CN107817231A - A kind of portable detector for being directed to coliform and EHEC - Google Patents
A kind of portable detector for being directed to coliform and EHEC Download PDFInfo
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- CN107817231A CN107817231A CN201710953765.0A CN201710953765A CN107817231A CN 107817231 A CN107817231 A CN 107817231A CN 201710953765 A CN201710953765 A CN 201710953765A CN 107817231 A CN107817231 A CN 107817231A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The invention discloses a kind of portable detector for being directed to coliform and EHEC, fast-field evaluation coliform, EHEC can be achieved, detection sensitivity can reach 2 × 10‑ 16Mol, detection error are less than ± 5%, can store 20000 testing results, 1000 sample plans, each sample plan can realize 3000 sampled points.Relatively conventional surface plate cultivation measure bacterial number needs 24 hours, and the present invention was only needed within 10 seconds；The present invention has small volume compared with traditional bench device, only weigh 380 grams, have the advantages that economical, convenient, fast, time saving, sensitiveness is high, brought great convenience to field monitoring, not only save a large amount of human and material resources, but also monitoring coverage rate can be expanded, it is a kind of comparatively ideal detection device that basic health epidemic prevention organization carries out health supervision to food kitchenware.
Description
Technical field
The invention belongs to technical field of biological, and in particular to a kind of to be directed to coliform and EHEC just
Take formula detector.
Background technology
Microbiologic inhibition tests are to judge the major criterion whether food conforms to quality requirements with hygienic requirements, detection
Index is divided into total number of bacteria detection, coliform detection and pathogenic bacteria detection.All the time both at home and abroad all using coliform as
Food, one of the conventional indicator bacteria of water pollution, evaluation and judge the degree of food faecal contamination and whether there is pathogenic entero becteria
The possibility of pollution.
Conventional detection coliform uses colony counting method (plate count, flat-plate bacterial colony in GB 4789.3-2016 more
Number selection, validating experiment), 3 day time is needed altogether, it is cumbersome, time-consuming, laborious, it is impossible to adapt to the need of a large amount of actually detected work
Will.Rapid fluorescence detection MPN (mostprobable number, maximum MPN) counting method has been put into national standard GB/T
4789.32-2002《Microbiological test of food hygiene, the quick detection of coliform》, make to need 72h report results to reduce originally
To 18~24h.While it is also the judgement coliform bacterium colony of preliminary sxemiquantitative but the method still can not accomplish fast and simple
Number.
Due to that specific beta galactosidase can be produced in coliform growth course, in decomposed liq culture medium
Zymolyte 4-methyl umbelliferone-β-D- galactosides (hereinafter referred to as MUGal), make 4-methyl umbelliferone dissociate, thus in wavelength
366nm ultraviolet lamp irradiation is lower to be presented blue-fluorescence, can be determined by 450nm wavelength.EHEC is in MUG (4- methyl
Umbelliferone glucosiduronic acid) experiment in, using MUG as EHEC basic nutrition thing add culture medium in, by large intestine angstrom wish
The β of bacterium-direct catabolite of glucuronic acid enzyme is used as a kind of instruction system again, and white fluorescent is presented under 366nm ultraviolet lights,
That is MUG is positive；If unstressed configuration, i.e. MUG is negative.
The content of the invention
In view of above-mentioned, the invention provides a kind of portable detector for being directed to coliform and EHEC, tool
There are higher detection sensitivity and the degree of accuracy, compact-sized, small volume, while be also integrated with ATP (Adenosine
Triphosphate, adenosine triphyosphate) fluorescence detection method can determine total plate count.
A kind of portable detector for being directed to coliform and EHEC, including shell and its power supply mould of inside
Block, detection cabin, core detection module and main control board；The power module is used for for all consuming parts inside detector
Supply voltage is provided, the main control board is connected with core detection module, for detecting what is obtained to core detection module
Signal results are handled；The cover top portion is provided with the hatchcover of clamshell, detection cabin one end insertion core detection module
Interior, the other end is communicated to hatchcover so that sample can be imported in core detection module by user after opening hatchcover by detecting cabin
Row detection；
Be provided with the core detection module ATP fluoroscopic examinations part, energizing circuit plate, exciting light detection circuit board and
Excite photodetection assembly；Wherein：
The ATP fluoroscopic examinations part is used to detect the total plate count imported in core detection module on sample, and will inspection
Survey result and be supplied to main control board；
Energizing circuit plate and exciting light the detection circuit board is respectively at left and right sides of detection cabin, and respectively by arranging pin
Interface is connected with main control board；Energizing circuit plate is controlled by main control board, for producing ultraviolet light, is led when by detecting cabin
Enter and there are coliform on the sample in core detection module, blue-fluorescence is presented in it under ultraviolet light, then exciting light
Detection circuit board is determined by wavelength to identify the blue-fluorescence and present the result to main control board；
It is described to excite photodetection assembly to include exciting LED, exciter filter, transparent glass cover and exciting photodetector,
It is described to excite LED and exciter filter to be located at detection cabin side, it is described to excite photodetector positioned at detection cabin opposite side, it is described
Light cloche is set on the detection cabin in insertion core detection module；
Described to excite LED to be used to produce ultraviolet light, the ultraviolet light produces specific after the exciter filter filters out veiling glare
The ultraviolet light of wavelength, when there are EHEC on the sample imported by detecting cabin in core detection module, it is ultraviolet
White fluorescent is presented under light irradiation, then excites photodetector to be determined by wavelength to identify the white fluorescent and present the result to
Main control board.
