CN107737145A - A kind of preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract - Google Patents
A kind of preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract Download PDFInfo
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- CN107737145A CN107737145A CN201711000423.3A CN201711000423A CN107737145A CN 107737145 A CN107737145 A CN 107737145A CN 201711000423 A CN201711000423 A CN 201711000423A CN 107737145 A CN107737145 A CN 107737145A
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses a kind of preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract, comprise the following steps：(1) Feedstock treating：Ginseng/American ginseng medicine is carried out disinfection, washing and drying treatment, obtain net system ginseng/American Ginseng；(2) crush：Net system ginseng/ginseng powder is broken into nanometer powder, obtains ginseng/American Ginseng nanometer powder；(3) it is inoculated with：Added in ginseng/American Ginseng nanometer powder after nitrogen source, carbon source and water stir and be inoculated with probiotics and saccharomycete, obtain fermentation materials；(4) ferment：Fermentation materials ferment more than 1 day at 45 55 DEG C；(5) filter：After the completion of fermentation, the filtered processing of fermenting mixture of gained, ginseng/American Ginseng enzyme liquid, i.e. ginseng/American ginseng extract are obtained.Method provided by the invention is more effectively discharged the active principle contained in ginseng/American Ginseng using the collective effect of probiotics and saccharomycete, and compared with traditional extraction process, active constituent content significantly improves in extract.
Description
Technical field
The present invention relates to ginseng/American Ginseng extracting method, more particularly to a kind of ginseng/American Ginseng nanometer decomposing and fermenting extraction
The preparation method of thing.
Background technology
Ginseng is the perennial root grass for belonging to Araliaceae Panax in Plant Taxonomy, on earth known kind
About 11 kinds, representative kind has Korean ginseng, Changbai Mountain ginseng, American Ginseng.Korean ginseng is mainly grown in Far East Asia (Korea Spro
State, Northeast China be northern, Russian some areas), drug effect is outstanding.Changbai Mountain ginseng is mainly grown in the band of northeast Changbai Mountain one,
American Ginseng is mainly in the U.S., Canada's area growth and cultivation.
With delivering for ginseng main component and pharmacological action correlative study, attracted tremendous attention as natural health food,
Its demand also gradually increases, and is further speeded up because of the raising of economy and living standards of the people, is being widely used as popular type
Natural health food.Sweet, the slight bitter of ginseng, tepor, have reinforce vital energy, veins takes off admittedly, reinforce the spleen to benefit the lung, blood-nourishing of promoting the production of body fluid, peace
The effect of refreshing intelligence development, while its complex chemical composition, bioactivity is extensive, and pharmacological action is unique.As modern times separation is analyzed
The development of technology and medical sci-tech, the research of the chemical composition of ginseng make some progress, and it mainly contains ginsenoside
Class, polysaccharide, volatile oil, aliphatic acid and some trace elements.Modern pharmacology research proves that ginseng has antitumor, tune
Nervous system is saved, improves cardiovascular system, anti-oxidant, hypoglycemic and raising immunologic function etc..
The content of the invention
It is an object of the invention to provide a kind of preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract, this method
The active principle contained in ginseng/American Ginseng is more effectively discharged using the collective effect of probiotics and saccharomycete, with
Traditional extraction process is compared, and active constituent content significantly improves in extract.
The purpose of the present invention is achieved through the following technical solutions：A kind of ginseng/American Ginseng nanometer decomposing and fermenting carries
The preparation method of thing is taken, is comprised the following steps：
(1) Feedstock treating：Ginseng/American ginseng medicine is carried out disinfection, washing and drying treatment, obtain net ginseng/the West
Ginseng；
(2) crush：Net ginseng/ginseng powder is broken into nanometer powder, obtains ginseng/American Ginseng nanometer powder；
(3) it is inoculated with：Add nitrogen source in ginseng/American Ginseng nanometer powder, probiotics is inoculated with after carbon source and water stir and
Saccharomycete, obtain fermentation materials；
(4) ferment：Fermentation materials ferment more than 1 day at 45-55 DEG C；
(5) filter：After the completion of fermentation, the filtered processing of fermenting mixture of gained, ginseng/American Ginseng enzyme liquid is obtained,
That is ginseng/American ginseng extract.
In the step (1), ginseng/American ginseng medicine is carried out disinfection processing using Ozone Water, and the drying is then adopted
With air-dried or less than 50 DEG C low temperature dryings.
