CN102525689B - Orientated nano fiber bionic nerve conduit and manufacturing method thereof - Google Patents
Orientated nano fiber bionic nerve conduit and manufacturing method thereof Download PDFInfo
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Abstract
The invention provides an orientated nano fiber bionic nerve conduit and a manufacturing method thereof. A nano fiber membrane with orientation degree larger than or equal to 75% is prepared by means of coaxial electrostatic spinning, and then is curved to form the nerve conduit, NGF (nerve growth factor) is added into fibers so that the bionic nerve conduit is combined with various factors promoting nerve regeneration, such as 'nano bionics', 'contact guiding', 'drug release' and the like, and the bionic nerve conduit can effective promote nerve regeneration as proved by animal experiments.
Description
Technical field
The present invention relates to a kind of nerve rehabilitating tube and preparation method thereof, particularly relate to a kind of can nerve trachea promoting nerve growth and preparation method thereof fast.
Background technology
Nervous system controls sensorimotor's function of human body, neural once defect, then mean the forfeiture of some position sensation, motor function, bring great misery to patient, therefore CO2 laser weld and regeneration are the emphasis that people pay close attention to.Nerve autograft is the most classical method of clinical repair nerve injury, but to need to cut patient's readme healthy neural for the method, not only increases operative site, also may cause this innervation district dysfunction; Allograft then needs use in conjunction immunosuppressant, and transplanting succeed rate is lower.Therefore, the clinical reparation to peripheral nerve injury is not satisfactory for a long time, to thick, long section neurologic defect and multiple nerve injury at one's wit's end especially.
Since US Patent No. 4963146 discloses a kind of multilamellar, semi-permeable conduits, studied new nerve trachea and carried out the Bridging nerve broken ends of fractured bone, be a study hotspot to realize repairing peripheral nerve injury always.Nerve trachea normally makes suitable tubular bracket with biological or non-biological material, and Bridging nerve absolutely, while providing neuranagenesis microenvironment, promotes neuranagenesis by neural chemotactic induction, neurotrophic effect; Utilize nerve trachea to repair injured nerve to have the following advantages: alleviate the tension force sewing up mouth; Guide neuron axon axial growth, avoid external and form neuroma; For neuranagenesis provides the microenvironment of a relative insulation, prevent scar tissue from invading the nerve fiber of regeneration; Fix and support the two ends of defect nerve, improving the degree of accuracy that nerve tract is involutory; Simultaneously can in nerve trachea implantable neural trophic factors to improve neuranagenesis speed and quality.A kind of compound Polylactic Acid Extended Release artificial nerve catheter material and preparation method disclosed in patent CN1241656C, by polylactic acid, nanometer hydroxyapatite and NGF(nerve growth factor, nerve growth factor) mixing, make the composite conduit with micropore by solvent volatilization; Patent CN100539965C discloses a kind of absorptive nervous catheter and preparation method thereof, and chitosan and lactic acid are coated on mould rod, nerve trachea is made in the dry demoulding; Patent CN100479785C discloses a kind of preparation method of double-layer artificial nerve catheter, chitosan solution and collagen solution are poured respectively into obtained conduit in mould, then collagen protein conduit is inserted in chitosan catheter, and double-layer tube made by fiberfill fibers albumin glue between two-layer conduit.
But nerve trachea, except requiring to have except biocompatibility, is also wanted to keep steady statue in vivo, not easily subside and rupture, the concept that Lundborg proposes " inherent support ", forms inherent support in the tube chamber of fiber filaments being inserted nerve trachea, plays the effect of temperature matrix bridge.On this basis, patent CN101366980A, disclose a kind of double braid nerve trachea and preparation method thereof, polymeric polyglycolide-polylactide yarn is woven into inside and outside layer tube wall, then uses chitosan slurry outer, and inner layer tube wall is penetrated skin make braid nerve trachea; Patent CN101366981A then adopts the parallel layer bonding of polymeric polyglycolide-polylactide to make internal layer, forms braid nerve trachea together with the skin that polymeric polyglycolide-polylactide yarn is woven into; Patent CN101366982A then adopts non-woven fabrics of chitin to be internal layer, and polymeric polyglycolide-polylactide yarn is woven into skin and makes braid nerve trachea.Although above-mentioned patented technology can improve the intensity of nerve trachea, effect is also bad.
