CN107714638A - A kind of lightening compositions containing fleece-flower root stem cell extract and its application - Google Patents
A kind of lightening compositions containing fleece-flower root stem cell extract and its application Download PDFInfo
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- CN107714638A CN107714638A CN201711061067.6A CN201711061067A CN107714638A CN 107714638 A CN107714638 A CN 107714638A CN 201711061067 A CN201711061067 A CN 201711061067A CN 107714638 A CN107714638 A CN 107714638A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The invention discloses a kind of lightening compositions, the composition contains fleece-flower root stem cell extract and plant extracts.Fleece-flower root stem cell extract in the present composition can quickly activate dormancy fibroblast, promote cell division, propagation, lift DNA Damage repair ability, promote cell self-regeneration renewal, constantly displacement senile cell, excludes intracellular toxin, removes free radical, it is calm to alleviate pigment color spot, so as to play preferable whitening function；Meanwhile fleece-flower root stem cell extract and plant extracts collective effect can further be played synergistic function by the present invention, promote that functional component in plant extracts acts on it is further it is more preferable play, reach more preferable whitening effect.In addition, invention additionally discloses a kind of skin care item for including the composition, the skin care item have preferable whitening effect.
Description
Technical field
The present invention relates to a kind of composition and its application, especially a kind of whitening group containing fleece-flower root stem cell extract
Compound and its application.
Background technology
Recently as there is pigment on the aging of population, environmental pollution, the increasing people's skin of global warning
Imbalance is formed, the foxiness of skin, freckle have turned into serious skin and perplexed.Particularly after foxiness, freckle and Exposure to Sunlight on skin
Black pigment calmness can increase with the age and produce, increase, while be increasingly difficult to eliminate, thus can not avoid as people
Worry.Calm black pigment is almost melanin entirely on skin, black in its melanocyte between epidermis and corium
Produced in ferritic, the melanocyte of generation is spread by osmosis to flanking cell.
In normal state, the color of human skin depends on content and the distribution of melanin, so the core of lightening mechanism
Intracardiac appearance is exactly the formation for suppressing melanin, the decomposition for accelerating melanin.Melanin is formed and the physiology course of discharge substantially may be used
It is summarised as：Aliphatic acid tyrosine generates DOPA quinone in the presence of tyrosinase, and it is under the oxidation of enzyme or non-enzymatic, warp
Haematochrome and colourless pigment are changed into melanin, and this process is the main reason of melanin.Therefore, hinder or suppress the reaction
The effect of tyrosinase in first stage, you can suppress melanogenesis, so, tyrosinase effect can be hindered or suppress by seeking
Medicine or skin care item, to prevent or suppress because tyrosinase acts on and for the melanocyte that generates it is particularly important.
As the saying goes one it is white hide it is three ugly, whitening into many people seeking beauties hope, therefore, it is necessary to research and develop a kind of U.S.
White skin care item, tyrosinase activity can be hindered, or act on each stage of melanin metabolic pathway, controlled, suppress black
The generation of pigment, inherently reach preferable whitening effect.
The content of the invention
Based on this, one kind is provided and contains the fleece-flower root it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
The lightening compositions of stem cell extract.
To achieve the above object, the technical solution used in the present invention is：A kind of lightening compositions, the composition contain
Fleece-flower root stem cell extract and plant extracts.
Fleece-flower root stem cell extract of the present invention can quickly activate dormancy fibroblast, promote cell division,
Propagation, DNA Damage repair ability is lifted, promotes cell self-regeneration renewal, secrete Collagen material, adjust collagenous fibres,
Strengthen skin elasticity, there is strong anti-oxidation, constantly replace senile cell, exclude intracellular toxin, remove free radical, alleviate color
Plain color spot is calm, so as to play preferable whitening function.
In addition, most of all, present inventor passes through a large amount of exploration discoveries, in the present composition, the fleece-flower root is done
Cell extract can be effectively promoted plant extracts and play nutritive effect effect, play surprising white-skinned face function.
Fleece-flower root stem cell extract of the present invention is prepared by the following method gained：
1) separation and culture of fleece-flower root forming layer stem cell：By the root tuber of the fleece-flower root after disinfecting, bag is cut
Include the semicircle section including forming layer；Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；Will after two weeks
The outer paraphyte of forming layer had significant proliferation takes out, and separates forming layer stem cell and is transferred in subculture medium and cultivate, every two weeks
Subculture once, obtains fleece-flower root forming layer stem cell；
2) culture medium after culture fleece-flower root forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of fleece-flower root forming layer stem cell is dry
It is dry, obtain fleece-flower root stem cell extract.
