CN107737262A - A kind of health-oriented products with auxiliary hyperglycemic effect - Google Patents
A kind of health-oriented products with auxiliary hyperglycemic effect Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicinal effective-part composition for treating diabetes conditions, belong to the field of Chinese medicines.Said composition is formed according to a certain ratio by extracting extraction root of kudzu vine Stigma Maydis total flavones in ramulus mori manyprickle acanthopanax general polysaccharide and root of kudzu vine corn stigma from ramulus mori wilsonii, adds pharmacy customary adjuvant, various formulations are made, and can be clinically used for treating diabetes conditions.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof with treatment diabetes conditions effect, specifically
It is Chinese medicine composition extracted from ramulus mori, wilsonii, the root of kudzu vine, corn stigma and preparation method thereof, belongs to the field of Chinese medicines.
Background technology
Diabetes are one group of metabolic diseases characterized by hyperglycaemia.Hyperglycaemia be due to then defect of insulin secretion or
Its biological agent is damaged, or both have concurrently and cause.Long-standing hyperglycaemia during diabetes, cause various tissues, particularly eye,
Kidney, heart, blood vessel, the chronic lesion of nerve, dysfunction.Long-term blood glucose increases, big blood vessel, capilary be damaged and jeopardize the heart,
Brain, kidney, peripheral nerve, eyes, foot etc., are counted according to the World Health Organization, and diabetic complication is up to kind more than 100, be at present
Know a kind of most disease of complication.Caused by Diabetes Death person has more than half to be cardiovascular and cerebrovascular, caused by 10% is nephropathy.
Because the patient of diabetes amputation is 10~20 times of non-diabetic.Clinical data shows 10 years or so after onset diabetes, will have
A kind of complication can at least occur for 30%~40% patient, and complication, once producing, drug therapy is difficult to reverse, therefore by force
Tune prevents diabetes as early as possible.It has been shown that, ended to 2015 according to IDF latest datas, in global range, in every 11 adults just
There is 1 people with diabetes (amounting to 4.15 hundred million)；There are 3.18 hundred million pre-diabetics, diabetes prevalence is up to 8.8%, glycosuria
The illness rate of sick early stage has 6.7%；If being not added with intervening, to the year two thousand forty, just there is 1 people to suffer from diabetes in every 10 adults
(6.42 hundred million), prediabetes crowd is up to 4.81 hundred million, therefore the treatment situation of diabetes allows of no optimist.
It is embodied in by the way that the advantage of western medical treatment diabetes is main in the diagnosis and effect of clinic, diabetes disease is examined
It is disconnected.By the medical history of patient, clinical manifestation and the inspection in every laboratory, the diagnosis, by stages of disease can be specified, including is
It is no to have the complication such as heart and brain kidney.By diagnosis, development trend and the prevention of diabetes disease are apparent that, its treatment and pre-
Anti- offer foundation.Antidiabetic drug effect is stronger.Perhaps potent antidiabetic drug is particularly developed in recent years, can effectively control blood glucose.To certain
A little impaired organs have reverse effect, can such as myocardial hypertrophy be mitigated or eliminated.It is rapid to the effect of diabetes disease acute disease.Western medicine hypoglycemic
The medicine such as drip-feed such as sodium nitroprussiate, phentolamine, effect are rapid.But western medicine therapy is in terms of diabetes are treated that there is also very
Big weak point, wherein a most important performance is exactly that side effect is larger, if any medicine can influence water and electrolyte metabolism,
Blood fat, metabolism of blood glucose are influenceed, some medicine long-term uses can also affect on sexual function etc..In addition, during hypoglycemic blood glucose fluctuation compared with
Greatly, early stage or senile diabetes are particularly, very sensitive to Western medicine antidiabetic drug, a medication blood glucose may be fallen too low, even
The orthostatic hypoglycemia of generation, reduces dosage or secondary, and blood glucose may raise quickly again.
