CN107126465A - A kind of Chinese medicine composition with heat-clearing toxin-expelling functions and preparation method thereof and pharmaceutical preparation - Google Patents
A kind of Chinese medicine composition with heat-clearing toxin-expelling functions and preparation method thereof and pharmaceutical preparation Download PDFInfo
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Abstract
The present invention relates to a kind of Chinese medicine composition with heat-clearing toxin-expelling functions and preparation method thereof and pharmaceutical preparation, belong to the field of Chinese medicines.A kind of Chinese medicine composition with heat-clearing toxin-expelling functions, the Chinese medicine composition contains scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-accuse according to spring and total volatile oil, scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement are (150-600) according to the weight ratio of spring and total volatile oil：(10-60)：(1-6)：(10-50)：(2-9)：(50-200), wherein, total volatile oil is extracted by honeysuckle, the capsule of weeping forsythia and Radix Isatidis and formed.The Chinese medicine composition active constituent content of the present invention is high, product good effect.
Description
Technical field
The present invention relates to the field of Chinese medicines, in particular to a kind of Chinese medicine composition with heat-clearing toxin-expelling functions and its
Preparation method and pharmaceutical preparation.
Background technology
Traditional medicine thinks that " warm disease is from the beginning of then heating, micro- aversion to wind and cold, lossless or have sweat not smooth；Violate in wind-heat so that pharynx
Bitterly, cough；Warm heresy easily hinders body fluid, therefore thirsty；Zhi Dangxin cools completely table, clearing heat and detoxicating.", honeysuckle, the capsule of weeping forsythia, Radix Isatidis are pungent
Cool completely table, clearing heat and detoxicating good medicine.Radix scutellariae is labiate, is used as medicine with root, bitter, cold in nature, energy heat-clearing and damp-drying drug, purging intense heat solution
Poison, hemostasis, it is antiabortive have heat-clearing and damp-drying drug, cool blood is antiabortive, detoxicating functions.
Though honeysuckle, radix scutellariae, the capsule of weeping forsythia and Radix Isatidis are China's traditional Chinese medicine, in order that the drug regimen after compatibility
Thing can preferably play curative effect, for a long time, and those skilled in the art are always in its extraction process of constantly improve.It is universal at present
The extraction process of use is the four traditional Chinese medicines such as honeysuckle are carried out to decocting jointly to boil to be prepared, but Product Process also has with curative effect
Treat further raising.
The content of the invention
An object of the present invention is that there is provided a kind of Chinese medicine composition with heat-clearing toxin-expelling functions, its active ingredient
Content is high, product good effect.
The second object of the present invention is there is provided a kind of preparation method of the Chinese medicine composition with heat-clearing toxin-expelling functions,
Its technique is simple, improve active principle content in product, improves product curative effect.
The third object of the present invention is that there is provided a kind of its clearing heat and detoxicating effect of pharmaceutical preparation with heat-clearing toxin-expelling functions
Significantly, to virus infection, inflammation good effect.
What embodiments of the invention were realized in：
A kind of Chinese medicine composition with heat-clearing toxin-expelling functions, the Chinese medicine composition contains scutelloside, chlorogenic acid, reseda
Glycosides, Forsythoside a, (R, S)-accuse according to spring and total volatile oil, scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-
It is (150-600) to accuse according to the weight ratio of spring and total volatile oil：(10-60)：(1-6)：(10-50)：(2-9)：(50-
200), wherein, total volatile oil by honeysuckle, the capsule of weeping forsythia and Radix Isatidis extract form.
In preferred embodiments of the present invention, above-mentioned Chinese medicine composition also includes forsythin；Preferably, Chinese medicine composition contains
Some scutellosides, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement are according to the weight ratio of spring, total volatile oil and forsythin
(180-560)：(12-52)：(1-7)：(11-35)：(2-6)：(52-182)：(6-26).
A kind of preparation method of the Chinese medicine composition with heat-clearing toxin-expelling functions, including：
400-600 parts of 400-600 parts of the honeysuckle counted by weight, 800-1200 parts of the capsule of weeping forsythia and Radix Isatidis are existed
The 3-12 times of water distillation measured is extracted, and extraction is obtained into distillate and distilled again, obtains rectifying liquid, and rectifying liquid is total volatile oil；
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia and Radix Isatidis are subjected to decocting extraction, first is obtained
Aqueous extracts；The aqueous solution obtained when being extracted with the first Aqueous extracts with distillation prepares the first extract；
300-600 parts of the radix scutellariae counted by weight is subjected to decocting extraction in pH value is 7-8 water, second is obtained
Aqueous extracts, the second extract is prepared with the second Aqueous extracts；And mix total volatile oil, the first extract and the second extract,
Obtain containing scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement according to spring and the Chinese medicine composition of total volatile oil,
And scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement are (150- according to the weight ratio of spring and total volatile oil
600)：(10-60)：(1-6)：(10-50)：(2-9)：(50-200).
In preferred embodiments of the present invention, above-mentioned preparation method also includes：Total volatile oil is included with cyclodextrin,
Then dry.
According to weight ratio it is 1 by total volatile oil and cyclodextrin in preferred embodiments of the present invention：The ratio of (5-20)
Temperature be 30-60 DEG C, mixing time be to be included under conditions of 1-8h.
In preferred embodiments of the present invention, preparing the first extract includes：Obtained when first Aqueous extracts and distillation are extracted
To the aqueous solution filter, concentrate respectively, be then combined with, stir, add after ethanol solution, standing and filter in whipping process, receive
Collection supernatant simultaneously reclaims ethanol to solution without ethanol flavor, obtains the first extract.
In preferred embodiments of the present invention, preparing the second extract includes：PH value is adjusted after second Aqueous extracts are filtered
To 1-2, insulation is placed, filtering, collects precipitation, plus ethanol solution cleaning, and then suction filtration, drying obtains the second extract.
