CN107688097B - A kind of method and device being loaded tracer - Google Patents
A kind of method and device being loaded tracer Download PDFInfo
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- CN107688097B CN107688097B CN201710721859.5A CN201710721859A CN107688097B CN 107688097 B CN107688097 B CN 107688097B CN 201710721859 A CN201710721859 A CN 201710721859A CN 107688097 B CN107688097 B CN 107688097B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G08—SIGNALLING
- G08C—TRANSMISSION SYSTEMS FOR MEASURED VALUES, CONTROL OR SIMILAR SIGNALS
- G08C17/00—Arrangements for transmitting signals characterised by the use of a wireless electrical link
- G08C17/02—Arrangements for transmitting signals characterised by the use of a wireless electrical link using a radio link
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Abstract
The present invention provides a kind of methods for being loaded tracer, preset pipettor path, and action message collector acquires the relevant action message of pipettor liquid relief；Action message collector judges that pipettor is in imbibition state or drain state；After pipettor enters drain state from imbibition state, action message collector notifies tracer platform, and control cue module is shown, indicates that current plate hole complete by liquid relief.Invention also provides a kind of sample-adding tracers, including by the first power module, action message acquisition sensor module, action data collector module, data filtering module, the sample-adding information acquisition device that CPU module and first communication module form and the sample-adding tracer platform being made of second power supply module, MCU module, cue module and second communication module.The present invention can prevent experiment operator from multiple drain or missing drain to some plate hole in a plate hole, and structure is simple, easy to operate.
Description
Technical field
The present invention relates to a kind of method and apparatus for being loaded tracer, belong to biochemical analysis method and equipment technology field.
Background technique
Liquid relief, sample-adding are required in hospital, biochemical R&D institution, the biochemical investigation of biochemical production unit, chemical examination, experiment
Operation.Existing artificial pipettor and microwell plate can not all indicate the plate hole of drain and i.e. by the plate holes of drain.Operation
The mechanical labour of personnel's repeatability is very easy to cause in a plate hole multiple drain or misses the drain to some plate hole, thus
Influence the result of biochemical investigation, chemical examination, experiment.
Summary of the invention
In order to solve the deficiencies in the prior art, the present invention provides a kind of method and apparatus for being loaded tracer, can indicate
The plate hole of drain and i.e. by the plate hole of drain out prevents experiment operator from multiple drain or missing pair in a plate hole
The drain of some plate hole, and structure is simple, it is easy to operate.
The present invention is that technical solution used by solving its technical problem is: providing a kind of method for being loaded tracer, packet
Include following steps:
The sample-adding path of S1, default pipettor, the sample-adding carried out for pipettor for each plate hole are operated, are repeated respectively
Step S2 to S11；
S2, using action message acquisition sensor module perception pipettor button since being pressed, process first is stopped
Point, last button are released the process between Aligning control, obtain the imbibition process data of pipettor；
S3, the imbibition of action message acquisition sensor module output in action data collector module acquisition step S1 is utilized
Process data is transmitted to data filtering module, filters the interference in data；
Imbibition process data after filtering interference are transmitted to CPU module by S4, the data filtering module；
S5, the CPU module handle imbibition process data, obtain the action message of current pipettor
And status information, it is recorded as movement A1 and state S1 respectively；
S6, using action message acquisition sensor module perception pipettor button since being pressed, process second is stopped
Point, last button are released the process between Aligning control, obtain pipettor discharge opeing process data；
S7, action message acquisition sensor module output in the action data collector module acquisition step S6 is utilized
Discharge opeing process data are transmitted to data filtering module, filter the interference in discharge opeing process data；
The discharge opeing process data not interfered with are transferred to the CPU module by S8, the data filtering module；
S9, the CPU module handle discharge opeing process data, obtain the action message of current pipettor
And status information, it is recorded as movement A2 and state S2 respectively；
S10, the CPU module are handled according to movement A1, A2 and state S1, S2 of the pipettor, are sentenced
Settled preceding pipettor completes the entire exercise being once loaded, and is issued by first communication module to the MCU module of sample-adding tracer platform
It is loaded completion notice signal；
After S11, the MCU module for being loaded tracer platform receive sample-adding completion notice signal by second communication module, root
Data are prompted according to pre-set Loading sequence is determining, send control signal to cue module, control cue module adds completion
The state of the plate hole of sample is shown, to indicate that plate hole complete by liquid relief.