" quenching one's thirst " sick category of the diabetes spp traditional Chinese medical science.This disease exists《The Yellow Emperor's Canon of Internal Medicine》In be referred to as " disappear pain ".According to pathogenesis
With the difference of clinical manifestation, still there are the different names such as " separate and disappear ", " lung disappears ", " in disappearing " in successive dynasties medicine writings.The traditional Chinese medical science thinks to cause to disappear
The principle pathological mechanism of thirsty disease is accumulated heat impairment of yin, fire excess from yin deficiency, consume lung, spleen (stomach)；Kidney is all dirty, and thermal burn lung yin, lung liquid is done
Dry up ', compress dereliction of duty, drinks more and polydipsia is more than；Heresy injures one's stomach the moon, exuberance of stomach fire, the kind drink of the paddy that disappears, wasting；Heat evil is impairment of the kidney, kidney yin
Virtual loss, vital essence are taken the photograph and missed a chance admittedly less than, precise and tiny not hide, and diuresis is on the point of, or pleasantly sweet, or such as fat.Though its beginning of this three is different, its
It is whole then same, lung, stomach, kidney yin liquid are finally damaged, and cause cost sick.The etiology and pathogenesis, dialectical of diabetes is being explored and summarized to the traditional Chinese medical science
Parting, the side's of sending medication, the treatment of complication etc., make encouraging progress.Many researchers also do to diabetes patient
The check and inspections such as pretherapy and post-treatment blood glucose, glucose in urine carbohydrate tolerance test.Lot of research shows, traditional Chinese medical herbal treatment diabetes, no
Only clinical efficacy is satisfied with, and is quite had one's own knack.
But because prepared slices of Chinese crude drugs active constituent content is low, dose is big, it has not been convenient to, flavour of a drug are bitter, so being not easy by patient
Receive, but the extraction Jing Guo modern science technique so that active constituent content is improved, and dosage is reduced, convenient to take, and good medicine
It is no longer bitter to the taste, add the crowd of receiving.
The content of the invention
It is an object of the present invention to provide a kind of effective ingredient in Chinese combination with treatment diabetes conditions effect
Thing；
It is another object of the present invention to provide the preparation method of above-mentioned Chinese medicine composition and purposes.
The first object of the present invention：Using ramulus mori manyprickle acanthopanax general polysaccharide, root of kudzu vine Stigma Maydis total flavones as raw material, appropriate medicine is added
With auxiliary material, related formulation is made.
The composition and ratio of the bulk drug of the composition of medicine of the present invention are：The parts by weight of ramulus mori manyprickle acanthopanax general polysaccharide 7, the root of kudzu vine
The parts by weight of Stigma Maydis total flavones 4.
Total starches are not less than the 60% of extract in above-mentioned ramulus mori manyprickle acanthopanax general polysaccharide active component, and root of kudzu vine corn stigma is always yellow
The content of Puerarin is 4.8%~6.72% in ketone active component.
The pharmaceutical composition can add corresponding auxiliary material, and appointing in tablet, granule, hard capsule, soft capsule is made
It is a kind of.Then can add other have treatment active components of diabetes conditions medicine, active ingredient, synthetic or
Person's semi-synthetic, then adds corresponding auxiliary material, and any of tablet, granule, hard capsule, soft capsule is made.
The preparation method of Chinese medicine composition of the present invention comprises the following steps：
The preparation of ramulus mori manyprickle acanthopanax general polysaccharide：Take the parts by weight of ramulus mori 2 and the parts by weight medicinal material of wilsonii 3, dosing material weight 10
Amount water extracts 3 times, each 2h again, and filtration, filtrate merges, and is concentrated under reduced pressure into 1g crude drugs/mL, adds ethanol to alcohol content 50%, cold
Hide and stand 12h, filtration, filtrate is continued plus ethanol is to alcohol content 80%, is collected precipitation, repeatedly purifying precipitation, is added 15 times of amount water to make
Fully dissolving, 2% tannic acid is added, mixing, 12h is stood, centrifugation, takes supernatant to add 3% activated carbon, in 75 DEG C of insulation decolourings 3
It is secondary, 0.5h/ times, filtration, filtrate decompression concentration, dry, obtain ramulus mori manyprickle acanthopanax general polysaccharide；Wherein polyoses content is not less than extract
60%；
The preparation of root of kudzu vine Stigma Maydis total flavones：The parts by weight of the root of kudzu vine 4 and the parts by weight of corn stigma 3 are taken, add 15 times of 70% ethanol of amount
Refluxing extraction 2 times, each 1.5h, filtration, filtrate merge, ethanol are recovered under reduced pressure, is diluted with water concentrate, according to amount of resin:Medicine
Material amount is 1:1, by macroporous resin column, first with 6 times of amount deionized water elutions, then with 8 times of 70% ethanol elutions of amount, collects elution
Liquid, ethanol is recovered under reduced pressure and concentrates, dry, obtain root of kudzu vine Stigma Maydis total flavones；Wherein the content of Puerarin be 4.8%~
6.72%；
Weight proportion according to each active component takes above-mentioned two parts of active components, is not added with auxiliary material or addition pharmacy is routinely auxiliary
Material, is made pharmaceutically acceptable formulation.