A kind of pharmaceutical preparation with heat-clearing toxin-expelling functions, by above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or
Diluent is constituted.
In preferred embodiments of the present invention, said medicine preparation is mixture, syrup, tablet, granule or capsule.
In preferred embodiments of the present invention, when pharmaceutical preparation be mixture when, mixture specification be 6 mls/branch, 10 milliliters/
Branch, 12 mls/branch or 20 mls/branch, preparing the method for mixture includes：Chinese medicine composition is dissolved in water, addition accounts for medicinal material
Total amount 2-10% sucrose or 0.2-1% Steviosin, mixing makes dissolving, adds water to ormal weight, boils 10-20min,
PH value is adjusted when temperature is down to below 80 DEG C to 6-7, then filtration, filling, sterilizing, are produced.1-7
The beneficial effect of the embodiment of the present invention is：
The Chinese medicine composition with heat-clearing toxin-expelling functions of the embodiment of the present invention include scutelloside, chlorogenic acid, galuteolin,
The total volatile oil that a parts of Forsythoside and (R, S)-announcement are extracted according to the spring and by honeysuckle, the capsule of weeping forsythia and Radix Isatidis.Honeysuckle
Volatile oil, Forsythia volatile oil and the pharmacological action of Radix Isatidis volatile oil significantly, while with scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a and (R, S)-announcement play synergy according to spring compatibility so that the Chinese medicine composition is clearing heat and detoxicating and sick in treatment
Poison infection and aspect of inflammation have more preferably therapeutic effect.The Chinese medicine composition with heat-clearing toxin-expelling functions of the embodiment of the present invention
Preparation method honeysuckle, the capsule of weeping forsythia are waved, Radix Isatidis carries out the extraction of volatile oil jointly, then by the gold and silver flower pesticide after extraction
Slag, the capsule of weeping forsythia are waved the dregs of a decoction, the Radix Isatidis dregs of a decoction and radix scutellariae and separately decocted, continue to extract scutelloside, chlorogenic acid, Forsythoside a and (R,
S)-accuse according to active ingredients such as spring so that the active ingredient in medicinal material is fully extracted, it is to avoid the loss of active ingredient, product
Quality is ensured with curative effect.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted production firm person of instrument, be
The conventional products that can be obtained by commercially available purchase.
After honeysuckle, the capsule of weeping forsythia, Radix Isatidis and radix scutellariae compatibility, the existing malicious antibacterial of suppression excites and strengthens body cell again
The physiological effect such as immunologic function and enhancing phagocytic function, with more antivirus action and obvious antibacterial action, especially to gold
The inhibitory action of Portugal bacterium, MRSE and proteus is stronger.And can strengthen the defense function of body, improve microcirculation and
Integrally-regulated effect.The virus that is particularly suitable for use in infection, virus and bacteria mixed infection disease, produce the disease of antibody-resistant bacterium, should
The not notable protracted inflammation unhealed of curative effect with antibiotic allergy sufferers and use antibiotic.Meanwhile, also to acute upper respiratory tract sense
The disease such as dye, acute tracheitis, bronchitis, acute tonsillitis short term effect is significantly, also effective to Bronchopneumonia.
The applicant has found Forsythia volatile oil, volatile honeysuckle oil, Radix Isatidis volatile oil pharmacology by a large amount of zooperies
Effect is notable.The clearing heat and detoxicating effect of volatile honeysuckle oil is notable；Forsythia volatile oil has clear and definite antiinflammatory action；Radix Isatidis volatilizees
Oily antiinflammatory action is obvious.
Also, applicant have also found that, the medicine of honeysuckle, the capsule of weeping forsythia, Radix Isatidis and radix scutellariae is utilized in the prior art
During formula, volatile honeysuckle oil, Forsythia volatile oil, the pharmacological action of Radix Isatidis volatile oil are have ignored just, simply merely will
Medicinal material is by decocting come effective component extracting.During extraction, due to the partial volume of volatile oil ten as one of active ingredient
Volatile, therefore, the content that oil is played in the water extract finally given is seldom, and most of volatile oil are waved during extraction
Diverging is lost, and causes loss of effective components, so as to have impact on the curative effect of product.
Scutelloside has significant bioactivity, with the pharmacological action such as purging intense heat and detonicating, antibacterial, anti-inflammatory, diuresis.Scutelloside
Water is slightly soluble in, ethanol, acetone is insoluble in, is slightly soluble in chloroform and nitrobenzene, be soluble in alkaline solution.It is dissolved in strong alkali solution
First displaing yellow, is then changed into dark brown soon.Scutelloside determines radix scutellariae traditional with other medicinal material water due to the property of itself
Carry in alcohol precipitation process, scutelloside fails to be efficiently transferred in product, have lost most of scutelloside.
Therefore, existing product technique needs to be further improved raising.
In addition, applicant have also found that, only there is lower bound requirement to scutelloside, chlorogenic acid content in the prior art, not to tool
The content and scope for having the compositions such as the Forsythoside a of very strong anti-infectious function and refrigeration function are defined, and this is also limitation
The reason for product quality is difficult to further effectively lifting.
After the crux of problem is cleared, the first aspect of the invention is there is provided a kind of with heat-clearing toxin-expelling functions
Chinese medicine composition, the Chinese medicine composition contains scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement according to the spring and always
Volatile oil, scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement are according to the weight ratio of spring and total volatile oil
(150-600)：(10-60)：(1-6)：(10-50)：(2-9)：(50-200), wherein, total volatile oil is by honeysuckle, company
Stick up to extract with Radix Isatidis and form.The Chinese medicine composition with heat-clearing toxin-expelling functions of the embodiment of the present invention contains pharmacological action and shown
The main total volatile oil based on volatile honeysuckle oil, Forsythia volatile oil and Radix Isatidis volatile oil write, can give play to more preferably
Clearing heat and detoxicating and anti-inflammatory efficacy, while compatibility scutelloside, chlorogenic acid, galuteolin, forsythin and (R, S)-accuse have according to spring etc.
