CN107782838A - A kind of clearing lung and eliminating phlegm stops the method for quality control for medicine of coughing - Google Patents
A kind of clearing lung and eliminating phlegm stops the method for quality control for medicine of coughing Download PDFInfo
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Abstract
The invention discloses the method for quality control that a kind of clearing lung and eliminating phlegm stops medicine of coughing, belong to pharmaceutical field.A kind of clearing lung and eliminating phlegm stops the quality determining method for medicine of coughing, and step is：1st, need testing solution is prepared：Candidate drug is taken as need testing solution；2nd, control medicinal material solution is prepared：Fritillaria thunbergii medicinal material is taken, control medicinal material solution is made；3rd, reference substance solution is prepared；4th, thin-layer chromatography：Each 4 μ l of above two solution are drawn respectively, puts on same silica gel g thin-layer plate, is deployed with solvent respectively, are taken out, are dried, spray with dilute bismuth potassium iodide test solution；5th, result judgement.The present invention is in lung clearing cough suppressing phlegm transforming medicinal ball on the basis of primary standard, establish the thin-layer identification method of fritillaria thunbergii, filter out revised quality standard in the method inclusive criteria that favorable reproducibility, specificity are strong, spot development is clear, result easily judges, improve the quality control of medicine, make the qualitative detection of medicine more accurate, quality is more prone to control.
Description
Technical field
The present invention relates to the method for quality control that a kind of clearing lung and eliminating phlegm stops medicine of coughing, belong to pharmaceutical field.
Background technology
The lung clearing cough suppressing phlegm transforming medicinal ball prescription is using radix scutellariae, root of Chinese trichosanthes as main ingredient, the clear dry heat-phlegm syndrome of purging the lung of pathogenic fire intestines and internal organs of the body internal organs；With the root of purple-flowered peucedanum,
Abundant expectoration due to lung-heat cough is controlled supplemented by balloonflower root, fritillaria thunbergii；To be relievingd asthma with semen armeniacae amarae, the root bark of white mulberry, stemona root cough relieving, the tuber of dwarf lilyturf, the wind-weed nourish lung yin,
Rheum officinale is equipped with root of Chinese trichosanthes and rushes down big internal organs heat, with Exocarpium Citri Grandis, Fructus Aurantii regulating qi-flowing for eliminating phlegm, and prevents rheum officinale, radix scutellariae from rushing down clearly too, for assistant
Medicine；Radix glycyrrhizae coordinating the drug actions of a prescription.The ten four traditional Chinese medicines collaboration of full side works, and is exclusively used in treating the yellow phlegm of cough caused by abundant expectoration due to lung-heat, chest diaphragm is not
Freely, sound of having sore throat is mute, is hard and dry.For bronchitis, the pneumonia person that occurs above-mentioned symptom.
Lung clearing cough suppressing phlegm transforming medicinal ball prescription：Radix scutellariae 96g, root of Chinese trichosanthes 48g, fritillaria thunbergii 24g, Fructus Aurantii (going flesh bran to fry) 24g, balloonflower root
It is 24g, the root bark of white mulberry (honey is processed) 24g, prepared RADIX ET RHIZOMA RHEI 12g, wind-weed 12g, tuber of dwarf lilyturf 12g, Exocarpium Citri Grandis 12g, semen armeniacae amarae (peeling fry) 12g, preceding
Hu 12g, tuber of stemona 12g, radix glycyrrhizae 3g.
Lung clearing cough suppressing phlegm transforming medicinal ball preparation method：14 tastes by more than, are ground into fine powder, sieve, and mix, and with water pill, dry, i.e.,
.
Major function：Clearing lung-heat, resolving sputum, only cough.For the yellow phlegm of cough caused by lung heat phlegm, chest diaphragm is not smooth, and laryngalgia sound is mute, greatly
Just dry.
Existing standard record in《Drug Standard of Ministry of Public Health of the Peoples Republic of China》The 7th (Chinese traditional patent formulation of in 1993 version
Preparation) page 172 (WS3-B-1433-93).Appearance character, microscopical characters and rhubarb medicinal material are only defined in its quality standard
Thin-layer identification method, can not fundamentally qualitative, quantitative control product inherent quality.Through retrieval, find no Guan Qingjin and stop
The patent document for phlegm-resolving pill detection method of coughing.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of clear gold that is simple, being capable of accurate qualitative detection drug ingedient to stop
Cough the method for quality control of phlegm-resolving pill.