And nerve trachea disclosed in above-mentioned patented technology, what all adopt is the mode of horizontal orientation, and neuroregenerative effects is bad.
Summary of the invention
The invention provides a kind of nerve trachea be made up of oriented nanofibers, by the nano fibrous membrane making high-orientation that stretches, be then rolled into nerve trachea.Made nerve trachea has higher modulus and intensity in fiber orientation directions, and cell can be guided along fiber orientation directions (nerve trachea bearing of trend) growth, and neuranagenesis speed is accelerated, and compensate for the deficiency of traditional neural conduit performance.
Orientated nano fiber bionic nerve conduit of the present invention is the hollow pipe of the nanofiber composition that Biodegradable material is made, and its internal diameter is 0.5 ~ 5.0mm, and external diameter is 1.0 ~ 6.0mm; Described nanofiber diameter is 150nm ~ 1100nm, and institute's nanofiber is towards described nerve trachea bearing of trend orientation, the degree of orientation >=75%.
In the test of the degree of orientation of the present invention, with nerve trachea bearing of trend for benchmark, with this fiber of angle of inclination, direction within 15 °, be all defined as the fiber towards nerve trachea bearing of trend orientation.
Wherein, described Biodegradable material is preferably poly lactic coglycolic acid (PLGA).
Wherein, in described nerve trachea, the nanofiber degree of orientation is 50% ~ 100%.
According to a kind of preferred implementation of described orientated nano fiber bionic nerve conduit, wherein, described nanofiber is doughnut, is filled with the mixture comprising nerve growth factor (NGF) in cavity.
Preferably, described mixture also comprises Polyethylene Glycol (PEG).
Wherein, described NGF and PEG amount ratio is preferably 10 μ g:100 ~ 400mg.
Present invention also offers a kind of manufacture method of described bionic nano nerve trachea, wherein:
With biocompatible polymer solution for shell solution, Polyethylene Glycol is added as sandwich layer solution in the buffer solution of nerve growth factor, respectively by different injection vessel, two kinds of solution are converged at spinning nozzle, wherein shell solution is at skin, and sandwich layer solution is at internal layer;
Then Dispersion relation is made by the nano fibrous membrane having shell, the nanofiber of core double-layer structure forms, and wherein, stretches in spinning process, makes nanofiber uniaxial orientation;
Finally, with nanofiber differently-oriented directivity for axis, nano fibrous membrane is rolled into described bionical nerve trachea.
Wherein, be preferably and adopt rotating speed to be that the roller bearing of 4000 revs/min (4000rpm) stretches.
Wherein, be preferably electrostatic spinning technique and carry out Dispersion relation.
Wherein, described buffer solution to be pH value be 7.0 ~ 7.5 buffer solution, and more preferably phosphate buffered solution.
Orientated nano fiber bionic nerve conduit of the present invention, fibre orientation degree is high, nerve trachea tensile strength and shape maintains ability excellence.The machine-direction oriented cell that can guide aligns along fiber orientation directions (i.e. nerve trachea bearing of trend) simultaneously, thus better promotes neuranagenesis.
Nanofiber is made shell, cored structure, shell selects biodegradation material to make, and sandwich layer is nerve growth factor, makes that one can control, activated composite nerve conduit, can locally discharge activated nerve growth factor, the 26S Proteasome Structure and Function of simulation autologous nerve.
Add PEG in the core simultaneously, the filametntary uniformity can be improved, thus make the nerve trachea made have more excellent performance.
When adopting Dispersion relation technology, electric charge is only enriched in drop outer surface (shell), can provide natural, a gentle environment for core liquid (sandwich layer), the active substance being dispersed in sandwich layer from the impact of electric field force, thus can tolerate electrospinning process and non-loss of activity.
Accompanying drawing explanation
Fig. 1 is co-axial electrospinning process schematic.
