CN107736534A - A kind of matrimony vine and sea-buckthorn beverage and preparation method thereof - Google Patents
A kind of matrimony vine and sea-buckthorn beverage and preparation method thereof Download PDFInfo
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- CN107736534A CN107736534A CN201711126210.5A CN201711126210A CN107736534A CN 107736534 A CN107736534 A CN 107736534A CN 201711126210 A CN201711126210 A CN 201711126210A CN 107736534 A CN107736534 A CN 107736534A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of matrimony vine and sea-buckthorn beverage, and it is prepared by the raw material of following parts by weight：10 20 parts of matrimony vine, 8 10 parts of sea-buckthorn, and corresponding preparation method is employed, the present invention has the effect of preventing and treating cardio-cerebral diseases.
Description
Technical field
The invention belongs to health product technology field, and in particular to a kind of matrimony vine and sea-buckthorn beverage and preparation method thereof.
Background technology
Ferment (ferment nutrition) refers to that plant carries out submerged fermentation, a kind of matters of containing biological activities of extraction
Less salt liquid.Above-mentioned bioactive ingredients comprise at least enzyme (enzyme), fermentation participates in bacterium.The bioactivity contained in ferment
Composition, the organized enzyme in user's body can be influenceed, from the vital movement of cell aspect regulation body.The prebiotic ferment that anaerobism makes
In, mainly probiotics and food materials, both side effects is all almost nil.Therefore, prebiotic ferment is the peace for having vitality
Full health products.But wind ferment is drunk, mycotoxin can not be avoided, can not definitely give people to eat.It is only limitted to soil improvement, eco-species
Plant, ecologic breeding.Ferment (ferment nutrition) reasonable selection food materials and zymotechnique, it can be formed respectively a kind of or more
The magical effect of kind.Including：Adjustment immune response, anticancer, hypotensive, whole intestines, constipation is eliminated, gout, diabetes B is resisted, subtracts
Fertilizer, antiallergy, as unique health products with vitality, ferment has huge health prospect.
The content of the invention
Goal of the invention：The invention provides a kind of matrimony vine and sea-buckthorn beverage and preparation method thereof.
Technical scheme：A kind of matrimony vine and sea-buckthorn beverage, it is prepared by the raw material of following parts by weight：Matrimony vine
10-20 parts, sea-buckthorn 8-10 parts.
The matrimony vine and sea-buckthorn beverage, it is prepared by the raw material of following parts by weight：15 parts of matrimony vine, sea-buckthorn 9
Part.
The matrimony vine and sea-buckthorn beverage, preparation method comprise the following steps：1. a matrimony vine and sea-buckthorn wash clean, cut
It is broken, it is put into together with sugar in bottle and adds water, stirring and deflation, ferments to obtain matrimony vine and sea-buckthorn beverage.
The matrimony vine and sea-buckthorn beverage, sugar is brown sugar in preparation method, and dosage is matrimony vine and sea-buckthorn gross weight
20%.
The matrimony vine and sea-buckthorn beverage are preparing the application in preventing and treating cardio-cerebral diseases health products.
Beneficial effect：The present invention has the function that obvious anti-cerebral ischemia and myocardial ischemia.
Embodiment
Form is described in further detail again to the above of the present invention by the following examples, but should not manage this
The scope solved as the above-mentioned theme of the present invention is only limitted to following embodiment, and all technologies for being realized based on the above of the present invention are equal
Belong to the scope of the present invention.
Embodiment 1：100 parts of matrimony vine, 100 parts of sea-buckthorn are taken, wash clean, chopping, is put into together with 40 portions of sugar in bottle and adds water, stirred
Deflation is mixed and stirred, ferment to obtain matrimony vine and sea-buckthorn beverage.
Embodiment 2：100 parts of matrimony vine, 80 parts of sea-buckthorn are taken, wash clean, chopping, is put into together with 36 portions of sugar in bottle and adds water, stirred
Deflation is mixed and stirred, ferment to obtain matrimony vine and sea-buckthorn beverage.
Embodiment 3：200 parts of matrimony vine, 100 parts of sea-buckthorn are taken, wash clean, chopping, is put into together with 60 portions of sugar in bottle and adds water, stirred
Deflation is mixed and stirred, ferment to obtain matrimony vine and sea-buckthorn beverage.
Embodiment 4：200 parts of matrimony vine, 80 parts of sea-buckthorn are taken, wash clean, chopping, is put into together with 56 portions of sugar in bottle and adds water, stirred
Deflation is mixed and stirred, ferment to obtain matrimony vine and sea-buckthorn beverage.
Embodiment 5：Influence of the present invention to Brain Tissues after Experimental Complete Cerebral Ischemia in Rats
(1) health products are tested：
The present invention, prepared by the method for above-described embodiment 2, nimodipine tablet, the limited public affairs of Shanxi Ya Bao medicine companies group share
Department's production, lot number：20160420, authentication code：The quasi- word H14022821 of medicine, 20mg*50 pieces.