In the step (2), net ginseng/ginseng powder is broken into by nanometer powder using substep grinding mode, is used as this hair
Bright one embodiment, is carried out in three steps crushing in the present invention, and net ginseng/ginseng powder is broken into the big of 2-5mm by the first step
Net ginseng/american ginseng granules are crushed to the little particle that particle diameter is 0.3mm-15 μm by particle, second step, and the 3rd step continues net people
Ginseng/American Ginseng little particle is crushed to nano particle, produces ginseng/American Ginseng nanometer powder.
In the step (3), the inoculum density of probiotics is 1%~10%, and saccharomycete inoculum density is 1%~10%.
The probiotics includes but is not limited to lactobacillus and Bifidobacterium.The lactobacillus includes but is not limited to lactobacillus acidophilus, cheese
Lactobacillus.The Bifidobacterium includes but is not limited to bifidobacterium longum, bifidobacterium breve, avette Bifidobacterium, thermophilic bifid bar
Bacterium.Described saccharomycete is Ascomycota Blastocystis.
In the step (3), per in 1kg ginsengs/American Ginseng nanometer powder, nitrogen source addition is 1-5 parts, carbon source and water
95-99 parts.The nitrogen source includes but is not limited to soybean protein, and the carbon source includes but is not limited to granulated sugar.The granulated sugar and water system
It is added to again in ginseng/American Ginseng ultra-micro powder after into the sugar solubility that concentration is 25-50%, and by the water of the fermentation materials
Sub-control system is within 10%.
In the step (4), fermentation materials fermentation time is preferably 10-40 days.
Further, present invention additionally comprises milling step, i.e. step (6)：Ginseng/American Ginseng enzyme liquid and excipient mixing
Uniformly, ginseng/American Ginseng ferment powder is obtained after freeze-drying.
The present invention has the advantages that compared with prior art：
The present invention uses microbial fermentation technology, using probiotics and the collective effect of saccharomycete, in ginseng/American Ginseng
The active principle contained more effectively discharges, as shown in Figure 2-5, compared with traditional extractive technique, ginseng/American Ginseng
Active ingredient, ginsenoside Rb1, ginsenoside Rb2, total saposins and polyoses content are higher, and can more play ginseng/American Ginseng has
The effect of strengthen immunity.
Brief description of the drawings
Fig. 1 is the flow chart of the preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract of the present invention.
Fig. 2 is 1-4 of embodiment of the present invention American Ginseng enzyme liquid compared with the polyoses content of comparative example.
Fig. 3 is 1-4 of embodiment of the present invention American Ginseng enzyme liquid compared with the total saponin content of comparative example.
Fig. 4 be 1-4 of the embodiment of the present invention American Ginseng enzyme liquid and comparative example ginsenoside Rb1 comparision contents.
Fig. 5 be 1-4 of the embodiment of the present invention American Ginseng enzyme liquid and comparative example ginsenoside Rb2 comparision contents.
Embodiment
It is the specific embodiment of the present invention below, technical scheme is described further, but the present invention
Protection domain is not limited to these embodiments, every to be included in this hair without departing substantially from the change of present inventive concept or equivalent substitute product
Within bright protection domain.
Probiotics that the present invention uses and saccharomycete be respectively obtain preservation lactobacillus (Lactobacillus sp,
Bacterial strain preserving number：CICC 10481) and saccharomyces cerevisiae (Sacchar omyces cerevisiae, culture presevation number：ATCC
204508), above probiotics and saccharomycete use after activation culture.
Embodiment 1
(1) more than 5 years plantation American ginseng medicine 1000g are chosen, are carried out disinfection processing using Ozone Water, low temperature drying (50
Below DEG C) or natural air drying, obtain net system American Ginseng.
(2) net system American Ginseng is put into pulverizer, is crushed to 40 μm of particles to 75 μm of sizes for the first time, for the first time
Crushed material puts into ACM pulverizers, after being crushed to 12 μm of particles to 22 μm of sizes for the second time, second of crushed material input coating
The dry type or wet ball mill of polyurethane, the nano particle of 500nm sizes is crushed to for the third time, obtain fermentation American Ginseng nanometer
Powder.
(3) 110g soybean proteins and 10kg 25% aqueous solution of granulated sugar are added in 1000g American Ginseng nanometer powders.Profit
Nutrient solution dissolved oxygen in fermentation tank is removed with nitrogen, sterilized at 90 DEG C.Then, lactobacillus and ferment are inoculated with each 5% concentration
Female bacterium, obtains fermentation materials.