Fig. 2 is embodiment of the present invention 1 bionical nerve trachea structure SEM photo;
Fig. 3 is the bionical nerve trachea fiber diameter distribution profile of embodiment of the present invention 1;
Fig. 4 is the bionical nerve trachea fiber shells of embodiment of the present invention 2, cored structure TEM photo;
Fig. 5 is embodiment of the present invention 2 bionical nerve trachea NGF elution profiles;
Fig. 6 is embodiment of the present invention 2 bionical nerve trachea zoopery electrophysiologic study nerve conduction velocity comparison diagram;
Fig. 7 is embodiment of the present invention 2 bionical nerve trachea zoopery electrophysiologic study action potential amplitude comparison diagram
Fig. 8 is that embodiment of the present invention 2 bionical nerve trachea zoopery wet weight of gastrocnemius muscle compares comparison diagram;
Fig. 9 is embodiment of the present invention 2 bionical nerve trachea zoopery histological examination comparison diagram;
Figure 10 is embodiment of the present invention 2 bionical nerve trachea zoopery regenerated nervous fibers number comparison diagram;
Figure 11 is embodiment of the present invention 2 bionical nerve trachea zoopery nervous tissue percentage ratio comparison diagram;
Figure 12 is embodiment of the present invention 2 bionical nerve trachea zoopery regenerating nerve Myelin thickness TEM photo;
Figure 13 is embodiment of the present invention 2 bionical nerve trachea zoopery regenerating nerve Myelin thickness comparison diagram;
Figure 14 is embodiment of the present invention 3 bionical nerve trachea nanofibers SEM photo.
Figure 15 is the bionical nerve trachea fiber diameter distribution profile of embodiment of the present invention 3.
Detailed description of the invention
Peripheral nerve injury is clinical common diseases, its poor prognosis, and disability rate is very high, brings huge economic loss and mental burden to patient, family and society.Therefore, how promoting neural regeneration, recovering the function of target organ is the focus paid close attention to always.Clinically for perineural from disconnected wound, normal adopt microsurgical technique to carry out holding-hold adventitia is sewed up, perineurial suture or combine stitching, to recover the seriality of nerve; For peripheral nerve defection, nerve autograft is still the main selection of repairing at present.But have that reproduction speed is slow, autologous nerve source of drawing material is limited, donor and the shortcoming such as receptor diameter nerve does not mate, also will cause the damage in confession district and certain dysfunction when cutting autologous nerve simultaneously.Therefore find the substitute of autologous nerve and promote that the method for neuranagenesis becomes the focus of research field.
In recent years, the rise of tissue engineering, for the treatment of numerous tissue defects, patient organ failure brings dawn.The neuranagenesis support utilizing biomaterial to build, not only can provide a favourable space for neurocyte acquisition nutrition, metabolism and growth, also there is the tension force alleviating and sew up mouth, guide the growth of nerve fiber, improve the degree of accuracy that nerve tract is involutory, prevent scar tissue from invading the advantages such as the nerve fiber of regeneration.Desirable neuranagenesis support should meet: the 1) structure of bionical natural extracellular matrix and biological function, 2) there is good biocompatibility that cell can be sticked and breed, 3) speed of degradation rate and tissue regeneration matches, 5) suitable mechanical performance, support Growth of Cells, 5) there is good microstructure, the hole dimension, high porosity and the pore morphology be connected that are such as applicable to, 6) surface physicochemical property that specific three-dimensional profile, 7) high-ratio surface sum is suitable
On this basis, orientated nano fiber bionic nerve conduit provided by the invention, be 150nm ~ 1100nm by diameter and the hollow pipe formed towards the nanofiber of described nerve trachea bearing of trend orientation, the degree of orientation is preferably 50% ~ 100%
Be described in detail oriented nanofibers nerve trachea of the present invention below by specific embodiment, to have made better understanding the present invention, but following embodiment does not limit the scope of the invention.
embodiment 1:
Orientated nano fiber bionic nerve conduit of the present invention, have the Biodegradable nanometer fiber of orientation to make, described nerve trachea internal diameter is 0.5 ~ 5.0mm, and external diameter is 1.0 ~ 6.0mm, and internal-and external diameter can adjust as required; The degree of orientation >=75%.
