CN107727641A - A kind of quick detection Trace Elements in Blood method and its mancarried device - Google Patents
A kind of quick detection Trace Elements in Blood method and its mancarried device Download PDFInfo
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- CN107727641A CN107727641A CN201710861816.7A CN201710861816A CN107727641A CN 107727641 A CN107727641 A CN 107727641A CN 201710861816 A CN201710861816 A CN 201710861816A CN 107727641 A CN107727641 A CN 107727641A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/71—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited
- G01N21/73—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light thermally excited using plasma burners or torches
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of quick detection Trace Elements in Blood method and its mancarried device, pass through specimen sampling, the pretreatment of sample and the step of trace element detection three, pass through the detection of portable inductive coupling plasma emission spectrograph, easily draw each micro- content in blood sample, these contents and each micronutrient levels of blood standards are contrasted, compare each micro- difference in each age group blood, difference for containing trace element in each age group blood, readjust the standard of each age group Trace Elements in Blood content, this standard removes each micro- maximum and minimum value of each age group, take standard value of the average value of remaining three values as this constituent content, it is easy to carry out the people of each age group most accurately treatment.
Description
Technical field
The present invention relates to blood testing technical field, specially a kind of quick detection Trace Elements in Blood method and its just
Portable device.
Background technology
The content height of each element and the health condition of people have direct correlation in blood, and some constituent contents can excessively cause
Poisoning, such as lead, the very few health for influenceing people of some constituent contents, such as calcium constituent, the balance of each element in blood
Most important, each element is used for quickly detecting in needing to blood in physical examination, in order to understand health situation, is entered in time
The corresponding treatment of row, but each micro- content difference in people's blood of each age group, are not easy to people's progress
Accurately judge, in order to solve the above problems, we have proposed a kind of quick detection Trace Elements in Blood method and its portable
Formula device.
The content of the invention
It is an object of the invention to provide a kind of quick detection Trace Elements in Blood method and its mancarried device, with solution
The problem of being proposed in certainly above-mentioned background technology.
To achieve the above object, the present invention provides following technical scheme：A kind of quick detection Trace Elements in Blood method,
Comprise the steps：
The first step specimen sampling, each five of the people of children, youth, centre and old four different age groups is chosen,
Their early morning fasting blood sample is gathered, blood plasma is isolated and is preserved；
The pretreatment of the second step sample, blood sample is taken out from refrigerator, is respectively implanted in beaker, in each burning
The label of corresponding packet is sticked on cup, hydrogen peroxide, nitric acid are sequentially added into each beaker, beaker is stood in refrigerator,
To take out the beaker equipped with sample again from refrigerator, beaker is grouped at random, packet five, which is placed on control-temperature electric heating plate, heats,
Solution in each beaker is transferred to be measured in the volumetric flask of identical numbering；
The 3rd step trace element detection, the solution in volumetric flask is inserted inductively coupled plasma transmitting light successively
Detected in spectrometer, difference and note between contrast all ages and classes packet Elements in Blood content and Standard blood constituent content
Record is on notebook.
Preferably, the first step specimen sampling is specially：Choose children, youth, centre and old four all ages and classes
Each five of the people of section, gathers their early morning fasting blood sample 3-5ml using blood taking needle and is respectively implanted in anticoagulant heparin pipe, utilizes
Supercentrifuge carries out centrifugal treating to these samples respectively, the serum and blood plasma of separating sample, phase is sticked to five groups of blood plasma
The label answered, plasma sample is put into 4 degrees Celsius of cryogenic box and preserved.
