CN107782890A - A kind of preparation method of type Ⅳ collagen protein detection kit - Google Patents
A kind of preparation method of type Ⅳ collagen protein detection kit Download PDFInfo
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- CN107782890A CN107782890A CN201710915573.0A CN201710915573A CN107782890A CN 107782890 A CN107782890 A CN 107782890A CN 201710915573 A CN201710915573 A CN 201710915573A CN 107782890 A CN107782890 A CN 107782890A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
The invention discloses a kind of preparation method of type Ⅳ collagen protein detection kit, comprise the following steps：(1) reagent R1 constituent contents are pressed, prepare MOPSO buffer solutions, pH are adjusted, as R1 buffer solutions；By NaCl, NaN3, Arabic gum, Tween 80, PEG 2000 be dissolved in R1 buffer solutions, obtain reagent R1；(2) reagent R2 constituent contents are pressed, prepare MOPSO buffer solutions, pH are adjusted, as R2 buffer solutions；By NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions, obtain R2 dispersion liquids；Prepare the coated type Ⅳ collagen protein polyclone antibody of latex；The coated type Ⅳ collagen protein polyclone antibody of latex is dissolved with R2 dispersion liquids, obtains reagent R2.Advantages of the present invention is：Simple to operate, quick, cost is low, pollution-free；Kit sensitivity is good, the degree of accuracy is high, the range of linearity is wide；Stabilization of kit is good, high specificity；It is adapted to full-automatic testing.
Description
Technical field
The present invention relates to technical field of medical examination, more particularly to a kind of preparation side of type Ⅳ collagen protein detection kit
Method.
Background technology
Collagen is a kind of fibrous glycoprotein, and it is the α-peptide chain network structure formed by triple helix body.Send out at present
Existing collagen is present in different tissues up to as many as 10 kinds.Type Ⅳ collagen albumen is the important component for forming basilar memebrane.Base in normal hepatocytes
Counterdie is primarily present in blood vessel, lymphatic vessel, around bile duct, lacks at sinus hepaticus wall.Increase in hepatopathy with inflammatory development, fibr tissue
Life jump, has a large amount of collagen depositions in fibr tissue generating process, and various collagens increased, but wherein it is mostly important just
It is the increase for the type Ⅳ collagen albumen for forming basilar memebrane.It is now recognized that the measure of type Ⅳ collagen albumen, which can be used as, checks liver fiber
The modern age index of change.
At present, the method for detecting type Ⅳ collagen albumen is mainly radioimmunoassay method, but radioimmunoassay method
Concrete operations it is relatively cumbersome, and certain radiocontamination be present, put into the problems such as more.
Therefore, it is badly in need of a kind of preparation of type Ⅳ collagen protein detection kit simple to operate, pollution-free, cost is low at present
Method.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide it is a kind of it is simple to operate, pollution-free, cost is low
The preparation method of type Ⅳ collagen protein detection kit.
The present invention is achieved by the following technical solutions：A kind of preparation method of type Ⅳ collagen protein detection kit,
Comprise the following steps：
(1) according to following component content reagent preparation R1：
Reagent R1：
1. according to mentioned reagent R1 constituent content, MOPSO buffer solutions are prepared, and adjusted with watery hydrochloric acid or sodium hydroxide
PH, as R1 buffer solutions；
2. according to mentioned reagent R1 constituent content, by NaCl, NaN3, Arabic gum, Tween-80, PEG-2000 be dissolved in
In R1 buffer solutions, stir, treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) according to following component content reagent preparation R2：
Reagent R2：
1. according to mentioned reagent R2 constituent content, MOPSO buffer solutions are prepared, and adjusted with watery hydrochloric acid or sodium hydroxide
PH, as R2 buffer solutions；
2. according to mentioned reagent R2 constituent content, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions, stirring is equal
It is even, produce R2 dispersion liquids；
3. prepare the coated type Ⅳ collagen protein polyclone antibody of latex：
