CN107779479A - Produce ethanol saccharomyces cerevisiae composition and its method for producing ethanol - Google Patents
Produce ethanol saccharomyces cerevisiae composition and its method for producing ethanol Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The invention provides a kind of method for the composition and its fermentative production of ethanol for strengthening new saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae), said composition composition includes starchy material, nitrogen source, phosphorus source, enzyme preparation, inorganic salts, glucoamylase and new dry ferment.Said composition formula is applied to wine-making technology, saccharomyces neoformans can be promoted to make full use of starchy material, strengthens alcohol metabolism, reduces the usage amount of glucoamylase, reduces alcohol production cost, has great economic benefit and social benefit.The new saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae) is preserved in China typical culture collection center, and deposit number is CCTCC No.M2014657.
Description
Technical field
The invention belongs to bioengineering field, is related to alcoholic fermentation, and in particular to one kind strengthens new Ethanol in Saccharomyces cerevisiae
The saccharomyces cerevisiae composition of metabolism and its technique for producing ethanol.
Background technology
At present, China is more using base starting materials such as corn, cassava, sugar grasses, by crushing, liquefying, being saccharified, fermenting and steaming
Evaporate the production for realizing alcohol.The drawbacks of existing production technology process is more, high energy consumption, low labor productivity gradually show；In addition it is near
Carry out increasing price of raw materials, production cost increase, the continual reductions of relevant policies subsidy, the profit margin of alcohol industry is increasingly
It is few.
High density fermentation (HCDF) in alcohol industry, which has, to be shortened fermentation period, reduces equipment investment, idetified separation, carries
The advantages such as high comprehensive production efficiency.But too high concentration of glucose directly affects yeast growth breeding, fermenting speed, fermentation
Intensity and fermentation time.The bottleneck of the unbalanced always high cell density fermentation of nutrition supply.Concentration of glucose is too high or too low
The growth and breeding of unfavorable yeast, it is alcohol to be also unfavorable in yeast cells zymase by the fermentation of sugared part.
Saccharification of the fermentation by saccharomyces cerevisiae process after Starch Hydrolysis is combined by simultaneous saccharification and fermentation technology (SSF), is not required to
Hydrolysis reactor is additionally added, equipment requirement can be reduced, simplify operating process, what is more important glucose is once hydrolysis
It is ethanol by yeast sorption enhanced, can significantly alleviates the negative effect that high glucose concentration is brought.However, optimum hydrolysis temperature is
45-50 DEG C, optimum fermentation temp is 28-35 DEG C.Usual synchronous fermentation temperature at 35-38 DEG C, it is this handle the activity that makes enzyme and
Fermentation efficiency can not all reach maximum.
The content of the invention
In summary, for conventional art, alcohol industry faces subject matter：The situation about falling sharply of profit margin
Under, high gravity fermentation process concentration of glucose suppresses alcoholic fermentation；Synchronous fermentation process fermentation temperature and Starch Hydrolysis temperature
Incoordination causes the dosage of carbohydrase big.
It is an object of the invention to provide a kind of wine-making technology formula and its producer for strengthening Ethanol in Saccharomyces cerevisiae metabolism
Method, the defects of overcoming prior art.
Specifically, in view of the shortcomings of the prior art, the invention provides following technical scheme：
First technical scheme is a kind of fermentative production of ethanol saccharomyces cerevisiae composition, and it contains nitrogen source, phosphorus source thing
Matter, enzyme preparation, inorganic salts, glucoamylase and saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae), the wine
Brewer yeast is preserved in China typical culture collection center, and deposit number is CCTCC No.M2014657, and the enzyme preparation is not wrapped
Include glucoamylase.
Saccharomyces cerevisiae 1522 used has been described in the patent of Application No. 2015100830825 in the present invention.
Preferably, foregoing fermentative production of ethanol saccharomyces cerevisiae composition, it contains fermentative production of ethanol matrix original
Material, nitrogen source, phosphorus source material, enzyme preparation, inorganic salts, glucoamylase and the (Saccharomyces of saccharomyces cerevisiae 1522
Cerevisiae), the saccharomyces cerevisiae is preserved in China typical culture collection center, deposit number CCTCC
No.M2014657, the enzyme preparation do not include glucoamylase, and the base starting material is that starchy material and/or molasses matter are former
Material.