Further, the case surface is provided with the display screen being connected with main control board and input button, Neng Goujie
Control instruction is inputted by user and visualizes the testing result after treated.
Further, the shell side is provided with the charging inlet being connected with power module so that instrument has charging work(
Energy.
Further, the hatchcover is connected with hatchcover opening and closing detector, and its folding condition for being used to detect hatchcover simultaneously will
Testing result sends main control board to；Instrument can not be detected further if testing result is patent conjunction state, so as to anti-
Influence of the leak-stopping light to testing result simultaneously provides related prompting in display screen.
Further, the detection cabin is provided with sample decision maker close to hatchcover opening position, and it is used to whether detect sample
Insertion detection cabin simultaneously sends testing result to main control board；Instrument can not if testing result is to be not inserted into sample state
Further detect so as to prevent error detection result and provide related prompting in display screen.
Further, the enclosure edge is provided with shading strip, for blocking in extraneous light to detector.
Further, energizing circuit plate and exciting light the detection circuit board is used as by respective metallic shield respectively covers
Lid.
Further, the core detection module is connected with main control board by screening arrangement, so as to respectively be examined internal
Survey the detection signal that device is obtained and be supplied to main control board, and serve signal shielding effect.
Further, described to excite LED to be connected with exciting light feedback detector, it is used to detect the battery electricity for exciting LED
Pressure feeds back to main control board, controls LED current to adjust it by PID (proportional-integral-differential) algorithm by main control board
The light intensity sent, so as to reach permanent optical power control effect.
Further, the detection cabin is provided with locating part, is easy to the plug and shading of nacelle.
Detector of the present invention can realize fast-field evaluation coliform, EHEC, detection sensitivity can reach 2 ×
10-16Mol, detection error are less than ± 5%, can store 20000 testing results, 1000 sample plans, each sample plan
3000 sampled points can be realized.Relatively conventional surface plate cultivation measure bacterial number needs 24 hours, and the present invention only needs 10
Within second；In addition the present invention is once charged using low power processor and can be used continuously 72 hours；With traditional bench device
There is small volume compared to the present invention, only weigh 380 grams, have the advantages that economical, convenient, fast, time saving, sensitiveness is high, to scene
Monitoring brings great convenience, and not only saves a large amount of human and material resources, but also can expand monitoring coverage rate, is that basic unit defends
Raw epidemic prevention organization carries out a kind of comparatively ideal detection device of health supervision to food kitchenware.
Brief description of the drawings
Fig. 1 is the surface structure schematic diagram of detector of the present invention.
Fig. 2 is the internal structure schematic diagram of detector of the present invention.
Fig. 3 is the axonometric drawing of detector internal structure of the present invention.
Fig. 4 is the cross-sectional view of detector core detection module of the present invention.
Embodiment
In order to more specifically describe the present invention, below in conjunction with the accompanying drawings and embodiment is to technical scheme
It is described in detail.
As shown in Figure 1 to 4, the present invention is for coliform and the portable detector of EHEC, main bag
Include power module 23, detection cabin 9, core detection module 13 and the main control board 20 of shell 3 and its inside.Shell 3 is main
Including being used to insert the hatchcover 1 for detecting sample to detection cabin 9 at the top of detector, hatchcover 1 can turn over above the side of LCDs 4
Open 120°On rear side of to detector, charging inlet 2 is located at the left side of shell 3, and it is defeated that the front of shell 3 upside has LCDs 4, downside to have
Enter button 5.
Main control board 20 is located at LCDs 4, control button 5 and detected between cabin 9, core detection module 13, cabin
Lid neck 21 plays extraneous light effect of blocking by particular design with hatchcover 1, and swab 22 is used to gather among detection cabin 9
Sample, there is detection sample decision maker 7 close to the position of hatchcover 1 in detection cabin 9, having hatchcover close to detection cabin 9 and the position of hatchcover 1
Nearby there is chargeable installation region 19 in folding decision maker 6, detection cabin 9, and power module 23 is then placed in one, and detects the bottom of cabin 9
It is inserted among core detection module 13；Shading strip 8 is kept to the side inside shell 3 for blocking extraneous light to detector.