(2) key instrument and reagent
Instrument：Electronic analytical balance, electronic scale, timer, electric knife, Shijiazhuang Heng Xing Medical Devices Co., Ltd.s.
Reagent：Chloraldurate, Nanjing chemical reagent factory, lot number：20100923；2,4,5- triphenyltetrazolium chlorides (TTC), the emerging reality of Shanghai section
Yan Shi equipment Co., Ltd, lot number：20101203.
(3) experimental animal
Healthy SD rat 60, all-male, the scholar 20g of body weight 180, carried by Shanghai Slac Experimental Animal Co., Ltd.
For production licence：SCXK (Shanghai) 2007-0005.
(4) animal feeding
Tested after free diets 5d, 23 DEG C of constant temperature, humidity 60%, 12 hour daytime of artificial light and 12 hours
Night replaces, and gives conventional laboratory diet, not water restriction.
(5) experimental method
1. tested material dosage is set：Basic, normal, high dosage group dosage is respectively 5ml/kg, 10ml/kg, 20ml/kg；Set up
Positive drug group, positive drug are nimodipine tablet, and adult calculates for 20mg daily, and it is 20*0.018=to be converted to rat dosage
0.36mg, i.e., 200 grams every of rat take 0.36mg Nimodipines, are converted to per kilogram dosage 1.8mg/kg.
2. animal model, packet and gavage：SD male rats are taken, artery electricity in rat brain is carried out by Tamura etc. method
Solidifying art, each mouse is with 10% chloral hydrate anesthesia (4ml/kg, intraperitoneal injection), with lateral position along right external auditory canal and right eye outer canthus line
Midpoint, cut skin about 2cm perpendicular to line, successively separating muscle, remove endocranium, artery in exposure, coagulation after confirmation
Tractus olfactorius to one section of arteria cerebri media between venae cerebri inferiores, layer-by-layer suture wound, injection 5000 units/ml Benzylpenicillin sodium salts prevents
Infection (3ml/kg, intraperitoneal injection), it is postoperative to steam again raising.Postoperative 4h, 8h, 24h rat behavior are observed, and its behavior is carried out
Scoring.
Standards of grading are:
1. carrying rat-tail about 1 chi from the ground, forelimb situation is observed.Such as two forelimbs symmetrically stretch to ground, are calculated as 0 point.If any
Wrist flexing, elbow flexing, shoulder inward turning or three, which occurs, in left fore all to be had, and is chosen as 1,2,3,4 point respectively.
2. animal horizontalization is slided on floor, left or right shoulder is pushed away respectively and is moved to offside, check the resistance that resistance promotes.Normally
Rats with bilateral resistance is symmetrical.When right shoulder moves to the left, find drop in resistance person, according to decline degree difference (it is light, in,
Weight), 1,2,3 point is chosen as respectively；
3. the forelimb of animal two is put on a wire netting, the Muscle tensility of two forelimbs is observed.The forelimb Muscle tensility pair of normal rat two
Claim.The generation left fore Muscle tensility person of being decreased obviously, according to the weight for declining degree, it is chosen as 1~3 point.
4. animal is 0 point without the person of turn-taking, the turn-take meter 1 of behavior of oriented side divides, and oriented side does not stall circle, and person counts 2 points.
According to above scale, full marks 12 divide, and fraction is higher, illustrate that the behavior disorder of animal is more serious, take 10 points with
Upper person rat is further tested.Rat is randomly divided into 6 groups, respectively sham-operation group, model group, positive drug group, this hair
Bright high, medium and low three dosage groups (medicine is prepared and dosage see on), another sham-operation group is finds arteria cerebri media without electricity
Solidifying, daily administration is secondary, continuous seven days.
3. infarct indexes determine
Fasting after administration in 7th day, secondary morning are administered latter hour, scored again by above standards of grading, it is found that each group is equal
Making moderate progress, arteria carotis is taken after blood with rat broken end is taken into brain, after removing olfactory bulb, cerebellum and low brain stem, is freezed ten minutes,
It is cut into five.1st knife is before brain among pole and optic chiasma line；2nd knife is at optic chiasma position；3rd knife is at infundibular stalk position；The
4 knives are between infundibular stalk and rear leaf tail pole.Then brain piece is placed in 5ml rapidly and contains 0.5%2,4,5- triphenyltetrazolium chlorides (TTC)
PBS (10%) solution in, 37 DEG C of lucifuge water-bath temperature incubate 10min, and after dyed, normal cerebral tissue is in rose, and is blocked
Organize white, and clear-cut.Infarct indexes determine：Taking-up is blotted with filter paper, is laid on black paper, is taken pictures in order, beats
Print, calculate infarct indexes.Infarct indexes=focus of infarct paper weight (g)/infraction side brain paper weight (g), the results are shown in Table 1；
Influence of the present invention of table 1 to ischemia apoplexy infarct indexes caused by artery in coagulation rat
Compared with model group,*P<0.05, * * P<0.01.Compared with sham-operation group,ΔP<0.05,ΔΔP<0.01。
Being shown by the result of table 1, can cause ischemia/reperfusion in rats headstroke in coagulation after artery, infarct indexes reach 5.86 ±
0.75, compared with model group, infarct indexes are decreased significantly high dose of the present invention, illustrate the present invention to ischemia/reperfusion in rats headstroke
There is certain therapeutic action.Positive drug nimodipine tablet has the effect of identical, and high dose group of the present invention and nimodipine tablet
Group compares that there was no significant difference, illustrates that the present invention has obvious therapeutic action to ischemia/reperfusion in rats headstroke, can significantly reduce
Infarct size.