We are for PLGA, and adopt spining technology to make described nerve trachea, method is as follows:
Preparation PLGA solution, connects device for spinning, the spinning parameters such as adjustment solution flow rate, voltage, roller rotation rate, receiving range, the fibrous membrane of the nanofiber composition preparing uniaxial orientation that stretched by roller bearing; With nanofiber differently-oriented directivity for axis, make nerve fiber conduit by curling for fibrous membrane, the nerve trachea be rolled into can adopt the modes such as stitching or bonding to be fixedly connected with joint portion.
Under 10kv accelerating potential, scanning electron microscope (SEM) observes fibre morphology, and with reference to Fig. 2, visible orientated nano fiber bionic nerve conduit of the present invention all towards nerve trachea bearing of trend orientation, and has the higher degree of orientation, and the degree of orientation is 78.2% as calculated.
Application Image-J software is analyzed SEM photo, and random calculating 100 fibre diameters, evaluate average fibre diameter, diameter range and diameter Distribution, and with reference to Fig. 3, nanofiber diameter scope is 150nm ~ 550nm, and average diameter is 299 ± 67nm.
Due to the present invention's employing is that fiber is machine-direction oriented along nerve trachea, the cellulose of orientation can control cell orientation, and longitudinally strengthening cyton and interfibrous interaction, therefore oriented nanofibers nerve trachea of the present invention can guide cell to align along fibre orientation method (i.e. nerve trachea bearing of trend), thus better promotes neuranagenesis.
embodiment 2:
On the basis of embodiment 1, the nanofiber forming bionical nerve trachea of the present invention is doughnut, is filled with the bioactive substances such as nerve growth factor (NGF) in cavity, as albumen, medicine etc., thus composition shell, cored structure.
With reference to Fig. 1, for co-axial electrospinning technology, orientated nano fiber bionic nerve conduit manufacture method of the present invention is as follows:
Prepare the PLGA solution of 8% concentration as shell solution 6; Getting NGF lyophilized powder 10 μ g, to be dissolved into pH value be in the phosphate buffered solution (PBS) of 7.0 ~ 7.5, and stir the sandwich layer solution 5 obtained containing NGF; Get two syringes 1 and 2 and draw shell solution and sandwich layer solution respectively, connect coaxial electrostatic spinning silk device, shell solution is placed in skin, sandwich layer solution is placed in internal layer, the electrospinning parameters such as the rotating speed (for 4000 revs/min) of adjustment solution flow rate, voltage, receiving range, roller bearing 8, make the fibrous membrane that uniaxial orientation nanofiber 7 forms.Nerve trachea is made with reference to the mode described in embodiment 1.
Wherein, described electrostatic spinning technique refers to polymeric spray electrostatic stretch spin processes, and equipment therefor comprises HV generator 3, spinning appts receiving system 4 with tiny shower nozzle.
Under 175kv Accelerating electron rate, fibre structure observed by transmission electron microscope (TEM), and as shown in Figure 4, made fiber has obvious shell 12 and sandwich layer 11.
1) NGF slow release research
The nano fibrous membrane being loaded with NGF is put into the 24 hole culture dishs containing PBS, regularly get supernatant ELISA and detect NGF content.
Fig. 5 is the slow release NGF elution profiles feature of 1 month, and as can be seen from the figure, NGF release is divided into two stages, and within first 3 days, release phenomenon for prominent in early days, NGF release is about 29%; Afterwards then for evenly to discharge slowly.
2) animal experiment study
Rat 13mm Sciatic is repaired with the orientated nano fiber bionic nerve conduit bridge joint of made load NGF, 3 months after operation carries out EMG detection, sural nerve weight in wet base ratio, histological observation, electron-microscope scanning and the method such as graphical analysis and statistical analysis, the effectiveness of systematic analysis technique medicine carrying nerve trachea clinical practice and safety.And of the present invention group (PLGA/NGF) is contrasted with nerve autograft group (Autograft).
Electrophysiologic study nerve conduction velocity (with reference to Fig. 6) and action potential amplitude (with reference to Fig. 7) result display, when using the bionical nerve trachea of the present invention to repair, nerve recovery speed and nerve autograft group no difference of science of statistics.
