CN107789517A - A kind of Chinese medicine compound prescription antivirus defervescent and preparation method thereof - Google Patents
A kind of Chinese medicine compound prescription antivirus defervescent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine compound prescription antivirus defervescent and preparation method thereof.In parts by weight, the Chinese medicine compound prescription antivirus defervescent is made up of following Chinese medicine material medicines：10 30 parts of folium isatidis, 120 200 parts of radix scutellariae, 120 200 parts of honeysuckle, 1 1.5 parts of calculus bovis factitius, 60 120 parts of the capsule of weeping forsythia, 20 40 parts of lophatherum gracile, 20 40 parts of great burdock achene, 0.2 2 parts of peppermint oil, 2 20 parts of extract of licorice root；And PH conditioning agents, and the weight of the PH conditioning agents meets：The pH value of the compound Chinese medicinal preparation is adjusted to 79.The Chinese medicine compound prescription antivirus defervescent of the present invention passes through the optimization to Extraction Process of Scuytellaria Baicalenksis and the use of PH conditioning agents, improve the absorption characteristic of Baical Skullcap root P.E, the bioavilability of Baical Skullcap root P.E is improved, so as to improve the antiviral drug activity of compound Chinese medicinal preparation.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, more particularly to a kind of Chinese medicine compound prescription antivirus defervescent and its preparation side
Method.
Background technology
Flu is a kind of common disease, is divided into common cold and influenza.Influenza is drawn by influenza virus
The Acute respiratory infectious disease risen.Cause the virus of influenza to include Respiratory Syncytial Virus(RSV), rhinovirus, adenovirus, hat
Shape virus and parainfluenza virus etc., usual influenza is than cold symptoms weight, and complication is more, and its incidence of disease is higher, it is wide to influence, often
The diseases such as nasosinusitis, sphagitis, bronchitis and pneumonia, asthma can be caused.
The method, doctor trained in Western medicine generally use anti-inflammation, antiallergy, antipyretic analgesic etc. of clinical treatment flu is suited the medicine to the illness square at present
Method or antiviral therapy.For example, anti-microbial type antiphlogistic is often with injection or medicinal preparation for oral administration such as penicillin, cephalos；Antiallergic is commonly used
The medicines such as chlorpheniramine；Antipyretic analgesic then commonly uses aspirin etc..And the traditional Chinese medical science generally according to flu type difference, using pungent-warm solution
Table and the treatment of the method for relieving the exterior syndrome with drugs pungent in flavor and cool in property.
A kind of antivirus defervescent is provided in patent of invention ZL03801243.X, its main Chinese medicine material includes big
Leafiness, radix scutellariae, honeysuckle, calculus bovis factitius, green grass or young crops stick up, lophatherum gracile, great burdock achene, peppermint etc., the antiviral antipyretic granule is for virus
The infection of the upper respiratory tract, pulmonary infection etc. are effective in cure caused by property influenza, common cold, but this kind of antiviral antipyretic granule still suffers from
Part to be modified.On the one hand, Baical Skullcap root P.E has been used in this kind of compound preparation, the main component of Baical Skullcap root P.E is radix scutellariae
Glycosides, it has many activity such as antibacterial, heat-clearing, anti-inflammatory, but due to the fat-soluble of scutelloside and is poorly soluble, and causes it
Absorption difference, bioavilability are low in vivo, drug effect is extremely unstable, so as to limit the clinical application effect of compound preparation.It is another
Aspect, it needs further to be improved in the drug effect of anti-virus aspect.
The content of the invention
It is an object of the invention to provide a kind of bioavilability is higher, the more preferable Chinese medicine compound prescription of antiviral activity is antiviral
Defervescence preparation.
Another object of the present invention is to provide the preparation method of above-mentioned Chinese medicine compound prescription antivirus defervescent.
The purpose of the present invention is achieved through the following technical solutions：
The present invention provides a kind of Chinese medicine compound prescription antivirus defervescent, in parts by weight, is made up of following Chinese medicine material medicines：
Folium isatidis 10-30 parts, radix scutellariae 120-200 parts, honeysuckle 120-200 parts, calculus bovis factitius 1-1.5 parts, capsule of weeping forsythia 60-120 parts, henon bamboo
Leaf 20-40 parts, great burdock achene 20-40 parts, peppermint oil 0.2-2 parts, extract of licorice root 2-20 parts；And PH conditioning agents, and the PH is adjusted
The weight for saving agent meets：The pH value of the compound Chinese medicinal preparation is adjusted to 7-9.