Preferably, foregoing saccharomyces cerevisiae composition, starchy material therein are selected from corn, tapioca starch, sorghum or paddy
It is one or more of in thing.
Preferably, foregoing saccharomyces cerevisiae composition, wherein relative to fermentative production of ethanol base starting material gross dry weight amount,
Following substances content is respectively：0.1~1g/100g of nitrogen source, preferably 0.15-1g/100g, 0.01~1g/100g of phosphorus source, enzyme preparation
10~50u/g, 0.001~1g/100g of inorganic salts, 5~50u/g of glucoamylase and saccharomyces cerevisiae 1522
(Saccharomyces cerevisiae) 0.05~0.5g/100g, preferably 0.1-0.5g/100g, wherein the saccharomyces cerevisiae
Weight be yeast dry weight.
Preferably, foregoing saccharomyces cerevisiae composition, wherein, enzyme preparation contains phytase 2-5u/g and selected from acidic protein
At least one of enzyme, cellulase, hemicellulase and zytase enzyme 8-45u/g, wherein beyond above-mentioned phytase
Other enzyme contents are：Acid protease 0-25u/g, preferably 1-25u/g, preferably cellulase 0-10u/g, 1-10u/g, hemicellulose
Plain enzyme 0-25u/g, preferably 1-25u/g and zytase 0-10u/g, preferably 1-10u/g.
Preferably, foregoing saccharomyces cerevisiae composition, wherein, relative to fermentative production of ethanol raw material gross dry weight amount, under
Stating content of material is respectively：Nitrogen source 0.15-0.25g/100g, 0.01~1g/100g of phosphorus source, 30~45u/g of enzyme preparation, inorganic salts
0.001~1g/100g, 30~45u/g of glucoamylase and saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae)
0.1~0.3g/100g.
Preferably, foregoing saccharomyces cerevisiae composition, it is described relative to fermentative production of ethanol base starting material gross dry weight amount
Enzyme preparation contains following components：Acid protease 5-25u/g, phytase 2-5u/g, cellulase 2-10u/g, hemicellulase
20-25u/g and zytase 3-10u/g.
Preferably, foregoing saccharomyces cerevisiae composition, wherein, nitrogen source is selected from urea, ammonium sulfate, ammonium di-hydrogen phosphate, phosphoric acid
One or two or more kinds in one hydrogen ammonia；Phosphorus source is selected from potassium dihydrogen phosphate and/or dipotassium hydrogen phosphate；Enzyme preparation is selected from acidic protein
Two kinds in enzyme, phytase, cellulase, hemicellulase or zytase or more than three kinds；Inorganic salts are selected from ammonium sulfate, sulphur
One or two or more kinds in sour zinc, magnesium sulfate, potassium chloride, sodium chloride, ferrous sulfate, copper sulphate, calcium chloride or manganese sulfate.
Second of technical scheme is a kind of method that ethanol is produced using fermentation by saccharomyces cerevisiae, using foregoing wine brewing ferment
Female feedstock composition carries out fermenting and producing, comprises the following steps：Water is added into foregoing saccharomyces cerevisiae feedstock composition to obtain
Mixture, fermented.
Preferably, the foregoing method that ethanol is produced using fermentation by saccharomyces cerevisiae, wherein, material water weight ratio is 1：(2-5).
Preferably, the foregoing method that ethanol is produced using fermentation by saccharomyces cerevisiae, wherein, after adding water, adjust described mixed
Compound pH value to 4.5-5.3 carry out fermentative production of ethanol.
Preferably, the foregoing method that ethanol is produced using fermentation by saccharomyces cerevisiae, wherein, after adding water, adjust described mixed
The temperature of compound is 32-36 DEG C, preferably 32-33 DEG C, carries out fermentative production of ethanol.
