CN107764880A - A kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker - Google Patents
A kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker Download PDFInfo
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- CN107764880A CN107764880A CN201710941266.XA CN201710941266A CN107764880A CN 107764880 A CN107764880 A CN 107764880A CN 201710941266 A CN201710941266 A CN 201710941266A CN 107764880 A CN107764880 A CN 107764880A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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Abstract
A kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker, it includes one piece of substrate, being prepared on substrate by chemistry coating or electrochemical plating processes has at least one shape thin layer electrode, and at least one layer of contain with miocardial infarction protein marker antigen interactions, so as to producing the antibody reagent layer of detected electric signal is formed with the thin layer electrode；The antibody reagent layer is to be bonded in thin layer electrode surface by inorganic gel layer；Nano-particle is also bonded with above described thin layer electrode and contains layer, and a laminated body is collectively constituted with antibody reagent layer；Described nano-particle contains layer by being made up in electrochemistry of inert high-molecular compound；Described thin layer electrode is one of metal level, carbon electrode layer and silver electrode layer；It has the characteristics that simple in construction, easy to make, cost is lower and high sensitivity.
Description
Technical field
The present invention relates to a kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker, belong to
Biosensor technology field.
Background technology
Miocardial infarction refers to the ischemic necrosis of cardiac muscle, is the blood flow coronarius on the basis of coronary artery pathological changes
Drastically reduce or interrupt, corresponding cardiac muscle serious and enduringly acute ischemia is occurred, ultimately result in the ischemic necrosis of cardiac muscle,
Turn into and have a strong impact on one of angiocardiopathy of human health.The patient of acute myocardial infarction occurs, clinically often has
Have an intense pain, generate heat after lasting breastbone, white blood cell count(WBC) increases, Serum fibrosis markers raise and electrocardiogram reflecting myocardium is acute
The series of features of damage, ischemic and necrosis develops, and may occur in which arrhythmia cordis, shock or heart failure.Acute myocardial obstructs
Plug is substantially exactly the ischemic necrosis of cardiac muscle, and its fundamental cause is exactly coronary atherosclerosis, and it is narrow to be gradually developed to tube chamber
Narrow or even blocking, occurs thrombus suddenly on this basis, causes coronary artery to block completely caused.Rapid heart rate is not normal, acute
Left heart failure easily causes sudden death, and therefore, treatment is more early, and chance for survival is bigger, early rescue even 10 minutes 1 hour, has
May be continued the life of a people.
The diagnostic criteria of China's miocardial infarction includes at present：Clinical diagnosis, electrocardiographic abnormality and protein marker are containing measurement
Two in fixed three；But because about 25% infraction morbidity early stage is without typical clinical symptoms, about 50%
Patient's electrocardiogram is without typical change, if relying solely on ECG change and clinical symptoms, the diagnostic accordance rate of miocardial infarction is only
75%.The advantages of protein marker measure is with high specificity, high sensitivity；So in recent years for miocardial infarction protein markers
The research of thing is very active.The protein marker of miocardial infarction mainly includes myoglobins, creatine kinase isozyme and flesh calcium egg
White I.
Existing protein markers object detecting method mainly has ELISA（ELISA）, immunofluorescence technique（IFA）With it is immune
Colloidal gold method.ELISA and IFA have high specificity, high sensitivity, it is reproducible the advantages of, but its operation sequence is complicated, can only
Carried out in laboratory；Professional is needed to operate, it is required costly with special instrument and equipment, detection time length.While one
Secondary response can only detect an index.Colloidal Gold tests simple and quick, high sensitivity, and cost is low, and can once survey
Try several marks.But its high sensitivity is easily caused false positive results, quality control is also unstable.Therefore develop and using joint
Detection is reality problem in the urgent need to address to improve the sensitivity of diagnosis of myocardial infarction and specificity and positive predictive value.
The content of the invention
It is an object of the invention to overcome the shortcomings of the prior art, and provide it is a kind of it is simple in construction, easy to make, into
Biosensor thin layer electrode of this lower and high sensitivity current mode miocardial infarction protein marker and preparation method thereof.
The purpose of the present invention is completed by following technical solution, a kind of quick detection miocardial infarction protein marker
Biosensor thin layer electrode, it includes one piece of substrate, and being prepared on substrate by chemistry coating or electrochemical plating processes has
At least one shape thin layer electrode, at least one layer of contain and miocardial infarction protein marker antigen phase is formed with the thin layer electrode
Interaction, so as to producing the antibody reagent layer of detected electric signal；The antibody reagent layer is glued by inorganic gel layer
Close on thin layer electrode surface.
As preferred：Nano-particle is also bonded with above described thin layer electrode and contains layer, and it is common with antibody reagent layer
Form a laminated body；Described nano-particle contains layer by being made up in electrochemistry of inert high-molecular compound；Described is thin
Layer electrode is one of metal level, carbon electrode layer and silver electrode layer.
The present invention utilizes miocardial infarction protein marker antigen and corresponding antibodies phase interaction on biosensor thin layer electrode
With so as to produce the electric signal that can be detected in interaction process；Therefore, biology sensor electricity electricity of the present invention
Pole, the detection resisted for miocardial infarction protein marker is high specific, and the sensitivity of its detection is very high, repeatability
Very well；Have the characteristics that simple in construction, easy to make, cost is lower and high sensitivity.
