CN107703307A - A kind of type Ⅳ collagen protein detection kit and its application method - Google Patents
A kind of type Ⅳ collagen protein detection kit and its application method Download PDFInfo
- Publication number
- CN107703307A CN107703307A CN201710914665.7A CN201710914665A CN107703307A CN 107703307 A CN107703307 A CN 107703307A CN 201710914665 A CN201710914665 A CN 201710914665A CN 107703307 A CN107703307 A CN 107703307A
- Authority
- CN
- China
- Prior art keywords
- reagent
- type
- collagen protein
- detection kit
- protein detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
The invention discloses a kind of type Ⅳ collagen protein detection kit, including reagent R1：MOPSO buffer solutions, NaCl, NaN3, Arabic gum, Tween 80, PEG 2000, solvent is purified water；Reagent R2：MOPSO buffer solutions, NaCl, NaN3, BSA, glycerine, the coated type Ⅳ collagen protein polyclone antibody of latex, solvent is purified water；The raw material of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen protein polyclone antibody and polyethylene p-chloromethyl styrene copolymer.The invention also discloses a kind of application method of type Ⅳ collagen protein detection kit.The advantage of the invention is that：(1) kit has compared with high detection sensitivity and the degree of accuracy, simple to operate, quick；(2) antigen antibody complex formed, good stability, high specificity；(3) cost is low, pollution-free；(4) it is used for automatic clinical chemistry analyzer.
Description
Technical field
The present invention relates to technical field of medical examination, more particularly to a kind of type Ⅳ collagen protein detection kit and its use
Method.
Background technology
Collagen is a kind of fibrous glycoprotein, and it is the α-peptide chain network structure formed by triple helix body.Send out at present
Existing collagen is present in different tissues up to as many as 10 kinds.Type Ⅳ collagen albumen is the important component for forming basilar memebrane.Base in normal hepatocytes
Counterdie is primarily present in blood vessel, lymphatic vessel, around bile duct, lacks at sinus hepaticus wall.Increase in hepatopathy with inflammatory development, fibr tissue
Life jump, has a large amount of collagen depositions in fibr tissue generating process, and various collagens increased, but wherein it is mostly important just
It is the increase for the type Ⅳ collagen albumen for forming basilar memebrane.It is now recognized that the measure of type Ⅳ collagen albumen, which can be used as, checks liver fiber
The modern age index of change.
At present, the method for detecting type Ⅳ collagen albumen is mainly radioimmunoassay method, but radioimmunoassay method
Concrete operations it is relatively cumbersome, and certain radiocontamination be present, put into the problems such as more.
Therefore, it is badly in need of a kind of type Ⅳ collagen protein detection kit simple to operate, pollution-free, cost is low at present.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide it is a kind of it is simple to operate, pollution-free, cost is low
Type Ⅳ collagen protein detection kit and its application method.
The present invention is achieved by the following technical solutions：A kind of type Ⅳ collagen protein detection kit, including it is only each other
Vertical reagent R1 and reagent R2 biliquid components, including composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 9%-11%.
One of preferred embodiment as the present invention, including reagent R1 independent of each other and reagent R2 biliquid components, including
Composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10%.
One of preferred embodiment as the present invention, including reagent R1 independent of each other and reagent R2 biliquid components, including
Composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10.5%.
One of preferred embodiment as the present invention, the pH of the MOPSO buffer solutions of the reagent R1 is 6.5.
One of preferred embodiment as the present invention, the pH of the MOPSO buffer solutions of the reagent R2 is 6.5.
A kind of application method of type Ⅳ collagen protein detection kit, comprises the following steps：
(1) testing sample is mixed with reagent R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined with automatic clinical chemistry analyzer；
(3) mixed again with reagent R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined with automatic clinical chemistry analyzer；
(5) concentration of type Ⅳ collagen albumen in sample is calculated according to absorbance change value.
One of preferred embodiment as the present invention, the reagent R1 of the step (1) and the reagent R2 of step (3) volume ratio
For 4:1.
