CN107698693A - A kind of fruit polysaccharide discoloration method - Google Patents
A kind of fruit polysaccharide discoloration method Download PDFInfo
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Abstract
The invention discloses a kind of method that fruit polysaccharide crude extract decolourizes.The present invention is surprised to find that there is preferable decolorizing effect to fruit polyoses extract using low concentration electrolyte solution, after being pre-processed to raw material, adds special electrolytic solutions, can pigment in quick, efficient Polysaccharide removing extract.More particularly to using fresh or inferior fruit pulp as raw material, pigment in fruit polyoses extract is removed using low concentration electrolyte solution, includes the setting of the technical parameter such as kind of electrolytes, concentration, pH value, processing time and separation method.The present invention has abandoned the technologies such as the activated carbon used in existing plant polyose extract depigmentation method, resin, hydrogen peroxide, in a creative way using low concentration electrolyte solution processing polyoses extract, obtain percent of decolourization and be more than 90%, polysaccharide recovery is more than 90% decolouring technology, and application prospect is good.
Description
Technical field
The invention belongs to technical field of food deep processing, and in particular to a kind of new work of decolouring for preparing high-purity fruit polysaccharide
Skill.
Background technology
Polysaccharide refers to by the degree of polymerization of glucosides key connection be more than by aldose or ketose 10 macromolecular.With biology skill
The development of art, important function of the polysaccharide in vital movement are sufficiently recognized.Increasing research confirms both at home and abroad,
In addition to the energy substance and cytoskeletal structure as vital movement, polysaccharide and saccharide complex are participating in the various biological phenomenas of cell
Regulation, such as suppress tumour, regulation is immune, reduces blood glucose, antiviral, reducing blood lipid, anticoagulation etc. has high activity.Plant
Glucide, wide material sources are rich in particularly in fruit, and do not have toxic side effect, be prepare new drug, development function health products or
The desirable feedstock of green food additive, while can solve the problems, such as the recycling of cull fruit.The egg contained in polysaccharide crude extract
The impurity such as white matter, pigment can reduce the bioactivity of polysaccharide, influence the exploitation of its high value added product, such as answering for field of medicaments
With.Therefore impurity removal process simple, efficiently, inexpensive is developed, obtains the basis that high-purity polysaccharide is polysaccharide application.
Because the pigment composition in polysaccharide extraction liquid is extremely complex, containing substantial amounts of phytochrome, polysaccharide is both influenceed
Color influence purity again, and the pigment constituent very different in different raw material, or even same raw material difference carry
Taking technique also results in that pigment composition is variant, and therefore, the decolouring of polysaccharide is one of problem maximum in polysaccharide purification research.
The method that the conventional polysaccharide in the country decolourizes has resin adsorption method, ultrasonic extraction, activated carbon method, hydrogen peroxide method
With cetyl trimethylammonium bromide-n-hexyl alcohol-isooctane method.(Polysaccharides from Rosa roxburghii decoloration process research [J] is micro by Chen Muyan etc.
Element and health research, 2012,29 (5):19-20) and Zhang Lihong et al. (research [J] torrid zones of purple perilla leaf polyose decoloration process
Acta Agronomica Sinica, 2014,35 (7):1450-1455) report and remove depigmentation from plant or fruit using resin adsorption method, can
Selectivity adsorpting pigment and can preferably retain the bioactivity of polysaccharide, obtain good effect.However, due to macroporous absorption
Resin price is more expensive, need to pre-process and to regenerate, therefore be unfavorable for large-scale industrial production.
(gingko pollen Thick many candies decoloration process studies [J] Food Sciences, 2009,30 (14) to Hao Gongyuan etc.:136-139.)
Report the decolorizing effect for being better than polyamide, resin and Powdered Activated Carbon using the adsorption effect of granular activated carbon.However, make
Tannin pigment is typically removed with active carbon adsorption.Because of the loose porous non-selectivity of activated carbon, make polysaccharide loss more, therefore shadow
Ring the yield of polysaccharide.
