CN107805215B - Extraction method of astaxanthin - Google Patents
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- CN107805215B CN107805215B CN201710972211.5A CN201710972211A CN107805215B CN 107805215 B CN107805215 B CN 107805215B CN 201710972211 A CN201710972211 A CN 201710972211A CN 107805215 B CN107805215 B CN 107805215B
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- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 52
- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 52
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 52
- 229940022405 astaxanthin Drugs 0.000 title claims abstract description 52
- 239000001168 astaxanthin Substances 0.000 title claims abstract description 52
- 238000000605 extraction Methods 0.000 title claims abstract description 32
- 239000011347 resin Substances 0.000 claims abstract description 30
- 229920005989 resin Polymers 0.000 claims abstract description 30
- 241000168517 Haematococcus lacustris Species 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000000227 grinding Methods 0.000 claims abstract description 12
- 108010059892 Cellulase Proteins 0.000 claims abstract description 11
- 229940106157 cellulase Drugs 0.000 claims abstract description 11
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 10
- 108010093305 exopolygalacturonase Proteins 0.000 claims abstract description 10
- 238000000746 purification Methods 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims description 36
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- 239000002244 precipitate Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 238000006911 enzymatic reaction Methods 0.000 claims description 11
- 239000011780 sodium chloride Substances 0.000 claims description 11
- 238000004108 freeze drying Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- NIQQIJXGUZVEBB-UHFFFAOYSA-N methanol;propan-2-one Chemical compound OC.CC(C)=O NIQQIJXGUZVEBB-UHFFFAOYSA-N 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 230000001066 destructive effect Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000223252 Rhodotorula Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 238000012733 comparative method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- -1 light Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides an extraction method of astaxanthin, which mainly comprises the technical means of low-temperature grinding, enzymatic low-temperature wall breaking, organic solvent multi-stage extraction, combined macroporous resin purification and the like, wherein the low-temperature grinding method is firstly utilized to carry out primary wall breaking on haematococcus pluvialis, then cellulase and pectinase are combined to carry out secondary wall breaking, the temperature during secondary wall breaking is 0-4 ℃, the conventional wall breaking temperature selection thought is broken through, the destructive effect of high temperature on effective components of the haematococcus pluvialis is avoided, the full wall breaking of cells is realized, and a good foundation is provided for subsequent astaxanthin extraction. Through a multi-stage extraction mode, the conditions are mild, the astaxanthin cannot be damaged, and meanwhile, the combined macroporous resin is adopted for purification, so that the extraction rate and the purity of the astaxanthin are greatly improved, the utilization value of haematococcus pluvialis is improved, the economic benefit is increased, and a solid foundation is laid for industrial production.
Description
Technical Field
The invention relates to the technical field of extraction of natural products, in particular to an extraction method of astaxanthin.
Background
Astaxanthin, also called astaxanthin or astaxanthin, is a carotenoid, a strong antioxidant, exists in shrimps, crabs, algae and yeasts, and is known to be haematococcus pluvialis and rhodotorula farinosa, which contain mainly esterified astaxanthin and are stored in vivo as more stable non-oxidized astaxanthin, so that astaxanthin is known to be the best organism for producing astaxanthin in nature, because the natural astaxanthin with higher content is currently known.
Astaxanthin is a long-chain unsaturated double-bond structure system, so that the astaxanthin is unstable in property, easy to isomerize and degrade and sensitive to heat, oxygen, light, acid, alkali and the like. At present, the extraction rate and purity of astaxanthin are low, and the production cost is high, so that a more comprehensive extraction method needs to be developed, the extraction rate and purity of astaxanthin are improved, and the economic benefit of astaxanthin is increased.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an extraction method of astaxanthin.
The technical scheme adopted by the invention is as follows:
a method for extracting astaxanthin comprises the following steps:
s1: taking 3-5 g of haematococcus pluvialis powder, grinding at low temperature, adding 200-300 mL of 0.3-0.6 mg/mL cellulase and 0.1-0.3 mg/mL pectinase for wall breaking, performing enzyme reaction at the wall breaking temperature of 0-4 ℃ for 45-60 min and the pH of the enzyme reaction of 3.5-5.0, adding 0.5-2 g/mL sodium chloride solution, mixing for 5-10 min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, and mixing the precipitate according to the material-liquid ratio of 1: 2-3, adding a methanol solution containing 2-3% of sodium hydroxide, reacting for 1-2 h, adding edible oil-acetone-ethyl acetate for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solutions with different volume ratios, separating, and mixing to obtain filter residue Y and an extracting solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and purifying by selecting a combined macroporous resin formed by mixing three macroporous resin materials to obtain the astaxanthin.