The content assaying method of ramulus mori manyprickle acanthopanax general polysaccharide is：Precision weighs 105 DEG C of dryings to the glucose control of constant weight
Product 10mg, it is placed in 100mL volumetric flasks, after adding distilled water dissolving completely, is settled to scale, shakes up, obtaining concentration of glucose is
100ug/mL standard liquid.Respectively it is accurate inhale glucose standards solution 0.0mL, 0.2mL, 0.4mL, 0.6mL, 0.8mL,
1.0mL, 1.2mL, 1.4mL add distilled water to mend to 2.0mL in 10mL tool plug test tubes, are separately added into 6% phenol solution 1.0mL
Mix, rapidly join the 5.0mL concentrated sulfuric acids, mix, after 60 DEG C of insulation reaction 15min, stop reacting with ice-water bath 15min, with No. 0
Manage as reference, absorbance is surveyed at 490nm, using the amount of glucose as abscissa, absorbance is ordinate, draws standard curve.
Ramulus mori manyprickle acanthopanax general polysaccharide active component 15mg is taken, is placed in 100mL volumetric flasks, after adding distilled water dissolving completely, is settled to quarter
Degree.1mL test solutions are drawn in 10mL tool plug test tubes, adds distilled water to mend to 2.0mL, is separately added into 6% phenol solution 1.0mL
Mix, rapidly join the 5.0mL concentrated sulfuric acids, mix, after 60 DEG C of insulation reaction 15min, stop reacting with ice-water bath 15min, measure
Absorbance, substitute into standard curve, calculate and produce.
Root of kudzu vine corn stigma puerarin content assay method：Precision, which weighs, to be dried under reduced pressure at 120 DEG C to the Puerarin pair of constant weight
According to product about 10mg, put in 100mL volumetric flasks, add 70% ethanol 70mL, putting water-bath low-grade fever makes dissolving, lets cool, and adds 70% ethanol extremely
Scale, shake up, produce.Precision draws reference substance solution 1.0,2.0,3.0,4.0,5.0mL, is respectively placed in 10mL measuring bottles, point
Not plus water 4.0,3.0,2.0,1.0,0mL, add 5% sodium nitrite solution 0.3mL, shake up, place 6min, add 10% aluminum nitrate molten
Liquid 0.3mL, shakes up, and places 6min, adds 4% sodium hydroxide 4mL, add water scale, shake up, and places 12min；Blank is obtained with legal system
Solution.Absorbance is determined at 250nm.Using trap as ordinate, concentration is abscissa, draws standard curve.Take test sample
100mg, into 100mL volumetric flasks, 70% ethanol is added to shake up, filter to scale.Precision measures filtrate 3mL, adds 2mL water, adds
5% sodium nitrite solution 0.3mL, shakes up, and places 6min, adds 10% aluminum nitrate solution 0.3mL, shake up, and places 6min, adds 4%
Sodium hydroxide 4mL, adds water scale, shakes up, and places 12min.Absorbance is determined in 250nm, and according to calibration curve equation meter
Calculate content.
After the contrast of inventor is preferred, it is determined that the best weight ratio of above-mentioned active component is：Ramulus mori wilsonii
The parts by weight of total starches 7, the parts by weight of root of kudzu vine Stigma Maydis total flavones 4.Proportionally take above-mentioned ramulus mori manyprickle acanthopanax general polysaccharide and the root of kudzu vine beautiful
Rice palpus general flavone, it is not added with auxiliary material or adds pharmacy customary adjuvant, pharmaceutically acceptable formulation is made.
The formulation that pharmaceutical composition of the present invention can be made into is tablet, granule, hard capsule, soft capsule.