In step S11, the cue module passes through one kind or two or more in text prompt, graphical cues and auditory tone cues
Combination carries out state displaying.
In step S10, the CPU module by wireless communication mode or wired communication mode to tracer platform
MCU module issues sample-adding completion notice signal.
Action message described in step S5 and step S9 includes pressure value information suffered by the button of pipettor, pipettor button
Displacement information and pipettor button location information, status information includes liquid volume information in pipettor.
Invention also provides a kind of sample-adding tracer based on the above method, including sample-adding information acquisition device and plus
Sample tracer platform；The action message that the sample-adding information acquisition device includes the first power module for power supply and is sequentially connected electrically
Sensor module, action data collector module, data filtering module, CPU module and first communication module are acquired,
First power module includes the first battery module and acquires respectively with action message acquisition sensor module, action data
The first power management module that device module, data filtering module, CPU module are connected with first communication module；It is described to add
Sample tracer platform includes the second power supply module for power supply and the second communication module being sequentially connected electrically, MCU module and prompt
Module, the second power supply module include the second battery module and respectively with second communication module, MCU module and prompt mould
The second source management module of block connection.
The cue module include text prompt module, graphical cues module and sound module weight one kind or two or more
Combination, wherein text prompt module use LED array, graphical cues module using LCD liquid crystal display, OLED screen and with plate hole pair
One of LED lamp bead answered, sound module use loudspeaker or buzzer.
The first communication module and second communication module use wire communication module or wireless communication module；If first is logical
Believe that module and second communication module use wire communication module, then communication interface between the two is using asynchronous serial port, synchronous string
One of mouth, SPI, I2C, CAN, USB and wired ethernet；If first communication module and second communication module are using wireless
Communication module, the communication frequency band between the two are 400M~500M frequency range, 900M~1000M frequency range, 2.4G frequency range and 5.8G
One of frequency range.
The action message acquisition sensor module uses mechanical switch, geomagnetic sensor, Hall sensor, pressure sensing
One kind or two or more combination in device, infrared sensor and light sensor.
First power module of the sample-adding information acquisition device, action message acquisition sensor module, action data acquisition
Device module, data filtering module, CPU module and first communication module are respectively positioned in the first shell；The sample-adding tracer
Second power supply module, MCU module, cue module and the second communication module of platform are respectively positioned in second housing.
The present invention is based on beneficial effects possessed by its technical solution to be:
(1) method of sample-adding tracer provided by the invention can record respectively at the time of pipettor is completed and takes liquid and liquid relief
Pipettor state determines pipettor liquid relief work completion according to the state at two moment and is prompted, prevents experimental implementation people
Member multiple drain or misses drain to some plate hole in a plate hole；
(2) logic of the method for sample-adding tracer provided by the invention is mainly according to the action logic being manually loaded come to it
The preceding collected pipettor movement carries out logic judgment, i.e., sample-adding is all an imbibition followed by a drain every time, just
It really completes this set of movement and is only primary successfully sample-adding, clear logic；
(3) the method pipettor path default first of sample-adding tracer provided by the invention, after the completion of each plate hole liquid relief, energy
It is enough that suggestion content is directly determined according to pre-set path after generating completion notice signal, it is not necessary to the corresponding plate hole of additional transmissions
Information saves transmission cost；
(4) sample-adding tracer of the invention includes sample-adding tracer platform and sample-adding information acquisition device, and sample-adding tracer platform is available
In placing microwell plate, sample-adding information acquisition device is mountable on pipettor, is responsible for acquiring liquid relief state by sample-adding information acquisition device
Information is responsible for receiving and be shown by sample-adding tracer platform, easy to operate, can be adapted to existing matched pipettor and microwell plate,
It is widely used；
(5) in sample-adding tracer of the invention, sample-adding information acquisition device and sample-adding tracer platform can pass through channel radio
Letter mode transmits data, more flexible compared with wire transmission, avoids influence of the route to experiment table top；
(6) in sample-adding tracer of the invention, sample-adding information acquisition device and sample-adding tracer platform can indicate that and arranged
The plate hole of liquid and i.e. by the plate hole of drain prevents experiment operator from multiple drain or missing to some plate hole in a plate hole
Drain, and structure is simple, easy to operate.