Wet weight of gastrocnemius muscle ratio (with reference to Fig. 8) display, PLGA/NGF group gastrocnemius resume speed and nerve autograft group no difference of science of statistics.
Fig. 9 is that (A is PLGA/NGF group to regenerating nerve stage casing toluidines myelin staining contrast photo, B is nerve autograft group), Figure 10,11 is respectively regenerated nervous fibers number and nervous tissue's comparison diagram, can find from Fig. 9 ~ 11, and histological indications conforms to above-mentioned functions testing result.
TEM checks regenerating nerve Myelin thickness, result display PLGA/NGF group regenerating nerve comparatively ripe (Figure 12), Myelin thickness and nerve autograft group no difference of science of statistics (Figure 13).
embodiment 3:
On the basis of embodiment 2, the shell of the bionical nerve trachea of composition the present invention, the sandwich layer of cored structure fiber also comprise Polyethylene Glycol.
With reference to the method described in embodiment 2, orientated nano fiber bionic nerve conduit manufacture method of the present invention is as follows:
Prepare the PLGA solution of 8% concentration as shell solution; Getting NGF lyophilized powder 10 μ g, to be dissolved into pH value be in the phosphate buffered solution (PBS) of 7.2, is made into NGF solution, gets 400mgPEG and add in NGF solution that to be made into concentration be that the NGF/PEG solution of 400mg/ml is as sandwich layer solution.
Get syringe and draw sandwich layer solution and shell solution respectively, connect coaxial electrostatic spinning silk device, PLGA solution (shell solution) is placed in skin, NGF/PEG solution (sandwich layer solution) is placed in internal layer, adjustment electrospinning parameters, 4000 revs/min of rotating speeds stretch, and spinning obtains uniaxial orientation nano fibrous membrane.
Nerve trachea is made by curling for obtained nano fibrous membrane.
Carry out morphologic detection by SEM and Image-J software, can find out with reference to Figure 14, after adding Polyethylene Glycol, nanofibers obtains the higher uniformity.
With reference to Figure 15, obtained bionical nerve trachea diameter range is 200 ~ 1100nm, and average diameter is 513 ± 174nm.Compared with Fig. 3, after adding PEG, variation in fiber diameter is less, also illustrates that the fiber uniformity is improved.
embodiment 4:
On the basis of embodiment 3, in order to take into account the fiber uniformity, NGF content and be easy to spinning, PEG addition can adjust as required, as every 10 μ gNGF add 100mg, 200mg or 300mgPEG etc.
It should be noted that in the test of the degree of orientation of the present invention, with nerve trachea bearing of trend for benchmark, with this fiber of angle of inclination, direction within 15 °, be all defined as the fiber towards nerve trachea bearing of trend orientation.
In sum, the present invention utilizes coaxial electrostatic spinning to prepare the bionical nerve trachea of oriented nanofibers composition, this nerve trachea combines the factor of the multiple promotion neuranagenesis such as " nano bionic ", " contact guidance " and " medicament slow release ", proved by the scale-model investigation of rat 13mm Sciatic, this bionical nerve trachea effectively can promote neuranagenesis, and regenerating nerve function and form is close to nerve autograft group.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (6)
1. an orientated nano fiber bionic nerve conduit, it is characterized in that, described bionical nerve trachea is 150nm ~ 1100nm by diameter and the hollow pipe formed towards the nanofiber of described nerve trachea bearing of trend orientation, described bionical nerve trachea internal diameter is 0.5 ~ 5.0mm, and external diameter is 1.0 ~ 6.0mm; Described nanofiber is made up of Biodegradable material, the degree of orientation >=75%; Described Biodegradable material is poly lactic coglycolic acid; Described nanofiber is doughnut, is filled with the mixture comprising nerve growth factor in cavity; Also Polyethylene Glycol is comprised in described mixture; Nerve growth factor and Polyethylene Glycol amount ratio are 10 μ g:100 ~ 400mg.
