CN107661783A - A kind of new microfluidic separating chips and preparation method thereof - Google Patents
A kind of new microfluidic separating chips and preparation method thereof Download PDFInfo
- Publication number
- CN107661783A CN107661783A CN201710904326.0A CN201710904326A CN107661783A CN 107661783 A CN107661783 A CN 107661783A CN 201710904326 A CN201710904326 A CN 201710904326A CN 107661783 A CN107661783 A CN 107661783A
- Authority
- CN
- China
- Prior art keywords
- micro
- chip
- separation
- new
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0418—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electro-osmotic flow [EOF]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of new microfluidic separating chips and preparation method thereof, there are micro-structural and microchannel in the micro-fluidic chip surface, original position is prepared for the electroosmotic pump of sample driving, separating the both sides of main microchannel, apply certain electric field, in the presence of electric field, powered different test substance realizes separation, is mainly used in the association area such as separation and analysis.The micro-fluidic chip is simple to operate, realizes the sample introduction of charge species with separating simultaneously on chip, reduce the dosage of reagent and sample, reduce the replacement frequency of capillary, greatly reduce the analysis cost of Capillary Electrophoresis, with portable, economic, fast and efficiently feature, a kind of brand-new microfluidic analysis technology is provided the association area such as to separate and analyzing.
Description
Technical field
The present invention relates to a kind of new microfluidic separating chips and preparation method thereof, there is micro- knot on the micro-fluidic chip surface
Structure and microchannel, original position are prepared for the electroosmotic pump of sample driving, are separating the both sides of main microchannel, are applying certain electric field,
In the presence of electric field, powered different test substance realizes separation, is mainly used in the association area such as separation and analysis.
Background technology
Capillary Electrophoresis is also known as HPCE, be one kind using capillary as split tunnel, with high-voltage dc
For the novel liquid-phase isolation technics of driving force.Capillary Electrophoresis actually comprising electrophoresis, chromatogram and its intersects content, and it makes point
Analysis chemistry is able to from microlitre horizontally entering into a nanoliter level, and makes single cell analysis, or even single molecule analysis is possibly realized.For a long time
Perplex the separation analysis of our large biological molecule such as protein also therefore have new favourable turn.
Capillary Electrophoresis possesses following advantage：Quickly, separation is completed typically within more than ten minutes；It is micro, needed for sample introduction
Sample volume is nL levels；Multi-mode, an instrument can be needed from different clastotypes and only as needed；Economy, experiment disappear
Only several milliliters of cushioning liquid, standing charges are very low for consumption；Automatically, it is the higher separation method of current automaticity.
However, because sample size is few, Capillary Electrophoresis possesses following shortcoming：Preparative capacibility is poor；Due to capillary diameter
It is small, make light path too short, during with some detection method (such as ultraviolet absorption spectroscopies), sensitivity is relatively low；Electric osmose can form because of sample
And change, and then influence separation reappearance.Therefore, develop a kind of convenient, quick, efficient, inexpensive isolation technics, be point
From with analysis etc. field active demand.In recent years, microfluidic chip analysis technology turned into analytical chemistry one it is important
Research direction, it is wherein most active one, either all obtains extensive attention in scientific research or application field.Miniflow
Chip is controlled as a kind of new analysis test platform, there is high flux, integrated, multiple parallel analysis, portable, easy behaviour
Make, low cost and other advantages, be applied widely in various fields.However, using micro-fluidic chip, in its surface system
The analytical technology that standby micro-structural and microchannel, integrated sample introduction and separation are integrated, in related application field, there has been no essence at present
The breakthrough of property.
The content of the invention
It is an object of the invention to provide a kind of new microfluidic separating chips and preparation method thereof, the micro-fluidic chip
There are micro-structural and microchannel in surface, and original position is prepared for the electroosmotic pump of sample driving, is separating the both sides of main microchannel, is applying one
Fixed electric field, in the presence of electric field, powered different test substance realizes separation, is mainly used in separation with the phase such as analyzing
Pass field.
To achieve the above object, the present invention uses following operating procedure：
(1) micro-structural and microchannel figure of each layer chip in micro-fluidic chip are designed and drawn with computer aided design software
Shape.
(2) required micro- knot is processed on each layer micro-fluidic chip substrate surface and adhesive membrane by micro-processing technology
Structure and microchannel, including sample holes, separation main channel and separation subchannel.
(3) capillary of three sections (1 centimetres, 150 microns of diameter) is embedded into electroosmotic pump microflute, carried out with epoxy glue
Sealing is with fixing.
(4) high voltage power supply controller is connected with electroosmotic pump and split tunnel by platinum electrode.
(5) double-deck adhesive membrane is utilized, by the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, composition novel capillary electricity
The micro-fluidic chip of swimming.
