CN107788118A - A kind of functional yoghourt produced using subbacterial - Google Patents

A kind of functional yoghourt produced using subbacterial Download PDF

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Publication number
CN107788118A
CN107788118A CN201710701561.8A CN201710701561A CN107788118A CN 107788118 A CN107788118 A CN 107788118A CN 201710701561 A CN201710701561 A CN 201710701561A CN 107788118 A CN107788118 A CN 107788118A
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CN
China
Prior art keywords
subbacterial
yoghourt
streptococcus thermophilus
plant
produced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710701561.8A
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Chinese (zh)
Inventor
龄南
陈萍
王红兵
陈晓霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Weigang Dairy Enterprise Co ltd
Original Assignee
Nanjing Weigang Dairy Enterprise Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Weigang Dairy Enterprise Co ltd filed Critical Nanjing Weigang Dairy Enterprise Co ltd
Priority to CN201710701561.8A priority Critical patent/CN107788118A/en
Publication of CN107788118A publication Critical patent/CN107788118A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)

Abstract

A kind of functional yoghourt produced using subbacterial of the present invention, by one plant of lactobacillus bulgaricus and two plants of streptococcus thermophiluses be mixed in proportion fermentation form and make work seed carry out Yoghourt Production, on the premise of ensureing that the production time is constant, it is therefore prevented that due to the too fast quality problems for producing whey and separating out of fermenting.Wherein lactobacillus bulgaricus has certain anti-pathogenic bacteria effect simultaneously, has certain effect to intestinal microecology balance, exocellular polysaccharide caused by streptococcus thermophilus also has certain immunization.These comprehensive bacterial strain features so that also there is certain prebiotic function by the Yoghourt of mixing subbacterial production.

