CN1076859A - The extraction of the flat effective ingredient of the cancer that disappears and dosage form preparation - Google Patents
The extraction of the flat effective ingredient of the cancer that disappears and dosage form preparation Download PDFInfo
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- CN1076859A CN1076859A CN 92102027 CN92102027A CN1076859A CN 1076859 A CN1076859 A CN 1076859A CN 92102027 CN92102027 CN 92102027 CN 92102027 A CN92102027 A CN 92102027A CN 1076859 A CN1076859 A CN 1076859A
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Abstract
The present invention is a kind of production technology of extracting anticancer effective component Radix Fissistigmatis Glaucescentis polysaccharide from Chinese medicine, adopts the water logging bubble, be fried boiling, and precipitate with ethanol, Deproteinization is crossed post, dialysis, precipitate with ethanol, washing is dry and obtain the Radix Fissistigmatis Glaucescentis polysaccharide.Utilize the Radix Fissistigmatis Glaucescentis essence to carry or slightly carry product and make tablet, injection, syrup.This polysaccharide proves antineoplastic effective composition through zoopery repeatedly, and suppression ratio is 70.4%, P＜0.001, nontoxic (LD
5090.4 gram/kg).Mainly esophageal carcinoma, gastric cancer, pulmonary carcinoma, hepatocarcinoma, Hokdkin disease, intestinal cancer, breast carcinoma all there are sure curative effect, avirulence, side effect is also very little.
Description
The invention belongs to the preparation method of from Chinese medicine, extracting anticancer effective division and preparation thereof.
Radix Fissistigmatis Glaucescentis is an Asclepiadaceae milk Lepidium plant, another name: Caulis Marsdeniae Tenacissimae, yellowish banks rose, big bitter rattan, Marsdenia tenacissima, Radix Glycyrrhizae.Formal name used at school Marsdenia tenacissima(Roxb) wight et Arn.Be used as medicine with rattan according to " national Chinese herbal medicine compilation " record Radix Fissistigmatis Glaucescentis, effects such as having cough-relieving, relieving asthma, be anticancer, but its relevant anticancer component is what does not appear in the newspapers, the present invention is a polysaccharide through the anticancer effective component of zoopery proof Radix Fissistigmatis Glaucescentis repeatedly.Yet the extraction process of this anticancer effective component of Radix Fissistigmatis Glaucescentis polysaccharide and the preparation of preparation etc. there is no report, and for this reason, the application has invented the extraction process of Radix Fissistigmatis Glaucescentis polysaccharide and the preparation method of preparation thereof through repeatedly experiment.
Summary of the invention: the extraction of attached Radix Fissistigmatis Glaucescentis polysaccharide and preparation process thereof flow process.
Get the Radix Fissistigmatis Glaucescentis ratan, soaked 8-12 hour, decoct and boiled 2 hours three first time, boiled 1.5 hours for the second time, boiled for the third time 1 hour.Merge three times filtrate.This thick extraction filtrate can be prepared into preparation or continue purification.
The preparation of preparation: three filtrates are concentrated into thick paste shape (every approximately ml contains crude drug 15 grams) adding excipient can be made into dosage forms such as tablet electuary syrup.Or three times thick extracted filtrate and be concentrated into every milliliter and contain crude drug 2.5-3 gram and put cold, add 7-10 and doubly measure 80-95% ethanol cold preservation 24-48 hour, the filtration precipitate that is dissolved in water on filter is regained ethanol, and the precipitate with ethanol defuelling is penetrated water to full dose and transferred pH5.0-7.0 to can be made into injection repeatedly.
Continue purification: three filtrate being condensed into are contained crude drug 2.5-3 gram/ml, add 80-90% alcohol, put filtration in 12-24 hour, precipitate adds the hot water dissolving, the insoluble matter centrifugalize is got supernatant with 95% ethanol repeated precipitation three times, after pure precipitate is dissolved in water, add excessive tannic acid and remove by filter albumen, its filtrate by polyamide column so that excessive tannic acid is removed.Cross post liquid and carry out the flowing water dialysis treatment with semipermeable membrane, dialysis solution is handled with 5: 1 n-butyl alcohol and chloroform mixed liquor, further removes deproteinize, adds 90-95% ethanol then its anticancer effective component is all precipitated.At last with pure precipitate with organic solvent as: acetone, ether wash, drying can obtain the Radix Fissistigmatis Glaucescentis polysaccharide powder.This powder can be made into various dosage forms.