Further, the Chinese medicine material medicine of Chinese medicine compound prescription antivirus defervescent provided by the present invention also includes：Mountain beans
Root 10-20 parts.
Preferably, Chinese medicine compound prescription antivirus defervescent provided by the invention, in parts by weight, by following Chinese medicine material medicines
It is made：20 parts of folium isatidis, 160 parts of radix scutellariae, 160 parts of honeysuckle, 1.2 parts of calculus bovis factitius, 100 parts of the capsule of weeping forsythia, 30 parts of lophatherum gracile, burdock
30 parts of son, 1 part of peppermint oil, 15 parts of extract of licorice root, 15 parts of subprostrate sophora, and PH conditioning agents.
Wherein, the PH conditioning agents are sodium acid carbonate, sodium citrate or sodium carbonate.
Present invention simultaneously provides the preparation method of above-mentioned Chinese medicine compound prescription antivirus defervescent, following step is included：(1) with
Parts by weight meter weighs：Folium isatidis 10-30 parts, radix scutellariae 120-200 parts, honeysuckle 120-200 parts, calculus bovis factitius 1-1.5 parts, the capsule of weeping forsythia
60-120 parts, lophatherum gracile 20-40 parts, great burdock achene 20-40 parts, peppermint oil 0.2-2 parts, extract of licorice root 2-20 parts, and PH regulations
Appropriate agent；Above-mentioned each Chinese medicine material medicinal powder is broken；
(2) capsule of weeping forsythia, lophatherum gracile and great burdock achene are mixed, with water heating and refluxing extraction, extract solution filtering, obtains supernatant；
(3) by honeysuckle, 60~90% alcohol steep of folium isatidis, leaching liquor filtering, filtrate is obtained；
(4) by radix scutellariae water boiling and extraction, take filtrate to concentrate, with dense HCl regulation pH values to 1-2,10- is stood under 80 DEG C of constant temperature
14 hours, taking precipitate added water, and it is 4-7 to be washed with water to pH value, adds ethanol stirring and dissolving, and it is 7 to be washed till pH value, obtained radix scutellariae extraction
Thing；
(5) peppermint oil is added to 8-14 times of beta-schardinger dextrin, stirring inclusion 0.5-2 hours, filtered, 50-70 DEG C of dripping pill is done
It is dry, obtain peppermint oil inclusion compound；
(6) filtrate in the supernatant in step (2) and step (3) is mixed, is concentrated under reduced pressure, adds 65-70% second
Alcohol, 24-36 hours are stood, supernatant concentration is taken, adds calculus bovis factitius, peppermint oil inclusion compound, extract of licorice root and step (3)
In Baical Skullcap root P.E, PH conditioning agents are added after being well mixed, regulation pH value is made that Chinese medicine compound prescription is antiviral to bring down a fever to 7-9
Preparation.
Further, the preparation method of Chinese medicine compound prescription antivirus defervescent provided by the present invention also includes following steps
Suddenly：Subprostrate sophora 10-20 parts are weighed in parts by weight, are crushed, and after adding HCl standings, 80 DEG C of waters bath with thermostatic control extractions, filtering, are got it filled
Slag adds HC l, 60 DEG C of water bath with thermostatic control extractions, filtering, takes filtrate to be carried with 90-95% ethanol heating and refluxing extraction 2-3 hours
Take liquid to be concentrated after filtering, obtain sophorae subprostratae radix extract；By the sophorae subprostratae radix extract and the cow-bezoar, peppermint oil inclusion compound, sweet
Careless medicinal extract and Baical Skullcap root P.E add after the concentration in supernatant together.
Wherein, in above-mentioned steps (2), the temperature of heating and refluxing extraction is 70-80 DEG C, is extracted 1-4 times, each 30-60 points
Clock.In step (3), using alcohol steep 2-3 times.In above-mentioned subprostrate sophora extraction step, used HCl concentration is 10-
20%；80 DEG C of waters bath with thermostatic control are extracted 2-3 hours, and 1-2 hours are extracted in 60 DEG C of waters bath with thermostatic control.