Composition is imitated, Forsythoside a is especially contained, curative effect of the product in terms of removing toxic substances, anti-inflammatory is further improved.
Further, the Chinese medicine composition of the embodiment of the present invention also includes forsythin.Preferably, Chinese medicine composition contains
Scutelloside, chlorogenic acid, galuteolin, Forsythoside a, (R, S)-announcement are according to the weight ratio of spring, total volatile oil and forsythin
(180-560)：(12-52)：(1-7)：(11-35)：(2-6)：(52-182)：(6-26).
The second aspect of the invention there is provided a kind of preparation method of the Chinese medicine composition with heat-clearing toxin-expelling functions,
Including following process：
First, the preparation of total volatile oil
400-600 parts of 400-600 parts of the honeysuckle counted by weight, 800-1200 parts of the capsule of weeping forsythia and Radix Isatidis are existed
The 3-12 times of water distillation measured is extracted, and extraction is obtained into distillate and distilled again, rectifying liquid is obtained.Rectifying liquid is to include gold and silver
Flowers volatile oil, Forsythia volatile oil and Radix Isatidis play the total volatile oil of oil.Collect the aqueous solution obtained after distillation and the dregs of a decoction are standby.
By distilling one of active ingredient in honeysuckle, the capsule of weeping forsythia and Radix Isatidis：Volatile oil, is individually extracted, and is kept away
Non-volatile oil loses in the process volatilization of decoction, so as to improve the content of active ingredient in product.
Preferably, distill for steam distillation.
It is highly preferred that when carrying out distillation extraction to above-mentioned honeysuckle, the capsule of weeping forsythia and Radix Isatidis, extraction time is 1-3 times,
1-5h is extracted every time.The distillate that each distillation is obtained merges, and distills again, obtains rectifying liquid.
Further, above-mentioned total volatile oil and cyclodextrin are included, then dried.Preferably, by above-mentioned total volatilization
Oil is 1 according to weight ratio with cyclodextrin：The ratio of (5-20) is 30-60 DEG C, under conditions of mixing time is 1-8h in temperature
Included.
2nd, the preparation of the first extract
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, the first water is obtained
Extract.Extracted by decocting and extract the nonvolatile matter of honeysuckle, the capsule of weeping forsythia, Radix Isatidis, such as chlorogenic acid, Forsythoside
A and (R, S)-announcement are according to the spring.
Then, the aqueous solution obtained when being extracted with the first Aqueous extracts with distillation prepares the first extract.First is prepared to extract
Thing includes：The aqueous solution obtained when first Aqueous extracts and distillation are extracted is filtered, concentrated respectively.Then, merge, stir, stirring
Add after ethanol solution, standing and filter during mixing.Collect supernatant and reclaim ethanol to solution without ethanol flavor, obtain first and carry
Take thing.First extract can be understood as the mixed extract of honeysuckle, the capsule of weeping forsythia and Radix Isatidis.Preferably, ethanol solution is dense
Spend for 60-85% (volume ratio).
Preferably, when the dregs of a decoction of the dregs of a decoction of above-mentioned honeysuckle, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are carried out into decocting extraction, water
It is 1-3 times to decoct number of times, and 1-2h is decocted every time.The Aqueous extracts that each decocting is obtained merge, then by after Aqueous extracts and filtering
The above-mentioned aqueous solution merge, refilter concentration.
3rd, the preparation of the second extract
300-600 parts of the radix scutellariae counted by weight is subjected to decocting extraction in pH value is 7-8 water, second is obtained
Aqueous extracts.When carrying out decocting extraction, it is preferable that extract 2-3 times, each decocting time is 1-1.5h.Extract solution is merged.
Then, the second extract is prepared with the second Aqueous extracts.Preparing the second extract includes：After second Aqueous extracts are filtered
PH value is adjusted to 1-2, insulation is placed, filtering, collect precipitation, plus ethanol solution cleaning, then suction filtration, drying obtains the second extraction
Thing.It should be noted that " insulation is placed " herein, refers to the second Aqueous extracts obtained after radix scutellariae is decocted in the second Aqueous extracts
Insulation placement is carried out at intrinsic temperature, temperature is typically within the scope of 90-100 DEG C, no more than 100 DEG C.
Preferably, the second Aqueous extracts after filtering are adjusted into pH value to 1-2 with hydrochloric acid solution.
Preferably, precipitation is cleaned with ethanol solution 2-3 times.
Second extract can be understood as Baical Skullcap root P.E.
The embodiment of the present invention is decocted radix scutellariae in weakly alkaline solution, so that the scutelloside for being slightly soluble in water be extracted
Come, improve the content of active ingredient scutelloside in product, and then improve product curative effect.
4th, mix
By total volatile oil, the first extract and the second extract mix, obtain containing scutelloside, chlorogenic acid, galuteolin,
Forsythoside a, (R, S)-announcement are according to spring and the Chinese medicine composition of total volatile oil.And scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are (150-600) according to the weight ratio of spring and total volatile oil：(10-60)：(1-6)：(10-50)：
(2-9)：(50-200).
Main component is preferentially volatile honeysuckle oil, Forsythia volatile oil and Radix Isatidis by the preparation method of the embodiment of the present invention
The total volatile oil for playing oil is individually extracted, it is to avoid because directly causing volatile oils to lose medicinal material decocting, so that
The content of volatile oil in product is improved, and then improves the content of product active ingredient.
3rd aspect of the embodiment of the present invention is there is provided a kind of pharmaceutical preparation with heat-clearing toxin-expelling functions, and it is by upper
Chinese medicine composition and pharmaceutically acceptable carrier or the diluent composition stated.