Foregoing pharmacy customary adjuvant includes but is not limited to adhesive, such as cellulose derivative, alginates, gelatin
And polyvinylpyrrolidone；Diluent, starch, Icing Sugar, dextrin, lactose, microcrystalline cellulose, mannitol；Lubricant, such as talcum
Powder, magnesium stearate, superfine silica gel powder and polyethylene glycol；Disintegrant, such as carboxyrnethyl starch sodium, low-substituted hydroxypropyl methylcellulose, crosslinking carboxylic
Methylcellulose；Sorbefacient, such as quaternary ammonium compound；Surfactant, such as lauryl sodium sulfate；Excipient, as lactose,
Starch, sucrose, dextrin, calcium carbonate, aluminum sulfate, magnesia, magnesium stearate, sodium acid carbonate, glycerine, propane diols, polyethylene glycol, mountain
Pears sugar alcohol, lanolin, vaseline, microcrystalline cellulose, honeybee are cured, wood is cured, atoleine, resin, advanced wax, pressure sensitive adhesive, semi-synthetic
Fatty acid ester etc..
Active component composition of the present invention is used to treat diabetes conditions.
Beneficial effect
The present invention is using Traditional Chinese medical theory as guiding theory, in combination with correlative study achievement both domestic and external, in treatment sugar
Urinate the aspect significant effect of disease.The root of kudzu vine is monarch drug in a prescription in side, and the root of kudzu vine is sweet, pungent, cool in nature.Attach to the lung and stomach meridians.Brought down a fever with expelling pathogenic factors from muscles and skin, thoroughly
Rash, promote the production of body fluid to quench thirst, the effect of Shengyang Zhixie.Being generated heat for exterior syndrome, stiff nape and back, measles without adequate eruption, pyreticosis is thirsty, and the deficiency of Yin is quenched one's thirst,
Heat is purged heat dysentery, splenasthenic diarrhea, therefore is monarch drug in a prescription.Ramulus mori is ministerial drug, ramulus mori slight bitter, is put down.Return liver warp.With wind-damp dispelling, sharp joint, OK
The effect of aqueous vapor.For controlling wind-cold-dampness arthralgia, spasm of limbs, beriberi edema, human body pruritus.Ramulus mori has with root of kudzu vine collocation preferably uses life
Tianjin is quenched the thirst, dredging collateral, sharp water clearing damp the effect of, therefore be ministerial drug.Wilsonii is adjutant, and wilsonii acrid flavour, bitter, micro-sweet is warm-natured, Return liver
Through, kidney channel, there is wind-damp dispelling, filling liver kidney, strengthening the bones and muscles, invigorate blood circulation arteries and veins the effect of, wilsonii belongs to Qi-tonifying drug, has what qi-restoratives was helped the weak
Effect, it can prevent or treat the disease having a delicate constitution, therefore be adjutant.Corn stigma is sweet, light, mild-natured, returns bladder, liver, gallbladder channel,
There is the effect of inducing diuresis to remove edema, clearing liver cholagogic.With diuresis, suitable for diabetic, and corn stigma is mild-natured, can reconcile
All medicines, therefore to make medicine.Each medicine complements each other in we, indispensable, by traditional Chinese medical science monarch, minister, helps, makes theoretical reasonable formula, altogether
Play nourishing the liver and kidney, nourishing kidney yin, qi and activate blood circulation, the work(of stagnation resolvation profit water.
Product composition of the present invention is clear and definite, and curative effect is stable, and dose is small, safe, not only conforms with Chinese Traditional Medicine treatment
The principles of formulating prescriptions of diabetes, and meet the demand for development of the modernization of Chinese medicine, to promoting medicinal industry from copying to autonomous innovation
Change and to promoting China's internationalization of tcm significant.Through consulting pertinent literature and patent, do not retrieve with the root of kudzu vine,
Ramulus mori, wilsonii, the active component of corn stigma produce and process as raw material and Suitable pharmaceutical preparations controlling for diabetes are made
Treatment or the report of auxiliary treatment.
For the pharmacological action of the further checking present invention, animal pharmacodynamic experiment is carried out with capsule of the present invention.
Content of the test：The hypoglycemic effect of diabetic model rats model is studied.
1 reagent, sample and animal
1.1 reagent
Streptozotocin, blood sugar detection kit.
1.2 test sample
Sample 1：(capsule of the present invention)
Capsule prepared by embodiment 1.
Sample 2：
Ramulus mori 60g, wilsonii 50g are taken, root of kudzu vine 75g, corn stigma 55g, adds 10 times of amount decoctings to boil 2 hours, filters, the dregs of a decoction are again
Add 8 times of amount decoctings to boil 1.5 hours, filter, merging filtrate, be concentrated under reduced pressure, be dried under reduced pressure, crush, add starch to 150g, load
No. 1 capsule, per the tolerant 0.3g of intragranular, capsule is made.