Embodiment 6：The present invention is to rat experimental myocardial infarction model
(1) trial drug：Ibid.
(2) key instrument and reagent
Instrument：Electronic analytical balance, electronic scale, timer.
Reagent：Yellow Jackets, Nanjing chemical reagent factory, lot number：20100815；NBT, Shanghai is such as
Lucky biotechnology Development Co., Ltd, lot number：20110312.
(3) experimental animal
Healthy SD rat 60, all-male, the scholar 20g of body weight 180, carried by Shanghai Slac Experimental Animal Co., Ltd.
For production licence：SCXK (Shanghai) 2007-0005.
(4) animal feeding
Tested after free diets 5d, 23 DEG C of constant temperature, humidity 60%, 12 hour daytime of artificial light and 12 hours
Night replaces, and gives conventional laboratory diet, not water restriction.
(5) experimental method
1. tested material dosage is set：Ibid
2. animal model is grouped and gavage：SD rat yellow Jackets intraperitoneal injection of anesthesia (45mg/kg) is faced upward position and fixed.Gas
Cannula, make 2cm longitudinal incision on the left of breastbone, the 3rd, the 4th costal cartilage is cut in nearly breastbone side, and after opening thoracic cavity, connection is artificial
Lung ventilator (throughput 2m1/100g, 50 times/min), cut off pericardium, exposure heart, coronary artery left anterior descending branch root threading
In case ligation, record standard II lead electrocardiogram, stablizes 10 minutes, following coronary artery occlusion left anterior descending branch, thoracic cavity is closed, uses syringe
Animal throat portion secretion is suctioned out, animal is recovered autonomous respiration, after following coronary artery occlusion 15min, intravenously administrable is coronal to pricking
After artery 4 hours, heart is won, crosscutting 5 below ligature, NBT (N-BT) dyeing is carried out, calculates
Myocardial infarction area area accounts for the percentage of ventricle and heart area, and carries out statistical procedures, the results are shown in Table 2.
Influence of the present invention of table 2 to rat experimental myocardial infarction
Compared with model group,*P<0.05, * * P<0.01.Compared with sham-operation group,ΔP<0.05,ΔΔP<0.01。
Result of the test and conclusion：There were significant differences compared with sham-operation group for model group, illustrates modeling success (p<0.05) it is, high
Dosage group reduces ventricle infarction index (p<And cardiac infarction index (p 0.01)<0.01), the prompting present invention has the obvious anti-heart
Myocardial ischemia acts on, and treats rat experimental myocardial infarction.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair
The restriction of embodiments of the present invention.For those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.And these
The obvious changes or variations that the connotation for belonging to of the invention is extended out still falls within protection scope of the present invention.
Claims (5)
1. a kind of matrimony vine and sea-buckthorn beverage, it is characterised in that it is prepared by the raw material of following parts by weight：Matrimony vine
10-20 parts, sea-buckthorn 8-10 parts.
2. matrimony vine as claimed in claim 1 and sea-buckthorn beverage, it is characterised in that it is prepared by the raw material of following parts by weight
Obtain：15 parts of matrimony vine, 9 parts of sea-buckthorn.
3. matrimony vine as claimed in claim 1 and sea-buckthorn beverage, it is characterised in that preparation method comprises the following steps：1.
Matrimony vine and sea-buckthorn wash clean, chopping, are put into bottle together with sugar and add water, stirring and deflation, ferment to obtain matrimony vine and sea-buckthorn drink
Material.
4. matrimony vine as claimed in claim 1 and sea-buckthorn beverage, it is characterised in that sugar is brown sugar in preparation method, and dosage is
Matrimony vine and the 20% of sea-buckthorn gross weight.
5. matrimony vine as claimed in claim 1 and sea-buckthorn beverage are preparing the application in preventing and treating cardio-cerebral diseases health products.
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| CN201711126210.5A CN107736534A (en) | 2017-11-15 | 2017-11-15 | A kind of matrimony vine and sea-buckthorn beverage and preparation method thereof |
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| CN201711126210.5A CN107736534A (en) | 2017-11-15 | 2017-11-15 | A kind of matrimony vine and sea-buckthorn beverage and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463752A (en) * | 2019-01-03 | 2019-03-15 | 山西金科海生物制品有限公司 | A kind of sea-buckthorn composite enzyme and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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