CN107789666A - A kind of inwall micro-patterning small-caliber artificial blood vessel - Google Patents
A kind of inwall micro-patterning small-caliber artificial blood vessel Download PDFInfo
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- CN107789666A CN107789666A CN201610768283.3A CN201610768283A CN107789666A CN 107789666 A CN107789666 A CN 107789666A CN 201610768283 A CN201610768283 A CN 201610768283A CN 107789666 A CN107789666 A CN 107789666A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
Abstract
The present invention relates to technical field of biological materials, there is provided a kind of inwall micro-patterning small-caliber artificial blood vessel.The small-caliber artificial blood vessel has double-layer structure.Outer layer is electrostatic spinning microfibril layer, improves the mechanical property of small-caliber artificial blood vessel, keeps the stability of its tubular structure.Internal layer is the coat with micropatterning, adhesion beneficial to endothelial cell, sprawls and grows, and endothelial cell skeleton can be promoted to extend and guiding endothelial cell oriented growth, ensures the normal performance of cell function.And current product or the mechanical property of the small-caliber artificial blood vessel of research are poor, it is impossible to fully meet the requirement of clinic, and the normal performance of the oriented growth and cell function of endothelial cell can not be promoted.The inwall micro-patterning small-caliber artificial blood vessel of the present invention has good mechanical property and biology performance, suitable for damage or the reparation and displacement of lesion small-caliber vascular tissue.
Description
Technical field
The present invention relates to technical field of biological materials, more particularly to a kind of micro-patterning small-caliber artificial blood vessel.
Background technology
Angiocardiopathy turns into the first killer for threatening human health.Many angiocardiopathies generation all with blood vessel
It is narrow or inaccessible closely related.The proper flow of the narrow or inaccessible obstruction blood of blood vessel, causes tissue because of nutrition supply deficiency
And damage.Common disease has coronary heart disease, cranial vascular disease, Peripheral atherosclerosis and DVT etc..Blood vessel weight
Build be this kind of disease treatment a kind of important method, in occupation of highly important status in Clinical Surgery.By the end of current, only
The U.S. just has more than 500,000 patient every year to be needed to carry out vascular operation, and wherein Most patients are required for suitably
Blood vessel graft.
At present, the small-caliber vascular graft clinically applied is mainly autologous vein, saphena and arteria thoracica interna quilt
It is known as the golden standard of small-caliber vascular graft.Although autologous vein represents the gold mark of small-caliber vascular graft
Standard, but the patency rate of autogenous vessel graft thing is still less desirable.In coronary artery He in Gu popliteal artery bypass surgeries, from
Long-term patency rate of the body saphena after transplanting 10 years is only 50%.Autologous vein limited source is additionally, since, and supplies area's sacrifice
Larger, its application clinically is also extremely limited.
Artificial blood vessel is considered as a kind of blood vessel graft of alternative autologous vein.Compared with autologous vein, artificial blood
Pipe has the advantages that abundance, convenient material drawing, and its research is widely paid close attention to.Terylene and expanded polytetrafluoroethylsealing are current
Most commonly used artificial blood vessel, this kind of polymer artificial blood vessel have been successfully applied to clinic in terms of the big-and-middle artery of human body is substituted
And achieve satisfied effect.However, small-bore (diameter prepared by this kind of polymeric material<6mm) artificial blood vessel is small in replacement
Almost all failure in terms of calibre, it is ground acute embolic and endometrial hyperplasia easily occurs after being implanted into vivo the problems such as
Study carefully and show that expanded polytetrafluoroethylsealing artificial blood vessel is used for coronary artery bypass surgery, postoperative 1 year its patency rate is only 60%, remote low
In the patency rate of saphena 95%, the patency rate of postoperative 2 years expanded polytetrafluoroethylsealing artificial blood vessels is only 32%, now saphena
Patency rate remain at more than 90%.In above-knee Gu popliteal artery bypass surgery, postoperative 5 years expanded polytetrafluoroethylsealing artificial blood
The patency rate of pipe is 59%, far below the patency rate of saphena 78%.Moreover, vascular endothelial cell is in above-mentioned polymer artificial blood
Inside pipe wall is not easy to adhere to, sprawl, grow and breed, and its biocompatibility need to be improved.
