CN107810900A - A kind of small model organism real time imagery and high speed separation system - Google Patents
A kind of small model organism real time imagery and high speed separation system Download PDFInfo
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- CN107810900A CN107810900A CN201711052704.3A CN201711052704A CN107810900A CN 107810900 A CN107810900 A CN 107810900A CN 201711052704 A CN201711052704 A CN 201711052704A CN 107810900 A CN107810900 A CN 107810900A
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- model organism
- small model
- real time
- high speed
- time imagery
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
- A01K61/95—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination specially adapted for fish
Abstract
The invention discloses a kind of small model organism real time imagery and high speed separation system, after illuminator is illuminated to small model organism, small model organism is imaged by imaging identifying system and identifies differentiation, finally small model organism is added dropwise in porous disc using working fluid by screening system.Fast imaging can be carried out to small model organism using the present invention, and carry out scan picture, according to morphological operation, finally small model organism can quickly be screened.
Description
Technical field
The present invention relates to separation system, and in particular to a kind of small model organism real time imagery and high speed separation system.
Background technology
It is at present the small model animal of representative in Developmental Biology, immunology, Neurobiology and medicine using zebra fish
More and more important effect is played in thing screening.But the sorting at present to microminiature model animal is screened still through part
Based on image and manual operations, its workload is big but data acquisition efficiency is low, can not realize extensive analysis in real time, Wu Fayou
Effect plays the intrinsic advantage of small model animal, Large scale genetic/drug screening demand in can not more adapting to.
The content of the invention
The problem to be solved in the present invention is to provide a kind of small model organism real time imagery and high speed separation system, utilized
Optical system first carries out high speed imaging to small model organism embryo, then carries out realtime graphic identification, is drawn by morphological operation
Result, to the dead of small model organism or living, if injection medicine is screened respectively.
To solve the above problems, the present invention provides a kind of small model organism real time imagery and high speed separation system,
To reach above-mentioned purpose, the technical solution adopted for the present invention to solve the technical problems is：
A kind of small model organism real time imagery and high speed separation system, including：Illuminator, imaging identifying system, sieve
Select system, it is characterised in that：Illuminator, it is illuminated using mating plate to small model organism；Identifying system is imaged, its is right
Small model organism in flowing after illuminating carries out real time imagery and identifies differentiation；Screening system, it will using liquid fluid system
Small model organism is instilled in porous disc, realizes the screening of small model organism.
As a further improvement on the present invention, the illuminator includes：Light source, the first collimation lens, cylindrical mirror, second
Collimation lens, object lens, light source, there is provided illuminating bundle；First collimation lens, the light beam that light source is sent are collimated；Cylindrical mirror,
Light beam after first collimation lens is collimated forms cylinder light, and small model organism is imaged；Second collimation lens, by post
The light beam that face mirror goes out is collimated again；First object lens, the light beam projected to the second collimation lens are focused.
As the further improvement of the present invention, the imaging identifying system includes：Micro-injection pump, capillary, imaging
Object lens, lens, line array CCD, computer；Micro-injection pump, micro-injection pump are connected with capillary, to contain small model organism
Liquid provides carrying capacity；Capillary, liquid of the transmission with small model organism；Second object lens, for the light beam through capillary
Pass through；3rd collimation lens, the light beam that the second object lens project is collimated；Line array CCD, small model organism is carried out real-time
Imaging, and imaging is imported into computer；Computer, small model organism is identified according to Morphology Algorithm.
As the further improvement of the present invention, the screening system includes：Displacement platform, conical nozzle, porous disc, displacement
Platform, moved by computer generation instruction come control bit moving stage on space X, Y, Z axis, displacement platform drives capillary movement；Tapered injection
Mouth, it is arranged on the bottom of capillary and is directed at porous disc；Porous disc, possess some independent receiving spaces for accommodating with small
Model organism.
As the further improvement of the present invention, the small model organism is zebra fish.
As the further improvement of the present invention, the cylindrical mirror f=10mm.
As the further improvement of the present invention, the light source is monocrystalline LED.
As the further improvement of the present invention, the first collimation lens f=100mm, the second collimation lens f=
200mm, the 3rd collimation lens f=200mm.
As the further improvement of the present invention, first object lens, the second object lens are 10 × object lens.
As the further improvement of the present invention, the capillary lower end is secured in place in moving stage by clip.
The technical solution adopted in the present invention is；The target design of 10um resolution ratio to reach goes out light path, optical system
Two-dimensional imaging is carried out to zebra fish sample, morphological operation then is carried out to imaging, displacement platform is according to morphological operation knot
Fruit is instructed to move, and different types of zebra fish is sorted into porous disc.