Peng Yongjian, et al. (fragrant solomonseal rhizome polyoses ultrasonic extraction process optimizes and its moisturizing Journal of Sex Research [J] Food Sciences, and 2012,
33(14):The method by ultrasonic wave extraction polysaccharide 96-99.) is reported, its principle is exactly to make using the cavitation of ultrasonic activation
With, mechanism, fuel factor etc. destroy cell membrane, so as to quickly polysaccharide being dissolved in the solvent of pigment indissoluble.This method has
There is equipment to be simple and convenient to operate, efficient energy-saving, avoid long-time and the degraded of high temperature on extraction of substance etc. from influenceing.It is but ultrasonic
The sound frequency that wave frequency rate can be heard close to people, there is certain injury to human body.In addition, the volume and power output of ultrasonic instrument
It is limited, it is difficult to meet the industrial production of scale.
Huang Chun etc. (takes off albumen in Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide extraction and discoloration method compares pharmacy and clinical research, 2007,15 (1):
45-46) it is better than activated carbon method in the decolorizing effect discovery to Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide, hydrogen peroxide method, the concentration of wherein hydrogen peroxide is
Decolorizing effect is best when 5%.However, this method needs strict control decolorization condition, as the concentration of polysaccharide solution, temperature, the time,
PH value etc., the degraded of polysaccharide is otherwise easily caused to be lost, this proposes higher requirement for industrial extensive be applicable.
Fang Jinian et al. reports (Chinese natural drug, 2007,5 (5):A kind of 338-345) new discoloration method, that is, adopt
The inverse micellar solution formed with cetyl trimethylammonium bromide-n-hexyl alcohol-isooctane is thick to polysaccharide under certain salinity
Product are decolourized.This method have the advantages that easily and fast, polysaccharide recovery it is high.However, this method is related to substantial amounts of chemistry examination
Agent, chemical reagent can not be removed completely in the polysaccharide product finally prepared, therefore for being needed in prepared polysaccharide product
Additionally to increase further purification step, therefore add reactions steps.
In the world on pigment removal technical research, report mainly has the technology of microorganism removing coloring agent/pigment at present
(Guangdao Huang,Wei Wang,Guoguang Liu,Simultaneous chromate reduction and azo
dye decolourization by Lactobacillus paracase CL1107isolated from deep sea
Sediment [J] .Journal of Environmental Management, 2015,157 (1)：297-302), enzyme process
(Dalel , Fakher Frikha, Hela Zouari-Mechichi, Lassaad Belbahri, Steve
Woodward, Tahar Mechichi.Application of response surface methodology to
Optimize decolourization of dyes by the laccase-mediator system [J], Journal
Of Environmental Management, 2012,108 (15)：84-91), electrocoagulation (Adhoum, Lotfi
Monser, Decolourization and removal of phenolic compounds from olive mill
Wastewater by electrocoagulation [J], Chemical Engineering and Processing:
Process Intensification, 2004,43 (10)：1281-1287) certain effect, is obtained, but still is existed each
The defects of from present in method.
Therefore, need at present it is a kind of different from above conventional method and it is easy to operate, decolorizing efficiency is high and does not influence more
The discoloration method of sugared product purity.
The content of the invention
It is an object of the invention to provide a kind of method decolourized from fruit polysaccharide crude extract.The present invention is surprised to find that to use
Low concentration electrolyte solution has preferable decolorizing effect to fruit polyoses extract, after being pre-processed to raw material, adds specific
Electrolyte solution, can pigment in quick, efficient Polysaccharide removing extract.More particularly to using fresh or inferior fruit pulp as original
Material, pigment in fruit polyoses extract, including kind of electrolytes, concentration, pH value, processing are removed using low concentration electrolyte solution
The setting of the technical parameter such as time and separation method.
A kind of fruit polysaccharide discoloration method, electrolyte solution progress decolorization is added in fruit polysaccharide slightly carries solution.
The electrolyte solution is selected from FeCl3, FeCl2, AlCl3, CaCl2, ZnCl2, CuCl2, NiSO4, CuSO4,
ZnSO4、MgSO4One or more in solution.
Further preferred, the electrolyte solution is selected from FeCl3、AlCl3、NiSO4、CuSO4In one or more.
Further preferred, the electrolyte solution is selected from FeCl3、CuSO4One or both of.