To achieve the above object, the present invention uses following technical scheme：
A kind of clearing lung and eliminating phlegm stops the quality determining method for medicine of coughing, and step is：
(1) need testing solution is prepared：Candidate drug 3~10g is taken, it is finely ground, add 20~40ml of water to make dissolving, water-bath to 70~
90 DEG C, 0.5~2% hydrochloric acid is adjusted to pH value as 1~3, centrifugation, takes supernatant, with 3~10% sodium hydroxides be adjusted to pH value for 9~
12, with extracted by ether 2~3 times, 10~30ml, merges extract solution, is evaporated every time, and residue adds 2~5ml of methanol to make dissolving, as
Need testing solution；
(2) reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made
Contain 1~3mg solution per 1ml, as reference substance solution；
(3) control medicinal material solution is prepared：Fritillaria thunbergii medicinal material 1~5g is taken, it is finely ground, add 10~50ml of water to make dissolving, water-bath is extremely
70~90 DEG C, it is 1~3 that 0.5~2% hydrochloric acid, which is adjusted to pH value, centrifugation, takes supernatant, being adjusted to pH value with 3~10% sodium hydroxides is
8~12, with extracted by ether 2~3 times, 10~30ml, merges extract solution, is evaporated every time, and residue adds 2~5ml of methanol to make dissolving, makees
For need testing solution；
(4) thin-layer chromatography：Each 3~5 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with
Ethyl acetate-methanol-conc. aq. ammonia is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；
(5) result judgement.
Preferably, the step (1) prepares need testing solution：Candidate drug 5g is taken, it is finely ground, add water 30ml to make dissolving, water
To 80 DEG C, it is 2 that 1% hydrochloric acid, which is adjusted to pH value, centrifugation, takes supernatant for bath, and it is 11 to be adjusted to pH value with 5% sodium hydroxide, is carried with ether
Take 3 times, each 20ml, merge extract solution, be evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
Preferably, the step (2) prepares reference substance solution：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively
Add chloroform that solution of every 1ml containing 2mg is made, as reference substance solution；
Preferably, the step (3) prepares control medicinal material solution：Fritillaria thunbergii medicinal material 2g is taken, it is finely ground, add water 30ml to make molten
Solution, to 80 DEG C, it is 2 that 1% hydrochloric acid, which is adjusted to pH value, centrifugation, takes supernatant for water-bath, and it is 11 to be adjusted to pH value with 5% sodium hydroxide, uses second
Ether extracts 3 times, each 20ml, merges extract solution, is evaporated, residue adds methanol 3ml to make dissolving, as need testing solution；
Preferably, step (4) thin-layer chromatography：Each 4 μ l of above two solution are drawn respectively, are put respectively in same silica gel
On G lamellaes, using ethyl acetate-methanol-conc. aq. ammonia as solvent, deploy, take out, dry, spray with dilute bismuth potassium iodide test solution；Institute
State ethyl acetate in solvent：Methanol：The volume ratio of concentrated ammonia liquor is 15~20：3~1：3~1.
Preferably, ethyl acetate in the solvent：Methanol：The volume ratio of concentrated ammonia liquor is 18：2：1.
Step (5) result judgement refers to：Contrast the chromatogram of test sample, reference substance and control medicinal material；Wherein test sample
In chromatogram, on position corresponding with reference substance and control medicinal material chromatogram, the spot person for showing same color is qualified.
It is lung clearing cough suppressing phlegm transforming medicinal ball that clearing lung and eliminating phlegm of the present invention, which stops medicine of coughing,.
The prescription of the lung clearing cough suppressing phlegm transforming medicinal ball is：Radix scutellariae 96g, root of Chinese trichosanthes 48g, fritillaria thunbergii 24g, Fructus Aurantii (going flesh bran to fry)
24g, balloonflower root 24g, the root bark of white mulberry (honey is processed) 24g, prepared RADIX ET RHIZOMA RHEI 12g, wind-weed 12g, tuber of dwarf lilyturf 12g, Exocarpium Citri Grandis 12g, semen armeniacae amarae (peeling
Fry) 12g, root of purple-flowered peucedanum 12g, tuber of stemona 12g, radix glycyrrhizae 3g.
The preparation method of the lung clearing cough suppressing phlegm transforming medicinal ball is：14 tastes by more than, are ground into fine powder, sieve, and mix, general with water
Ball, dry, produce.