It is using the beneficial effect of above-mentioned technical proposal：
The present invention is streaming systems, and when sample flows through imaging capillary along with liquid stream, another side line array CCD is to sample
Carry out real time imagery；The camera of collection image in the present invention is line array CCD, it is possible to achieve high speed imaging.
Lighting part make use of cylindrical mirror, can form mating plate, and sample is illuminated using mating plate, lighting hours compared with
It is short, therefore light injury is smaller to caused by sample.
Fast imaging can be carried out to the small model organism such as zebra fish, and carry out scan picture, according to morphology
Operation, finally can quickly be screened to zebra fish.
On the one hand from being carried out to zebra fish from embryonic development, the zebra fish of different shape is sub-elected, is carried out respectively
Cultivate.On the other hand live body zebrafish larvae can be screened, the live body zebrafish larvae filtered out is made whether again
Certain medicine is injected to be judged.
The speed of sorting can reach one animal individual of sorting per second, reach the purpose quickly sorted.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of one embodiment of the present invention；
Fig. 2 is the top view of porous disc in one embodiment of the present invention；
Fig. 3 is the acquisition ROI region algorithm flow chart of one embodiment of the present invention；
Fig. 4 is the morphological operation algorithm flow chart of one embodiment of the present invention.
1- monocrystalline LED；The collimation lenses of 2- first；3- cylindrical mirrors；The collimation lenses of 4- second；One 10 × object lens of 5-；6- is micro-
Measure syringe pump；7- capillaries；2 10 × object lens of 8-；The collimation lenses of 9- the 3rd；10- line array CCDs；11- computers；12- displacement platforms；
13- clips；14- nozzles；15- porous discs.
Embodiment
With reference to specific embodiment, present disclosure is described in further detail：
In order to reach the purpose of the present invention, Fig. 1 shows one embodiment of the present invention.
A kind of small model organism real time imagery and high speed separation system, structure is as shown in figure 1, Part I is illumination system
System, including monocrystalline LED 1, for the light irradiation that monocrystalline LED 1 is sent on f=100mm the first collimation lens 2, the first collimation is saturating
Mirror 2 plays a part of collimated light path, and in f=10mm cylindrical mirror 3, cylindrical mirror 3 can form cylinder light and to spot for the illumination of outgoing
Horse fish carries out fault imaging, and the cylinder illumination being emitted afterwards is on f=200mm the second collimation lens 4, the second collimation lens 4
Collimated light path effect is also functioned to, the light being emitted afterwards enters one 10 × object lens 5 and carries out light beam focusing.
Part II is imaging identifying system, and light beam beats on caliber 3mm capillary 7, have in capillary 7 zebra fish with
Liquid stream flows through.Through capillary 7 light beam by a 2 10 × object lens 8, it is the 3rd accurate to impinge upon f=200mm for subsequent light beam
On straight lens 9, finally zebrafish larvae is imaged by high-resolution linear array CCD 10, imaging is imported into the system of computer 11
In system, zebrafish larvae is identified according to Morphology Algorithm, and generates the movement that instruction carrys out control bit moving stage 12.
Part III is screening system, and zebrafish larvae and liquid are loaded into micro-injection pump 6, micro-injection pump 6 and hair
Tubule 7 is connected, and capillary is clamped on displacement platform 12 with clip 13, and conical nozzle 14, zebra fish are inserted in the bottom of capillary 7
The young and liquid can be flowed into porous on displacement platform 12 by displacement platform 12 after space X, Y, Z axis movement by nozzle 14
In disk 15, the quick sorting to zebrafish larvae is realized.
Lighting part cylindrical mirror 3 wherein in illuminator can form cylinder light, and optical layer is carried out to zebrafish larvae
Cut, then carry out fast imaging by the imaging identifying system of Part II.The displacement platform that Part III liquid fluid system is connected
12 can carry out the movement in X, Y, Z axis by the instruction of the computer 11 of installation control card, so as to by the zebra fish in capillary 7
The young is accurately instilled in porous disc 15.
Fig. 2 is the algorithm flow chart for finding ROI region, first finds out ROI region, then with morphological operation flow pair in Fig. 3
Zebrafish larvae is screened.Related algorithm is embedded into inside control card, so as to the movement of control bit moving stage, carries out spot
The screening of the horse fish young.
It is using the beneficial effect of above-mentioned technical proposal：
The present invention is streaming systems, and when sample flows through imaging capillary along with liquid stream, another side line array CCD is to sample
Carry out real time imagery；The camera of collection image in the present invention is line array CCD, it is possible to achieve high speed imaging.