Further addition electrolyte solution is until 0.0001~0.0500mol/L of final concentration.
Further, fruit polysaccharide is slightly carried into solution and adds electrolyte solution, between adjusting initial pH value 1~13, preferably
2-12；At 0 DEG C~100 DEG C, preferably 40-100 DEG C, after processing, centrifugation pigment.
Further, fruit polysaccharide discoloration method includes any one in the following manner：
1) fruit polysaccharide is slightly carried into solution to be fitted into bag filter, adds distilled water, dialysed at least once, until peritoneal effluent is examined
Do not detect small molecular weight impurity, after the solution in bag filter adds electrolyte treatment, be centrifuged off precipitating, supernatant loads bag filter
Interior, dialysis removes electrolyte；
2) after fruit polysaccharide slightly being carried into solution addition electrolyte solution processing, it is centrifuged off precipitating, supernatant loads dialysis
In bag, dialysis removes electrolyte；
Small molecular weight impurity includes：Molecular weight is less than 2000Da monose, disaccharides, oligosaccharides, pigment, inorganic ions；
After centrifugation depigmentation, supernatant dialysis treatment, the cation concn of the outer solution electrolyte of detection bag filter, when
When not detecting, stop dialysis.Dialysis time is controlled in 0.5-24h.
7-10 times that water consumption is extract solution is distilled in aforesaid way, during dialysis.
Molecule is also used it is preferred that use bag filter of the molecular weight for 2000Da during dialysis, including during except electrolyte cation
Measure the bag filter for 2000Da.
Further preferred, when pigment is removed in korla pear polysaccharides, electrolyte solution is selected from CuSO4Solution, its end
Concentration is 0.006mol/L；Or, selected from FeCl3Solution, its final concentration of 0.0005mol/L.
Further preferred, when pigment is removed in korla pear polysaccharides, electrolyte solution is selected from CuSO4Solution, pH value
2-13, and in 40 DEG C~100 DEG C heating water baths.Then 15-20min is centrifuged with 6000r/min.
Further preferred, when pigment is removed in korla pear polysaccharides, electrolyte solution is selected from FeCl3Solution, pH value
2-12, and in 0 DEG C~100 DEG C heating water baths, 15-20min is then centrifuged with 6000r/min.
The extracting method of the fruit polysaccharide crude extract of the present invention：Fruit is cleaned and removes pericarp, the intact part of selection is beaten
Slurry, filtering, distillation water washing filter residue for several times, quantitatively weigh filter residue, add equivalent distilled water, and pH value at 60-90 DEG C naturally, surpass
Sound (150-500KW) extracts more than 30min, obtains polysaccharide crude extract.
The inventive method is applied to the various fruits such as pears, apple, orange, orange, banana, grape.
The present invention is by taking pigment elimination effect in korla pear polysaccharides as an example, pigment clearance rate such as Fig. 1 of different electrolyte
It is shown.
It will be seen from figure 1 that different electrolyte are FeCl to the pigment clearance rate of korla pear polysaccharides solution3＞
NiSO4＞ CuSO4＞ CaCl2＞ Na2SO4＞ NaCl, wherein FeCl3、NiSO4、CuSO4Pigment clearance rate exceeded 90%,
FeCl3Pigment clearance rate reached 98.6%；By comparing Na2SO4、CuSO4、 NiSO4, it can be found that they have on an equal basis
The SO of concentration4 2-But pigment clearance rate but has larger difference, it is seen that what is really worked is the cation in electrolyte.
It is can be found that by the pigment clearance rate of more different cations：Cationic compound valency is higher, and pigment clearance rate is higher, same to valence state
The pigment clearance rate of cation is close, and this is consistent with negatively charged sol property.