Preferably, the weight ratio of the fleece-flower root stem cell extract and plant extracts is：Fleece-flower root stem cell is extracted
Thing：Plant extracts=(3~15)：40.Above-mentioned weight than fleece-flower root stem cell and plant extracts, can preferably play
Synergistic function, play preferable white-skinned face function.
It is highly preferred that the weight ratio of the fleece-flower root stem cell extract and plant extracts is：Fleece-flower root stem cell carries
Take thing：Plant extracts=5：40.Above-mentioned weight than fleece-flower root stem cell and plant extracts, can be best performance collaboration
Synergistic effect, play more preferable white-skinned face function.
Preferably, the plant extracts is Semen Coicis extract, roselle extract, Rosa centifolia flower extract, mulberries
At least one of extract.
The selection of the above-mentioned plant extracts of the present invention, with fleece-flower root stem cell collective effect, can play more preferable whitening work(
Effect.
It is highly preferred that the plant extracts is Semen Coicis extract, roselle extract, Rosa centifolia flower extract, mulberry
The compounding of Shen extracts.
Present inventor is had found, Semen Coicis extract, roselle extract, Rosa centifolia flower extract, mulberries are extracted
Thing is used cooperatively, and is mutually promoted, and can play synergistic function, reaches preferable whitening effect, wherein,
Semen Coicis extract：All on the books in China's medical book ancient books, coix seed is splendid beauty food materials, is put down with wart is controlled
The maintaining beauty and keeping youngs such as acne, light speckles, beautification and white, moisturizing smoothing wrinkle, especially contained proteolytic enzyme can make skin keratin soft
Change, vitamin E Wheat Protein.《Compendium of Materia Medica》Call adlay " invigorating spleen and reinforcing stomach, tonifying lung heat-clearing, expelling wind and eliminating dampness, beauty treatment stay hold,
Make light of one's life by commiting suicide macrobiosis ".
Roselle extract：For the flower of malvaceae plant roselle, there is soothing the liver fall fire, heat clearing and inflammation relieving, refreshment and tranquilization, clear
Except free radical etc. acts on, there is preferable inoxidizability.
Rosa centifolia flower extract：Rosaceous plant has indomitable vitality always, the resistance for improving skin itself
Power plays immeasurable effect, and Rosa centifolia has preferable whitening spot-removing effect.
Mulberries extract：Mulberries have improvement skin blood supply, nutrition skin, make skin delicate, and energy anti-aging, are
The good medicine of U.S. face, anti-aging, and contain abundant activated protein, vitamin, amino acid, malic acid, carrotene, mineral matter,
Nutrition is 5-6 times of apple, is 4 times of grape, has multiple efficacies.
It is highly preferred that the Semen Coicis extract, roselle extract, Rosa centifolia flower extract, the weight of mulberries extract
Measuring ratio is：Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：Mulberries extract=(3~8):5:5:9.It is above-mentioned
Weight than four kinds of compositions, Synergistic, there is more preferable whitening effect.
It is highly preferred that the Semen Coicis extract, roselle extract, Rosa centifolia flower extract, the weight of mulberries extract
Measuring ratio is：Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：Mulberries extract=4:5:5:9.Above-mentioned weight
Four kinds of compositions of ratio, Synergistic, there is best whitening effect.
Meanwhile invention additionally discloses a kind of skin care item for including the lightening compositions.The skin care item can be from cell generation
The level of thanking is set out, and is promoted cell metabolism, purifying skin, is played preferable whitening effect.
A kind of in addition, application invention additionally discloses composition in terms of skin care.The composition is applied to skin care
Aspect, preferable whitening effect can be played.
Preferably, weight/mass percentage composition of the fleece-flower root stem cell extract in the skin care item is 0.01~3%.
Present inventor has found, the fleece-flower root stem cell of above-mentioned content, preferably can promote other in skin care item
Composition plays a role, and plays more preferable skin effect.