Lighting part make use of cylindrical mirror, can form mating plate, and sample is illuminated using mating plate, lighting hours compared with
It is short, therefore light injury is smaller to caused by sample.
Fast imaging can be carried out to the small model organism such as zebra fish, and carry out scan picture, according to morphology
Operation, finally can quickly be screened to zebra fish.
On the one hand from being carried out to zebra fish from embryonic development, the zebra fish of different shape is sub-elected, is carried out respectively
Cultivate.On the other hand live body zebrafish larvae can be screened, the live body zebrafish larvae filtered out is made whether again
Certain medicine is injected to be judged.
The speed of sorting can reach one animal individual of sorting per second, reach the purpose quickly sorted.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand present disclosure and be carried out, and it is not intended to limit the scope of the present invention, all according to the present invention
The equivalent change or modification that Spirit Essence is made, it should all cover within the scope of the present invention.
Claims (10)
1. a kind of small model organism real time imagery and high speed separation system, including：
Illuminator, imaging identifying system, screening system,
It is characterized in that：
Illuminator, it is illuminated using mating plate to small model organism；
Identifying system is imaged, it carries out real time imagery to the small model organism in the flowing after illuminating and identifies differentiation；
Screening system, it is instilled small model organism in porous disc using liquid fluid system, realizes the screening of small model organism.
2. a kind of small model organism real time imagery according to claim 1 and high speed separation system, it is characterised in that：Institute
Stating illuminator includes：
Light source, the first collimation lens, cylindrical mirror, the second collimation lens, object lens,
Light source, there is provided illuminating bundle；
First collimation lens, the light beam that light source is sent are collimated；
Cylindrical mirror, the light beam after the first collimation lens is collimated form cylinder light, small model organism are imaged；
Second collimation lens, the light beam that cylindrical mirror projects is collimated again；
First object lens, the light beam projected to the second collimation lens are focused.
3. a kind of small model organism real time imagery according to claim 2 and high speed separation system, it is characterised in that：Institute
Stating imaging identifying system includes：
Micro-injection pump, capillary, image-forming objective lens, lens, line array CCD, computer；
Micro-injection pump, micro-injection pump are connected with capillary, and carrying capacity is provided for the liquid containing small model organism；
Capillary, liquid of the transmission with small model organism；
Second object lens, passed through for the light beam through capillary；
3rd collimation lens, the light beam that the second object lens project is collimated；
Line array CCD, real time imagery is carried out to small model organism, and imaging is imported into computer；
Computer, small model organism is identified according to Morphology Algorithm.
4. a kind of small model organism real time imagery according to claim 3 and high speed separation system, it is characterised in that：Institute
Stating screening system includes：
Displacement platform, conical nozzle, porous disc,
Displacement platform, moved by computer generation instruction come control bit moving stage on space X, Y, Z axis, displacement platform drives capillary to move
It is dynamic；
Conical nozzle, it is arranged on the bottom of capillary and is directed at porous disc；
Porous disc, possess some independent receiving spaces and carry small model organism for accommodating.
5. a kind of small model organism real time imagery according to claim 1 and high speed separation system, it is characterised in that institute
It is zebra fish to state small model organism.
6. a kind of small model organism real time imagery according to claim 2 and high speed separation system, it is characterised in that：Institute
State cylindrical mirror f=10mm.
7. a kind of small model organism real time imagery according to claim 2 and high speed separation system, it is characterised in that：Institute
It is monocrystalline LED to state light source.
8. a kind of small model organism real time imagery according to claim 3 and high speed separation system, it is characterised in that：Institute
State the first collimation lens f=100mm, the second collimation lens f=200mm, the 3rd collimation lens f=200mm.
9. a kind of small model organism real time imagery according to claim 3 and high speed separation system, it is characterised in that：Institute
State the first object lens, the second object lens are 10 × object lens.
10. a kind of small model organism real time imagery according to claim 4 and high speed separation system, it is characterised in that：
The capillary lower end is secured in place in moving stage by clip.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711052704.3A CN107810900A (en) | 2017-11-01 | 2017-11-01 | A kind of small model organism real time imagery and high speed separation system |
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| CN201711052704.3A CN107810900A (en) | 2017-11-01 | 2017-11-01 | A kind of small model organism real time imagery and high speed separation system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113533286A (en) * | 2021-07-20 | 2021-10-22 | 中国科学院苏州生物医学工程技术研究所 | High-flux three-dimensional imaging equipment |
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