Preferably, the foregoing method that ethanol is produced using fermentation by saccharomyces cerevisiae, wherein, after adding water, to the mixing
Thing stirring carries out fermentative production of ethanol, and the speed of agitator is 150-180rmp.
Second of technical scheme is that foregoing saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae) is in fermenting and producing
Application in ethanol, the saccharomyces cerevisiae are preserved in China typical culture collection center, deposit number CCTCC
No.M2014657。
Compared with prior art, the beneficial effects of the present invention are：It can promote new saccharomyces cerevisiae secretion glucose to form sediment
Powder enzyme, strengthen yeast-alcohol metabolic function；Glucoamylase enzyme dosage is reduced, reduces production cost, improves the effect of alcohol production
Benefit；Improve and perfect diastatic fermentation synchronous process, promotes the development of high density fermentation.
Bacterial strain preservation information of the present invention
Strain Saccharomyces cerevisiae 1522, category saccharomyces cerevisiae (Saccharomyces cerevisiae), the saccharomyces cerevisiae are protected
It is hidden in China typical culture collection center (CCTCC), preservation address：No. 299 Wuhan of Wuhan City, Hubei Province Wuchang District Bayi Road
(the first attached primary school of Wuhan University opposite), Wuhan University's collection, postcode in institution of higher education：430072；Preservation date：2014
On December 23, in；Deposit number is CCTCC No.M2014657.
Embodiment
The invention provides a kind of method for strengthening new Ethanol in Saccharomyces cerevisiae metabolism, this method comprises the following steps：
Base starting material removal of impurities is crushed, liquefaction processing, be cooled to 30 DEG C~40 DEG C.In terms of base starting material dry weight, add respectively
Enter 0~1g/100g of nitrogen source, 0~1g/100g of phosphorus source, 0~50u/g of enzyme preparation, 0~1g/100g of inorganic salts, fully mix.With base
Matter raw material dry weight meter, 0~50u/g glucoamylases are added, are fully mixed.0~0.5g/100g of new dry ferment dosage, fills
Divide and mix.Distilled water is added to material-water ratio is set, adjusts appropriate pH, temperature, rotating speed carry out alcoholic fermentation.68h measure of fermenting exists
Wine part, residual reduced sugar under above-mentioned condition, residual total reducing sugar.
Wherein, in the above method of the present invention, from base starting material be domestic alcohol industry production corn, tapioca starch,
Sorghum, cereal etc.；
In the above method of the present invention, nitrogen source mainly includes urea, ammonium sulfate, ammonium di-hydrogen phosphate, the hydrogen ammonia of phosphoric acid one.At this
In the invention above method, phosphorus source mainly includes potassium dihydrogen phosphate, dipotassium hydrogen phosphate.
In the above method of the present invention, enzyme preparation mainly includes acid protease, phytase, cellulase, complex enzyme, wood
Dextranase.
In the above method of the present invention, inorganic salts mainly include ammonium sulfate, zinc sulfate, magnesium sulfate, potassium chloride, sodium chloride,
Ferrous sulfate, copper sulphate, calcium chloride, manganese sulfate.
In a kind of embodiment of the present invention, there is provided the technique for strengthening new Ethanol in Saccharomyces cerevisiae metabolism is matched somebody with somebody
Side, formula composition include base starting material, nitrogen source, phosphorus source, enzyme preparation, inorganic salts, glucoamylase and dry ferment.With matrix
Raw material dry weight meter, each component content are as follows：0.1~1g/100g of nitrogen source, preferably 0.15~1g/100g, 0.01~1g/ of phosphorus source
100g, 10~50u/g of enzyme preparation, 0.001~1g/100g of inorganic salts, 20~50u/g of glucoamylase and the present invention's is above-mentioned
New dry ferment CCTCC No.M2014657 0.05~0.5g/100g, preferably 0.1~0.5g/100g, the enzyme preparation are not wrapped
Glucoamylase is included, the base starting material is starchy material.
In the preferred embodiment of the present invention, the formula includes for the method and step of wine-making technology：By matrix
Impurity removing crushes, liquefaction processing, is cooled to 30 DEG C~40 DEG C；Then, each component of formulation content is added, is mixed, is added water, adjusts
Save pH, temperature and rotating speed and carry out alcoholic fermentation.
