CN107703201A - A kind of aquaculture persticide residue early warning system - Google Patents
A kind of aquaculture persticide residue early warning system Download PDFInfo
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- CN107703201A CN107703201A CN201710901973.6A CN201710901973A CN107703201A CN 107703201 A CN107703201 A CN 107703201A CN 201710901973 A CN201710901973 A CN 201710901973A CN 107703201 A CN107703201 A CN 107703201A
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- G—PHYSICS
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Abstract
The invention particularly discloses a kind of aquaculture persticide residue early warning system, including sample introduction module, sample message Registration Module, sample pretreatment module, detection module and summary report generation module；The sample introduction module, sample message Registration Module, sample pretreatment module, detection module and summary report generation module electrically connect with central processing unit；The sample introduction module can receive Feed Sample and animal wastes sample.The aquaculture persticide residue early warning system of the present invention, a variety of detection parameters are creatively combined, include the detection of heavy metal, the detection of residues of pesticides and the detection of residue of veterinary drug；The security of the products such as the meat, egg, milk that plant produces can at utmost be ensured.
Description
Technical field
The present invention relates to residues of pesticides early warning technology field, more particularly to a kind of aquaculture persticide residue early warning system
System.
Background technology
With the rapid development of economy, aquaculture industry of China also reaches significant progress, progressively from small-sized family-raise field
Or pasture is small-scale, change to the large-scale farming field of standardization batch production.Feed is the basis of aquaculture, and aquaculture is
It is no to develop in a healthy way, it is closely bound up with quality of the fodder.The raw material of feed is usually a variety of agricultural product, if the product of agricultural product
Matter is bad, will directly influence the quantum of output and quality of aquaculture.If the feed for having used residues of pesticides exceeded is raised,
Agricultural chemicals will be feed by animal, be directly entered the enteron aisle of animal, by being absorbed into vivo, and then pollute the meat of animal, egg,
The products such as milk.Being excessively used for veterinary drug, will also result in food pollution, endanger the health of people.
Therefore, there is an urgent need to establish the residues of pesticides early warning system of a set of aquaculture, the feed of detection aquaculture is
It is no qualified, detect, the product such as meat, egg, milk before listing is exceeded with the presence or absence of agricultural chemicals, residue of veterinary drug.
The content of the invention
For the above-mentioned deficiency of prior art, the present invention provides a kind of aquaculture persticide residue early warning system, with solution
Certainly above-mentioned technical problem.
The present invention is achieved by the following technical solutions：
A kind of aquaculture persticide residue early warning system, including sample introduction module, sample message Registration Module, specimen preprocessing
Manage module, detection module and summary report generation module；The sample introduction module, sample message Registration Module, sample pretreatment mould
Block, detection module and summary report generation module electrically connect with central processing unit；The sample introduction module can receive feed sample
Product and animal wastes sample.
Relative to prior art, beneficial effects of the present invention：
The aquaculture persticide residue early warning system of the present invention, creatively combines a variety of detection parameters,
Detection including heavy metal, the detection of residues of pesticides and the detection of residue of veterinary drug；It can at utmost ensure what plant produced
The security of the products such as meat, egg, milk.This early warning system also includes the warning system electrically connected with central processing unit, early warning system
In parameter be detected to each be both provided with corresponding safe range, generated if central processing unit receives from summary report
Testing result and exceeded situation, central processing unit in module will start modes of warning, and warning system will start working
Send corresponding alarm；Including sending information to corresponding client by network, in addition to start flicker alarm lamp and voice
Alarm unit.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Fig. 2 is full cell microorganism sensor construction schematic diagram in Detecting Pesticide module of the present invention.
Wherein：Reaction tank -1, three-electrode system -2, shower nozzle -3, exhaust system -4, cleaning fluid storage room -5, sampling system -
6, filter layer -7.
Embodiment
The invention will be further described with the following Examples.
A kind of aquaculture persticide residue early warning system, as shown in figure 1, including sample introduction module, sample message registration mould
Block, sample pretreatment module, detection module and summary report generation module；The sample introduction module, sample message Registration Module, sample
Product pretreatment module, detection module and summary report generation module electrically connect with central processing unit；The sample introduction module can be with
Receive Feed Sample and animal wastes sample.
Above-described aquaculture persticide residue early warning system, in addition to the alarm system electrically connected with central processing unit
System.Parameter is detected to each in early warning system and is both provided with corresponding safe range, is come from if central processing unit receives
Testing result and exceeded situation, central processing unit in summary report generation module will start modes of warning, warning system
It will start working and send corresponding alarm；Including sending information to corresponding client by network, in addition to start flicker
Alarm lamp and audio alert unit.
Preferably, the censorship unit of the sample message Registration Module registration sample, submission date, the form of sample, sample
The information such as product weight.
