CN107677759A - Dehiscent fruit golden flower TLC Identification - Google Patents
Dehiscent fruit golden flower TLC Identification Download PDFInfo
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- CN107677759A CN107677759A CN201711057071.5A CN201711057071A CN107677759A CN 107677759 A CN107677759 A CN 107677759A CN 201711057071 A CN201711057071 A CN 201711057071A CN 107677759 A CN107677759 A CN 107677759A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
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Abstract
The invention discloses a kind of dehiscent fruit golden flower TLC Identification, with dehiscent fruit golden flower main component chlorogenic acid, 3,5ODCQ and 4,5OThe mixed solution of DCQ is as reference substance solution, with reference to thin-layered chromatography（Chinese Pharmacopoeia version general rule 0502 in 2015）Experiment.The present invention is easy to operate, equipment is simple, colour developing is easy, while deployment rate is fast, and the discriminating for dehiscent fruit golden flower provides foundation.
Description
Technical field
The present invention relates to traditional Chinese medicine authentication technique field, specifically dehiscent fruit golden flower TLC Identification.
Background technology
Dehiscent fruit golden flower（Classification system：Schizomussaenda dehiscens (Craib)H.L. Li）Big shrub, it is high
Up to 7~8 meters；Spray is by strigose, rear nearly hairless, light brown or taupe brown.Leaf papery, lanceolar, long round shape drape over one's shoulders pin
Shape or ovum shape lanceolar.Fringe shape cincinnus basidixed, spends more；Hua Jinwu obstructs；Bract harbors or caducous, narrow；Floral leaf
Ovum shape lanceolar, width egg shape or extremely often to be avette, top is short sharp or blunt, and base portion is wedge-shaped or tapering；The wide obovate of corolla lobe.Capsule
Fruit falls oval or ellipticity obovate；Seed is small, there is corner angle, is covered with small den and groove.5~October of florescence, fruiting period 7~12
Month.Dehiscent fruit golden flower medicinal materials are Rubiaceae dehiscent fruit golden flower platymiscium dehiscent fruits golden flower dry root, stem.
Mussaenda and dehiscent fruit golden flower platymisciums are wide in Guangxi province distribution, using more, the vegetal inspired between different cultivars
State is more close, and its notable feature deforms sepal by a piece of white and several golden yellow little Hua are formed, and saves, overflows during the blossom season
Mountain is all over the fields, very eye-catching, but do not possess the people that professional plant classification is gained knowledge be difficult by the dehiscent fruit golden flower category of different cultivars and
Mussaenda plant makes a distinction.
The content of the invention
It is easy to operate, equipment is simple the invention provides a kind of dehiscent fruit golden flower TLC Identification based on this
List, colour developing are easy, while deployment rate is fast, and the discriminating for dehiscent fruit golden flower provides foundation.
To realize above-mentioned technical purpose, particular content is as follows：
Reference substance：Chlorogenic acid（Zhong Jian institutes provide, and lot number 110753-201415, content is in terms of 96.2%）, 3,5-O- two caffeoyls
Base quininic acid（Zhong Jian institutes provide, and lot number 111782-201405, content is in terms of 92.0%）, 4,5-O- DCQ
（Zhong Jian institutes provide, and lot number 111894-201102, content is in terms of 94.1%）.
Differentiate that step is as follows：
（1）The preparation of need testing solution：The g of dehiscent fruit golden flower powder 1 is taken, adds the mL of methanol 20, is ultrasonically treated 30 minutes, is filtered, filter
Liquid is evaporated, and residue adds the mL of methanol 2 to make dissolving, as need testing solution；
（2）The preparation of reference substance solution：Take chlorogenic acid, 3,5-O- DCQ, 4,5-O- two coffee acyl quinines
Sour reference substance, add methanol that mixed reference substance solutions of every 1mL containing each reference substance 0.5mg is made；
（3）According to thin-layered chromatography（Chinese Pharmacopoeia version general rule 0502 in 2015）Experiment, draw each 5 μ L of above-mentioned solution, put respectively in
On same silica gel g thin-layer plate, with butyl acetate-formic acid-water（3:1:1）Upper solution be solvent, expansion, open up away from 17cm,
Take out, dry.Put and inspected under the nm of ultraviolet lamp 365, in test sample chromatogram, on position corresponding with reference substance chromatogram, Ying Xian
The fluorescence spot of same color；Spray is with the potassium ferricyanide of 1% ferric trichloride ethanol -1%（1:1）Mixed solution（Face with newly matching somebody with somebody）；Daylight
Under inspect, in test sample chromatogram, on position corresponding with reference substance chromatogram, the spot of same color should be shown.