A kind of clearing lung and eliminating phlegm stops the quality determining method for medicine of coughing：
1st, need testing solution is prepared：Candidate drug 5g is taken, it is finely ground, add water 30ml to make dissolving, water-bath is to 80 DEG C, 1% hydrochloric acid
PH value is adjusted to as 2, centrifugation, takes supernatant, pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, each 20ml, is closed
And extract solution, it is evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
2nd, reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made often
Solution of the 1ml containing 2mg, as reference substance solution；
3rd, control medicinal material solution is prepared：Fritillaria thunbergii medicinal material 2g is taken, it is finely ground, add water 30ml to make dissolving, water-bath is to 80 DEG C, 1%
Hydrochloric acid is adjusted to pH value as 2, centrifugation, takes supernatant, and pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, every time
20ml, merge extract solution, be evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
3rd, thin-layer chromatography：Each 4 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with acetic acid
Ethyl ester-methanol-conc. aq. ammonia (﹕ 1 of 18 ﹕ 2) is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution.
4th, result judgement：Contrast the chromatogram of test sample, reference substance and control medicinal material；Wherein in test sample chromatogram, with it is right
According on product and the corresponding position of control medicinal material chromatogram, the spot person of aobvious same color is qualified.
Step 4 thin-layer chromatography, according to thin-layered chromatography (《Chinese Pharmacopoeia》Four annex general rules 0502 of version in 2015)
Experiment is carried out.
It is an advantage of the invention that：The present invention on the basis of primary standard, establishes the thin of fritillaria thunbergii in lung clearing cough suppressing phlegm transforming medicinal ball
Layer discrimination method, filters out and is repaiied in the method inclusive criteria that favorable reproducibility, specificity are strong, spot development is clear, result easily judges
Quality standard after ordering, the quality control of medicine is improved, make the qualitative detection of medicine more accurate, quality is more prone to control
System.
The present invention is elaborated with reference to the accompanying drawings and detailed description, not to the limitation of invention.It is all according to
This area equivalent substitution that the disclosure of invention is carried out, belongs to protection scope of the present invention.
Brief description of the drawings
Fig. 1 is the measurement result figure of embodiment 1
Embodiment
Embodiment 1
First, material
Test sample：Lung clearing cough suppressing phlegm transforming medicinal ball, Lerentang Pharmaceutical Factory, Zhongxin Pharmaceutical Group Co., Ltd., Tianj, lot number：
160801、160802、160803。
Reference substance：Peimine reference substance, National Institute for Food and Drugs Control, lot number 110750-201311,
Peininine reference substance, National Institute for Food and Drugs Control, lot number 110751-201110
Control medicinal material：Fritillaria thunbergii medicinal material, National Institute for Food and Drugs Control, lot number 120972-201405.
It is prepared by negative controls：The technique and prescription announced according to pharmacopeia prepare the drug sample without fritillaria thunbergii.
2nd, detect
1st, need testing solution is prepared：Candidate drug 5g is taken, it is finely ground, add water 30ml to make dissolving, water-bath is to 80 DEG C, with 1% salt
Acid is adjusted to pH value as 2, centrifuges 10 minutes, takes supernatant, and pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, often
Secondary 20ml, merge extract solution, be evaporated, residue adds methanol 3ml to make dissolving, produces；
2nd, reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made often
Solution of the 1ml containing 2mg, is produced；
3rd, control medicinal material solution is prepared：Fritillaria thunbergii medicinal material 2g is taken, it is finely ground, add water 30ml to make dissolving, water-bath is to 80 DEG C, 1%
Hydrochloric acid is adjusted to pH value as 2, centrifugation, takes supernatant, and pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, every time
20ml, merge extract solution, be evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
4th, negative controls solution is prepared：Negative controls 5g is taken, is carried out by need testing solution same method is prepared.
5th, thin-layer chromatography：Each 4 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with acetic acid
Ethyl ester-methanol-conc. aq. ammonia (volume ratio 18：2：1) it is solvent, deploys, takes out, dry, spray with dilute bismuth potassium iodide test solution.
6th, result judgement：Contrast test sample, reference substance and control medicinal material, the chromatogram of negative controls；Wherein test sample color
In spectrum, on position corresponding with reference substance and control medicinal material chromatogram, the spot person for showing same color is qualified.