In the present invention, the chip substrates of new microfluidic separating chips can be PMMA, PC, PVC, COC, copper, aluminium, stainless steel,
Silicon chip, glass wafer or commercially available all kinds of common CD CDs.
In the present invention, the micro-structural and microchannel of new microfluidic separating chips and adhesive membrane can pass through numerical control mill
Prepared by quarter, laser ablation, LIGA technologies, method of molding, pressure sintering, chemical attack, it is also possible to prepared by Soft lithograph technology.
In the present invention, new microfluidic separating chips are made up of layers of chips, are pasted between each layer chip with adhesive membrane
Close, adhesive membrane can be that double-deck power causes adhesive membrane or common double faced adhesive tape film.
In the present invention, the driving of the sample solution on new microfluidic separating chips relies on EOF caused by electroosmotic pump.
In the present invention, the main microchannel both sides connection high voltage power supply of separation of new microfluidic separating chips, produced by electric field
Raw EOF carries out the separation of charge species.
New microfluidic separating chips proposed by the present invention and preparation method thereof, it is simple to operate, realized simultaneously on chip
The sample introduction of charge species reduces the dosage of reagent and sample, reduces the replacement frequency of capillary, greatly drop with separating
The low analysis cost of Capillary Electrophoresis, there is portable, economic, fast and efficiently feature, lead separation is related with analysis etc.
Had a good application prospect in domain.
The structural representation of Fig. 1 new microfluidic separating chips.
A. voltage control panel, b. electroosmotic pumps, c. separation main channel, d. capillaries, e. ultraviolet detection windows.
Embodiment 1
Micro-structural and the microchannel of the layers of chips of centrifugal type microfludic chip are designed and drawn with computer aided design software
Figure.Micro-structural and microchannel using numerical control CNC system processing two layers of polymethyl methacrylate (PMMA) chip of preparation,
Clean each layer chip with running water, distilled water respectively, and remained with ethanol chip surface fingerprint, the spot such as oil stain.
On double faced adhesive tape film, with carving characters, machining prepares required micro-structural and microchannel.By three sections (1 centimetres, 150 microns of diameter)
Capillary be embedded into electroosmotic pump microflute, sealed with epoxy glue with being fixed.By layers of chips careful alignment, bonding, add
Press seal closes, and the micro-fluidic chip of novel capillary electrophoresis is made.Powered solution is injected into sample introduction hand-hole, applies electric field, it is molten
Liquid enters under electroosmotic pump driving separates main microchannel, in the presence of electric field, the translational speed of the different material to be separated of electric charge
Difference, so as to realize separation, into after detection window, its content is detected respectively.
Embodiment 2
Micro-structural and the microchannel of the layers of chips of centrifugal type microfludic chip are designed and drawn with computer aided design software
Figure.Micro-structural and microchannel using numerical control CNC system processing two layers of disc-shaped makrolon (PC) chip of preparation, respectively
Clean each layer chip with running water, distilled water, and remained with ethanol chip surface fingerprint, the spot such as oil stain.Two-sided
On glue film, with carving characters, machining prepares required micro-structural and microchannel.By the hair of three sections (1 centimetre, 150 microns of diameter)
Tubule is embedded into electroosmotic pump microflute, is sealed with epoxy glue with being fixed.By layers of chips careful alignment, bonding, pressurization envelope
Close, the micro-fluidic chip of novel capillary electrophoresis is made.Powered solution is injected into sample introduction hand-hole, applies electric field, solution is in electricity
Ooze to enter under pump driving and separate main microchannel, in the presence of electric field, the translational speed of the different material to be separated of electric charge is different,
So as to realize separation, into after detection window, its content is detected respectively.
Claims (10)
1. a kind of new microfluidic separating chips and preparation method thereof, there are micro-structural and microchannel in the micro-fluidic chip surface, former
Position is prepared for the electroosmotic pump of sample driving, is separating the both sides of main microchannel, is applying certain electric field, in the presence of electric field,
Powered different test substance realizes separation, is mainly used in the association area such as separation and analysis.
2. new microfluidic separating chips as described in claim 1 and preparation method thereof, it is characterised in that its making step
It is as follows：
(1) micro-structural and microchannel figure of each layer chip in micro-fluidic chip are designed and drawn with computer aided design software
Shape；
(2) processed by micro-processing technology on each layer micro-fluidic chip substrate surface and adhesive membrane required micro-structural and
Microchannel, including sample holes, separation main channel and separation subchannel；
(3) capillary of three sections (1 centimetres, 150 microns of diameter) is embedded into electroosmotic pump microflute, sealed with epoxy glue
With fixation；
(4) high voltage power supply controller is connected with electroosmotic pump and split tunnel by platinum electrode；
(5) double-deck adhesive membrane is utilized, by the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, composition novel capillary electricity
The micro-fluidic chip of swimming.
3. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
The Core Feature device of microfluidic separation chip is micro-fluidic chip, and EOF caused by this chip using electroosmotic pump is used as mixing
The driving force of solution flowing, it can produce in batches, repeatedly utilization, flexible design and assembling.
4. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
Micro-structural and microchannel on microfluidic separation chip be carved by numerical control mill, laser ablation, LIGA technologies, method of molding, hot pressing
Method, chemical attack, the micro-processing method of Soft lithograph technology are prepared on chip substrates surface, and size is in micron level.
5. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
Microfluidic separation chip is formed by stacking by layers of chips, forms the micro-structural and microchannel network of 3 D stereo.
6. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
Microfluidic separation chip can make multigroup micro-structural and microchannel on one chip, form multigroup separative element, can be simultaneously
Multigroup mixed solution is separated, improves the parallel separating capacity of unit interval.
7. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
Microfluidic separation chip is completed to separate the separation of charge species in main microchannel by applying certain electric field.
8. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
What microfluidic separation integrated chip sample introduction and separation were integrated.
9. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
Microfluidic separation chip is adapted to quick separating.
10. new microfluidic separating chips as described in claim 1 or 2 and preparation method thereof, it is characterised in that this new
Type microfluidic separation chip has that portable, economic, quick, efficient, parallel separating capacity is high, sample cross contamination probability is small,
Separate and be with a wide range of applications with analyzing involved numerous association areas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710904326.0A CN107661783A (en) | 2017-09-29 | 2017-09-29 | A kind of new microfluidic separating chips and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710904326.0A CN107661783A (en) | 2017-09-29 | 2017-09-29 | A kind of new microfluidic separating chips and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107661783A true CN107661783A (en) | 2018-02-06 |
Family
ID=61097754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710904326.0A Withdrawn CN107661783A (en) | 2017-09-29 | 2017-09-29 | A kind of new microfluidic separating chips and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107661783A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110193387A (en) * | 2018-10-16 | 2019-09-03 | 长春技特生物技术有限公司 | A kind of totally-enclosed micro-fluidic chip and lotion droplet preparation system |
-
2017
- 2017-09-29 CN CN201710904326.0A patent/CN107661783A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110193387A (en) * | 2018-10-16 | 2019-09-03 | 长春技特生物技术有限公司 | A kind of totally-enclosed micro-fluidic chip and lotion droplet preparation system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Giordano et al. | On-line sample pre-concentration in microfluidic devices: A review | |
| Herr et al. | On-chip coupling of isoelectric focusing and free solution electrophoresis for multidimensional separations | |
| Han et al. | A review: applications of ion transport in micro‐nanofluidic systems based on ion concentration polarization | |
| Turgeon et al. | Micro free-flow electrophoresis: theory and applications | |
| Lin et al. | Sample preconcentration in microfluidic devices | |
| US5770029A (en) | Integrated electrophoretic microdevices | |
| Graß et al. | A new PMMA-microchip device for isotachophoresis with integrated conductivity detector | |
| Kopp et al. | Developments in technology and applications of microsystems | |
| Zhang et al. | Narrow sample channel injectors for capillary electrophoresis on microchips | |
| Chen et al. | A prototype two-dimensional capillary electrophoresis system fabricated in poly (dimethylsiloxane) | |
| Novo et al. | Current advances and challenges in microfluidic free-flow electrophoresis—A critical review | |
| Rocklin et al. | A microfabricated fluidic device for performing two-dimensional liquid-phase separations | |
| US9039973B2 (en) | Hybrid digital and channel microfluidic devices and methods of use thereof | |
| CN103055973A (en) | Novel micro-fluidic separating chip and preparation method thereof | |
| Kler et al. | Column–coupling strategies for multidimensional electrophoretic separation techniques | |
| US20110220498A1 (en) | Method for Building Massively-Parallel Preconcentration Device for Multiplexed, High-Throughput Applications | |
| Ma et al. | Integrated isotachophoretic preconcentration with zone electrophoresis separation on a quartz microchip for UV detection of flavonoids | |
| CN101013083A (en) | Optical fibre embedded low-voltage actuated capillary tube electrophoresis chip | |
| CN107661783A (en) | A kind of new microfluidic separating chips and preparation method thereof | |
| Kuo et al. | A microfabricated CE chip for DNA pre‐concentration and separation utilizing a normally closed valve | |
| Kinde et al. | A microfluidic SPLITT device for fractionating low-molecular weight samples | |
| US20090250345A1 (en) | Microfluidic electroelution devices & processes | |
| Park et al. | Ion bridges in microfluidic systems | |
| US20050133371A1 (en) | Apparatus and method for Edman degradation on a microfluidic device utilizing an electroosmotic flow pump | |
| Graß et al. | Micro-structured analytical instrumentation for the analysis of liquids |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180206 |