One of preferred embodiment as the present invention, testing sample and the reagent R1 and reagent R2 compositions of the step (1)
Volume ratio between reagent solution is 1:50.
One of preferred embodiment as the present invention, in the step (2) and step (4), extinction is determined at wavelength 660nm
Spend A1 and A2.
One of preferred embodiment as the present invention, in the step (5), absorbance change value is Δ A, i.e., after A2 subtracts A1
Value.
The present invention compared with prior art the advantages of be：
(1) kit of the present invention has higher detection sensitivity and the degree of accuracy, and use is simple to operate, quick, from inspection
Measuring out result only needs 10 minutes；
(2) antigen antibody complex that the present invention is formed, good stability, there is certain absorbance at a particular wavelength,
High specificity；
(3) present invention need not be equipped with special large-scale instrument and equipment, and cost is low, pollution-free；
(4) it can be used on automatic clinical chemistry analyzer, be adapted to full-automatic testing, can carry out and promote on a large scale.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of type Ⅳ collagen protein detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 9%.
Embodiment 2
A kind of type Ⅳ collagen protein detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 11%.
Embodiment 3
A kind of type Ⅳ collagen protein detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10%.
Embodiment 4
A kind of type Ⅳ collagen protein detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Its solvent is purified water；
Reagent R2：
Its solvent is purified water；
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen egg
White polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10.5%.
Embodiment 5
The preparation method of type Ⅳ collagen protein detection kit in a kind of above-described embodiment of the present embodiment, including following step
Suddenly：
(1) reagent preparation R1：
1. according to the constituent content of reagent R1 in above-described embodiment, MOPSO buffer solutions are prepared, and with watery hydrochloric acid or hydroxide
Sodium adjusts pH to 6.5, as R1 buffer solutions；
2. according to the constituent content of reagent R1 in above-described embodiment, by NaCl, NaN3, Arabic gum, Tween-80, PEG-
2000 are dissolved in R1 buffer solutions, stir, and treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) reagent preparation R2：
1. according to the constituent content of reagent R2 in above-described embodiment, MOPSO buffer solutions are prepared, and with watery hydrochloric acid or hydroxide
Sodium adjusts pH to 6.5, as R2 buffer solutions；
2. according to the constituent content of reagent R2 in above-described embodiment, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions
In, stir, produce R2 dispersion liquids；
3. prepare the coated type Ⅳ collagen protein polyclone antibody of latex：
A. the latex microsphere that particle diameter is 80nm (20g/L) and 120nm (40g/L) is taken to be added in 100mM MES buffer solutions
50g/L EDAC solution, mix after being incubated mixing 1h in 37 DEG C of environment, supernatant is removed in centrifugation；Add 50g/L NHS solution
Recover to original volume, mix and mixing 1h is incubated in 37 DEG C of environment, centrifugation removes supernatant, must mix microballoon emulsion；
B. the polyethylene p-chloromethyl styrene copolymer of said components content is added in microballoon emulsion is mixed, at 37 DEG C
At a temperature of react 2h, be copolymerized the latex of 80nm and 120nm particle diameters；Centrifugation, surplus materials is scattered in MES buffer solutions,
3 times repeatedly, most after recovery in MES buffer solutions to original volume, type Ⅳ collagen protein polyclone antibody is added, it is anti-at 37 DEG C
3.5h is answered, centrifuges, sediment is scattered in the PBS of original volume, 3 times repeatedly, sediment is finally scattered in substance
In long-pending NHS buffer solutions, BSA 30g/L are added；
C.4 DEG C seal 48h up for safekeeping, obtain the coated type Ⅳ collagen protein polyclone antibody of finally required latex；
4. dissolve the obtained coated type Ⅳ collagen protein polyclone antibody of latex with R2 dispersion liquids, ultrasonic disperse, most
Obtained reagent R2 eventually.
