanine methyl esters salt
Hydrochlorate hybrid reaction 2h at room temperature, histidine mixing is added followed by, pH adjusting agent is added, makes pH=6.0, at room temperature instead
0.5h is answered, cellulase, hemicellulase and activated carbon is added, is mixed evenly, that is, obtains modified biology enzyme.
(3) mixing slurry pre-processes：The biology enzyme pretreatment that the modification of the step of 0.4kg/ tons are starched (2) is added in pulper is mixed
Slurry is closed, at 50 DEG C, reaction is re-fed into fiberizer and is beaten temperature control after 2 hours；
(4) it is beaten：The deduction degree of mashing disposed slurry is 75 ° of SR, is additionally added biological enzyme stabilizers, the biology enzyme during mashing
Stabilizer is made up of each raw material of following parts by weight：40 parts of sodium azide, 20 parts of sodium benzoate, AEO 40
Part.The dosage of the biological enzyme stabilizers is the 3wt% of mixing slurry.
Wherein, in the present invention, the present invention can be achieved in each raw material of the biology enzyme of the modification in following parameter area
Purpose：40~45 parts of cellulase, 40~45 parts of hemicellulase, 10~15 parts of laccase, 50~65 parts of histidine, L- phenylpropyl alcohols
40~55 parts of propylhomoserin methyl ester hydrochloride, 1- ethyls -70~80 parts of (3- dimethylaminopropyls) carbodiimide hydrochloride, activated carbon
10~15 parts.The higher decomposing force to fiber of the amount of cellulase and hemicellulase is stronger, and histidine can be grafted to laccase
In, increase the avtive spot of laccase, so as to lift its activity and stability, in above range value, the amount of histidine is higher, can
Make laccase more stable.
Described in above, only this wound is used using the embodiment of this origination techniques content, any those skilled in the art
Make done modifications and changes, all belong to the scope of the claims of this creation opinion, and be not limited to those disclosed embodiments.
Claims (4)
1. a kind of methods of beating using biology enzyme, it is characterised in that including following processing step：
(1) it is with slurry：Needle is starched and broad-leaved slurry presses 6~7：4~3 weight obtains mixing slurry than mixing；
(2) mashing biology enzyme is prepared：Using modified biology enzyme, the biology enzyme of the modification by following parts by weight each raw material group
Into：40~45 parts of cellulase, 40~45 parts of hemicellulase, 10~15 parts of laccase, 50~65 parts of histidine, L-phenylalanine
40~55 parts of methyl ester hydrochloride, 1- ethyls -70~80 parts of (3- dimethylaminopropyls) carbodiimide hydrochloride, activated carbon 10~
15 parts；
(3) mixing slurry pre-processes：The biology enzyme pretreatment of the modification of the step of 0.2~0.4kg/ tons are starched (2) is added in pulper
Mixing slurry, at 40~50 DEG C, reaction is re-fed into fiberizer and is beaten temperature control after 2 hours；
(4) it is beaten：The deduction degree for being beaten disposed slurry is 75 ° of SR.
A kind of 2. methods of beating using biology enzyme according to claim 1, it is characterised in that：When being beaten in fiberizer
Biological enzyme stabilizers are additionally added, the biological enzyme stabilizers are made up of each raw material of following parts by weight：30~40 parts of sodium azide,
15~20 parts of sodium benzoate, 40~55 parts of AEO.
A kind of 3. methods of beating using biology enzyme according to claim 2, it is characterised in that：The biological enzyme stabilizers
Dosage be mixing slurry 1~3wt%.
A kind of 4. methods of beating using biology enzyme according to claim 1, it is characterised in that：The biology enzyme of the modification
Preparation method be：By laccase and 1- ethyls-(3- dimethylaminopropyls) carbodiimide hydrochloride and L-phenylalanine methyl esters
Hydrochloride 1~2h of hybrid reaction at room temperature, histidine mixing is added followed by, pH adjusting agent is added, makes pH=4.8~6.0,
0.3~0.5h is reacted at room temperature, is added cellulase, hemicellulase and activated carbon, is mixed evenly, that is, is changed
The biology enzyme of property.
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| CN201710966761.6A CN107881828A (en) | 2017-10-17 | 2017-10-17 | A kind of methods of beating using biology enzyme |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111317165A (en) * | 2020-03-09 | 2020-06-23 | 广东韶关国润再造烟叶有限公司 | Processing method of sandwich type functional tobacco sheet |
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| US5202249A (en) * | 1991-08-08 | 1993-04-13 | Imperial Chemical Industries Plc | Xylanase for biobleaching |
| CN101967470A (en) * | 2010-11-10 | 2011-02-09 | 南京林业大学 | Cellulase stabilizing auxiliary agent composition |
| CN102154867A (en) * | 2010-12-29 | 2011-08-17 | 维达纸业(孝感)有限公司 | Pretreatment method of household paper pulp |
| CN103436501A (en) * | 2013-08-27 | 2013-12-11 | 华南理工大学 | Laccase modification method on basis of amino acid terminal carboxyl group and application thereof |
| CN103642774A (en) * | 2013-11-13 | 2014-03-19 | 宁夏夏盛实业集团有限公司 | Mixed neutral cellulase, preparation method thereof and application thereof to papermaking beating |
| CN103821022A (en) * | 2014-03-11 | 2014-05-28 | 山东尤特尔生物科技有限公司 | Needle pulp and broad leaf pulp pretreatment method with neutral cellulase |
| CN105155324A (en) * | 2015-09-23 | 2015-12-16 | 山东尤特尔生物科技有限公司 | Application of chaetomium thermophilum cellulase in pulping and papermaking process |
-
2017
- 2017-10-17 CN CN201710966761.6A patent/CN107881828A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5202249A (en) * | 1991-08-08 | 1993-04-13 | Imperial Chemical Industries Plc | Xylanase for biobleaching |
| CN101967470A (en) * | 2010-11-10 | 2011-02-09 | 南京林业大学 | Cellulase stabilizing auxiliary agent composition |
| CN102154867A (en) * | 2010-12-29 | 2011-08-17 | 维达纸业(孝感)有限公司 | Pretreatment method of household paper pulp |
| CN103436501A (en) * | 2013-08-27 | 2013-12-11 | 华南理工大学 | Laccase modification method on basis of amino acid terminal carboxyl group and application thereof |
| CN103642774A (en) * | 2013-11-13 | 2014-03-19 | 宁夏夏盛实业集团有限公司 | Mixed neutral cellulase, preparation method thereof and application thereof to papermaking beating |
| CN103821022A (en) * | 2014-03-11 | 2014-05-28 | 山东尤特尔生物科技有限公司 | Needle pulp and broad leaf pulp pretreatment method with neutral cellulase |
| CN105155324A (en) * | 2015-09-23 | 2015-12-16 | 山东尤特尔生物科技有限公司 | Application of chaetomium thermophilum cellulase in pulping and papermaking process |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111317165A (en) * | 2020-03-09 | 2020-06-23 | 广东韶关国润再造烟叶有限公司 | Processing method of sandwich type functional tobacco sheet |
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Application publication date: 20180406 |