CN107760748A - A kind of industrialized production walnut active peptide and preparation method - Google Patents
A kind of industrialized production walnut active peptide and preparation method Download PDFInfo
- Publication number
- CN107760748A CN107760748A CN201711067185.8A CN201711067185A CN107760748A CN 107760748 A CN107760748 A CN 107760748A CN 201711067185 A CN201711067185 A CN 201711067185A CN 107760748 A CN107760748 A CN 107760748A
- Authority
- CN
- China
- Prior art keywords
- walnut
- active peptide
- preparation
- peptide
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention discloses a kind of industrialized production walnut active peptide and preparation method.The present invention is digested using walnut as raw material using flavor protease, pepsin and neutral proteinase multienzyme, and then by processes such as enzyme deactivation, centrifugation, filtering, concentration, decolouring and spray drying, walnut active peptide is made.Component of its molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and tetrapeptide valyltryptophanyllysine isoleucine serine (Val Trp Ile Ser, VWIS) sequence peptide fragment at least containing 3% content.The production method of the present invention has bioactivity height, processing cost is low, treating capacity is big, is easy to the advantages of industrialized production, the walnut active peptide prepared in this approach can be applied to the exploitation and production of ordinary food, health food and medicine as new functional nutrient composition, have wide market prospects.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of industrialized production walnut active peptide and preparation method.
Background technology
Walnut also known as apricot are real.Walnut is rich in protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, dimension life
The nutritional ingredient such as plain P and calcium, phosphorus, iron.The content of wherein carrotene is only second to mango in fruit, and walnut is referred to as by people
The fruit of anticancer.Walnut contains abundant fat oil, plays the role of to reduce cholesterol, and therefore, walnut is to preventing and treating cardiovascular system disease
Disease plays the role of good；Chinese medicine theory thinks that walnut has the effect of promoting the production of body fluid to quench thirst, moistening lung Dingchuan, is usually used in dryness of the lung asthma
The health care and treatment of the patients such as cough.The newest research results of American researcher show that cholesterol levels are normal or slightly higher
People, the low nutrition dense food in its meals can be substituted with walnut, reaching reduces blood cholesterol levels and keep health of heart
Purpose.
Application number CN201410386832.1 is disclosed " a kind of preparation method of walnut polypeptide beverage ".It is main to include removing
The scarlet of walnut kernel；Walnut oil and walnut dregs are made；Defibrination obtains walnut protein stoste, screenings separation, homogenization, obtains core
Peach protein liquid；Enzymolysis；Enzyme deactivation, obtain walnut polypeptide solution；7th, auxiliary material is added into walnut polypeptide solution, adds water constant volume laggard
Row homogenization, obtains walnut polypeptide beverage.Do not carry out the operations such as various filterings to enzymolysis liquid during peptide is obtained, can be residual
Partial impurities and purity and activity and mouthfeel to peptide is stayed to have an impact.
By carrying out protease hydrolyzed to walnut powder, the oligopeptide by proteolysis into small-molecular-weight, production is considerably increased
The dissolubility of product, the success of walnut active peptide are prepared as traditional food walnut powder deep processing of farm products and provide an infant industry
Change road, this abundant ordinary food protein resource of walnut powder is fully used, expand walnut powder significantly as one
The application field of the traditional ordinary food raw material of kind, improves the added value of large agricultural product, has played bigger economic value, and
Improve comprehensive social benefit.Compared with former albumen, the walnut active peptide of small molecule is more prone to be digested absorption.Due to it
A series of good food processing properties, walnut active peptide can also mix with the protein of other separate sources, to improve its battalion
Support value, it can also be used to produce the diversified food such as instant drink, drinks, meat products, dairy products, bakery product.With
The development of science and technology, the application of protein engineering, biologically active peptide and its protolysate are produced using zymolysis technique
Research and product development have become focus and main flow, and enzymolysis walnut protein produces the existing portion of laboratory technique document of polypeptide
Point, but the preparation method for producing walnut peptide in industry does not have also.
The content of the invention
Present invention aims at a kind of preparation method of walnut active peptide is provided, to solve technical problem.
