CN107854504A - A kind of application of Bai le extract in anti-inflammatory, analgesia and/or haemostatic medicament is prepared - Google Patents
A kind of application of Bai le extract in anti-inflammatory, analgesia and/or haemostatic medicament is prepared Download PDFInfo
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- 239000000284 extract Substances 0.000 title claims abstract description 69
- 239000003814 drug Substances 0.000 title claims abstract description 29
- 230000003110 anti-inflammatory effect Effects 0.000 title claims abstract description 26
- 230000036592 analgesia Effects 0.000 title claims abstract description 19
- 230000000025 haemostatic effect Effects 0.000 title description 5
- 229940030225 antihemorrhagics Drugs 0.000 title description 4
- 239000000853 adhesive Substances 0.000 claims abstract description 7
- 230000001070 adhesive effect Effects 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- 238000000605 extraction Methods 0.000 claims description 28
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 27
- 239000000287 crude extract Substances 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 21
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 14
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 14
- 239000000499 gel Substances 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 11
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
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- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 16
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- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
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- BWZOPYPOZJBVLQ-UHFFFAOYSA-K aluminium glycinate Chemical class O[Al+]O.NCC([O-])=O BWZOPYPOZJBVLQ-UHFFFAOYSA-K 0.000 description 1
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- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- JGMJQSFLQWGYMQ-UHFFFAOYSA-M sodium;2,6-dichloro-n-phenylaniline;acetate Chemical compound [Na+].CC([O-])=O.ClC1=CC=CC(Cl)=C1NC1=CC=CC=C1 JGMJQSFLQWGYMQ-UHFFFAOYSA-M 0.000 description 1
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- 235000000346 sugar Nutrition 0.000 description 1
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- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a kind of application of Bai le extract in the medicine with anti-inflammatory, analgesia and/or anastalsis is prepared.Bai le extract provided by the invention has stronger anti-inflammatory, analgesia, styptic activity, and more precisely, security is higher for curative effect.Other auxiliary materials can be combined, the products such as hemostatic gauze, gel, adhesive bandage are made, applied to fields such as medical dressing, medicines.
Description
Technical field
The invention belongs to medical treatment and nursing technical field, and in particular to a kind of Bai le extract prepare anti-inflammatory, analgesia and/or
Application in haemostatic medicament.
Background technology
White le as medical and edible dual purpose plant in China's history century-old on,《Compendium of Materia Medica》And《Legendary god of farming's book on Chinese herbal medicine
Through》In it is on the books.Bai le bitter, pungent, cold nature, there is clearing heat and detoxicating, wind-dispelling by wet, tonifying middle-Jiao and Qi, hard muscle and other effects, it is interior
Containing chemical compositions such as phenols, general flavone, total saposins, volatile oil, polysaccharide, protein, crude fibre, reduced sugars.In recent years, both at home and abroad
Main Dui Bai le carries out effective substance further investigation, it was demonstrated that and Bai le has the medical value such as anti-oxidant, antibacterial, antitumor,
Have broad application prospects.
Domestic and foreign scholars Dui Bai le research at present is concentrated mainly on the optimal extraction technology of active component in Bai le and resisted
Scorching, antitumor research, almost not to analgesia, the research of styptic activity.
The content of the invention
In view of this, the technical problem to be solved in the present invention be to provide a kind of Bai le extract prepare with anti-inflammatory,
Application in the medicine of analgesia and/or anastalsis, Bai le extract provided by the invention have stronger anti-inflammatory, analgesia, stopped
Blood activity, more precisely, security is higher for curative effect.Other auxiliary materials can be combined, the products such as hemostatic gauze, gel, adhesive bandage are made, should
For fields such as medical dressing, medicines.
The invention provides a kind of Bai le extract in the medicine with anti-inflammatory, analgesia and/or anastalsis is prepared
Using.
Preferably, the Bai le extract is prepared as follows：
Extractant is made with petroleum ether, chloroform, ethyl acetate, n-butanol and sequentially or separately extracts the Bai le crude extract,
Obtain Bai le extracts.
Preferably, the Bai le crude extract is prepared as follows：
The Bai le crude extract is by extraction, reflux extraction, microwave assisting method, ultrasonic wave added method and supercritical CO2Extraction
One or more extracting methods in following the example of are prepared, and the solvent used in the extracting method is selected from ethanol and/or water.