Preferably, the sample pretreatment module includes disintegrating mechanism, sieving mechanism and dilution module.
Preferably, the detection module includes heavy metal analysis module, Detecting Pesticide module and detection of veterinary drugs in food
Module；The heavy metal analysis module, Detecting Pesticide module and detection of veterinary drugs in food module are also adopted including corresponding information
Collect module, the information transfer collected is into summary report generation module.
The Detecting Pesticide module is a kind of full cell microorganism sensor, as shown in Fig. 2 including reaction tank and entering
Sample system, the reaction tank include three-electrode system, cleaning systems and exhaust system；In the three-electrode system to electrode and
Reference electrode is incorporated into the both sides of reaction tank, and is bonded with pool wall；The working electrode of three electrode is placed in the centre of reaction tank；
The cleaning systems include being arranged on the shower nozzle at four angles and coupled logical cleaning fluid storage room；The exhaust system is set
Put and reaction tank lower section, including two outlets of work waste liquid and cleaning waste liquid；The sensor electrically connects with electrochemical workstation.
Preferably, the shower nozzle includes hang down type sprinkler tip, upright sprinkler, conventional sprinkler and abutment wall type
One or more in sprinkler tip.
Preferably, the sampling system includes crude samples pond, pre-processes mechanism and detected sample pond；The sampling system
It is connected with reaction tank by conduit, flowmeter and electromagnetic valve switch is additionally provided with conduit.
Preferably, the pretreatment mechanism includes 3~5 layers of filter layer, and the diameter of the filter layer reduces successively, minimum one
The aperture of individual filter layer is less than 100um.
Preferably, described is platinum electrode to electrode；The reference electrode is solid state reference electrode.
Preferably, the working electrode is the glass-carbon electrode or platinum electrode of microorganism modified.
The operation principle of sensor of the invention：After adding all working solutions, unloaded energization 3-5min, current signal is treated
After stable, the steady-state current value Iq before measure sample-adding；The testing sample of separate sources is added, determines the electric current Ih after sample-adding；Electricity
Chem workstation record current change curve, according to the suppression curve of electric current, it can both calculate and suppress corresponding to various concentrations
Rate R (100%), inhibiting rate calculation formula are as follows：
R=(1-Ih/Iq) * 100%
Sensor of the invention includes sampling system, and sampling system can carry out multiple pretreatment to testing sample, including
The solid impurity and macromolecular substances of some bulky grains are removed, testing sample impurity after treatment is less, can be with maximum journey
Degree reduces damage of the testing sample in detection process to three-electrode system, and then provides the service life of sensor.
As preferred embodiment, the preparation process of the working electrode of the present invention, including following step are will be described in detail below
Suddenly：
S1. take glass-carbon electrode or platinum electrode to be pre-processed, carry out acid cleaning and alkali cleaning first, finally carry out clear water
Rinse；
S2. training objective microorganism, objective microbe are Escherichia coli, collect the wet thallus of objective microbe, 4 degree of preservations
It is standby；
S3. graft polymers PVA-g-P is prepared；
S4. will be sufficiently mixed in the wet thallus of above-mentioned steps and graft polymers PVA-g-P colloidal sol, the pH of mixed liquor is protected
Hold between 7.0~7.5；
S5. the mixed liquor of above-mentioned steps is uniformly coated on eggshell membrane, room temperature is dried, and microorganism modification electricity is made
Pole.
Preferably, the eggshell membrane in the step S5 is treated egg shell inner membrance.
China is that the first egg production and consumption big country, account for freshly-slaughtered poultry egg quality by eggshell 10% calculate in the world, eggshell
Film accounts for eggshell quality 5% and calculated, and it is about 3,400,000 t that China produces eggshell every year, and caused egg shell membrane is about 170,000 t, these discardings
Eggshell and egg shell membrane in resource be the waste of extreme, if egg shell membrane is modified into a kind of biology of biological detection
Film, this will greatly improve the utilization rate of resource, while also reduce the expensive cost of biomembrane, and biological products cost is dropped into one
It is rational horizontal.