A kind of preparation method of walnut active peptide, comprises the following steps：
A, walnut is pre-processed, carries out ultramicro grinding, 200-600 mesh is crossed, with 100: 2~10 (L: kg) liquid ratio water
Sized mixing with walnut powder mixing, adjust the micro- basifications of pH 8-9, be heated to 60~80 DEG C.30~120min of insulated and stirred；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, by slag charge plus
Water dilutes, and is heated to 60~80 DEG C, stirs and separate, and is carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 4-6, mechanical agitation 8-12h, centrifugation, collects precipitation, precipitation as walnut egg
In vain, it is repeated twice；
D, precipitation plus water are redissolved, is heated to 75-100 DEG C, time 15-45min so that protein denaturation, then carry out
It is high-pressure homogeneous；
E, above-mentioned pH value of solution is adjusted to 5-10, adds special enzyme preparation: flavor protease: pepsin: neutral proteinase
For 5~7: 2~3: 3~5, hydrolysis temperature is 40-65 DEG C, time 1.5-8h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：For the centrifugation of thick enzymolysis liquid, filtering, evaporation and concentration, addition activated carbon decolorizing, then spray drying are obtained into walnut
Active peptide powder.
The preparation method of walnut active peptide described above, it is characterised in that in described step A, carrying out ultramicro grinding can
To improve enzymatic hydrolyzation, material composition is made full use of.
The preparation method of walnut active peptide described above, it is characterised in that in described step C, using the side of thermal denaturation
Method, it can be more favorable for digesting, enzymolysis is more abundant.High-pressure homogeneous, further raising enzymolysis effect is carried out after thermal denaturation simultaneously
Rate.
The preparation method of walnut active peptide described above, it is characterised in that described step D, special enzyme preparation combination bag
Include flavor protease: pepsin: neutral proteinase is 5~7: 2~3: 3~5.Research finds the enzyme of its special enzyme preparation combination
Solve the enzymolysis efficiency of the long-range about single enzyme of efficiency.
The preparation method of walnut active peptide described above, it is characterised in that being done for middle cold nebulization used in being spray-dried
Dry tower, further retain thermal activity material, and middle low-temperature spray drying tower is to be applied first in peptide technique.
A kind of preparation method of walnut active peptide, it is characterised in that described according to the preparation method described in claim 1
Walnut oligopeptide is characterized in that component of the molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and
Tetrapeptide valine-tryptophan-isoleucine-serine (Val-Trp-Ile-Ser, VWIS) sequence at least containing 3% content
Peptide fragment.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Embodiment
Embodiments of the invention are described in detail below, but the present invention can be limited and covered according to claim
Multitude of different ways implement.
1st, a kind of preparation method of walnut active peptide, comprises the following steps：
A, walnut is pre-processed, carries out ultramicro grinding, 200-600 mesh is crossed, with 100: 7 (L: kg) liquid ratio water and core
Peach powder mixing is sized mixing, and is adjusted 9 micro- basifications of pH, is heated to 70 DEG C.Insulated and stirred 80min；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, by slag charge plus
Water dilutes, and is heated to 70 DEG C, stirs and separate, and is carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 5, mechanical agitation 10h, centrifugation, collects precipitation, precipitation is walnut protein, heavy
Again twice；
D, precipitation plus water are redissolved, is heated to 85 DEG C, time 30min so that protein denaturation, it is equal then to enter horizontal high voltage
Matter；
E, above-mentioned pH value of solution is adjusted to 6, adds special enzyme preparation: flavor protease: pepsin: neutral proteinase is
6.3: 2.5: 4, flavor protease: pepsin: the addition of neutral proteinase is every gram of unit of protein 2000, hydrolysis temperature
For 50 DEG C, time 3h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：To centrifuge, filtering by thick enzymolysis liquid, filtered using 0.05~0.1 μm of the membrane filter system in aperture, condition
For 0.2~0.4MPa of pressure, 30~80 DEG C of temperature.It is concentrated by evaporation, adds activated carbon decolorizing, adds concentrate total solids content
5% quality of activated carbon is decolourized, and then spray drying obtains walnut active peptide powder.
2nd, the physics and chemistry composition of walnut active peptide and molecular weight distribution analysis
The composition analysis result of walnut active peptide is shown in Table 1, it can be seen that the total egg of walnut active peptide prepared by the present invention
White matter content is up to 98.2%, has very high product quality.
Table 1：The basic physical and chemical composition analysis of walnut active peptide
| Content % | |
| Gross protein (butt) | 98.2±0.35 |
| Fat | 0.08±0.01 |
| Ash content | 4.25±0.15 |
Walnut active peptide sample after sample introduction, is obtained its gel chromatography figure, handled with liquid data in GEL-HPLC
The gel chromatography data of walnut active peptide are substituted into calibration curve equation and calculated by software, obtain relative point of the peptide of sample
Protonatomic mass and its distribution.The absorption rate pole in human body of dipeptides, tripeptides in molecular weight 1000Da following components
Height, there is the higher nutritive value of specific ionization amino acid and physiological function.The present invention calculates walnut with areas of peak normalization method
The relative molecular mass distribution scope of active peptide, as shown in table 2.Molecular weight is less than it can be seen from molecular weight distribution result
1000Da component accounts for 91.49% altogether, if calculated by the mean molecule quantity 137Da of amino acid, molecular weight 1000Da with
Under component it is then mostly be oligopeptide below octapeptide, also including part free amino acid.Its middle-molecular-weihydroxyethyl is 140Da-500Da's
60.94% is accounted for, occupies below the 1000Da overwhelming majority, this part is mainly dipeptides, tripeptides and tetrapeptide.