In addition, it is noted that skin care item of the present invention can also include auxiliary material, the selection of auxiliary material has no tool herein
Body limits, and those skilled in the art can carry out conventional selection such as water, alcohols, finish, surfactant, pH according to being actually needed
The auxiliary materials such as conditioning agent, antioxidant or preservative.In addition, skin care item of the present invention can be made creme, emulsion, essence lotion,
The diversified forms such as gel or facial mask, the present invention have no specific restriction to this, and those skilled in the art can also be according to actual need
Carry out conventional selection.
Relative to prior art, beneficial effects of the present invention are：
Fleece-flower root stem cell extract in the present composition can quickly activate dormancy fibroblast, promote cell
Division, propagation, lift DNA Damage repair ability, promote cell self-regeneration renewal, secrete Collagen material, adjust collagen
Fiber, strengthen skin elasticity, there is strong anti-oxidation, constantly replace senile cell, exclude intracellular toxin, remove free radical,
It is calm to alleviate pigment color spot, so as to play preferable whitening function.
Semen Coicis extract of the present invention, roselle extract, Rosa centifolia flower extract, four kinds of mulberries extract into
Point, synergistic function can be preferably played, plays preferable white-skinned face function.
Meanwhile by fleece-flower root stem cell extract and plant extracts collective effect, it can further play Synergistic
Effect, promote the further more preferable performance that functional component acts in plant extracts, so as to reach more preferable whitening effect.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：5 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=4:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, the essence
Magnificent liquid also includes the composition of following weight/mass percentage composition：Glycerine 5%, glycine betaine 2.5%, carbomer 0.35%, hydrolysis Jojoba
Esters 0.35%, Sodium Hyaluronate 0.04%, essence and Preservative qs, surplus are water, the fleece-flower root stem cell extract
Weight/mass percentage composition in the skin care item is 2%.
Each composition is prepared into Essence by Essence described in the present embodiment according to this area conventional method.
Embodiment 2
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：3 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=4:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 2%, the other compositions of the Essence and preparation
Method is and embodiment 1 is identical.
Embodiment 3
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：15 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia is extracted
Thing：Mulberries extract=4:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 2%, the other compositions of the Essence and preparation
Method is and embodiment 1 is identical.
Embodiment 4
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：2 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=4:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 0.01%, the composition of the Essence and preparation side
Method is and embodiment 1 is identical.
Embodiment 5
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：16 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia is extracted
Thing：Mulberries extract=4:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 3%, the composition and preparation method of the Essence
And embodiment 1 is identical.
Embodiment 6
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：5 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=3:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 2%, the other compositions of the Essence and preparation
Method is and embodiment 1 is identical.
Embodiment 7
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：5 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=8:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 2%, the other compositions of the Essence and preparation
Method is and embodiment 1 is identical.
Embodiment 8
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：5 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=2:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 2%, the other compositions of the Essence and preparation
Method is and embodiment 1 is identical.
Embodiment 9
A kind of embodiment of lightening compositions of the present invention, composition described in the present embodiment include following parts by weight into
Point：5 parts of fleece-flower root stem cell extract, plant extracts (Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：
Mulberries extract=9:5:5:9) 40 parts.
A kind of skin care item for including the lightening compositions of the present embodiment, the skin care item are a Essence, it is described what
Weight/mass percentage composition of the tuber of multiflower knotweed stem cell extract in the skin care item is 2%, the other compositions of the Essence and preparation
Method is and embodiment 1 is identical.
Embodiment 10
Whitening effect is evaluated
Experimental selection female volunteers, melanin content in human body skin is measured, the white-skinned face function of skin-protection product is carried out
Evaluation., with after continuous use skin care item 21 days, determined using 1 day before skin care item using MEXAMETER MX18 skin pigments instrument
The melanin content of its hand skin.Determination of the environment：Test environment temperature (22 ± 1) DEG C, humidity (50 ± 5) %, averages.