In recent years, acute thrombus and endometrial hyperplasia can be avoided by developing, and possess excellent mechanical performances and biology performance
Small-caliber artificial blood vessel become a study hotspot both domestic and external.Researcher uses fibroin albumen, collagen, fine even egg
In vain, the natural macromolecular materials such as cellular matrix are gone to prepare small-caliber artificial blood vessel, this kind of small-caliber artificial blood vessel has good
Biocompatibility, but Mechanical test results show that their mechanical property is poor, it is impossible to meet clinically to small-bore artificial
The mechanical property requirements of blood vessel.And synthesize polymeric biomaterial because with good mechanical property and biocompatibility and by
It is widely used in preparing small-caliber artificial blood vessel, researcher is natural using one or more synthesis high polymer materials, or joint
High polymer material prepares small-caliber artificial blood vessel, and research has shown that this kind of small-caliber artificial blood vessel has good mechanical property and life
Thing compatibility.However, such small-caliber artificial blood vessel there is also it is certain the problem of, it is small-bore artificial that endothelial cell is seeded in these
Blood vessel shows as random mixed and disorderly growth, with native blood vessels endothelial cell along direction of flow of blood aligned growth difference compared with
Greatly, inner skin cell function is suppressed.
The biomaterial surface modification that develops into of micro- patterning technique provides new technology.The micropatterning of material surface
Interaction between controllable cell and material, promotes adhesion, growth, propagation and the differentiation of cell, and can influence cell
The expression of the direction of growth, the arranged distribution of cytoskeleton and cell function gene.Therefore, micro- patterning technique is relied on to prepare inwall micro-
The small-caliber artificial blood vessel of patterning is expected to solve the above problems.
The content of the invention
Small-caliber artificial blood vessel mechanical property for solution prior art preparation is poor and endothelial cell can not be guided to be orientated
The problem of growth is so as to suppress inner skin cell function, the present invention, which provides one kind, can guide endothelial cell oriented growth, and have
The inwall micro-patterning small-caliber artificial blood vessel of excellent mechanical performances and biocompatibility.Its technical solution is：
Inwall micro-patterning small-caliber artificial blood vessel is made up of electrostatic spinning microfibril layer and micro- pattern coat.Its
In, micro- pattern coat forms the internal layer of small-caliber artificial blood vessel, and electrostatic spinning microfibril layer wraps up micro- pattern coat, is formed
The outer layer of small-caliber artificial blood vessel.First, by micro- pattern polydimethylsiloxanefilm film volume in metal shaft, metal shaft is equal in advance
One layer of polyethylene glycol soluble in water of even coating, then coating liquid is coated in micro- pattern polydimethylsiloxanefilm film surface, apply
Covering liquid can mark micro- pattern to coat after drying solidification from polydimethylsiloxanefilm film, form micro- pattern coating
Layer, coating liquid are the blended liquid of silk fibroin protein solution and glycerine, and wherein glycerine can play a part of modified fibroin albumen so that
The coat arrived is more flexible.Electrostatic spinning microfibril layer is prepared by electrostatic spinning apparatus, and spinning solution is fibroin albumen/PLA
The hexafluoroisopropanol solution of caprolactone blending, collection device are rotation circular shaft collector at a high speed.Electrostatic spinning layer is as outer layer knot
Structure, the tubular structure of small-caliber artificial blood vessel can be consolidated, after natural macromolecular material fibroin albumen adds PLA caprolactone
The mechanical property of electrostatic spinning microfibril layer can be significantly increased, improves inwall micro-patterning small-caliber artificial blood vessel each side
Mechanical property.
Due to there is micropatterning, energy using technical scheme, small-caliber artificial blood vessel inwall of the invention
Enough guide endothelial cell oriented growth, can effectively solve endothelial cell inoculated and cultured small-caliber artificial blood vessel function of surface by
To the problems such as suppression.Meanwhile the performance of two kinds of materials is combined using fibroin albumen and PLA caprolactone co-blended spinning liquid, can
Effectively solves the problems such as small-caliber artificial blood vessel mechanical property deficiency and poor biocompatibility.The inwall micro-patterning of the present invention
Small-caliber artificial blood vessel can be widely applied to biologic medical field.