CuSO4、FeCl3Influence of the dosage to Kuerle delicious pear pigment clearance rate is as shown in Figure 2.Configuration concentration is
0.05mol/L CuSO4、0.002mol/L FeCl3, accurately pipette dialysis and remove the korla pear polysaccharides solution after oligosaccharides
1.0mL, add the FeCl that different volumes have configured3、CuSO4Solution, moisturizing to 10.0mL, 60 DEG C of heating water bath 30min, from
The heart, the absorbance at supernatant measure 420nm is taken, and calculate pigment clearance rate.To add FeCl3、CuSO4The volume of solution is
Abscissa, pigment clearance rate are that ordinate makees Fig. 2.Work as Cu2+Concentration reaches about 0.005mol/L, and the increase of pigment clearance rate starts
Slow down, Fe3+Concentration reaches about 0.0004mol/L, and the increase of pigment clearance rate starts to slow down.Figure it is seen that pigment is clear
Except rate is with CuSO4And FeCl3Dosage increase and increase.CuSO4With FeCl3Pigment clearance rate it is close when, CuSO4Concentration
For FeCl325 times of concentration.This phenomenon is consistent with Schulze-Ha Di rule of valence, i.e. coagulation value and the six of counter ion valence mumber
Power is inversely proportional, thus it is speculated that it is that colloidal coagulation is similar to remove mechanism.
By taking korla pear polysaccharides depigmentation as an example, the pigment clearance rate under different pH value is as shown in Figure 3.Can from figure
Go out, between pH value 2~13, CuSO4Pigment clearance rate be slowly increased with the increase of pH value, reach most in pH value 13
Greatly, but to being minimized on the contrary during pH value 14, thus it is speculated that it is cation to remove work to pigment, and as pH ＜ 7, pH value is got over
It is low, Cl in solution-More (testing with salt acid for adjusting pH), Cl-To Cu2+Parcel is formed, so as to reduce pigment clearance rate, pH
Value 1 is more slightly higher than the pigment clearance rate of pH value 2, and this is probably due to H+Belong to cation, there is certain pigment Scavenging activity；When
During pH ＞ 7, part Cu2+Meeting and OH-Reaction generation Cu (OH)2(25 DEG C, Ksp=2.2 × 10-20) flocculent deposit, these are cotton-shaped heavy
Form sediment may adsorpting pigment, pigment clearance rate now is by Cu2+With Cu (OH)2The coefficient result of flocculent deposit, with
The increase of pH value, pigment clearance rate are also slowly increased, but when pH value 14, a large amount of Cu2+With OH-Reaction generation Cu (OH)2It is cotton-shaped
Precipitate, Cu in solution2+Concentration drastically reduces, and has then resulted in drastically reducing for pigment clearance rate.
FeCl3Pigment clearance rate change in pH ＜ 7, with CuSO4Identical, reason is consistent, but in pH ＞ 7, FeCl3
Pigment clearance rate reduced with the increase of pH value, and 0 is fallen below in pH value 14, this is due to FeCl3Concentration only have
CuSO41/25, in alkaline environment, Fe3+Meeting and OH-Reaction generation Fe (OH)3(25 DEG C, Ksp=8.0 × 10-38) precipitation, arrive
During pH value 13, Fe in solution3+Content is drastically reduced, so see that pigment clearance rate substantially reduces, during to pH value 14, and solution
In almost without Fe3+, then pigment clearance rate be reduced to 0.Acid or alkaline environment is likely to cause Kuerle delicious pear more
The degraded of sugar or activity change.
By taking korla pear polysaccharides depigmentation as an example, influence of the bath temperature to pigment clearance rate is as shown in Figure 4.From Fig. 4
As can be seen that CuSO4Pigment clearance rate increase with the increase of bath temperature, FeCl3Pigment clearance rate hardly by temperature
Influence, the pigment clearance rate both when 80 DEG C is very close, when bath temperature is more than 80 DEG C, CuSO4Pigment it is clear
Except a little higher than FeCl of rate3。
The technology of the present invention effect：
1. the present invention has abandoned activated carbon, resin, the hydrogen peroxide used in existing plant polyose extract depigmentation method
Etc. technology, polyoses extract is handled using low concentration electrolyte solution in a creative way.
2. the inventive method has easy to operate, speed fast and is not introduced into any poisonous or difficult relative to conventional method
The quality of food-grade or medical grade can be reached with the organic reagent or chemical reagent of removing, therefore in prepared polyose
Standard.
3. the inventive method is can to obtain percent of decolourization more than 90%, polysaccharide recovery is more than 90% decolouring technology.