Preferably, pharmaceutical preparation is mixture, syrup, tablet, granule or capsule.
It is highly preferred that pharmaceutical preparation is mixture.Mixture specification is 6 mls/branch, 10 mls/branch, 12 mls/branch or 20 millis
Rise/.Preparing the method for mixture includes：Chinese medicine composition is dissolved in water, add account for medicinal material total amount 2-10% sucrose or
Person 0.2-1% Steviosin, mixing makes dissolving, adds water to ormal weight, boils 10-20min, when temperature is down to below 80 DEG C
PH value is adjusted to 6-7.Then, filter, filling, sterilizing is produced.
With reference to embodiment, the present invention is further described.
Embodiment 1
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring, total volatile oil and forsythin, and weight ratio is 560：12：1：11：6：52：6.Above-mentioned Chinese traditional medicine composition
Thing is prepared from according to following preparation method：
400 parts of the honeysuckle counted by weight, 600 parts of water distillations in 3 times of amounts of 800 parts of the capsule of weeping forsythia and Radix Isatidis are carried
Take 1 time, extraction time is 5h.Extraction is obtained into distillate to distill again, rectifying liquid is obtained, rectifying liquid is to include honeysuckle volatilization
Oil, Forsythia volatile oil and Radix Isatidis play the total volatile oil of oil.Collect the aqueous solution obtained after distillation and filter standby, the dregs of a decoction are standby
With.According to weight ratio it is 1 by total volatile oil and cyclodextrin：5 ratio temperature be 30 DEG C, mixing time be to enter under conditions of 8h
Row inclusion, is then dried.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 1 time, are decocted
The time is boiled for 2h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount, standing and filter in stirring, collect supernatant and reclaim ethanol to solution without ethanol flavor, obtain
First extract.
600 parts of radix scutellariae is subjected to decocting extraction in pH value is 8 water, extracted 2 times, decocting time is respectively 1h, is obtained
To the second extract solution.It is 2 that salt acid for adjusting pH value is used after second extract solution is filtered, and insulation is placed, filtering, collection precipitation, plus second
Alcohol is washed 2 times, suction filtration, is dried, is obtained the second extract.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is syrup in the present embodiment.
Embodiment 2
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring, total volatile oil and forsythin, and weight ratio is 180：36：3.3：21：3.9：97：17.
Above-mentioned Chinese medicine composition is prepared from according to following preparation method：
By 500 parts of the honeysuckle counted by weight, 500 parts of water distillations in 8 times of amounts of 1000 parts of the capsule of weeping forsythia and Radix Isatidis
Extract 3 times, each extraction time is 3h.Extraction is obtained into distillate to merge, distilled again, rectifying liquid is obtained, rectifying liquid is bag
Include the total volatile oil that volatile honeysuckle oil, Forsythia volatile oil and Radix Isatidis play oil.Collect the aqueous solution filtering obtained after distillation
Standby, the dregs of a decoction are standby.According to weight ratio it is 1 by total volatile oil and cyclodextrin：15 ratio temperature be 40 DEG C, mixing time be
Included, then dried under conditions of 5h.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 1 time, are decocted
The time is boiled for 2h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount, standing and filter in stirring, collect supernatant and reclaim ethanol to solution without ethanol flavor, obtain
First extract.
300 parts of radix scutellariae is subjected to decocting extraction in pH value is 7.5 water, extracted 2 times, decocting time is respectively 1h,
Obtain the second extract solution.It is 2 that salt acid for adjusting pH value is used after second extract solution is filtered, and insulation is placed, filtering, collection precipitation, plus
Ethanol is washed 3 times, suction filtration, is dried, is obtained the second extract.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is mixture in the present embodiment.
The method for preparing mixture is：Chinese medicine composition is dissolved in water, the sucrose for accounting for medicinal material total amount 5% is added, mixing makes
Dissolving, is added water to ormal weight, boils 15min, and pH value is adjusted when temperature is down to below 80 DEG C to 6.Then, filter, it is filling,
Sterilizing, is produced.
Embodiment 3
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring, total volatile oil and forsythin, and weight ratio is 380：52：4：35：2：182：26.Above-mentioned Chinese medicine group
Compound is prepared from according to following preparation method：
By 600 parts of the honeysuckle counted by weight, 400 parts of water distillations in 10 times of amounts of 1200 parts of the capsule of weeping forsythia and Radix Isatidis
Extract 2 times, each extraction time is 2h.Extraction is obtained into distillate to merge, distilled again, rectifying liquid is obtained, rectifying liquid is bag
Include the total volatile oil that volatile honeysuckle oil, Forsythia volatile oil and Radix Isatidis play oil.Collect the aqueous solution filtering obtained after distillation
Standby, the dregs of a decoction are standby.According to weight ratio it is 1 by total volatile oil and cyclodextrin：10 ratio temperature be 50 DEG C, mixing time be
Included, then dried under conditions of 4h.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are carried out into decocting to extract 1 time, during decoction
Between be 2h, obtain Aqueous extracts.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 2 times, are decocted
The time is boiled for 1h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount, standing and filter in stirring, collect supernatant and reclaim ethanol to solution without ethanol flavor, obtain
First extract.
400 parts of radix scutellariae is subjected to decocting extraction in pH value is 8 water, extracted 2 times, decocting time is respectively 1h, is obtained
To the second extract solution.It is 2 that salt acid for adjusting pH value is used after second extract solution is filtered, and insulation is placed, filtering, collection precipitation, plus second
Alcohol is washed 2 times, suction filtration, is dried, is obtained the second extract.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is mixture in the present embodiment.
The method for preparing mixture is：Chinese medicine composition is dissolved in water, the Steviosin for accounting for medicinal material total amount 0.6% is added,
Mixing makes dissolving, adds water to ormal weight, boils 20min, and pH value 7 is adjusted when temperature is down to below 80 DEG C.Then, filter, fill
Dress, sterilizing, is produced.