Below with reference to embodiment to strengthening the wine-making technology formula and its use that new Ethanol in Saccharomyces cerevisiae is metabolized in the present invention
It is specifically described in the method for wine brewing, those skilled in the art is it will be clearly understood that following specific descriptions are for the ease of reason
The solution present invention, is not intended to limit protection scope of the present invention.
In following embodiment, using the residual total sugar content in ethanol after the detection fermentation of DNS methods, the detection fermentation of Fei Linshi methods
Residual content of reducing sugar in ethanol afterwards, sample is prepared using density bottle method, then using alcoholometer (Qing County reflex glass instrument
Watch Factory's Ji word 09000124) wine degree is ethanol content in ethanol after measure fermentation.
In following embodiment, the producer of material therefor and specification are as shown in table 1
Material used and sale vendor name in the embodiment of the present invention of table 1
Saccharomyces cerevisiae 1522, category saccharomyces cerevisiae (Saccharomyces cerevisiae), its preparation technology flow are as follows：
Slant strains → F bottles culture → fastening tank culture → seed tank culture (2000L) → commodity tank culture (60m3)→
Centrifugation → press filtration → granulation → drying → finished product.
Zymotechnique main points control：
Fermentation medium components mainly have：Sucrose, yeast extract, peptone, magnesium sulfate, potassium dihydrogen phosphate, paste monosodium glutamate,
Calcium chloride, vitamin, penicillin etc..
Medium's PH Value control is 4.8~6.5, aerlbic culture.
In fermentation process, every a hour detection and fermentation index p H is recorded, temperature, molasses, nitrogen, phosphorus source stream dosage,
Bahrain is worth, weight in wet base, alcohol value and ventilation.
When somatic cells are uniform, inclusion is abundant, cellule is few, bud ratio is low, fermentation ends are represented, detect yeast cake
Moisture, protein, P2O5And trehalose.
For dried finished product, determining moisture, TCS and living cell rate, its index in time is：Dried product is done
Content of material 94~96%, living cell rate more than 65%, TCS >=20,000,000,000/gram.
Bacterial strain appraising datum is as follows：
It is sequenced using 16S rRNA and carries out bacterial strain identification, the results showed that sequence homology is more than 99%, it may be determined that this hair
New dry ferment 1522 in bright belongs to saccharomyces cerevisiae, and biological classification is saccharomyces cerevisiae (Saccharomyces
cerevisiae)。
Saccharomyces cerevisiae 1522 used has been described in the patent of Application No. 2015100830825 in the present invention.
Embodiment 1
50kg corns are weighed, removal of impurities crushes, and liquefaction processing, is cooled to 40 DEG C.In terms of base starting material dry weight, urine is separately added into
Plain 0.15g/100g, acid protease 10u/g, phytase 2u/g, potassium dihydrogen phosphate 0.01g/100g, sodium chloride 0.001g/
100g, fully mix.In terms of base starting material dry weight, 20u/g glucoamylases are added, are fully mixed.New dry ferment dosage
0.1g/100g, fully mix.Distilled water is added to material-water ratio 1:3.4, pH to 4.5 is adjusted, adjusts the temperature to 30 DEG C, regulation turn
Speed to 160rmp carry out alcoholic fermentation.10.4 ° of 68h measure fermented wines of fermenting part, residual reduced sugar 0.22%, residual total reducing sugar 1.29%.
Embodiment 2
The removal of impurities of 50kg corns is crushed, liquefaction processing, be cooled to 30 DEG C.In terms of base starting material dry weight, urea is separately added into
0.25g/100g, phytase 2u/g, cellulase 2u/g, hemicellulase 25u/g, zytase 3u/g, potassium dihydrogen phosphate
0.01g/100g, magnesium sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, 30u/g glucoamylases are added,
Fully mix.New dry ferment dosage 0.1g/100g, fully mix.Distilled water is added to material-water ratio 1:2.6, pH to 5 is adjusted,
Adjust the temperature to 36 DEG C, regulation rotating speed to 150rmp progress alcoholic fermentations.13.6 ° of 68h measure fermented wines of fermenting part, residual reduced sugar
0.28%, residual total reducing sugar 1.1%.