Core detection module 13 has ATP fluoroscopic examinations part 15 to be used to detect total plate count by the side of main control board 20, its
Principle is that the faint optical signal that sample is sent is carried out into opto-electronic conversion via photodiode, then via second amplifying circuit to letter
Number it is amplified, the differential signal after finally amplifying via collection realizes that analog-to-digital conversion is sent by AD sampling A/D chips MAX1407
To processor.
Energizing circuit plate 17 is located at the both sides of core detection module 13 with exciting light detection circuit board 12, perpendicular to governor circuit
Plate 20 is connected by arranging needle interface 14 and 16 respectively, and energizing circuit plate 17 detects circuit board 12 respectively by two blocks of metals with exciting light
Radome 11 and 18 covers.Core detection module 13 is connected by fixed mask device 10 with main control board 20 and serves letter
Number shielding action.Excite LED27, exciter filter 28, transparent glass cover 25, excite photodetector 24 point-blank to form
One complete detection circuit, ATP fluorescence detectors 15 are therewith into 90°Ensure that different detections are non-interference；Exciting light feeds back
Positioned at exciting for adjusting light intensity on the downside of LED27, it is used detector 26 by gathering feedback photocell both end voltage in real time
Pid algorithm adjusts LED control electric currents, so as to reach permanent luminous power；Detection cabin 9 is pressed close to the finite place piece 29 of core detection module 13 and used
In the plug and shading of nacelle.
Power module 23 is used to provide supply voltage for all consuming parts inside detector, main control board 20 for pair
The signal results that core detection module 13 detects to obtain are handled, and it is mainly integrated with charging and discharging lithium battery circuit, voltage stabilizing
Circuit, serial communication module, wifi module, bluetooth module, liquid crystal display drive module, MCU signal processors and AD sampling A/D chips；
Charging and discharging lithium battery circuit can be by external MicroUSB ports to 18650 lithium cell chargings of section；Lithium battery voltage was discharged
Reduce and reduce with electricity in journey, so needing mu balanced circuit burning voltage, powered to miscellaneous part；Serial communication module is TTL
Rs 232 serial interface signal is converted to usb signal, can carry out data upload with PC ends USB interface；Processor is public using ST in present embodiment
Take charge of STM32L103 series low-power chips.
There are a LED and feedback photocell on energizing circuit plate 17, main control board 20 be connected to by arranging needle interface 14,
Main control board 20 gathers feedback light battery signal and controls LED drive current, and LED is used to produce ultraviolet light, is detected when passing through
Coliform is there are on the sample that cabin 9 is imported in core detection module 13, blue-fluorescence is presented in it under ultraviolet light, then
Exciting light detection circuit board 12 is determined by wavelength to identify the blue-fluorescence, and then signal is sent to master by amplifying circuit
Control the AD sampling A/D chips on circuit board 20；LED excites LED using THORLABS companies 370E models in present embodiment.
LED27 is excited to be used to produce the ultraviolet light that centre wavelength is 370nm, the ultraviolet light is 366nm by centre wavelength
Pulsewidth is that 50nm exciter filters 28 filter out and the ultraviolet light of specific wavelength is produced after veiling glare are used to excite sample to produce fluorescence, when logical
Cross on the sample that detection cabin 9 is imported in core detection module 13 and there are EHEC, it is presented in vain under ultraviolet light
Color fluorescence, then photodetector 24 is excited to be determined by wavelength to identify the white fluorescent and present the result to main control board
20；Transparent glass cover 25 is set in the detection bottom of cabin 9, and it is used to isolate sample and detector 24 so as to serve Protective Detector
24 effect, and be easy to clean with removable unloading functions, transparent glass cover 25 also has condenser effect in addition；Examined in present embodiment
Survey photodiode of the device 24 using shore pine S1337 models.
Hatchcover opening and closing detector 6 is used to detect the folding condition of hatchcover 1 and sends testing result to main control board
20, instrument can not be detected further if testing result is patent conjunction state, so as to prevent influence of the light leak to testing result
And provide related prompting in display screen 4.
Sample decision maker 7 is used to detect whether sample inserts detection cabin 9 and send testing result to main control board
20, instrument can not be detected further so as to prevent error detection result simultaneously if testing result is to be not inserted into sample state
Related prompting is provided in display screen 4.
Last core detection module 13 detects obtained chemiluminescence detection signal and fluorescent assay signal is sent to master
The AD sampling A/D chips collection in circuit board 20 is controlled, processor is calculated AD values using SPI communication with AD sampling A/D chips, shown
The following information of screen display：
1. startup self-detection interface：Hatchcover detection, sample detection, ATP backgrounds, fluorescence light source and autofluorescent background；
2. main interface：ATP detections, fluoroscopic examination, instrument is set, data upload；
3. detect interface：ATP and fluoroscopic examination result, detection numbering, limit value sequence number；
4. instrument is set：Screen intensity, keypad sounds, system time；
5. data upload：Serial ports is uploaded, bluetooth uploads, wifi is uploaded.