Study subject uses a skin care item product daily, and application method is：After cleaning hand skin, dry, applied cosmetics with hand
It is put on hand skin.Study subject is during test without using other whitening products.Measured value is higher, illustrates black in skin
Pigment content is higher.Wherein, melanin reduced rate=(use the melanin content-continuous use cosmetics 21 of 1 day before cosmetics
Melanin content after it) ÷ uses melanin content × 100% of 1 day before cosmetics.Melanin reduced rate is higher, illustrates whitening
Effect is more obvious.The whitening effect of cleawhite over-night essences in the embodiment of the present invention 1~9 is measured respectively using the above method,
And control group is set, for control group in addition to fleece-flower root stem cell is not contained, remaining content is same as Example 1, as a result such as the institute of table 1
Show：
The melanin reduced rate situation of table 1
| Group | Melanin reduced rate, % |
| Embodiment 1 | 45.8 |
| Embodiment 2 | 31.6 |
| Embodiment 3 | 32.7 |
| Embodiment 4 | 25.8 |
| Embodiment 5 | 26.1 |
| Embodiment 6 | 36.7 |
| Embodiment 7 | 35.9 |
| Embodiment 8 | 30.2 |
| Embodiment 9 | 29.9 |
| Control group | 16.8 |
As it can be seen from table 1 compared with control group, the embodiment of the present invention 1~9 is respectively provided with the effect for preferably reducing melanin
Fruit, this illustrates that fleece-flower root stem cell of the present invention can further promote functional component in plant extracts to play a role, wherein, it is real
The melanin reducing effect for applying example 1 is most obvious；Compared with embodiment 4 and embodiment 5, embodiment 2 and embodiment 3 have preferably
Melanin effect is reduced, embodiment 1 has preferably reduction melanin effect, and this is also the preferred fleece-flower root in the present composition
Stem cell extract：Plant extracts=(3~15)：40, and further preferably fleece-flower root stem cell extract：Plant extracts
=5：40 the reason for；Compared with embodiment 8 and embodiment 9, embodiment 6 and embodiment 7 have preferably reduction melanin effect,
Embodiment 1 has preferably reduction melanin effect, and this is also preferred Semen Coicis extract in the present composition：Roselle carries
Take thing：Rosa centifolia flower extract：Mulberries extract=(3~8):5:5:9, and further preferred Semen Coicis extract：Roselle
Extract：Rosa centifolia flower extract：Mulberries extract=4:5:5:9 the reason for.
Embodiment 11
Whitening effect is verified
The white-skinned face function of composition is verified by external Tyrosinase inhibition assays.In the forming process of melanin, junket
Propylhomoserin enzyme plays a crucial role, and how much with the activity of tyrosinase has a direct relation caused by melanin, skin-whitening agents are typically logical
Cross and act in dermal melanin generation, metabolic process, suppress the generation of melanin, accelerate the metabolism of melanin and play whitening
The effect of skin.
The present embodiment sets experimental group 1~9 and control group 1~14, and experimental group 1~9 is in the embodiment of the present invention 1~9
Cleawhite over-night essences, except Semen Coicis extract, roselle extract, Rosa centifolia flower extract, mulberries extract in control group 1~14
The content ratio of four kinds of compositions it is different it is outer (weight/mass percentage composition total amount of four kinds of compositions in cleawhite over-night essences is identical, simply four
The ratio of kind of composition is different), other compositions are same as Example 1, Semen Coicis extract in specific control group 1~14,
Roselle extract, Rosa centifolia flower extract, the content ratio of four kinds of compositions of mulberries extract are as shown in table 2：
Semen Coicis extract, roselle extract, Rosa centifolia flower extract, mulberry in the control group Essence of table 2
The content ratio of four kinds of compositions of Shen extracts
| Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| Semen Coicis extract | 4 | 0 | 0 | 0 | 4 | 4 | 4 | 0 | 0 | 0 | 4 | 4 | 0 | 4 |
| Roselle extract | 0 | 5 | 0 | 0 | 5 | 0 | 0 | 5 | 5 | 0 | 5 | 5 | 5 | 0 |
| Rosa centifolia flower extract | 0 | 0 | 5 | 0 | 0 | 5 | 0 | 5 | 0 | 5 | 5 | 0 | 5 | 5 |
| Mulberries extract | 0 | 0 | 0 | 9 | 0 | 0 | 9 | 0 | 9 | 9 | 0 | 9 | 9 | 9 |
The Essence of experimental group 1~9, control group 1~14 is made into the solution that quality volume fraction is 1%, ultrasound respectively
Ripple auxiliary dissolving 1h, takes supernatant standby as sample.