Brief description of the drawings
Fig. 1 is the electrostatic spinning schematic diagram that the embodiment of the present invention 1 prepares micro- pattern small-caliber artificial blood vessel；
Fig. 2 is micro- pattern small-caliber artificial blood vessel exterior appearance figure prepared by implementation column 1 of the present invention；
Fig. 3 is the ESEM that micro- pattern small-caliber artificial blood vessel section prepared by the embodiment of the present invention 1 amplifies 500 times
Figure；
Fig. 4 is the scanning electricity that micro- pattern small-caliber artificial blood vessel inner surface prepared by the embodiment of the present invention 1 amplifies 1000 times
Mirror figure；
Fig. 5 cultivates the endothelial cell immunotoxin fluorescence dye in micro- pattern small-caliber artificial blood vessel inwall for the embodiment of the present invention 3
Chromatic graph；
Embodiment
Specifically in conjunction with the embodiments, the present invention is further elaborated.These embodiments are merely to illustrate the spy of the present invention
Advantage of seeking peace rather than limitation the scope of the present invention.
The invention provides a kind of inwall micro-patterning small-caliber artificial blood vessel, specific implementation step are as follows：
One layer of photoresist of uniform fold on the glass sheet, micro- pattern is prepared on photoresist surface using ultraviolet photolithographic machine, is obtained
To micro- patterned photo glue, the preferably micro- pattern of parallel stripes, width of fringe and spacing are 20 μm~30 μm, are highly 2-5 μm.
By dimethyl silicone polymer uniform fold on above-mentioned micro- patterned photo glue surface, being placed in heating plate after solidifying will be poly-
Dimethyl siloxane is taken off, obtains micro- pattern polydimethylsiloxanefilm film, and micro- pattern polydimethylsiloxanefilm film thickness is excellent
Elect 10 μm~50 μm as.
The polyethylene glycol of melting is coated uniformly in metal shaft, by above-mentioned micro- pattern polydimethylsiloxanes after cooled and solidified
For alkane film roll on the metal shaft surface, obtaining volume has a metal shaft of micro- pattern polydimethylsiloxanefilm film, and metal shaft diameter is excellent
Elect 1~6mm as.
Silkworm raw silk is subjected to degumming, dissolving, dialysis successively, obtains silk fibroin water solution.
The silk fibroin water solution is concentrated with polyethylene glycol, obtains fibroin albumen concentrated solution, fibroin albumen is dense
Solution is preferably 15%~25%.
The silk fibroin water solution is freeze-dried, obtains regenerated silk fibroin sponge；
Described fibroin albumen concentrated solution is mixed with glycerine, the quality of glycerine and fibroin albumen in fibroin albumen concentrated solution
Than being preferably 0.5~2:1, fully vibration obtains coating liquid after mixing, and coating liquid is had into the poly- diformazan of micro- pattern coated on above-mentioned volume
In the metal shaft of radical siloxane film, and at the uniform velocity rotating metallic axle ensures the micro- pattern dimethyl silicone polymer of coating liquid uniform fold
Film；
The metal shaft covered with coating liquid is arranged on electrostatic spinning at a high speed on rotation circular shaft collector, with fibroin egg
White and PLA caprolactone hexafluoroisopropanol solution is spinning solution, and spinning solution total concentration is preferably 6%~15%, more preferably
9%~12%, the wherein ratio of fibroin albumen and PLA caprolactone is preferably 0.5~3:1, more preferably 2~3:1.Utilize
Electrostatic spinning technique forms one layer of uniform electrostatic spinning microfibril layer in coat outer surface, and electrospinning parameters are preferably：
Voltage is 10kV~20kV, and it is 0.5mL/h~2mL/h that spinning solution, which injects speed, and the reception distance of syringe needle and metal shaft is 10cm
~20cm, it is 500r/min~4000r/min to rotate circular shaft collector rotating speed at a high speed, and electrostatic spinning total time is 3h~5h.It is quiet
It is soaked in after the completion of Electrospun in absolute ethyl alcohol, modified fibroin albumen, above-mentioned metal shaft is taken out after immersion treatment, after dries
It is soaked in again in distilled water, after the polyethylene glycol on metal shaft surface is completely dissolved, extracts metal shaft out, obtains inwall and have poly- two
The small-caliber artificial blood vessel of methylsiloxane film, gently peels off polydimethylsiloxanefilm film, and it is small to obtain inwall micro-patterning
Bore artificial blood vessel, micro- pattern small-caliber artificial blood vessel wall thickness are preferably 0.3mm~0.4mm, and coat thickness is preferably
0.02mm~0.05mm, electrostatic spinning thickness degree is preferably 0.25mm~0.35mm.
