CN107693607B - Traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete involution of uterus and preparation method thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete involution of uterus and preparation method thereof Download PDFInfo
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- CN107693607B CN107693607B CN201711086851.2A CN201711086851A CN107693607B CN 107693607 B CN107693607 B CN 107693607B CN 201711086851 A CN201711086851 A CN 201711086851A CN 107693607 B CN107693607 B CN 107693607B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/754—Evodia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention provides a traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete uterine involution and a preparation method thereof, wherein the traditional Chinese medicine composition comprises, by weight, 20-50 parts of motherwort, 10-30 parts of leech powder, 10-30 parts of fructus evodiae and 15-25 parts of red date. The preparation method of the composition comprises the following steps: (1) weighing motherwort, evodia rutaecarpa and red dates according to the parts by weight, uniformly mixing, adding water in an amount which is 4 times of the total weight of the motherwort, the evodia rutaecarpa and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the evodia rutaecarpa and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating; (2) weighing leech powder according to the weight part, crushing, sieving by a 200-mesh sieve, adding into the concentrated solution in the step (1), and uniformly mixing. Compared with the prior art, the traditional Chinese medicine composition has the advantages of simple formula, simple preparation process, low cost, obvious treatment effect on retained afterbirth and incomplete uterine involution, and great promotion of the recovery of postpartum reproductive function of cows.
Description
Technical Field
The invention relates to a veterinary drug composition, in particular to a traditional Chinese medicine composition and a preparation method thereof, and especially relates to a traditional Chinese medicine composition for preventing and treating retention of placenta and incomplete involution of uterus of cows and a preparation method thereof.
Background
After the cow parturites, the most frequently occurring diseases are retained afterbirth, the incidence rate is as high as 5% -28%, and further the involution of uterus is influenced, if the cow can not be timely and effectively treated, the cow can be easily caused to continuously have no oestrus and infertility, the postpartum reproductive performance is greatly reduced, and finally the development of the cattle raising industry is influenced.
At present, the traditional Chinese medicine motherwort is used for treating postpartum diseases of cows more generally, but the treatment effect of only using a single medicine motherwort is poorer, so researchers usually use other traditional Chinese medicines for promoting blood circulation to remove blood stasis and promoting qi circulation to promote diuresis to improve the curative effect, more commonly use the classical famous prescription biochemical decoction, the traditional Chinese medicine motherwort is composed of angelica, ligusticum wallichii, peach kernel, baked ginger and liquorice, has the effects of warming channel and dispelling cold and promoting blood circulation to remove blood stasis, and the medicine taste is increased and decreased on the basis, for example, CN101757157A uses the angelica, the ligusticum wallichii, peach kernel, the motherwort, the red peony root, the argy wormwood leaf, the codonopsis pilosula and the astragalus root as the prescription; CN102895646A radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, semen Persicae, rhizoma Zingiberis recens, radix astragali, herba Leonuri, semen plantaginis, Glycyrrhrizae radix, Poria, fructus Aurantii, and brown sugar are used for treating retained afterbirth of breeding cow. In order to ensure the postpartum recovery of cows, the formula contains a large amount of medicines for promoting blood circulation to remove blood stasis and medicines for tonifying qi, warming meridians, removing blood stasis and relieving pain, so that the components of the whole medicinal materials are very complex, some formula even contains more than twenty medicinal materials, and the dosage of the medicinal materials is large, so that the preparation process of the medicine is complicated, the price is high, harmful impurities containing toxic and side effects are possibly increased, the medication safety is influenced, and the cow milk powder has potential risk on safe eating of milk. Therefore, the research on the traditional Chinese medicine composition which has simpler formula, more convenient preparation and better safety and is used for preventing and treating retained afterbirth and incomplete involution of uterus and ensuring the postpartum recovery of the cow is very meaningful.
Disclosure of Invention
The invention provides a traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete uterus involution and a preparation method thereof.
The technical scheme of the invention is as follows: a traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete involution of uterus comprises the following components in parts by weight: 20-50 parts of motherwort, 10-30 parts of leech powder, 10-30 parts of fructus evodiae and 15-25 parts of red dates.
Preferably, the traditional Chinese medicine composition consists of the following components in parts by weight: 30-45 parts of motherwort, 15-20 parts of leech powder, 10-20 parts of fructus evodiae and 15-20 parts of red dates.
