CN104544390A - Preparation method of litchi lactic acid bacteria drink by employing complex probiotic fermentation - Google Patents

Preparation method of litchi lactic acid bacteria drink by employing complex probiotic fermentation Download PDF

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Publication number
CN104544390A
CN104544390A CN201410752388.0A CN201410752388A CN104544390A CN 104544390 A CN104544390 A CN 104544390A CN 201410752388 A CN201410752388 A CN 201410752388A CN 104544390 A CN104544390 A CN 104544390A
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parts
preparation
litchi
sour milk
fermentation
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CN201410752388.0A
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Chinese (zh)
Inventor
张斌
薛子光
吴军
张天义
王春宁
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GUANGDONG ZHENZHOU GROUP CO Ltd
Huizhou University
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GUANGDONG ZHENZHOU GROUP CO Ltd
Huizhou University
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Priority to CN201410752388.0A priority Critical patent/CN104544390A/en
Publication of CN104544390A publication Critical patent/CN104544390A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/519Breve

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Dairy Products (AREA)

Abstract

The invention relates to a preparation method of litchi lactic acid bacteria drink by employing complex probiotic fermentation, and belongs to the field of preparation of the litchi lactic acid bacteria drink. The litchi lactic acid bacteria drink is prepared from the following components in parts by weight: 10-20 parts of concentrated litchi juice, 1-3 parts of lactobacillus bulgaricus, 2-4 parts of streptococcus thermophilus, 1-3 parts of bifidobacterium, 10-15 parts of xanthan gum, 5-10 parts of pectin, 10-15 parts of CMC, 10-20 parts of white granulated sugar and 5-15 parts of citric acid. The preparation process comprises the following steps: inoculating in the concentrated litchi juice; fermenting, and carrying out after-ripening; adding a stabilizer, liquid glucose, water and an acidulant; and breaking curdled milk, mixing accessories, homogenizing and carrying out sterile filling. The preparation method of the litchi lactic acid bacteria drink employing the complex probiotic fermentation is easy to operate; and the three kinds of probiotics such as the lactobacillus bulgaricus, the streptococcus thermophilus and the bifidobacterium are added, so that the human intestinal health is facilitated. In addition, the litchi concentrated juice is added according to the method, so that the litchi lactic acid bacteria drink is abundant in flavor, free of essence, natural and pure.