Preferably, the pretreatment of the second step sample is specially：Blood sample is taken out from refrigerator, is respectively implanted
In 30ml beaker, the label of corresponding packet is sticked on each beaker, 35% peroxidating is sequentially added into each beaker
Hydrogen 3ml, 60ml nitric acid 3ml, beaker internal solution is stirred evenly using glass bar, and beaker, which is stood in 4 degrees Celsius of refrigerators, fills it
The beaker equipped with sample is taken out again from refrigerator after dividing dissolving 24 hours, 24 hours, beaker is grouped at random, and packet five is put
In on control-temperature electric heating plate, making control-temperature electric heating plate temperature be maintained at 150 degrees Celsius 2 hours, the solution in each beaker is transferred to
In the 3ml volumetric flasks of identical numbering, beaker and corresponding capacity bottleneck 3 times are rinsed with a small amount of high purity water in transfer process, it
The solution in each volumetric flask is settled to 3ml with high purity water afterwards, stirred with glass bar to be measured；
Preferably, the 3rd step trace element detection is specially：Solution in volumetric flask is inserted inductive successively
Being detected in plasma emission spectrometer, the operating power of inductive coupling plasma emission spectrograph is 800-1200W,
Contrast all ages and classes are grouped the difference between Elements in Blood content and Standard blood constituent content and are recorded on notebook.
Preferably, the rotating speed of the supercentrifuge is 4000r/min, and centrifugation time is 5 minutes.
Preferably, anticoagulant heparin pipe, beaker, volumetric flask and the glass bar are rinsed before use by clear water, are used
Dried afterwards by 60% nitric acid dousing 24 hours after then being rinsed by high purity water.
Preferably, aforesaid operations are carried out in vent cabinet.
Preferably, be children less than 18 years old, 19 to 44 years old for youth, 45 to 59 years old for the middle age, more than 60 years old
For old age.
Preferably, universal wheel is installed with the lower surface corner of the inductive coupling plasma emission spectrograph,
The right flank upper end front and rear sides of the inductive coupling plasma emission spectrograph are hinged with rotation handrail, and two rotations
Laterally it is installed with cross bar between handrail, fixedly sleeved rubber sleeve at the outer side center of the cross bar.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention devises micro in a kind of quick detection blood
Element method and its mancarried device, the collection of blood sample is carried out to the crowd of different age group, these blood samples are entered
It is put into portable inductive coupling plasma emission spectrograph and is detected after row pretreatment, is easily drawn in blood sample
Each micro- content, these contents and each micronutrient levels of blood standards are contrasted, compare each age group blood
Each micro- difference in liquid, the difference for containing trace element in each age group blood, readjusts each age group blood
The standard of micronutrient levels in liquid, this standard are removed each micro- maximum and minimum value of each age group, taken surplus
Standard value of the average value of excess-three value as this constituent content, is easy to most accurately control the people of each age group
Treat.
Brief description of the drawings
Fig. 1 is inductive coupling plasma emission spectrograph structural representation in this patent.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited to
This.
A kind of quick detection Trace Elements in Blood method, comprises the steps：
The first step specimen sampling, each five of the people of children, youth, centre and old four different age groups is chosen,
Their early morning fasting blood sample is gathered, blood plasma is isolated and is preserved；
The pretreatment of the second step sample, blood sample is taken out from refrigerator, is respectively implanted in beaker, in each burning
The label of corresponding packet is sticked on cup, hydrogen peroxide, nitric acid are sequentially added into each beaker, beaker is stood in refrigerator,
To take out the beaker equipped with sample again from refrigerator, beaker is grouped at random, packet five, which is placed on control-temperature electric heating plate, heats,
Solution in each beaker is transferred to be measured in the volumetric flask of identical numbering；
The 3rd step trace element detection, the solution in volumetric flask is inserted inductively coupled plasma transmitting light successively
Detected in spectrometer, difference and note between contrast all ages and classes packet Elements in Blood content and Standard blood constituent content
Record is on notebook.
Specifically, the first step specimen sampling is specially：Choose children, youth, centre and old four not the same years
Each five of the people of age section, gather their early morning fasting blood sample 3-5ml using blood taking needle and be respectively implanted in anticoagulant heparin pipe, profit
These samples are carried out with centrifugal treating respectively with supercentrifuge, the serum and blood plasma of separating sample, five groups of blood plasma is sticked
Corresponding label, plasma sample is put into 4 degrees Celsius of cryogenic box and preserved.