More preferably, the traditional Chinese medicine composition consists of the following components in parts by weight: 40 parts of motherwort, 20 parts of leech powder, 20 parts of fructus evodiae and 15 parts of red date.
The invention also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
(1) weighing motherwort, evodia rutaecarpa and red dates according to the parts by weight, uniformly mixing, adding water in an amount which is 4 times of the total weight of the motherwort, the evodia rutaecarpa and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the evodia rutaecarpa and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating;
(2) weighing leech powder according to the weight part, crushing, sieving by a 200-mesh sieve, adding into the concentrated solution in the step (1), and uniformly mixing.
The core medicines of the traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete uterine involution are motherwort herb, leech powder, evodia rutaecarpa and red date, wherein:
(1) the motherwort can strengthen the contraction of the uterine smooth muscle, promote the elimination of foreign matters in the uterus, has the bacteriostatic action and can effectively relieve infection;
(2) the leech powder has the anticoagulation effect, can dissipate blood stasis in an invisible way without hurting the qi system, promotes blood circulation to remove blood stasis, dredges meridians and removes food retention, and has a synergistic effect with the motherwort;
(3) fructus evodiae has effects of invigorating stomach, relieving pain, resisting thrombosis, resisting bacteria and virus, and can be used for treating abdominal pain during menstruation and cold hernia abdominal pain;
(4) the red dates have the effects of tonifying deficiency and qi, nourishing blood and soothing nerves, strengthening spleen and stomach, improving body functions and enhancing immunity.
Although the whole formula is simple and the medicines have few medicines, the traditional Chinese medicine composition has the effects of tonifying qi and activating blood, warming meridians and relieving pain, tonifying deficiency and tonifying qi due to scientific and reasonable compatibility, effectively utilizes the synergistic effect among medicines, has extremely obvious treatment effect on retained afterbirth and incomplete uterine involution, can effectively promote the recovery of postpartum reproductive function of cows, does not have drug resistance, and does not leave substances harmful to human bodies in milk. Meanwhile, the medicines are common traditional Chinese medicines, the preparation process is simple, the cost of the medicinal materials is low, and the product stability is good.
Detailed Description
The present invention will be further described with reference to the following examples.
Comparative example 1
Cleaning herba Leonuri 40g, ultrasonic extracting with 80% ethanol for 30min, filtering, recovering ethanol from filtrate, and concentrating to obtain herba Leonuri paste; adding 1 time of water into 100g of brown sugar, heating to 100 ℃ for dissolving, adding the motherwort paste, stirring for dissolving, and concentrating under reduced pressure at 70 ℃ to obtain a motherwort brown sugar concentrated solution.
Comparative example 2
The formula and the preparation method according to embodiment 1 of CN101757157A are as follows: weighing 30g of angelica, 20g of ligusticum wallichii, 25g of peach kernel, 30g of motherwort, 10g of red paeony root, 20g of folium artemisiae argyi, 15g of codonopsis pilosula and 15g of astragalus mongholicus, crushing the traditional Chinese medicines, sieving by a 200-mesh sieve, and uniformly mixing. Adding 1 time of water into 100g of brown sugar, heating to 100 deg.C for dissolving, adding the above Chinese medicinal powder, stirring, and concentrating under reduced pressure at 70 deg.C to obtain concentrated solution.
Comparative example 3
According to the method of CN102895646A embodiment, 55g of angelica, 40g of ligusticum wallichii, 40g of peach kernel, 80g of ginger, 50g of astragalus mongholicus, 120g of motherwort, 100g of plantain seed, 20g of liquorice, 30g of poria cocos, 30g of fructus aurantii and 200g of brown sugar are weighed, ground into powder and brewed with boiling water.
Comparative example 4
Weighing 80g of motherwort and 30g of leech powder, uniformly mixing the motherwort and the leech powder, adding 3 times of water, extracting, and concentrating the water extract.
Comparative example 5
Weighing 60g of motherwort and 20g of leech powder, uniformly mixing the motherwort and the leech powder, adding 3 times of 60% ethanol, extracting, concentrating the ethanol extract into paste, adding 1 time of water into 100g of brown sugar, heating to 100 ℃ for dissolving, adding the ointment, stirring for dissolving, and concentrating under reduced pressure at 70 ℃ to obtain concentrated solution.