Sample 3：
Ramulus mori manyprickle acanthopanax general polysaccharide 50g, root of kudzu vine Stigma Maydis total flavones 80g, starch is added to 150g, loads No. 1 capsule, often
The tolerant 0.3g of intragranular, is made capsule.
Sample 4：
Ramulus mori manyprickle acanthopanax general polysaccharide 150g is taken, adds starch to load No. 1 capsule to 200g, per the tolerant 0.3g of intragranular, glue is made
Wafer.
Sample 5：
Root of kudzu vine Stigma Maydis total flavones 150g is taken, adds starch to load No. 1 capsule to 200g, per the tolerant 0.3g of intragranular, glue is made
Wafer.
Before administration, above-mentioned corresponding preparations are taken, it is finely ground, required drug concentration is configured to using pure water.
1.3 experimental animal
Wistar rats, male, body weight (200 ± 20g) are provided by Shandong Traditional Chinese Medicine University's experimental animal center.
2 experimental methods
After the preparation rat adaptability of hypertensive rat model is fed 1 week, high-sugar-fat-diet is given, after feeding 4 weeks, is prohibited
12h is eaten, (Streptozotocin is dissolved in 0.1mol/L citrate buffer solutions, pH=with 35mg/kg dosage intraperitoneal injection Streptozotocin
4.5).After continuing high-sugar-fat-diet nursing 1 week, fasting 12h, blood 1mL is taken from rat angular vein using glass capillary, led to
Fasting blood-glucose (FBG) value of determination of glucose oxidase rat is crossed, FBG >=7.8mmol/L is considered as diabetes rat model system
Standby success.
Packet randomly selects diabetic model rats 60 with administration, 6 groups is equally divided into, wherein 5 sample sets fill daily
The sample of stomach 50mg/kg dosage, model group gavage same dose distilled water, continue gavage 4 weeks, feed high-sugar-fat-diet,
Rat fasting blood-glucose value is determined after experiment terminates.
Influence of the sample of table 1 to hypertensive rat blood pressure
Five groups of result of the tests are substantially 4,5 groups of 1 ＞ samples of sample, 2 ＞ samples, 3 ＞ samples.Combination matching selected by explanation is most
Excellent.
Embodiment
Carry out the technical scheme that the present invention is furture elucidated below by way of specific embodiment, but claimed by the present patent application
Content not only that.Dosage in actual production is not limited only to the drug dose in example, can use ton, kilogram, gram etc.
Measurement unit, but the consumption proportion of each material medicine is also in accordance with the bright technical scheme of we.
Embodiment 1：
Prescription：Ramulus mori manyprickle acanthopanax general polysaccharide 70g, root of kudzu vine Stigma Maydis total flavones 40g
Preparation method：The preparation of ramulus mori manyprickle acanthopanax general polysaccharide：The parts by weight of ramulus mori 2 and the parts by weight medicinal material of wilsonii 3 are taken, add medicinal material
10 times of amount water of weight extract 3 times, each 2h, and filtration, filtrate merges, and is concentrated under reduced pressure into 1g crude drugs/mL, adds ethanol to alcohol content
50%, refrigeration stands 12h, filtration, and filtrate is continued plus ethanol is to alcohol content 80%, collects precipitation, purifying precipitation, adds 15 times repeatedly
Amount water makes fully to dissolve, and adds 2% tannic acid, mixes, and stands 12h, centrifugation, takes supernatant to add 3% activated carbon, in 75 DEG C of insulations
Decolourize 3 times, 0.5h/ times, filtration, filtrate decompression concentration, dry, obtain ramulus mori manyprickle acanthopanax general polysaccharide；Wherein polyoses content is not less than
The 60% of extract；
The preparation of root of kudzu vine Stigma Maydis total flavones：The parts by weight of the root of kudzu vine 4 and the parts by weight of corn stigma 3 are taken, add 15 times of 70% ethanol of amount
Refluxing extraction 2 times, each 1.5h, filtration, filtrate merge, ethanol are recovered under reduced pressure, is diluted with water concentrate, according to amount of resin:Medicine
Material amount is 1:1, by macroporous resin column, first with 6 times of amount deionized water elutions, then with 8 times of 70% ethanol elutions of amount, collects elution
Liquid, ethanol is recovered under reduced pressure and concentrates, dry, obtain root of kudzu vine Stigma Maydis total flavones；Wherein puerarin content is 4.8%~6.72%；
Above-mentioned each active component is taken, is crushed, mixing, starch is added to 150g, mixes, granulation, load capsule, produce.