Preferably, the addition amount of the sodium chloride in the step S1 is 20-50 ml, and the centrifugation parameters are as follows: centrifuging at 2000-3000 rpm for 5-10 min.
Preferably, in the step S2, the volume ratio of the edible oil to the acetone to the ethyl acetate is 50-52: 25-29: 19-25, and the volume ratio of the acetone to the methanol is 99:1, 75-88: 12-25, 50-60: 40-50 and 0:100 respectively.
Preferably, in the step S3, the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 respectively.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the method, firstly, the haematococcus pluvialis is subjected to primary wall breaking by using a low-temperature grinding method, and then secondary wall breaking is performed by combining cellulase and pectinase, so that the full wall breaking of cells is realized, the temperature during secondary wall breaking is 0-4 ℃, the conventional wall breaking temperature selection idea is broken through, the damage effect of high temperature on the effective components of the haematococcus pluvialis is avoided, and a good foundation is provided for the subsequent extraction of astaxanthin.
2. The method has the advantages that the conditions are mild, the astaxanthin cannot be damaged by a multi-stage extraction mode, and the combined macroporous resin is adopted for purification, so that the extraction rate and the purity of the astaxanthin are greatly improved, the utilization value of haematococcus pluvialis is improved, the economic benefit is increased, and a solid foundation is laid for industrial production.
Detailed Description
The invention will be further described with reference to specific examples, the advantages and features of which will become clearer from the following description, but the scope of protection of the invention is not limited to the following examples.
Example 1:
a method for extracting astaxanthin comprises the following steps:
s1: grinding 3g of haematococcus pluvialis powder at low temperature, adding 300mL of 0.4mg/mL of cellulase and 0.1mg/mL of pectinase for wall breaking, wherein the wall breaking temperature is 0 ℃, the wall breaking time is 60min, the enzymatic reaction pH is 4, adding 50mL of 0.7g/mL of sodium chloride solution, mixing for 10min, centrifuging at 2000rpm for 5min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 3% of sodium hydroxide according to the material-liquid ratio of 1: 2, reacting for 1h, adding edible oil-acetone-ethyl acetate (the volume ratio is 52: 29: 19) for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solution at volume ratio of 99:1, 88:12, 60:40, and 0:100, separating, and mixing to obtain filter residue Y and extractive solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting and purifying the combined macroporous resin formed by mixing three macroporous resin materials, wherein the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 to obtain the astaxanthin.
Example 2:
a method for extracting astaxanthin comprises the following steps:
s1: grinding 5g of Haematococcus pluvialis powder at low temperature, adding 200mL of 0.3mg/mL cellulase and 0.1mg/mL pectinase for wall breaking, at the wall breaking temperature of 4 ℃, for 45min, for enzyme reaction pH of 3.5, adding 50mL of 2g/mL sodium chloride solution, mixing for 10min, centrifuging at 2000rpm for 10min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 2% of sodium hydroxide according to the material-liquid ratio of 1: 3, reacting for 2h, adding edible oil-acetone-ethyl acetate (the volume ratio is 50: 25) for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solution at volume ratio of 99:1, 75: 25, 60:40, 0:100, separating, and mixing to obtain filter residue Y and extractive solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting and purifying the combined macroporous resin formed by mixing three macroporous resin materials, wherein the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 to obtain the astaxanthin.
Example 3:
a method for extracting astaxanthin comprises the following steps:
s1: grinding 3g of haematococcus pluvialis powder at low temperature, adding 300mL of 0.6mg/mL of cellulase and 0.3mg/mL of pectinase for wall breaking, wherein the wall breaking temperature is 0 ℃, the wall breaking time is 45min, the enzymatic reaction pH is 5.0, adding 20mL of 0.5g/mL of sodium chloride solution, mixing for 5min, centrifuging at 3000rpm for 5min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 3% of sodium hydroxide according to the material-liquid ratio of 1: 3, reacting for 1h, adding edible oil-acetone-ethyl acetate (the volume ratio is 52: 29: 19) for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solution at volume ratio of 99:1, 88:12, 50: 50, 0:100, separating, and mixing to obtain filter residue Y and extractive solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting and purifying the combined macroporous resin formed by mixing three macroporous resin materials, wherein the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 to obtain the astaxanthin.