Embodiment 3
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 37 DEG C.In terms of base starting material dry weight, urea is separately added into
0.25g/100g, acid protease 5u/g, phytase 2u/g, cellulase 5u/g, hemicellulase 20u/g, zytase
10u/g, dipotassium hydrogen phosphate 0.01g/100g, zinc sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, add
45u/g glucoamylases, fully mix.New dry ferment dosage 0.2g/100g, fully mix.Distilled water is added to expecting water
Than 1:2.6, pH to 5.3 is adjusted, adjusts the temperature to 35 DEG C, regulation rotating speed to 180rmp progress alcoholic fermentations.Ferment 68h measure hairs
13.6 ° of ferment wine part, residual reduced sugar 0.27%, residual total reducing sugar 1.11%.
Embodiment 4
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 40 DEG C.In terms of base starting material dry weight, urea is separately added into
0.1g/100g, acid protease 25u/g, phytase 5u/g, cellulase 10u/g, zytase 3u/g, dipotassium hydrogen phosphate
0.01g/100g, potassium chloride 0.001g/100g, fully mix.In terms of base starting material dry weight, 45u/g glucoamylases are added,
Fully mix.New dry ferment dosage 0.3g/100g, fully mix.Distilled water is added to material-water ratio 1:2.6, regulation pH to
4.9, adjust the temperature to 33 DEG C, regulation rotating speed to 170rmp progress alcoholic fermentations.13.6 ° of 68h measure fermented wines of fermenting part is residual to go back
Raw sugar 0.24%, residual total reducing sugar 1.13%.
Embodiment 5
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.8g/100g, acid protease 6u/g, phytase 2u/g, hemicellulase 1u/g, zytase 1u/g, dipotassium hydrogen phosphate 1g/
100g, sodium chloride 0.004g/100g, zinc sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, 5u/g Portugals are added
Grape saccharogenic amylase, fully mix.New dry ferment dosage 0.05g/100g, fully mix.Distilled water is added to material-water ratio 1:5,
PH to 4.7 is adjusted, 33 DEG C of temperature of regulation, regulation rotating speed to 160rmp, carries out alcoholic fermentation.The 68h that ferments determines fermented wine part
7.3 °, residual reduced sugar 0.12%, residual total reducing sugar 0.82%.
Embodiment 6
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
1g/100g, acid protease 25u/g, phytase 5u/g, cellulase 10u/g, zytase 1u/g, potassium dihydrogen phosphate 0.5g/
100g, potassium chloride 0.004g/100g, magnesium sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, 50u/g is added
Glucoamylase, fully mix.New dry ferment dosage 0.5g/100g, fully mix.Distilled water is added to material-water ratio 1:2,
PH to 4.7 is adjusted, 33 DEG C of temperature of regulation, regulation rotating speed to 160rmp, carries out alcoholic fermentation.The 68h that ferments determines fermented wine part
16.8 °, residual reduced sugar 0.35%, residual total reducing sugar 2.32%.
Embodiment 7
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.5g/100g, phytase 5u/g, cellulase 1u/g, hemicellulase 24u/g, potassium dihydrogen phosphate 0.05g/100g, magnesium sulfate
1g/100g, fully mix.In terms of base starting material dry weight, 30u/g glucoamylases are added, are fully mixed.New dry ferment is used
0.5g/100g is measured, is fully mixed.Distilled water is added to material-water ratio 1:3, adjust pH to 4.7,33 DEG C of temperature of regulation, regulation rotating speed
To 160rmp, alcoholic fermentation is carried out.13.2 ° of 68h measure fermented wines of fermenting part, residual reduced sugar 0.25%, residual total reducing sugar 1.69%.