It is further preferred that above-mentioned steps (4) also include：PEG1000, (NH are added into radix scutellariae glucoside extract4)2SO4With
Water, double-aqueous phase system is formed, stand split-phase, take phase, obtain aqueous two-phase extraction scutelloside, and aqueous two-phase extraction scutelloside is used
Chinese medicine compound prescription antivirus defervescent is prepared in step (6).
Wherein, PEG1000 weight/mass percentage composition is 32%, (NH in double-aqueous phase system4)2SO4Weight/mass percentage composition be
16%, the pH value of double-aqueous phase system is 7.8, and split-phase is obtained after standing 15 minutes at 30 DEG C.
It is further preferred that half biomimetic enzyme method extraction is carried out to sophorae subprostratae radix extract, the half biomimetic enzyme method extraction
Leaching agent is disodium hydrogen phosphate-citrate buffer solution, and extraction enzyme is cellulase, and extraction obtains subprostrate sophora liquid extract, by described in
Subprostrate sophora liquid extract is used to prepare the Chinese medicine compound prescription antivirus defervescent.
Wherein, carrying out the step of half biomimetic enzyme method extracts to sophorae subprostratae radix extract is：Phosphorus is added into sophorae subprostratae radix extract
Sour disodium hydrogen-citrate buffer solution, regulation pH value to 2.0, concussion is uniform, is filtered after standing 1.5 hours in 70 DEG C of water-baths；To
Disodium hydrogen phosphate-citrate buffer solution is added in filter residue, regulation pH value to 5.5, adds cellulase, concussion is uniform, at 70 DEG C
Filtered after standing 1.5 hours in water-bath；Disodium hydrogen phosphate-citrate buffer solution is added into filter residue, regulation pH value is 8.5, shake
Swing uniformly, filtered after standing 1.5 hours in 70 DEG C of water-baths；Merge No. 3 extract solutions, recycling design obtains subprostrate sophora liquid extract.
Compared with prior art, the present invention has advantages below：
(1) by the improvement to Extraction Process of Scuytellaria Baicalenksis and the use of PH conditioning agents, the absorption for improving Baical Skullcap root P.E is special
Property, so as to improve the antiviral activity of compound Chinese medicinal preparation：
The Main Ingredients and Appearance of Baical Skullcap root P.E is scutelloside, and it is a kind of flavone compound, has antibacterial, heat-clearing, anti-inflammatory
Etc. bioactivity.However, due to the fat-soluble of scutelloside and being poorly soluble, its absorption difference, biology profit in vivo is caused
Expenditure is low, drug effect is extremely unstable, so as to limit clinical practice.The plasma concentration curve of oral scutelloside is in multi-peaks phenomenon, and
Blood concentration is very low all the time, and its bioavilability is only 8% or so, and research shows biological utilisation of the compound compatibility to scutelloside
The improvement of degree also unobvious.Using scutelloside as in the oral compound preparation of component, the low blood concentration of scutelloside and low biology are sharp
Expenditure, often have a strong impact on its performance for cooperateing with drug action between other components in compound.
In general, in view of scutelloside belongs to flavone compound, and flavone compound contains phenolic hydroxyl group, is easy in acid
Property environment in the conventional cognitive that is stabilized, the final step of radix scutellariae extracting method is all to adjust pH value using acid in the prior art,
The Baical Skullcap root P.E for making to finally give is in sour environment.
The applicant have studied the Absolute oral profit after scutelloside the portal vein, filling Stomach duodenum and colon administration
Expenditure, it is found that scutelloside has no liver first-pass effect, liver first-pass effect is not the reason for scutelloside oral administration biaavailability is low.
Thus where speculating the barrier that intestines and stomach are scutelloside oral administration biaavailability, and absorption of the acid-base value for medicine in intestines and stomach
Using most important, so attempt by the change on Baical Skullcap root P.E pH value come influence its vivo biodistribution using process, improve
Absorption characteristic and bioavilability of the Baical Skullcap root P.E in intestines and stomach.On the other hand, radix scutellariae extracting method is optimized applicant,
The conventional cognitive of " scutelloside is easy to be stabilized in sour environment " is abandoned, last in radix scutellariae extraction step adds
The operation of " precipitation being washed till into pH value 7.0 with ethanol, dry ", the scutelloside for making to finally give are in neutral environment.It is in addition, logical
The use of PH conditioning agents is crossed, makes the pH value of compound preparation between 7-9.