The above-mentioned description to embodiment is understood that for ease of those skilled in the art and using the present invention.
Person skilled in the art obviously can easily make various modifications to above-described embodiment, and described herein general
Principle is applied in other embodiment without by performing creative labour.Therefore, the invention is not restricted to above-described embodiment, ability
For field technique personnel according to the announcement of the present invention, the improvement made for the present invention and modification all should be in protection scope of the present invention
Within.
Claims (10)
- A kind of 1. portable detector for being directed to coliform and EHEC, it is characterised in that：Including shell and its interior Power module, detection cabin, core detection module and the main control board in portion；The power module is used for for institute inside detector There are consuming parts to provide supply voltage, the main control board is connected with core detection module, for core detection module institute Obtained signal results are detected to be handled；The cover top portion is provided with the hatchcover of clamshell, detection cabin one end insertion core In heart detection module, the other end is communicated to hatchcover so that sample can be imported core inspection by user after opening hatchcover by detecting cabin Survey in module and detected；ATP fluoroscopic examinations part, energizing circuit plate, exciting light detection circuit board are provided with the core detection module and is excited Photodetection assembly；Wherein：The ATP fluoroscopic examinations part is used to detect the total plate count imported in core detection module on sample, and detection is tied Fruit is supplied to main control board；Energizing circuit plate and exciting light the detection circuit board is respectively at left and right sides of detection cabin, and respectively by arranging needle interface It is connected with main control board；Energizing circuit plate is controlled by main control board, for producing ultraviolet light, when by detecting cabin importing core Coliform is there are on sample in heart detection module, blue-fluorescence is presented in it under ultraviolet light, then exciting light detects Circuit board is determined by wavelength to identify the blue-fluorescence and present the result to main control board；It is described to excite photodetection assembly to include exciting LED, exciter filter, transparent glass cover and exciting photodetector, it is described LED and exciter filter are excited positioned at detection cabin side, it is described to excite photodetector positioned at detection cabin opposite side, the printing opacity glass The glass enclosure is located on the detection cabin in insertion core detection module；Described to excite LED to be used to produce ultraviolet light, the ultraviolet light produces specific wavelength after the exciter filter filters out veiling glare Ultraviolet light, when by detect cabin import core detection module in sample on there are EHEC, it is in ultraviolet lighting Lower presentation white fluorescent is penetrated, then excites photodetector to be determined by wavelength to identify the white fluorescent and present the result to master control Circuit board.
- 2. portable detector according to claim 1, it is characterised in that：The case surface is provided with and main control board The display screen and input button of connection.
- 3. portable detector according to claim 1, it is characterised in that：The shell side is provided with to be connected with power module The charging inlet connect.
- 4. portable detector according to claim 1, it is characterised in that：The hatchcover is connected with hatchcover folding detection dress Put, its folding condition for being used to detect hatchcover simultaneously sends testing result to main control board.
- 5. portable detector according to claim 1, it is characterised in that：The detection cabin is provided with close to hatchcover opening position Sample decision maker, it is used to detect whether sample inserts detection cabin and send testing result to main control board.
- 6. portable detector according to claim 1, it is characterised in that：The enclosure edge is provided with shading strip.
- 7. portable detector according to claim 1, it is characterised in that：Energizing circuit plate and exciting light the detection electricity Road plate is respectively by respective metallic shield as covering.
- 8. portable detector according to claim 1, it is characterised in that：The core detection module and main control board Connected by screening arrangement, so as to which the detection signal for being obtained internal each detection device is supplied to main control board.
- 9. portable detector according to claim 1, it is characterised in that：It is described to excite LED to be connected with exciting light feedback Detector, it, which is used to detect, excites LED cell voltage to feed back to main control board, passes through pid algorithm control by main control board LED current processed is to adjust the light intensity that it sends.
- 10. portable detector according to claim 1, it is characterised in that：The detection cabin is provided with locating part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710953765.0A CN107817231B (en) | 2017-10-13 | 2017-10-13 | Portable detector for coliform and escherichia coli |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710953765.0A CN107817231B (en) | 2017-10-13 | 2017-10-13 | Portable detector for coliform and escherichia coli |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107817231A true CN107817231A (en) | 2018-03-20 |
| CN107817231B CN107817231B (en) | 2020-04-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710953765.0A Active CN107817231B (en) | 2017-10-13 | 2017-10-13 | Portable detector for coliform and escherichia coli |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107817231B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112147131A (en) * | 2020-08-20 | 2020-12-29 | 西安交通大学 | Portable microorganism short-term test appearance |
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| CN107817231B (en) | 2020-04-21 |
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