Table 2：Walnut bioactive peptide molecule amount distribution results
3rd, Structural Identification quantifies with significant peptide fragment in walnut active peptide
Shown according to domestic and international newest nutrient research, after protein intake, be not fully hydrolyzed into amino acid, but big portion
Divide and digested and assimilated in the form of peptide by body.Peptide matters are in addition to trophic function that is easy to digest, easily absorbing, prior life
Thing meaning, which is mainly reflected in it, has the incomparable physiological active functionses of amino acid.Walnut active peptide is as a kind of Combination
Peptide, contain many peptide fragments with various physiological functions.Meanwhile its relative molecular mass less than 1000 component account for 90% with
On, illustrate that this product is substantially made up of small peptide.Special protein raw materials source and unique production technology, contain this product
There is a certain amount of characteristic component peptide.
The peptide fragment in walnut active peptide is separated for we and Structural Identification.By using high performance liquid chromatography separation with
Mass spectral analysis, is separated and Structural Identification to the peptide fragment in this product, identifies the primary structure of 40 peptide fragments altogether.
We have worked out tetrapeptide valine-tryptophan-isoleucine-serine in quantitative determination walnut active peptide
The high efficiency liquid phase chromatographic analysis method of (Val-Trp-Ile-Ser, VWIS).By walnut active peptide sample after pre-treatment, with anti-phase
C18 fillers are stationary phase, and the difference according to sample component molecular polarity size is separated, in UV absorption wavelength 220nm bars
Detect under part, quantified using external standard method, and chromatogram and its data are handled, VWIS content is calculated.Mirror
Containing more than 3% VWIS in the walnut active peptide sample of different batches, therefore using VWIS as the spy in walnut active peptide
Sign property composition.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (5)
1. a kind of preparation method of walnut active peptide, it is characterised in that comprise the following steps：
A, walnut is pre-processed, carries out ultramicro grinding, 200-600 mesh is crossed, with 100: 2~10 (L: kg) liquid ratio water and core
Peach powder mixing is sized mixing, and is adjusted the micro- basifications of pH 8-9, is heated to 60~80 DEG C.30~120min of insulated and stirred；
B, the alkaline feed liquid in retort is pumped into centrifuge, is separated into clear liquid and slag charge, collect slag charge, slag charge plus water is dilute
Release, be heated to 60~80 DEG C, stir and separate, carried out 3 times with same processing mode；
C, the pH value of supernatant is adjusted to after 4-6, mechanical agitation 8-12h, centrifugation, collects precipitation, precipitation is walnut protein, heavy
Again twice；
D, precipitation plus water are redissolved, is heated to 75-100 DEG C, time 15-45min so that protein denaturation, then enter horizontal high voltage
Homogeneous；
E, above-mentioned pH value of solution is adjusted to 5-10, adds special enzyme preparation：Flavor protease: pepsin: neutral proteinase be 5~
7: 2~3: 3~5, hydrolysis temperature is 40-65 DEG C, time 1.5-8h；
F, after enzymolysis terminates, 90-125 DEG C of enzyme-removal temperature, the enzyme deactivation time is 1-3h, and thick enzymolysis liquid is obtained after terminating；
G：For the centrifugation of thick enzymolysis liquid, filtering, evaporation and concentration, addition activated carbon decolorizing, then spray drying are obtained into walnut activity
Gly-His-Lys.
2. according to the method for claim 1, it is characterised in that flavor protease in the step E: pepsin: neutral
The addition of protease is every gram of unit of protein 1000~2000.
3. according to the method for claim 1, it is characterised in that in the step G, using 0.05~0.1 μm of the film in aperture
Filter plant is filtered, and condition is 0.2~0.4MPa of pressure, 30~80 DEG C of temperature.
4. according to the method for claim 1, it is characterised in that in the step G, add concentrate total solids content
5% quality of activated carbon is decolourized.