Formed again with other components in the present invention by the Baical Skullcap root P.E obtained by after said extracted optimization order
Medicine compound preparation, after the compound preparation is oral, the bioavilability of Baical Skullcap root P.E has brought up to 26% by 8% or so, absorbs
Characteristic is improved, so that the antiviral drug effect of the compound preparation is also greatly improved.
(2) subprostrate sophora is added in the feed, to improve the clearing heat and detoxicating effect of the compound Chinese medicinal preparation；Improve mountain beans simultaneously
The extracting method of root, reduce the hepatotoxicity of sophorae subprostratae radix extract：
Subprostrate sophora is the dry root and rhizome of legume sophora tonkinensis Gapnep (also known as root of subprostrate sophora), is conventionally used to treatment fiery poison and pents up
Caused throat, gingivitis, for " solution abscess of throat first key medicine ".But the clinic poison of some document reports subprostrate sophora
Property, especially hepatotoxicity.
Subprostrate sophora mainly contains alkaloid, flavones, triterpene and polysaccharide isoreactivity composition, and main drug activity material is made a living
Alkaloids, current separated obtained alkaloid include matrine, oxymatrine, sparteine, N-Methylcytisine, Anagyris foetida
The kinds more than 20 such as alkali, sophoranol, Chinese scholartree peace, sophocarpine, N-Oxysophocarpine.The present invention is in the extraction step to subprostrate sophora, twice to mountain
Beans root herb using HCl extracted with water bath with thermostatic control after acid is heavy and then extracted with 90-95% alcohol reflux, is being retained
On the basis of the compositions such as main drug activity composition matrine, oxymatrine, other non-drug effects are made to live composition dissolutions, so as to
Reduce the toxicity of the compound preparation as caused by sophorae subprostratae radix extract.
(3) aqueous two-phase extraction is further carried out to radix scutellariae glucoside extract, in the premise that extraction time is short, reaction condition is gentle
Under, realize the further concentrating and purifying to scutelloside.
Scutelloside is Flavonoid substances, and the present invention is using Flavonoid substances in PEG1000 and (NH4)2SO4What is formed is double
The difference of distribution coefficient is extracted between aqueous phase.Present invention also offers the optimum response parameter of double-aqueous phase system, i.e.,：
PEG1000 weight/mass percentage composition is 32%, (NH4)2SO4Weight/mass percentage composition be 16%, pH value 7.8, in above-mentioned condition
Under, the yield of scutelloside reaches 89.6% to reaction system in rapid split-phase and upper phase in 15 minutes, obtains scutelloside composition
It is further to concentrate and purify.
(4) half biomimetic enzyme method extraction of progress further to sophorae subprostratae radix extract, alkaloid medicine in sophorae subprostratae radix extract is promoted
Imitate the dissolution of composition.
The half biomimetic enzyme method extraction that the present invention is carried out to sophorae subprostratae radix extract, simulation oral drugs are inhaled through gastrointestinal transit
The environment of receipts, promote the dissolution of effective ingredient.For the sophorae subprostratae radix extract in the present invention, delayed with disodium hydrogen phosphate-citric acid
Fliud flushing is leaching agent, and using cellulase as extraction enzyme, half biomimetic enzyme method extraction, institute are carried out using step provided by the present invention
Alkaloid in obtained subprostrate sophora liquid extract reaches 82.7%, has the characteristics of efficiency high, cycle is short, cost is low.
Embodiment
In this specification, unless otherwise specified, " part " refers to parts by weight；" % " is weight percentage.Unless otherwise specified, institute
It is this area normal experiment instrument and reagent with instrument, reagent.