In 0.9ml sample solution, 1mlL- tyrosine (0.3mol) and 1ml pH=6.8 phosphate buffer are added
(PBS), preheated 10 minutes in 37 DEG C of Water Tanks with Temp.-controlled, add the 0.1ml tyrosinase aqueous solution (1.0mg/ml, mcllvains
Buffer solution), it is sufficiently stirred, after 37 DEG C are reacted 15 minutes, determines 475nm absorbances (D1).In addition, with above-mentioned phosphate buffer
Same reaction is carried out instead of style solution, measures absorbance (D2).In order to compare, sample solution and tyrosinase are being not added with
Under the conditions of, measure absorbance (D3).Inhibitory activity against tyrosinase is calculated with following formula, wherein, inhibitory activity against tyrosinase
(%)=(D2-D1)/(D2-D3) × 100, the inhibitory activity against tyrosinase of Essence in experimental group 1~9, control group 1~14
As shown in table 3：
The inhibitory activity against tyrosinase of Essence in the experimental group 1~9 of table 3, control group 1~14
As seen from Table 3, in the case that total amount in four kinds of compositions in Essence is certain, the elite of control group 1~14
One in four kinds of Semen Coicis extract, roselle extract, Rosa centifolia flower extract, mulberries extract compositions is comprised only in liquid
Plant, two or three, suppress tyrosinase activity effect without good.And the Essence of experimental group 1~9 contain four kinds into
Point, suppress tyrosinase activity positive effect and be significantly better than control group 1~14.Thus it is provable, in Essence of the invention, the heart of a lotus seed
Four kinds of seed of jog's tears extract, roselle extract, Rosa centifolia flower extract, mulberries extract compositions, which have, mutually promotes, mutually assists
Same effect, significant suppression tyrosinase activity effect can be played.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of lightening compositions, it is characterised in that the composition contains fleece-flower root stem cell extract and plant extracts.
2. lightening compositions as claimed in claim 1, it is characterised in that the fleece-flower root stem cell extract and plant extract
The weight ratio of thing is：Fleece-flower root stem cell extract：Plant extracts=(3~15)：40.
3. lightening compositions as claimed in claim 2, it is characterised in that the fleece-flower root stem cell extract and plant extract
The weight ratio of thing is：Fleece-flower root stem cell extract：Plant extracts=5：40.
4. the lightening compositions as described in any one of claims 1 to 3, it is characterised in that the plant extracts is coix seed
At least one of extract, roselle extract, Rosa centifolia flower extract, mulberries extract.
5. lightening compositions as claimed in claim 4, it is characterised in that the plant extracts is Semen Coicis extract, rose
Rare eggplant extract, Rosa centifolia flower extract, the compounding of mulberries extract.
6. lightening compositions as claimed in claim 5, it is characterised in that the Semen Coicis extract, roselle extract, ocean
Rose flower extract, the weight ratio of mulberries extract are：Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：Mulberry
Shen extracts=(3~8):5:5:9.
7. lightening compositions as claimed in claim 6, it is characterised in that the Semen Coicis extract, roselle extract, ocean
Rose flower extract, the weight ratio of mulberries extract are：Semen Coicis extract：Roselle extract：Rosa centifolia flower extract：Mulberry
Shen extract=4:5:5:9.
A kind of 8. skin care item for including the lightening compositions as described in any one of claim 1~7.
9. application of the lightening compositions in terms of skin care as described in any one of claim 1~7.
10. skin care item as claimed in claim 8, it is characterised in that the fleece-flower root stem cell extract is in the skin care item
In weight/mass percentage composition be 0.01~3%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711061067.6A CN107714638A (en) | 2017-10-31 | 2017-10-31 | A kind of lightening compositions containing fleece-flower root stem cell extract and its application |
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| CN201711061067.6A CN107714638A (en) | 2017-10-31 | 2017-10-31 | A kind of lightening compositions containing fleece-flower root stem cell extract and its application |
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| CN107714638A true CN107714638A (en) | 2018-02-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711061067.6A Withdrawn CN107714638A (en) | 2017-10-31 | 2017-10-31 | A kind of lightening compositions containing fleece-flower root stem cell extract and its application |
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| CN (1) | CN107714638A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112716855A (en) * | 2021-01-27 | 2021-04-30 | 科丝美诗(中国)化妆品有限公司 | Composition with barrier repairing and anti-aging effects and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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2017
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (2)
| Title |
|---|
| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
| 谭凯丽 等: "何首乌的药理作用研究进展", 《山地农业生物学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112716855A (en) * | 2021-01-27 | 2021-04-30 | 科丝美诗(中国)化妆品有限公司 | Composition with barrier repairing and anti-aging effects and preparation method and application thereof |
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Application publication date: 20180223 |