Detailed description of the invention
Fig. 1 is the module diagram of sample-adding tracer of the invention.
Specific embodiment
Present invention will be further explained below with reference to the attached drawings and examples.
The present invention provides a kind of methods for being loaded tracer, comprising the following steps:
The sample-adding path of S1, default pipettor, the sample-adding carried out for pipettor for each plate hole are operated, are repeated respectively
Step S2 to S11；
S2, using action message acquisition sensor module perception pipettor button since being pressed, process first is stopped
Point, last button are released the process between Aligning control, obtain the imbibition process data of pipettor；In this step, pipettor is moved
The relevant movement of liquid is that imbibition acts；
S3, the imbibition of action message acquisition sensor module output in action data collector module acquisition step S1 is utilized
Process data is transmitted to data filtering module, filters the interference in data；In this step, the interference in data includes power supply
Noise, sensor noise etc.；
Imbibition process data after filtering interference are transmitted to CPU module by S4, the data filtering module；
S5, the CPU module handle imbibition process data, obtain the action message of current pipettor
And status information, it is recorded as movement A1 and state S1 respectively；
S6, using action message acquisition sensor module perception pipettor button since being pressed, process second is stopped
Point, last button are released the process between Aligning control, obtain pipettor discharge opeing process data；
S7, action message acquisition sensor module output in the action data collector module acquisition step S5 is utilized
Discharge opeing process data are transmitted to data filtering module, filter the interference in discharge opeing process data；In this step, in data
Interference includes power supply noise, sensor noise etc.；
The data not interfered with are transferred to the CPU module by S7, the data filtering module；
The discharge opeing process data not interfered with are transferred to the CPU module by S8, the data filtering module；
S9, the CPU module handle discharge opeing process data, obtain the action message of current pipettor
And status information, it is recorded as movement A2 and state S2 respectively；
S10, the CPU module are handled according to movement A1, A2 and state S1, S2 of the pipettor, are sentenced
Settled preceding pipettor completes the entire exercise being once loaded, and is issued by first communication module to the MCU module of sample-adding tracer platform
It is loaded completion notice signal；The logic that this step uses is mainly according to the action logic being manually loaded come to collected before
The pipettor movement carries out logic judgment, i.e., sample-adding is all an imbibition followed by a drain every time, is correctly completed this
Set movement is only primary successfully sample-adding；
After S11, the MCU module for being loaded tracer platform receive sample-adding completion notice signal by second communication module, root
Prompt data are determined according to pre-set Loading sequence, and the cue module on microwell plate sends control signal, control prompt mould
Block is shown the state for the plate hole for completing sample-adding, to indicate that plate hole complete by liquid relief.In this step, cue module can be with
The status indicator lamp of plate hole is corresponded to using microwell plate, every time after the completion of sample-adding, respective plate hole status indicator lamp is bright, to show the plate
Hole liquid relief is completed.
In step S11, the cue module passes through one kind or two or more in text prompt, graphical cues and auditory tone cues
Combination carries out state displaying.
In step S10, the CPU module by wireless communication mode or finite communication mode to tracer platform
MCU module issues sample-adding completion notice signal and plate hole serial number data.
Action message described in step S5 and step S9 includes pressure value information suffered by the button of pipettor, pipettor button
Displacement information and pipettor button location information, status information includes liquid volume information in pipettor.
Invention also provides a kind of sample-adding tracer based on the above method, including sample-adding information acquisition device and plus
Sample tracer platform；The action message that the sample-adding information acquisition device includes the first power module for power supply and is sequentially connected electrically
Sensor module, action data collector module, data filtering module, CPU module and first communication module are acquired,
First power module includes the first battery module and acquires respectively with action message acquisition sensor module, action data
The first power management module that device module, data filtering module, CPU module are connected with first communication module；It is described to add
Sample tracer platform includes the second power supply module for power supply and the second communication module being sequentially connected electrically, MCU module and prompt
Module, the second power supply module include the second battery module and respectively with second communication module, MCU module and prompt mould
The second source management module of block connection.