Embodiment 1：Chinese medicine compound prescription antivirus defervescent 1
(1) weigh in parts by weight：20 parts of folium isatidis, 160 parts of radix scutellariae, 160 parts of honeysuckle, 1.2 parts of calculus bovis factitius, the capsule of weeping forsythia
100 parts, 30 parts of lophatherum gracile, 30 parts of great burdock achene, 1 part of peppermint oil, 15 parts of extract of licorice root, and PH conditioning agent sodium acid carbonates are appropriate；
Above-mentioned each Chinese medicine material medicinal powder is broken；
(2) capsule of weeping forsythia, lophatherum gracile and great burdock achene are mixed, with water heating and refluxing extraction, the temperature of heating and refluxing extraction is 80
DEG C, extract 4 times, 60 minutes every time, extract solution filtering, obtain supernatant；
(3) by honeysuckle, 90% alcohol steep 3 times of folium isatidis, leaching liquor filtering, filtrate is obtained；
(4) by radix scutellariae water boiling and extraction, take filtrate to concentrate, adjusted with dense HCl and stand 12 under pH value to 1.5,80 DEG C of constant temperature
Hour, taking precipitate adds water, and it is 5 to be washed with water to pH value, adds ethanol stirring and dissolving, and it is 7 to be washed till pH value, obtains Baical Skullcap root P.E；
(5) peppermint oil is added to 10 times of beta-schardinger dextrin, stirring inclusion 2 hours, filtering, 70 DEG C of dripping pills dryings, obtains peppermint
Oily inclusion compound；
(6) filtrate in the supernatant in step (2) and step (3) is mixed, is concentrated under reduced pressure, adds 70% ethanol,
30 hours are stood, supernatant concentration is taken, adds calculus bovis factitius, peppermint oil inclusion compound, extract of licorice root and Baical Skullcap root P.E, is mixed
PH conditioning agent sodium acid carbonates are added after closing uniformly, regulation pH value to 8, that is, Chinese medicine compound prescription antivirus defervescent are made.
Embodiment 2：Chinese medicine compound prescription antivirus defervescent 2
(1) weigh in parts by weight：20 parts of folium isatidis, 160 parts of radix scutellariae, 160 parts of honeysuckle, 1.2 parts of calculus bovis factitius, the capsule of weeping forsythia
100 parts, 30 parts of lophatherum gracile, 30 parts of great burdock achene, 1 part of peppermint oil, 15 parts of extract of licorice root, 15 parts of subprostrate sophora, and PH conditioning agent lemons
Lemon acid sodium is appropriate, and each Chinese medicine material medicinal powder is broken；
(2) capsule of weeping forsythia, lophatherum gracile and great burdock achene are mixed, with water heating and refluxing extraction, the temperature of heating and refluxing extraction is 80
DEG C, extract 4 times, 60 minutes every time, extract solution filtering, obtain supernatant；
(3) by honeysuckle, 70% alcohol steep 3 times of folium isatidis, take leaching liquor to filter, obtain filtrate；
(4) by radix scutellariae water boiling and extraction, filtrate is taken to concentrate, it is small to adjust standing 14 under pH value to 2,80 DEG C of constant temperature with dense HCl
When, taking precipitate adds water, and it is 6 to be washed with water to pH value, adds ethanol stirring and dissolving, and it is 7 to be washed till pH value, obtains Baical Skullcap root P.E；
(5) peppermint oil is added to 10 times of beta-schardinger dextrin, stirring inclusion 2 hours, filtering, 70 DEG C of dripping pills dryings, obtains peppermint
Oily inclusion compound；
(6) subprostrate sophora is taken, after adding HCl standings, 80 DEG C of waters bath with thermostatic control extractions, filtering, slag of getting it filled addition HCl, 60 DEG C of perseverances
Tepidarium is extracted, filtering, takes filtrate to be concentrated after extract solution filtering with 90% ethanol heating and refluxing extraction 3 hours, obtained mountain beans
Root extract；
(7) filtrate in the supernatant in step (2) and step (3) is mixed, is concentrated under reduced pressure, adds 65-70% second
Alcohol, 24-36 hours are stood, supernatant concentration is taken, adds calculus bovis factitius, peppermint oil, extract of licorice root, Baical Skullcap root P.E and mountain
Beans root extract, PH conditioning agents are added after being well mixed, regulation pH value to 7, that is, Chinese medicine compound prescription antivirus defervescent are made.