Embodiment 4
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring, total volatile oil and forsythin, and weight ratio is 510：38：3.5：27：3.7：135：21.In above-mentioned
Drug composition is prepared from according to following preparation method：
By 500 parts of the honeysuckle counted by weight, 500 parts of water distillations in 12 times of amounts of 1000 parts of the capsule of weeping forsythia and Radix Isatidis
Extract 3 times, each extraction time is 1h.Extraction is obtained into distillate to merge, distilled again, rectifying liquid is obtained, rectifying liquid is bag
Include the total volatile oil that volatile honeysuckle oil, Forsythia volatile oil and Radix Isatidis play oil.Collect the aqueous solution filtering obtained after distillation
Standby, the dregs of a decoction are standby.According to weight ratio it is 1 by total volatile oil and cyclodextrin：12 ratio temperature be 30 DEG C, mixing time be
Included, then dried under conditions of 8h.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 1 time, are decocted
The time is boiled for 2h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount, standing and filter in stirring, collect supernatant and reclaim ethanol to solution without ethanol flavor, obtain
First extract.
500 parts of radix scutellariae is subjected to decocting extraction in pH value is 8 water, extracted 2 times, decocting time is respectively 1h, is carried
Take liquid filter after with salt acid for adjusting pH value be 2, insulation is placed, filtering, collects precipitation, plus ethanol washes 2 times, suction filtration, is dried, and obtains the
Two extracts.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is mixture in the present embodiment.
The method for preparing mixture is：Chinese medicine composition is dissolved in water, the Steviosin for accounting for medicinal material total amount 1% is added, mixes
Make dissolving, add water to ormal weight, boil 10min, pH value is adjusted when temperature is down to below 80 DEG C to 6.5.Then, filter,
Filling, sterilizing is produced.
Embodiment 5
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring and total volatile oil, and weight ratio is 550：50：3.9：16：2.5：86.Above-mentioned Chinese medicine composition be by
It is prepared from according to following preparation method：
600 parts of the honeysuckle counted by weight, 400 parts of water distillations in 5 times of amounts of 800 parts of the capsule of weeping forsythia and Radix Isatidis are carried
Take 1 time, extraction time is 5h.Extraction is obtained into distillate to distill again, rectifying liquid is obtained, rectifying liquid is to include honeysuckle volatilization
Oil, Forsythia volatile oil and Radix Isatidis play the total volatile oil of oil.Collect the aqueous solution obtained after distillation and filter standby, the dregs of a decoction are standby
With.According to weight ratio it is 1 by total volatile oil and cyclodextrin：5 ratio temperature be 30 DEG C, mixing time be to enter under conditions of 8h
Row inclusion, is then dried.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 1 time, are decocted
The time is boiled for 2h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount (can also be ethanol solution), standing and filter in stirring, collect supernatant and reclaim ethanol extremely
Solution obtains the first extract without ethanol flavor.
600 parts of radix scutellariae is subjected to decocting extraction in pH value is 8 water, extracted 2 times, decocting time is respectively 1h, is obtained
To the second extract solution.It is 2 that salt acid for adjusting pH value is used after second extract solution is filtered, and insulation is placed, filtering, collection precipitation, plus second
Alcohol is washed 2 times, suction filtration, is dried, is obtained the second extract.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is syrup in the present embodiment.
Embodiment 6
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring and total volatile oil, and weight ratio is 220：28：2.1：36：5.1：139.Above-mentioned Chinese medicine composition is
It is prepared from according to following preparation method：
By 400 parts of the honeysuckle counted by weight, 600 parts of water distillations in 5 times of amounts of 1200 parts of the capsule of weeping forsythia and Radix Isatidis
Extract 1 time, extraction time is 5h.Extraction is obtained into distillate to distill again, rectifying liquid is obtained, rectifying liquid is to include honeysuckle to wave
Hair oil, Forsythia volatile oil and Radix Isatidis play the total volatile oil of oil.Collect the aqueous solution obtained after distillation and filter standby, the dregs of a decoction are standby
With.According to weight ratio it is 1 by total volatile oil and cyclodextrin：5 ratio temperature be 30 DEG C, mixing time be to enter under conditions of 8h
Row inclusion, is then dried.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 1 time, are decocted
The time is boiled for 2h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount (can also be ethanol solution), standing and filter in stirring, collect supernatant and reclaim ethanol extremely
Solution obtains the first extract without ethanol flavor.
300 parts of radix scutellariae is subjected to decocting extraction in pH value is 8 water, extracted 2 times, decocting time is respectively 1h, is obtained
To the second extract solution.It is 2 that salt acid for adjusting pH value is used after second extract solution is filtered, and insulation is placed, filtering, collection precipitation, plus second
Alcohol is washed 2 times, suction filtration, is dried, is obtained the second extract.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is granule in the present embodiment.
Embodiment 7
The Chinese medicine composition with heat-clearing toxin-expelling functions of the present embodiment contains scutelloside, chlorogenic acid, galuteolin, the capsule of weeping forsythia
Ester glycosides a, (R, S)-announcement are according to spring and total volatile oil, and weight ratio is 360：41：3.2：26：5：125.Above-mentioned Chinese medicine composition be by
It is prepared from according to following preparation method：
By 500 parts of the honeysuckle counted by weight, 500 parts of water distillations in 3 times of amounts of 1000 parts of the capsule of weeping forsythia and Radix Isatidis
Extract 1 time, extraction time is 5h.Extraction is obtained into distillate to distill again, rectifying liquid is obtained, rectifying liquid is to include honeysuckle to wave
Hair oil, Forsythia volatile oil and Radix Isatidis play the total volatile oil of oil.Collect the aqueous solution obtained after distillation and filter standby, the dregs of a decoction are standby
With.According to weight ratio it is 1 by total volatile oil and cyclodextrin：5 ratio temperature be 30 DEG C, mixing time be to enter under conditions of 8h
Row inclusion, is then dried.