(4) fermentation materials are cultivated 10 days under the conditions of 37 DEG C.
(5) after the completion of cultivating, 16 mesh screens are carried out, obtain American ginseng extract, i.e. American Ginseng enzyme liquid.
(6) American Ginseng enzyme liquid addition maltodextrin is appropriate, is spray-dried to obtain American Ginseng ferment powder.
Embodiment 2
(1) more than 5 years plantation American ginseng medicine 1000g are chosen, are carried out disinfection processing using Ozone Water, low temperature drying (50
Below DEG C) or natural air drying, obtain net system American Ginseng.
(2) net system American Ginseng is put into pulverizer, is crushed to 40 μm of particles to 75 μm of sizes for the first time, for the first time
Crushed material puts into ACM pulverizers, after being crushed to 12 μm of particles to 22 μm of sizes for the second time, second of crushed material input coating
The dry type or wet ball mill of polyurethane, the nano particle of 500nm sizes is crushed to for the third time, American Ginseng nanometer powder is made.
(3) 110g soybean proteins and 10kg 25% aqueous solution of granulated sugar, profit are added in 1000g American Ginseng nanometer powders
Nutrient solution dissolved oxygen in fermentation tank is removed with nitrogen, sterilized at 90 DEG C.Then, lactobacillus and ferment are inoculated with each 5% concentration
Female bacterium.
(4) fermentation materials are cultivated 20 days under the conditions of 37 DEG C.
(5) after the completion of cultivating, 16 mesh screens are carried out, obtain American ginseng extract, i.e. American Ginseng enzyme liquid.
(6) American Ginseng enzyme liquid addition maltodextrin is appropriate, is spray-dried to obtain American Ginseng ferment powder.
Embodiment 3
(1) more than 5 years plantation American ginseng medicine 1000g are chosen, are carried out disinfection processing using Ozone Water, low temperature drying (50
Below DEG C) or natural air drying, obtain net system American Ginseng.
(2) net system American Ginseng is put into pulverizer, is crushed to 40 μm of particles to 75 μm of sizes for the first time, for the first time
Crushed material puts into ACM pulverizers, after being crushed to 12 μm of particles to 22 μm of sizes for the second time, second of crushed material input coating
The dry type or wet ball mill of polyurethane, the nano particle of 500nm sizes is crushed to for the third time, fermentation American Ginseng nanometer is made
Powder.
(3) 110g soybean proteins and 10kg 25% sugar solubility are added in 1000g American Ginseng nanometer powders, is utilized
Nitrogen removes nutrient solution dissolved oxygen in fermentation tank, is sterilized at 90 DEG C.Then, lactobacillus and yeast are inoculated with each 5% concentration
Bacterium.
(4) fermentation materials are cultivated 30 days under the conditions of 37 DEG C.
(5) after the completion of cultivating, 16 mesh screens are carried out, obtain American ginseng extract, i.e. American Ginseng enzyme liquid.
(6) American Ginseng enzyme liquid addition maltodextrin is appropriate, is spray-dried to obtain American Ginseng ferment powder.
Embodiment 4
(1) more than 5 years plantation American ginseng medicine 1000g are chosen, are carried out disinfection processing using Ozone Water, low temperature drying (50
Below DEG C) or natural air drying, obtain net system American Ginseng.
(2) net system American Ginseng is put into pulverizer, is crushed to 40 μm of particles to 75 μm of sizes for the first time, for the first time
Crushed material puts into ACM pulverizers, after being crushed to 12 μm of particles to 22 μm of sizes for the second time, second of crushed material input coating
The dry type or wet ball mill of polyurethane, the nano particle of 500nm sizes is crushed to for the third time, fermentation American Ginseng nanometer is made
Powder.
(3) 110g soybean proteins and 10kg 25% sugar solubility are added in 1000g American Ginseng nanometer powders, is utilized
Nitrogen removes nutrient solution dissolved oxygen in fermentation tank, is sterilized at 90 DEG C.Then, lactobacillus and yeast are inoculated with each 5% concentration
Bacterium.
(4) fermentation materials are cultivated 40 days under the conditions of 37 DEG C.
(5) after the completion of cultivating, 16 mesh screens are carried out, obtain American ginseng extract, i.e. American Ginseng enzyme liquid.