Preferably, the solvent is selected from ethanol water, and the percent by volume of the ethanol water is 30%~95%.
Preferably, the medicine is prepared You Bai le extract and pharmaceutically acceptable auxiliary material.
Preferably, the formulation of the medicine be selected from capsule, pill, powder, tablet, granule, injection, oral liquid,
Paste, aqua, creme, spray-filming agent, gel, liposome, adhesive bandage or dressing.
Compared with prior art, there is anti-inflammatory, analgesia and/or hemostasis in preparation the invention provides a kind of Bai le extract
Application in the medicine of effect.Bai le extract provided by the invention has stronger anti-inflammatory, analgesia, styptic activity, and curative effect is more
Definitely, security is higher.Other auxiliary materials can be combined, the product such as hemostatic gauze, gel, adhesive bandage is made, applied to medical dressing,
The fields such as medicine.
Brief description of the drawings
Fig. 1 is the anti-inflammatory experimental result picture of Bai le extracts of the embodiment of the present invention；
Fig. 2 is the anti-inflammatory Experimental Pathology slice map of Bai le extracts of the embodiment of the present invention；
Fig. 3 is that the anti-inflammatory of Bai le extracts of the embodiment of the present invention tests elisa assay result figure；
Fig. 4 is the analgesic experiment result figure of Bai le extracts of the embodiment of the present invention.
Embodiment
The invention provides a kind of Bai le extract in the medicine with anti-inflammatory, analgesia and/or anastalsis is prepared
Using.
In the present invention, the Bai le extract is prepared as follows：
Make extractant with petroleum ether, chloroform, ethyl acetate, n-butanol and water and sequentially or separately extract the Bai le slightly to carry
Thing (EAT), obtain Bai le extracts.
Wherein, make extractant with petroleum ether, chloroform, ethyl acetate, n-butanol and water and individually extract the Bai le slightly to carry
What thing obtained is respectively Bai le ligroin extraction (PEL), Bai le chloroform extract (CL), Bai le ethyl acetate extracts
(EAL (NBL) are He Bai le water extract (WL) for), Bai le n-butanol extract.
In the present invention, the extracting process is specially：
Bai le crude extract described in Jiang mixes with the extractant, is separated after stratification, obtains Bai le extracts.
In the present invention, the extraction is carried out under normal temperature condition, preferably 20~30 DEG C.
Wherein, the Bai le coarse extraction can mix with extractant in form of an aqueous solutions, can also be with dry powder
Form mixes with extractant.
Dang Bai le crude extract in form of an aqueous solutions in the presence of, the aqueous solution Zhong Bai le crude extracts of the Bai le crude extract
Mass concentration is 10%~80%, and the aqueous solution of the Bai le crude extract and the volume ratio of extractant are preferably 1：1.
The mass body of , Bai le crude extracts and extractant when Bai le crude extract is mixed with dry powder type with extractant
Product ratio preferably 1g：10mL.
In the present invention, the Bai le crude extract is prepared as follows：
The Bai le crude extract is by extraction, reflux extraction, microwave assisting method, ultrasonic wave added method and supercritical CO2Extraction
One or more extracting methods in following the example of are prepared, and the solvent used in the extracting method is selected from ethanol and/or water.
Specifically, Jiang Bai le crushed after being dried, Bai le powder is obtained；Wherein, the Bai le be selected from Bai le leaf, Bai le stems and
One or more in Bai le roots.
Jiang described in after Bai le powder dries, using extraction, reflux extraction, microwave assisting method, ultrasonic wave added method and super
Critical CO2One or more in extraction are extracted, and obtain Bai le crude extracts.
Wherein, the solvent used in the extracting method is selected from ethanol and/or water.In certain specific embodiments of the invention
In, the solvent is selected from ethanol water, and the percent by volume of the ethanol water is 30%~95%, preferably 40%~
85%.
In the present invention, after obtaining the Bai le extract, Jiang described in Bai le extract and pharmaceutically acceptable auxiliary material
The medicine with anti-inflammatory, analgesia and/or anastalsis is prepared.
Wherein, the formulation of the medicine is selected from capsule, pill, powder, tablet, granule, injection, oral liquid, spray
Film, gel of ascending the throne, liposome, adhesive bandage or dressing, preferably ascend the throne gel, adhesive bandage or dressing.