A. the latex microsphere that particle diameter is 80nm and 120nm is taken in MES buffer solutions, is added EDAC solution and is mixed after 37 DEG C
It is incubated in environment and mixes 1h, supernatant is removed in centrifugation；Add NHS solution to recover to original volume, mix and be incubated in 37 DEG C of environment
1h is mixed, centrifugation removes supernatant, must mix microballoon emulsion；
B. the polyethylene p-chloromethyl styrene copolymer of said components content is added in microballoon emulsion is mixed, at 37 DEG C
At a temperature of react 1-3h, be copolymerized the latex of 80nm and 120nm particle diameters；Centrifugation, surplus materials is scattered in MES buffer solutions
In, 2-4 times repeatedly, most, to original volume, type Ⅳ collagen protein polyclone antibody is added, in 37 after recovery in MES buffer solutions
3-4h is reacted at DEG C, centrifuges, sediment is scattered in the PBS of original volume, 2-4 times repeatedly, finally by sediment point
Dissipate in the NHS buffer solutions of original volume, add BSA；
C.2-8 DEG C seal 45-50h up for safekeeping, obtain the coated type Ⅳ collagen protein polyclone antibody of finally required latex；
4. dissolve the obtained coated type Ⅳ collagen protein polyclone antibody of latex with R2 dispersion liquids, ultrasonic disperse, most
Obtained reagent R2 eventually；Wherein, ultimate density of the polyethylene p-chloromethyl styrene copolymer in reagent R2 is 9%-11%.
One of preferred embodiment as the present invention, in the step (1), the pH of R1 buffer solutions is 6.5.
One of preferred embodiment as the present invention, in the step (2), the pH of R2 buffer solutions is 6.5.
One of preferred embodiment as the present invention, the step for preparing the coated type Ⅳ collagen protein polyclone antibody of latex
In rapid a, after taking latex microsphere that particle diameter is 80nm and 120nm in MES buffer solutions, the content of 80nm latex microspheres is 20g/L,
The content of 120nm latex microspheres is 40g/L.
One of preferred embodiment as the present invention, the step for preparing the coated type Ⅳ collagen protein polyclone antibody of latex
In rapid a, the MES buffer solutions used is 100mM MES buffer solutions.
One of preferred embodiment as the present invention, the step for preparing the coated type Ⅳ collagen protein polyclone antibody of latex
In rapid a, the EDAC solution that uses for 50g/L EDAC solution.
One of preferred embodiment as the present invention, the step for preparing the coated type Ⅳ collagen protein polyclone antibody of latex
In rapid a, the NHS solution that uses for 50g/L NHS solution.
One of preferred embodiment as the present invention, the step for preparing the coated type Ⅳ collagen protein polyclone antibody of latex
In rapid b, the BSA of addition is 30g/L BSA.
The present invention compared with prior art the advantages of be：
(1) kit prepared using this method has higher detection sensitivity and a degree of accuracy, and use it is simple to operate,
Quickly, from detecting out that result only needs 10 minutes；
(2) antigen antibody complex formed using kit prepared by this method with sample, good stability, specific
There are certain absorbance, high specificity under wavelength；In addition, the latex microsphere that this method takes different-grain diameter is used in mixed way, greatly
The big sensitivity and linear measurement range for improving kit；
(3) kit prepared using this method need not be equipped with special large-scale instrument and equipment and use, and cost is low, without dirt
Dye；
(4) kit prepared using this method can be used on automatic clinical chemistry analyzer, be adapted to full-automatic testing, can be big
The development and popularization of scale.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of preparation method of type Ⅳ collagen protein detection kit of the present embodiment, comprises the following steps：
(1) according to following component content reagent preparation R1：
Reagent R1：
1. according to mentioned reagent R1 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide
To 6.5, as R1 buffer solutions；
2. according to mentioned reagent R1 constituent content, by NaCl, NaN3, Arabic gum, Tween-80, PEG-2000 be dissolved in
In R1 buffer solutions, stir, treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) according to following component content reagent preparation R2：