Embodiment 3：Chinese medicine compound prescription antivirus defervescent 3
(1) weigh in parts by weight：20 parts of folium isatidis, 160 parts of radix scutellariae, 160 parts of honeysuckle, 1.2 parts of calculus bovis factitius, the capsule of weeping forsythia
100 parts, 30 parts of lophatherum gracile, 30 parts of great burdock achene, 1 part of peppermint oil, 15 parts of extract of licorice root, 15 parts of subprostrate sophora, and PH conditioning agent lemons
Lemon acid sodium is appropriate, and each Chinese medicine material medicinal powder is broken；
(2) capsule of weeping forsythia, lophatherum gracile and great burdock achene are mixed, with water heating and refluxing extraction, the temperature of heating and refluxing extraction is 80
DEG C, extract 4 times, 60 minutes every time, extract solution filtering, obtain supernatant；
(3) by honeysuckle, 70% alcohol steep 3 times of folium isatidis, take leaching liquor to filter, obtain filtrate；
(4) by radix scutellariae water boiling and extraction, filtrate is taken to concentrate, it is small to adjust standing 14 under pH value to 2,80 DEG C of constant temperature with dense HCl
When, taking precipitate adds water, and it is 6 to be washed with water to pH value, adds ethanol stirring and dissolving, and it is 7 to be washed till pH value, obtains Baical Skullcap root P.E；To
PEG1000, (NH are added in radix scutellariae glucoside extract4)2SO4And water, double-aqueous phase system is formed, PEG1000 in the double-aqueous phase system
Weight/mass percentage composition for 32%, (NH4)2SO4Weight/mass percentage composition be 16%, the pH value of the double-aqueous phase system is 7.8,
Split-phase is obtained after standing 15 minutes at 30 DEG C, phase is taken, obtains aqueous two-phase extraction scutelloside；
(5) peppermint oil is added to 10 times of beta-schardinger dextrin, stirring inclusion 2 hours, filtering, 70 DEG C of dripping pills dryings, obtains peppermint
Oily inclusion compound；
(6) subprostrate sophora is taken, after adding HCl standings, 80 DEG C of waters bath with thermostatic control extractions, filtering, slag of getting it filled addition HCl, 60 DEG C of perseverances
Tepidarium is extracted, filtering, takes filtrate to be concentrated after extract solution filtering with 90% ethanol heating and refluxing extraction 3 hours, obtained mountain beans
Root extract；Disodium hydrogen phosphate-citrate buffer solution is added into the sophorae subprostratae radix extract, regulation pH value to 2.0, concussion is
It is even, filtered after standing 1.5 hours in 70 DEG C of water-baths；Disodium hydrogen phosphate-citrate buffer solution is added into filter residue, adjusts pH value
To 5.5, cellulase is added, concussion is uniform, is filtered after standing 1.5 hours in 70 DEG C of water-baths；Phosphoric acid hydrogen is added into filter residue
Disodium-citrate buffer solution, regulation pH value are 8.5, and concussion is uniform, is filtered after standing 1.5 hours in 70 DEG C of water-baths；Merge 3
Secondary extract solution, recycling design obtain subprostrate sophora liquid extract；
(7) filtrate in the supernatant in step (2) and step (3) is mixed, is concentrated under reduced pressure, adds 65-70% second
Alcohol, 24-36 hours are stood, supernatant concentration is taken, adds calculus bovis factitius, peppermint oil, extract of licorice root, aqueous two-phase extraction radix scutellariae and carry
Thing and subprostrate sophora liquid extract are taken, PH conditioning agents are added after being well mixed, regulation pH value to 7, that is, it is antiviral that Chinese medicine compound prescription are made
Defervescence preparation.
Embodiment 4：Contrast antivirus defervescent
Control antivirus defervescent is prepared according to the method provided in patent of invention ZL03801243.X：
Chinese medicine material medicine includes：10 parts of folium isatidis, 120 parts of radix scutellariae, 120 parts of honeysuckle, 1 part of calculus bovis factitius, green grass or young crops stick up 60 parts,
20 parts of lophatherum gracile, 20 parts of ox, 20 parts of peppermint simultaneously add 4 parts of flavouring extract of licorice root, PH conditioning agent sodium acid carbonates.
Its preparation method is as follows：
(1) folium isatidis is extracted three times with 70% 8 times of amounts of ethanol, each 6-8 hours, merges leaching liquor, then filter,
Obtain filtrate.
(2) peppermint is subjected to distillating extracting oil, adds beta-schardinger dextrin to wrap up.
(3) radix scutellariae is extracted into acid precipitation method with boiling, extracts scutelloside.
(4) by honeysuckle, green grass or young crops sticks up, Niu Zi, lophatherum gracile mix, add 10 times of amount water, extract 2 times；8 times of amount water extractions the 3rd
It is secondary；
(5) extract solution is merged and is concentrated under reduced pressure into 1:1 concentration, 65% ethanol is added, staticly settled 48 hours；
(6) leaching supernatant, it is concentrated into and adds extract of licorice root；
(7) peppermint oil wrappage, calculus bovis factitius, scutelloside and PH conditioning agent sodium acid carbonates are added, regulation pH value is 6.5,
Obtain contrast antivirus defervescent.