The present invention carries out extraction purification by the active site of preferable extracting method Dui Bai le anti-inflammatory, analgesia, hemostasis, adds
Work technique is simple, can carry out at normal temperatures and pressures, superior in quality beneficial to popularization and application, and product after purification is with stronger
Anti-inflammatory, analgesia, styptic activity, more precisely, security is higher for curative effect.Other auxiliary materials can be combined, hemostatic gauze, gel, wound is made
It the product such as can paste, applied to fields such as medical dressing, medicines.
It is anti-in preparation to Bai le extract provided by the invention with reference to embodiment for a further understanding of the present invention
Application in scorching, analgesia and/or haemostatic medicament is illustrated, and protection scope of the present invention is not limited by the following examples.
Embodiment 1
The preparation of Bai le gels
By Xin Xian Bai le stems in 80 DEG C of dry 24h, after crushed with pulverizer and cross 100 mesh sieves, 4h is dried at 105 DEG C, is obtained
Bai le powder.Accurately weigh 20g powder to be placed in 500mL round-bottomed flasks, add at 80 DEG C of 200mL95% ethanol solutions and flow back
2h is extracted, continues refluxing extraction after filtering, above extraction process is carried out 3 times altogether.Extract obtained rotary evaporation is concentrated and true
Sky is dried to constant weight, is weighed, get Bai le crude extracts (EAT).
At normal temperatures, extractant is made with petroleum ether, chloroform, ethyl acetate, n-butanol and water Yu Bai le crude extract according to
Volume ratio 1：1 is extracted successively, respectively obtains Bai le ligroin extraction (PEL), Bai le chloroform extract (CL), Bai le second
((NBL) are He Bai le water extract (WL) for EAL), Bai le n-butanol extract for acetoacetic ester extract.
The carbomer for taking 2g mass concentrations to be 0.5%, is dissolved in 3mL distilled water, stirs, and makes its swelling as solidifying
Jelly matrix.Bai le chloroform extract (CL) 0.1g obtained above is separately taken, is dissolved in 2.5mL propane diols, fills extract
It is added to after dividing dissolving in gel-type vehicle, adds appropriate triethanolamine regulation pH to 8.0, produce and lived with anti-inflammatory, analgesia, hemostasis
The gel of property.
Embodiment 2
The preparation of Bai le ointment
By 10g Xin Xian Bai le roots in 80 DEG C of dry 24h, after crushed with pulverizer and cross 100 mesh sieves, 4h is dried at 105 DEG C,
Get Bai le powder.Using 85% ethanol as entrainer, supercritical CO is used2Extraction, it is standby that the dregs of a decoction after extraction are vaporized solvent.Extract solution
Ethanol is recovered under reduced pressure in less than 60 DEG C, Bai le crude extracts are made.
Under normal temperature, extractant is made with petroleum ether, chloroform, ethyl acetate, n-butanol and water Yu Bai le crude extracts are according to body
Product ratio 1:1 extracts respectively, and obtaining Bai le ligroin extraction, ((CL), Bai le ethyl acetate extracts PEL), Bai le chloroform extract
((NBL) are He Bai le water extract (WL) for EAL), Bai le n-butanol extract for thing.
6g Sodium Polyacrylates are added in 30g glycerine and stirred, are A phases；0.7g carbomer dispersions are filled in water
Divide swelling, then add 0.3mL tartaric acid, add 0.3g Dihydroxyaluminium Aminoacetates, be B phases；Bai le ethyl acetate obtained above is taken to carry
Take thing (EAL) medicinal extract 2g to add in A phases obtained above, then B phases obtained above are slowly added to A phases, and three ethanol are added dropwise
Amine is mixed evenly to neutrality, adherent layer is covered after coating, obtain the gel ointment with anti-inflammatory, analgesia, styptic activity.
Embodiment 3
Anti-inflammatory activity research
By 100g Xin Xian Bai le bases of leaf (sophisticated 20cm) in 80 DEG C of dry 24h, after crushed with pulverizer and cross 100 mesh sieves,
4h get Bai le powder is dried at 105 DEG C.By 1:The ethanol that 15 solid-liquid ratio volume fraction is 40% soaks, ultrasonic power 80w,
2h is extracted, is filtered.Ultrasound assisted extraction step 2 time is repeated, merging filtrate, ethanol to filtrate is recovered under reduced pressure and is evaporated, gained extraction
Thing Wei Bai le crude extracts.