For a further understanding of the present invention, with reference to embodiment to the small-bore people of inwall micro-patterning provided by the invention
Hematopoiesis pipe is described in detail.
Example 1
1. use sol evenning machine that on the glass sheet, the positive photoresist uniform folds of AR-P 3250 are used into purple after 95 DEG C of solidifications
It is 20 μm that outer litho machine, which prepares width of fringe and spacing, and depth is 2 μm of the micro- patterned photo glue of parallel stripes；
2. dimethyl silicone polymer uniform fold is placed in heating plate on above-mentioned micro- patterned photo glue surface using sol evenning machine
Upper 95 DEG C of solidifications 2h, is carefully taken off the film on surface using tweezers, produces micro- pattern polydimethylsiloxanefilm film after solidification；
3. 20g silkworms raw silk is boiled into 30min in the sodium carbonate deionized water solution that 2L concentration is 0.02mol/L to carry out
Degumming process, the silk after boiling is cleaned 6 times with deionized water, be placed in 37 DEG C of baking ovens and dry after pulling loose, obtain degumming silkworm
Silk；
4. boiled silk is pressed into 27g：100mL ratio is dissolved in 9.3mol/L lithium bromide water solutions, in 60 DEG C of baking ovens
Stirring and dissolving 4h obtains fibroin albumen salting liquid；
5. by the fibroin albumen saline infusion in molecular cut off be 3.5kDa bag filter in after be soaked in from
In sub- water, deionized water is changed every 2h, persistently dialyses 4 days, obtains silk fibroin water solution, by the fibroin albumen of part gained
Aqueous solution freeze-drying obtains regenerated silk fibroin sponge, and remaining silk fibroin water solution is concentrated with polyethylene glycol, obtained dense
Spend the fibroin albumen concentrated solution for 20%；
6. the fibroin albumen concentrated solution that the concentration is 20% is pressed into Solute mass than 1 with glycerine:1 blending, fully vibration
Bubble is removed in centrifugation after mixing；Described micro- pattern polydimethylsiloxanefilm film is entirely rolled up and is scribbling the 3mm of polyethylene glycol
In metal shaft, then metal shaft is arranged at a high speed on rotation circular shaft collector, by fibroin albumen under 500r/min rotating speed
Micro- pattern polydimethylsiloxanefilm film surface is coated uniformly on the blended liquid of glycerine, forms micro- pattern coat；
7. it is the electrostatic spinning schematic diagram that micro- pattern small-caliber artificial blood vessel is prepared in the present embodiment as shown in Figure 1, by institute
The regenerated silk fibroin sponge stated and PLA caprolactone in mass ratio 3:1, which is configured to the hexafluoroisopropanol that total concentration is 12%, spins
Silk liquid, electrostatic spinning is carried out in micro- pattern coating layer surface, penetrating pump fltting speed during electrostatic spinning is set to 1mL/L, voltage
It is set to 15kV, syringe needle is set to 15cm with metal shaft distance, and the rotating speed of collector is adjusted to 2000r/min, and electrostatic spinning total time is
4h, electrostatic spinning microfibril layer is formed in micro- pattern coat outer surface after the completion of electrostatic spinning；It will carry small-bore artificial
The metal shaft of blood vessel handles 30min modified fibroin albumen with absolute ethyl alcohol, and drying is taken out after immersion, and deionization is soaked in after drying
Water, after the polyethylene glycol on metal shaft surface is completely dissolved, extract metal shaft out, obtain inwall and have polydimethylsiloxanefilm film
Small-caliber artificial blood vessel；
8. small-caliber artificial blood vessel of the described inwall with polydimethylsiloxanefilm film is gently peeled off into poly dimethyl
Siloxane film, 48h in deionized water is soaked in, removes the glycerine to dissociate in micro- pattern coat, it is micro- to produce inwall after drying
Pattern small-caliber artificial blood vessel.
It is illustrated in figure 2 the exterior appearance figure of inwall micro-patterning small-caliber artificial blood vessel made from the present embodiment, (A) figure
It is about 3.3cm to show the small-caliber artificial blood vessel length, and (B) figure shows that the small-caliber artificial blood vessel internal diameter is about 3.5mm.
The section surface sweeping electron microscope of inwall micro-patterning small-caliber artificial blood vessel made from the present embodiment is illustrated in figure 3,
As seen from the figure, the small-caliber artificial blood vessel is made up of the micro- pattern coat of inwall and outer wall electrostatic spinning microfibril layer.