Embodiment 5：In vitro antibacterial test
1. materials and methods：
1.1 cell model:Vero cells；Mdck cell
It is 1.2 viral:Herpes simplex virus (HSV-I)；Respiratory Syncytial Virus(RSV) (RSV)；H3N2 type influenza viruses
1.3. the inhibitory action that CPE (cytopathic effect) determines samples for viral is observed
1.4 laboratory samples are prepared：
Laboratory sample is first configured to 20mg/ml with dimethyl sulfoxide DMSO.Then difference is diluted to respectively with cell culture fluid
Concentration.
1.5 detection process:
1. external anti-herpesvirus and Respiratory Syncytial Virus(RSV) experiment
Vero cells are inoculated in 96 well culture plates, after culture forms cell monolayer, add herpesviral or respiratory syncystial
Virus liquid infection cell 2 hours, then change to cell maximal non-toxic concentration (concentration during cytoactive >=90%) below
Sample diluting liquid, then after culture in 72 hours, micro- Microscopic observation cytopathy (CPE), determine suppression of the sample to herpesviral
Make and use.
2. external resisiting influenza virus experiment
Mdck cell is inoculated in 96 well culture plates, and after culture forms cell monolayer, it is small to add influenza virus liquid infection cell 2
When, the sample diluting liquid below to cell maximal non-toxic concentration (concentration during cytoactive >=90%) is then changed to, then through 72
After hour culture, micro- Microscopic observation cytopathy (CPE), the inhibitory action of sample infected by influenza is determined.
2. testing result:
2.1 external anti-herpes simplex virus HSV-I effects
2.2 external anti respiratory syncytial virus effects
The external resisiting influenza virus effect of 2.3 samples
3. conclusion:
The Chinese medicine compound prescription antivirus defervescent of the present invention is to herpes simplex virus (HSV-I), Respiratory Syncytial Virus(RSV)
(RSV), the inhibitory action of H3N2 types influenza virus is superior to the antivirus defervescent prepared in embodiment 3.
Claims (10)
1. a kind of Chinese medicine compound prescription antivirus defervescent, it is characterised in that in parts by weight, be made up of following Chinese medicine material medicines：
Folium isatidis 10-30 parts, radix scutellariae 120-200 parts, honeysuckle 120-200 parts, calculus bovis factitius 1-1.5 parts, capsule of weeping forsythia 60-120 parts,
Lophatherum gracile 20-40 parts, great burdock achene 20-40 parts, peppermint oil 0.2-2 parts, extract of licorice root 2-20 parts；
And PH conditioning agents, and the weight of the PH conditioning agents meets：The pH value of the compound Chinese medicinal preparation is adjusted to 7-9.
2. Chinese medicine compound prescription antivirus defervescent according to claim 1, it is characterised in that the Chinese medicine material medicine also wraps
Contain：Subprostrate sophora 10-20 parts.
3. Chinese medicine compound prescription antivirus defervescent according to claim 2, it is characterised in that in parts by weight, by following
Chinese medicine material medicine is made：
20 parts of folium isatidis, 160 parts of radix scutellariae, 160 parts of honeysuckle, 1.2 parts of calculus bovis factitius, 100 parts of the capsule of weeping forsythia, 30 parts of lophatherum gracile, burdock
30 parts of son, 1 part of peppermint oil, 15 parts of extract of licorice root, 15 parts of subprostrate sophora, and PH conditioning agents.
4. Chinese medicine compound prescription antivirus defervescent according to any one of claim 1 to 3, it is characterised in that the PH
Conditioning agent is sodium acid carbonate, sodium citrate or sodium carbonate.