The present invention is its advantage and good effect compared with prior art:
Radix Fissistigmatis Glaucescentis is a Chinese herbal medicine, transports and take equal inconvenience, product are carried or slightly carried to its effective ingredient essence make different dosage forms, its transportation, preserves, takes all conveniently, and dosage is few and accurate, poison pay act on little.
Embodiment 1
Radix Fissistigmatis Glaucescentis powder 1000 grams, added blister 12 hours, and boiled three times, boiled 2 hours for the first time, second decoction was boiled 1.5 hours, the 3rd decoction was boiled 1 hour, filtered merging filtrate and concentrated about 400ml, added 88% ethanol 2500-3000ml, placement is spent the night, is filtered, precipitate adds hot water 400ml dissolving, and is centrifugal, gets supernatant with 95% ethanol repeated precipitation three times.Reclaim ethanol, after precipitate adds the 400ml water dissolution, adding excessive tannic acid filters, filtrate is by getting the amide adsorption column, its post liquid with cellophane flowing water dialysis 1-2 days, dialysis solution adds 5: 1 n-butyl alcohol and the mixed liquor of chloroform filters, filtrate adds 95% ethanol, reclaim ethanol, its precipitate with the washing with acetone after drying promptly.Radix Fissistigmatis Glaucescentis polysaccharide 1 gram.The Radix Fissistigmatis Glaucescentis polysaccharide is made dosage forms such as sheet, pin, syrup by embodiment 2,3,4.
Example 2 Radix Fissistigmatis Glaucescentis injections:
Radix Fissistigmatis Glaucescentis 1000 grams added blister 12 hours, decoct three times, each 1-2 hour, collecting decoction, with 12 layers of filtered through gauze, dope contains crude drug 2.5-3.0 to every milliliter and restrains, and puts cold rnning 7-8 and doubly measures 80-95% ethanol, leaves standstill cold preservation 24-48 hour, filter, on precipitate, add hot water 400ml, continue to filter, reclaim ethanol.Carry out secondary altogether by above-mentioned precipitate with ethanol, add the injection water then to 1000ml, promptly every ml contains crude drug one gram and transfers pH5.0-7.0, uses filtering with microporous membrane, and 100 ℃ of sterilizations of fill peace bottle get final product.
Example 3
Radix Fissistigmatis Glaucescentis 15000 grams, crushing and water-adding bubble 12 hours, be fried boiling three times, each 1-2 hour, collecting decoction filtered, and was concentrated into thick paste shape (about 1000ml) and put cold rnning and go into dextrin (1000 gram) starch (900 restrain) mix homogeneously and make soft material, crossing 16 mesh sieves granulates, 60 ℃ of air-dry, rectifications add magnesium stearate mixing tabletting and get final product, and every 0.3 gram contains crude drug 1.5 grams.
Example 4
Radix Fissistigmatis Glaucescentis 1000 grams are pulverized, and blister was fried boiling three times in 12 hours, each 12 hours.Collecting decoction, filtration are concentrated into about 250ml, and 1 clear liquid is got in cold preservation 12 hours
Syrup (promptly in 350ml water, with sucrose 650 grams, 100 ℃ of dissolving post-heating filter with 80 mesh sieves and make syrup) fully mixes, put cold, add nipalgin 0.5 gram, essence and add aquae destillata in right amount again and stir evenly bottling to 1000ml and promptly get the Radix Fissistigmatis Glaucescentis liquid syrup.
The extraction of Radix Fissistigmatis Glaucescentis polysaccharide and dosage form preparation flow sketch map are as figure
Claims (8)
1, the preparation method of a kind of extraction of effective ingredient of Chinese medicine medicine for preventing and preparation thereof is characterized in that adopting the Radix Fissistigmatis Glaucescentis ratan of Asclepiadaceae milk Lepidium plant is raw material, boils precipitate with ethanol through decocting, Deproteinization is crossed post, dialysis, precipitate with ethanol, washing is dry, and makes various dosage forms.