Specifically, the pretreatment of the second step sample is specially：Blood sample is taken out from refrigerator, is respectively implanted
In 30ml beaker, the label of corresponding packet is sticked on each beaker, 35% peroxidating is sequentially added into each beaker
Hydrogen 3ml, 60ml nitric acid 3ml, beaker internal solution is stirred evenly using glass bar, and beaker, which is stood in 4 degrees Celsius of refrigerators, fills it
The beaker equipped with sample is taken out again from refrigerator after dividing dissolving 24 hours, 24 hours, beaker is grouped at random, and packet five is put
In on control-temperature electric heating plate, making control-temperature electric heating plate temperature be maintained at 150 degrees Celsius 2 hours, the solution in each beaker is transferred to
In the 3ml volumetric flasks of identical numbering, beaker and corresponding capacity bottleneck 3 times are rinsed with a small amount of high purity water in transfer process, it
The solution in each volumetric flask is settled to 3ml with high purity water afterwards, stirred with glass bar to be measured；
Specifically, the 3rd step trace element detection is specially：Solution in volumetric flask is inserted inductance coupling successively
Close and detected in plasma emission spectrometer, the operating power of inductive coupling plasma emission spectrograph is 800-
1200W, contrast all ages and classes are grouped the difference between Elements in Blood content and Standard blood constituent content and are recorded in notes
In sheet.
Specifically, the rotating speed of the supercentrifuge is 4000r/min, and centrifugation time is 5 minutes.
Specifically, anticoagulant heparin pipe, beaker, volumetric flask and the glass bar are rinsed before use by clear water, are made
With rear by 60% nitric acid dousing 24 hours, dried after then being rinsed by high purity water.
Specifically, aforesaid operations are carried out in vent cabinet.
Specifically, be children less than 18 years old, 19 to 44 years old for youth, 45 to 59 years old for the middle age, more than 60 years old
For old age.
Specifically, it is installed with the lower surface corner of the inductive coupling plasma emission spectrograph universal
Wheel, the right flank upper end front and rear sides of the inductive coupling plasma emission spectrograph are hinged with rotation handrail, and two
Rotate and be laterally installed with cross bar between handrail, fixedly sleeved rubber sleeve at the outer side center of the cross bar.
Operation principle：Quick detection Trace Elements in Blood portable equipment herein is launched for inductively coupled plasma
Spectrometer, everything wheel is installed by self-inductance coupled plasma optical emission spectrometer lower surface, makes to move inductively coupled plasma
Body emission spectrometer is more convenient, while rotates handrail and can rotate upwardly and downwardly, and realizes the expansion and folding for rotating handrail, expansion turns
During dynamic handrail, whole inductively coupled plasma is promoted to launch by grasping the rubber sleeve on two Inter cross bars for rotating handrail
Spectrometer, the portable of inductive coupling plasma emission spectrograph is realized, carry out specimen sampling, the pretreatment of sample and micro- successively
Secondary element detects three steps, by the detection of portable inductive coupling plasma emission spectrograph, easily draws blood sample
In each micro- content, these contents and each micronutrient levels of blood standards are contrasted, compare each age group
Each micro- difference in blood, the difference for containing trace element in each age group blood, readjusts each age group
The standard of Trace Elements in Blood content, this standard are removed each micro- maximum and minimum value of each age group, taken
Standard value of the average value of remaining three values as this constituent content, is easy to most accurately control the people of each age group
Treat.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (9)
1. a kind of quick detection Trace Elements in Blood method, comprises the steps, it is characterised in that：
The first step specimen sampling, choose each five of the people of children, youth, centre and old four different age groups, collection
Their early morning fasting blood sample, isolates blood plasma and is preserved；
The pretreatment of the second step sample, blood sample is taken out from refrigerator, is respectively implanted in beaker, on each beaker
The label of corresponding packet is sticked, hydrogen peroxide, nitric acid are sequentially added into each beaker, beaker is stood in refrigerator, from ice
The beaker equipped with sample is taken out in case again, beaker is grouped at random, packet five, which is placed on control-temperature electric heating plate, heats, each
Solution in beaker is transferred to be measured in the volumetric flask of identical numbering；
The 3rd step trace element detection, the solution in volumetric flask is inserted inductive coupling plasma emission spectrograph successively
In detected, in contrast all ages and classes packet blood between each each micronutrient levels of micronutrient levels and Standard blood
Difference is simultaneously recorded on notebook.