Under normal temperature, extractant is made with petroleum ether, chloroform, ethyl acetate, n-butanol and water Yu Bai le crude extracts are according to body
Product ratio 1：1 is extracted successively, respectively obtains Bai le ligroin extraction (PEL), Bai le chloroform extract (CL), Bai le acetic acid
((NBL) are He Bai le water extract (WL) for EAL), Bai le n-butanol extract for ethyl ester extract.
Balb/c male mices, 18~22g of body weight are taken, stochastic averagina is divided into 16 groups, and every group 4, i.e., blank control group is (pure
Acetone) 1 group, 6 groups of 1 group of TPA (phorbol exters) control group, medicine group (Ji Bai le crude extract (EAT) are He Bai le extracts：Bai le stones
Oily ether extract (PEL), Bai le chloroform extract (CL), Bai le ethyl acetate extract (EAL), Bai le n-butanol extracts
(NBL) He Bai le water extract (WL)), 2 drug concentrations of each medicine setting are respectively：50th, 100mg/kg, medicine group is added up to
There are 14 groups.Each group medicine is with acetone solution.Tested according to TPA- inducing mouse ear swelling models, by comparative drug group,
TPA control groups and naive mice ear restore anti-inflammatory result, and as shown in Figure 1, Fig. 1 extracts for Bai of embodiment of the present invention le
The anti-inflammatory experimental result picture of thing.In addition, carrying out paraffin section analysis to ear, obtained histotomy figure as shown in Figure 2, is schemed
2 be the anti-inflammatory Experimental Pathology slice map of Bai le extracts of the embodiment of the present invention.Another ear is used as ELISA experimental analyses,
Inflammatory factor IL-1 β concentration is determined, absorbance is determined at 450nm using ELIASA, combined standard curve method is analyzed to obtain
As a result as shown in Figure 3, Fig. 3 is that the anti-inflammatory of Bai le extracts of the embodiment of the present invention tests elisa assay result figure.
Wherein, using Student-Newman-Keuls one-way analysis of variance method analyze datas, as a result middle * p<
0.05、**p<0.01、***p<0.001, p smaller explanation medicine exercising result is more notable compared with blank control group.
With reference to mouse ear weight measurement result, pathological section figure and ELISA testing results, it can be seen that white le crude extract
(EAT (PEL), Bai le chloroform extracts (CL) and Bai le ethyl acetate extract (EAL) exist), Bai le ligroin extraction
50th, the mice ear degree (p of TPA inductions can be significantly reduced under 100mg/kg concentration<0.01) preferable anti-inflammatory, is shown
Activity, wherein, the antiphlogistic effects of chloroform extract are optimal, 50, reach under 100mg/kg concentration nearly 99.9% swelling suppression
Rate processed, the most strong (p of conspicuousness<0.001).Er Bai le n-butanol extracts (NBL) can only have preferably under 100mg/kg concentration
Active , Bai le water extract (WL) is almost without antiphlogistic effects.
Embodiment 4
Analgesic activities are studied
Wistar male rats, 180~220g of body weight are taken, stochastic averagina is divided into 8 groups, and every group 4, i.e. formalin compares
In group, positive controls (diclofenac sodium gel, being denoted as 1%DF), experimental group (i.e. Bai le crude extract (EAT) and embodiment 3
Arrive Bai le extracts：Bai le ligroin extraction (PEL), Bai le chloroform extract (CL), Bai le ethyl acetate extracts
(EAL), Bai le n-butanol extract (NBL) are He Bai le water extract (WL)).Tested and carried out according to rat formalin-induced,
Experiment is licked using animal, stings claw as the index of pain reaction, first stage (0-5min) after formalin injects and the
Two-stage (15-30min) record mouse licks the total degree of claw, observing time with s (second) for unit, as a result as shown in Figure 4,
Fig. 4 is the analgesic experiment result figure of Bai le extracts of the embodiment of the present invention.
Wherein, experiment utilizes Student-Newman-Keuls one-way analysis of variance method analyze datas, as a result middle * p<
0.05、**p<0.01、***p<0.001, p smaller explanation medicine exercising result is more notable compared with control group.