(6) American Ginseng enzyme liquid addition maltodextrin is appropriate, is spray-dried to obtain American Ginseng ferment powder.
Experimental example 1
To periodically sampling carries out measurement of the polysaccharide content in the fermentation process of embodiment 1~4, measurement result is as shown in Figure 2.
It is prepared by glucose standards solution：Take and dry to the glucose 10mg of constant weight, it is accurately weighed, put in 100ml volumetric flasks
Constant volume after distilled water dissolving, it is 100 μ g/ml to be made into concentration, makees titer.
The drafting of standard curve：Take glucose standard 0.1ml, 0.25ml, 0.5ml, 1ml to put in test tube, add water to make into
1.0ml, 5% phenol solution 0.5ml is sequentially added, concentrated sulfuric acid 2.5ml, is shaken up immediately, it is determined at 488nm after 30min
Absorption value.
American Ginseng ferment powder in Example 1~4 and the American Ginseng ferment powder for not making nanosizing processing, it is accurate respectively to claim
Amount 0.1g is put into 10mL centrifuge tubes, adds pure water 10mL, and concussion is mixed, is put into 100 DEG C of hot baths with 50 turns/min
Concussion 2 hours, after letting cool, 3000 turns/min centrifugation 6min, after collecting supernatant and quantifying 10mL, centrifuge again and outwell supernatant
Liquid, 1mL dissolved in purified water sediments are added, it is standby.The American Ginseng ferment powder for not making nanosizing processing is by not making nanosizing
The American Ginseng of processing, which uses, to ferment and obtains with embodiment 1-4 identicals fermentation process.
Above-mentioned polysaccharide solution 0.5ml is taken, concentrated sulfuric acid 2.5ml is added, adds 5% phenol solution 0.5ml and slowly shake up,
Absorption value is determined under ultraviolet specrophotometer under conditions of 488nm.Measurement result is as shown in Figure 2.
As shown in Figure 2, American Ginseng carries out probiotics fermention 40 days after carrying out nanosizing processing, regular sampling analysis polysaccharide
Content is up to 2104mg/100g, and compared with without nanosizing treatment group, polyoses content substantially increases.
Experimental example 2
To in the fermentation process of embodiment 1~4 periodically sampling carry out ginsenoside Rb1 content, ginsenoside Rb2's content,
The measure of total saponin content, measurement result is as seen in figures 3-5.
The preparation of sample：Take American Ginseng ferment powder and do not make the American Ginseng ferment powder sample comminution of nanosizing processing, dry
40 mesh sieves are crossed afterwards, and each precision weighs 1g, adds 10ml water to soak 0.5 hour, decocts, and keeps micro-boiling 1h, filtering, and filter residue adds 6 times of water
Decoct, keep micro-boiling 1 hour, filtering, merging filtrate, filtrate is concentrated, with extracting n-butyl alcohol, each 10ml, totally 4 times, positive fourth
Alcohol layer distillation washing 2 times, discards water layer, and n-butanol is recovered under reduced pressure to doing, is dissolved with methanol and is settled to 1ml, standby.Sampling
After product solution is completely dissolved, filtered using filter (0.45 μm), then quantitatively divided with high performance liquid chromatograph (HPLC)
Ginsenoside composition is analysed.
The preparation of reference substance：1. take ginsenoside Rh 1 reference substance, ginsenoside Re's reference substance, ginsenoside R f controls
Product, ginsenoside Rg1's reference substance, ginsenoside Rg2's reference substance, ginseng sapoglycoside Rg 3 reference substance are accurately weighed, add methanol to be made
Per l mL respectively 0.lmg containing ginsenoside Rh 1, ginsenoside Re 0.4mg, ginsenoside Rf l m g, ginsenoside Rg1 0.2mg,
Ginsenoside Rg2 0.2mg, ginseng sapoglycoside Rg 3 0.2mg solution, are produced.
HPLC conditions are as follows：
Chromatographic column：MACHEREY-NAGEL GmbHNH2(250×4.6mm,5μm)
Chromatographic condition：Mobile phase (acetonitrile:Water:0.1% phosphoric acid solution=100:20:5)
Detector：ELSD
Flow velocity：1.2ml/min
Drift tube temperature：80℃
Flow rate of carrier gas：2.1mL/min.
2. take ginsenoside Rb1's reference substance, ginsenoside Rb2's reference substance, Ginsenoside Rc's reference substance, ginsenoside Rd couple
According to product, precision weighing, add methanol that every 1mL respectively 1.0mg containing ginsenoside Rb1, ginsenoside Rb2 1.0mg, ginsenoside is made
Rc0.5mg, ginsenoside Rd 1.0mg solution, are produced.