Embodiment 6
The application method of type Ⅳ collagen protein detection kit in a kind of above-described embodiment of the present embodiment, including following step
Suddenly：
(1) 5uL testing samples are mixed with 200uL reagents R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined at wavelength 660nm with automatic clinical chemistry analyzer；
(3) mixed again with 50uL reagents R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined at wavelength 660nm with automatic clinical chemistry analyzer；
(5) according to absorbance change value Δ A=A2-A1, the concentration of type Ⅳ collagen albumen in sample is calculated.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of type Ⅳ collagen protein detection kit, it is characterised in that including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen albumen are more
Clonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is in overall examination
Content in agent R2 is 9%-11%.
2. type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that including reagent independent of each other
R1 and reagent R2 biliquid components, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen albumen are more
Clonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is in overall examination
Content in agent R2 is 10%.
3. type Ⅳ collagen protein detection kit according to claim 1, it is characterised in that including reagent independent of each other
R1 and reagent R2 biliquid components, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated type Ⅳ collagen protein polyclone antibody of latex is：Latex microsphere, type Ⅳ collagen albumen are more
Clonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is in overall examination
Content in agent R2 is 10.5%.
4. according to the type Ⅳ collagen protein detection kit described in claim any one of 1-3, it is characterised in that the reagent R1
MOPSO buffer solutions pH be 6.5.
5. according to the type Ⅳ collagen protein detection kit described in claim any one of 1-3, it is characterised in that the reagent R2
MOPSO buffer solutions pH be 6.5.
6. a kind of method of type Ⅳ collagen protein detection kit using as described in claim any one of 1-3, its feature exist
In comprising the following steps：
(1) testing sample is mixed with reagent R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined with automatic clinical chemistry analyzer；
(3) mixed again with reagent R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined with automatic clinical chemistry analyzer；
(5) concentration of type Ⅳ collagen albumen in sample is calculated according to absorbance change value.
7. the application method of type Ⅳ collagen protein detection kit according to claim 6, it is characterised in that the step
(1) reagent R1 and the reagent R2 of step (3) volume ratio is 4:1.
8. the application method of type Ⅳ collagen protein detection kit according to claim 6, it is characterised in that the step
(1) volume ratio between the reagent solution that testing sample and reagent R1 and reagent R2 is formed is 1:50.
9. the application method of type Ⅳ collagen protein detection kit according to claim 6, it is characterised in that the step
(2) and in step (4), absorbance A 1 and A2 are determined at wavelength 660nm.
10. the application method of type Ⅳ collagen protein detection kit according to claim 6, it is characterised in that the step
Suddenly in (5), absorbance change value is Δ A, i.e. A2 subtracts the value after A1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710914665.7A CN107703307B (en) | 2017-09-30 | 2017-09-30 | IV-type collagen detection kit and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710914665.7A CN107703307B (en) | 2017-09-30 | 2017-09-30 | IV-type collagen detection kit and use method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107703307A true CN107703307A (en) | 2018-02-16 |
| CN107703307B CN107703307B (en) | 2020-04-14 |
Family
ID=61183235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710914665.7A Active CN107703307B (en) | 2017-09-30 | 2017-09-30 | IV-type collagen detection kit and use method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107703307B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110531088A (en) * | 2019-09-26 | 2019-12-03 | 阿里生物技术泰州有限公司 | A kind of detection method of IV collagen type detection kit |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000146973A (en) * | 1998-11-17 | 2000-05-26 | Sekisui Chem Co Ltd | Method and reagent for immunoassay of iv type collagen |
| CN1475805A (en) * | 2002-08-15 | 2004-02-18 | 陕西西大北美基因股份有限公司 | Magnetic fluorescence microsphere and its preparation method and method of proceeding biomolecule detection using said magnetic fluorescence microsphere |