The dregs of a decoction of the dregs of a decoction of honeysuckle after distillation, the dregs of a decoction of the capsule of weeping forsythia, Radix Isatidis are subjected to decocting extraction, extracted 1 time, are decocted
The time is boiled for 2h, the first Aqueous extracts are obtained.After the aqueous solution and the first Aqueous extracts that are obtained when distilling and extracting are filtered, concentrated respectively
Merge, add after ethanol in proper amount (can also be ethanol solution), standing and filter in stirring, collect supernatant and reclaim ethanol extremely
Solution obtains the first extract without ethanol flavor.
450 parts of radix scutellariae is subjected to decocting extraction in pH value is 8 water, extracted 2 times, decocting time is respectively 1h, is obtained
To the second extract solution.It is 2 that salt acid for adjusting pH value is used after second extract solution is filtered, and insulation is placed, filtering, collection precipitation, plus second
Alcohol is washed 2 times, suction filtration, is dried, is obtained the second extract.
Total volatile oil, the first extract and the second extract are mixed, Chinese medicine composition is obtained.
Obtained above-mentioned Chinese medicine composition and pharmaceutically acceptable carrier or diluent are prepared into clearing heat and detoxicating
The pharmaceutical preparation of effect.Pharmaceutical preparation is capsule in the present embodiment.
Comparative example
500 parts of radix scutellariae, 500 parts of honeysuckle, 1000 parts of the capsule of weeping forsythia, 500 parts of Radix Isatidis are measured, 500 parts of radix scutellariae plus 8 times of decoctings are boiled
Three times, 2 hours for the first time, second and third time each 1 hour, collecting decoction, and filtration, filtrate concentrates and adds 2mol/L at 80 DEG C
Hydrochloric acid solution adjusts pH value to 1.2 in right amount, is incubated 1 hour, stands 12 hours, filtration, precipitation plus 6 times of amount water, uses 40% hydrogen-oxygen
Change sodium solution and adjust pH value to 7.0, then add equivalent ethanol, be stirred to dissolve, filter, filtrate adjusts pH with 2mo1/L hydrochloric acid solutions
It is worth to 2.0,60 DEG C and is incubated 30 minutes, stands 12 hours, filtration, it is 7.0 that precipitation, which is washed till pH value with ethanol, and recovery ethanol is standby；
After 500 parts of honeysuckle, 1000 parts of the capsule of weeping forsythia, 500 parts plus 8 times water temperatures of Radix Isatidis are soaked 30 minutes, secondary, 1.5 hours every time, conjunction are decocted
And decocting liquid, filter, filtrate is concentrated into the clear cream that relative density is 1.22 (70 DEG C), is slowly added into ethanol when being cooled to 40 DEG C, makes to contain
Alcohol amount is sufficiently stirred for up to 75%, stands 12 hours, leaching supernatant, and residue adds the suitable child of 75% ethanol, stirs evenly, stands 12 hours,
Filtration, merges ethanol, reclaims ethanol to without alcohol taste, plus the above-mentioned Baical Skullcap root P.E of people, obtains extract i.e. Comparative composition.
Below by experiment to the Chinese medicine composition of above-described embodiment and the active drug of the Comparative composition of comparative example into
Divide and measure.
Experimental method：
1st, the assay of scutelloside
Determined according to high performance liquid chromatography (Chinese Pharmacopoeia version general rule 0512 in 2015).
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；Using acetonitrile as flowing
Phase A, 0.1% phosphoric acid solution is that the regulation in Mobile phase B, according to the form below 1 carries out gradient elution：Scutelloside Detection wavelength 274nm.Reason
6000 should be not less than by being calculated by plate number by peak.
Table 1
" → " represents the change of flowing phase concentration in table.
The preparation of reference substance solution：Take scutelloside reference substance appropriate, it is accurately weighed, plus every lml is respectively prepared in 50% methanol
The solution of the μ g containing scutelloside 60, is produced.
The preparation of need testing solution：Precision weighs each 100mg of embodiment 1-7 and comparative example Chinese medicine composition, puts respectively
In 100ml measuring bottles, plus 50% methanol 90ml, ultrasonically treated (power 250W, frequency 25kHz) 30 minutes is let cool, plus 50% first
Alcohol shakes Uniform to scale, filtration, takes subsequent filtrate, produces.
Assay method：It is accurate respectively to draw reference substance solution and each 10 μ l of need testing solution, liquid chromatograph is injected, is surveyed
It is fixed, produce.
2nd, the assay of chlorogenic acid
Determined according to high performance liquid chromatography (Chinese Pharmacopoeia version general rule 0512 in 2015).
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；With methanol-water-
Glacial acetic acid-triethylamine (15：85：1：0.3) it is mobile phase；Detection wavelength is 324nm.Number of theoretical plate is calculated by chlorogenic acid peak should
It is not less than 6000.
The preparation of reference substance solution：Take chlorogenic acid reference substance appropriate, it is accurately weighed, put in brown measuring bottle, adding water, it is every to be made
1ml contains 20 μ g solution, produces.
The preparation of need testing solution：Precision weighs each 200mg of embodiment 1-7 and comparative example Chinese medicine composition, puts respectively
In 100ml measuring bottles, plus 70% methanol 90ml, ultrasonically treated (power 250W, frequency 25kHz) 30 minutes is let cool, plus 70% first
Alcohol shakes up to scale, filtration, takes subsequent filtrate, produces.
Assay method：It is accurate respectively to draw reference substance solution and each 10 μ l of need testing solution, liquid chromatograph is injected, is surveyed
It is fixed, produce.