The cue module include text prompt module, graphical cues module and sound module weight one kind or two or more
Combination, wherein text prompt module use LED array, graphical cues module using LCD liquid crystal display, OLED screen and with plate hole pair
One of LED lamp bead answered, sound module use loudspeaker or buzzer.
The first communication module and second communication module use wire communication module or wireless communication module；If first is logical
Believe that module and second communication module use wire communication module, then communication interface between the two is using asynchronous serial port, synchronous string
One of mouth, SPI, I2C, CAN, USB and wired ethernet；If first communication module and second communication module are using wireless
Communication module, the communication frequency band between the two are 400M~500M frequency range, 900M~1000M frequency range, 2.4G frequency range and 5.8G
One of frequency range.
The action message acquisition sensor module uses mechanical switch, geomagnetic sensor, Hall sensor, pressure sensing
One kind or two or more combination in device, infrared sensor and light sensor.
First power module of the sample-adding information acquisition device, action message acquisition sensor module, action data acquisition
Device module, data filtering module, CPU module and first communication module are respectively positioned in the first shell；The sample-adding tracer
Second power supply module, MCU module, cue module and the second communication module of platform are respectively positioned in second housing.
The above examples are only used to illustrate the technical scheme of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these are modified or replace
It changes, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of method for being loaded tracer, it is characterised in that the following steps are included:
The sample-adding path of S1, default pipettor, the sample-adding carried out for pipettor for each plate hole are operated, are respectively repeated steps
S2 to S11；
S2, using action message acquisition sensor module perception pipettor button since being pressed, by the first rest point, most
Button is released the process between Aligning control afterwards, obtains the imbibition process data of pipettor；
S3, the imbibition process of action message acquisition sensor module output in action data collector module acquisition step S2 is utilized
Data are transmitted to data filtering module, filter the interference in data；
Imbibition process data after filtering interference are transmitted to CPU module by S4, the data filtering module；
S5, the CPU module handle imbibition process data, obtain the action message and shape of current pipettor
State information is recorded as movement A1 and state S1 respectively；
S6, using action message acquisition sensor module perception pipettor button since being pressed, by the second rest point, most
Button is released the process between Aligning control afterwards, obtains pipettor discharge opeing process data；
S7, the drain of action message acquisition sensor module output in the action data collector module acquisition step S6 is utilized
Process data is transmitted to data filtering module, filters the interference in discharge opeing process data；
The discharge opeing process data not interfered with are transferred to the CPU module by S8, the data filtering module；
S9, the CPU module handle discharge opeing process data, obtain the action message and shape of current pipettor
State information is recorded as movement A2 and state S2 respectively；
S10, the CPU module are handled according to movement A1, A2 and state S1, S2 of the pipettor, determine to work as
Preceding pipettor completes the entire exercise being once loaded, and issues sample-adding to the MCU module of sample-adding tracer platform by first communication module
Completion notice signal；
After S11, the MCU module for being loaded tracer platform receive sample-adding completion notice signal by second communication module, according to pre-
The determining prompt data of the Loading sequence being first arranged send control signal to cue module, and control cue module is to completion sample-adding
The state of plate hole is shown, to indicate that plate hole complete by liquid relief.
2. the method for sample-adding tracer according to claim 1, it is characterised in that: in step S11, the cue module passes through
One kind or two or more combination in text prompt, graphical cues and auditory tone cues carries out state displaying.
3. the method for sample-adding tracer according to claim 1, it is characterised in that: in step S10, the central processing unit mould
Mode or wired communication mode to the MCU module of tracer platform issue sample-adding completion notice signal to block by wireless communication.
4. the method for sample-adding tracer according to claim 1, it is characterised in that: action message described in step S5 and step S9
Believe the position of pressure value information suffered by button including pipettor, the displacement information of pipettor button and pipettor button
Breath, status information includes liquid volume information in pipettor.