Embodiment 8
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.5g/100g, acid protease 16u/g, phytase 4u/g, cellulase 10u/g, hemicellulase 10u/g, zytase
10u/g, potassium dihydrogen phosphate 1g/100g, magnesium sulfate 1g/100g, fully mix.In terms of base starting material dry weight, 50u/g grapes are added
Saccharogenic amylase, fully mix.New dry ferment dosage 0.5g/100g, fully mix.Distilled water is added to material-water ratio 1:3, regulation
PH to 4.7,33 DEG C of temperature of regulation, regulation rotating speed to 160rmp, carries out alcoholic fermentation.13.3 ° of 68h measure fermented wines of fermenting part,
Residual reduced sugar 0.23%, residual total reducing sugar 1.59%.
Above example shows, makes new saccharomyces cerevisiae pre- by additionally adding nitrogen source, phosphorus source, enzyme preparation, inorganic salts
Alcohol high gravity fermentation is completed in fixing time, reduces glucoamylase relative usage, reduces production cost.
Comparative example 1
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.25g/100g, phytase 1u/g, cellulase 2u/g, hemicellulase 2u/g, zytase 3u/g, potassium dihydrogen phosphate
0.01g/100g, magnesium sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, 30u/g glucoamylases are added,
Fully mix.New dry ferment dosage 0.003g/100g, fully mix.Distilled water is added to material-water ratio 1:3, regulation pH to
4.7,33 DEG C of temperature, rotating speed to 160rmp, carry out alcoholic fermentation.10.5 ° of 68h measure fermented wines of fermenting part, residual reduced sugar
6.21%, residual total reducing sugar 8.21%.
Yeast dosage is few, fermenting power deficiency, it is impossible to made full use of to reduced sugar；Secondly the saccharification enzyme amount of secretion is few, influences
Saccharifying, cause fermentation it is not thorough, residual reduced sugar, residual total reducing sugar are higher.
Comparative example 2
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.25g/100g, phytase 5u/g, cellulase 25u/g, hemicellulase 20u/g, zytase 5u/g, potassium dihydrogen phosphate
0.01g/100g, magnesium sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, 30u/g glucoamylases are added,
Fully mix.New dry ferment dosage 0.003g/100g, fully mix.Distilled water is added to material-water ratio 1:3, regulation pH to
4.7,33 DEG C of temperature, rotating speed to 160rmp, carry out alcoholic fermentation.10.5 ° of 68h measure fermented wines of fermenting part, residual reduced sugar
6.50%, residual total reducing sugar 8.07%.
Yeast dosage causes fermentation not thorough less, residual reduced sugar, the problem of residual total reducing sugar is higher, is adding excessive enzyme preparation
It is difficult to solve.
Comparative example 3
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.5g/100g, acid protease 30u/g, phytase 5u/g, cellulase 10u/g, potassium dihydrogen phosphate 0.01g/100g, sulfuric acid
Magnesium 0.001g/100g, fully mix.In terms of base starting material dry weight, 18u/g glucoamylases are added, are fully mixed.Novel dry
Yeast dosage 0.2g/100g, fully mix.Distilled water is added to material-water ratio 1:3, pH to 4.7 is adjusted, 33 DEG C of temperature, rotating speed are extremely
160rmp, carry out alcoholic fermentation.11.8 ° of 68h measure fermented wines of fermenting part, residual reduced sugar 1.6%, residual total reducing sugar 2.85%.
The enzyme preparations such as acid protease are excessively added, and not only increase cost, it is also possible to are suppressed yeast alcoholic fermentation process, made
It must ferment not thorough.
Comparative example 4
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.5g/100g, acid protease 2u/g, phytase 2u/g, cellulase 3u/g, hemicellulase 35u/g, zytase 10u/
G, potassium dihydrogen phosphate 0.01g/100g, magnesium sulfate 0.001g/100g, fully mix.In terms of base starting material dry weight, 55u/g is added
Glucoamylase, fully mix.New dry ferment dosage 0.5g/100g, fully mix.Distilled water is added to material-water ratio 1:3,
PH to 4.7 is adjusted, 33 DEG C of temperature, rotating speed to 160rmp, carries out alcoholic fermentation.12.0 ° of 68h measure fermented wines of fermenting part is residual to go back
Raw sugar 1.4%, residual total reducing sugar 2.68%.