The beneficial effects of the invention are as follows：
The present invention differentiates dehiscent fruit golden flower using TLC Identification, with dehiscent fruit golden flower main component chlorogenic acid, 3,5-O-
DCQ and 4,5-OThe mixed solution of-DCQ can specify beautiful leaf gold as reference substance solution
3 principal spots on flower thin layer collection of illustrative plates are chlorogenic acid, 3,5- respectivelyO- DCQ and 4,5-O- two coffee acyls
Quininic acid.The present invention is easy to operate, equipment is simple, colour developing is easy, while deployment rate is fast, and the discriminating for dehiscent fruit golden flower provides
Foundation, while also the discriminating for dehiscent fruit golden flower category and Radix Mussaendae platymiscium provides reference.
Brief description of the drawings
Fig. 1 is dehiscent fruit golden flower thin-layer chromatogram, and 1 represents mixing reference substance in figure, and 2~5 represent dehiscent fruit golden flower samples,
A represents 3,5-O- DCQ, B represent 4,5-O- DCQ, C represent chlorogenic acid.
Embodiment
The present invention is introduced in order to more detailed, with reference to embodiment, the present invention will be further described.
Embodiment, content are as follows：
Reference substance：Chlorogenic acid（Zhong Jian institutes provide, and lot number 110753-201415, content is in terms of 96.2%）, 3,5-O- two caffeoyls
Base quininic acid（Zhong Jian institutes provide, and lot number 111782-201405, content is in terms of 92.0%）, 4,5-O- DCQ
（Zhong Jian institutes provide, and lot number 111894-201102, content is in terms of 94.1%）.
Differentiate that step is as follows：
（1）The preparation of need testing solution：The g of dehiscent fruit golden flower powder 1 is taken, adds the mL of methanol 20, is ultrasonically treated 30 minutes, is filtered, filter
Liquid is evaporated, and residue adds the mL of methanol 2 to make dissolving, as need testing solution.
（2）The preparation of reference substance solution：Take chlorogenic acid, 3,5-O- DCQ, 4,5-O- two coffee acyls
Quininic acid reference substance, add methanol that mixed reference substance solutions of every 1mL containing each mg of reference substance 0.5 is made.
（3）1% ferric trichloride ethanol solution and 1% potassium ferricyanide solution are prepared respectively, then by both solution with ratio 1:1
Mixing, obtains ferric trichloride ethanol-potassium ferricyanide mixed solution；According to thin-layered chromatography（Chinese Pharmacopoeia version general rule 0502 in 2015）
Experiment, draws each 5 μ L of above-mentioned solution, puts respectively on same silica gel g thin-layer plate, with butyl acetate-formic acid-water（3:1:1）'s
Upper solution is solvent, expansion, opens up away from 17cm, takes out, dry.Put and inspected under the nm of ultraviolet lamp 365, in test sample chromatogram,
On position corresponding with reference substance chromatogram, show the fluorescence spot of same color；Spray is with the potassium ferricyanide of 1% ferric trichloride ethanol -1%
（1:1）Mixed solution（Face with newly matching somebody with somebody）；Inspect under daylight, in test sample chromatogram, on position corresponding with reference substance chromatogram, show
The spot of same color.See Fig. 1.