By Fig. 4 formalin-induced experimental results, it can be seen that Bai le difference extracts and positive control antiphen
Sour sodium gel (1%DF) can not effectively reduce the pain of the mouse in the first stage, and the licking of mouse is stung number and be held in
22 times or so.And 5% Bai le difference extracts and 1%DF can significantly reduce what mouse induced by formalin in second stage
Lick and sting the sufficient number of injection, wherein, 5%EAL, CL act on, 5%PEL, NBL, WL action effect and 1%DF more notable than 1%DF
Quite (p<0.05).Therefore, experiment proves that Bai le extracts have good analgesic activities.
Embodiment 5
Styptic activity is studied
4kg new zealand male rabbits 3 are taken, take blood 25mL from auricular vein respectively.By 3.8% liquor sodii citratis and rabbit
Auricular vein blood proportionally 1:9 (w/w) are gently mixed, and obtain anticoagulated blood., will under the conditions of 15 DEG C of centrifugal force 3000r/min
Anticoagulated blood centrifuges 30min.Setting blank group is deionized water, wherein with 0.2775% calcium chloride solution (i.e. M/40 calcium chloride
Solution) it is blood coagulation substance, experimental group is to prepare Bai le alcohol extracts He Bai le extract (Bai le petroleum ethers in embodiment 1
Extract (PEL), Bai le chloroform extract (CL), Bai le ethyl acetate extract (EAL), Bai le n-butanol extracts (NBL)
He Bai le water extracts (WL)) (being each configured to 0.5%, 1.0% and 2.0% 3 concentration) be used as blood coagulation substance, respectively
Blood plasma recalcification time in blank group, positive controls, experimental group is determined, as a result as shown in table 1, table 1 is that the embodiment of the present invention is white
The hemostasis experimental result of le extract.
The hemostasis experimental result of the Bai le extracts of the embodiment of the present invention of table 1
Shown by the experimental result of table 1, compared to 0.5% concentration and 2.0% concentration , Bai le extracts in 1.0% (w/w)
Blood plasma recalcification time is most short under concentration, shows that haemostatic effect is optimal.And drug concentration be 1.0% when, ethyl acetate layer extract
The lower blood plasma recalcification time of effect is most short, about 80s, under being acted on compared to deionized water (blank group), shortens 34.85%；Bai le
Crude extract, Bai le ligroin extraction, Bai le chloroform extracts and Bai le ethyl acetate extract blood plasma recalcification times are also bright
It is aobvious to shorten, Er Bai le n-butanol extract and water extract effect unobvious.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
- A kind of 1. application of Bai le extract in the medicine with anti-inflammatory, analgesia and/or anastalsis is prepared.
- 2. application according to claim 1, it is characterised in that the Bai le extract is prepared as follows：Extractant is made with petroleum ether, chloroform, ethyl acetate, n-butanol and sequentially or separately extracts the Bai le crude extract, is obtained Bai le extracts.
- 3. application according to claim 2, it is characterised in that the Bai le crude extract is prepared as follows：The Bai le crude extract is by extraction, reflux extraction, microwave assisting method, ultrasonic wave added method and supercritical CO2In extraction One or more extracting methods be prepared, the solvent used in the extracting method is selected from ethanol and/or water.
- 4. application according to claim 1, it is characterised in that the solvent is selected from ethanol water, and the ethanol is water-soluble The percent by volume of liquid is 30%~95%.
- 5. application according to claim 1, it is characterised in that the medicine You Bai le extracts with it is pharmaceutically acceptable Auxiliary material is prepared.
- 6. application according to claim 1, it is characterised in that the formulation of the medicine be selected from capsule, pill, powder, Tablet, granule, injection, oral liquid, paste, aqua, creme, spray-filming agent, gel, liposome, adhesive bandage or dressing.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115671131A (en) * | 2022-12-01 | 2023-02-03 | 广东药科大学 | Application of trifoliate acanthopanax polysaccharide in preparation of medicine for treating hyperuricemia |
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| US20060110468A1 (en) * | 2004-11-24 | 2006-05-25 | Liang Liu | Herbal formulations for arthritis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20060110468A1 (en) * | 2004-11-24 | 2006-05-25 | Liang Liu | Herbal formulations for arthritis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115671131A (en) * | 2022-12-01 | 2023-02-03 | 广东药科大学 | Application of trifoliate acanthopanax polysaccharide in preparation of medicine for treating hyperuricemia |
| CN115671131B (en) * | 2022-12-01 | 2023-05-23 | 广东药科大学 | Application of acanthus trifoliatus polysaccharide in preparing medicine for treating hyperuricemia |
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