Glucoamylase, hemicellulase etc. excessively add, and not only increase cost, it is also possible to suppress yeast alcoholic fermentation
Process so that fermentation is not thorough.
Comparative example 5
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.5g/100g, phytase 2u/g, zytase 15u/g, potassium dihydrogen phosphate 0.01g/100g, magnesium sulfate 0.001g/100g, fill
Divide and mix.In terms of base starting material dry weight, 30u/g glucoamylases are added, are fully mixed.New dry ferment dosage 0.5g/
100g, fully mix.Distilled water is added to material-water ratio 1:3, pH to 4.7 is adjusted, 33 DEG C of temperature, rotating speed to 160rmp, enters to serve a round of liquor to the guests
Essence fermentation.12.4 ° of 68h measure fermented wines of fermenting part, residual reduced sugar 0.29%, residual total reducing sugar 4.69%.
The enzyme preparations such as zytase are excessively added, and not only increase cost, it is also possible to suppress yeast alcoholic fermentation process so that
Fermentation is not thorough.
Comparative example 6
The removal of impurities of 100kg corns is crushed, liquefaction processing, be cooled to 35 DEG C.In terms of base starting material dry weight, urea is separately added into
0.1g/100g, acid protease 3u/g, phytase 2u/g, dipotassium hydrogen phosphate 0.01g/100g, magnesium sulfate 0.001g/100g, fill
Divide and mix.In terms of base starting material dry weight, 4u/g glucoamylases are added, are fully mixed.New dry ferment dosage 0.04g/
100g, fully mix.Distilled water is added to material-water ratio 1:2.6, pH to 4.7 is adjusted, 33 DEG C of temperature, rotating speed to 160rmp, is carried out
Alcoholic fermentation.11.4 ° of 68h measure fermented wines of fermenting part, residual reduced sugar 0.4%, residual total reducing sugar 5.23%.
Glucoamylase is very few, influences the saccharification of base starting material, makes fermentation not thorough, and residual total reducing sugar is higher.
Claims (14)
1. a kind of fermentative production of ethanol saccharomyces cerevisiae composition, it is characterised in that it contains nitrogen source, phosphorus source material, enzyme
Preparation, inorganic salts, glucoamylase and saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae), the saccharomyces cerevisiae
China typical culture collection center is preserved in, deposit number is CCTCC No.M2014657, and the enzyme preparation does not include grape
Saccharogenic amylase.
2. a kind of fermentative production of ethanol saccharomyces cerevisiae feedstock composition, it is characterised in that it contains fermentative production of ethanol base
Matter raw material, nitrogen source, phosphorus source material, enzyme preparation, inorganic salts, glucoamylase and saccharomyces cerevisiae 1522
(Saccharomyces cerevisiae), the saccharomyces cerevisiae is preserved in China typical culture collection center, deposit number
For CCTCC No.M2014657, the enzyme preparation does not include glucoamylase, and the base starting material is preferably starchy material
And/or molasses matter raw material.
3. composition according to claim 1 or 2, wherein, the starchy material be selected from corn, tapioca starch, sorghum or
It is one or more of in cereal.
4. composition according to claim 1 or 2, wherein, relative to fermentative production of ethanol base starting material gross dry weight amount,
Following substances content is respectively：0.1~1g/100g of nitrogen source, preferably 0.15-1g/100g, 0.01~1g/100g of phosphorus source, enzyme preparation
10~50u/g, 0.001~1g/100g of inorganic salts, 5~50u/g of glucoamylase and saccharomyces cerevisiae 1522
(Saccharomyces cerevisiae) 0.05~0.5g/100g, preferably 0.1-0.5g/100g, wherein the saccharomyces cerevisiae
Weight be yeast dry weight.
5. composition according to claim 4, wherein, enzyme preparation contain phytase 2-5u/g and selected from acid protease,
At least one of cellulase, hemicellulase and zytase enzyme 8-45u/g, wherein other beyond above-mentioned phytase
Enzyme content is：Acid protease 0-25u/g, preferably 1-25u/g, preferably cellulase 0-10u/g, 1-10u/g, hemicellulase
0-25u/g, preferably 1-25u/g and zytase 0-10u/g, preferably 1-10u/g.