Description

A kind of preparation method of composite probiotics ferment lichee sour milk beverage
Technical field
The present invention relates to a kind of method of lichee lactic acid drink, specifically a kind of preparation method of composite probiotics ferment lichee sour milk beverage, belongs to lichee sour milk beverage preparing technical field.
Background technology
Sour milk beverage is yoghurt based products, has the feature of beverage, the nutrition containing milk and the function of yogurt, is also one of dairy processing industry product with fastest developing speed.Adopt the probio with health care to make sour milk beverage as leavening, impart the nutritive value that this kind of milk product is special.Make it improve digestive function in lifting intestines and stomach immunologic function " breeding of suppression growth of pathogenic bacteria " and " reduce the extensive concern causing consumer in plasma cholesterol concentration and even Tumor suppression generation etc.
Lactic acid bacteria in matzoon, classification belongs to Bacillus acidi lactici, because of features such as its bacterial classification place of production, microbiologic properties, usefulness excellences, by microbiologist's called after lactobacillus delbruockii subspecies bulgaricus (abbreviation lactobacillus bulgaricus).A kind of bacterium by titled with name of the country, and is produced a male heir to continue the family line, and procreation so far, is found everywhere through the world, and usefulness is excellent again, good health and a long life of helping others, and really must make us feeling mystery.Lactic acid bacteria is the general name of one group of bacterium, comprising galactococcus, sheet coccus, bright string coccus, lactobacillus (being wherein representative with lactobacillus bulgaricus) and Bifidobacterium.They have a common ability, exactly can sugar fermentation and obtain energy, produce a large amount of lactic acid.
Streptococcus thermophilus and Bu Shi lactobacillus are all the essential materials making Yoghourt.Research shows that this bacterium can only arrive the first half of small intestine, and can go to large intestine unlike Bifidobacterium.Scientist thinks that Yoghourt bacterium (streptococcus thermophilus and Bu Shi lactobacillus) alive can help the people of lactose intolerance to digest lactose because these two kinds of bacterium can produce can lactase.
The quantity that Bifidobacterium is distributed in stomach and intestine reduces with the growth of age level, and distributing maximum is breast milk nutrition youngster.Have been found that Bifidobacterium has 32 hypotypes, the biologic product containing Bifidobacterium reaches 70 kinds.Bifidobacterium infantis accounts for 60 percent of total enteron aisle bacterium, and within more than 60 years old, old man Bifidobacterium only accounts for 7 percent nine.
Chinese patent, the patent No.: CN 201410052789.5; A kind of viable type lactobacillus-fermented of the present invention litchi beverage and preparation method thereof, is rich in biodiasmin in litchi beverage prepared by the present invention; Its preparation method is: first obtained Lychee juice, then leaves standstill 2 ~ 3h toward wherein adding DMDC room temperature, then adjust ph is 6 ~ 7, adds lactobacillus-fermented, can obtain fermentation litchi beverage.The present invention DMDC at room temperature carries out sterilization processing to Lychee juice, avoids the destruction of traditional hot pasteurize to Lychee juice local flavor and nutrition, does not also affect the fermentation of lactic acid bacteria.But it does not adopt lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium composite fermentation.Therefore, develop a kind ofly adopt lactobacillus bulgaricus, streptococcus thermophilus, lichee sour milk beverage tool prepared by Bifidobacterium is of great significance.
Summary of the invention
The object of this invention is to provide a kind of preparation method of composite probiotics ferment lichee sour milk beverage, preparation technology is simple to operate for this composite probiotics ferment lichee sour milk beverage, with the addition of three kinds of probios: lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, be conducive to the intestinal health of people, in addition, this method adds lichee inspissated juice, and local flavor is pure, without the need to adding essence, natural pure.
Object of the present invention is achieved by the following technical programs:
A kind of composite probiotics ferment lichee sour milk beverage, is obtained by following composition:
Concentrated Lychee juice 10-20 part, lactobacillus bulgaricus 1-3 part, streptococcus thermophilus 2-4 part, Bifidobacterium 1-3 part, xanthans 10-15 part, pectin 5-10 part, CMC10-15 part, white granulated sugar 10-20 part, citric acid 5-15 part.
A preparation method for composite probiotics ferment lichee sour milk beverage, preparation technology is:
Inoculate in concentrated Lychee juice, fermentation, after-ripening, add stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling; Concrete steps are as follows:
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 2-5%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 38-48 DEG C, fermentation 20-40h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 3-6 DEG C of refrigerator and preserves 10-18h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
The present invention relative to the beneficial effect of prior art is:
Compared with additive method, preparation technology is simple to operate for this composite probiotics ferment lichee sour milk beverage, with the addition of three kinds of probios: lactobacillus bulgaricus, streptococcus thermophilus, Bifidobacterium, be conducive to the intestinal health of people, in addition, this method adds lichee inspissated juice, and local flavor is pure, without the need to adding essence, natural pure.
Different pH value is on the impact of product:
The microbiological indicator testing result of product:
Detailed description of the invention
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
Embodiment 1
A kind of composite probiotics ferment lichee sour milk beverage, is obtained by following composition:
Concentrated Lychee juice 10 parts, lactobacillus bulgaricus 1 part, streptococcus thermophilus 2 parts, Bifidobacterium 1 part, xanthans 10 parts, pectin 5 parts, CMC10 part, white granulated sugar 10 parts, citric acid 5 parts;
A preparation method for composite probiotics ferment lichee sour milk beverage, preparation technology is:
Inoculate in concentrated Lychee juice, fermentation, after-ripening, add stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling;
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 2%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 38 DEG C, fermentation 20h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 3 DEG C of refrigerators and preserves 10h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
Embodiment 2
A kind of composite probiotics ferment lichee sour milk beverage, is obtained by following composition:
Concentrated Lychee juice 15 parts, lactobacillus bulgaricus 1 part, streptococcus thermophilus 3 parts, Bifidobacterium 1 part, xanthans 15 parts, pectin 6 parts, CMC11 part, white granulated sugar 12 parts, citric acid 7 parts;
A preparation method for composite probiotics ferment lichee sour milk beverage, preparation technology is:
Inoculate in concentrated Lychee juice, fermentation, after-ripening, add stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 5%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 48 DEG C, fermentation 40h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 6 DEG C of refrigerators and preserves 18h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
Embodiment 3
A kind of composite probiotics ferment lichee sour milk beverage, is obtained by following composition:
Concentrated Lychee juice 15 parts, lactobacillus bulgaricus 2 parts, streptococcus thermophilus 3 parts, Bifidobacterium 2 parts, xanthans 13 parts, pectin 7 parts, CMC13 part, white granulated sugar 14 parts, citric acid 9 parts;
A preparation method for composite probiotics ferment lichee sour milk beverage, preparation technology is:
Inoculate in concentrated Lychee juice, fermentation, after-ripening, add stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 3%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 43 DEG C, fermentation 30h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 4 DEG C of refrigerators and preserves 14h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
Embodiment 4
A kind of composite probiotics ferment lichee sour milk beverage, is obtained by following composition:
Concentrated Lychee juice 20 parts, lactobacillus bulgaricus 2 parts, streptococcus thermophilus 2 parts, Bifidobacterium 2 parts, xanthans 14 parts, pectin 8 parts, CMC14 part, white granulated sugar 18 parts, citric acid 13 parts;
A preparation method for composite probiotics ferment lichee sour milk beverage, preparation technology is:
Inoculate in concentrated Lychee juice, fermentation, after-ripening, add stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 4%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 40 DEG C, fermentation 25h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 5 DEG C of refrigerators and preserves 12h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
Embodiment 5
A kind of composite probiotics ferment lichee sour milk beverage, is obtained by following composition:
Concentrated Lychee juice 20 parts, lactobacillus bulgaricus 3 parts, streptococcus thermophilus 4 parts, Bifidobacterium 3 parts, xanthans 15 parts, pectin 10 parts, CMC15 part, white granulated sugar 20 parts, citric acid 15 parts;
A preparation method for composite probiotics ferment lichee sour milk beverage, preparation technology is:
Inoculate in concentrated Lychee juice, fermentation, after-ripening, add stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 3.5%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 46 DEG C, fermentation 35h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 5 DEG C of refrigerators and preserves 16h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.