Preferably embodiment, the method that eggshell membrane is applied to biomembrane, including following step are will be described in detail below
Suddenly：
S1. the eggshell membrane moisturizing that will be collected into, it is specially that periodically 37 DEG C of deionized waters of spray, subsequent operation process are kept
Eggshell membrane keeps the state of moistening；
S2. lightly hemispherical eggshell membrane is cut along warp, shakeout, i.e., planarized eggshell membrane；
S3. the eggshell membrane of above-mentioned steps is placed in beaker, adds appropriate amount of deionized water, beaker is placed in oscillator
On, 46 DEG C, 100-120rpm, 1-2h is vibrated, after vibration terminates, egg shell membrane is placed in the slow water of flowing and rinsed, removes film
Surface impurity；
S4. the eggshell membrane of above-mentioned steps is placed in plane, eggshell membrane is layered outer membrane and inner membrance using tweezers；
S5. outer membrane is placed in 50mMTris-HCl (pH7.5), in 10mM CaCl2 solution, is placed on magnetic stirring apparatus, incubates
40-55 DEG C, 30min is educated, above-mentioned solution adds 20mg/ml Proteinase K 100ul per 100ml, continues to be incubated 10-20min；
S6. inner membrance is placed in 50mMTris-HCl (pH7.0), in 10mM CaCl2 solution, is placed on magnetic stirring apparatus, incubates
40-55 DEG C, 10min is educated, above-mentioned solution adds 20mg/ml Proteinase K 10ul per 100ml, continues to be incubated 5-10min；
S7. after step S5 and S6 reactions terminate, quickly remove, be placed in the running water of flowing and rinse 3-5min, make afterwards
With 75% alcohol washes 3-5 times, each 5min；
S8. the film of above-mentioned steps is cleaned 3-5 times, each 3min using deionized water, and 4 DEG C after terminating, moisturizing saves backup.
Eggshell membrane is applied in the preparation of sensor by the present invention first, is reduced the preparation cost of sensor, is carried simultaneously
High resource utilization, allow the egg shell usually to go out of use to be recycled utilization, reduce the wasting of resources, it is green.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of scope is protected, although being explained with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent substitution, without departing from the reality of technical solution of the present invention
Matter and scope.
Claims (6)
1. a kind of aquaculture persticide residue early warning system, it is characterised in that including sample introduction module, sample message registration mould
Block, sample pretreatment module, detection module and summary report generation module；The sample introduction module, sample message Registration Module, sample
Product pretreatment module, detection module and summary report generation module electrically connect with central processing unit；The sample introduction module can be with
Receive Feed Sample and animal wastes sample.
2. aquaculture according to claim 1 persticide residue early warning system, it is characterised in that the sample message is stepped on
Remember the information such as the censorship unit of module registers sample, submission date, the form of sample, example weight.
3. aquaculture according to claim 2 persticide residue early warning system, it is characterised in that the sample pretreatment
Module includes disintegrating mechanism, sieving mechanism and dilution module.
4. aquaculture according to claim 3 persticide residue early warning system, it is characterised in that the detection module bag
Include heavy metal analysis module, Detecting Pesticide module and detection of veterinary drugs in food module；The heavy metal analysis module, agricultural chemicals are residual
Staying detection module and detection of veterinary drugs in food module also includes corresponding information acquisition module, and the information transfer collected is to collecting report
Accuse in generation module.
5. aquaculture according to claim 4 persticide residue early warning system, it is characterised in that the residues of pesticides inspection
Survey module includes three-electrode system for a kind of full cell microorganism sensor, including reaction tank and sampling system, the reaction tank,
Cleaning systems and exhaust system；The both sides that reaction tank is incorporated into electrode and reference electrode in the three-electrode system, and with
Pool wall is bonded；The working electrode of three electrode is placed in the centre of reaction tank；The cleaning systems include being arranged on four angles
Shower nozzle and coupled logical cleaning fluid storage room；The exhaust system set with below reaction tank, including work waste liquid and
Clean two outlets of waste liquid；The sensor electrically connects with electrochemical workstation.
6. aquaculture according to claim 5 persticide residue early warning system, it is characterised in that the working electrode
Preparation process, comprise the following steps：
S1. take glass-carbon electrode or platinum electrode to be pre-processed, carry out acid cleaning and alkali cleaning first, finally carry out clear water punching
Wash；
S2. training objective microorganism, objective microbe are Escherichia coli, collect the wet thallus of objective microbe, and 4 degree of preservations are standby
With；
S3. graft polymers PVA-g-P is prepared；
S4. will be sufficiently mixed in the wet thallus of above-mentioned steps and graft polymers PVA-g-P colloidal sol, the pH of mixed liquor is maintained at
Between 7.0~7.5；
S5. the mixed liquor of above-mentioned steps is uniformly coated on eggshell membrane, room temperature is dried, and microorganism modified electrode is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710901973.6A CN107703201A (en) | 2017-09-28 | 2017-09-28 | A kind of aquaculture persticide residue early warning system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710901973.6A CN107703201A (en) | 2017-09-28 | 2017-09-28 | A kind of aquaculture persticide residue early warning system |
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| CN107703201A true CN107703201A (en) | 2018-02-16 |
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| CN201710901973.6A Pending CN107703201A (en) | 2017-09-28 | 2017-09-28 | A kind of aquaculture persticide residue early warning system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108680623A (en) * | 2018-09-05 | 2018-10-19 | 邯郸学院 | A kind of portable electrochemical determining fingerprint pattern instrument |
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