5. a kind of sample-adding tracer based on claim 1 the method, it is characterised in that: including sample-adding information acquisition device and
It is loaded tracer platform；The sample-adding information acquisition device includes the first power module for power supply and the movement being sequentially connected electrically letter
Breath acquisition sensor module, action data collector module, data filtering module, CPU module and the first communication mould
Block, first power module include the first battery module and acquire sensor module, action data with action message respectively
The first power management module that collector module, data filtering module, CPU module are connected with first communication module；Institute
State sample-adding tracer platform include the second power supply module for power supply and the second communication module being sequentially connected electrically, MCU module and
Cue module, the second power supply module include the second battery module and respectively with second communication module, MCU module and prompt
The second source management module of module connection.
6. sample-adding tracer according to claim 5, it is characterised in that: the cue module includes text prompt mould
Combination more than one kind or two kinds of block, graphical cues module and sound module, wherein text prompt module uses LED times
Column, graphical cues module are used using one of LCD liquid crystal display, OLED screen and LED lamp bead corresponding with plate hole, sound module
Loudspeaker or buzzer.
7. sample-adding tracer according to claim 5, it is characterised in that: the first communication module and the second communication mould
Block uses wire communication module or wireless communication module；If first communication module and second communication module use wire communication mould
Block, then communication interface between the two is using in asynchronous serial port, synchronous serial interface, SPI, I2C, CAN, USB and wired ethernet
It is a kind of；If first communication module and second communication module use wireless communication module, the communication frequency band between the two is 400M
One of~500 M frequency ranges, 900M~1000M frequency range, 2.4G frequency range and 5.8G frequency range.
8. sample-adding tracer according to claim 5, it is characterised in that: the action message acquisition sensor module is adopted
With the one kind or two or more group in mechanical switch, Hall sensor, pressure sensor, infrared sensor and light sensor
It closes.
9. sample-adding tracer according to claim 5, it is characterised in that: the first power supply of the sample-adding information acquisition device
Module, action message acquisition sensor module, action data collector module, data filtering module, CPU module and
First communication module is respectively positioned in the first shell；The sample-adding second power supply module of tracer platform, MCU module, cue module and
Second communication module is respectively positioned in second housing.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2017104542883 | 2017-06-14 | ||
| CN201710454288 | 2017-06-14 |
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| Publication Number | Publication Date |
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| CN107688097A CN107688097A (en) | 2018-02-13 |
| CN107688097B true CN107688097B (en) | 2019-04-02 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445247A (en) * | 2018-05-14 | 2018-08-24 | 兰州天启基因生物科技有限公司 | Porous plate locator |
| CN109212249B (en) * | 2018-07-30 | 2021-11-23 | 莫纳（苏州）生物科技有限公司 | Application of sample record appearance |
| CN110806493A (en) * | 2019-09-12 | 2020-02-18 | 南京医科大学 | Micropore plate sample adding indicating system |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE448031B (en) * | 1985-06-18 | 1987-01-12 | Scomas Ab | PROCEDURE AND APPARATUS FOR INDICATING THE DETECTION OF PIPETTE POSITIONS AT A TEST PLATE |
| JPH02284039A (en) * | 1989-04-25 | 1990-11-21 | Kyoto Daiichi Kagaku:Kk | Method for detecting operation state of micropipette |
| US20080031774A1 (en) * | 2006-03-13 | 2008-02-07 | Sage Science, Inc. | Apparatus for Guiding Sample and Reagent Manipulations and Receptacles for Holding Same |
| WO2012158308A2 (en) * | 2011-05-13 | 2012-11-22 | Actrace, Llc | Methods and systems for automated pipette tracking |
| CN106066403A (en) * | 2016-07-01 | 2016-11-02 | 中南民族大学 | A kind of enzyme mark microwell plate loading instruction device |
| CN206168453U (en) * | 2016-11-11 | 2017-05-17 | 中国医科大学 | Countable moves liquid number of times and has liquid -transfering gun of vacuum aspiration function |
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2017
- 2017-08-22 CN CN201710721859.5A patent/CN107688097B/en not_active Expired - Fee Related
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