3rd, the assay of galuteolin
Determined according to high performance liquid chromatography (Chinese Pharmacopoeia version general rule 0512 in 2015).
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；With second eyeball -0.2%
Phosphoric acid solution (20：80) it is mobile phase；Detection wavelength is 350nm.Number of theoretical plate is calculated by galuteolin peak should be not less than 3000.
The preparation of reference substance solution：Take galuteolin reference substance appropriate, it is accurately weighed, plus every 1ml is made containing 20 μ g in methanol
Solution, produce.
The preparation of need testing solution：Precision weighs each 1g of embodiment 1-7 and comparative example Chinese medicine composition, is respectively placed in
In 25ml measuring bottles, plus 70% methanol 20ml, ultrasonically treated (power 250W, frequency 25kHz) 30 minutes is let cool, plus 70% methanol
To scale, shake up, filter, take subsequent filtrate, produce.
Determination method is accurate respectively to draw reference substance solution and each 20 μ l of need testing solution, notes people's liquid chromatograph, determines, i.e.,
.
4th, Forsythoside a assay
Determined according to high performance liquid chromatography (Chinese Pharmacopoeia version general rule 0512 in 2015).
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；With second eyeball -0.4%
Glacial acetic acid solution (15：85) it is mobile phase；Detection wavelength is 330nm.Number of theoretical plate is calculated by capsule of weeping forsythia vinegar former times A peaks to be not less than
5000。
The preparation of reference substance solution：Take Forsythoside a reference substances appropriate, it is accurately weighed, plus methanol is made every 1ml and contains
0.1mg solution, produces and (faces with preparation).
The preparation of need testing solution：Precision weighs embodiment 1-7 and the composition 500mg of comparative example, puts 25ml measuring bottles
In, plus 70% methanol 20ml, ultrasonically treated (power 250W, frequency 25kHz) 30 minutes is let cool, plus 70% methanol shakes to scale
Even, filtration takes subsequent filtrate, produced.
Assay method：It is accurate respectively to draw reference substance solution and each 10 μ l of need testing solution, liquid chromatograph is injected, is surveyed
It is fixed, produce.
5th, (R, S)-accuse according to the spring assay
Determined according to high performance liquid chromatography (Chinese Pharmacopoeia version general rule 0512 in 2015).
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；With methanol-
0.02% phosphoric acid solution (7：93) it is mobile phase；Detection wavelength 245nm.Number of theoretical plate is calculated by (R, S)-announcement according to spring peak should not
Less than 5000.
The preparation of reference substance solution：(R, S)-accuse appropriate according to spring reference substance is taken, it is accurately weighed, plus methanol is made every 1ml and contains
20 μ g solution, is produced.
The preparation of need testing solution：Precision weighs embodiment 1-7 and the composition 1g of comparative example, puts in 25ml measuring bottles, essence
Close addition water 20m1, ultrasonically treated (power 250W, frequency 25kHz) 30 minutes lets cool, the weight of less loss is supplied with water, is shaken up,
Filtration, takes subsequent filtrate, produces.
Determination method：It is accurate respectively to draw reference substance solution and each 20 μ l of need testing solution, liquid chromatograph is injected, is determined,
Produce.
6th, the assay of forsythin
Determined according to high performance liquid chromatography (Chinese Pharmacopoeia version general rule 0512 in 2015).
Chromatographic condition and system suitability：Using octadecylsilane chemically bonded silica as filler；Using acetonitrile as flowing
Phase A, using water as Mobile phase B, the regulation according to the form below 2 carries out gradient elution；Detection wavelength is 229nm.Number of theoretical plate presses the capsule of weeping forsythia
Glycosides peak, which is calculated, should be not less than 3000.
Table 2
The preparation of reference substance solution：Take forsythin reference substance, it is accurately weighed, plus methanol be made every lml contain 15 μ g solution,
Produce.
The preparation of need testing solution：Precision weighs each 100mg of embodiment 1-7 and comparative example composition, is respectively placed in
In 25ml measuring bottles, plus methanol 20ml, ultrasonically treated (power 250W, frequency 25kHz) 30 minutes is let cool, plus methanol is to scale, shakes
Even, filtration takes subsequent filtrate, produced.
Assay method：It is accurate respectively to draw reference substance solution and each 10 μ l of need testing solution, liquid chromatograph is injected, is surveyed
It is fixed, produce.
7th, the assay of total volatile oil
Determined according to determination of volatile oil method (the Chinese Pharmacopoeia first method of version general rule 2204 in 2015).
Precision weighs each 20g of embodiment 1-7 and comparative example composition, puts in flask, the 300-500m1 that adds water (or it is suitable
Amount) and bead number, after shaking mixing, connection volatile oil determination apparatus and reflux condensing tube.Autocondensation pipe upper end adds water and makes to fill
The scale part of full volatile oil determination apparatus, and untill overflow is when entering flask.Put in electric jacket or slowly added with other proper methods
Heat keeps micro-boiling about 5 hours to boiling, and into analyzer, oil mass is not further added by, and stops heating, places a moment, unlatching analyzer
The piston of lower end, water is slowly released, untill above the oil reservoir upper end arrival line of scale 0 at 5mm.Place more than 1 hour, then open
Opening piston makes oil reservoir drop to its upper end just and scale.Line is concordant, reads volatilization oil mass, and calculates containing for volatile oil in test sample
Measure (%).
Experimental result is collected to table 3.
3 active constituent contents of table/mg (every gram of composition)
" -- " represents that reality is not detected.