2. a manufacture method for bionical nerve trachea as claimed in claim 1, is characterized in that,
With biocompatible polymer solution for shell solution, Polyethylene Glycol is added as sandwich layer solution in the buffer solution of nerve growth factor, respectively by different injection vessel, two kinds of solution are converged at spinning nozzle, wherein shell solution is at skin, and sandwich layer solution is at internal layer;
Then Dispersion relation is made by the nano fibrous membrane having shell, the nanofiber of core double-layer structure forms, and wherein, stretches in spinning process, makes nanofiber uniaxial orientation, the degree of orientation >=75%;
Finally, with nanofiber differently-oriented directivity for axis, nano fibrous membrane is rolled into described bionical nerve trachea.
3. manufacture method according to claim 2, is characterized in that, described in be stretched as and adopt rotating speed to be that the roller bearing of 4000 revs/min stretches.
4. manufacture method according to claim 2, is characterized in that, described buffer solution to be pH value be 7.0 ~ 7.5 buffer solution.
5. manufacture method according to claim 4, is characterized in that, described buffer solution is phosphate buffered solution.
6. manufacture method according to claim 2, is characterized in that, described Dispersion relation adopts electrostatic spinning technique.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL261533A (en) * | 2016-03-07 | 2018-10-31 | Univ Osaka | Sustained drug release sheet for treating nerve injury |
Families Citing this family (14)
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| CN104287869B (en) * | 2014-09-19 | 2017-03-29 | 上海市肺科医院 | A kind of novel nano tunica fibrosa/yarn count frame for graft of trachea and preparation method thereof |
| CN105455923A (en) * | 2015-11-24 | 2016-04-06 | 无锡中科光远生物材料有限公司 | Neural restoration sleeve tube and preparation method and application thereof |
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| GB202000714D0 (en) | 2020-01-17 | 2020-03-04 | Ucl Business | Biomaterials |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101100766A (en) * | 2007-07-13 | 2008-01-09 | 东华大学 | Preparation of nano fibre carrier for loading and slow releasing pharmaceutical and biologically active factor |
| CN101172164A (en) * | 2006-11-03 | 2008-05-07 | 中国科学院化学研究所 | Biopolymer nano tunica fibrosa material capable of being biological degraded and absorbed, preparing method and uses of the same |
| CN101363137A (en) * | 2008-09-24 | 2009-02-11 | 重庆大学 | Electrostatic spinning device for preparing directional arrangement nano fiber |
| CN101439205A (en) * | 2008-12-29 | 2009-05-27 | 上海市第六人民医院 | Nerve catheter as well as preparation method and application thereof |
| CN101509153A (en) * | 2009-03-23 | 2009-08-19 | 东华大学 | Method for producing shell-core structure medicament nano-fibre with coaxial electrostatic spinning technology |
| CN101912318A (en) * | 2010-07-30 | 2010-12-15 | 东华大学 | Three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof |
-
2010
- 2010-12-20 CN CN201010595866.3A patent/CN102525689B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101172164A (en) * | 2006-11-03 | 2008-05-07 | 中国科学院化学研究所 | Biopolymer nano tunica fibrosa material capable of being biological degraded and absorbed, preparing method and uses of the same |
| CN101100766A (en) * | 2007-07-13 | 2008-01-09 | 东华大学 | Preparation of nano fibre carrier for loading and slow releasing pharmaceutical and biologically active factor |
| CN101363137A (en) * | 2008-09-24 | 2009-02-11 | 重庆大学 | Electrostatic spinning device for preparing directional arrangement nano fiber |
| CN101439205A (en) * | 2008-12-29 | 2009-05-27 | 上海市第六人民医院 | Nerve catheter as well as preparation method and application thereof |
| CN101509153A (en) * | 2009-03-23 | 2009-08-19 | 东华大学 | Method for producing shell-core structure medicament nano-fibre with coaxial electrostatic spinning technology |
| CN101912318A (en) * | 2010-07-30 | 2010-12-15 | 东华大学 | Three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL261533A (en) * | 2016-03-07 | 2018-10-31 | Univ Osaka | Sustained drug release sheet for treating nerve injury |
| EP3427726A4 (en) * | 2016-03-07 | 2019-10-09 | Osaka University | Sustained drug release sheet for treating nerve damage |
| JP7057573B2 (en) | 2016-03-07 | 2022-04-20 | 国立大学法人大阪大学 | Sustained release sheet for nerve injury treatment |
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