HPLC conditions are as follows：
Chromatographic column：MACHEREY-NAGEL GmbHNH2(250×4.6mm,5μm)
Chromatographic condition：Mobile phase (acetonitrile:Water:0.1% phosphoric acid solution=100:25:5)
Detector：ELSD
Flow velocity：1.2ml/min
Drift tube temperature：80℃
Flow rate of carrier gas：2.1mL/min.
Fig. 3, Fig. 4 and Fig. 5 show total saposins, ginsenoside Rb1 and ginsenoside during American Ginseng nanometer decomposing and fermenting
Rb2 contents substantially increase compared with untreated fish group.
Claims (9)
1. a kind of preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, comprise the following steps：
(1) Feedstock treating：Ginseng/American ginseng medicine is carried out disinfection, washing and drying treatment, obtain net system ginseng/American Ginseng；
(2) crush：Net system ginseng/ginseng powder is broken into nanometer powder, obtains ginseng/American Ginseng nanometer powder；
(3) it is inoculated with：Added in ginseng/American Ginseng nanometer powder after nitrogen source, carbon source and water stir and be inoculated with probiotics and yeast
Bacterium, obtain fermentation materials；
(4) ferment：Fermentation materials ferment more than 1 day at 45-55 DEG C；
(5) filter：After the completion of fermentation, the filtered processing of fermenting mixture of gained, ginseng/American Ginseng enzyme liquid, i.e. people is obtained
Ginseng/American ginseng extract.
2. the preparation method of ginseng according to claim 1/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, institute
State in step (1), ginseng/American ginseng medicine carried out disinfection processing using Ozone Water, and the drying then using air-drying or
Less than 50 DEG C of low temperature drying.
3. the preparation method of ginseng according to claim 1/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, institute
State in step (2), ginseng/ginseng powder is broken into by nanometer powder using substep grinding mode.
4. the preparation method of ginseng according to claim 3/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, institute
State step (2) use and be carried out in three steps crushing, net ginseng/ginseng powder is broken into 2-5mm bulky grain by the first step, and second step will
Net ginseng/american ginseng granules are crushed to the little particle that particle diameter is 0.3mm-15 μm, and the 3rd step continues net small of ginseng/American Ginseng
Grain is crushed to nano particle, produces ginseng/American Ginseng nanometer powder.
5. the preparation method of ginseng/American Ginseng nanometer decomposing and fermenting extract according to claim any one of 1-4, it is special
Sign is, in the step (3), the inoculum density of probiotics is 1%~10%, and saccharomycete inoculum density is 1%~10%.
6. the preparation method of ginseng according to claim 5/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, institute
State in step (3), per in 1kg ginsengs/American Ginseng nanometer powder, nitrogen source addition is 1-5 parts, carbon source and water 95-99 parts.
7. the preparation method of ginseng according to claim 6/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, institute
State nitrogen source and include but is not limited to soybean protein, the carbon source includes but is not limited to granulated sugar.
8. the preparation method of ginseng according to claim 1/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, institute
State in step (4), fermentation materials fermentation time is 10-40 days.
9. the preparation method of ginseng according to claim 1/American Ginseng nanometer decomposing and fermenting extract, it is characterized in that, also
Including step (6) powder processed：Ginseng/American Ginseng enzyme liquid and excipient are well mixed, and ginseng/American Ginseng ferment is obtained after freeze-drying
Plain powder.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872647A (en) * | 2015-04-24 | 2015-09-02 | 西藏月王生物技术有限公司 | Enzyme containing medicinal and edible homologous plants and preparation method of enzyme |
| CN106498018A (en) * | 2016-11-07 | 2017-03-15 | 江南大学 | A kind of method that compound bacteria prepares the rare anticancer saponin Compound K of Radix Ginseng |
-
2017
- 2017-10-24 CN CN201711000423.3A patent/CN107737145A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872647A (en) * | 2015-04-24 | 2015-09-02 | 西藏月王生物技术有限公司 | Enzyme containing medicinal and edible homologous plants and preparation method of enzyme |
| CN106498018A (en) * | 2016-11-07 | 2017-03-15 | 江南大学 | A kind of method that compound bacteria prepares the rare anticancer saponin Compound K of Radix Ginseng |
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