Example 4:
a method for extracting astaxanthin comprises the following steps:
s1: grinding 4g of haematococcus pluvialis powder at low temperature, adding 250mL of 0.5mg/mL cellulase and 0.2mg/mL pectinase for wall breaking, wherein the wall breaking temperature is 2 ℃, the wall breaking time is 50min, the enzymatic reaction pH is 4.5, adding 25mL of 1.5g/mL sodium chloride solution, mixing for 5min, centrifuging at 2000rpm for 5min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 3% of sodium hydroxide according to the material-liquid ratio of 1: 3, reacting for 1h, adding edible oil-acetone-ethyl acetate (the volume ratio is 52: 29: 19) for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solution at volume ratio of 99:1, 80: 20, 55: 45, and 0:100, separating, and mixing to obtain filter residue Y and extractive solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting and purifying the combined macroporous resin formed by mixing three macroporous resin materials, wherein the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 to obtain the astaxanthin.
Comparative example 1:
a method for extracting astaxanthin comprises the following steps:
s1: grinding 3g of haematococcus pluvialis powder at low temperature, adding 100mL of 0.1mg/mL cellulase and 0.1mg/mL pectinase for wall breaking, wherein the wall breaking temperature is 0 ℃, the wall breaking time is 60min, the enzymatic reaction pH is 3.5, adding 20mL of 2g/mL sodium chloride solution, mixing for 10min, centrifuging at 3000rpm for 5min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 1% of sodium hydroxide according to the material-liquid ratio of 1: 2, reacting for 1h, adding edible oil-acetone-ethyl acetate (the volume ratio is 30: 40: 30) for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solution at volume ratio of 99:1, 60:40, 30: 70, and 0:100, separating, and mixing to obtain filter residue Y and extractive solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting and purifying the combined macroporous resin formed by mixing three macroporous resin materials, wherein the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 to obtain the astaxanthin.
Comparative example 2:
the extraction method of astaxanthin is characterized by comprising the following steps:
s1: grinding 3g of haematococcus pluvialis powder at low temperature, adding 200mL of 1mg/mL of cellulase and 0.5mg/mL of pectinase for wall breaking, wherein the wall breaking temperature is 0 ℃, the wall breaking time is 60min, the enzyme reaction pH is 3.5, adding 20mL of 2g/mL of sodium chloride solution, mixing for 10min, centrifuging at 3000rpm for 5min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 5% of sodium hydroxide according to the material-liquid ratio of 1: 2, reacting for 1h, adding edible oil-acetone-ethyl acetate (the volume ratio is 60: 35: 5) for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solutions with volume ratios of 99:1, 95: 5, 70: 30 and 10: 90, separating, and mixing to obtain filter residue Y and extractive solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting and purifying the combined macroporous resin formed by mixing three macroporous resin materials, wherein the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 to obtain the astaxanthin.
Comparative example 3:
a method for extracting astaxanthin comprises the following steps:
s1: same as example 1;
s2: same as in example 1;
s3: and mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selectively purifying by using AB-8 type macroporous resin to obtain the astaxanthin.
Comparative example 4:
a method for extracting astaxanthin comprises the following steps:
s1: taking 3g of haematococcus pluvialis powder, adding 200mL of 0.4mg/mL of cellulase for wall breaking, wherein the wall breaking temperature is 0 ℃, the wall breaking time is 60min, the enzymatic reaction pH is 4, adding 50mL of 0.7g/mL sodium chloride solution, mixing for 10min, centrifuging at 2000rpm for 5min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, adding a methanol solution containing 3% sodium hydroxide according to the material-liquid ratio of 1: 2, reacting for 1h, adding edible oil-acetone-ethyl acetate (the volume ratio is 52: 29: 19) for extraction, and separating to obtain an extracting solution;
s3: freeze drying the extracted liquid, and purifying with three kinds of mixed macroporous resin material, including XDA-1, XDA-6 and XAD-16, to obtain astaxanthin.
Comparative example 5:
a method for extracting astaxanthin comprises the following steps:
s1: same as example 1 except that the wall breaking temperature was 40 ℃;
s2: same as example 1;
s3: same as in example 1.