A kind of 5. preparation method of walnut active peptide, it is characterised in that according to the preparation method described in claim 1, the core
Peach oligopeptide is characterized in that component of the molecular weight less than 1000Da accounts for more than 90%, and free aminoacid content is less than 5%, and extremely
Few tetrapeptide valine-tryptophan-isoleucine-serine (Val-Trp-Ile-Ser, VWIS) sequence peptide containing 3% content
Section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711067185.8A CN107760748A (en) | 2017-11-02 | 2017-11-02 | A kind of industrialized production walnut active peptide and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711067185.8A CN107760748A (en) | 2017-11-02 | 2017-11-02 | A kind of industrialized production walnut active peptide and preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107760748A true CN107760748A (en) | 2018-03-06 |
Family
ID=61272539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711067185.8A Pending CN107760748A (en) | 2017-11-02 | 2017-11-02 | A kind of industrialized production walnut active peptide and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107760748A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110916140A (en) * | 2019-11-22 | 2020-03-27 | 河北绿蕾农林科技有限公司 | Special walnut meal replacement powder for middle-aged and old people and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101748180A (en) * | 2010-01-18 | 2010-06-23 | 山东世纪春食品有限公司 | Functional peanut small molecule mixed polypeptide, preparation method and application thereof |
| CN103173511A (en) * | 2011-12-23 | 2013-06-26 | 中国食品发酵工业研究院 | Method for industrially producing wheat glutamine peptide |
| CN103484518A (en) * | 2013-09-11 | 2014-01-01 | 邹远东 | Walnut protein peptide, preparation method and application thereof |
| CN104293870A (en) * | 2014-09-25 | 2015-01-21 | 汾州裕源土特产品有限公司 | Preparation method of antioxidant walnut polypeptide health product |
-
2017
- 2017-11-02 CN CN201711067185.8A patent/CN107760748A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101748180A (en) * | 2010-01-18 | 2010-06-23 | 山东世纪春食品有限公司 | Functional peanut small molecule mixed polypeptide, preparation method and application thereof |
| CN103173511A (en) * | 2011-12-23 | 2013-06-26 | 中国食品发酵工业研究院 | Method for industrially producing wheat glutamine peptide |
| CN103484518A (en) * | 2013-09-11 | 2014-01-01 | 邹远东 | Walnut protein peptide, preparation method and application thereof |
| CN104293870A (en) * | 2014-09-25 | 2015-01-21 | 汾州裕源土特产品有限公司 | Preparation method of antioxidant walnut polypeptide health product |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110916140A (en) * | 2019-11-22 | 2020-03-27 | 河北绿蕾农林科技有限公司 | Special walnut meal replacement powder for middle-aged and old people and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107668314A (en) | A kind of industrialized production pea active peptide and preparation method | |
| CN102048022B (en) | Soybean polypeptide without hydrolysis and bitter tastes as well as preparation method and application thereof | |
| CN102212107B (en) | Rice protein polypeptide and preparation method thereof | |
| CN102696767B (en) | A kind of deep working method fully utilizing walnut kernel production low fat Walnut Milk, active peptide, albumen powder | |
| CN104432388B (en) | Shiny-leaved yellowhorn peptide nutrient food and preparation method thereof | |
| CN104095243B (en) | Preparation technology for enzymolysis bioactive peptide nanometer selenium | |
| CN103109971A (en) | Method for preparing homoarginine walnut peptide | |
| CN106916871A (en) | A kind of bionic enzymatic prepares the production method of freshwater mussel meat small peptide | |
| CN103250865A (en) | Preparation method of peanut polypeptide | |
| CN109527162A (en) | A kind of walnut protein coffee-whitening powder and preparation method thereof | |
| CN107674905A (en) | Spirulina bioactive peptide, composition and preparation method | |
| CN1377590A (en) | Method for producing soybean protein hydrolyate and use | |
| CN107156747A (en) | A kind of cordyceps sinensis walnut peptide nutrient powder and preparation method thereof | |
| CN107653289A (en) | A kind of industrialized production kardiseed active peptide and preparation method | |
| CN1101471C (en) | Process for preparing active peptide of soybean protein | |
| WO2021082311A1 (en) | Pea peptide having supplementary blood glucose reducing function and preparation method therefor | |
| CN104872291A (en) | Preparation method of fermentation type cordyceps militaris soymilk beverage | |
| CN107760748A (en) | A kind of industrialized production walnut active peptide and preparation method | |
| CN101878815A (en) | Production method of donkey-hide gelatin small peptide Chinese date yoghourt | |
| CN115413712B (en) | Oat milk and preparation method thereof | |
| CN107760746A (en) | A kind of industrialized production almond active peptide and preparation method | |
| CN107760747A (en) | A kind of industrialized production earthworm protein active peptide and preparation method | |
| CN109527163A (en) | A kind of walnut polypeptide coffee and preparation method thereof | |
| CN106889297A (en) | A kind of production technology of soy peptide powder | |
| CN112890208B (en) | Composite plant peptide powder, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180306 |