2, decocting according to claim 1 boils, and it is characterized in that raw material is soaked in water, decocts and boil three times each 1-2 hour, merging filtrate.Be concentrated into the thick paste shape add excipient can be made into sheet, towards dosage forms such as syrup.
3, precipitate with ethanol according to claim 1, it is characterized in that one, with decocting filtrate add 80-90% alcohol precipitation place spend the night, filtering precipitate adds the hot water dissolving, insoluble matter is centrifugal, separate, get supernatant with 85-95% ethanol repeated precipitation three times so that continue with the precipitate purification.Its two, decocting filtrate is concentrated, with 80-95% ethanol precipitation secondary, reclaim ethanol, filtrate adds the injection water, and to transfer PH be 5.0-7.0, can be made into injection.
4, the Deproteinization of removing according to claim 1 is characterized in that pure precipitate is dissolved in water, and adds the centrifugal Deproteinization of removing of excessive tannic acid again.
5, the post of crossing according to claim 1 is characterized in that excessive tannic acid filtrate by polyamide column, so that remove excessive tannic acid.
6, dialysis according to claim 1 is characterized in that mistake post liquid is dialysed with semipermeable membrane flowing water.
7, precipitate with ethanol according to claim 1 is characterized in that dialysis solution is removed Deproteinization with n-butyl alcohol-chloroform mixed liquor of 5: 1 adds 80-95% ethanol then, makes its polysaccharide material precipitation.
8, washing according to claim 1 is characterized in that pure precipitate with organic solvent such as acetone, ether washing, dry powder.This powder adds different excipient and can be made into various dosage forms as sheet, electuary, syrup, injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92102027 CN1076859A (en) | 1992-03-30 | 1992-03-30 | The extraction of the flat effective ingredient of the cancer that disappears and dosage form preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92102027 CN1076859A (en) | 1992-03-30 | 1992-03-30 | The extraction of the flat effective ingredient of the cancer that disappears and dosage form preparation |
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| Publication Number | Publication Date |
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| CN1076859A true CN1076859A (en) | 1993-10-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 92102027 Pending CN1076859A (en) | 1992-03-30 | 1992-03-30 | The extraction of the flat effective ingredient of the cancer that disappears and dosage form preparation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069547C (en) * | 1996-06-18 | 2001-08-15 | 黄德丰 | Gradient extraction technology for extracting multiple active ingredients of medicinal plant |
| CN1310952C (en) * | 2004-06-04 | 2007-04-18 | 山东鲁抗集团中澳生物科技有限公司 | Extraction of polysaccharose of three bristle cudrania |
| CN100362022C (en) * | 2005-11-07 | 2008-01-16 | 中国人民解放军第二军医大学 | Cuttlebone polysaccharide CPS-1 and its preparation and use |
| CN100464759C (en) * | 2003-11-21 | 2009-03-04 | 李文军 | Anticancer Chinese traditional extracts and preparation process and application thereof |
| CN104415073A (en) * | 2013-08-27 | 2015-03-18 | 南京圣和药业股份有限公司 | Application of parenteral medicine preparation to prepare medicines treating MDS |
-
1992
- 1992-03-30 CN CN 92102027 patent/CN1076859A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1069547C (en) * | 1996-06-18 | 2001-08-15 | 黄德丰 | Gradient extraction technology for extracting multiple active ingredients of medicinal plant |
| CN100464759C (en) * | 2003-11-21 | 2009-03-04 | 李文军 | Anticancer Chinese traditional extracts and preparation process and application thereof |
| CN1310952C (en) * | 2004-06-04 | 2007-04-18 | 山东鲁抗集团中澳生物科技有限公司 | Extraction of polysaccharose of three bristle cudrania |
| CN100362022C (en) * | 2005-11-07 | 2008-01-16 | 中国人民解放军第二军医大学 | Cuttlebone polysaccharide CPS-1 and its preparation and use |
| CN104415073A (en) * | 2013-08-27 | 2015-03-18 | 南京圣和药业股份有限公司 | Application of parenteral medicine preparation to prepare medicines treating MDS |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
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