From table 3 it can be seen that embodiments of the invention 1-7 is for comparative example, it is identical (real in medicinal material inventory
Apply example 4 and comparative example) in the case of, the content of various active ingredients is significantly higher than comparative example, even if the content of various active ingredients
Comparative example, especially total volatile oil are above in addition to scutelloside in the case where medicinal material inventory is slightly less than comparative example, it has
More preferably therapeutic effect.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (10)
1. a kind of Chinese medicine composition with heat-clearing toxin-expelling functions, it is characterised in that the Chinese medicine composition contains scutelloside, green
Ortho acid, galuteolin, Forsythoside a, (R, S)-announcement are according to spring and total volatile oil, the scutelloside, the chlorogenic acid, the wood
Rhinoceros grass glycosides, the Forsythoside a, (R, the S)-announcement are (150-600) according to the weight ratio of spring and the total volatile oil：
(10-60)：(1-6)：(10-50)：(2-9)：(50-200), wherein, the total volatile oil is by honeysuckle, the capsule of weeping forsythia and plate
Blue root is extracted and formed.
2. the Chinese medicine composition according to claim 1 with heat-clearing toxin-expelling functions, it is characterised in that the Chinese traditional medicine composition
Thing also includes forsythin；
Preferably, the Chinese medicine composition contains the scutelloside, the chlorogenic acid, the galuteolin, the capsule of weeping forsythia ester
Glycosides a, (R, the S)-announcement are (180-560) according to the weight ratio in spring, the total volatile oil and the forsythin：(12-52)：
(1-7)：(11-35)：(2-6)：(52-182)：(6-26).
3. a kind of preparation method of the Chinese medicine composition as claimed in claim 1 or 2 with heat-clearing toxin-expelling functions, its feature exists
In, including：
By 400-600 parts of 400-600 parts of the honeysuckle counted by weight, 800-1200 parts of the capsule of weeping forsythia and Radix Isatidis in 3-12
The water distillation of amount is extracted again, and extraction is obtained into distillate and distilled again, obtains rectifying liquid, and the rectifying liquid is total volatilization
Oil；
The dregs of a decoction of the dregs of a decoction, the dregs of a decoction of the capsule of weeping forsythia and the Radix Isatidis of the honeysuckle after distillation are subjected to decocting extraction,
Obtain the first Aqueous extracts；The aqueous solution obtained when being extracted with first Aqueous extracts with distillation prepares the first extract；
300-600 parts of the radix scutellariae counted by weight is subjected to decocting extraction in pH value is 7-8 water, the second water extraction is obtained
Liquid, the second extract is prepared with second Aqueous extracts；And by the total volatile oil, first extract and described second
Extract mix, obtain containing the scutelloside, the chlorogenic acid, the galuteolin, the Forsythoside a, it is described (R,
S)-accuse according to spring and the Chinese medicine composition of the total volatile oil, and the scutelloside, the chlorogenic acid, the reseda
Glycosides, the Forsythoside a, (R, the S)-announcement are (150-600) according to the weight ratio of spring and the total volatile oil：(10-
60)：(1-6)：(10-50)：(2-9)：(50-200).
4. the preparation method of the Chinese medicine composition according to claim 3 with heat-clearing toxin-expelling functions, it is characterised in that institute
Stating preparation method also includes：The total volatile oil and cyclodextrin are included, then dried.
5. the preparation method of the Chinese medicine composition according to claim 4 with heat-clearing toxin-expelling functions, it is characterised in that will
The total volatile oil is 1 according to weight ratio with the cyclodextrin：The ratio of (5-20) is 30-60 DEG C, mixing time in temperature
To be included under conditions of 1-8h.
6. the preparation method of the Chinese medicine composition with heat-clearing toxin-expelling functions according to any one of claim 3-5, its
It is characterised by, preparing first extract includes：The aqueous solution obtained when first Aqueous extracts and distillation are extracted
Filter, concentrate respectively, be then combined with, stir, add after ethanol solution, standing and filter in whipping process, collect supernatant simultaneously
Ethanol is reclaimed to solution without ethanol flavor, first extract is obtained.
7. the Chinese medicine composition with heat-clearing toxin-expelling functions according to any one of claim 3-5, it is characterised in that
Preparing second extract includes：Regulation pH value is to 1-2 after second Aqueous extracts are filtered, and insulation is placed, and is filtered, and is received
Collection precipitation, plus ethanol solution cleaning, then suction filtration, drying obtain second extract.
8. a kind of pharmaceutical preparation with heat-clearing toxin-expelling functions, it is characterised in that as the Chinese traditional medicine composition described in claim 1 or 2
Thing and pharmaceutically acceptable carrier or diluent composition.
9. the pharmaceutical preparation according to claim 8 with heat-clearing toxin-expelling functions, it is characterised in that the pharmaceutical preparation is
Mixture, syrup, tablet, granule or capsule.
10. the pharmaceutical preparation according to claim 9 with heat-clearing toxin-expelling functions, it is characterised in that when the medicine system
When agent is mixture, mixture specification is 6 mls/branch, 10 mls/branch, 12 mls/branch or 20 mls/branch, prepares the side of the mixture
Method includes：The Chinese medicine composition is dissolved in water, the sweet tea of the sucrose or 0.2-1% that account for medicinal material total amount 2-10% is added
Chrysanthemum element, mixing makes dissolving, adds water to ormal weight, boils 10-20min, and pH value is adjusted when temperature is down to below 80 DEG C to 6-
7, then filtration, filling, sterilizing, are produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710286080.5A CN107126465A (en) | 2017-04-26 | 2017-04-26 | A kind of Chinese medicine composition with heat-clearing toxin-expelling functions and preparation method thereof and pharmaceutical preparation |
Applications Claiming Priority (1)
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| CN111297880A (en) * | 2019-12-13 | 2020-06-19 | 北京中医药大学 | A Chinese medicinal composition for inhibiting tumor proliferation and migration |
| CN112557526A (en) * | 2020-11-16 | 2021-03-26 | 成都市食品药品检验研究院 | Quality-controllable isatis root extract and method for identifying isatis root containing raw material medicine in Chinese patent medicine |
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Application publication date: 20170905 |