A kind of 2. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that the first step
Specimen sampling is specially：Each five of the people of children, youth, centre and old four different age groups is chosen, is adopted using blood taking needle
Collect their early morning fasting blood sample 3-5ml to be respectively implanted in anticoagulant heparin pipe, these samples are distinguished using supercentrifuge
Centrifugal treating is carried out, the serum and blood plasma of separating sample, corresponding label is sticked to five groups of blood plasma, plasma sample is put into 4
Degree Celsius cryogenic box in preserve.
A kind of 3. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that the second step
The pretreatment of sample is specially：Blood sample is taken out from refrigerator, is respectively implanted in 30ml beaker, is pasted on each beaker
The label of upper corresponding packet, 35% hydrogen peroxide 3ml, 60ml nitric acid 3ml is sequentially added into each beaker, utilizes glass bar
Beaker internal solution is stirred evenly, beaker is stood in 4 degrees Celsius of refrigerators, is taken out again equipped with sample from refrigerator after 24 hours
Beaker, beaker is grouped at random, packet five is placed on control-temperature electric heating plate, control-temperature electric heating plate temperature is maintained at 150 degree Celsius 2
Hour, the solution in each beaker is transferred in the 3ml volumetric flasks of identical numbering, rushed in transfer process with a small amount of high purity water
Beaker and corresponding capacity bottleneck 3 times are washed, the solution in each volumetric flask is settled to 3ml with high purity water afterwards, uses glass bar
Stir to be measured.
A kind of 4. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that the 3rd step
Trace element detection is specially：Solution in volumetric flask is inserted in inductive coupling plasma emission spectrograph successively and examined
Survey, the operating power of inductive coupling plasma emission spectrograph is 800-1200W, and member is each in contrast all ages and classes packet blood
Difference between micro each micronutrient levels of cellulose content and Standard blood is simultaneously recorded on notebook.
A kind of 5. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that：The high speed from
The rotating speed of scheming is 4000r/min, and centrifugation time is 5 minutes.
A kind of 6. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that：The heparin resists
Solidifying pipe, beaker, volumetric flask and glass bar are rinsed before use by clear water, and the nitric acid dousing 24 after use by 60% is small
When, dried after then being rinsed by high purity water.
A kind of 7. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that：Aforesaid operations are equal
Carried out in vent cabinet.
A kind of 8. quick detection Trace Elements in Blood method according to claim 1, it is characterised in that：Less than 18 years old
For children, 19 to 44 years old for youth, 45 to 59 years old for the middle age, be old age more than 60 years old.
A kind of 9. mancarried device of quick detection Trace Elements in Blood, it is characterised in that：The inductively coupled plasma
Universal wheel, the right side of the inductive coupling plasma emission spectrograph are installed with the lower surface corner of emission spectrometer
Face upper end front and rear sides are hinged with rotation handrail, and are laterally installed with cross bar, the cross bar between two rotation handrails
Outer side center at fixedly sleeved rubber sleeve.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710861816.7A CN107727641A (en) | 2017-09-21 | 2017-09-21 | A kind of quick detection Trace Elements in Blood method and its mancarried device |
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| CN201710861816.7A CN107727641A (en) | 2017-09-21 | 2017-09-21 | A kind of quick detection Trace Elements in Blood method and its mancarried device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112114023A (en) * | 2019-06-19 | 2020-12-22 | 湖北中烟工业有限责任公司 | Method for detecting aluminum release amount in smoke of cigarette heated without burning |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105784831A (en) * | 2016-03-31 | 2016-07-20 | 广州市圣鑫生物科技有限公司 | Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method |
-
2017
- 2017-09-21 CN CN201710861816.7A patent/CN107727641A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784831A (en) * | 2016-03-31 | 2016-07-20 | 广州市圣鑫生物科技有限公司 | Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method |
Non-Patent Citations (4)
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| 严海英等: "微波消解ICP-AES测定疾病人群血清中的微量元素", 《光谱实验室》 * |
| 候勇: "CYP1A1/GSTM1多态性和血浆锌/铜与非小细胞肺癌的关系研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112114023A (en) * | 2019-06-19 | 2020-12-22 | 湖北中烟工业有限责任公司 | Method for detecting aluminum release amount in smoke of cigarette heated without burning |
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