Embodiment 2：
Prescription：Ramulus mori manyprickle acanthopanax general polysaccharide 70g, root of kudzu vine Stigma Maydis total flavones 40g；
Preparation method：The preparation of ramulus mori manyprickle acanthopanax general polysaccharide：The parts by weight of ramulus mori 2 and the parts by weight medicinal material of wilsonii 3 are taken, add 10 times of amounts
Water extracts 3 times, each 2h, and filtration, filtrate merges, and is concentrated under reduced pressure into 1g crude drugs/mL, adds ethanol quiet to alcohol content 50%, refrigeration
12h is put, is filtered, filtrate is continued plus ethanol is to alcohol content 80%, is collected precipitation, repeatedly purifying precipitation, is added 15 times of amount water to make fully
Dissolving, 2% tannic acid is added, mixed, stood 12h, centrifugation, take supernatant to add 3% activated carbon, decolourized 3 times in 75 DEG C of insulations,
0.5h/ times, filtration, filtrate decompression concentration, dry, obtain ramulus mori manyprickle acanthopanax general polysaccharide；Wherein polyoses content is not less than extract
60%；
The preparation of root of kudzu vine Stigma Maydis total flavones：The parts by weight of the root of kudzu vine 4 and the parts by weight of corn stigma 3 are taken, add 15 times of 70% ethanol of amount
Refluxing extraction 2 times, each 1.5h, filtration, filtrate merge, ethanol are recovered under reduced pressure, is diluted with water concentrate, according to amount of resin:Medicine
Material amount is 1:1, by macroporous resin column, first with 6 times of amount deionized water elutions, then with 8 times of 70% ethanol elutions of amount, collects elution
Liquid, ethanol is recovered under reduced pressure and concentrates, dry, obtain root of kudzu vine Stigma Maydis total flavones；Wherein puerarin content is 4.8%~6.72%；
Above-mentioned each active component is taken, is crushed, mixing, adds hydroxypropyl methylcellulose 20g, microcrystalline cellulose 10g, superfine silica gel powder
5g adds lactose to be mixed, granulation is tabletted, produces to 600g again.
Embodiment 3：
Prescription：Ramulus mori manyprickle acanthopanax general polysaccharide 140g, root of kudzu vine Stigma Maydis total flavones 80g；
Preparation method：The preparation of ramulus mori manyprickle acanthopanax general polysaccharide：The parts by weight of ramulus mori 2 and the parts by weight medicinal material of wilsonii 3 are taken, add medicinal material
10 times of amount water of weight extract 3 times, each 2h, and filtration, filtrate merges, and is concentrated under reduced pressure into 1g crude drugs/mL, adds ethanol to alcohol content
50%, refrigeration stands 12h, filtration, and filtrate is continued plus ethanol is to alcohol content 80%, collects precipitation, purifying precipitation, adds 15 times repeatedly
Amount water makes fully to dissolve, and adds 2% tannic acid, mixes, and stands 12h, centrifugation, takes supernatant to add 3% activated carbon, in 75 DEG C of insulations
Decolourize 3 times, 0.5h/ times, filtration, filtrate decompression concentration, dry, obtain ramulus mori manyprickle acanthopanax general polysaccharide；Wherein polyoses content is not less than
The 60% of extract；
The preparation of root of kudzu vine Stigma Maydis total flavones：Take the parts by weight of the root of kudzu vine 4 and the parts by weight of corn stigma 3,15 times of amounts of dosing material weight
70% alcohol reflux extracts 2 times, each 1.5h, and filtration, filtrate merges, and ethanol is recovered under reduced pressure, is diluted with water concentrate, according to tree
Fat amount:Medicinal material amount is 1:1, by macroporous resin column, first with 6 times of amount deionized water elutions, then with 8 times of 70% ethanol elutions of amount, receives
Collect eluent, ethanol is recovered under reduced pressure and concentrates, dry, obtain root of kudzu vine Stigma Maydis total flavones；Wherein puerarin content be 4.8%~
6.72%；
Above-mentioned each active component is taken, adds 10% starch, 90% alcohol granulation, produces.