3rd, result
Three batches of test samples differentiate as stated above.As a result Fig. 1 is seen, 1-7 is followed successively by from left to right：Peimine reference substance,
Peininine reference substance, fritillaria thunbergii medicinal material, lung clearing cough suppressing phlegm transforming medicinal ball 160801, lung clearing cough suppressing phlegm transforming medicinal ball 160802, lung clearing cough suppressing
Phlegm-resolving pill 160803, negative controls.
It is being in phase with fritillaria Su Jia ﹑ Peininines reference substances and fritillaria thunbergii medicinal material chromatogram relevant position in test sample chromatogram
With colored speckles spot, and feminine gender is noiseless.
Embodiment 2
First, material
Test sample：Lung clearing cough suppressing phlegm transforming medicinal ball, Lerentang Pharmaceutical Factory, Zhongxin Pharmaceutical Group Co., Ltd., Tianj, lot number：
160801、160802、160803。
Reference substance：Peimine reference substance, National Institute for Food and Drugs Control, lot number 110750-201311,
Peininine reference substance, National Institute for Food and Drugs Control, lot number 110751-201110
Control medicinal material：Fritillaria thunbergii medicinal material, National Institute for Food and Drugs Control, lot number 120972-201405.
2nd, detect
1st, need testing solution is prepared：Candidate drug 5g is taken, it is finely ground, add water 30ml to make dissolving, water-bath is to 80 DEG C, with 1% salt
Acid is adjusted to pH value as 2, centrifuges 10 minutes, takes supernatant, and pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, often
Secondary 20ml, merge extract solution, be evaporated, residue adds methanol 3ml to make dissolving, produces；
2nd, reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made often
Solution of the 1ml containing 2mg, is produced；
3rd, control medicinal material solution is prepared：Fritillaria thunbergii medicinal material 2g is taken, it is finely ground, add water 30ml to make dissolving, water-bath is to 80 DEG C, 1%
Hydrochloric acid is adjusted to pH value as 2, centrifugation, takes supernatant, and pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, every time
20ml, merge extract solution, be evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
4th, thin-layer chromatography：Each 4 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with acetic acid
Ethyl ester-methanol-conc. aq. ammonia (volume ratio 18：2：1) it is solvent, deploys, takes out, dry, spray with dilute bismuth potassium iodide test solution.
5th, result judgement：Contrast test sample, reference substance and control medicinal material, the chromatogram of negative controls；Wherein test sample color
In spectrum, on position corresponding with reference substance and control medicinal material chromatogram, the spot person for showing same color is qualified.
3rd, result
Three batches of test samples differentiate as stated above.In test sample chromatogram, with fritillaria Su Jia ﹑ Peininines reference substances and Zhejiang
Fritillaria medicinal material chromatogram relevant position is in same color spot spot.
Embodiment 3
First, material
Test sample：Lung clearing cough suppressing phlegm transforming medicinal ball, Lerentang Pharmaceutical Factory, Zhongxin Pharmaceutical Group Co., Ltd., Tianj, lot number：
160801、160802、160803。
Reference substance：Peimine reference substance, National Institute for Food and Drugs Control, lot number 110750-201311
Peininine reference substance, National Institute for Food and Drugs Control, lot number 110751-201110
Control medicinal material：Fritillaria thunbergii medicinal material, National Institute for Food and Drugs Control, lot number 120972-201405
2nd, detect
1st, need testing solution is prepared：Candidate drug 3g is taken, it is finely ground, add water 20ml to make dissolving, water-bath is to 70 DEG C, 0.5% salt
Acid is adjusted to pH value as 3, centrifugation, takes supernatant, and pH value is adjusted to as 9 with 3% sodium hydroxide, with extracted by ether 2 times, each 10ml,
Merge extract solution, be evaporated, residue adds methanol 2ml to make dissolving, as need testing solution.
2nd, reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made often
Solution of the 1ml containing 1mg, as reference substance solution；
3rd, control medicinal material solution is prepared：Take fritillaria thunbergii medicinal material 1g, it is finely ground, add water 10ml to make dissolving, water-bath to 70 DEG C,
0.5% hydrochloric acid is adjusted to pH value as 1, centrifugation, takes supernatant, and pH value is adjusted to as 12 with 3% sodium hydroxide, with extracted by ether 2 times, often
Secondary 10ml, merge extract solution, be evaporated, residue adds methanol 2ml to make dissolving, as need testing solution；
4th, thin-layer chromatography：Each 3 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with acetic acid
Ethyl ester-methanol-conc. aq. ammonia is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；Ethyl acetate in solvent：
Methanol：The volume ratio of concentrated ammonia liquor is 20：3：3.