4. the method for the present invention solves the problems, such as the recycling of inferior fruit, the raw material sources of plant polyose are widened, greatly
Improve the utilization rate of cull fruit resource and solve its pollution problem to environment in ground.
5. the present invention can effectively improve the added value of fruit, and be by the deep processing to fruit, particularly inferior fruit
Plant polyose, the Research Thinking new except color offer of even other extracts and technology.
Brief description of the drawings
Fig. 1 is the pigment clearance rate of different electrolyte；
Fig. 2 is CuSO4,FeCl3Influence of the dosage to pigment clearance rate；
Fig. 3 is influence of the pH value to pigment clearance rate；
Fig. 4 is influence of the temperature to pigment clearance rate；
Fig. 5 is influence of the different dialysis steps to pigment clearance rate.
Embodiment
Below, the present invention will be further detailed with embodiment, but it is not limited to any of these embodiments
Individual or similar example.
Embodiment 1：Utilize CuSO4Solution is decolourized from the polysaccharide crude extract of Kuerle delicious pear
The first step：Inferior Kuerle delicious pear is cleaned into peeling, intact part is chosen and is beaten, is filtered, distillation washing
Wash filter residue for several times, quantitatively weigh filter residue, add equivalent distilled water, pH value is naturally, ultrasonic (150-500KW) is carried at 60-90 DEG C
30min is taken, obtains polysaccharide crude extract.
Second step：By polysaccharide crude extract, load 2000Da bag filter, dialysed with 7 times of distilled water, changed before dialysis equilibrium
Water, small molecular weight impurity of the molecular weight less than 2000Da is can't detect to peritoneal effluent (main component is monose, disaccharides and oligosaccharides).
3rd step：By polysaccharide solution in bag filter, the CuSO prepared is added4Solution, CuSO4Final concentration in the solution
For 0.006mol/L, pH value 7, heating water bath, temperature 60 C, after heating 30min, 4000r/min, 5-15min is centrifuged, removed
Pigment.
4th step：Supernatant loads in bag filter, at least dialyses once, removes electrolyte.
Embodiment 2：Decolourized from the polysaccharide crude extract of pyrus nivalis
Step 1-2 is carried out according to the method for embodiment 1.
3rd step：By polysaccharide solution in bag filter, the CuSO prepared is added4Solution, CuSO4Final concentration in the solution
For 0.006mol/L, pH value 7, heating water bath, temperature 60 C, after heating 30min, 4000r/min, 5-15min is centrifuged, removed
Pigment.
4th step：Supernatant loads in bag filter, at least dialyses once, removes electrolyte.
Embodiment 3：Decolourized from the polysaccharide crude extract of apple
Step 1-2 is carried out according to the method for embodiment 1.
3rd step：By polysaccharide solution in bag filter, the FeCl prepared is added3Solution, FeCl3Final concentration in the solution
For 0.0005mol/L, pH value temperature 60 C, after heating 30min, 6000r/min centrifugation 20min, removes naturally, heating water bath
Pigment.
4th step：Supernatant loads in bag filter, at least dialyses once, removes electrolyte.
Embodiment 4：Decolourized from the polysaccharide crude extract of Peel of Navel Orange
Step 1-2 is carried out according to the method for embodiment 1.
3rd step：By polysaccharide solution in bag filter, add the calcium chloride solution or nickel sulfate solution prepared, calcium chloride and
The final concentration of nickel sulfate in the solution is 0.0167mol/L, and pH value temperature 60 C, heats 30min naturally, heating water bath
Afterwards, 6000r/min centrifuges 20min, except depigmentaton.
4th step：Supernatant loads in bag filter, at least dialyses once, removes electrolyte.
Pigment content in embodiment 5, polysaccharide extraction liquid
Step 1-2 is carried out according to embodiment 1-4 methods, dialysis is accurately pipetted and removes solution after small molecular weight impurity
10.00mL, carried out by embodiment 1-4 step 3, while corresponding electrolyte solution 2.0mL is added by step with 10.00mL distilled water
Rapid 3 are carried out, as reference solution；Step 3 centrifuged supernatant determines absorbance under 420nm, calculates pigment removal rate (result
It see the table below 1).