Embodiment 4：
Prescription：Ramulus mori manyprickle acanthopanax general polysaccharide 140g, root of kudzu vine Stigma Maydis total flavones 80g
Preparation method：Take ramulus mori manyprickle acanthopanax general polysaccharide, astragalus root total saponin, salvianolic acid, root of kudzu vine Stigma Maydis total flavones addition polymerization second
Glycol 400 mixes to total amount 300g, suppresses soft capsule, produce.
Embodiment 5：
Prescription：Ramulus mori manyprickle acanthopanax general polysaccharide 3.5kg, root of kudzu vine Stigma Maydis total flavones 2kg；
Preparation method：Above-mentioned each active component is taken, adds 10% starch, 90% alcohol granulation, produces.
Embodiment 6：
120 diabetics of made Granules in Treating of Example 5.Therapeutic scheme：Orally, once a bag, 3 times a day,
4 weeks are a course for the treatment of.As a result：Take a turn for the better 46, effective 63, invalid 11, total effective rate 90.8%.
Claims (4)
1. a kind of health-oriented products with auxiliary hyperglycemic effect, it is characterised in that be by extracting ramulus mori thorn from ramulus mori wilsonii
Root of kudzu vine Stigma Maydis total flavones composition is extracted in slender acanthopanax total starches and root of kudzu vine corn stigma, wherein, the weight proportion of each active component
For：
The parts by weight of ramulus mori manyprickle acanthopanax general polysaccharide 2~15
The parts by weight of root of kudzu vine Stigma Maydis total flavones 3~10.
2. the health-oriented products of auxiliary hyperglycemic effect as claimed in claim 1, it is characterised in that the weight of each active component is matched somebody with somebody
Than for：The parts by weight of ramulus mori manyprickle acanthopanax general polysaccharide 7, the parts by weight of root of kudzu vine Stigma Maydis total flavones 4.
3. the health-oriented products of auxiliary hyperglycemic as claimed in claim 1 or 2 effect, it is characterised in that its preparation method include with
Lower step：
The preparation of ramulus mori manyprickle acanthopanax general polysaccharide：Take the parts by weight of ramulus mori 2 and the parts by weight medicinal material of wilsonii 3,8~20 times of dosing material weight
Measure water to extract 1~3 time, each 2h, filtration, filtrate merges, and is concentrated under reduced pressure into 1g crude drugs/mL, adds ethanol to alcohol content 50%, cold
Hide and stand 12h, filtration, filtrate is continued plus ethanol to alcohol content 80%, refrigeration stands 12h, collects precipitation, purifying precipitation, adds repeatedly
5~20 times of amount water make fully to dissolve, and add 0.5%~5% tannic acid, mix, and stand 12h, centrifugation, take supernatant to add 0.5%~5%
Activated carbon, decolourized 3 times in 80 DEG C of insulations, 0.5h/ times, filtration, filtrate decompression concentration, dry, obtain ramulus mori manyprickle acanthopanax general polysaccharide；Its
Middle polyoses content must not be less than the 60% of extract；
The preparation of root of kudzu vine Stigma Maydis total flavones：The parts by weight of the root of kudzu vine 4 and the parts by weight of corn stigma 3 are taken, 8-15 times of dosing material weight measures 60%
~80% alcohol reflux extracts 2 times, each 1.5h, and filtration, filtrate merges, and ethanol is recovered under reduced pressure, is diluted with water concentrate, according to
Amount of resin:Medicinal material amount is 1:1 by macroporous resin column, first with 6~8 times of amount deionized water elutions, then with 8~10 times of 70% second of amount
Alcohol elutes, and collects eluent, ethanol is recovered under reduced pressure and concentrates, and dries, obtains root of kudzu vine Stigma Maydis total flavones；The wherein content of Puerarin
For 4.8%~6.72%；
Weight proportion according to each active component takes above-mentioned two parts of active components, it is characterised in that is added without or adds and pharmaceutically may be used
The auxiliary material of receiving, pill, tablet, granule, hard capsule, mixture, pill, soft capsule, effervescent tablet, injection is made
Or any of powder-injection.
4. the health-oriented products of the auxiliary hyperglycemic effect described in claim 1 or 2 are being prepared for treating in diabetes medicament
Using.
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Application publication date: 20180227 |