5. the preparation method of the Chinese medicine compound prescription antivirus defervescent described in claim 1, it is characterised in that include following steps
Suddenly：
(1) weigh in parts by weight：Folium isatidis 10-30 parts, radix scutellariae 120-200 parts, honeysuckle 120-200 parts, calculus bovis factitius 1-
1.5 parts, capsule of weeping forsythia 60-120 parts, lophatherum gracile 20-40 parts, great burdock achene 20-40 parts, peppermint oil 0.2-2 parts, extract of licorice root 2-20 parts,
And PH conditioning agents are appropriate；Above-mentioned each Chinese medicine material medicinal powder is broken；
(2) capsule of weeping forsythia, lophatherum gracile and great burdock achene are mixed, with water heating and refluxing extraction, extract solution filtering, obtains supernatant；
(3) by honeysuckle, folium isatidis 60-90% alcohol steep, leaching liquor filtering, filtrate is obtained；
(4) by radix scutellariae water boiling and extraction, filtrate is taken to concentrate, it is small to stand 10-14 with dense HCl regulation pH values to 1-2, under 80 DEG C of constant temperature
When, taking precipitate adds water, and it is 4-7 to be washed with water to pH value, adds ethanol stirring and dissolving, and it is 7 to be washed till pH value, obtains Baical Skullcap root P.E；
(5) peppermint oil is added to 8-14 times of beta-schardinger dextrin, stirring inclusion 0.5-2 hours, filtering, 50-70 DEG C of dripping pill drying, obtained
Peppermint oil inclusion compound；
(6) filtrate in the supernatant in step (2) and step (3) is mixed, is concentrated under reduced pressure, adds 65-70% ethanol, it is quiet
24-36 hours are put, take supernatant concentration, add the calculus bovis factitius, peppermint oil inclusion compound, extract of licorice root and radix scutellariae extraction
Thing, PH conditioning agents are added after being well mixed, regulation pH value to 7-9, that is, Chinese medicine compound prescription antivirus defervescent are made.
6. preparation method according to claim 5, it is characterised in that also comprising following step：In parts by weight, mountain is weighed
Beans root 10-20 parts, crush, after adding HCl standings, 80 DEG C of waters bath with thermostatic control extractions, filter, slag of getting it filled adds HCl, 60 DEG C of thermostatted waters
Bath extraction, filtering, filtrate is taken to be concentrated after extract solution filtering with 90-95% ethanol heating and refluxing extraction 2-3 hours, obtain mountain
Beans root extract；
The sophorae subprostratae radix extract is added together with the cow-bezoar, peppermint oil inclusion compound, extract of licorice root and Baical Skullcap root P.E
In supernatant after the concentration.
7. preparation method according to claim 5, it is characterised in that the step (4) also includes：Carried to the scutelloside
Take and PEG1000, (NH are added in thing4)2SO4And water, double-aqueous phase system is formed, split-phase is stood, takes phase, obtain aqueous two-phase extraction
Scutelloside, and by the aqueous two-phase extraction scutelloside for preparing the Chinese medicine compound prescription antivirus defervescent in step (6).
8. preparation method according to claim 7, it is characterised in that PEG1000 quality hundred in the double-aqueous phase system
It is 32%, (NH to divide content4)2SO4Weight/mass percentage composition be 16%, the pH value of the double-aqueous phase system is 7.8, at 30 DEG C
Split-phase is obtained after standing 15 minutes.
9. preparation method according to claim 6, it is characterised in that half biomimetic enzyme method is carried out to the sophorae subprostratae radix extract
Extraction, the leaching agent of the half biomimetic enzyme method extraction is disodium hydrogen phosphate-citrate buffer solution, and extraction enzyme is cellulase, is carried
Subprostrate sophora liquid extract is obtained, the subprostrate sophora liquid extract is used to prepare the Chinese medicine compound prescription antivirus defervescent.
10. preparation method according to claim 6, it is characterised in that half bionic enzyme is carried out to the sophorae subprostratae radix extract
Method extract the step of be：Addition disodium hydrogen phosphate-citrate buffer solution into the sophorae subprostratae radix extract, regulation pH value to 2.0,
Concussion is uniform, is filtered after standing 1.5 hours in 70 DEG C of water-baths；Disodium hydrogen phosphate-citrate buffer solution is added into filter residue, is adjusted
PH value is saved to 5.5, adds cellulase, concussion is uniform, is filtered after standing 1.5 hours in 70 DEG C of water-baths；Added into filter residue
Disodium hydrogen phosphate-citrate buffer solution, regulation pH value are 8.5, and concussion is uniform, is filtered after standing 1.5 hours in 70 DEG C of water-baths；
Merge No. 3 extract solutions, recycling design obtains subprostrate sophora liquid extract.
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