5th, result judgement：Contrast the chromatogram of test sample, reference substance and control medicinal material；Wherein in test sample chromatogram, with it is right
According on product and the corresponding position of control medicinal material chromatogram, the spot person of aobvious same color is qualified.
Embodiment 4
First, material
Test sample：Lung clearing cough suppressing phlegm transforming medicinal ball, Lerentang Pharmaceutical Factory, Zhongxin Pharmaceutical Group Co., Ltd., Tianj, lot number：
160801、160802、160803。
Reference substance：Peimine reference substance, National Institute for Food and Drugs Control, lot number 110750-201311,
Peininine reference substance, National Institute for Food and Drugs Control, lot number 110751-201110
Control medicinal material：Fritillaria thunbergii medicinal material, National Institute for Food and Drugs Control, lot number 120972-201405.
2nd, detect
1st, need testing solution is prepared：Candidate drug 10g is taken, it is finely ground, add water 40ml to make dissolving, water-bath is to 90 DEG C, 2% hydrochloric acid
PH value is adjusted to as 1, centrifugation, takes supernatant, pH value is adjusted to as 9 with 10% sodium hydroxide, with extracted by ether 3 times, each 30ml, is closed
And extract solution, it is evaporated, residue adds methanol 5ml to make dissolving, as need testing solution.
2nd, reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made often
Solution of the 1ml containing 3mg, as reference substance solution；
3rd, control medicinal material solution is prepared：Fritillaria thunbergii medicinal material 5g is taken, it is finely ground, add water 50ml to make dissolving, water-bath is to 90 DEG C, 2%
Hydrochloric acid is adjusted to pH value as 1, centrifugation, takes supernatant, and pH value is adjusted to as 8 with 10% sodium hydroxide, with extracted by ether 3 times, every time
30ml, merge extract solution, be evaporated, residue adds methanol 5ml to make dissolving, as need testing solution；
4th, thin-layer chromatography：Each 5 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with acetic acid
Ethyl ester-methanol-conc. aq. ammonia is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；Ethyl acetate in solvent：
Methanol：The volume ratio of concentrated ammonia liquor is 15：1：1.
5th, result judgement：Contrast the chromatogram of test sample, reference substance and control medicinal material；Wherein in test sample chromatogram, with it is right
According on product and the corresponding position of control medicinal material chromatogram, the spot person of aobvious same color is qualified.
Claims (10)
1. a kind of quality determining method of clearing lung and eliminating phlegm cough suppressing medicine, it is characterised in that step is：
(1) need testing solution is prepared：Candidate drug 3~10g is taken, it is finely ground, add 20~40ml of water to make dissolving, water-bath to 70~90
DEG C, it is 1~3 that 0.5~2% hydrochloric acid, which is adjusted to pH value, centrifugation, takes supernatant, and it is 9~12 to be adjusted to pH value with 3~10% sodium hydroxides,
With extracted by ether 2~3 times, 10~30ml, merges extract solution, is evaporated every time, and residue adds 2~5ml of methanol to make dissolving, as trying
Product solution；
(2) reference substance solution is prepared：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus every 1ml is made in chloroform
Solution containing 1~3mg, as reference substance solution；
(3) control medicinal material solution is prepared：Fritillaria thunbergii medicinal material 1~5g is taken, it is finely ground, add 10~50ml of water to make dissolving, water-bath to 70~
90 DEG C, 0.5~2% hydrochloric acid is adjusted to pH value as 1~3, centrifugation, takes supernatant, with 3~10% sodium hydroxides be adjusted to pH value for 8~
12, with extracted by ether 2~3 times, 10~30ml, merges extract solution, is evaporated every time, and residue adds 2~5ml of methanol to make dissolving, as
Need testing solution；
(4) thin-layer chromatography：Each 3~5 μ l of above two solution are drawn respectively, are put respectively on same silica gel g thin-layer plate, with acetic acid
Ethyl ester-methanol-conc. aq. ammonia is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；
(5) result judgement.