| CN103134926A (en) * | 2013-02-27 | 2013-06-05 | 上海交通大学 | Magnetic microsphere carrier and its making method |
| CN106053839A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining haptoglobin and preparation method thereof |
| JP2017083440A (en) * | 2015-10-23 | 2017-05-18 | 株式会社Ｌｓｉメディエンス | Immunoassay reagent, assay method, and method of expanding measurement range |
-
2017
- 2017-09-30 CN CN201710914665.7A patent/CN107703307B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000146973A (en) * | 1998-11-17 | 2000-05-26 | Sekisui Chem Co Ltd | Method and reagent for immunoassay of iv type collagen |
| CN1475805A (en) * | 2002-08-15 | 2004-02-18 | 陕西西大北美基因股份有限公司 | Magnetic fluorescence microsphere and its preparation method and method of proceeding biomolecule detection using said magnetic fluorescence microsphere |
| CN103134926A (en) * | 2013-02-27 | 2013-06-05 | 上海交通大学 | Magnetic microsphere carrier and its making method |
| JP2017083440A (en) * | 2015-10-23 | 2017-05-18 | 株式会社Ｌｓｉメディエンス | Immunoassay reagent, assay method, and method of expanding measurement range |
| CN106053839A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining haptoglobin and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110531088A (en) * | 2019-09-26 | 2019-12-03 | 阿里生物技术泰州有限公司 | A kind of detection method of IV collagen type detection kit |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107703307B (en) | 2020-04-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104714007B (en) | Immunochemical detection of single target entities | |
| CN109738626A (en) | NGAL latex immunoturbidimetry detection kit and preparation method thereof | |
| CN107894509B (en) | A method of improving latex immunoturbidimetry antigen excess and the range of linearity | |
| CN107782890A (en) | A kind of preparation method of type Ⅳ collagen protein detection kit | |
| CN101196518A (en) | Hepatitis virus type C immune body chemiluminescence method diagnostic reagent kit and its producing method | |
| CN106872718A (en) | A kind of microdose urine protein detection kit and preparation method thereof | |
| CN102944679A (en) | Kit for performing retinol binding protein detection by using latex turbidimetry | |
| CN108037283B (en) | A kind of antibody diluent and its preparation method and application for enzyme linked immunosorbent detection | |
| Sanderson et al. | The use of protein tyrosine phosphatase 1B and insulin receptor immunostains to differentiate nonalcoholic from alcoholic steatohepatitis in liver biopsy specimens | |
| CN107703307A (en) | A kind of type Ⅳ collagen protein detection kit and its application method | |
| CN107741494A (en) | A kind of preparation method of CER detection kit | |
| CN107255723A (en) | A kind of kit detected for myoglobins | |
| CN106053840A (en) | Kit for measuring alpha1-microglobulin and preparation method of kit | |
| CN105181969A (en) | Kit for content detection of neutrophil gelatinase-associated lipocalin | |
| CN108152518A (en) | A kind of kit for measuring Serum CG and its preparation application method | |
| CN108627652A (en) | It is a kind of to detect based on simple grain diameter and simultaneously the kit of RBP ELISA in serum and urine specimen | |
| CN102937650B (en) | Preparation method of membrane strip of kit for detecting autoantibody spectrum related to autoimmune liver disease (AILD) and kit composed of same | |
| CN111320696A (en) | MMP-3 antibody compound based on streptavidin latex and kit thereof | |
| CN107677840A (en) | A kind of parathyroid hormone detection kit and its application method | |
| CN107741504A (en) | A kind of preparation method of parathyroid hormone detection kit | |
| CN107741402A (en) | A kind of CER detection kit and its application method | |
| CN107703290A (en) | A kind of preparation method of blood vessel endothelial factor detection kit | |
| CN107121326B (en) | The fast acid of red blood cell release diffuses method | |
| CN107727847A (en) | A kind of blood vessel endothelial factor detection kit and its application method | |
| CN106405067B (en) | A kind of detection method of the drug target residence time based on full cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 236000 no.659 Lanzhou Road, Baohe District, Hefei City, Anhui Province Patentee after: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. Address before: 236000 floor 1-4, building 18, phase I, Liandong u Valley, southeast corner of the intersection of Huaxing Avenue and Jilin Road, Baohe Economic Development Zone, Hefei City, Anhui Province Patentee before: ANHUI IPROCOM BIOTECHNOLOGY Co.,Ltd. |