Experimental example:
the extraction rate and purity of astaxanthin were measured in examples and comparative examples, respectively. The results are shown in Table 1.
Table 1: measurement results
According to experimental results, the extraction rate of the astaxanthin obtained by the method is as high as 99.1%, the purity of the astaxanthin is as high as 99.8%, and the extraction rate and the purity of the astaxanthin are far higher than those of the astaxanthin obtained by the comparative example. It can be seen that the invention combines the technical means of low-temperature grinding, enzymatic low-temperature wall breaking, organic solvent multi-stage extraction, combined macroporous resin purification and the like, and obtains the technical effect which is obviously superior to that of the comparative method.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (3)
1. The extraction method of astaxanthin is characterized by comprising the following steps:
s1: taking 3-5 g of haematococcus pluvialis powder, grinding at low temperature, adding 200-300 mL of 0.3-0.6 mg/mL cellulase and 0.1-0.3 mg/mL pectinase for wall breaking, performing enzyme reaction at the wall breaking temperature of 0-4 ℃, for 45-60 min and at the enzyme reaction pH of 3.5-5.0, adding 0.5-2 g/mL sodium chloride solution, mixing for 5-10 min, and separating supernatant and precipitate;
s2: taking the precipitate in the step S1, and mixing the precipitate according to the material-liquid ratio of 1: 2-3, adding a methanol solution containing 2-3% of sodium hydroxide, reacting for 1-2 h, adding edible oil-acetone-ethyl acetate for extraction, and separating to obtain an extracting solution a and filter residue X; sequentially extracting the filter residue X with acetone-methanol solutions with different volume ratios, separating, and mixing to obtain filter residue Y and an extracting solution b; extracting the filter residue Y with ethanol, and separating to obtain an extracting solution c;
s3: mixing the extracting solution a, the extracting solution b and the extracting solution c, freeze-drying, and selecting a combined macroporous resin formed by mixing three macroporous resin materials for purification to obtain the astaxanthin; the three macroporous resin materials are XDA-1, XDA-6 and XAD-16 respectively.
2. The method for extracting astaxanthin according to claim 1, wherein the amount of sodium chloride added in step S1 is 20-50 ml, and the centrifugation parameters are as follows: centrifuging at 2000-3000 rpm for 5-10 min.
3. The extraction method of astaxanthin according to claim 1, wherein the volume ratio of edible oil-acetone-ethyl acetate in step S2 is 50-52: 25-29: 19-25, wherein the volume ratio of acetone-methanol is 99:1, 75-88: 12-25, 50-60: 40-50 and 0:100 respectively.
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| CN108866144B (en) * | 2018-07-24 | 2020-11-17 | 杭州园泰生物科技有限公司 | Method for preparing and purifying astaxanthin |
| CN109480285A (en) * | 2018-12-10 | 2019-03-19 | 丽江程海湖天然螺旋藻生产基地有限公司 | A kind of haematococcus pluvialis soft capsule |
| CN109645295A (en) * | 2019-01-17 | 2019-04-19 | 云南龙布瑞生物科技有限公司 | A kind of Astaxanthin In Haematococcus Pluvialis solid beverage maintaining cardiovascular system health |
| CN111793014B (en) | 2020-07-27 | 2021-07-13 | 中国海洋大学 | Method for preparing water-soluble astaxanthin and astaxanthin aqueous solution prepared by method |
| CN113249291A (en) * | 2021-07-01 | 2021-08-13 | 海南三元星生物科技股份有限公司 | Method for inducing haematococcus pluvialis cell type conversion and accumulating astaxanthin by using seawater |
| CN116622797A (en) * | 2023-04-03 | 2023-08-22 | 广州优卡思农业技术有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
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| CN103880726A (en) * | 2014-03-14 | 2014-06-25 | 山东省农业科学院农产品研究所 | Method for extracting astaxanthin in haematococcus pluvialis by adopting enzymolysis method and grinding method through organic solvent |
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| CN103880726A (en) * | 2014-03-14 | 2014-06-25 | 山东省农业科学院农产品研究所 | Method for extracting astaxanthin in haematococcus pluvialis by adopting enzymolysis method and grinding method through organic solvent |
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| Title |
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| 雨生红球藻中虾青素提取方法的比较研究;王灵昭;《食品研究与开发》;20080731;第29卷(第7期);第16-18页 * |
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