Comparative example 6
Weighing 40g of motherwort, 20g of leech powder and 10g of red dates, uniformly mixing the motherwort and the red dates, adding 3 times of water, extracting, concentrating the water extract, crushing the leech powder, sieving with a 200-mesh sieve, adding the crushed leech powder into the concentrated solution, and uniformly mixing.
Example 1
Weighing 20g of motherwort, 30g of leech powder, 30g of fructus evodiae and 25g of red dates, uniformly mixing the motherwort, the fructus evodiae and the red dates, adding water in an amount which is 4 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating; pulverizing Hirudo powder, sieving with 200 mesh sieve, adding into the above concentrated solution, and mixing.
Example 2
Weighing 50g of motherwort, 10g of leech powder, 10g of fructus evodiae and 15g of red dates, uniformly mixing the motherwort, the fructus evodiae and the red dates, adding water in an amount which is 4 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating; pulverizing Hirudo powder, sieving with 200 mesh sieve, adding into the above concentrated solution, and mixing.
Example 3
Weighing 30g of motherwort, 20g of leech powder, 20g of fructus evodiae and 20g of red date, uniformly mixing the motherwort, the fructus evodiae and the red date, adding water in an amount which is 4 times of the total weight of the motherwort, the fructus evodiae and the red date, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the fructus evodiae and the red date, extracting for 1 time, combining water extracts of the two times, and concentrating; pulverizing Hirudo powder, sieving with 200 mesh sieve, adding into the above concentrated solution, and mixing.
Example 4
Weighing 45g of motherwort, 15g of leech powder, 10g of fructus evodiae and 15g of red dates, uniformly mixing the motherwort, the fructus evodiae and the red dates, adding water in an amount which is 4 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating; pulverizing Hirudo powder, sieving with 200 mesh sieve, adding into the above concentrated solution, and mixing.
Example 5
Weighing 40g of motherwort, 20g of leech powder, 20g of fructus evodiae and 15g of red dates, uniformly mixing the motherwort, the fructus evodiae and the red dates, adding water in an amount which is 4 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the fructus evodiae and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating; pulverizing Hirudo powder, sieving with 200 mesh sieve, adding into the above concentrated solution, and mixing.
Clinical test material
1. Test drug
Comparative examples 1 to 6 and examples 1 to 5 of the Chinese medicinal composition of the present invention.
2. Inclusion criteria for test animals
The study is carried out in a certain dairy farm in Sichuan province from 2016 to 2017 in 5 months, healthy cows and calves are selected as the study subjects of the study, the selected cows are healthy in pregnancy, self-parturition, and have no complications or complications before and after delivery, and the calves are healthy, have 2-5 births and have the body condition score of 3.0-4.0 (Edmonson, 1989).
3. Test animal exclusion criteria
During the test period, if the tested cows receive anti-inflammatory or antibiotic treatment measures irrelevant to the study or clinical mastitis, hoof diseases, accidental death and the like occur, the failure to complete the established administration scheme and the calving cows with postpartum hemorrhage or bleeding tendency should be excluded from the final result analysis.
4. Test procedure
Calving cows in accordance with the present study were randomly divided into 12 groups of 100 cows, and 1-11 groups were administered with the concentrated solutions of comparative examples 1-6 and examples 1-5 within 2 hours after delivery, each daily oral drench-administration was performed 1 time, each 400g, for 3 consecutive days, and 12 th group was blank group, no administration was performed. Subsequently, the test cows were monitored and recorded for the discharge of the placenta, and manual stripping of the retained placenta was strictly prohibited throughout the trial. And recording the uterine state and the discharge condition of the fetal membranes 14 days after delivery, wherein the vaginal examination finds that part of the fetal membranes are retained in the vagina, the cervix is closed, the fetal membranes can be diagnosed to be not dropped by two fingers, the rectal examination finds that the uterus still has lochia or purulent secretion discharge, the uterus is large or the uterine wall is swollen, the uterus is poor in recovery, slow and contains a large amount of liquid, and the massage on the uterus, the cervix and the front part of the vagina can promote the outflow of the malodorous secretion, so that the uterine involution is determined to be incomplete. And the reproductive performance of the tested cows was recorded within 180 days of delivery.