Claims (1)
1. dehiscent fruit golden flower TLC Identification, it is characterised in that methods described includes herein below：
（1）The preparation of need testing solution：The g of dehiscent fruit golden flower powder 1 is taken, adds the mL of methanol 20, is ultrasonically treated 30 minutes, is filtered, filter
Liquid is evaporated, and residue adds the mL of methanol 2 to make dissolving, as need testing solution；
（2）The preparation of reference substance solution：Take chlorogenic acid, 3,5-O- DCQ, 4,5-O- two coffee acyl quinines
Sour reference substance, add methanol that mixed reference substance solutions of every 1mL containing each reference substance 0.5mg is made；
（3）According to the Chinese Pharmacopoeia thin-layered chromatography of version general rule 0502 experiment in 2015, need testing solution and mixing reference substance are drawn
Each 5 μ L of solution, put on same silica gel g thin-layer plate, using the upper solution of butyl acetate-formic acid-water as solvent, open up respectively
Open, open up away from 17cm, take out, dry；Put and inspected under the nm of ultraviolet lamp 365, spray and mixed with the potassium ferricyanide of 1% ferric trichloride ethanol -1%
Close solution；Inspected under daylight, in test sample chromatogram, on position corresponding with reference substance chromatogram, the spot of same color should be shown.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711057071.5A CN107677759B (en) | 2017-11-01 | 2017-11-01 | The TLC Identification of dehiscent fruit golden flower |
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| CN201711057071.5A CN107677759B (en) | 2017-11-01 | 2017-11-01 | The TLC Identification of dehiscent fruit golden flower |
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| CN107677759B CN107677759B (en) | 2019-11-05 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109709259A (en) * | 2019-02-01 | 2019-05-03 | 贵州医科大学 | A kind of discrimination method of Radix Mussaendae |
| CN112067739A (en) * | 2020-10-13 | 2020-12-11 | 劲牌持正堂药业有限公司 | Identification method and application of thin-layer chromatography of wild chrysanthemum flower medicinal material and formula granules |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1369848A (en) * | 2001-02-13 | 2002-09-18 | 科学与工业研究会 | Method of chromatogram fingerprint atlas, single medicine and preparation standardization |
| CN101363819A (en) * | 2008-01-08 | 2009-02-11 | 广西博科药业有限公司 | Quality control method for yuye qinghuo capsule |
-
2017
- 2017-11-01 CN CN201711057071.5A patent/CN107677759B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1369848A (en) * | 2001-02-13 | 2002-09-18 | 科学与工业研究会 | Method of chromatogram fingerprint atlas, single medicine and preparation standardization |
| CN101363819A (en) * | 2008-01-08 | 2009-02-11 | 广西博科药业有限公司 | Quality control method for yuye qinghuo capsule |
Non-Patent Citations (4)
| Title |
|---|
| XIAO-FANG DENG等: "REVISION OF SCHIZOMUSSAENDA (RUBIACEAE)", 《BLUMEA》 * |
| 李清芳等: "玉叶清火胶囊中玉叶金花鉴别方法的研究", 《数理医药学杂志》 * |
| 黄娇丽等: "百色地区茜草科药用植物的资源调查及研究", 《百色学院学报》 * |
| 龚维等: "基于DNA条形码的玉叶金花属植物鉴定研究", 《中草药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109709259A (en) * | 2019-02-01 | 2019-05-03 | 贵州医科大学 | A kind of discrimination method of Radix Mussaendae |
| CN109709259B (en) * | 2019-02-01 | 2020-07-07 | 贵州医科大学 | Method for identifying mussaenda pubescens |
| CN112067739A (en) * | 2020-10-13 | 2020-12-11 | 劲牌持正堂药业有限公司 | Identification method and application of thin-layer chromatography of wild chrysanthemum flower medicinal material and formula granules |
| CN112067739B (en) * | 2020-10-13 | 2022-04-19 | 劲牌持正堂药业有限公司 | Identification method and application of thin-layer chromatography of wild chrysanthemum flower medicinal material and formula granules |
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| CN107677759B (en) | 2019-11-05 |
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