Description

A kind of functional yoghourt produced using subbacterial
Technical field
The present invention relates to a kind of functional yoghourt produced using subbacterial.
Background technology
Subbacterial fermenting and producing Yoghourt is very traditional Yoghourt Production mode, has the characteristics of fermenting speed is fast.After One plant of lactobacillus bulgaricus and one plant of streptococcus thermophilus are used with 1 for strain:1 proportions are fermented, it is therefore an objective to are Kept relative stability in co-passage incubation, so as to ensureing the stability of product.But this method is even utilized, It can produce due to too fast the problem of causing whey precipitation serious of fermenting.The strain that works simultaneously substantially produces fermentation, does not exist Function affect is produced while fermentation.
The content of the invention
To solve the defects of prior art is present, the present invention provides a kind of functional yoghourt produced using subbacterial, It is to be mixed fermentation in proportion by one plant of lactobacillus bulgaricus and two plants of streptococcus thermophiluses to form.
Further, it is with certain immunization by one plant of lactobacillus bulgaricus with bacteria resistance function and one plant Streptococcus thermophilus and the excellent streptococcus thermophilus of the viscous effect of one plant of production be mixed fermentation in proportion and form.
Further, the streptococcus thermophilus with certain immunization is streptococcus thermophilus WG02, and Classification And Nomenclature is:It is thermophilic Streptococcus Streptococcus thermophilus;The biomaterial was preserved in China Microbiological on 03 08th, 2017 Culture presevation administration committee common micro-organisms center, preserving number are CGMCC No.13738, and specific address is exposed to the sun for Beijing No. 3 Institute of Microorganism, Academia Sinica of institute of area North Star West Road 1.
Further, it is prepared by the method for following steps:
S1, subbacterial preparation process
S11, by the lactobacillus bulgaricus with bacteria resistance function with 2% connect bacterium amount access 12% sterilized non-fat recombined milk In, 37 DEG C of activation 16h, 4 DEG C of storage more than 6h after taking-up;
S12, by the streptococcus thermophilus with certain immunization with 2% connect bacterium amount access 12% sterilized non-fat recombined milk In, 37 DEG C of activation 16h, 4 DEG C of storage more than 6h after taking-up;
S13, the excellent streptococcus thermophilus of viscous effect will be produced connect bacterium amount with 2% and access in 12% sterilized non-fat recombined milk, 37 DEG C activation 16h, after taking-up 4 DEG C storage more than 6h;
S14, by the single strain obtained by step S11, S12, S13 according to 1:1:0.1 ratio is mixed, after mixing with 3%, which connects bacterium amount, makes work seed;
S2, acidified milk preparation process
S21,900 parts of fresh cow milk is heated to 60 DEG C, adds 70 parts of sucrose, stirring material 20min;
S22, the mixed liquor that step (1) obtains is subjected to homogeneous, 20-25 MPas of homogenization pressure under the conditions of 60 DEG C;
S23, the mixed liquor for obtaining step (2) pasteurization 300s under the conditions of 95 DEG C, and it is cooled to 42 DEG C;
S24, the sterilizing mixed liquor for obtaining step (3) access subbacterial, and the 3.5h that fermented under the conditions of 42 DEG C, fermentation The Yoghourt fermented is inserted into after-ripening 12h in 4 DEG C of environment after end and produces described solidification type yoghourt.
Beneficial effect:
The present invention has carried out more bacterial strain compoundings to subbacterial, has used one plant of lactobacillus bulgaricus and two plants of thermophilic chains Coccus carries out squamous subculture, and makes work seed and carry out Yoghourt Production, on the premise of ensureing that the production time is constant, it is therefore prevented that Due to the too fast quality problems for producing whey and separating out of fermenting.
Wherein one plant of lactobacillus bulgaricus has certain anti-pathogenic bacteria effect simultaneously, has one to intestinal microecology balance Determine effect.Exocellular polysaccharide caused by one plant of streptococcus thermophilus also has certain immunization.These comprehensive bacterial strain features, make Obtain also has certain prebiotic function by the Yoghourt of mixing subbacterial production.
Embodiment
A kind of functional yoghourt produced using subbacterial, it is by one plant of lactobacillus bulgaricus and two plants of thermophilic chains Coccus is mixed fermentation and formed in proportion.
Comprise the following steps that:
S1, subbacterial preparation process
S11, by the lactobacillus bulgaricus with bacteria resistance function with 2% connect bacterium amount access 12% sterilized non-fat recombined milk In, 37 DEG C of activation 16h, 4 DEG C of storage more than 6h after taking-up;
S12, by the streptococcus thermophilus WG02 with certain immunization with 2% connect bacterium amount access 12% sterilized non-fat also In former breast, 37 DEG C of activation 16h, 4 DEG C of storage more than 6h after taking-up;
S13, the excellent streptococcus thermophilus of viscous effect will be produced connect bacterium amount with 2% and access in 12% sterilized non-fat recombined milk, 37 DEG C activation 16h, after taking-up 4 DEG C storage more than 6h;
S14, by the single strain obtained by step S11, S12, S13 according to 1:1:0.1 ratio is mixed, after mixing with 3%, which connects bacterium amount, makes work seed;
S2, acidified milk preparation process
S21,900 parts of fresh cow milk is heated to 60 DEG C, adds 70 parts of sucrose, stirring material 20min;
S22, the mixed liquor that step (1) obtains is subjected to homogeneous, 20-25 MPas of homogenization pressure under the conditions of 60 DEG C;
S23, the mixed liquor for obtaining step (2) pasteurization 300s under the conditions of 95 DEG C, and it is cooled to 42 DEG C;
S24, the sterilizing mixed liquor for obtaining step (3) access subbacterial, and the 3.5h that fermented under the conditions of 42 DEG C, fermentation The Yoghourt fermented is inserted into after-ripening 12h in 4 DEG C of environment after end and produces described solidification type yoghourt.
The present invention has carried out more bacterial strain compoundings to subbacterial, has used one plant of lactobacillus bulgaricus and two plants of thermophilic chains Coccus carries out squamous subculture, and makes work seed and carry out Yoghourt Production, on the premise of ensureing that the production time is constant, it is therefore prevented that Due to the too fast quality problems for producing whey and separating out of fermenting.
Wherein one plant of lactobacillus bulgaricus has certain anti-pathogenic bacteria effect simultaneously, has one to intestinal microecology balance Determine effect.Exocellular polysaccharide caused by one plant of streptococcus thermophilus also has certain immunization.These comprehensive bacterial strain features, make Obtain also has certain prebiotic function by the Yoghourt of mixing subbacterial production.