2. a kind of clearing lung and eliminating phlegm according to claim 1 stops the quality determining method for medicine of coughing, it is characterised in that：The step
Suddenly (1) prepares need testing solution：Candidate drug 5g is taken, it is finely ground, add water 30ml to make dissolving, to 80 DEG C, 1% hydrochloric acid is adjusted to PH for water-bath
It is worth for 2, centrifugation, takes supernatant, pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, each 20ml, merges extraction
Liquid, it is evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
3. a kind of clearing lung and eliminating phlegm according to claim 1 stops the quality determining method for medicine of coughing, it is characterised in that：The step
Suddenly (2) prepare reference substance solution：Peimine Dui Zhao Pin ﹑ Peininine reference substances are taken, respectively plus chloroform is made every 1ml and contained
2mg solution, as reference substance solution.
4. a kind of clearing lung and eliminating phlegm according to claim 1 stops the quality determining method for medicine of coughing, it is characterised in that：The step
Suddenly (3) prepare control medicinal material solution：Fritillaria thunbergii medicinal material 2g is taken, it is finely ground, add water 30ml to make dissolving, to 80 DEG C, 1% hydrochloric acid is adjusted for water-bath
It is 2 to pH value, centrifugation, takes supernatant, pH value is adjusted to as 11 with 5% sodium hydroxide, with extracted by ether 3 times, each 20ml, closes
And extract solution, it is evaporated, residue adds methanol 3ml to make dissolving, as need testing solution.
5. a kind of clearing lung and eliminating phlegm according to claim 1 stops the quality determining method for medicine of coughing, it is characterised in that：The step
Suddenly (4) thin-layer chromatography：Draw each 4 μ l of above two solution respectively, put respectively on same silica gel g thin-layer plate, with ethyl acetate-
Methanol-conc. aq. ammonia is solvent, is deployed, and takes out, dries, spray with dilute bismuth potassium iodide test solution；Ethyl acetate in the solvent：First
Alcohol：The volume ratio of concentrated ammonia liquor is 15~20：3~1：3~1.
6. a kind of clearing lung and eliminating phlegm according to claim 5 stops the quality determining method for medicine of coughing, it is characterised in that：The exhibition
Open ethyl acetate in agent：Methanol：The volume ratio of concentrated ammonia liquor is 18：2：1.
7. a kind of clearing lung and eliminating phlegm according to claim 1 stops the quality determining method for medicine of coughing, it is characterised in that：The step
Suddenly (5) result judgement refers to：Contrast the chromatogram of test sample, reference substance and control medicinal material；Wherein in test sample chromatogram, with compareing
On product and the corresponding position of control medicinal material chromatogram, the spot person for showing same color is qualified.
8. a kind of clearing lung and eliminating phlegm according to any one of claim 1 to 7 stops the quality determining method for medicine of coughing, it is special
Sign is：It is lung clearing cough suppressing phlegm transforming medicinal ball that the clearing lung and eliminating phlegm, which stops medicine of coughing,.
9. a kind of clearing lung and eliminating phlegm according to claim 8 stops the quality determining method for medicine of coughing, it is characterised in that：It is described clear
The prescription of golden Zhisou-Huatan tablet is：Radix scutellariae 96g, root of Chinese trichosanthes 48g, fritillaria thunbergii 24g, Fructus Aurantii (going flesh bran to fry) 24g, balloonflower root 24g, mulberry
White skin (honey is processed) 24g, prepared RADIX ET RHIZOMA RHEI 12g, wind-weed 12g, tuber of dwarf lilyturf 12g, Exocarpium Citri Grandis 12g, semen armeniacae amarae (peeling is fried) 12g, root of purple-flowered peucedanum 12g,
Tuber of stemona 12g, radix glycyrrhizae 3g.
10. a kind of clearing lung and eliminating phlegm according to claim 9 stops the quality determining method for medicine of coughing, it is characterised in that：It is described
The preparation method of lung clearing cough suppressing phlegm transforming medicinal ball is：14 tastes by more than, are ground into fine powder, sieve, and mix, and with water pill, dry, produce.
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Non-Patent Citations (3)
| Title |
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| 中华人民共和国卫生部药典委员会: "《卫生部颁药品标准（中药成方制剂第七册）》", 31 December 1993 * |
| 中国药典委员会: "《中国药典1995版一部》", 31 December 1995 * |
| 刘志辉等: "润喉开音片有效成分的定性定量研究 ", 《中成药》 * |
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