6. composition according to claim 5, wherein, relative to fermentative production of ethanol base starting material gross dry weight amount, under
Stating content of material is respectively：Nitrogen source 0.15-0.25g/100g, 0.01~1g/100g of phosphorus source, 30~45u/g of enzyme preparation, inorganic salts
0.001~1g/100g, 30~45u/g of glucoamylase and saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae)
0.1~0.3g/100g.
7. composition according to claim 6, wherein, relative to fermentative production of ethanol base starting material gross dry weight amount, institute
State enzyme preparation and contain following components：Acid protease 5-25u/g, phytase 2-5u/g, cellulase 2-10u/g, hemicellulose
Enzyme 20-25u/g and zytase 3-10u/g.
8. according to the composition described in claim any one of 1-7, wherein, nitrogen source be selected from urea, ammonium sulfate, ammonium di-hydrogen phosphate,
One or two or more kinds in the hydrogen ammonia of phosphoric acid one；Phosphorus source is selected from potassium dihydrogen phosphate and/or dipotassium hydrogen phosphate；Enzyme preparation is selected from acidity
Two kinds in protease, phytase, cellulase, hemicellulase or zytase or more than three kinds；Inorganic salts are selected from sulfuric acid
One kind in ammonium, zinc sulfate, magnesium sulfate, potassium chloride, sodium chloride, ferrous sulfate, copper sulphate, calcium chloride or manganese sulfate or two kinds
More than.
9. a kind of method that ethanol is produced using fermentation by saccharomyces cerevisiae, wherein, using the raw material described in claim any one of 2-8
Composition carries out fermenting and producing, comprises the following steps：
Into the feedstock composition described in claim any one of 2-8, add water and obtain mixture, carry out fermentative production of ethanol.
10. the method according to claim 11, wherein, according to material water weight ratio 1：(2-5) adds water.
11. according to the method described in claim any one of 9-10, wherein, after adding water, adjust the mixture ph to
4.5-5.3 carries out fermentative production of ethanol.
12. according to the method described in claim any one of 9-11, wherein, after adding water, the temperature for adjusting the mixture is
32-36 DEG C, preferably 32-33 DEG C, carry out fermentative production of ethanol.
13. according to the method described in claim any one of 9-12, wherein, after adding water, mixture stirring is sent out
Ferment produces ethanol, and the speed of agitator is 150-180rmp.
14. a kind of application of saccharomyces cerevisiae 1522 (Saccharomyces cerevisiae) in fermentative production of ethanol, it is special
Sign is that it is preserved in China typical culture collection center, and deposit number is CCTCC No.M2014657.
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| CN109576311A (en) * | 2018-11-14 | 2019-04-05 | 中粮生化能源（肇东）有限公司 | A kind of method and alcohol of fermenting and producing alcohol |
| CN111040982A (en) * | 2019-12-12 | 2020-04-21 | 广西乐酵生物科技有限公司 | Saccharomyces cerevisiae promoter and preparation method and application process thereof |
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| CN102154382A (en) * | 2011-01-11 | 2011-08-17 | 南阳天冠生物发酵有限公司 | Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae |
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| CN102154382A (en) * | 2011-01-11 | 2011-08-17 | 南阳天冠生物发酵有限公司 | Method for producing cellulosic ethanol by using 1308 Saccharomyces cerevisiae |
| CN103184169A (en) * | 2011-12-31 | 2013-07-03 | 安琪酵母股份有限公司 | Cellulose yeast, double-strain saccharomyces cerevisiae composition and fermentation method of cellulosic ethanol |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109576311A (en) * | 2018-11-14 | 2019-04-05 | 中粮生化能源（肇东）有限公司 | A kind of method and alcohol of fermenting and producing alcohol |
| CN111040982A (en) * | 2019-12-12 | 2020-04-21 | 广西乐酵生物科技有限公司 | Saccharomyces cerevisiae promoter and preparation method and application process thereof |
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