The surface sweeping electron microscope of small-caliber artificial blood vessel inner surface made from the present embodiment is illustrated in figure 4, as seen from the figure, should
Micro- pattern of small-caliber artificial blood vessel inwall is regular parallel stripe patterns.
Embodiment 2
1. the inwall micro-patterning small-caliber artificial blood vessel prepared in embodiment 1 is soaked in phosphate buffer, fill
Divide moistening, the small-caliber artificial blood vessel moistened；
2. the small-caliber artificial blood vessel of the moistening to be cut to the test specimen of 3cm length, the omnipotent material of Japanese Shimadzu is used
Expect that testing machine carries out axial tension mechanics test, selected range is 100N load transducer, and rate of extension is set to 5mm/min；
3. the small-caliber artificial blood vessel of the moistening to be cut to the test specimen of 6mm length, above-mentioned material testing machine is used
Radial drawing mechanical test is carried out, selected range is 100N load transducer, and rate of extension is set to 3mm/min；
4. the small-caliber artificial blood vessel of the moistening to be cut to the test specimen of 2cm length, above-mentioned material testing machine is used
Suture strength mechanical test is carried out with 6-0 polyglycolic acids band pin operation suture thread, selected range is 100N load transducer, is drawn
Stretch speed and be set to 3mm/min；
5. the small-caliber artificial blood vessel of the moistening to be cut to the test specimen of 3cm length, explosion compression testing device is used
Carry out burst pressure test；
Arranged 6. mechanical test data are preserved, draw items of the inwall micro-patterning small-caliber artificial blood vessel in hygrometric state
Mechanical test result, its axial tensile strength are 2.48MPa, breaking strain 33.06%, modulus of elasticity 11.61MPa；Radially
Tensile strength is 2.70MPa, modulus of elasticity 11.89MPa；Suture strength is 1.86N；Burst pressure is 470.82mmHg；Meet
Clinically to the mechanical property requirements of small-caliber artificial blood vessel.
Example 3
1. the inwall micro-patterning small-caliber artificial blood vessel prepared in example 1 to be cut to the test specimen of 1cm length, soak
In phosphate buffer, sterilization treatment is carried out using high-pressure sterilizing pot, obtains sterility test sample；
2. by the sterility test sample and cell inoculation used in utensil be placed in super-clean bench, using ultraviolet sterilization 0.5h with
On, sterility test sample is moved in 24 orifice plates, and 1x10 is inoculated with test specimen inwall after sterility test sample drying5In individual
Chrotoplast, culture 2h in CO2gas incubator is placed in after the completion of inoculation makes cell adherence, then adds the training of 0.8mL endothelial cells
Base is supported, is put into CO2gas incubator culture 3 days；
3. the small-caliber artificial blood vessel after culture is carried out into immunofluorescence dyeing, the ghost of marked by fluorescein isothiocyanate is used
Cyclic peptide dyes to cytoskeleton F-actin, then reuses 4,6- diamidines -2-phenylindone and redyes cell
Core, it is placed under laser confocal microscope and takes pictures, assesses the biology performance of inwall micro-patterning small-caliber artificial blood vessel.
It is illustrated in figure 5 the endothelial cell immunotoxin fluorescence cultivated in the present embodiment in micro- pattern small-caliber artificial blood vessel inwall
Colored graph, as seen from the figure, the small-caliber artificial blood vessel have good biocompatibility, the endothelial cell shape of wall growth in the blood vessels
State is good, moreover, the micropatterning of inwall can guide the oriented growth of endothelial cell, makes endothelial cell along parallel stripes
Growth, contributes to the normal performance of inner skin cell function.
From the above results, the micro- pattern small-caliber artificial blood vessel of inwall that prepared by the present invention has good mechanical property
Can, meet clinically to the mechanical property requirements of small-caliber artificial blood vessel.Meanwhile the small-caliber artificial blood vessel has good life
Thing compatibility, the micropatterning of inwall can guide the oriented growth of endothelial cell, promote the normal hair of inner skin cell function
Wave, the small-caliber vascular suitable for repairing and substituting damage or lesion.