Claims (4)

  1. A kind of 1. functional yoghourt produced using subbacterial, it is characterised in that its be by one plant of lactobacillus bulgaricus and Two plants of streptococcus thermophiluses are mixed fermentation and formed in proportion.
  2. 2. the functional yoghourt produced as claimed in claim 1 using subbacterial, it is characterised in that it is that had by one plant The lactobacillus bulgaricus of bacteria resistance function and one plant of streptococcus thermophilus with certain immunization and the viscous effect of one plant of production are excellent Streptococcus thermophilus be mixed fermentation in proportion and form.
  3. 3. the streptococcus thermophilus according to claim 2 with certain immunization is streptococcus thermophilus WG02, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number are CGMCC No.13738.
  4. 4. the functional yoghourt according to claim 2 produced using subbacterial, it is characterised in that it is by following step Rapid method is prepared:
    S1, subbacterial preparation process
    S11, by the lactobacillus bulgaricus with bacteria resistance function with 2% connect bacterium amount access 12% sterilized non-fat recombined milk in, 37 DEG C activation 16h, after taking-up 4 DEG C storage more than 6h;
    S12, by the streptococcus thermophilus with certain immunization with 2% connect bacterium amount access 12% sterilized non-fat recombined milk in, 37 DEG C activation 16h, after taking-up 4 DEG C storage more than 6h;
    S13, the excellent streptococcus thermophilus of viscous effect will be produced connect bacterium amount with 2% and access in 12% sterilized non-fat recombined milk, 37 DEG C of work Change 16h, 4 DEG C of storage more than 6h after taking-up;
    S14, by the single strain obtained by step S11, S12, S13 according to 1:1:0.1 ratio is mixed, with 3% after mixing Connect bacterium amount and make work seed;
    S2, acidified milk preparation process
    S21,900 parts of fresh cow milk is heated to 60 DEG C, adds 70 parts of sucrose, stirring material 20min;
    S22, the mixed liquor that step (1) obtains is subjected to homogeneous, 20-25 MPas of homogenization pressure under the conditions of 60 DEG C;
    S23, the mixed liquor for obtaining step (2) pasteurization 300s under the conditions of 95 DEG C, and it is cooled to 42 DEG C;
    S24, the sterilizing mixed liquor for obtaining step (3) access subbacterial, and the 3.5h that fermented under the conditions of 42 DEG C, fermentation ends The Yoghourt fermented is inserted into after-ripening 12h in 4 DEG C of environment afterwards and produces described solidification type yoghourt.
CN201710701561.8A 2017-08-16 2017-08-16 A kind of functional yoghourt produced using subbacterial Pending CN107788118A (en)

Priority Applications (1)

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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710701561.8A CN107788118A (en) 2017-08-16 2017-08-16 A kind of functional yoghourt produced using subbacterial

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101176484A (en) * 2007-11-13 2008-05-14 山东得益乳业有限公司 Improved use method of sour milk subbacterial
CN104054822A (en) * 2014-06-26 2014-09-24 武汉光明乳品有限公司 Yogurt fermentative lactic bacteria combination and fermenting agent
CN105886434A (en) * 2016-05-05 2016-08-24 上海理工大学 Streptococcus thermophilus and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101176484A (en) * 2007-11-13 2008-05-14 山东得益乳业有限公司 Improved use method of sour milk subbacterial
CN104054822A (en) * 2014-06-26 2014-09-24 武汉光明乳品有限公司 Yogurt fermentative lactic bacteria combination and fermenting agent
CN105886434A (en) * 2016-05-05 2016-08-24 上海理工大学 Streptococcus thermophilus and application thereof

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Application publication date: 20180313