5. Test results
The incidence of retained afterbirth, incomplete involution of uterus and pregnancy rate within 180 days of the 14 th day group of cows are shown in the following table.
| Test examples | Incidence of retained afterbirth in 14 days | Incidence of incomplete involution of uterus in 14 days | Pregnancy rate within 180 days |
| Comparative example 1 | 20.4％ | 17.5％ | 40.3％ |
| Comparative example 2 | 15.9％ | 14.2％ | 44.4％ |
| Comparative example 3 | 13.3％ | 15.5％ | 47.3％ |
| Comparative example 4 | 12.6％ | 13.3％ | 49.2％ |
| Comparative example 5 | 11.2％ | 12.7％ | 55.7％ |
| Comparative example 6 | 10.7％ | 10.3％ | 52.5％ |
| Example 1 | 5.2％ | 4.6％ | 75.2％ |
| Example 2 | 5.0％ | 5.2％ | 77.3％ |
| Example 3 | 4.6％ | 4.8％ | 76.5％ |
| Example 4 | 4.0％ | 3.7％ | 82.4％ |
| Example 5 | 3.5％ | 4.2％ | 87.0％ |
| Blank group | 27.5％ | 43.2％ | 35.6％ |
The test results show that compared with the blank group and the groups of comparative examples 1-6, the retention rate of placenta after 14 days and the occurrence rate of incomplete uterine involution are both obviously reduced, and the pregnancy rate of the cow within 180 days is obviously improved, which shows that compared with the medicaments of the comparative examples, the traditional Chinese medicine composition has very positive and effective treatment effects on the prevention and treatment of retention of placenta after delivery and incomplete uterine involution of the cow, has scientific formula compatibility and obvious curative effect, and greatly promotes the recovery of the reproductive function of the cow after delivery.
Claims (2)
1. A traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete involution of uterus is characterized by comprising the following components in parts by weight: 30-45 parts of motherwort, 15-20 parts of leech powder, 10-20 parts of fructus evodiae and 15-20 parts of red dates;
and, a method for preparing the composition, comprising the steps of:
(1) weighing motherwort, evodia rutaecarpa and red dates according to the parts by weight, uniformly mixing, adding water in an amount which is 4 times of the total weight of the motherwort, the evodia rutaecarpa and the red dates, extracting for 1 time, then adding water in an amount which is 2 times of the total weight of the motherwort, the evodia rutaecarpa and the red dates, extracting for 1 time, combining water extracts of the two times, and concentrating;
(2) weighing leech powder according to the weight part, crushing, sieving by a 200-mesh sieve, adding into the concentrated solution in the step (1), and uniformly mixing.
2. The traditional Chinese medicine composition for preventing and treating cow retained afterbirth and incomplete uterine involution as claimed in claim 1, which is characterized by comprising the following components in parts by weight: 40 parts of motherwort, 20 parts of leech powder, 20 parts of fructus evodiae and 15 parts of red date.
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| CN114848802B (en) * | 2022-05-23 | 2023-05-09 | 黑龙江八一农垦大学 | Bioactive composition for preventing and/or treating retention of cow placenta, application thereof, powder and tablet |
| CN116350737B (en) * | 2023-02-27 | 2023-09-08 | 黑龙江八一农垦大学 | Composition for preventing cow fetal membranes from being damaged and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653476A (en) * | 2008-08-22 | 2010-02-24 | 天津生机集团股份有限公司 | Chinese veterinary medicament composition for treating dairy cow retained afterbirth and preparation method thereof |
| CN104352703A (en) * | 2014-10-13 | 2015-02-18 | 天津瑞贝特科技发展有限公司 | Medicine composition for treating retained fetal membranes of milk cow and preparation method of medicine composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653476A (en) * | 2008-08-22 | 2010-02-24 | 天津生机集团股份有限公司 | Chinese veterinary medicament composition for treating dairy cow retained afterbirth and preparation method thereof |
| CN104352703A (en) * | 2014-10-13 | 2015-02-18 | 天津瑞贝特科技发展有限公司 | Medicine composition for treating retained fetal membranes of milk cow and preparation method of medicine composition |
Non-Patent Citations (1)
| Title |
|---|
| 益母草在防治奶牛胎衣不下中的妙用;薛彦安;《中兽医学杂志》;20071231(第02期);46 * |
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