The explanation of above example is only intended to help the method and its core concept for understanding the present invention.It should be pointed out that this
Invention is not limited to above-mentioned embodiment, in the case of without departing substantially from the substantive content of the present invention, the common skill of the art
Art personnel can also carry out some deformations, modification and improve to the present invention, and these deformations, modification and improvement also fall into power of the present invention
In the protection domain that profit requires.
Claims (7)
1. a kind of inwall micro-patterning small-caliber artificial blood vessel, it is characterised in that electrostatic spinning microfibril layer wraps up micro- pattern and applied
The small-caliber artificial blood vessel that coating is formed.
2. inwall micro-patterning small-caliber artificial blood vessel according to claim 1, it is characterised in that internal diameter is in 1mm-6mm
Between.
3. inwall micro-patterning small-caliber artificial blood vessel according to claim 1, it is characterised in that inwall has micro- pattern
Structure, micro- pattern are parallel stripes, circular groove or projection, square groove or projection, triangular groove or projection, donut
The raised or compound pattern of groove, helical groove or projection and above-mentioned a variety of micro- patterns, and micro- pattern accuracy is controllable, up to micron
Or nanoscale.
4. the inwall micro-patterning small-caliber artificial blood vessel according to claims, the micro- pattern coat of its inwall is used to be applied
The solute of covering liquid can be fibroin albumen, chitosan, collagen, polycaprolactone, polyurethane, polyglycolic acid (ester), polylactic acid, poly-
The one or several kinds in copolymer between hydroxybutyric acid, polyglycolic acid, PPDO and above-mentioned polymer, solvent can
For water, formic acid, acetic acid, chloroform, acetone, hexafluoroisopropanol, Hexafluoro acetone, tetrahydrofuran, N,N-dimethylformamide and above-mentioned
Two kinds or two or more mix in varing proportions.
5. inwall micro-patterning small-caliber artificial blood vessel according to claim 1, its electrostatic spinning microfibril layer passes through quiet
Prepared by Electrospinning, the solute of spinning solution used (can be selected one of following or following two for degradable high polymer material
Kind or two or more mixtures)：1. natural macromolecular material, including it is fibroin albumen, chitosan, collagen, elastin laminin, bright
2. glue protein synthesizes high polymer material, including polycaprolactone, polyurethane, polyglycolic acid (ester), polylactic acid, poly- hydroxyl fourth
Copolymer between acid, polyglycolic acid, PPDO and above-mentioned polymer, solvent are formic acid, acetic acid, chloroform, acetone, six
Fluorine isopropanol, Hexafluoro acetone, tetrahydrofuran, N,N-dimethylformamide and above two two or more are mixed in varing proportions
Close.
6. inwall micro-patterning small-caliber artificial blood vessel according to claim 1, it is characterised in that there is good mechanics
Performance, meet the requirement of transplanting in small-caliber artificial blood vessel body.
7. inwall micro-patterning small-caliber artificial blood vessel according to claim 1, it is characterised in that there is good biology
Compatibility, endothelial cell oriented growth can be guided, available for the engineered artificial blood vessel of external structure.
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| CN108619566A (en) * | 2018-05-24 | 2018-10-09 | 郑州大学 | Based on vascular basement membrane structure the multi-functional decorative layer of the bionical structure of cardiovascular implant material method |
| CN109908401A (en) * | 2019-03-11 | 2019-06-21 | 武汉杨森生物技术有限公司 | A kind of production method of artificial blood vessel and products thereof for promoting endothelial cell to seek connections with |
| CN114470321A (en) * | 2021-12-15 | 2022-05-13 | 深圳先进技术研究院 | Tubular nanofiber material and preparation method thereof |
| CN115305582A (en) * | 2022-07-15 | 2022-11-08 | 武汉理工大学 | Preparation method and application of neurovascularization double-sided bionic periosteum |
| CN115944785A (en) * | 2022-12-16 | 2023-04-11 | 上海工程技术大学 | Preparation method of homogeneous fiber tubular scaffold |
| WO2023108469A1 (en) * | 2021-12-15 | 2023-06-22 | 深圳先进技术研究院 | Tubular nanofiber material and preparation method therefor |
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| CN109908401A (en) * | 2019-03-11 | 2019-06-21 | 武汉杨森生物技术有限公司 | A kind of production method of artificial blood vessel and products thereof for promoting endothelial cell to seek connections with |
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| WO2023108469A1 (en) * | 2021-12-15 | 2023-06-22 | 深圳先进技术研究院 | Tubular nanofiber material and preparation method therefor |
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