Claims (9)

1. a preparation method for composite probiotics ferment lichee sour milk beverage, is characterized in that: comprise the steps:
(1) inoculate: inoculate in concentrated Lychee juice, inoculation is aseptically carried out, and inoculum concentration is 2-5%, inoculates three kinds of beneficial bacteriums;
(2) ferment: lichee concentrate fermentation step (1) inoculated, the condition of fermentation is: at temperature 38-48 DEG C, fermentation 20-40h;
(3) after-ripening: after fermentation ends, cooling liquid is also placed in 3-6 DEG C of refrigerator and preserves 10-18h, carries out after fermentation after-ripening;
(4) composite: the liquid of after-ripening is added appropriate stabilizing agent, liquid glucose, water, acid, broken curdled milk, mix, homogeneous, sterile filling.
2. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1, is characterized in that: described beneficial bacterium is lactobacillus bulgaricus, streptococcus thermophilus and Bifidobacterium.
3. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1 and 2, is characterized in that: the addition of described lactobacillus bulgaricus is 1-3 part, the addition of streptococcus thermophilus is 2-4 part, the addition of Bifidobacterium is 1-3 part.
4. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1, is characterized in that: the addition of described concentrated Lychee juice is 10-20 part.
5. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1, is characterized in that: described stabilizing agent is xanthans, pectin and CMC.
6. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1 or 5, is characterized in that: described stabilizing agent is the addition that the addition of xanthans is 10-15 part, pectin addition is 5-10 part and CMC is 10-15 part.
7. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1, is characterized in that: described liquid glucose is white granulated sugar solution, and the consumption of white granulated sugar is 10-20 part.
8. the preparation method of a kind of composite probiotics ferment lichee sour milk beverage according to claim 1, is characterized in that: described acid is citric acid, and the addition of citric acid is 5-15 part.
9. a composite probiotics ferment lichee sour milk beverage, is characterized in that: the composite probiotics ferment lichee sour milk beverage that described beverage is prepared for method described in claim 1-8 any one.
CN201410752388.0A 2014-12-11 2014-12-11 Preparation method of litchi lactic acid bacteria drink by employing complex probiotic fermentation Pending CN104544390A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105475605A (en) * 2015-12-28 2016-04-13 福建省农业科学院农业生物资源研究所 Preparation method for litchi and lactic acid bacteria fermented ice cream
CN106509115A (en) * 2016-11-16 2017-03-22 北京银河雪生物科技开发有限公司 Double-fermentation lactic acid bacteria beverage, and preparation method thereof
CN109259026A (en) * 2018-11-30 2019-01-25 浙江海洋大学 A kind of compound probiotic beverage and preparation method thereof
CN112293619A (en) * 2019-07-31 2021-02-02 谢葳 Multi-strain microbial compound beverage
CN114606084A (en) * 2022-04-14 2022-06-10 广东石油化工学院 Preparation method of litchi wine

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD182Y2 (en) * 2003-07-25 2008-07-31 Anatoly Anatolyevich Kutyev Reservoir with oxygenated beverage
CN101720955A (en) * 2009-11-10 2010-06-09 上海乐全食品有限公司 Method and system for fermenting and preparing beverage by adopting concentrated fruit and vegetable juice
CN102309041A (en) * 2010-12-04 2012-01-11 赵敏 Fresh lychee probiotic beverage and preparation method thereof
CN103815483A (en) * 2014-02-17 2014-05-28 广东省农业科学院蚕业与农产品加工研究所 Viable type lactobacillus fermented litchi juice beverage and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MD182Y2 (en) * 2003-07-25 2008-07-31 Anatoly Anatolyevich Kutyev Reservoir with oxygenated beverage
CN101720955A (en) * 2009-11-10 2010-06-09 上海乐全食品有限公司 Method and system for fermenting and preparing beverage by adopting concentrated fruit and vegetable juice
CN102309041A (en) * 2010-12-04 2012-01-11 赵敏 Fresh lychee probiotic beverage and preparation method thereof
CN103815483A (en) * 2014-02-17 2014-05-28 广东省农业科学院蚕业与农产品加工研究所 Viable type lactobacillus fermented litchi juice beverage and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105475605A (en) * 2015-12-28 2016-04-13 福建省农业科学院农业生物资源研究所 Preparation method for litchi and lactic acid bacteria fermented ice cream
CN106509115A (en) * 2016-11-16 2017-03-22 北京银河雪生物科技开发有限公司 Double-fermentation lactic acid bacteria beverage, and preparation method thereof
CN109259026A (en) * 2018-11-30 2019-01-25 浙江海洋大学 A kind of compound probiotic beverage and preparation method thereof
CN112293619A (en) * 2019-07-31 2021-02-02 谢葳 Multi-strain microbial compound beverage
CN114606084A (en) * 2022-04-14 2022-06-10 广东石油化工学院 Preparation method of litchi wine

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Application publication date: 20150429