Reagent R2：
1. according to mentioned reagent R2 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide
To 6.5, as R2 buffer solutions；
2. according to mentioned reagent R2 constituent content, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions, stirring is equal
It is even, produce R2 dispersion liquids；
3. prepare the coated type Ⅳ collagen protein polyclone antibody of latex：
A. the latex microsphere that particle diameter is 80nm (20g/L) and 120nm (40g/L) is taken to be added in 100mM MES buffer solutions
50g/L EDAC solution, mix after being incubated mixing 1h in 37 DEG C of environment, supernatant is removed in centrifugation；Add 50g/L NHS solution
Recover to original volume, mix and mixing 1h is incubated in 37 DEG C of environment, centrifugation removes supernatant, must mix microballoon emulsion；
B. the polyethylene p-chloromethyl styrene copolymer of said components content is added in microballoon emulsion is mixed, at 37 DEG C
At a temperature of react 1h, be copolymerized the latex of 80nm and 120nm particle diameters；Centrifugation, surplus materials is scattered in MES buffer solutions,
2 times repeatedly, most after recovery in MES buffer solutions to original volume, type Ⅳ collagen protein polyclone antibody is added, it is anti-at 37 DEG C
3h is answered, centrifuges, sediment is scattered in the PBS of original volume, 2 times repeatedly, sediment is finally scattered in original volume
NHS buffer solutions in, add BSA 30g/L；
C.2 DEG C seal 45h up for safekeeping, obtain the coated type Ⅳ collagen protein polyclone antibody of finally required latex；
4. dissolve the obtained coated type Ⅳ collagen protein polyclone antibody of latex with R2 dispersion liquids, ultrasonic disperse, most
Obtained reagent R2 eventually；Wherein, ultimate density of the polyethylene p-chloromethyl styrene copolymer in reagent R2 is 9%.
Embodiment 2
A kind of preparation method of type Ⅳ collagen protein detection kit of the present embodiment, comprises the following steps：
(1) according to following component content reagent preparation R1：
Reagent R1：
1. according to mentioned reagent R1 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide
To 6.5, as R1 buffer solutions；
2. according to mentioned reagent R1 constituent content, by NaCl, NaN3, Arabic gum, Tween-80, PEG-2000 be dissolved in
In R1 buffer solutions, stir, treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) according to following component content reagent preparation R2：
Reagent R2：
1. according to mentioned reagent R2 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide
To 6.5, as R2 buffer solutions；
2. according to mentioned reagent R2 constituent content, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions, stirring is equal
It is even, produce R2 dispersion liquids；
3. prepare the coated type Ⅳ collagen protein polyclone antibody of latex：
A. the latex microsphere that particle diameter is 80nm (20g/L) and 120nm (40g/L) is taken to be added in 100mM MES buffer solutions
50g/L EDAC solution, mix after being incubated mixing 1h in 37 DEG C of environment, supernatant is removed in centrifugation；Add 50g/L NHS solution
Recover to original volume, mix and mixing 1h is incubated in 37 DEG C of environment, centrifugation removes supernatant, must mix microballoon emulsion；
B. the polyethylene p-chloromethyl styrene copolymer of said components content is added in microballoon emulsion is mixed, at 37 DEG C
At a temperature of react 3h, be copolymerized the latex of 80nm and 120nm particle diameters；Centrifugation, surplus materials is scattered in MES buffer solutions,
4 times repeatedly, most after recovery in MES buffer solutions to original volume, type Ⅳ collagen protein polyclone antibody is added, it is anti-at 37 DEG C
4h is answered, centrifuges, sediment is scattered in the PBS of original volume, 4 times repeatedly, sediment is finally scattered in original volume
NHS buffer solutions in, add BSA 30g/L；
C.8 DEG C seal 50h up for safekeeping, obtain the coated type Ⅳ collagen protein polyclone antibody of finally required latex；
4. dissolve the obtained coated type Ⅳ collagen protein polyclone antibody of latex with R2 dispersion liquids, ultrasonic disperse, most
Obtained reagent R2 eventually；Wherein, ultimate density of the polyethylene p-chloromethyl styrene copolymer in reagent R2 is 11%.