Pigment removal rate=(A420Before processing-A420After processing)/A420Before processing× 100%
In formula, A420Before processing、A420After processingBefore referring to electrolyte treatment respectively, after processing solution absorbance.
Table 1
| Pigment content | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Before decolouring | 0.596 | 0.836 | 0.380 | 0.466 |
| After decolouring | 0.0597 | 0.065 | 0.052 | 0.049 |
| Percent of decolourization | 89.97% | 92.24% | 86.31% | 89.48% |
Polysaccharide recovery in embodiment 6, polysaccharide extraction liquid
The first step：The making of glucose standard curve
Precision weighs to be dried to the dextrose standard sample 0.0235g of constant weight at 105 DEG C, and distilled water is settled to 100.0mL appearances
In measuring bottle, the dextrose standard sample mother liquor that concentration is 0.235mg/mL is obtained.
Dextrose standard sample mother liquor 2.00mL is taken, 2.00mL distilled water is added, is configured to glucose sample liquid.Take successively
0.00mL, 0.20mL, 0.40mL, 0.60mL, 0.80mL, 1.00mL glucose sample liquid are in 1,2,3,4,5, No. 6 test tube,
Then moisturizing adds the phenol of 1.00mL 5%, the 5.00mL concentrated sulfuric acids, No. 4 test tubes is taken after cooling in 200~800nm to 2.00mL
Between carry out spectral scan, determine maximum absorption wavelength 489nm, absorbance determined under maximum absorption wavelength, and with polyoses content
For abscissa, absorbance is that ordinate makes glucose standard curve.
Second step：Phenol-dense sulphuric acid method absorbance study on the stability
To determine that phenol-dense sulphuric acid method detects the optimum reacting time of polysaccharide, design stability experiment is as follows：
The accurate polysaccharide extraction liquid 2.00mL that pipettes adds the phenol of 1.00mL 5%, the 5.00mL concentrated sulfuric acids, ultraviolet-visible point
Absorbance at light photometric determination 489nm, instrument setting are repeated to read once, read 10 times altogether every 5min.Using the time as horizontal stroke
Coordinate, absorbance are that ordinate makes absorbance stability curve, and determining that solution absorbance measures in 10-50min is advisable.
3rd step：Step 1-2 is carried out according to embodiment 1-4 methods, dialysis is accurately pipetted and removes solution after small molecular weight impurity
10.00mL, carried out by embodiment 1-4 step 3, the accurate centrifuged supernatant 2.00mL of removing step 3, add 1.00mL
5% phenol, the 5.00mL concentrated sulfuric acids, absorbance is determined under 489nm after cooling, corresponding electrolyte is added with 10.00mL distilled water
Solution 2.0mL (as reference solution), calculate polysaccharide recovery (result see the table below 2).
Polysaccharide recovery=A489After processing/A489Before processing× 100%
In formula, A489Before processing、A489After processingBefore referring to electrolyte treatment respectively, after processing solution absorbance.
Table 2
| Polyoses content | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Before decolouring | 0.502 | 0.644 | 0.522 | 0.452 |
| After decolouring | 0.456 | 0.537 | 0.419 | 0.329 |
| The rate of recovery | 90.83% | 83.38% | 80.27% | 72.78% |
Embodiment 7：By taking korla pear polysaccharides decolorizing effect as an example：Shadow of the difference dialysis step to electrolyte depigmentation
Ring：
A groups：Accurately pipette korla pear polysaccharides concentrate 100.0mL, be respectively charged into molecular weight for 2000Da,
In 3500Da, 5000Da bag filter, after dialysis removes small molecular weight impurity (main component is monose, disaccharides and oligosaccharides), point
Jia Ru not 15.00mL 0.50mol/L CuSO4Solution, heating water bath 30min, it is centrifuged off precipitating, supernatant is respectively charged into
Analyse in bag, water, the outer solution C u of Pulse sample introduction device detection bag filter are changed in interval2+Concentration, stop dialysis, constant volume when not detecting
Into 100.0mL volumetric flasks, the absorbance at solution measure 420nm after constant volume is taken.