Brief description of the drawings
Fig. 1 is the positive structure schematic of thin layer electrode of the present invention.
Fig. 2 is the side structure schematic view of thin layer electrode of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be described in detail：Fig. 1, shown in 2, a kind of quick detection of the present invention
The biosensor thin layer electrode of miocardial infarction protein marker, it includes one piece of substrate 1, on substrate 1 by chemistry coating or
Prepared by electrochemical plating processes have at least one shape thin layer electrode 2, and at least one layer is formed with the thin layer electrode 2 and is contained and the heart
Muscle infarction protein marker antigen interactions, so as to producing the antibody reagent layer 3 of detected electric signal；The antibody examination
Oxidant layer 3 is to be bonded in the surface of thin layer electrode 2 by inorganic gel layer 4.
As preferable one embodiment：Described thin layer electrode 2 is also bonded with nano-particle above and contains layer 5, and with
Antibody reagent layer 3 collectively constitutes a laminated body；Described nano-particle contains layer 5 by being inert macromolecule chemical combination in electrochemistry
Thing is made；Described thin layer electrode 2 is one of metal level, carbon electrode layer and silver electrode layer.
The electrode that the present invention is prepared by chemical plating, electrochemical plating processes；Can qualitative and quantitative detection, favorable reproducibility,
The advantages of specific height, service life are grown, and stability is good.
It is therefore, above-mentioned not refer to and undocumented technology is existing skill the invention belongs to improvements over the prior art
Art, and skillfully grasped for those skilled in the art, and on the basis of technology disclosed above, can easily implement this hair
It is bright.
Claims (2)
1. a kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker, it includes one piece of substrate, substrate
Upper prepared by chemistry coating or electrochemical plating processes has at least one shape thin layer electrode, it is characterised in that：In the thin layer
At least one layer of contain with miocardial infarction protein marker antigen interactions, so as to producing detected telecommunications is formed with electrode
Number antibody reagent floor；The antibody reagent layer is to be bonded in thin layer electrode surface by inorganic gel layer.
2. the biosensor thin layer electrode of quick detection miocardial infarction protein marker according to claim 1, it is special
Sign is that being also bonded with nano-particle above described thin layer electrode contains layer, and collectively constitutes a lamination with antibody reagent layer
Body；Described nano-particle contains layer by being made up in electrochemistry of inert high-molecular compound；Described thin layer electrode is gold
Belong to one of layer, carbon electrode layer and silver electrode layer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710941266.XA CN107764880A (en) | 2017-10-11 | 2017-10-11 | A kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker |
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| CN201710941266.XA CN107764880A (en) | 2017-10-11 | 2017-10-11 | A kind of biosensor thin layer electrode of quick detection miocardial infarction protein marker |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109406797A (en) * | 2018-12-26 | 2019-03-01 | 浙江海洋大学 | A kind of method of quick detection myocardial infarction albumen and accounting marker |
Citations (7)
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| CN1462878A (en) * | 2003-06-20 | 2003-12-24 | 复旦大学 | Sensor of chip contaonong microelectrode array |
| CN1588028A (en) * | 2004-08-26 | 2005-03-02 | 复旦大学 | Nano sol-gel film electrode, its preparing method and use |
| CN101178375A (en) * | 2007-12-10 | 2008-05-14 | 杨星群 | Manufacturing method of biology enzymic electrode used for detecting pesticide residue |
| CN101413890A (en) * | 2008-12-03 | 2009-04-22 | 吉林大学 | Sensing element based on titanic oxide embedding technology and manufacturing method thereof |
| JP2009085813A (en) * | 2007-10-01 | 2009-04-23 | Panasonic Corp | Immunochromatographic testpiece and measurement method using the same |
| CN104020202A (en) * | 2014-06-27 | 2014-09-03 | 中国科学院苏州生物医学工程技术研究所 | Method for detecting content of clenbuterol in solution to be detected |
| CN106841617A (en) * | 2017-02-20 | 2017-06-13 | 济南大学 | A kind of preparation method and application of electrochemical luminescence immunosensor sensing interface |
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2017
- 2017-10-11 CN CN201710941266.XA patent/CN107764880A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1462878A (en) * | 2003-06-20 | 2003-12-24 | 复旦大学 | Sensor of chip contaonong microelectrode array |
| CN1588028A (en) * | 2004-08-26 | 2005-03-02 | 复旦大学 | Nano sol-gel film electrode, its preparing method and use |
| JP2009085813A (en) * | 2007-10-01 | 2009-04-23 | Panasonic Corp | Immunochromatographic testpiece and measurement method using the same |
| CN101178375A (en) * | 2007-12-10 | 2008-05-14 | 杨星群 | Manufacturing method of biology enzymic electrode used for detecting pesticide residue |
| CN101413890A (en) * | 2008-12-03 | 2009-04-22 | 吉林大学 | Sensing element based on titanic oxide embedding technology and manufacturing method thereof |
| CN104020202A (en) * | 2014-06-27 | 2014-09-03 | 中国科学院苏州生物医学工程技术研究所 | Method for detecting content of clenbuterol in solution to be detected |
| CN106841617A (en) * | 2017-02-20 | 2017-06-13 | 济南大学 | A kind of preparation method and application of electrochemical luminescence immunosensor sensing interface |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109406797A (en) * | 2018-12-26 | 2019-03-01 | 浙江海洋大学 | A kind of method of quick detection myocardial infarction albumen and accounting marker |
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