Embodiment 3
A kind of preparation method of type Ⅳ collagen protein detection kit of the present embodiment, comprises the following steps：
(1) according to following component content reagent preparation R1：
Reagent R1：
1. according to mentioned reagent R1 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide
To 6.5, as R1 buffer solutions；
2. according to mentioned reagent R1 constituent content, by NaCl, NaN3, Arabic gum, Tween-80, PEG-2000 be dissolved in
In R1 buffer solutions, stir, treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) according to following component content reagent preparation R2：
Reagent R2：
1. according to mentioned reagent R2 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide
To 6.5, as R2 buffer solutions；
2. according to mentioned reagent R2 constituent content, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions, stirring is equal
It is even, produce R2 dispersion liquids；
3. prepare the coated type Ⅳ collagen protein polyclone antibody of latex：
A. the latex microsphere that particle diameter is 80nm (20g/L) and 120nm (40g/L) is taken to be added in 100mM MES buffer solutions
50g/L EDAC solution, mix after being incubated mixing 1h in 37 DEG C of environment, supernatant is removed in centrifugation；Add 50g/L NHS solution
Recover to original volume, mix and mixing 1h is incubated in 37 DEG C of environment, centrifugation removes supernatant, must mix microballoon emulsion；
B. the polyethylene p-chloromethyl styrene copolymer of said components content is added in microballoon emulsion is mixed, at 37 DEG C
At a temperature of react 2h, be copolymerized the latex of 80nm and 120nm particle diameters；Centrifugation, surplus materials is scattered in MES buffer solutions,
3 times repeatedly, most after recovery in MES buffer solutions to original volume, type Ⅳ collagen protein polyclone antibody is added, it is anti-at 37 DEG C
3.5h is answered, centrifuges, sediment is scattered in the PBS of original volume, 3 times repeatedly, sediment is finally scattered in substance
In long-pending NHS buffer solutions, BSA 30g/L are added；
C.4 DEG C seal 48h up for safekeeping, obtain the coated type Ⅳ collagen protein polyclone antibody of finally required latex；
4. dissolve the obtained coated type Ⅳ collagen protein polyclone antibody of latex with R2 dispersion liquids, ultrasonic disperse, most
Obtained reagent R2 eventually；Wherein, ultimate density of the polyethylene p-chloromethyl styrene copolymer in reagent R2 is 10%.
Embodiment 4
The user of the type Ⅳ collagen protein detection kit being prepared using above-described embodiment method of the present embodiment
Method, comprise the following steps：
(1) 5uL testing samples are mixed with 200uL reagents R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined at wavelength 660nm with automatic clinical chemistry analyzer；
(3) mixed again with 50uL reagents R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined at wavelength 660nm with automatic clinical chemistry analyzer；
(5) according to absorbance change value Δ A=A2-A1, the concentration of type Ⅳ collagen albumen in sample is calculated.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (8)
1. a kind of preparation method of type Ⅳ collagen protein detection kit, it is characterised in that comprise the following steps：
(1) according to following component content reagent preparation R1：
Reagent R1：
1. according to mentioned reagent R1 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide, are made
For R1 buffer solutions；
2. according to mentioned reagent R1 constituent content, by NaCl, NaN3, Arabic gum, Tween-80, PEG-2000 be dissolved in R1 and delay
In fliud flushing, stir, treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) according to following component content reagent preparation R2：
Reagent R2：
1. according to mentioned reagent R2 constituent content, MOPSO buffer solutions are prepared, and pH is adjusted with watery hydrochloric acid or sodium hydroxide, are made
For R2 buffer solutions；
2. according to mentioned reagent R2 constituent content, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions, stir, i.e.,
Obtain R2 dispersion liquids；
3. prepare the coated type Ⅳ collagen protein polyclone antibody of latex：
A. the latex microsphere that particle diameter is 80nm and 120nm is taken in MES buffer solutions, is added EDAC solution and is mixed after 37 DEG C of environment
Middle be incubated mixes 1h, and supernatant is removed in centrifugation；Add NHS solution to recover to original volume, mix and mixing is incubated in 37 DEG C of environment
1h, centrifugation remove supernatant, must mix microballoon emulsion；
B. the polyethylene p-chloromethyl styrene copolymer of said components content is added in microballoon emulsion is mixed, in 37 DEG C of temperature
Lower reaction 1-3h, it is copolymerized the latex of 80nm and 120nm particle diameters；Centrifugation, surplus materials is scattered in MES buffer solutions, instead
It is multiple 2-4 times, most after recovery in MES buffer solutions to original volume, type Ⅳ collagen protein polyclone antibody is added, it is anti-at 37 DEG C
3-4h is answered, centrifuges, sediment is scattered in the PBS of original volume, 2-4 times repeatedly, sediment is finally scattered in original
In the NHS buffer solutions of volume, BSA is added；
C.2-8 DEG C seal 45-50h up for safekeeping, obtain the coated type Ⅳ collagen protein polyclone antibody of finally required latex；
4. dissolve the obtained coated type Ⅳ collagen protein polyclone antibody of latex, ultrasonic disperse, final system with R2 dispersion liquids
Obtain reagent R2；Wherein, ultimate density of the polyethylene p-chloromethyl styrene copolymer in reagent R2 is 9%-11%.
2. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the step
(1) in, the pH of R1 buffer solutions is 6.5.
3. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the step
(2) in, the pH of R2 buffer solutions is 6.5.
4. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the preparation
In the step a of the coated type Ⅳ collagen protein polyclone antibody of latex, the latex microsphere that particle diameter is 80nm and 120nm is taken in MES
After in buffer solution, the content of 80nm latex microspheres is 20g/L, and the content of 120nm latex microspheres is 40g/L.
5. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the preparation
In the step a of the coated type Ⅳ collagen protein polyclone antibody of latex, the MES buffer solutions used is 100mM MES buffer solutions.
6. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the preparation
In the step a of the coated type Ⅳ collagen protein polyclone antibody of latex, the EDAC solution that uses for 50g/L EDAC solution.
7. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the preparation
In the step a of the coated type Ⅳ collagen protein polyclone antibody of latex, the NHS solution that uses for 50g/L NHS solution.
8. the preparation method of type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that the preparation
In the step b of the coated type Ⅳ collagen protein polyclone antibody of latex, the BSA of addition is 30g/L BSA.
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| CN201710915573.0A CN107782890A (en) | 2017-09-30 | 2017-09-30 | A kind of preparation method of type Ⅳ collagen protein detection kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108659127A (en) * | 2018-04-03 | 2018-10-16 | 东莞光极生物科技有限公司 | L10D9 antibody and its application |
| CN110531088A (en) * | 2019-09-26 | 2019-12-03 | 阿里生物技术泰州有限公司 | A kind of detection method of IV collagen type detection kit |
| CN111122875A (en) * | 2020-01-02 | 2020-05-08 | 四川纳海川生物科技有限公司 | IV-type collagen reagent detection kit and preparation method thereof |
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| JP2000146973A (en) * | 1998-11-17 | 2000-05-26 | Sekisui Chem Co Ltd | Method and reagent for immunoassay of iv type collagen |
| CN103134926A (en) * | 2013-02-27 | 2013-06-05 | 上海交通大学 | Magnetic microsphere carrier and its making method |
| CN106053839A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining haptoglobin and preparation method thereof |
| CN106932588A (en) * | 2015-12-30 | 2017-07-07 | 上海复星长征医学科学有限公司 | Detection α1Kit of-microglobulin and preparation method thereof |
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2017
- 2017-09-30 CN CN201710915573.0A patent/CN107782890A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000146973A (en) * | 1998-11-17 | 2000-05-26 | Sekisui Chem Co Ltd | Method and reagent for immunoassay of iv type collagen |
| CN103134926A (en) * | 2013-02-27 | 2013-06-05 | 上海交通大学 | Magnetic microsphere carrier and its making method |
| CN106932588A (en) * | 2015-12-30 | 2017-07-07 | 上海复星长征医学科学有限公司 | Detection α1Kit of-microglobulin and preparation method thereof |
| CN106053839A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining haptoglobin and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108659127A (en) * | 2018-04-03 | 2018-10-16 | 东莞光极生物科技有限公司 | L10D9 antibody and its application |
| CN110531088A (en) * | 2019-09-26 | 2019-12-03 | 阿里生物技术泰州有限公司 | A kind of detection method of IV collagen type detection kit |
| CN111122875A (en) * | 2020-01-02 | 2020-05-08 | 四川纳海川生物科技有限公司 | IV-type collagen reagent detection kit and preparation method thereof |
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