B groups：Korla pear polysaccharides concentrate 100.0mL accurately is pipetted, is separately added into 15.00mL 0.50mol/L
CuSO4, heating water bath 30min, it is centrifuged off precipitating, supernatant is respectively charged into bag filter, and water, Atomic absorption luminosity are changed in interval
The outer solution C u of method detection bag filter2+Concentration, stop dialysis when not detecting, be settled in 100.0mL volumetric flasks, take molten after constant volume
Absorbance at liquid measure 420nm.
From fig. 5, it can be seen that the absorbance of A groups is significantly lower than B groups, illustrate that electrolyte ion is (main with small molecular weight impurity
It is monose, disaccharides and oligosaccharides to want composition) when dialysing together, pigment elimination effect can be reduced.Therefore, first dialysis removes small molecule
Impurity (main component is monose, disaccharides and oligosaccharides), electrolyte decolouring is reused, there is more preferable decolorizing effect.After decolourizing
The concentration of korla pear polysaccharides solution, obtain decolouring korla pear polysaccharides DPBP after vacuum freeze drying.
Claims (10)
1. a kind of fruit polysaccharide discoloration method, it is characterised in that add electrolyte solution in fruit polysaccharide slightly carries solution and carry out
Decolorization.
2. fruit polysaccharide discoloration method according to claim 1, it is characterised in that the electrolyte solution is selected from FeCl3,
AlCl3, FeCl2, CaCl2, ZnCl2, CuCl2, NiSO4, CuSO4, ZnSO4、MgSO4One or more in solution.
3. fruit polysaccharide discoloration method according to claim 2, it is characterised in that the electrolyte solution is selected from FeCl3、
AlCl3、NiSO4、CuSO4、CaCl2In one or more.
4. fruit polysaccharide discoloration method according to claim 3, it is characterised in that the electrolyte solution is selected from FeCl3、
CuSO4One or both of.
5. the fruit polysaccharide discoloration method according to claim 1 or 2 or 3 or 4, it is characterised in that addition electrolyte solution
To 0.0001~0.0500mol/L of final concentration.
6. the fruit polysaccharide discoloration method according to claim 1 or 2 or 3 or 4, it is characterised in that slightly carry fruit polysaccharide
Solution adds electrolyte solution, between adjusting initial pH value 1~13, after 0 DEG C~100 DEG C processing, and centrifugation pigment.
7. the fruit polysaccharide discoloration method according to claim 1 or 2 or 3 or 4, it is characterised in that including in the following manner
Any one：
1) fruit polysaccharide is slightly carried into solution to be fitted into bag filter, adds distilled water, dialysed at least once, until peritoneal effluent detection is not
To small molecular weight impurity, after the solution in bag filter adds electrolyte treatment, it is centrifuged off precipitating, supernatant loads in bag filter,
Dialysis removes electrolyte；
2) after fruit polysaccharide slightly being carried into solution addition electrolyte solution processing, it is centrifuged off precipitating, supernatant loads in bag filter,
Dialysis removes electrolyte.
8. fruit polysaccharide discoloration method according to claim 7, it is characterised in that
Small molecular weight impurity includes：Molecular weight is less than 2000Da monose, disaccharides, oligosaccharides, pigment, inorganic ions；
After centrifugation depigmentation, supernatant dialysis treatment, the cation concn of the outer solution electrolyte of detection bag filter, when not examining
When going out, stop dialysis.
9. fruit polysaccharide discoloration method according to claim 6, it is characterised in that
Electrolyte solution is selected from CuSO4Solution, its final concentration of 0.006mol/L, or selected from FeCl3Solution, its is final concentration of
0.0005mol/L。
10. fruit polysaccharide discoloration method according to claim 6, it is characterised in that
Electrolyte solution is selected from CuSO4Solution, pH value 2-13, and in 40 DEG C~100 DEG C heating water baths；
Electrolyte solution is selected from FeCl3Solution, pH value 2-12, and in 0 DEG C~100 DEG C heating water baths.
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| CN109021131A (en) * | 2018-07-13 | 2018-12-18 | 仲恺农业工程学院 | Separation and purification method for plant polysaccharide |
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| CN107698693B (en) | 2020-02-18 |
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