CN107699957A - Fusion based on DNA, which is quantitatively sequenced, builds storehouse, detection method and its application - Google Patents
Fusion based on DNA, which is quantitatively sequenced, builds storehouse, detection method and its application Download PDFInfo
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- CN107699957A CN107699957A CN201710389183.4A CN201710389183A CN107699957A CN 107699957 A CN107699957 A CN 107699957A CN 201710389183 A CN201710389183 A CN 201710389183A CN 107699957 A CN107699957 A CN 107699957A
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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Priority Applications (1)
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CN201710389183.4A CN107699957B (en) | 2017-05-27 | 2017-05-27 | DNA-based fusion gene quantitative sequencing library construction, detection method and application thereof |
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CN201710389183.4A CN107699957B (en) | 2017-05-27 | 2017-05-27 | DNA-based fusion gene quantitative sequencing library construction, detection method and application thereof |
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CN107699957A true CN107699957A (en) | 2018-02-16 |
CN107699957B CN107699957B (en) | 2021-07-23 |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108486235A (en) * | 2018-03-07 | 2018-09-04 | 北京圣谷智汇医学检验所有限公司 | A kind of method and system of high-efficiency and economic detection fusion gene |
CN108866155A (en) * | 2018-06-11 | 2018-11-23 | 中国农业科学院深圳农业基因组研究所 | A kind of preparation method of next generation's sequencing library |
CN110484655A (en) * | 2019-08-30 | 2019-11-22 | 中国医学科学院病原生物学研究所 | The detection method of two generation of parainfluenza virus full-length genome sequencing |
CN110564745A (en) * | 2019-08-27 | 2019-12-13 | 杭州市第一人民医院 | Lung cancer rare ALK fusion mutant gene and detection primer, kit and detection method thereof |
WO2020077400A1 (en) * | 2018-10-17 | 2020-04-23 | PerkinElmer Health Sciences (Australia) Pty Ltd | Barcoding of nucleic acids |
CN112852918A (en) * | 2021-01-20 | 2021-05-28 | 深圳百人科技有限公司 | Two-step PCR technique |
CN114015757A (en) * | 2021-08-31 | 2022-02-08 | 廖端芳 | Method for detecting fusion gene |
WO2022067936A1 (en) * | 2020-09-30 | 2022-04-07 | 厦门飞朔生物技术有限公司 | Method for constructing library for detecting fgfr1/2/3 fusion gene of cholangiocarcinoma on basis of high-throughput sequencing |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010006215A1 (en) * | 2008-07-09 | 2010-01-14 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
CN102628082A (en) * | 2012-04-10 | 2012-08-08 | 凯晶生物科技(苏州)有限公司 | Method for qualitatively and quantitatively detecting nucleic acid based on high-flux sequencing technology |
CN106676182A (en) * | 2017-02-07 | 2017-05-17 | 北京诺禾致源科技股份有限公司 | Low-frequency gene fusion detection method and device |
-
2017
- 2017-05-27 CN CN201710389183.4A patent/CN107699957B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010006215A1 (en) * | 2008-07-09 | 2010-01-14 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
CN102628082A (en) * | 2012-04-10 | 2012-08-08 | 凯晶生物科技(苏州)有限公司 | Method for qualitatively and quantitatively detecting nucleic acid based on high-flux sequencing technology |
CN106676182A (en) * | 2017-02-07 | 2017-05-17 | 北京诺禾致源科技股份有限公司 | Low-frequency gene fusion detection method and device |
Non-Patent Citations (1)
Title |
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ASAO FUJIYAMA ET AL: "Construction and Analysis of a Human-Chimpanzee Comparative Clone Map", 《SCIENCE》 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108486235A (en) * | 2018-03-07 | 2018-09-04 | 北京圣谷智汇医学检验所有限公司 | A kind of method and system of high-efficiency and economic detection fusion gene |
CN108866155A (en) * | 2018-06-11 | 2018-11-23 | 中国农业科学院深圳农业基因组研究所 | A kind of preparation method of next generation's sequencing library |
WO2020077400A1 (en) * | 2018-10-17 | 2020-04-23 | PerkinElmer Health Sciences (Australia) Pty Ltd | Barcoding of nucleic acids |
CN112912514A (en) * | 2018-10-17 | 2021-06-04 | 珀金埃尔默保健科学(澳大利亚)私人有限公司 | Barcoding of nucleic acids |
CN110564745A (en) * | 2019-08-27 | 2019-12-13 | 杭州市第一人民医院 | Lung cancer rare ALK fusion mutant gene and detection primer, kit and detection method thereof |
CN110564745B (en) * | 2019-08-27 | 2021-07-20 | 杭州市第一人民医院 | Lung cancer rare ALK fusion mutant gene and detection primer, kit and detection method thereof |
CN110484655A (en) * | 2019-08-30 | 2019-11-22 | 中国医学科学院病原生物学研究所 | The detection method of two generation of parainfluenza virus full-length genome sequencing |
CN110484655B (en) * | 2019-08-30 | 2021-03-30 | 中国医学科学院病原生物学研究所 | Detection method for parainfluenza virus whole genome second-generation sequencing |
WO2022067936A1 (en) * | 2020-09-30 | 2022-04-07 | 厦门飞朔生物技术有限公司 | Method for constructing library for detecting fgfr1/2/3 fusion gene of cholangiocarcinoma on basis of high-throughput sequencing |
CN112852918A (en) * | 2021-01-20 | 2021-05-28 | 深圳百人科技有限公司 | Two-step PCR technique |
CN114015757A (en) * | 2021-08-31 | 2022-02-08 | 廖端芳 | Method for detecting fusion gene |
CN114015757B (en) * | 2021-08-31 | 2024-02-02 | 江门市灿明生物科技有限公司 | Method for detecting fusion gene |
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